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User Manual - Spectra Services

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1. Data Lock LampReady Home Shutter Lamp Alarm Bit 5 Bit 4 Bit 3 Bit 2 Bit 1 Bit 0 Decimal On Off Yes No Fault Pass Open Close On Off On Off 0 X X X X X X 1 X X X X X X 2 X X X X X X 3 X X X X X X 4 X X X X X X 5 X X X X X X 6 X X X X X X 7 X X X X X X 8 X X X X X X 9 X X X X X X 10 X X X X X X 11 X X X X X X 12 X X X X X X 13 X X X X X X 14 X X X X X X 15 X X X X X X 18 X X X X X X 19 X X X X X X 22 X X X X X X 23 x X X X X X 26 x X X X X X 27 X X X X X X 30 X X X X X X 31 X X X X X X 32 X X X X X X 33 X X X X X X 34 X X X X X X 35 X X X X X X 36 X X X X X X 37 X X X X X X 38 X X X X X X 39 X X X X X X 40 X X X X X X 41 X X X X X X 42 X X X X X X 43 X X X X X X 44 X X X X X X 45 X X X X X X 46 X X X X X X 47 X X X X X X 50 X X X X X X 51 X X X X X X 54 X X X X X X 55 X X X X X X 58 X X X X X X 59 X X X X X X 62 X X X X X X 63 X X X X X X 29 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 13 Graphical User Interface for X Cite 120 PC Control Panel 13 1 1 The purpose of the GUI Graphical User Interface is to provide control of the X Cite 120 PC functions via a remote PC 13 2 X Cite 120 PC Software Minimum PC Configuration Requirements 13 2 1 The following are the minimum requirements for a PC to be used wi
2. 19 Warranty Lumen Dynamics warrants the original purchaser for a period of one 1 full year calculated from the date of purchase that the equipment sold is free from defects in material and workmanship In the event of a claim under this guarantee the equipment is to be sent postage and carriage paid including a description of the fault to the Lumen Dynamics Service Center Returned equipment will not be received without a Return Authorization RA Number issued by the appropriate Service Center In the case of damage caused by wear and tear careless handling neglect by the use of force or in the case of interventions and repairs not carried out by a Lumen Dynamics Service Center the guarantee ceases to be valid This guarantee may not form the basis for any claims for damages in particular not for compensation of consequential damages The warranty is not transferable No warranty is extended to perishable items such fuses air filters and light guides Any claims for units received with defects in material or 51 LUMEN DYNAMICS e e X Cite Fluorescence Illumination In Control workmanship must be reported to an authorized Lumen Dynamics Service Center within 30 days from the original date of receipt Replacement Bulb Warrant If the X Cite SERIES 120Q bulb fails to strike during the warranty period of 2000 hours the bulb will be replaced under warranty or a credit will be applied to the purchaser s account In
3. 30 13 3 Installing X Cite 120 PC Control Panel Software rrrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 30 ii LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 13 4 X Cite 120 PC Connection sise 31 13 5 Controlling Unit Functions via the Graphical User Interface 31 14 Messages IndicatorS rnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 34 15 Clearing an Audible Alarm deeg geet 36 at E BE 36 161 aerer einen te arte eee sieste entire een 36 16 2 Liquid ELE Lua nn en nt 38 e GET GS EE ae nie 41 16 4 Air V nis amp FIG S anses eege A akea a EAEE EEA tt amande 42 17 Routine Care and Maintenance ns 44 17 1 General tips to help maximize lamp lifetime 45 18 Technical Specifications meer venmenredaddeknnmnnndnee 46 18 1 Lamp MOIS che cnerertimakeneicrdimesreteiideneseecaeieuneeaeieeme eae 46 18 2 Light OURS A EEE 47 18 3 e ee E 48 18 4 Lamp Power electrical ss 48 18 5 Environmental Operating Conditions rrrnnnnnnnnnnnnnnrrrnnnnnnnnrernnnrrrnnnenenerennnnnnn 48 18 6 Transport and Storage Conditions 49 18 7 Regulatory Compliance ss 49 18 8 China en LEE 51 19 Warranty E 51 20 Eoptaetlpotorrsthont eessen See 53 201 Web SOP eege 53 20 2 Service Centers acces sn en et cousin eee ete 53 20 3 Replacement Pants da rinetsneretenendaiinemmeennsnten nmentitre 53 iii LUMEN DYNAMICS X Cite Fluorescence
4. section of the light guide becomes noticeably warmer than the rest of the guide a bubble is blocking transmission of light forcing the light guide to absorb the energy Itis 2 3 years old OR has been in use for 4000 6000 hours 2 3 lamp changes 40 LUMEN DYNAMICS e e X Cite Fluorescence Illumination In Control 16 3 Lamps Performance and lifetime 1 The lamp output spectra and typical output levels over lifetime are shown below Relative Output X Cite 1200 vs HBO 100 100 Relative Intensity arbitrary units amp 600 800 1000 1200 1400 1600 1800 2000 Elapsed Time Hours 2 Itis normal for the output to decline over the first several hundred hours of use and then stabilize at a level 70 of the initial output for the remainder of the lamp life If the lower power regions of the output spectra are being used it may be desirable to replace lamps more frequently to maximize signal levels 41 LUMEN DYNAMICS PUTTING YOU IN CONTROL x Cite Fluorescence Illumination In Control 16 4 Air Vents amp Filters X Cite units have several air vents that are an integral part of the cooling system and overall lamp performance Proper cooling ensures that lamps operate at optimal temperature and pressure for output power spectrum lamp life light guide life and safety Ensuring adequate cooling 1 Never obstruct the air vents on the X Cite unit Vents are loc
