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1. Display Scale function Label function Heading function Display samples function are identical to those of Wavelength Scan mode See paragraph 9 8 page 58 82 Lab Power software for Uvi Light amp UVIKON 12 WAVELENGTH PROGRAM VAN anne Always save the data AND the methods in mode Wavelength Program The beforehand saved data could be reloaded only if the wavelengths of the file and the current wavelengths are identical 12 1 CHOICE OF THE METHOD File Methods Parameters Execute View Utilities Report Window Help Ls 4 X RI oad User Method m If Bl d Fixed Wavelength a Sapa 4260 alo Wavelength Scan fal Off Click on Methods New method and Wavelength Program 12 2 CHOICE OF ACQUISITION PARAMETERS Click on Parameters et Acquisition The following dialog box appears Parameters Wavelength Program Acquisition Ma of wavelength 2 14 E Wavelength values nm Set Delay time 0 0 3000 0 s jo Averaging time 0 1 60 0 s i 2 No of Cycles 1 99 i m Cycle time 0 0 900 0min fo Number of samples 1 6 i Cancel No of wavelength Number of measuring wavelength max 14 83 Lab Power software for Uvi Light amp UVIKON Wavelength values Click on Set Enter the wavelengths values Delay time It is the time between the moment where th
2. Click on Methods New Method and Fixed wavelength 8 2 CHOICE OF ACQUISITION PARAMETERS Click on Parameters et Acquisition The following dialog box appears Parameters Fixed Wavelength Acquisition Wavelength 180 0 900 0 nm Delay time 0 0 3000 0 Averaging time 0 1 60 0 s Number of samples 1 10 noe Wavelength Choice of the measuring wavelength Delay time It is the time between the moment where the operator started measurement and the moment where the real measurement will be carried out Average time Defines the time in second used by the unit to gather the data and to calculate an average value on a reading During measurements with strong absorbance to reduce the noise it is recommended to use a high value Number of samples Fix the number of samples to be measured per series If a cell changer is used the cuvettes are measured automatically 43 Lab Power software for Uvi Light amp UVIKON 8 3 FILE OPENING Click on File et New It displays ajala E solo ule acaparar Fou ses ei c Display amp PR eng TEL em Fi Medea Value At TO Ha of samples E Special Lern u nicer T A default file will be open and its name will be Fixed1 Measure Pemes 0 8 4 CHOICE OF CALCULATION PARAMETERS Click on Parameters and
3. Select the type of calibration curve i e Linear Regression Click on Load bens Fiscd Wawelenglh p Vues Select the calibration curve previously carried out i e CALIBO1 con Click on Open 104 Lab Power software for Uvi Light amp UVIKON Calculation Parameters Fixed Wavelength Calculation Values Reference CALIBOT C Off Linear Reg C Quadratic Regr Units Coefficient 0 0104431137 Coefficient B 0033558241 7 Interpolation C Concentration C Functions Llose Cancel Curve parameters are displayed in windows labeled coefficient A and coefficient B Select the units Click on Close 13 1 2 5 Method backup Me Meta In VRR gt Dl Fic Del Hl gth a zjm mie m Elala H elc ajel O AE t Select Methods and Save Method Sk Fixed Wavelength miej m i a a 215 m gj Save the method i e CALIBO1 pfx 105 Lab Power software for Uvi Light amp UVIKON 13 1 2 6 Samples measurement lt Measure made with a single cell holder See chapter 14 to use a cell changer Specific command for Uvi Light Insert the blank solution in the single cell holder Click on Execute and AutoZero or click on the icon AZ Insert the first sample C
4. WARNING Total sucking up volume Sample volume Dead volume 123 Lab Power software for Uvi Light amp UVIKON Also if the operator wants to separate the samples by air reduction of the contamination inter samples it must fill its tube with the exact quantity with sample which it programmed in the parameters of the peristaltic pump The peristaltic pump will suck up first the sample sample volume then the air dead volume 15 1 4 Measurement Use MANUAL position of the peristaltic pump button to fill the flowthrough cell with the blank solution Click on the icon AZ to perform the zero Select Execute and Fill Sipper or click on the icon Ed to start the sample automatic sucking up After the sucking up and the delay the sample measurement will be automatic he operator will be able to measure again the same sample by clicking on the icon m There will not be new sucking up 15 1 5 Backup of sipper parameters The sucking up parameters will be saved except the dead volume with the analysis parameters 15 2 SPECIFIC COMMAND FOR UVIKON XL XS The automatic sucking up is based on a system of peristaltic pump with a pump of tube cassette and an easy replacement of the pipe All the functions of the sucking up can be controlled via the tools bar of the Lab Power software 15 2 1 Installation Switch off the spectrophotometer and connect the item cable on the connector of the
5. Calculation Derivative Normalization Derivative Order 1 Smoothing Level Lowy Off C Peak Detection C Curve Operations Functions Derivative C Smoothing omen Calculate directly the 1 to 4 order derivative Derivative Order Enter the derivative order from 1 to 4 Derivative Normalization Allows aligning the largest peak of derivative on the largest peak of the original curve Smoothing Level Allow to smooth plus or minus the derivative curve Three smoothing algorithms are available Low medium and high 9 4 5 Smoothing CalculationParameters Wavelength Scan Calculation C Off C Peak Detection C Curve Operations Smoothing Level C Functions C Derivative Smoothing caes Allow to smooth plus or minus the curve Three smoothing algorithms are available Low medium and high The calculation selected in Calculation Parameters will be executed on the curves by clicking on Execute and Calculate or by pressing F3 key or by clicking on the icon i 55 Lab Power software for Uvi Light amp UVIKON 9 5 METHOD BACKUP LabPower Junior WSCAN1 leix Help User Method Method ave Method W wscani Measure Bi xj Click on Methods and Save method or press F4 key 21x Enregistrer dans Work Bir Nom dufichier fietT1 SC S lt
6. Calculation Calculation Parameters Fixed Wavelength Calculation Values te Off C Linear Reg C Quadratic Reor Interpolation C Concentration C Functions Cs Several types of calculation can be applied to the values of reading absorbance to calculate the concentrations Linear Regression Quadratic regression Linear Interpolation Concentration Functions 44 Lab Power software for Uvi Light amp UVIKON 8 4 1 Linear regression Calculation Parameters Fixed Wavelength X Values Reference CALLURYE Calculation C Of Linear Reg C Quadratic Regr Units Coefficient 00364425911 Coefficient B 0 0504068757 Interpolation C Concentration C Functions Close Cancel Calculation Y AX B Y Absorbance X Concentration Enter values of coefficients A and B if they are known or choose a calibration curve taking again this calculation by clicking on the button Load and by choosing the file comprising this curve Choose the units Click on Close 8 4 2 Quadratic regression Calculation Parameters Fixed Wavelength X Calculation Values Reference CALCURVE C Off C Linear Reg Quadratic Reg Units Coefficient A 0 00008721 39 Coefficient B 1 0433251939 Coefficient C 0 0282404002 Canoa C Interpolation C Concentration C Functions Calculation Y AX BX C Y Absorb
7. Shift or one by one mouse left click Ctrl Enter a different number for each curve 58 Lab Power software for Uvi Light amp UVIKON If the selected file includes several curves the following dialog box appears Load sample X W Sample 1 to m Sample 2 to e Sample 3 to a W Sample 4 to EN Select the Samples to load enabling the corresponding check bos and write the new sample number in the edit box Select one or several curves to merge with the one on the screen Give to the curve a different number from that of the already displayed curve Click on OK 9 8 2 Slitting of a multi curves files The multi curves files acquired in particular during the use of a cell changer can be separate in individual single curve files This separation is made automatically when the user saves his multi curves file with the menu Save as The software creates a directory with the name of the multi curves file follow up of the mention splitted Inside this repertory are saved individually the curves each one with the name multi curves file incremented with an index Example WORK L TESTWOI L TESTWO1 01 scn L TESTWO2 02 scn olx Fichier Edition Affichage Favoris Outils au Q Pr c dente A Rechercher Dossiers y X 19 y Adresse a I o Dossiers Date de modification 3 Systran r 5 TESTWO1 01 scn 1 Ko remet ScanFi 22 02
8. CALIBO1 rep and click on Save 13 1 2 11 Printing J LabPower Junior CALIBO2 LI File Methods Parameters Execute View Utilities Report Window Help in I dE 10 en mgl Bee AZ Go ajaj CALIBO1 Bcau02 2 Report zin E Measured values Absorbance Calculated values Lin Reg Operator No Wavel Value S No Wavel Value 1 580 00 1 4503 1 1 580 00 1 4482 Heading FIXED WAVELENGTH 2 580 00 1 4503 1 2 580 00 1 4482 PARAMETERS LAMP CHANGE nm BANDWIDTH nm AUTOPRINT AUTOSAVE File mode FIXED WAVELENGTH Sample No of Measurements 2 Measured values Absorbance No Wavel Value_S 1 580 00 1 4503_1 2 580 00 1 4503_1 Calculated values Lin Reg No Wavel Measure_S Calculation 1 580 00 1 4503_1 1 4482 2 580 00 1 4503_1 1 4482 Acquisition Parameters i i Spectrophotometer informations Wavelength nm 580 0 Print Mode Values Wavelength nm Delay s Poo Averaging time s 1 0 No of samples Sans titre Paint Not connected 109 Lab Power software for Uvi Light amp UVIKON Select the data window to print Report window in the above example Select File and Print 13 1 2 12 Summary of the backup files In this example File of calibration method CALIBO1 pcf File of calibration curve CALIBO1 con File of measuring method CALIBO1 pfx File of results calculated values CALIBO1 fix File of results read values CALIBO2 fix File of
9. Click on WL Pairs 86 Lab Power software for Uvi Light amp UVIKON Wavelength Pairs q X Wavelength 1 nam Wavelength 2 nm Wavelength Pair 1 405 0 360 0 Wavelength Pair 2 IN ADR 0 3 340 0 Cancel Wen Wavelengths Enter a number here between 1 and 3 which corresponds to the order of WL choice NOTE To remember the choice order of the WL click on View Wavelengths The wavelength corresponding to the number of choice will appear only once the user validated his choice by OK Run the measurement The results will be displayed as below 9 LabPower Junior ONECYCLE l l x Fie Methods Parameters Execute View Utilities Report Window Help alal E Az Co ajaj D c 5 25 a E Wavelength Program mS E a xr Caic oft a jem fal Difference RE Measured values Absorbance Calculated values WLFunction Difference No Wavel alue Cycle 1 Cycle 1 1 405 00 1 6320_1 Sample 1 Sample 1 D 1 3 2 360 00 1 6730_1 D 340 00 0 1130_1 0 0410 1 5190 12 4 2 Ratio measurement The measurement progress of ratio is made in the same way that the difference measurement Select Ratio in place of Difference for Function 12 4 3 Concentration measurement Example 3 wavelength WL1 405 nm WL2 360 nm and WL3 340 nm Calculation 2 4xWL1 0 5xWL2 and 2 4xWL1 0 34xWL3 Enter 3 in No of wavelength
10. Optics Lamp Change 230 400 nm EE Bandwidth nm 5 Lamp Change 290 400 nm It is the wavelength of commutation between deuterium lamp and halogen lamp All values between 290 and 400 nm are available by default 338 nm Bandwidth Spectrophotometer bandwidth expressed in nanometers lt is automatically detected by the software 2 or 5 nm and can not be changed by the user 27 Lab Power software for Uvi Light amp UVIKON Specific command for UVIKON XS The dialog box which displays the parameters appears Basic parameters El Optics Automation Printout Optics Lamp Change 290 400 nm y Bandwidth nm hs Response Time s 002 Fiter Change 700 850 nm ro Filter Change 540 580 nm 570 Filter Change 470 490 nm jaso Fiter Change 340 380 rm 70 Fiter Change 250 300 nm 280 Cancel Lamp Change 290 400 nm It is the wavelength of commutation between deuterium lamp and halogen lamp All values between 290 and 400 nm are available by default 338 nm Bandwidth Spectrophotometer bandwidth expressed in nanometers lt is automatically detected by the software 1 8 nm and can not be changed by the user Response Time It is the time between two data displaying on the screen Filter Change Each one of these parameters preset a wavelengths range in which the user can change the point of commutation of the filters which cut the stra
11. The way to reach the programming of acquisition parameters is identical whatever the method of measurement used On the other hand the acquisition parameters themselves are specific to each method of measurement Refer to each one of them for more explanations To have access to these parameters acquisition select Parameters and Acquisition File Methods Parameters Execute View Utilities Report Window C Ev etengtn m a laa alar az mim Calculation Cell Changer gt Stirrer gt Sipper Sample Changer gt 6 3 CHOICE OF CALCULATION PARAMETERS The way to reach the programming of calculation parameters is identical whatever the method of measurement used On the other hand the calculation parameters themselves are specific to each method of measurement Refer to each one of them for more explanations To have access to these parameters acquisition select Parameters and Calculation 26 Lab Power software for Uvi Light amp UVIKON u Lahr Junior FIXED Fle Methods Execute Wew Unites Report Window E Wavelength mic au E ajaj HJ sz o ajaj erar wil me E PL rer 6 4 CHOICE OF BASIC PARAMETERS Select Parameters and Basic 6 4 1 Optics index gt lt Specific command for Uvi Light The dialog box which displays the parameters appears Basic parameters Optics Automation Printout
12. 131 18 1 SPECIFIC COMMAND FOR UVIKON XS XL eese nnne nnne nnn nnns 131 ON PM oo P Ec 131 lods LHERMOSYSIEM diS Play HO aan a E a E Bea 132 18 1 3 Remote control or local y MOLE A ance bas ieee 132 SAR LHERMOSYSTEM JUNCOS ne an ee 132 MAT UU m CE 133 19 THERMOPACR 24 250 ae mega EO bv dE EHI OW In 135 Lab Power software for Uvi Light amp UVIKON 1 REMARKS The Automatic aspiration system kit is manufactured by SECOMAM The information contained in this document may be the object of patents or patent applications by SECOMAM The possession of this document does not confer any license to such patents The following names are SECOMAM s registered trademarks throughout the world SECOMAM Lab Power Uvi Light UVIKON This manual is updated periodically The updates are included in the new editions All information supplied in this edition of the manual may be amended before the products described herein are available All reproduction rights reserved Lab Power software for Uvi Light amp UVIKON 2 WARRANTY The new equipment and material sold by SCHOTT INSTRUMENTS is guaranteed against any manufacturing defects for one year unless otherwise stated by SCHOTT INSTRUMENTS with effect From the technical acceptance of the equipment in the factory by the buyer or his designee or failing this For Metropolitan France from the date on the delivery note For other destinations from t
