Plasmid DNA Purification Kit
Contents
1. OD320 OD280 OD320 is between 1 7 2 0 RELATED PRODUCTS EZLys Bacterial Protein Extraction Reagent K8001 100 500 Ampicillin Sodium 2484 5G 25G 100G Carbenicillin disodium 2485 1G 5G 10G Ethidium Bromide 1203 10 Plant Genomic DNA Extraction Kit K2800 100 Bacterial Genomic DNA Extraction Kit K2801 100 Viral DNA RNA Extraction Kit K2803 100 Whole Blood Genomic DNA Extraction and Purification Kit K2804 100 Mitochondrial DNA Isolation Kit K280 50 Genomic DNA Isolation Kit K281 50 FOR RESEARCH USE ONLY Not to be used on humans Tel 408 493 1800 Fax 408 493 1801 www biovision com tech biovision com2. 2 5 ml for Low copy plasmids overnight bacterial culture in a tube and centrifuge at 12 000 x g for 2 min at 4 C to collect cells Remove supernatant completely This can affect the purity of the plasmid DNA 2 Add 100 ul Buffer A and completely resuspend cells making sure there are no clumps Vortex if needed to loosen the pellet Add 100 ul Buffer B and mix for 3 mins until the solution becomes clear Then add 75 ul Buffer C mix by rotating and let it stand for 2 mins to neutralize 3 Centrifuge at 12 000 x g for 10 mins and transfer the supernatant into a fresh tube B Binding 1 Add 5 ul re suspended magnetic beads and 250 ul Isopropanol user provided to the tube Mix by inverting rocking the tube for 5 mins DO NOT Vortex 2 Separate the beads by the magnetic separator BioVision Cat 1999 1 and remove the clear supernatant residual liquid BioVision Incorporated 155 S Milpitas Boulevard Milpitas CA 95035 USA 04 14 For research use only C Washing 1 Add 500 ul wash solution mix for 1 2 mins Repeat magnetic separation and remove supernatant Dry the beads at RT for 5 mins D Elution 1 Add 100 ul Elution buffer pipette up and down slowly to mix incubate at 65 C for 10 mins Rock the tube gently every 2 3 min 2 Repeat magnetic separation and transfer the supernatant to a fresh tube carefully for downstream experiments 3 Store the extracted DNA at 20 C for future use Note Generally OD260
3. BioVision Plasmid DNA Purification Kit Catalog K2802 200 200 preparations Store at 4 C l Introduction BioVision s plasmid DNA extraction and purification kit uses a unique system of magnetic beads and buffers to extract highly pure plasmid DNA from low or high copy plasmids This kit does not involve the use of any toxic substances It is safe convenient and suitable for high throughput automated platforms DNA purified by this kit can be used for a variety of downstream molecular biology applications such as cloning PCR and more Kit Contents K2802 200 Component 200 preparations Part Number Buffer A 20 ml K2802 200 1 Buffer B 20 ml K2802 200 2 Buffer C 15 ml K2802 200 3 Magnetic beads 1ml K2802 200 4 Wash solution 30 ml K2802 200 5 Elution Buffer 20 ml K2802 200 6 General Considerations and Reagent Preparation e Read the entire protocol before beginning the procedure e Incubate Buffer B in a 37 C water bath before use until any white precipitate dissolves Mix gently e Mix the magnetic beads to make a homogeneous suspension before use e Add 200 ul of RNase A 10 mg ml to Buffer A and 0 16 g NaOH to Buffer B before use e Add 80 ml absolute ethyl alcohol user provided to wash solution and mix before use e If the volume of bacterial culture is increased the volume of magnetic beads should be scaled up proportionally Bacterial DNA Extraction Protocol A Lysis 1 Take 1 2 ml
Download Pdf Manuals
Related Search