5. LUMEN DYNAMICS x Cite Fluorescence Illumination In Control If the LED display indicates SFI 1 This indicates there is a shutter failure 2 Power down the unit wait 1 minute and turn the power back on 3 If the unit once again displays the shutter failure message contact your local X Cite Service Center If the LED display does not light 1 If the fan is functional power down the unit wait approximately 20 seconds then power it up again 2 If the problem persists contact your local X Cite Service Center If either Fan does not work properly 1 If the LED display is functional power down the unit wait approximately 20 seconds then power it up again 2 If the problem persists contact your local X Cite Service Center 16 2 Liquid Light Guides Proper handling and installation of liquid light guides 1 Liquid light guides have a typical useful life of 4000 hours of operation when handled properly and installed in a well maintained X Cite 120 The formation of bubbles is one of the most common reasons for a light guide to degrade prematurely and result in a sudden reduction in illumination intensity Bubbles can form without warning and if they occur within the first 1500 to 2000 hours of use is it typically due to overheating and or mechanical stress to the light guide Below are some simple tips to avoid overheating and stressing the light guide 2 Always fully insert the light guide in to the X Cite unit
6. 1 3 All commands sent to the X Cite 120 PC must be a carriage return terminated Cd in C code or chr 13 in basic code The following commands listed are based on C code When the carriage return character CR is received and there is no error in the command the X Cite 120 PC will acknowledge with a carriage return r If there is an error the X Cite 120 PC will acknowledge with eV 24 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 12 4 Communication Protocol MAC Version MAC Interface OS Compatability e MAC OS 10 e MACOS 9 USB adapter type 12 4 1 e Keyspan P N USA 19H e Adapter information can be found at http Awww keyspan com products usb USA19HS Command timing and execution duration areas per the RS 232 specified requirements See section 12 5 12 5 Command Timing Specification 12 5 1 12 5 2 12 5 3 12 5 4 12 5 5 There are four levels of timing to which the end user must adhere in order to ensure reliable performance of the system Port Timeout Pre Write Delay Post Write Delay and Post Read Delay Port Timeout refers to the minimum amount of time required for data to be received by the PC Failure to adhere to this requirement will result in dropped responses as well as other undesirable effects if further attempts to communicate with the unit are made The Pre write delay begins immediately after the execution of one command Once this delay h
7. A A A A A X Cite Fluorescence Illumination In Control Warning Disconnecting of main supply source is only possible by unplugging the power cord Warning This unit is designed for bench top use only Always ensure that the unit is operated on a hard stable surface This will prevent obstruction of the bottom chassis ventilation openings Any obstruction of these openings could result in a possible over heating condition Do not attempt to remove or tamper with the rubber feet located on the bottom of the unit Danger This unit contains HIGH VOLTAGE components It is recommended that ONLY QUALIFIED TECHNICAL PERSONNEL perform any testing or repairs described in this manual Disconnect the AC power cord from the unit before opening the cover of this unit All cover screws must be replaced prior to applying power to the unit or safety of the unit will be impaired Monitoring the unit during manual operation The level of UV energy supplied by the X Cite 120 PC is sufficient to ignite flammable substances During manual operation the unit must be attended at all times by a qualified operator The unit must not be left unattended while turned on If an operator leaves the work area of the unit the lamp power switch must be turned off Monitoring the unit during Automated operation The level of UV energy supplied by the X Cite 120 PC is sufficient to ignite flammable substances Therefore when the unit is operated unattended in