13. 5 pri ES wscan pra YELLOW Ei derrivative Ex SAMARIUM E srour testi Give a name to the file and click on Save The file extension will depend of the used methods Wavelength scan Fixed wavelength 6 10 DATA FILE OPENING If the principal page of a method is opened and that the line of state indicates Ready it is possible to open a data file safeguarded beforehand Click on File then Open or press Ctrl O The following dialog box appears 21x Regarder dans 3 Work da t E EE 3 Splitted ES MEASP Ei test1WJ Stestiww Splitted E Peak test1WW 400500 pri E wscan 425475 pr2 YELLOW Ei derrivative SAMARIUM EJ GROUP testl Mam du fichier I Bw Fichiers detype DaawiscANCs rj EA o 32 Lab Power software for Uvi Light amp UVIKON The displayed list represents all data files made with the selected method Select the file to open and click on Open 6 11 DATA EXPORT 6 11 1 ASCII asc format The files stored on the disc can be converted in ASCII format Many types of software text processing spreadsheets data bases management statistical programs accept data with the ASCII format Choose File Export and ASCII Choose the directory give a name to the file and make Save 6 11 2 EXCEL xls format The files stored on the disc can be converted in Excel format XLS to be used
14. 5 4 1 Starting According to the type of spectrophotometer follow the procedure previously described 5 4 2 System autotest and initialization During the autotest we can see the following picture UV VIS Spectrophotometer Copyright 1999 SECOMAM Initializing instrument f SE TZ AE A PRAT The autotest lasts approximately 2 minutes max and carries out following controls e Data memory e The switching on of the Tungsten halogen lamp e he switching on of the Deuterium lamp e Data transfer e Run of wavelength When the unit is detected the main window appears on the screen A message on the bottom of the screen indicates the unit status Initialization during autotest Ready when autotest is over When the autotest is finished the following window appears on the screen 20 Lab Power software for Uvi Light amp UVIKON File Methods Parameters Execute View Utilities Report Window Help Dell Fixed Wavelength Mi ala El Al FI az Go Acquisition Parameters Display amp Print Instant measure Wavelength nm 580 0 Print Mode Values Wavelength nm Absorbance AU Delay s 00 Averaging time s 1 0 No of samples 1 580 gt 00 0 0 1 43 Ready Pf 5 4 3 Stopping of the unit lt Specific command for Uvi Light Click on File and Exit or press ALT F4 Switch off the spectrophotometer Specific command for UVIKON XL XS Click on t
15. CALIBRATION 13 1 CALIBRATION IN FIXED WAVELENGTH Example Development of a calibration curve and measurement of an unknown sample 13 1 1 Calibration curve 13 1 1 1 Choice of the method Click on Methods New Method Calibration and Fixed wavelength 13 1 1 2 Choice of acquisition parameters Fichier Methode Param tres Actions Voir Utilitaires Rapport Fen tre AAA A A Az R gre Basique Multicuves gt Sipper Click on Parameters et Acquisition The following dialog box appears 95 Lab Power software for Uvi Light amp UVIKON Parameters Fixed Wavelength Acquisition Wavelength 180 0 300 0 nm Delay time 0 0 3000 0 s 003 Averaging time 0 1 60 05 103 Number of samples 1 10 1 ceca Wavelength Choice of the measuring wavelength Delay time It is the time between the moment where the operator started measurement and the moment where the real measurement will be carried out Average time Defines the time in second used by the unit to gather the data and to calculate an average value on a reading During measurements with strong absorbance to reduce the noise it is recommended to use a high value Number of samples Fix the number of samples to be measured per series If a cell changer is used the cuvettes are measured automatically 13 1 1 3 File opening Click on File et New It displays
16. Calculations Acquisition Parameters Display amp Print Instant measure ScanRange nm 180 580 Print Mode Values Wavelength nm Absorbance AU Data Interval nm 1 00 Cycles N min 1 0 0 Graph Range Y Autorange 5 80 00 0 0 1 52 Scan Speed nm min 200 No of samples BE Graph RangeX 300 500 z a Rady EF Title bar Contains the name of the currently open document A document is a data file loaded or measured with any user or standard method Menu bar Contains the list of the commands allowed for the currently open document Commands which are not allowed are displayed in grey Main Tool bar Displays the current method and the optional user method name Contains buttons as shortcuts for different menu commands Calculation Tool bar Displays the current calculation mode and contains buttons as shortcuts for menu commands regarding calculations Parameter bar Displays the current acquisition parameters and the instant measure 25 Lab Power software for Uvi Light amp UVIKON Document windows Each measure employs a different child window that contains measured data in table or graph format The format can be changed selecting the menu View Table or View Graph or clicking the corresponding button of the main tool bar Status bar The status line always shows the current instrument status error messages and a short help text 6 2 CHOICE OF ACQUISITION PARAMETERS
17. Dely 3 00 Sampling Flaielimint amp Gesch Range r Auborange Meus Tere finn 100 Ma osamples 1 Graph 20 On the left of the screen is displayed the curve of the reaction with the linear regression line On the right of the screen are displayed the number of the sample the activity slope of the straight regression line multiplied by the factor the standard deviation and the determination coefficient 10 7 RESULTS BACKUP Click on File and Save as LabPowerJ xj Press YES to save calculated values Press NO to save measured values ou non anuer Click on Non to save the measured values here the curve Click on Oui to save the calculated values here the results the activity Example Curve tim for raw absorbance values versus time Results tim for the results activity standard deviation 74 Lab Power software for Uvi Light amp UVIKON 10 8 CURVES OPERATIONS The following functions Curves merging Splitting of a multi curves file in individual files Cursor function Zoom function Absorbance Transmittance function Graphic Table function Display Scale function Label function Heading function Display samples function are identical to those of Wavelength Scan mode See paragraph 9 8 page 58 75 Lab Power software for Uv
18. J LabPower Junior FWSTD1 File Methods Parameters Execute View Utilities Report Window Help Do 5 E E cal Fixed Wavelength ao e ala 26 mr Es FWSTD1 Calibration data Wavelength nm 580 0 No Nominal Measured_S 1 Acquisition Parameters Wavelength nm 580 0 e Delay s 00 Averaging time s 1 0 Dae Spectrophotometer informations Print Mode Values PR A default file will be open and its name will be FWSTD1 Calibration data No of samples Not connected fff 96 Lab Power software for Uvi Light amp UVIKON 13 1 1 4 Choice of calculation parameters The choice of calculation parameters is initially limited to enter the values of standards concentration Select Parameters and Calculation or File and Edit Standards Edit Calibration File Nominal Measured Sample Ma mo oF DD M E c Pi co r Delete all measures Delete Line coa Enter standards values Click on Close 13 1 1 5 Choice of calculation mode Y LabPower Junior FWSTD2 Fie Methods Parameters Execute View Utilities Report Window e 58 Ei Cal Fixed Wavelength ma E E Yrawelength nm 580 0 No Nominal Measured 5 1 10 000 2 20 000 3 40 000 4 60 000 Select a calculation mode for the curve Linear regression quadratic regression or interpolation 97 Lab Power
19. Measured_5 1 10 000 2 20 000 3 4 40 000 00 000 Run the calculation of the calibration curve by selecting Execute and Calculation or by clicking on the icon ix Wiewelength om 500 0 Conc Factor 73 5701 Lin Aleg Cort A 0 016 B 0 0736 Quad Fleg Coell As 0 0001 Be 0 0484 C 0 0405 in Nominal Measured x 10 000 0 1561_1 20 000 0 2504 1 1 E 3 40 000 0 5708 1 4 30 000 0 4906_1 Once calculation carried out following information is displayed the measuring wavelength the concentration factor the A and B factors of linear regression line Ax B the A B C factors of the quadratic regression curve Ax Bx C 13 1 1 8 Displaying of calibration curve Displayed the curve by selecting View Graphic or by clicking on Power Junior SWATCILpct F e Methods Parameters Execute View Ultftes Report Window Dell 8 d Cat leegh miej ama 2 4 E z oo ale 99 Lab Power software for Uvi Light amp UVIKON To change the calibration curve select another type of calculation then select Execute s l Ac and Calculate or click on the icon Jl F LabPower Junior SWATCH pcf 0 i 55 25 L i 9 Cal Fixed Wavelength i e Quadratic regression Y LabPower Junior SWATCH pcf File Methods Parameters Execute View Utilities Report Window A o D HEBEL bel LT y AxHBx C Bi CALIBO1 pcf Calib
20. Methods New method and Time Drive 10 2 CHOICE OF ACQUISITION PARAMETERS Click on Parameters et Acquisition The following dialog box appears Parameters Time Drive Acquisition Wavelength 190 1100 nm Sampling Rate min Delay 0 0 3000 02 Measure time 0 1 900 0 min 10 No of Samples 1 6 E Cancel Wavelength Choice of the measuring wavelength Sampling Rate Fix the number of points measured per minute High frequencies of acquisition can be used only for short measurements for example fast reactions where a high resolution is necessary In other hand use low frequencies for long durations of measurement what will reduce the noise and the treatment duration 69 Lab Power software for Uvi Light amp UVIKON Delay It is the time between the moment where the operator started measurement and the moment where the real measurement will be carried out Measure time It is the total duration of measurement in minute If the time of measurement must be raised it will be advisable to reduce beforehand the sampling rate before being able to enter the total time of measurement Number of samples Fix the number of samples to be measured per series lf a cell changer is used the cuvettes are measured automatically 10 3 FILE OPENING Click on File et New It displays File Methods Parameters Execute View Utilities Report Window Help Do i
21. PUES de Wavelength m z E recs El Az Go e mul g ale ca ge sz Ele EA Click on Parameters and Sipper Select On 15 1 2 Sipper adjustment File Methods Parameters Execute View Utilities Report Window Deiskelalal 2 i fe lala E az co e zal EE calc ort Break i Pt Click on Execute and Sipper Calibration 121 Lab Power software for Uvi Light amp UVIKON Sipper Calibration X Sipping volume Calibration volume ml z Sipped volume Confirm with and buttons if sipped volume iz higher ar lower than desired valume Ls Ea Speed plsec a Hun test OR Cancel Enter 1 ml for Calibration volume Enter 150 ul sec for the Speed Prepare 5 tubes with each one 2000 ul of distilled water and a sixth with 1000 distilled water ul It will be used as standard Present the first tube with 2000 distilled water ul and to click on Run test The instrument sucks up a certain quantity of liquid Compare the remaining volume in the tube with the 1000 ul of the standard tube If the remaining volume is different from the volume of the standard tube change speed ul s with the keys or NOTE As the speed of the peristaltic pump is constant it is the time of sucking up which will change If the sucked up volume is too large the operator will increase speed then the time of sucking up will be decreas
22. Save Automatic Print Automatic Save Save automatically the data the methods or the calibrations in the repertories beforehand defined in Utilities Preferences and Directories AutomaticPrint 29 Lab Power software for Uvi Light amp UVIKON Print automatically the data selected in the index Printout when the user closes the active windows of results 6 4 3 Printout index Basic parameters Optics Automation Printout Orientation le Quick Plot Portrait Full size plot Landscape Print layout Values Parameters Results Orientation Allow the way to print a graphic Portrait or Landscape Print Layout Allows choosing which types of data will be printed Values row data as absorbances Parameters the measurement parameters Results the calculation results as concentration activity 6 5 CHOICE OF ACCESSORIES gt lt Specific command for Uvi Light By choosing Parameters and Cell Changer the user can work with a cell changer By choosing Parameters and Sipper the user can work with an aspiration system and select the aspiration parameters See the use of this accessory at the end of this manual By choosing Parameters and Sample Changer the user can work with an external sample changer Specific command for UVIKON XL XS By choosing Parameters and Cell Changer the user can w
23. TRADE SECRET AND PROPERTY RIGHTS This document is protected by a SECOMAM copyright c 2003 and the copying rights are explicitly reserved The software supplied with the equipment or referenced contains trade secrets and confidential information which are SECOMAM s property It is legally protected by the international copyright c laws SCHOTT INSTRUMENTS grants a license to use its software to the user This may not be disclosed used or duplicated with the intention to save it without SCHOTT INSTRUMENTS s written permission The beneficiary must attach a copy of this document to all authorized partial or total reproductions 10 Lab Power software for Uvi Light amp UVIKON 3 INFORMATION The SCHOTT INSTRUMENTS equipment has been designed manufactured tested and inspected according to the ISO 9001 standards If the unit is not immediately installed it should be stored in a dry and clean area The storage temperature should be between 10 and 35 C SCHOTT INSTRUMENTS equipment is carefully inspected before it is packed As soon as you receive your equipment check the condition of the packaging and if you notice any problems notify your carrier within 48 hours Then consult the packing list and check that everything is in order Finally if you discover that something is missing or if the goods are damaged immediately notify SCHOTT INSTRUMENTS SCHOTT INSTRUMENTS Hattenbergstrasse 10 D 55122 Mainz Germany Tel 49 613
24. accessories board Switch on the spectrophotometer and run Lab Power software The sipper is automatically recognized during the initialization 15 2 2 Parameters selection Select Parameters and Sipper Sipper parameters X Sipper Yolume 0 2 40 ral Settling Time 1 20 s 1 E It displays cena Sipper Volume It is the sucking up volume 124 Lab Power software for Uvi Light amp UVIKON Settling Time Define the minimum time between the end of the sucking up and the sample measurement lt is also possible to use the Settling Time parameter which is located in the acquisition parameter of each measuring mode except Wavelength Scan Click on Execute and Fill Sipper or on the icon Ed to suck up the sample inside the flowthrough cell the measurement will start automatically after the end of time Settling Time When the sample is inside the flowthrough cell it is possible by clicking on the icon n to measure again the same sample The key amp allows getting back 90 of the sample Click on the icon Ed to suck up and measure a new sample 15 2 3 Function of sipper buttons Execute View Utilities Report Measure FZ Calculate F3 Break Clear Display Baseline Wavelength Galibration Fill Sipper AutoZero GoTo Wavelength Fi Instrument Switch Dr Instrument Switch Off E 22 60 0 0 fe ssi ASPIRATION AND M