8. REN 4 3 Safety Information 5 6 sition 5 3 1 Glossary of SN seende 5 EE 5 4 Installing the Lamp Module nes 8 5 Inserting and Removing the Light Guide rrnnnnnnnvvvvnnnnnnnnnnnnnnvvnnnnnnnnnnnnnnnnvennnenr 12 5 2 Tips to prevent premature degradation of light guides rrrrrrnnnnnonnnnnnnrrnnnnn 14 6 installing the Collimating Adapter ss 15 7 X Cite 120 PC Message Reference nes 17 8 Powering Up and Powering DOWN anssvvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnsnnnnnnnevennnenr 18 9 Adjusting the Light Output Intensity ss 19 10 Timed OSU OS ee 20 10 2 Adjusting the Exposure NG iso cnet weenie henna ee 20 10 3 Running a Timed Exposure ss 20 11 Additional Functions 5 ete es 21 11 1 Viewing the Accumulated Lamp Hours NENNEN 21 11 2 Locking Unlocking the Up Down Adjustment Buttons eect 21 11 3 Using the Foot Pedal Remote Device 22 12 seele ee eege EE 22 121 K EN ne 22 12 2 Serial RS 232 Interface is 22 12 3 Communication Protocol PC Version iceiscssiecsteianetacustecaienctiamseseneeestuceeaine 23 12 4 Communication Protocol MAC Version 25 12 5 Command Timing Specifications 25 125 Command Set TIMING Lesser SSES 26 12 7 Graphical User Int lat6 Luunsarmmmmnsnnkmmmevvo vre SES 26 ER ET ga 00 EE RE eeaee een eee Serer eee eee 27 123 Unit Status TE iesen 29 13 Graphical User Interface for X Cite 120 PC Control Panel 30 13 2 X Cite 120 PC Software Minimum PC Configuration Requirements
9. i e up to the white line this ensures contact with a heat sink to conduct heat away from the light guide 38 LUMEN DYNAMICS x lt Cite Fluorescence Illumination In Control 3 Always allow adequate clearance at the rear of the X Cite unit and microscope to prevent excessive bending and or crushing of the light guide against walls Minimum bend radius to prevent immediate damage to the LLG is 40mm however a bend radius of at least 75mm is recommended for a light guide while in use Sharper bends can cause heat to build up and cause problems longer term Observei 4 Always place the X Cite unit close enough to the microscope so that there is some slack in the light guide and no sharp bends 5 Never leave an endcap on the output end of the light guide when the other end is connected to the X Cite unit if the unit is turned on in this condition the cap will overheat melt and or permanently discolour the quartz end of the light guide 6 Do not expose the light guide to extreme temperatures above 35 C below 5 C for extended periods of time during use transport or storage this may cause degradation of the seals and allow air bubbles to form in the liquid What does a bubble look like Depending on the size and location in the light guide a bubble may or may not be obvious To check for bubbles 1 Disconnect the LLG from the X Cite and microscope adapter 2 Hold one end towards a bright windo
10. Mode and the shutter is closed TE ER ER NER EA i I ee a ae ea oe AS E 1 Note When the unit is connected to a PC or the front panel has been manually locked exposure settings can not be adjusted from the 4 X Cite 120 PC front panel buttons 10 2 Adjusting the Exposure Time 10 2 1 Set your X Cite 120 PC to Timer Mode by pressing the Mode button until the four digits are displayed as XXX X The number shown will indicate the exposure time in seconds and tenths of a second 10 2 2 Press the up button to increase the exposure time by 1 10 sec or press the down button to decrease the exposure time by 1 10 sec 10 2 3 For coarse adjustments press and hold the up or down button to rapidly increase or decrease the exposure time 10 3 Running a Timed Exposure 10 3 1 Ensure that your X Cite 120 PC unit is set to timer mode or lamp hour mode as per section 11 1 10 3 2 Press the START STOP button The shutter will automatically open and the exposure time will begin to count down When the exposure time has elapsed to zero the shutter will automatically close and the timer mode will automatically reset to the preset value 10 3 3 If the X Cite 120 PC is in timer mode and a timed exposure is activated the display will show the remaining time while the shutter is open 10 3 4 To stop a timed exposure during an exposure cycle press the START STOP button The shutter will immediately close and the exposure time will automatically
11. Never obstruct the air vents on the X Cite unit Vents are located at the rear and underside of the unit Note In earlier X Cite models vents were located on the side underneath the lamp access panel c Do not remove the rubber feet on the X Cite unit or otherwise reduce block the space between the bottom of the unit and bench top This may compromise airflow through the unit d Ensure that the air being used to ventilate the X Cite unit is approximately room temperature e g do not place the X Cite unit on top of another heat producing instrument e Ifa heated environmental chamber is being used for live cell imaging make sure that the X Cite unit and the light guide are located outside of the chamber Do not expose the light guide to extreme temperatures above 35 C below 5 C for extended periods of time during use transport or storage this may cause degradation of the seals and allow air bubbles to form in the liquid Never kink bend crush or stretch the light guide this type of mechanical stress may cause bubbles to form in the liquid and or damage to the outer sheath a Always allow adequate clearance at the rear of the X Cite unit to prevent excessive bending b Place the X Cite unit close enough to the microscope so that there is some slack in the light guide and no sharp bends 14 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 5 2 6 Never leave an endcap on the output end of
12. START STOP button to clear the audible alarm Power down the unit remove the lamp access cover and replace securely tightening the fastening screw in place Wait a few minutes and turn power on to the unit If it still does not strike replace the lamp The LED display indicates the cool message This indicates the lamp is too hot to strike The lamp will automatically strike when it has cooled The LED display indicates alternately end then bulb This indicates that the lamp has accumulated over 4000 hours and will not strike The lamp module must be replaced If the light intensity is too low check that 1 2 The light guide has been fully inserted into the light guide retainer The X Cite 120 PC unit includes an iris adjustment Change the system to Intensity Mode and adjust the of iris opening using the UP button to increase the illumination See section 9 Adjusting the Light Output Intensity There are no foreign substances on the emitting end of the light guide There are no bends kinks or other physical damage to the light guide Replace the light guide if there is any physical damage The LED display indicates alternately old then bulb This indicates that the lamp has accumulated over 2000 hours and may require replacement It may be necessary to replace the lamp or the light guide Contact your sales representative for information on purchasing a new lamp or light guide 37
13. an automated environment an alarm function must be provided by the user to indicate a malfunction in the associated equipment used Warning Hg LAMP CONTAINS MERCURY Manage in Accord with Disposal Laws see www lamprecycle org or 1 800 668 8752 Danger Exposure to Mercury represents a health hazard to humans When unpacking or installing the lamp always wear protective clothing and a face mask Operate lamp only in the X Cite 120 lamp housing This prevents direct viewing of the arc and in the case of lamp bursting contains the lamp particles In the rare instance in which a lamp bursting occurs and the mercury content is released the following safety precautions are recommended all personnel should be immediately evacuated from the area to prevent inhalation of the mercury vapour The area should be well ventilated for a minimum of 30 minutes Prior to clean up ensure an approved mercury respirator mask and non porous gloves such as latex or rubber are used After the lamp housing elements have cooled the mercury residue should be collected with the use of a special absorbing agent available from laboratory equipment suppliers 6 LUMEN DYNAMICS PEEP SG GE X Cite Fluorescence Illumination In Control Listed below are examples of internet web sites for obtaining Mercury Spill Kits e htto www rosshealthcare org Mercon spill kits htm e htto www coleparmer ca catalog product _index asp cls 43577 e http www en
14. for bench top use only Always ensure that the unit is operated on a hard stable surface This will prevent obstruction of the bottom chassis ventilation openings Any obstruction of these openings could result in a possible over heating condition Do not attempt to remove or tamper with the rubber feet located on the bottom of the unit 5 Inserting and Removing the Light Guide 5 1 1 Make sure that power is off to the X Cite 120 PC unit 5 1 2 Ensure that the protective end caps are removed from both the input and output ends of the light guide prior to installation 5 1 3 Insert the light guide into the light guide retainer located on the back panel of the unit Push the light guide in until it seats with a second positive click Reference Figure 9 Figure 9 Light Guide Insertion 12 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control Tip When the light guide is fully inserted the line on the light guide insertion label will be right up against the light guide port Note If the light guide is not inserted properly it may result in reduced light output and overheating which can cause premature degradation ofthe light guide 5 1 4 During installation or removal grasp the light guide on the strain relief nearest the input end of the light guide Reference Figure 9 Note Never grip the light guide during installation or removal by any other part other than the strain