25. lt S lt S CS S Type Wavelength scan psc Annuler En Give a name to the method i e test11 psc Click on Save 9 6 MEASUREMENT gt lt Measure made with a single cell holder See chapter 14 to use a cell changer Example Sample spectrum acquisition between 400 and 600 nm Specific command for Uvi Light Insert the blank solution in the single cell holder Click on Execute and Baseline or click on the icon Insert the sample Click on Execute and Measure or click on the icon M Measure samples one by one Specific command for UVIKON XL XS Insert blank solution and sample in the respective place in the single cell holder Click on Execute and Measure or click on the icon M Insert samples one by one It displays 56 Lab Power software for Uvi Light amp UVIKON kk x x a Ple Methods Parameters Execute View Uis Report Window Help ale fe aja H je uie 1 N A H a A u Il NO ml ies un Pardi E Pmi L parena eic T VER Gitana Abrabanel Dala Intereal fern 106 Gaan D GuchRangeY Anne StmSpeedemmel A Mo ot sangler I GbR 9D Creme a new document E a ed ee a IMPORTANT Not to forget in single cell mode to immediately save the curve before launching following measurement If the curve is not saved the new curves will take its place
26. menu select Parameters and Stirrer From the stirrer menu select the speed of the magnetic bars from 10 to 100 Select Off to stop the stirrer With the option Timed the stirrer works during 10 seconds with the last selected speed J LabPower Junior Gli ale Acquisition F8 Galculation Fa Cell Changer SIPBEF 127 Lab Power software for Uvi Light amp UVIKON 17 TEMPERATURE SENSOR lt 17 1 SPECIFIC COMMAND FOR UVIKON XS XL The temperature sensor is a miniature probe with an inert glass body to measure the temperature inside the cuvette A special support which is provided can be fixed in the cuvette compartment 17 1 1 Installation Switch off the spectrophotometer and connect the item cable on the connector of the accessories board Switch on the spectrophotometer and run Lab Power software The temperature sensor is automatically recognized during the initialization 17 1 2 Activation From the main menu select View and Temperature The temperature is then displayed on the screen V E z s re D Full Vier Absorbance Transmittance Graphics w Table Temperature Display Scale Label p Heading Toolbars Accessories 28 C Errors Fr 129 Lab Power software for Uvi Light amp UVIKON 18 THERMOSYSTEM lt 18 1 SPECIFIC COMMAND FOR UVIKON XS XL The THERMOSYSTEM
27. report CALIBO1 rep 13 2 CALIBRATION IN TIME DRIVE This measuring mode is not available on this software version 13 3 CALIBRATION IN AUTO RATE ASSAY This measuring mode is not available on this software version 110 Lab Power software for Uvi Light amp UVIKON num Options amp Accessories 111 Lab Power software for Uvi Light amp UVIKON 14 CELLCHANGER lt 14 1 SPECIFIC COMMAND FOR UVI LIGHT In this example each series comprising the measurement of two samples see the acquisition parameters it is not possible to make other operations Nevertheless the fact of clicking on the button Ey or making Execute and Break makes it possible to take again the hand WARNING It is not made the average of the results per series F LabPower Junior FIXED2 File Methods Parameters Execute View Utilities Report Window Djelsbelalalsl Fixed wavelengn miS Baal azco ela kd FIXED Measure Measured values Absorbance No Wavel Value 5 Value 5 Value 5 Value 5 1 580 00 0 3517 1 0 4981 0 2523 3 0 0480 4 14 1 1 Installation Switch off the spectrophotometer Connect the cell changer Install the cell changer Switch on the spectrophotometer and run Lab Power software see starting procedure paragraph 5 3 page 17 The cell changer is recognized at the time of initialization 14 1 2 Operating modes of the cell changer The reference blank is put
28. sample in the respective place in the single cell holder Click on Execute and Measure or click on the icon M Insert samples one by one It displays U LabiPower Junior OMECTCLE Les S fl wavelength Program ala a gt z oe ale xr caic ott 5 iss fa Mfferenct WB ouicrcu Measure MATES STI N Measured values z Alsorbange Caloulated values WLFundinn DiNerence Ho V uel Value 5 Cycle 1 Cycle 1 1 40500 1 6320_1 Sample 1 2 360 00 1 6730 1 D 1 2 0 0410 y Aequition Parametent jy Display amp Print Hol vaneen 3 EN nana Daisy si gg Cem 100 Average tens ls 10 Mo of sample 1 On the left are displayed the absorbances read against the reference at the 2 different wavelengths On the right the calculation results are displayed 92 Lab Power software for Uvi Light amp UVIKON 12 7 RESULTS BACKUP Click on File and Save as LabPower X Press YES to save calculated values Press NO to save measured values Click on Non to save the measured values here the absorbances Click on Oui to save the calculated values here the results the difference of the 2 absorbances read at 2 different wavelengths Example Test101 lpg for raw absorbance values read against the reference Results Ipg for the calculation results 93 Lab Power software for Uvi Light amp UVIKON 13
29. software for Uvi Light amp UVIKON 13 1 1 6 Backup of the calibration method O nomea F sales a Wavelength nm 580 0 Nominal Measured_S 10 000 20 000 40 000 80 000 Click on Methods and Save method or press F4 key LabPower Junior FWSTD2 E 18 x File Methods Parameters Execute View Utilities Report Window Help Wavelength nm 580 0 Nominal Measured S 10 000 20 000 40 000 80 000 Save method parameters i e CALIBO1 pcf 13 1 1 7 Standards measurement gt 4 Measure made with a single cell holder See chapter 14 to use a cell changer Specific command for Uvi Light Verify that the single cell is empty Click on Execute and Autozero or click on the icon AZ Insert the first standard Run the standard measurement by selecting Execute et Measure or by clicking on the icon Renew the operation to measure the other standards Specific command for UVIKON XL XS Insert blank solution and standard in the respective place in the single cell holder 98 Lab Power software for Uvi Light amp UVIKON Click on Execute and Measure or click on the icon M Renew the operation to measure the other standards LabPewer Junior FASTDI Ple Methods Paumetei Exec en Ubibes Report Window Dies 5 5 cotFixed Wavelength mic T ex e je mien Wavelengthinm 560 0 Hn Nominal
30. the following address SCHOTT INSTRUMENTS Hattenbergstrass 10 D 55122 Mainz We reserve the right to reship all instruments received collect failing such consent Whatever method and conditions of transport are chosen for the shipment of the equipment to be repaired under guarantee in the original packaging the corresponding costs and the insurance costs will be payable by the customer Any damage connected to the return transport of the equipment falls within the framework of the guarantee on the express condition that the customer has sent his complaint within forty eight hours by registered letter with acknowledgement of receipt to the carrier A copy of the letter should be sent to SCHOTT INSTRUMENTS For equipment with a guaranty card this is only applicable if the card delivered with the equipment is returned to SCHOTT INSTRUMENTS duly completed Lab Power software for Uvi Light amp UVIKON SOFTWARE GUARANTEE The software is guaranteed by the designer or the distributor of the software under the conditions specified in the literature accompanying the aforementioned software packages Under no circumstances whatsoever will SCHOTT INSTRUMENTS supply any type of guarantee for software packages By express agreement all damages notably for staff costs lost of earnings business trouble etc are strictly excluded from our guarantee The customer is informed that the software cannot be guaranteed exempt from defects or bugs
31. 1 66 5111 Fax 49 6131 66 5001 E mail support schottinstruments com 11 Lab Power software for Uvi Light amp UVIKON 4 HOW TO USE THE MANUAL This manual describes the operation of the Lab Power software applied to the Uvi Light XS XT XTD PC spectrophotometers and to UVIKON XS XL spectrophotometers It is consisted of the following parts Y Y Y ey PART I Startup Software installation Spectrophotometers connections Startup PART Il Common parts Description of software general functions PART IlI Measuring methods Analysis flow chart Description of different measuring modes PART IV Accessories Options and accessories As these apparatuses are different from each other they can comprise instructions of use different for each type of spectrophotometers We thus wrote each paragraph in the following way Paragraph name and number If this paragraph is followed by characters it is that it comprises particular instructions to one or more types of spectrophotometers They are located at the end of the paragraph In the absence of characters gt lt the instruction described in the paragraph applies in the same way to all the types of spectrophotometers A common part for all spectrophotometers This part has not a specific name A part if necessary comprising particular instructions to the spectrophotometers Uvi Light XS XT XTD PC The name of this part
32. 14 2 3 2 OSAMA een ee aa 116 14 2 3 3 6 samples 6 Te IGFeleeS ana a a I es 117 14 2 3 4 TO Sammples A e Sebasaan ie xusestcceaaas 118 14 2 3 5 Manualtoperalonea a n een ee eee ee ee ee eee ee ee eee eee 119 14 2 4 Cell changer use inside the various measuring modes eene 120 14 2 4 1 A ne a ne ae 120 14 2 4 2 Fixed wayeleneth 2er IM 120 14 2 4 3 IW ACIS TOU SCAU tl 120 14 2 4 4 Pe diN MEME 120 14 2 4 5 Wave lengri OOTAN T E E 120 15 SIEPER IP e N BES Rs RS 121 15 1 SPECIFIC COMMAND FOR UVILIGHT DD 121 LIA 3SIDPERSEIECH AA oe nn 12 A PPP 12 13 12 DIPPE PAra meters a pra acces 122 34 4 MOSUN EE EEE TETA ne 124 DA Backup of SIDDer parameters state E chess da sde E aio de ce ae 124 152 SPECIFIC COMMAND FOR UVIKON XL XS oooooccccccccnnnnnnnnnnnnnnnononananonnncccncnnnnnnnnnnnnnnnnnnos 124 Dd ISO EE ee er ee 124 LI 25 SPArameic s SCICCHON een ee 124 19 23 dJclonof SipDer DUTIODS nun ee ee 123 16 SEIRRER GL Pec 127 16 1 SPECIFIC COMMAND FOR UVIKON XS XL ooooccccccccccnnnnnnnnnnnnnnnnnnnnonornncccconnnnnnnnnnnnnnnnnnos 127 DOD Ts AAA m c H 127 10 1 2 ACUNA OW sd rait REI E Eee 127 17 TEMPERATURE SENSOR B 4 022020 282er 129 171 SPECIFIC COMMAND FOR UVIKON XS XL ooooocccccccconnnnnnnnnnnnononononononocccnononnnnnnnnnnnnnnnnos 129 TAE EE E0010 ais aaa 129 17122 ACAN ON ee ee usaba eese Putat t Bus 129 18 IHERMOSYSLIENM B iii mnhulelisnien
33. 2005 09 19 3 Uninstall Information S TESTWO1 D2 scn 1Ko Wavelength Scan Fi 22 02 2005 09 19 DO UY PRO v1 23 DO UY PRO v1 24 C3 Uv PRO v1 25 DO UY PRO v1 25b C3 Visicom Media C3 windows Journal Viewer windows Media Player C3 windows NT O WindowsUpdate O WinRAR O winzip C3 xerox c scoal O SPDATA O SYSTRANS C TELNET DO willy OG WINDOWS O WORK C3 Test Splitted Bee O WUTemp Sw Disque local D 5 Lecteur CD E W d sur 192 33 50 116 7 E E E E The operation proceeds in the same way when the user wants to split a multi curves file already saved In this case proceed in the following way Open the multi curves file 59 Lab Power software for Uvi Light amp UVIKON Select File Split or click on the icon El Select the main directory where the curves will be saved Click on Select The software creates a secondary directory bearing the name of the multi curves file followed by the mention splitted Inside this directory the curves are saved individually bearing each one the multi curves name incremented of an index 9 8 3 Cursor function The Cursor function is used to pick up quickly the values on a curve displayed on the screen When a measurement result is displayed on the s
34. 29 Repon SEU MO ups as se A Nemesis 107 13 1 2107 Repo Dacia een are coeptae ee ect er te unten 108 Peele A ee es a ea rest a a a tel 109 13 1 2 12 Summary ot the Backup teaser leute ei sine tos diete e 110 132 CALIBRATION TN TIME DRIV E 2 2 22 A a 110 13 3 CALIBRATION IN AUTO RATE ASSAY ccssssessscccseccssessscccssecnssessscccssecsseessscceseeeess 110 Lab Power software for Uvi Light amp UVIKON PART IV OPTIONS amp ACCESSORIES 111 14 CELL CHANGER PA at SA E MIN VET MENS 113 14 1 SPECIFIC COMMAND FOR UVI LIGHT occocnccconnnnnnnnncncnnnnononnnnnnnononononononoccncnnnnnnnnnnnnnnnnnnos 113 DED MEME em Ipse ERIT 113 14 1 2 Operating modes of the eell chanzer a Ha eps Bee a bea sein a tese eda sube qu 113 E IIIA DES 114 14 1 4 Cell changer use inside the various measuring modes sees 114 14 1 4 1 Fixed wayelenelh a uir emet oL ee SA 114 14 1 4 2 A eina RC E TIRE Po PES OR O TM 114 14 1 4 3 Time drive ai et REPOS Ha eO De c a Haie cab es M M C A AE ICI E 114 14 1 4 4 PAULO TALC ASSAY es Soc T S 114 14 1 4 5 Wave leni WhO Chains 2 ae stele d d Ma E E ML 115 14 2 SPECIFIC COMMAND FOR UVIKON XL XS eese enne Hee eene 115 LED APS O ee E O 115 JE H PP MES SIDE DETUR ee 115 14 2 3 Operating mode Of cell h ng o e e ao eoe ica coti acte ce ta eae comrade cond 115 14 2 3 1 Sample Offset Sarnen E AE 116
35. CE Calculation range 0 0 3 8 min sample 1 sample Sample 3 0 3676 0 3391 0 4408 ES 10 001 015 1 0114 0 9926 0 1831 0 1881 Acquisition Parameters MWweslenaglh ns E80 Dely af 06 Aeacings Aiming 20 05 Avemanguneli 10 Noosaperz 1 Graph Harga H 00 38 Display amp Print Pu Mode DET Graph Ranger Auterange l Mocomected On the left of the screen is displayed the curve of the reaction with the linear regression line On the right of the screen are displayed the number of the sample the activity slope of the straight regression line multiplied by the factor the standard deviation and the determination coefficient 11 7 RESULTS BACKUP Click on File and Save as LabPower E xl Press YES to save calculated values Press NO to save measured values A ee Click on Non to save the measured values here the curve Click on Oui to save the calculated values here the results the activity Example Curve aut for raw absorbance values versus time Results aut for the results activity standard deviation 81 Lab Power software for Uvi Light amp UVIKON 11 8 CURVES OPERATIONS The following functions Curves merging Splitting of a multi curves file in individual files Cursor function Zoom function Absorbance Transmittance function Graphic Table function
36. EASUREMENT OF THE SAMPLE Select Execute and Fill Sipper or click on the icon Pitt The sample will be moved inside the flowthrough cell and the measurement will start automatically after the time Settling Time If the user click on the icon M in place of the icon E the sample inside the flowthrough cell will be measured again without waiting time RINSING 125 Lab Power software for Uvi Light amp UVIKON Select Execute and Sipper Rinse or click on the icon This function is used to rinse the sipper The peristaltic pump works all the time that the operator pushes the key RECOVERY OF THE SAMPLE Select Execute and Sipper Return or click on the icon amp This function is used to get back 9096 of measured sample 126 Lab Power software for Uvi Light amp UVIKON 16 STIRRER 16 1 SPECIFIC COMMAND FOR UVIKON XS XL This accessory is made to be installed inside the base of the standard single cell holder The stirrer consists of two engines with D C current which controls small permanent magnets These magnets involve the rotation of magnetic bars in the tanks 16 1 1 Installation Switch off the spectrophotometer and connect the item cable on the connector of the accessories board Switch on the spectrophotometer and run Lab Power software The stirrer is automatically recognized during the initialization 16 1 2 Activation From the main
37. EINGAMEASUREMENT n eek Ot DOING CALCULA TON ee Reel 68 DATA DISPLAYING ares ar ee ee 6 DATA oe RU uu 6 10 DATA TIERE OPENING cL cm 6 11 PA ee et ee ee ee d uae Od AS I qoa Io propa o UR TR odlas EXCEL AITO Few rc e TT ERNST Oei A rcc E OI BUMA DIPIT OO tautum utate fm ita OLI Der DOW AV GS CLO OCIS sissies giao scala tea trie Wie ccc 6 12 USERS METHODS pnl iii A A BAC A 0 42 D ov oad E NERO 6 13 STOP OEMEASUREMENT neue 6 14 DELETING OF DISPLAYING DATA 222200uussesnessnsnnnneennnnensnnnunennnnnennnonnnennnnsnnnnenuneenneen 6 15 CALCULATOR FUNCTION see ee ee 6 16 REPORT EDIT see Dee Eee 6 17 PRINTING OF THE RESULTS ae ee Lab Power software for Uvi Light amp UVIKON PART HI MEASURING METHOD 39 Tr PLOW SHART sa SA E A AAA 41 8 FIXED WAVELENGTH METHOD ococ cioniniosionicinnionecconinnenannion ncanionccdanccnconanicnvivaninccadani ccnine 43 S L CHOICE OFTHE METHODE erer RE ee ee 43 8 2 CHOICE OF ACQUISITION PARAMETERS eeeeeete eene ennt nana annee E sess ona na ranos 43 Sos JEIDEOPENINGLss itor testis cauudosietL ee ee ee 44 84 CHOICE OF CALCULATION PARAMETERS 5 0220 44 8 4 1 WTC ATC OF CSS OM dia 45 8 4 2 UAT OLORES O ee A ee ee ER Dee 45 8 4 3 ID Ola OT nee es re ee ee Sead cia ee ee 46 8 4 4 CONCENTRA A A A A E aaa 46 8 4 5 LUNU ONS n RT 47 Boe VE EHO AC RUP ee MA i 47 5207 MEASUREMENT uo P
38. Following example FIXED1 Fixepi px MB a Ela Az Go 6 13 STOP OF MEASUREMENT To stop the measurements click on Ctrl Alt or on the icon Ee 35 Lab Power software for Uvi Light amp UVIKON All the operations of acquisition calculation screen actualization entry of parameter loading safeguard conversion or impression will be stopped The data measured up to this point are valid and can be stored and used for a later reprocessing AN WARNING Leav e a method without saving the data does not allow finding them A message informs of this situation 6 14 DELETING OF DISPLAYING DATA To erase the data displayed with the screen click on Execute and Clear Display If the data had not been previously saved the user is informed by it by a warning message 6 15 CALCULATOR FUNCTION The standard program Calculator of Windows can be carried out inside the main program by selecting the menu Utilities and Calculator or by clicking on the icon in the Calculation tools bar 36 Lab Power software for Uvi Light amp UVIKON 6 16 REPORT EDITING It is possible to create an ASCII file containing the results of measurements as well as comments or any other information which the user will wish to add to it This file could be imported by a text processing to generate a personalized report which will be able to also contain the graphs exported with format BMP When al
39. If the user saves the previous curve it is the new curve which will not be maintained in memory 9 7 RESULTS BACKUP Click on File and Save as ll MM MM ME Mm MM MM ME MM ME MM MM MM MM MM MM ME MM ME MM mM mM m m Save ie C BHO C test1WW Splitted 400500 425475 pr2 YELLOW GROUP testi Data WLSCAN sen Give a name to the curve i e Curve01 scn Click on Save to save the curve 57 Lab Power software for Uvi Light amp UVIKON 9 8 CURVES OPERATIONS 9 8 1 Curves merging It is possible to merge curves with other previously saved Click on Open to download the first curve Id Click on File and Merge or click on the icon The following dialog box appears Open xl Ei testaww ES wscan E YELLOW ES SAMARIUM It is the curves list of wavelength scan method Select the curve to merge Click on Open If the selected file includes only one curve the following dialog box appears Charger un chanti x Iv Echantillan 1 T l 2 UF S lectionner les amp chantillons charger en activant l onglet et crire le nouveau num ro d chantillon dans la bofte d dition amp nnuler Give to the curve a different number from that of the already displayed curve NOTE It is possible to select several curves to merge by selecting all the files mouse left click
40. L deriv SCNMesure ATEM E 9 8 7 3 X all Samples displaying Allows the user to redefine the scale of the axis X Abscissa of all the curves displayed in the main window 63 Lab Power software for Uvi Light amp UVIKON i Graph range selection xj Range CY all Samples Low 190 1099 nm Autorange all Samples High 131 1100 nm C Y variable Lancel Example of displaying X all Samples with X Low 400 nm and X High 500 nm W uot deriv SCNM 9 8 7 4 Y variable displaying Allows the user independently to define for each displayed curve the Y axis scale i Graph range selection Range C Y all Samples C Autorange C X al Samples Y variable Samples with different values Set Cancel Click on Set It displays Variable Y values Auto lt al x Y min Auto Sample 1 jo m n m Sample 2 ja m Fr r Sample 3 a o m Sample 4 fo mi E m Sample 5 o mi om Iv Sample 5 oO mi fa Iv Sample 7 mi zm m Sample 8 jp mi m Sample 9 pp Im m Sample 10 D Imi md m caen Deselect the box Auto of curves which one wants to change the Y scale Enter for each curve Y mini and Y maxi 64 Lab Power software for Uvi Light amp UVIKON NOTE It is possible to let the software choose the minimum or the maximum value of the Y scale for each sele
41. LAB POWER software for Uvi Light amp UVIKON XL XS User s manual Manual version 1 0 Software version 3 20 SCHOTT Instruments Lab Power software for Uvi Light amp UVIKON SUMMARY 1 REMARK S tar IRRE AAA AE e WARRANTY aaa TNEOR MA O ern oe mM HOW TO USE THE MANUAL uta linde PARTI START UP 15 S INSTRUCTIONS OF USE u a einen 5 1 MINIMUM CONFIGURATION RECOMMANDBED seen eene nnne nennen nnns 5 2 SOFTWARE INSTALLATION FROM THE CD ROM cccccsccssscccsccceeeeeesseesesssnseeeeeeeseeeeeees 5 3 SPECTROPHOTOMETERS CONNECTION Dd een 3 4 OVE Tal GT TONGS sect assis te haa a yaad eee aie sae hate oat ie aha eed ite ts alia tn ol 332 OFROHET BO ae 2 323 UPELIGETAT ANDOCEDIPBSION ud an thai no 5 3 4 CVIK ON ATAS tds Se INEA LIZA TON id 5 4 1 AA ee ee 5 4 2 SYSTEM QUTOLESt and DU OH ZO LOL ee 3 4 3 SOPPI Of THO A acis een 5 4 4 A aie Pat D E A E PART II COMMON FUNCTIONS 23 6 GENERAL FUNETIONS ass IND SIRVE SEN SR II bAU EDS EE D I EI SL DISPLAY SCREEN pec 6 2 CHOICE OF ACQUISITION PARAMETERS cccccccsssesessceeeeeessesesccesecesssecescseeedeeesaceseaeeeees 6 3 CHOICE OF CALCULATION PARAMETERS cccccccssssssscceessessscesscceecsessscessecerseessecensecenees 6 4 CHOICE OFB ASIEPARAMETERSA EEE aaa ala 6 4 1 CODES in MN HR 6 4 2 CAM ONAN 10 0 CON REC E A 6 4 3 0 21710017 125 INA ee ct CT 6 5 CHOICE OF ACCESSORIES PA as 6 6 STEAR
42. Select Parameters Acquisition and Set Enter the wavelength as follows 87 Lab Power software for Uvi Light amp UVIKON Wavelength value selection Wavelength Wavelength 1 nm 405 Wavelength 2 nm 360 Wavelength alnm 340 Cancel Select Parameters and Calculation Calculation Parameters Wavelength Program l xX r Calculation i Off C Base Point C Functions Wavelength Function Functian Concentra WL Fairs Ma af Calculations E WIL Factors caen Select Concentration for Function Enter 2 for No of Calculation Click on WL Pairs 88 Lab Power software for Uvi Light amp UVIKON Wavelength Pairs Wavelength I nm Wavelength 2 nm Wavelength Pair 1 405 0 m 360 0 Wavelength Pair 2 i 405 0 3 240 0 Lancel View Wavelengths Enter a number here between 1 and 3 which corresponds to the order of WL choice Validate Click on Wavelength Factors Wavelength Pairs Factors ED Factor 1 Factor 2 Wavelength Pair 1 2 4 Io 3 Wavelength Pair 2 2 4 0 34 Lancel Enter the factors and validate Valid the whole of parameters Run the measurement The results will be displayed as follows r Junior ONECYCLE E 181x File Methods Parameters Execute View Utilities Report Window Help D c 5 l BS Wavelength Pr
43. T 48 5c RESULTS BACKUP tc ac 49 9 WAVELENGTH SCAN ae 51 9b CHOICE ORFTHE METHOD 2 a RE EI un Rare 51 9 2 CHOICE OF ACQUISITION PARAME TERS con ei pea rhv ah xr epa ee etn Rlecdeaens Rites 51 DO TILL OPENING ni A A lado 22 94 CHOICE OF CALCULATION PARAMETERS o occccccccccconccononocinococonicocinoconococonecoconocenecaconicncinenins 52 9 4 1 PONER ne es seen 53 9 4 2 Curve DDERUTLONS wen nes ge seen ir Sees nee ae 54 9 4 3 BUNCHONS A Reese ee 54 9 4 4 PD CTV QTY sense Reese ae 39 9 4 5 SMOOL NND ze TCR 53 Bo SMELHODBACKR UP nee A AT UcE E Uf 56 9 0 MEASUREMENT On ee cite 56 Du RESULTS BACK Pala ee Nee 57 28 CURVES OPERATIONS zn ee nen Neger 58 9 8 1 CUT VCS AS A ee es ee 58 9 8 2 SELES Of TNL CUT VCR TU CS EE 59 9 8 3 QR ETAT SITIS sit ds 60 9 8 4 ZOOM TUI RT 60 9 8 5 Absorbance Transmittance function eese enhn enhn nnnennnn senes nsns sss s isses sensns 61 9 8 6 Grapes able TUACIONS nella 61 9 8 7 DIL eT AURA O A ern 62 98 1 1 Autoranbe o ee ne seele teen 62 Dll Y all Samples dISplayIO Dreta 63 1013 Call Samples display e id een DO O al iad 63 9 8 74 A 64 9 8 8 Label TURCO AA SAA A AAA ee 65 9 8 9 CH AOM E JUNCTION TE 66 98 10 Display Samples UICN A Ian 66 OSTI Baseline ranged TJUHCHON anna Bau 67 IU TIMEDRUIVE Uu ceto inei a ei anal 69 10 1 CHOICE OFT PEE METHODE ei
44. W SAMARIUM Calculations E admi xdi Measured values Absorbance No Wavel Value 1 500 00 0 0647 1 499 50 0 0674 1 2 3 499 00 0 0681 1 4 498 50 0 0666 1 5 498 00 0 0637 1 6 497 50 0 0607 1 7 497 00 0 0578_1 8 496 50 0 0558_1 9 496 00 0 0542_1 10 495 50 0 0528_1 11 495 00 0 0519_1 12 494 50 0 0516_1 13 494 00 0 0523_1 14 493 50 0 0531_1 15 493 00 0 0553_1 16 492 50 0 0579_1 17 492 00 0 0611_1 18 491 50 0 0651_1 19 491 00 0 0703_1 20 490 50 0 0769 1 21 A49n nn fn n844 1 zl 9 8 7 Display Scale function This function allows modifying the curves scales displayed in the main window Select View and Display Scale 9 8 7 1 Autorange displaying The software chooses automatic the scales of axes X and Y in such way that all the curves hold in the principal window The scales correspond roughly to the largest curve Graph range selection X Range CY all Sample Autorange C X all Samples C Y variable Cancel 62 Lab Power software for Uvi Light amp UVIKON Example of displaying Autorange WHOL deriv SCNMesure O x 9 8 7 2 Y all Samples displaying Allows the user to redefine the scale of the axis Y Ordered of all the curves displayed in the main window f Y all Samples Y Low 9999 9999 o C Autorange CY variable Example of displaying Y all Samples with Y Low 0 Abs and Y High 2 0 Abs WHO
45. ading FIXED WAVELENGTH Select Report and Insert Insert in the desired order Y LabPower Junior CALIBO1 2 Report X File Methods Parameters Execute View Utilities Report Window Help la x D fc e Fixed Wavelength mjg AAEE E Az Go ajaj 4 gi Lin Reg Hd y Ax B CALIBO1 Analysis Report Date Thursday August 03 2006 Operator Heading FIXED WAVELENGTH PARAMETERS WAVELENGTH nm 580 0 DELAY BI 0 0 AVERAGING TIME s 10 NO OF SAMPLES 1 CALC MODE Lin Reg Reference data CALIBO1 uns aa EE off LAMP CHANGE nm 338 BANDWIDTH nm 18 PAT OP HON PA Off AUTOSAVE Off the parameters F LabPower Junior CALIBO1 2 Report IN File Methods Parameters Execute View Utlities Report Window rrr plss Fixed Wavelength m z E e E AZ Go n a CALIBO1 File mode FIXED WAVELENGTH Sample No of Measurements Mode Calculation Errors Date Time 1 2 AU Lin Reg 25 02 2000 10 03 the file info Y LabPower Junior CALIBO1 2 Report X File Methods Parameters Execute View Utilities Report Window DIESELBE rea Warsiengn EA el Fr lala t aos nje CALIBOD Measured values Absorbance No Wavel Value S 1 580 00 1 4482 1 2 580 00 1 4482 1 the measured values Y LabPower Junior CALIBO1 2 Report NN F
46. alue from the absorbance values Allow to multiply or to divide the absorbance values by a constant value Allow to calculate the logarithm of the absorbance values Enter the function Enter the constant value Click on Close The calculation selected in Calculation Parameters will be executed on the absorbance values by clicking on Execute and Calculate or by pressing F3 key or by clicking on the Mc ICON 8 5 METHOD BACKUP J LabPower Junior FIXED2 X FIXED2 Measure a IB x Click on Methods and Save method or press F4 key Enregistrer sous w 9 Enregistrer dans C5 WORK E er FA PA FINED pes rd Test03 pf Ooo o a Type Longueur d onde fixe pf Annuler E Give a name to the method i e test04 pfx Click on Save 47 Lab Power software for Uvi Light amp UVIKON 8 6 MEASUREMENT lt Measure made with a single cell holder See chapter 14 to use a cell changer Example Samples measurement at 580 nm using a factor of 2 5 Specific command for Uvi Light Insert the blank solution in the single cell holder Click on Execute and AutoZero or click on the icon AZ Insert the first sample Click on Execute and Measure or click on the icon M Insert samples one by one Specific command for UVIKON XL XS Insert blank solution
47. ance X Concentration Enter values of coefficients A B and C if they are known or choose a calibration curve taking again this calculation by clicking on the button Load and by choosing the file comprising this curve Choose the units Click on Close 45 Lab Power software for Uvi Light amp UVIKON 8 4 3 Interpolation Calculation Parameters Fixed Wavelength Values Reference CALCURYVE Calculation Dif Linear Reg Units C Quadratic Rear Interpolation C Concentration C Functions ned Choose a calibration curve taking again this calculation by clicking on the button Load and by choosing the file comprising this curve Choose the units Click on Close 8 4 4 Concentration Calculation Parameters Fixed Wavelength Calculation Values Reference CALCURVE C Off C Linear Reg Units C Quadratic Regr Interpolation Conc Factor 26 176723 Concentration C Functions Calculation CONCzg ABSg x Conc Factor Enter the concentration factor Choose the units Click on Close 46 Lab Power software for Uvi Light amp UVIKON 8 4 5 Functions Calculation Parameters Fixed Wavelength c Off Linear Reg Function aperatian C Quadratic Regr Function Constant Interpolation C Concentration Functions z Allow to add or to subtract a constant v
48. and sample in the respective place in the single cell holder Click on Execute and Measure or click on the icon M Insert samples one by one It displays LabPower Junior TESTCONC E 81 xl File Methods Parameters Execute View Utilities Report Window Help EEE Fixed Wavelength miz J EA E Az Go ajaj a Zi coneractor sz zle es CALCURVE Ed TESTCONC 1 Calculations Calculated values Conc Factor Calculated values Conc Factor No Wavel Value No Wavel Value 1 580 00 0 5150_1 1 580 00 1 2875 2 580 00 0 5150_1 2 580 00 1 2876 3 580 00 1 9338_1 3 580 00 4 8345 Acquisition Parameters Display amp Print Spectrophotometer informations Wavelength nm 580 0 Print Mode vales Wavelength nm Delay s 0 0 Averaging time s 1 0 No of samples 1 Not connected In the left window there are the absorbance raw values In the right window there are the concentration values absorbance x factor here 2 5 48 Lab Power software for Uvi Light amp UVIKON 8 7 RESULTS BACKUP Click on File and Save as LabPower X Press YES to save calculated values Press NO to save measured values ma XE X Click on Non to save the measured values here raw absorbance values Click on Oui to save the calculated values here the concentrations Example ABS fix fo