15. inner surface or the outer surface of the reflector If touched carefully clean the envelope with alcohol Skin oils can etch the glass and cause premature bulb failure c Wear cotton gloves or powder free latex gloves when handling any lamp Ensure that air filters are kept clear of dust and debris even a partially blocked filter can reduce lamp lifetime to approximately 25 of what is normally expected 45 LUMEN DYNAMICS x lt Cite Fluorescence Illumination In Control 18 Technical Specifications 18 1 Lamp module A D Ceramic Components B Reflector C Bulb E Intelli Lamp Connector F Lamp Power Connector Lamp Module High Pressure 120 watt mercury vapor short arc Lamp Module Life 2000 hours typical Warm Up 90 seconds typical 46 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 18 2 Light Guide Light Delivery Flexible liquid filled light guide 1 5m or 3m in length with a core diameter of 3mm Custom light guides are also available Liquid Light Guide End Fitting ai gm i 0 0 d P d 7 PS vE d A CROIS IN 1 Liquid Light Guide Dimensions in mm Core End Fitting End Fitting Minimum Bend Radius mm do d1 d2 3 5 9 40 47 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 18 3 Power Input Power Supply Input Voltage Current Input Surge Protection Fuse Rating High efficiency switch mode constant
16. ranging from manufacturing to bio medicine and we are unmatched in our commitment to quality and service The heart of the X Cite 120 is a proprietary 120 watt short arc lamp with an improved 2000 hour typical lifetime This extended lifetime is made possible by the proprietary thermal management system incorporated into the design The lamp is mounted in an elliptical reflector with a proprietary coating to provide unequalled spectral and power output The new X Cite Series 120PC has also been improved for much quieter operation Standard features incorporated in the X Cite 120PC include the pre aligned Intelli Lampe system an accumulative lamp hour meter and a lamp status indicator The X Cite 120PC is a high quality product manufactured in accordance with ISO 9001 CE marked and certified to UL and CSA standards We suggest that you read this manual to discover all features and how to use them 1 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 2 Checking the contents of the box Carefully unpack the unit and accessories Store the packing material for future use 2 1 Box Contents 1 X Cite 120 PC Illumination Un 2 Lamp Module X Cite 1 20 vaca ecaiic sca cestennesedecedesaseascdsGasesdeasnaxeoneseveeteesatenseacee Collimating Adapter yas esse dunes nanas rentes eme irene druknet 4 Light Guide X Cite 120 8mm x 1 5m or 3mm x 3 0m 5 Power Cord IEC gro
17. the event of a claim under this guarantee the lamp is to be sent postage and carriage paid including a description of the fault to the Lumen Dynamics Service Center Returned equipment will not be received without a Return Authorization RA Number issued by the appropriate Service Center Lamps must be purchased from an authorized Lumen Dynamics Representative or Distributor to be eligible for the warranty replacement This warranty is non transferable In the case of damage caused by careless handling neglect by the use of force or in the case of interventions and repairs not carried out by a Lumen Dynamics Service Center to the X Cite 120 system the guarantee ceases to be valid Returning equipment to Lumen Dynamics 1 Please make a note of the problem encountered the steps followed to isolate the problem and the result of any trouble shooting steps taken 2 Contact the nearest Lumen Dynamics Service Centre to obtain a Return Authorization Number For your convenience RA numbers can also be requested on line at http www ldgi xcite com support need serviced php 3 Follow shipping instructions provided by the service technician The unit should be returned in its original packaging if possible Please do not ship the unit with the lamp installed 52 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 20 Contact Information Lumen Dynamics Tel 905 821 2600 Fax 905 821 2055 1 800 668 8752 US