49. by selecting Parameters and Cell Changer e Methods fee Execute View Utilities Report ww Mass Acquisition F8 SA relation ES ll fea Basic Cell Changer Ort Shiner k X BS amples Ref SIPPE BS amplesbRet 105 amplesZRef Sample Offsets 115 Lab Power software for Uvi Light amp UVIKON 14 2 3 1 Sample Offsets It is possible to enter separately an offset for each sample except for the reference The data are displayed with the offset directly deducted for each sample The possibility of deducting an offset can be very useful when the analyzed samples have different levels of absorbance 14 2 3 2 6 samples 1 reference This mode allows measurement of 6 samples in the sample cell holder against 1 reference in position 1 of the reference cell holder Only the sample cell holder moves during measurement Figure below shows the cell holder positions at start and end of a measurement if all 6 cells are measured Measurement start a Measurement end Reference beam uar Reference beam A Sample beam l Sample beam x n d This mode can also be selected starting from the dialog box of the manual use of the cell changer Lab Power software for Uvi Light amp UVIKON 14 2 3 3 6 samples 6 references This mode allows measurement of 6 samples in the sample cell holder against 6 references in the reference cell holder Both holders move simultaneously durin
50. by this spreadsheet Choose File Export and Excel Choose the directory give a name to the file and make Save 6 11 3 UV PRO format The spectra stored on the disc can be converted in UV PRO format to be used by this software Choose File Export and UVPro Choose the spectrum to be converted Choose a destination folder by default WORK with the icon The user can save directly his spectrum in the folder where is the UV PRO software Click on Select to validate the destination folder Dilutian ratio Te i Optical path mm 10 OF Cancel Enter the dilution ratio 1 1 no dilution 1 2 1 volume of sample for 2 total volumes Enter the optical path of the cuvette Enter if required information concerning the sample Click on OK The spectrum will be exported in the folder thus indicated with the extension scn 33 Lab Power software for Uvi Light amp UVIKON NOTE the software adds in the name of the spectrum the suffix UV Pro to avoid any confusion between a raw spectrum acquired by the software Lab Power and this same spectrum converted with the format UV PRO and which could be possibly found in the same folder It adds also a sequence number The user can decide to export a file spectrum gathering several spectra maximum 10 During conversion the file is burst in several files 1 per spectr
51. c 2I bl Time Drive m z aja alal El AZ Gol ajaj Acquisition Parameters Wavelength nm 580 0 Edit Delay s 0 0 SamplngRate mi 60 Measure Time min 10 0 No of samples 1 Display amp Print Spectrophotometer informations Print Mode Values Wavelength nm Absorbance AU Graph Range Y Autorange Graph Range X A default file will be open and its name will be TDRIV1 Measure Not connected ff 10 4 CHOICE OF CALCULATION PARAMETERS Click on Parameters and Calculation 70 Lab Power software for Uvi Light amp UVIKON Calculation Parameters Time x Values r Calculation v pfi C Peak Detection C Cure Operations Activity C Functions C Derivative C Smoothing Close Cancel Several types of calculation can be applied to the values of reading absorbance to calculate the concentrations Peak Detection Curve Operations Activity Functions Derivative Smoothing 10 4 1 Peak detection See the same function in chapter WAVELENGTH SCAN paragraph 9 4 1 10 4 2 Curve operations See the same function in chapter WAVELENGTH SCAN paragraph 9 4 2 10 4 3 Activity This function allows calculating an enzymatic activity using the linear regression method Activity Slope of the regression line calculated for one minute x Factor 71 Lab Power software for Uvi Light amp UVIKON Values DH Refere
52. ch has been manually selected The manually used of the cell changer is activated by selecting View Accessories LL and Cell Changer Manual Control or by clicking on the icon luz euro Zoom Fal Sane Aso ence Irarenittange Graphics able Temperature Diplan seale Label F Heading Display Samples Baseline range Toolbars k Sippet Cell Changer Manual Control Emoe E IHer meanstem Lell Changer NOTE The cell changer keys S and R can be used to manually change the cells in the sample and reference beams The cell changer keys can be used even when a cell changer mode has been selected In this case both cell holders are moved automatically to position 1 at measurement start 119 Lab Power software for Uvi Light amp UVIKON 14 2 4 Cell changer use inside the various measuring modes 14 2 4 1 Auto rate assay In this method the instrument can measure up to ten samples simultaneously when the cell changer is in use Measurement starts with the first reading on sample 1 The cell changer then moves to the next position and measures the first reading on sample 2 This process continues until all selected samples parameter No of samples have been measured once The instrument will then continue measurement with the second reading on sample 1 and all other samples The whole process is repeated until the selected number of readings has been measured Up
53. con Ey inside the main tools bar All the measurements taken until this moment are valid and can be stored and used for secondary treatments 6 7 DOING A CALCULATION mo AM To click on the icon i or to do Execute and Calculate allows running a calculation on one to ten samples whatever the selected method Only parameters from the calculation and display columns of acquisition parameters are used for the calculation The calculation can be stopped at every time by clicking on Ctrl Alt or on the icon i of the main tools bar NOTE The calculation parameters remain active as long as the user does not have selected Calc Off in the menu a aon That means that same calculation will be carried out with each time a measurement is made 6 8 DATA DISPLAYING This command is used to bring up to date the display of the principal window and the window of results on the screen u By clicking on View and Graphics or on the icon E the graphic is displayed 31 Lab Power software for Uvi Light amp UVIKON By clicking on View and Table or on the icon El the graphic values are displayed 6 9 DATA BACKUP The measurement results can be saved on the hard disk by clicking on File and Save or by pressing Ctrl S or by clicking on File then Save as The following dialog box appears 21x Enregistrer dans 5 Work de t E EE MEASP 7 testiWW
54. consists of a quick lock automatic 6 6 Cell Changer with a Peltier effect thermostating system and magnetic stirring capability The THERMOSYSTEM must be connected to an external liquid circuit to cool the Peltier elements 18 1 1 Activation From the main menu select View Temperature and Thermosystem or click on the icon IET Utilities Report window Edea R miss sz sii mee BUSE Gaia ee Iraremittanee Graphics lebe Temperature Penam scale Label F Headimar Display Samples Baseline range Toolbars k SIPPE Cell Changer Manual Control T hermoS vstem Ermo FG 131 Lab Power software for Uvi Light amp UVIKON 18 1 2 THERMOSYSTEM displaying When the THERMOSYSTEM is activated the following screen is displayed Thermo System Sample JE Reference C T aj C 10 045 25 035 a E min a L min 10 05r 104 Stirring Min __ Max By clicking on this button al Top right the screen will be in reduced mode See under Thermo System Reference C Then only temperatures are displayed 18 1 3 Remote control or local mode The THERMOSYSTEM can be used in local mode with the built in keyboard of the control box or in Remote control mode through Lab Power software Remote control mode local mode 18 1 4 THERMOSYSTEM functions Temperature programming The tempera
55. creen like a curve select View and Cursor Move the cursor on the screen it has the shape of cross and click on the mouse left button when it is placed at the place of the curve which one wants to know the value It is also possible to use the arrows of the keyboard The values for all the curves appear in a box associated with the color with the curves on the graph a vertical line materializes the movements of the mouse X 447 50 valeurs de ini xi Y 121 6641 Y 223 3281 Y 320 4150 The text in the window associated with the graph indicates the position of the cursor X and the value of the curve to this point Y1 One can leave the mode cursor while again selecting View and Cursor or more simply by pressing the key Esc or the right hand side button of the mouse 9 8 4 Zoom function The function Zoom is used to increase a particular zone of a graph Select View and Zoom or click on the icon El Move the cursor on the graph and place it on the left upper corner of the zone to be increase Press the mouse left button and move it without release the button One sees a rectangle moving on the screen which represents the area which will be increased Reach the right lower corner of the desired area and release the button Selected surface is increased to occupy the entire graphic zone 60 Lab Power software for Uvi Light amp UVIKON Acauigtion Parame
56. cted curve The operator selects at this time the corresponding box Auto Example of displaying Y Variable with Sample 1 Y min 0 and Y max 2 5 Sample 2 Y min 0 5 and Y max 0 5 Mio deriv SCNMesure o Se ARA RRE 9 8 8 Label function It is possible to add a comment to any place of a graph to describe the experiment Select View Label and Add when the curve is displaying The following dialog box appears Add Label x Content SAMARIUM spcetrun Cancel Enter the text made up of alphanumeric characters and click on OK A rectangle defining dimensions of the entered text will appear on the graph and will follow displacements of the cursor Position it at the desired place and press the mouse left button If the rectangle is entirely contained in the graphic zone the entered text will be displayed F SAMARIUM Calculations 65 Lab Power software for Uvi Light amp UVIKON To delete a comment click twice on the comment zone with the mouse left button A rectangle surrounding the comment zone will appear Select View Label and Clear to delete the comment All comments can be deleted by selection View Label and Clear All The added comments are saved with the data file and so can be called back with data 9 8 9 Heading function This function makes it possible to add a heading d
57. d in the column Nominal Click on Close The curve is redrawn by taking account of the standard which was removed Check if 0 000 Abs is always displayed on the screen if not do again the zero Uvi Light spectrophotometer Insert in single cell holder the standard to be measured again Run the standard measurement Me Display again the curve by selecting View and Graphic or by clicking on E 102 Lab Power software for Uvi Light amp UVIKON bPower Jumi This one takes account of the new measured value of the standard Select File and Save as to save the new calibration curve 13 1 2 Unknown samples measurement 13 1 2 1 Choice of the method Select Methods New Method and Fixed Wavelength 13 1 2 2 Choice of acquisition parameters Parameters Fixed Wavelength Acquisition Wavelength 190 0 1100 0 nm E Delay time 0 0 3000 0 s 20 Averaging time 0 1 60 0 s Number of samples 1 6 cos Select Parameters and Acquisition Enter same parameters as for the calibration curve Click on Close 13 1 2 3 File opening Select File and New 103 Lab Power software for Uvi Light amp UVIKON kd faros measure 13 1 2 4 Choice of calculation parameters Select Parameters and Calculation F LabPower Jun or FIXELKZ
58. ds Parameters Execute View Utilities Report Window Help DEE Autorate Assay m z aa alal El Az Go ajaj E ARATE1 Measure Acquisition Parameters Wavelength nm 580 0 Delay s 00 Readings N min 20 0 5 Display amp Print Spectrophotometer informations Print Mode Values Wavelength nm Absorbance AU Graph Range Y Autorange Graph Range X A default file will be open and its name will be ARATE1 Measure Averaging time sk 1 0 No of samples 1 Not connected fff 11 4 CHOICE OF CALCULATION PARAMETERS Click on Parameters and Calculation 78 Lab Power software for Uvi Light amp UVIKON Calculation Parameters Auto Rate Assay Values C Peak Detection C Activity C Functions C Derivative C Smoothing Ensfit caen Several types of calculation can be applied to the values of reading absorbance to calculate the concentrations Peak Detection Activity Functions Derivative omoothing 11 4 1 Peak detection See the same function in chapter WAVELENGTH SCAN paragraph 9 4 1 11 4 2 Activity See the same function in chapter TIME DRIVE paragraph 10 4 3 11 4 3 Functions See the same function in chapter WAVELENGTH SCAN paragraph 9 4 3 11 4 4 Derivative See the same function in chapter WAVELENGTH SCAN paragraph 9 4 4 11 4 5 Smoothing See the same function in chapter WAVELENGTH SCAN paragraph Feh
59. e operator started measurement and the moment where the real measurement will be carried out Average time Defines the time in second used by the unit to gather the data and to calculate an average value on a reading During measurements with strong absorbance to reduce the noise it is recommended to use a high value No of cycles It is the number of times that the measurement of spectrum will be repeated on the same sample maximum 10 Cycle time It is the time of a cycle expressed in minutes This time includes time necessary to measurement Number of samples Fix the number of samples to be measured per series lf a cell changer is used the cuvettes are measured automatically 12 3 FILE OPENING Click on File and New It displays J LabPower Junior WPROG1 File Methods Parameters Execute View Utilities Report Window Help Dy cs Wavelength Program mgl fea lala E AZ Gol n z MN wrroG1 Measure Acquisition Parameters No of wavelengths 3 Display amp Print Spectrophotometer informations Print Mode Values EN Delay s 00 Cycles N min 1 0 0 Averaging time sk 1 0 No of samples Not connected f l 1 A default file will be open and its name will be WPROG1 Measure 12 4 CHOICE OF CALCULATION PARAMETERS Click on Parameters and Calculation 84 Lab Power software for Uvi Light amp UVIKON Calculat
60. ed and smaller is the sucked up volume When the remaining volume in the tube is equal to the volume of the standard tube click on OK 15 1 3 Sipper parameters x E azl To Select Parameters Sipper and Sipper parameters 122 Lab Power software for Uvi Light amp UVIKON z Sipper Volume 0 2 40 ml 2 Lack Time 1 20 s fin Dead Yolume O 2ml 0 1 ET coe Sipper Volume It is the sample volume expressed in pl Enter one value between 0 2 and 40 ml The recommended value for a smoothing outputs flowthrough cell is 700 ul Lag Time It is the time expressed in seconds between the end of sucking up and the beginning of measurement This time makes it possible the sample to degas bubbles or to be stabilized in the cuvette watered effect Enter a value between 1 and 20 seconds Recommended value 4 seconds Dead Volume This dead volume will be sucked up between each sample in order to separate them and to reduce the contamination inter samples Enter a value between 0 and 2 ml Make sure that the beginning of dead volume end of the sample is always outside of the flowthrough cell see the drawing below This dead volume depends on the length of the aspiration tube The more important dead volume will be the more the end of the volume of the sample will be close to the input of the flowthrough cell Fig 1 Dead volume explanation
61. ement entscheide 85 DLA DS RAULO MEOS UF ENE syn 87 12 4 3 CONCENTRA LION Measurement u essen 87 12 4 4 MBAS CMOS Cts a oran 90 12 423 LUNU ONS USO AAA AA AAA ASA A 90 12 9 METHOD BACKUP re A AAA R 91 12 6 MEASUREMENT Dl are 91 12 7 RESULTS BACKUP iii aa een tuto a NN co aU OS 93 13 CALIBRATION zes Eve A RV A EE RYE EIN ata 95 13 1 CALIBRATION IN FIXED WAVELENGTH 42 222 222 a SU eese 95 TO a CAUDA ON CUNY Cy seen nee Reese las 95 1321 12 Choles oF theme 0106 a ae el ee ee ee eee 95 13 1 1 2 Choice o tacquisition parameter tata nennen ala 95 13 1 1 3 A a rd en a 96 13 1 1 4 C hoice ot calculatioOn parameters state to la e e tl o e dd hn 97 13 1 1 5 Choice of calculation THOOO nun ouea nes ea bed es a ener 97 13 1 1 6 Backup OF the calibration method tte 98 13 1 17 Standards measurement Pt oett I tee eet tete He eH Feci eire 98 13 1 1 8 Displaying of calibration CUEVE en ee nee nee 99 13 1 1 9 Backup of the ealibration UV pp t aet qe e TR S 100 TARLA DEl OF a A Om e e eye 101 13 1 2 Unknown samples Measurement en 103 PARAL CHOICE OL Mille Nonae een cu MUR C r I 103 13 1 22 CHOIC COL ACGUISINON Parametern ae E bs 103 13 1 2 3 A A esse 103 13 1 2 4 Choice Of calculation Daramielets area ea a a a a oes dn catus 104 13 1 2 5 Method Dick asia its 105 13 1 2 6 Samples measurement P dt ee 106 13 1 2 7 Calculation of the samples concentration 2 eu 106 13 1 2 8 Results Backup nee euer 106 1341
62. g measurement see figure below Measurement start Reference beam E Reference beam A Sample beam Sr Sample beam This mode can also be selected starting from the dialog box of the manual use of the cell changer Lell Changer 117 Lab Power software for Uvi Light amp UVIKON 14 2 3 4 10 samples 2 references This mode allows measurement of 10 samples 5 in the sample cell holder and 5 in the reference cell holder against 2 references ref 1 in position 1 of the ref cell holder ref 2 in position 6 of the sample cell holder Both holders move independently during measurement see figure below Measurement start Beam switching Measurement end If more than 5 samples are selected in the 10 samples against 2 references mode the sample and reference beams are swapped after the fifth sample has been measured This unique Beam switching is possible due to the fact that both beams have a fully symmetric optical layout as well as symmetrical data acquisition electronics This mode can also be selected starting from the dialog box of the manual use of the cell changer Cell Changer 118 Lab Power software for Uvi Light amp UVIKON 14 2 3 5 Manual operation If the cell changer Off mode is selected it can be manually moved by using keys S Sample and R Reference on the keyboard below Lell Changer se mm LL EE LLa LL br lr The measurement is done on the cell whi
63. ge Insert the software CD ROM in the CD ROM reader The installation of Lab Power starts automatically Follow the instructions displaying on the screen The Lab Power software will settle automatically according to the version of installed Windows When the installation is finished an icon Lab Power is created on the desk of Windows The Lab Power software is installed by default in the CASECOMAM directory 5 3 SPECTROPHOTOMETERS CONNECTION gt lt Specific command for Uvi Light Uvi Light X S XI XID P C pee ANUS ou or TEN UsbU vill 02 03 90307 17 j tr USB 018142 TEE se Lab Power software for Uvi Light amp UVIKON 5 3 1 UVI LIGHT XS Connect on the main the power cable of Uvi Light XS Connect the spectrophotometer to the computer through the RS232 cable Switch on the Uvi Light XS Let run the autotest Enter in the Configuration sub menu Select RS232 with left and right arrows of the keyboard Presskey ENT Uvi Light XS spectrophotometer is ready to communicate with the computer Run Lab Power software It automatically detects the spectrophotometer 9 3 2 UVI LIGHT PC Connect on the main the power cable of Uvi Light PC Connect the spectrophotometer to the computer through the RS232 cable The Uvi Light PC spectrophotometer is ready to communicate with the computer Run Lab Power software Switch on the
64. he date of factory shipment certified by air waybill consignment note or bill of lading The SCHOTT INSTRUMENTS company guarantee applies exclusively to defectiveness arising from a design fault or from a concealed defect It is strictly limited to the free dispatching of replacement parts except for consumable items or to the repairing of the equipment in our workshops within a deadline of 10 working days shipping delay not included By express agreement the following are strictly excluded from our guarantee All damages notably for staff costs loss of earnings business trouble etc Any breakdown due to an incorrect use of the equipment non adapted mains fall attempt at transformation etc or to a lack of maintenance by the user or to poor storage conditions Any breakdown due to the use of parts not supplied by SCHOTT INSTRUMENTS on SCHOTT INSTRUMENTS equipment Any breakdown due to the transporting of the equipment in packaging which is not its original packaging The lamps the cells and generally any item which appears in the accessories section on the price list Our customers are kindly asked to apply for our consent before returning any instrument for repair No return of materials may be accepted without the prior written consent of our Sales Management which will precise the terms of such return If the above consent is given articles shall be returned in their original packaging on a prepaid basis to
65. he icon Lo in the tools bar Top right of the screen Switch off the spectrophotometer Click on File and Exit or press ALT F4 5 4 4 Baseline gt lt Specific command for UVIKON XL XS Before all other operations it is advised to make a base line Click on the icon d or enter in Execute and Baseline The following window appears 21 Lab Power software for Uvi Light amp UVIKON Baseline Range s lecti X Lower limit 180 833 nm 150 Upper limit 181 300 nm 900 Data interval rnm fi Scan speed nm min 200 Close Cancel Select the wavelength range and the scan speed Click on Close It displays LabPower Junior Do not put anything inside the cell holder The unit will do a baseline correction on the air Click on OK Running baseline The spectrophotometer then executes the recording of the baseline with the selected parameters 22 Lab Power software for Uvi Light amp UVIKON rern Common functions 23 Lab Power software for Uvi Light amp UVIKON 6 GENERAL FUNCTIONS 6 1 DISPLAY SCREEN The screen display is identical in all the system methods and all the user s methods The screen is presented as follows J LabPower Junior SAMARIUM File Methods Parameters Execute View Utilities Report Window Help C as 3 5 ala Wavelength Scan ml igl Ele alal E AZ Go ajaj m SAMARIUM
66. i Light amp UVIKON 11 AUTO RATE ASSAY 11 1 CHOICE OF THE METHODE nior Methods Parameters Execute View Utilities Report Window Click on Methods New method and Autorate Assay 11 2 CHOICE OF ACQUISITION PARAMETERS Click on Parameters et Acquisition The following dialog box appears Parameters Auto Rate Assay Acquisition Wavelength 190 0 1700 0 nm Delay time 0 0 3000 0 s Averaging tine 0 1 60 0 s Ma of Readings 2 101 Reading interval 0 1 15 0 min Ho of Samples 1 6 Wavelength Choice of the measuring wavelength Delay time It is the time between the moment where the operator started measurement and the moment where the real measurement will be carried out Average time Defines the time in second used by the unit to gather the data and to calculate an average value on a reading During f f Lab Power software for Uvi Light amp UVIKON measurements with strong absorbance to reduce the noise it is recommended to use a high value No of Readings It is the number of reading between the beginning and the end of measurement Reading interval It is time between two consecutive readings on the same sample Number of samples Fix the number of samples to be measured per series If a cell changer is used the cuvettes are measured automatically 11 3 FILE OPENING Click on File et New It displays File Metho
67. ick on Save Click on NON to save the measured values Samples absorbance J LabPower Junior FIXED1 x File Methods Parameters Execute View Utilities Report Window Help Dole Pe a EASY Fed Waveren miel in Bisa E aded mie Calculated values Lin Reg No Wavel Yalue 1 500 00 77 83 2 500 00 37 16 Give a name to the file i e CALIBO2 fix and click on Save 13 1 2 9 Report setting up J LabPower Junior CALIBO2 fix Fichier M thodes Param tres Actions Voir Utilitaires Rapport Fen tre l Help O se TTE a 22 41 az Ge a x fre a E Valeurs mesur es Absorbance No LO Valeur E 1 500 00 0 8464 1 2 500 00 0 4217 1 Enregistrer saus aton O Edition Mesures calcul es R gression lin aire No LO Yaleur 1 500 00 77 832 2 500 00 37 164 Select on Report and New gt 107 Lab Power software for Uvi Light amp UVIKON F LabPower Junior CALIBO1 File Methods Parameters Execute View Utilities Report Window Help EE Fixed Wavelen oe miz Beier E AZ Go n z fin ziii M CALIBO1 2 Report Date Thurs Edit gt y Ax B CALIBOT LIBO1 1 Calculations ml x 1 Eg CALIBO2 Calculations Analysis Report i Calculated values Lin Reg Measured values Absorbance HIE No Wavel Value No Wavel Value S Operator 1 580 00 1 4482 1 1 580 00 1 4503 2 580 00 1 4482 1 2 580 00 1 4503 He
68. ile Methods Parameters Execute View Utilities Report Window Help 181 x Olesa Fixed Wavelength Je ejej 22 60 ale CALIBOT Calculated values Lin Reg No Wavel Measure S Calculation 1 580 00 1 4482 1 1 4460 2 580 00 1 4482_1 1 4460 the results These data can be inserted on the same report 13 1 2 10 Report backup 108 Lab Power software for Uvi Light amp UVIKON LabPoerer Jursor CALIBO1 2 Report i L Fle Mehrere Execute en Ulibe Report Window Meis lall Dell Fixed Wavelength miel fos 51814 El nire mimi PARAMETERS WAVELENGTH nm 560 0 DELAY 3 0 0 AVERAGING TIME ls saa 10 HO OF SAMPLES _ de 1 Reference data CALIBOT Units On LAMP CHANGE nm 338 BANDWIDTH nem 18 AUTOPRINT On File mode FIXED WAVELENGTH Sample Ho of Measurements Mode Calculation Errors Dale Time 1 z AU Lin Reg 25 02 2000 10 03 Measured values Absorbance Ho Wael value S 1 580 00 1 4482 1 2 580 00 14482 1 Calculated values Lin Reg Ho Wawel Measure 5 Cakulabon 1 380 00 1 4402 1 1 4460 2 580 00 14482 1 1 4460 Bi A Fle Methods Parameters Frere Wew ibea Repot Window sale Fined Wavelength Me aleja sed nisl rz ea E yo f Bl AL inrequtrersous ons Erreger dams E Work eme m Date Time z 202 208 TUOS Measured values Absorbance Ho Wavel Value 5 1 500 00 14402 1 AA nn 1449 1 Give a name to the file i e
69. in A position of the cell changer It is read against the air and the samples in position from B to are read against the reference er Junk ior File Methods Parameters Execute View Utilities Report Window ala x Help When the cell changer is selected Parameters Cell Changer and On selected the measurement of the samples will be done by passage of each one of them in front of the measurement beam When the cell changer is not selected Parameters Cell Changer 113 Lab Power software for Uvi Light amp UVIKON and On no selected the cell changer sets the A position of the cuvette holder in front of the measurement beam and does not move anymore The spectrophotometer behaves then as if it had one a single cell holder 14 1 3 Manual use Whether the cell changer is selected or not it is possible to move it manually to put in the measurement beam one of the positions from A to I of the cuvette holder Select View Accessories and Cell Changer Manual Control or click on the icon En The following dialog box appears Manual Cell Changer EX A E E El FIHREEERE AR It is then enough to click on the position corresponding to the cuvette to measure 14 1 4 Cell changer use inside the various measuring modes 14 1 4 1 Fixed wavelength Reference in A position Samples from B position to position 8 samples by batch The measureme
70. ion Parameters Wavelength Program X Calculation v Off C Base Point C Functions Wavelength Functions Functian OF WIL Fairs Ma of Calculations 3 WL Factors omen Wavelength Function Allows doing three types of calculation Difference Abs1 Abs2 Ratio Abs1 Abs2 Concentration Abs1 x Factor 1 Abs2 x Factor 2 on pairs of wavelengths WL1 WL2 WL2 WL3 14 Wavelengths maximum No of Calculations Enter the number of wavelengths pairs to execute a type of calculation 5 pairs maximum WL Pairs Allows defining the wavelengths pairs Example 3 WL pairs for a difference calculation WL1 WL2 WL1 WL3 WL2 WL3 WL Factors Allows defining a multiplicative factor for each WL and each pair during a concentration calculation 12 4 1 Difference measurement Example 3 wavelengths WL1 405 nm WL2 360 nm WL3 340 nm Calculation WL1 WL2 and WL1 WL3 Enter 3 in No of wavelength Select Parameters Acquisition and Set Enter the wavelength as follows 85 Lab Power software for Uvi Light amp UVIKON Wavelength value selection Wavelength Wavelength 1 nm 405 Wavelength 2 nm 360 Wavelength alnm 340 v pfi C Base Point C Functions Wavelength Functions Function WL Pairs Mo of Calculations WL Factors Select Difference for Function Enter 2 for No of Calculation
71. is Specific command for Uvi Light A part if necessary comprising particular instructions to the spectrophotometers UVIKON XS The name of this part is Specific command for UVIKON XS A part if necessary comprising particular instructions to the spectrophotometers UVIKON XL The name of this part is Specific command for UVIKON XL A part if necessary comprising particular instructions to the spectrophotometers UVIKON XS and XL The name of this part is Specific command for UVIKON XL XS 13 Lab Power software for Uvi Light amp UVIKON PARTI Star tup 15 Lab Power software for Uvi Light amp UVIKON A INSTRUCTIONS OF USE 5 1 MINIMUM CONFIGURATION RECOMMANDED Compatible PC computer Free memory on the hard disk 20 Mo minimum RAM memory 256 Mo Minimum speed of the processor 500 MHz 1 serial port RS 232C DB9 or USB port Monitor resolution min 800 x 600 pixels The Lab Power software is compatible with the following Windows versions Windows 2000 Windows NT and Windows XP Windows XP is recommended 5 2 SOFTWARE INSTALLATION FROM THE CD ROM WARNING At the time of the installation of the software the operator will select a working language French or English Once the software is installed in this language the operator will not be able any more to change it A new installation of the software will be necessary to change the working langua
72. l measurements calculations etc are finished open a new report by selecting Report and New A window presenting a new document opens Fle Methods Parameters Beake View Liites Report Window i Hei Deere Wavelength Scan Bic m aja az mja a 7 care on lel Analysis Report Date Wednesday July 19 2006 COperalor Heading WAVELENGTH SCAN Argus Pasamelerr Dirplag amp Print Specbophotonester infomation ScanRlangelnmi 155 580 Pu Mede Malle Wiseleneh ren Abs Dieta lmerval neal 100 Gea 170 Graph Range Autorange Scan Spesdinev imn 200 Ha of samples 1 Gisph Fanga E 54 f Woman 3 O AO Y ll ederi Wire Clr sei vp atm Eire 2 LB CO E al GOA 35 A text can have entered a particular place by clicking on this place with the cursor Thanks to the options below the measured values the computation results the parameters can be inserted Click on Report Insert and Values Insertion of the values measured in the form of table in wavelength scan mode this option is recommended only if the file contains only some lines Click on Report Insert and Parameters Insertion of parameters in the report Click on Report Insert and Results Insertion of the results in the form of table IMPORTANT A report can contain only alphanumeric information if one wishes to add graphs to it it is necessary to use an ex
73. ler Verweisquelle konnte nicht gefunden werden The calculation selected in Calculation Parameters will be executed on curves by selecting Execute and Calculate by pressing F3 key or by clicking on the icon ix 79 Lab Power software for Uvi Light amp UVIKON 11 5 METHODE BACKUP EA ARATEI Measure Click on Methods and Save method or press F4 key Enregistrer sous Give a name to the method i e Testcine02 pat Click on Save 11 6 MEASUREMENT gt lt Measure made with a single cell holder See chapter 14 to use a cell changer Example Measure of the activity at 500 nm during 1 mn Specific command for Uvi Light Insert the blank solution in the single cell holder Click on Execute and Autozero or click on the icon AZ Insert the sample Click on Execute and Measure or click on the icon M Measure samples one by one Specific command for UVIKON XL XS Insert blank solution and sample in the respective place in the single cell holder 80 Lab Power software for Uvi Light amp UVIKON Click on Execute and Measure or click on the icon M Insert samples one by one It displays J LabPower Junior RLACTOOL el xj Hep aerian E Ig RLACTOO 1 Calculations Calculated values sample N uiviby ll Dew
74. lick on Execute and Measure or click on the icon M Insert samples one by one Specific command for UVIKON XL XS Insert blank solution and sample in the respective place in the single cell holder Click on Execute and Measure or click on the icon M Insert samples one by one It displays Fie Methods Parameters Execute View Ulea Report Window Help ae lala sos a a Measured values Absorbance i Wael Value 5 1 500 00 0 0464_1 El 500 00 0 421 1 Measure the samples 13 1 2 7 Calculation of the samples concentration Select Execute and Calculate or click on the icon J F e Methods Parameters Execute Yew Uite Report Widow 5 m z m 5 EXE 3 El Az Go ajaj y fx D EF otiia Calculaticns i aloj x 5 37 164 It displays the concentration values of the measured samples 13 1 2 8 Results backup Select File Save as 106 Lab Power software for Uvi Light amp UVIKON PE RA Window pr ee mle Sala E zc min jene 3 a yaac MOTO Calculated values Lin Reg No Wavel Value 1 500 00 77 832 500 00 0 4217 1 500 00 37 164 ERBETEN Bieler sa leo mu y bx B CALIDO H j xc ic i Calcidalions Calculated values Lin Hey No Wawel Value 1 SELENI E z 500 00 37 164 Give a name to the file i e CALIBO1 fix and cl
75. nce C Peak Detection er Curve Operations f Activity Blank C Functions Units C Derivative C Smoothing Calculation Range Close Cancel Load Allows using a reference data file It is always a file of calibration which was created by using the program Calibration These data of calibration automatically define the factor the units and the value of the blank Factor The activity is calculated by multiplying the slope of the linear regression line in delta of absorbance per minute by the factor F Blank The blank value is always subtracted from the sample reaction It can be enter manually or defined by the data of reference file Units The selected activity units appear with the activity results when they are printed 1 katal kat 1 mol s 1 U 1 umol min 16 7 nkat 16 7 nmol s Calculation Range Click on Set It is possible to exclude from calculation part of the gathered data These points are not to erase but simply hidden 10 4 4 Functions See the same function in chapter WAVELENGTH SCAN paragraph 9 4 3 10 4 5 Derivative See the same function in chapter WAVELENGTH SCAN paragraph 9 4 4 10 4 6 Smoothing See the same function in chapter WAVELENGTH SCAN paragraph Fehler Verweisquelle konnte nicht gefunden werden The calculation selected in Calculation Parameters will be executed on curves by selecting Execute and Calculate by
76. ne iia 69 10 2 CHOICE OF ACQUISITION PARAMETERS 2 ua H ernst deine 69 10 3 FEE OPENIN Sinaloa 70 10 4 CHOICE OF CALCULATION PARAMETERS u a ai 70 POA e Peak A as need 71 TOA AE EA ONG ee sn de een ee een 71 M A Ioni T rr 71 M MNT UI AN ii 72 OP De NI CIIV GUY C Eee Seesen 72 104 0 GSMVOOUMINS T tases aOR eR L2 10 5 METHOD BACKUP HR S 73 10 6 MEASUREMENT P bh nee ea ee 73 Lab Power software for Uvi Light amp UVIKON 10 7 RESULTS BACKUP ea en une 74 10 8 EURVESOBERATIONS As ist 75 11 AUTO RATEASSAY oasis scored secveseccs E Ver ERES NRI I EID DN Gd E T 11 1 CHOICE ORETHEMETHODE 22 23 28 ro omic 71 TELA CHOICE OF ACQUISITION PARAMETERS ule trot Eo rea I 11 3 IOS dre cc 78 11 4 CHOICE OF CALCULATION PARAMETERS eere 78 IE PEE UNIT UU UTER 79 NC PE LIU m EE OOE TN OE E N 79 BAs OD OSAT A o 5 ET EE AOT TOR 79 INT MEER us AAA AAA E TOA 79 TE ADs MOON aaa aa 79 11 5 METHODE BACKUP ee A A A O o On R 80 11 6 MEASUREMENT DA A Rei 80 11 7 RESUETS BACKUP tt ceo 81 11 8 CURVES OPERATIONS 2 RA AAA A E R 82 12 WAVELENGTH PROGRAM eoan een 83 12 4 CHOICE OFTHE METHOD ads rennen 83 12 2 CHOICE OF ACQUISITION PARAMETERS oret Lon eos oe re 83 12 3 ATED COPA INN Go en ee ao seen 84 12 4 CHOICE OF CALCULATION PARAMETERS creer H nenne nnn nnne 84 IZA Dijevence measur
77. nt starts by the first sample When the time programmed in Averaging time is finished the cell holder moves on the second sample Only one measurement is done and displayed per sample The cell changer can measure also standards in Fixed Wavelength in the Calibration mode lt is necessary in this case to enter the number of standards in Parameters Acquisition and No of samples 14 1 4 2 Wavelength scan Reference in A position Samples from B position to position 8 samples by batch The full spectrum of the first sample is saved then the cell holder moved until the second sample This process continuous until the number of samples programmed in No of samples was measured 14 1 4 3 Time drive The spectrophotometer performs first the zero against the reference A position then it moves the cell holder on the B position Then it records the absorbance development of the first sample during the time programmed in Measure Time Then it comes back on the A position to perform the zero again lt goes on the second sample C position to record the development of its absorbance This process continuous until the number of samples programmed in No of samples was measured 14 1 4 4 Auto rate assay In this method the instrument can measure 8 samples simultaneously The spectrophotometer performs first the zero against the reference A position then it moves the cell holder on the B p
78. ogram EEES E Az Go ajaj a xr DUM E Kal incentrati ax Measured values Absorbance Calculated values WLFunction Concentration No Wavel Yalue_S Cycle 1 Cycle 1 1 405 00 1 6320_1 Sample 1 Sample 1 2 360 00 1 5730 1 C 1 2 C 1 3 3 340 00 0 1130_1 3 0803 3 8784 89 Lab Power software for Uvi Light amp UVIKON 12 4 4 Base point use ters Waves x r Calculation C of Reference Load Base Point C Functions J m Wavelength Functions Function Difference WL Pairs Ma of Calculati 2 oy o of Laicuiations WL Factors Has du licher NEN Fichiers de type Wavelength Program Data Ipg Annuler Close Cancel Allows cutting off from the read values data put beforehand in memory in a file and this before carrying out programmed calculation WARNING The stored data must relate on the same wavelengths number and on the same wavelengths 12 4 5 Functions use 1 Calculation Parameters Wavelength Program X Calculation C o Function add Base Point Function constant 1 Functians Wavelength Functions Function ar WL Pairs Mo of Calculations 2 WL Factors cos Allows adding or subtracting a constant from the values of absorbance before programmed calculation is carried out Allows multiplying or dividing the values of absorbance by a constant before programmed calculation is carried o
79. one on only one channel instead of two Thermo Pack NOTE The stirring system is not available 135
80. ork with a cell changer See the use of this accessory at the end of this manual By choosing Parameters and Stirrer the user can work with a magnetic stirrer and select the rotation speed of the magnet See the use of this accessory at the end of this manual By choosing Parameters and Sipper the user can work with an aspiration system and select the aspiration parameters See the use of this accessory at the end of this manual By choosing Parameters and Sample Changer the user can work with an external sample changer 30 Lab Power software for Uvi Light amp UVIKON The operator can permanently display the temperature of a sample thanks to the use a miniaturized temperature sensor See the use of this accessory at the end of this manual By choosing View Accessories and Thermosystem the operator can use the accessory named THERMOSYSTEM This accessory is composed with a Cell Changer which has its 12 positions 6 6 thermostated by Peltier effect and includes a magnetic stirrer with an external control box 6 6 STARTING AMEASUREMENT To click on the icon M or to do Execute and Measure allows running the measurement of one to ten samples whatever the selected method The acquisition parameters the common parameters and the accessory parameters are used for the measurement The measurement can cancel at every time by clicking on Ctri Alt or on the i
81. osition It makes the first measurement on the sample 1 Then it comes back on the A position to perform the zero again It moves the cell holder on the C position It makes the first measurement on the sample 2 114 Lab Power software for Uvi Light amp UVIKON This operation is repeated until the first measurement is made on the number of samples programmed in No of samples was measured Then the same process proceeds to take second measurement point on all the samples and this as many times as programmed in No of Reading 14 1 4 5 Wavelength program The reference A position and the number of samples programmed in No of samples are measured at the first wavelength Then the second wavelength is selected The reference and the samples are measured again This process is repeated until all samples were measured with all the wavelengths 14 2 SPECIFIC COMMAND FOR UVIKON XL XS 14 2 1 Installation Switch off the spectrophotometer and connect the accessory cable on the connector located on the accessory board inside the cuvette well Switch on the spectrophotometer And run Lab Power software The cell changer is automatically recognized during the initialization 14 2 2 Activation The cell changer is setting in 6 samples 1 reference mode during the initialization 14 2 3 Operating mode of cell changer The cell changer has 3 operating modes It is possible to select these operating modes
82. pressing F3 key or by clicking on the icon i 72 Lab Power software for Uvi Light amp UVIKON 10 5 METHOD BACKUP Oj Load User Method New Method A Pa Save Method Click on Methods and Save method or press F4 key Enregistrer sous Give a name to the method i e TDRIV ptm Click on Save 10 6 MEASUREMENT gt lt Measure made with a single cell holder See chapter 14 to use a cell changer Example Measure of the activity at 500 nm during 1 mn Specific command for Uvi Light Insert the blank solution in the single cell holder Click on Execute and Autozero or click on the icon AZ Insert the sample Click on Execute and Measure or click on the icon M Measure samples one by one Specific command for UVIKON XL XS Insert blank solution and sample in the respective place in the single cell holder 73 Lab Power software for Uvi Light amp UVIKON Click on Execute and Measure or click on the icon M Insert samples one by one It displays Pie Methods Parsmetes Dote Wew Ubljes Repot Window Ojea Timenme miej ofS aja il ule SLOVWELACI Calbculations Caloulaled values Actlreity Sample M fMctivity Std Dev CD Calculation range O 20 min sample 1 0 0636 0 0045 OFFS p Acquitition Parameters Display amp Print Warvelergth nent 5800 Peri Mode Values
83. r raw absorbance values Conc01 fix for concentration values 49 Lab Power software for Uvi Light amp UVIKON Q WAVELENGTH SCAN 9 1 CHOICE OF THE METHOD File Methods Parameters Execute View Utilities Report Window Help D Load User Method INC de sis afm sis E 6 fal Click on Methods New method and Wavelength Scan 9 2 CHOICE OF ACQUISITION PARAMETERS Click on Parameters et Acquisition The following dialog box appears Parameters Wavelength Scan Acquisitian Lambda min 130 1099 nm Lambda mas 131 1100 nm Data interval rnm Scan speed nmmn Ma of cycles 1 10 Cycle time 0 0 900 0 min Ho of samples 1 6 Cancel Lambda min Minimum wavelength of the wavelength range Lambda max Maximum wavelength of the wavelength range B1 Lab Power software for Uvi Light amp UVIKON Data Interval It is the wavelength gap between two points of measurement This gap is fixed for the spectrophotometers Uvi Light and UVIKON XS and adjustable for UVIKON XL Scan speed It is the scrolling speed of the wavelength for the measurement The proposed speeds nm min are 100 200 400 600 850 1250 1800 2400 No of cycles It is the number of times that the measurement of spectrum will be repeated on the same sample maximum 10 Cycle time It is the time of a cycle expressed in minutes This time include
84. ration data To come back to the values table select View and Table or click on 13 1 1 9 Backup of the calibration curve To save the calibration curve select File and Save as Y LabPower Junior SWATCH pcf File Methods Parameters Execute View Utilities Report Window Dlel rlalelS carixeawaveiengn MD ja 2 4 zlc ms a fuar EE Bi CALIBO1 pcf Calibration data CALIBO1 pcf Data FWSTD con y Amde LL Save the calibration curve i e CALIBO1 con 100 Lab Power software for Uvi Light amp UVIKON 13 1 1 10 Deleting of a value If a point were badly measured it is possible to erase it and to measure it again This is available for several points For this operation proceed in the following way a bus p 4 zu se 5306 Select File and Edit Standards Edit Calibration File Nominal Measured Ma 0 O M tn e o M LE l C3 Delete all measures Delete Line ca Select the standard to be measure again and click on Delete line i e Standard 3 101 Lab Power software for Uvi Light amp UVIKON Edit Calibration File Nominal Measured Sample Ma E E LI mo ca co cm Rm to Pa SEE Delete all measures Delete Line ca Re register the concentration of the standard which one has just removed under the concentration of the last standar
85. s time necessary to measurement Number of samples Fix the number of samples to be measured per series lf a cell changer is used the cuvettes are measured automatically 9 3 FILE OPENING Click on File and New It displays LabPower Ju LAD mor WS Methods Parameters Graph Mare A default file will be open and its name will be WScan1 Measure 9 4 CHOICE OF CALCULATION PARAMETERS Click on Parameters and Calculation 52 Lab Power software for Uvi Light amp UVIKON CalculationParameters Wavelength Scan Calculation Values v Off C Peak Detection C Curve Operations C Functions Derivative C Smoothing caen Several types of calculation can be applied to the values of absorbance Peak Detection Curve Operations Functions Derivative omoothing 9 4 1 Peak detection CalculationParameters Wavelength 5can X Values Peak Mode pks yall Feak Sensitivity 0 1 Absorbance Calculation Off Peak Detection C Curve Operations C Functions C Derivative C Transmittance C Smoothing caen Peak Mode Choose peaks and valleys detection in the same time or only peak or valleys detection Peak Sensitivity Choose the sensitivity threshold for the detection Value between 0 0001 and 999 9 Absorbance Transmission Indicate on which type of curve the detection will be made curved in absorbance or curve in transmi
86. spectrophotometer when the computer requests it The software detects automatically the Uvi Light PC and runs the autotest 9 3 3 UVI LIGHT XT AND XTDimension Connect on the main the power cable of Uvi Light XT or XTD Remove cable between the spectrophotometer base and internal computer inside the cover Connect the spectrophotometer base to the computer through the RS232 cable The Uvi Light XT XTD spectrophotometer is ready to communicate with the computer Run Lab Power software A dialog box requests to switch the unit on Switch on the Uvi Light XT XTD Press immediately OK in the software dialog box There are around 5 seconds before the Uvi Light closes the communication port The spectrophotometer is automatically detected and then starts its autotest 18 Lab Power software for Uvi Light amp UVIKON Specific command for UVIKON XL XS UVIKON XL XS IE lt a eno ous or E LisbUvikond2 02 93 90307 5 3 4 UVIKON XL XS Connect on the main the power cable of UVIKON Connect the spectrophotometer to the computer through the RS232 cable Switch on the spectrophotometer System failure Measure Halogen lamp Deuterium lamp Power on Wait until the Power on light is flashing Run Lab Power software It detects automatically the UVIKON and starts the autotest 19 Lab Power software for Uvi Light amp UVIKON 5 4 INITIALIZATION
87. ssion Select the type of curve in agreement with the displayed curve 53 Lab Power software for Uvi Light amp UVIKON 9 4 2 Curve operations CalculationParameters Wavelength Scan Calculation Values Reference C Off Peak Detection Selected sample No Curve Operations C Functions Function C Derivative C Smoothing caen Allow to add to subtract to multiply or to divide all the curves displayed in the main window by another curve selected by the operator Select the curve which will be used for this operation by clicking on Load Select the type of operation Multiplication division subtraction or addition 9 4 3 Functions CalculationParameters Wavelength Scan Calculation Values Function C Off Peak Detection Function constant Curve Operations Functions C Derivative C Smoothing coc Allow to add or to subtract a constant value from the absorbance values positive or negative shift of the curve Allow to multiply or to divide the absorbance values by a constant value increasing or decreasing of the curve Allow to calculate the logarithm of the absorbance values Thus allow to increase peaks with low amplitude Enter the function Enter the constant value Click on Close 54 Lab Power software for Uvi Light amp UVIKON 9 4 4 Derivative CalculationParameters Wavelength Scan E X Values
88. ter Display amp Print Scam Rangel 121 580 Pin ode Values Dala interval bm T0 Gee 1700 Sean Speed min 200 Mo amper 1 Graph Ranga w Aui Graph anges m0 mii The Zoom function can be repeated by selecting a new zone until obtaining the maximum resolution Select View and Full View or press on the Esc key to cancel the zoom effect NOTE It is also possible to cancel the zoom effect while clicking on the icon El or by clicking on the mouse right button 9 8 5 Absorbance Transmittance function ower Junior SAMARIUM Calculations alt Fie Methods Parameters Execute View Utiles Report Window TE Djelsiiel a ale waw e m c ma aji 12 60 ale a xt fcoc on rue Y Absorbance This function allows having the curve displayed in absorbance or in transmittance Displayed in absorbance mode Select View and Absorbance Displayed in transmittance mode Select View and Transmittance 9 8 6 Graphics Table function This function makes it possible to display either the spectral curve or the table of the values which were used to make this curve 61 Lab Power software for Uvi Light amp UVIKON Displaying of the curve Select View and Graphics or click on the icon Displaying of the values table Select View and Table or click on the icon Example
89. ternal text processing able to import text and files BMP exported with the clipboard To avoid any loss of information for a question of page setting click on File and Print Preview 37 Lab Power software for Uvi Light amp UVIKON 6 17 PRINTING OF THE RESULTS Click on File and Print or Ctrl P to print data or results The following dialog box will be open Imprimante Norn DeskJet BA0C 642C 648C Copie 1 Fraopri t s tat Type HP DeskJet 400642076460 Emplacement LPT1 Commentaire Imprimer dans un fichier Copies Nombre de copies one d impression Tout Pages def al gu Copies assembl es Annuler f S lection 38 Lab Power software for Uvi Light amp UVIKON PART III Measuring method 39 Lab Power software for Uvi Light amp UVIKON T FLOW SHART Whatever is the method of measure ations are always held in the same order Refer to the flow chart below Choice of the measurement method Choice of the measurement parameters New file opening Choice of calculation parameters Method backup Sample measurement Possible calculation on the results Backup and printout of the results 41 Lab Power software for Uvi Light amp UVIKON o FIXED WAVELENGTH METHOD 8 1 CHOICE OF THE METHODE F Labfower i homor Fie Methods Prometey Emo e Wer Uses Report Window L
90. to ten curves depending on the selected number of samples can be displayed on the screen during measurement 14 2 4 2 Fixed wavelength Measurement starts with the first sample After the selected Averaging Time the cell changer will move to the second sample Only one reading per sample is measured and displayed The cell changer can also be used to measure standards in the Calibration mode 14 2 4 3 Wavelength scan The complete scan is carried out on the first sample before the cell changer moves to the second position and measurement of sample 2 is carried out This process continues until all selected samples have been measured 14 2 4 4 Time drive The complete time drive is carried out on the first sample before the cell changer moves to the second position and measurement of sample 2 is carried out This process continues until all selected samples have been measured 14 2 4 5 Wavelength program All samples are measured at the first wavelength then the wavelength is changed to the second one and all samples are measured again This process will be repeated until all wavelengths on all samples have been measured 120 Lab Power software for Uvi Light amp UVIKON 15 SIPPER gt lt 15 1 SPECIFIC COMMAND FOR UVI LIGHT 15 1 1 Sipper selection Connect the peristaltic pump to the spectrophotometer and select Auto on the box Run Lab Power software Y LabPower Junior Des
91. ture can be program by writing the value or qui 10 0 zi by selecting it with arrow keys This value is taking in account by clicking on Set et Gradient programming It is programmable in the same way as temperature It is activated or not by clicking on the button close the display 10 0 I Temperature gradient is activated 10 0 5 eue Temperature gradient is not activated The stirring rate is selected using Min Max buttons Stirring Min LLL Manx 132 Lab Power software for Uvi Light amp UVIKON 18 1 5 Alarms COMMUNICATION ERROR Thermo System Sample e Reference C 2 Check communication between the 6 6 cell changer and the control box OUT OF LIMIT TEMPERATURE Thermo System Sample E Reference C 2 The temperature is displayed in red as long as it has not reached its programmed value TEMPERATURE DETECTION ERROR Thermo System Sample JE Reference C e This message is displayed if there is no communication with the temperature probe if this one is out of order 133 Lab Power software for Uvi Light amp UVIKON 1 9 e IHERMOPACK The THERMOPACK is a thermostatisation system of an internal liquid by PELTIER which allows the complete compatibility and connection with all thermostatable accessories of UVIKON and Uvi Light spectrophotometers The THERMOPACK has the same functionalities as the THERMOSYSTEM except the temperature control which is d
92. um contained in the origin file Each new file represents a spectrum converted in UV PRO format Example The file Test scn containing two spectra will become after export in UV PRO format a whole of two new files with the names of Test UV Pro 01 scn Test UV Pro 02 scn NOTE It is possible to export several curves in only one operation one only needs after having opened the repertory in which the spectra are to select them by group with the key Shift or individually with the key Ctrl Then enter for each one of them information with regard to them NOTE If the spectrum acquisition were made with a too small measurement step it is possible that the number of measurement points for a given wavelength range is higher than 850 points In this case the software displays the following error message LabPower There is to many points in this spectrum for the exportation with UYPRO formal The U PAG spectrum file iz limited to 850 points Do you want to apply the automatic reduction algorithm OK Cancel The user can either to use the algorithm proposed by the software in order to recompute the number of points or to make a new acquisition of the spectrum by reducing the number of measurement point or the wavelength range or both 6 11 4 Bitmap bmp format The data files in graphic form can be transformed into bitmap files for Windows BMP for their use in te
93. uring the impression of the curve Select View and Heading It displays Printout Heading X Heading SAMARIUM test Cancel Enter the heading and click on OK During the curve printing the heading will appear on the left top of the page See below rin LS re LANZ 9 8 10 Display Samples function Allows selecting when there are several curves on the screen those which will remain displayed Select View and Display Samples It displays Display samples X fe Sample MW Sample amp M Sample2 MW Sample 7 Cancel Iw Samples IM Sample 8 M Sampled W Samples I Sample 10 IY Sample 5 All samples 66 Lab Power software for Uvi Light amp UVIKON Select the samples will appear on the screen 9 8 11 _ Baseline range function This function makes it possible the operator to know on which wavelength range the last baseline was made Select View and Baseline range It displays View baseline range X ast baseline correction executed between 400 and 500 nm 130 nm S00 nm a Fermer The operator knows that the last baseline was made between 400 nm and 500 nm 67 Lab Power software for Uvi Light amp UVIKON 1 0 TIME DRIVE 10 1 CHOICE OF THE METHODE o File Methods Parameters Execute View Utilities Report Window DE c ers a azc mim A IE Click on
94. ut Calculate the logarithm of the measured values of absorbance before calculation is carried out Enter the function Enter the constant Click on Close 90 Lab Power software for Uvi Light amp UVIKON 12 5 METHOD BACKUP fal Incentrati A Click on Methods and Save method or press F4 key Enregistrer sous Give a name to the method i e Difference plp Click on Save 12 6 MEASUREMENT gt lt Example Difference measurement between 2 absorbances read at two different wavelengths ADS405nm ADS3eonm Specific command for Uvi Light Click on Execute and Measure or click on the icon M It displays 91 Lab Power software for Uvi Light amp UVIKON J LabPower Junior OMECYCLE Ple Method Parameters Execute View Unites Report Window Help ala sel me fal Difference aa OMECYCLE 1 Hieasare Measured values Absorbance Calculated values WiLFunction Difference Ko Warvel Value amp Cycle 1 A Inner your cumple and click on OK pogon Paara ir Display amp Pri Hoof wavelength PiniMnde Va Delap i 80 Cycles Neat 1700 A amp weagng me t 10 Mee tenpler Insert the reference and click on OK Then insert the sample and click on OK Measure samples one by one Specific command for UVIKON XL XS Insert blank solution and
95. xt processing softwares of image processing software Choose File Export and Bitmap Choose the directory give a name to the file and make Save 6 11 5 Towards clipboard The data in the form of graphs or of numerical values can be exported towards the clipboard of Windows to be immediately imported in another application by one copy paste Choose File Export and Clipboard 34 Lab Power software for Uvi Light amp UVIKON 6 12 USER S METHODS 6 12 1 Backup If a document is open in an unspecified method and that the status bar indicates Ready all the parameters Acquisition parameters common parameters and accessory parameters can be saved Select Methods and Save M The following dialog box appears DELL MEE x Enregistrer dans C3 WORK em er ER 5A FIXED pfx Mom du fichier Type Longueur d onde fixe pfx Annuler E Select the directory Give a name to the method Click on Save 6 12 2 Downloading A set of parameters Acquisition Calculation Basic can be re used if they are previously saved in a user method To download a user method USER MTH Click on Methods et Load User Method or click on the icon Select the method and click on Open The main tools bar displays the name of the user method in the display area located close to the icon
96. y light All the values can be selected between the limits Under operation the setting in position of the filters is automatic and transparent for the user Specific command for UVIKON XL The dialog box which displays the parameters appears 28 Lab Power software for Uvi Light amp UVIKON Basic parameters Optics Automation Printout Optics Lamp Change 290 400 nm Bandwidth nm Response Time s Filter Change 00 850 nm Fiter Change 540 580 nm Filler Change 470 490 nm Filter Change 340 380 nm Fiter Change 260 300 nm Cancel Lamp Change 290 400 nm It is the wavelength of commutation between deuterium lamp and halogen lamp All values between 290 and 400 nm are available by default 338 nm Bandwidth Spectrophotometer bandwidth expressed in nanometers It is by default selected at 2 nm and can be change by the user The following values are available 0 2 0 5 1 0 2 0 4 0 nm Response Time It is the time between two data displaying on the screen Filter Change Each one of these parameters preset a wavelengths range in which the user can change the point of commutation of the filters which cut the stray light All the values can be selected between the limits Under operation the setting in position of the filters is automatic and transparent for the user 6 4 2 Automation index Basic parameters Optics Automation Printout Actions Automatic

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