18. the light guide when the other end is connected to the X Cite unit if the unit is turned on in this condition the cap will overheat melt and or permanently discolour the quartz end of the light guide 5 2 7 While the X Cite unit is on but not in actual use i e during sample preparation or between time lapse time points close the iris shutter on the X Cite this reduces unnecessary UV photon load on the liquid light guide Using only the shutter stop in the microscope itself protects the specimen but energy is still passing through the light guide 6 Installing the Collimating Adapter 6 1 1 Ensure that the power is off on the X Cite 120 PC unit 6 1 2 The existing lamp housing must be removed from the microscope before the collimating adapter can be mounted Do not discard the existing mounting hardware since it will be used to secure the collimating adapter to the microscope 6 1 3 Remove the collimating adapter from its packaging Note The collimating adapter has been set at the factory No adjustments by the customer are required 6 1 4 Insert the flange portion of the collimating adapter into the lamp port of the microscope Using the existing hardware tighten the screw s until the flange is fully secured 6 1 5 Remove the protective cap from the output end of the light guide Insert the light guide into the input portion of the collimating adapter until it is flush with the adjustable insert Secure the light guide
19. to the collimating adapter by tightening the thumbscrew Do not over tighten Note The light guide has a minimum bend radius of 1 6 inches 40 0mm Bending or coiling the light guide tighter than this radius will result in permanent damage to the light guide 15 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control ET TE ee E DE ae ke AE E Warning Turning power on to the X Cite 120 PC unit without the light guide properly installed in the unit and in the microscope can increase your risk of exposure to UV light 16 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 7 X Cite 120 PC Message Reference Symbol Message Description Displays the accumulated hours the lamp has XXXX Lamp Hours been on XXX X Exposure Time Displays the time in seconds the shutter will remain open when activated XXX Iris Setting Displays the iris setting in a percentage of maximum Flashin pona g Flashing display The lamp is warming up Warm up time is XXX X approximately 90 sec Lamp installed incorrectly bulb Bulb Error Lamp did not strike or extinguished after striking Lamp is too hot to strike cool Cool Warning The lamp will automatically strike when it has reached optimum striking temperature old bulb Alternating Old and then Bulb The lamp has accumulated over 2500 hours Lamp may be near end of life end bulb Alternati
20. used to allow data to be transferred to and from a PC to perform a variety of functions all of which are initiated by a program that executes on the PC 22 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 12 3 Communication Protocol PC Version 12 3 1 This section provides the detail description of the commands between the X Cite 120 PC unit and PC Command Description Connect Verifies communication between the PC and the X Cite 120 PC Returns an error if communication is not established Lock Front Panel Locks the X Cite 120 PC front panel buttons A LOC message is flashed on the display for a few seconds The display defaults to lamp hour mode Unlock Front Panel Unlocks the X Cite 120 PC front panel buttons Get Software Version Returns the software version from the X Cite 120 PC Get Lamp Hours Returns the accumulated lamp hours from the X Cite 120 PC Get Unit Status Returns the current X Cite 120 PC status panel lock unlock alarm conditions shutter position lamp on off amp shutter faults Refer to Appendix 1 for details Clear Alarm Clears the alarm beeper if the alarm is active Run a timed exposure Opens the X Cite 120 PC shutter iris to the set intensity for the set exposure time The intensity and time must be set by the user see commands below The system will use the previously saved settings when this command is a
21. A and Canada x cite ldgi com www dgi xcite com 20 1 Web Store www ldgi xcitestore com 20 2 Service Centers For a complete list of authorized service centers please visit www Idqi xcite com asc php 20 3 Replacement Parts Replacement lamps and light guides can be purchased directly from Lumen Dynamics For ordering and pricing information contact the inside sales department at x cite ldgi com www ldgi xcite com 1 800 668 8752 Part Number Description 012 63000 X Cite 120 lamp module Collimating Adapter call to match microscope 805 00038 X Cite 120 Light guide 8mm x 1 5m 805 00040 X Cite 120 Light guide 8mm x 3 0m Grounded power cord IEC call to match AC voltage 850 00023R Hex Key 3mm Lamp access cover 031 00027R X Cite 120PC Q CD Users guide 035 00329R X Cite 120PC Q English Quick Start 53 LUMEN DYNAMICS
22. AL IIe Fluorescence Illumination e In Control SERIES 120PC Q USER S GUIDE Printed in Canada 035 00328R Rev 1 X Cite Fluorescence Illumination In Control X Cite 120 PC User s Guide Lumen Dynamics Group Inc 2011 All rights reserved No part of this publication may be reproduced transmitted transcribed stored ina retrieval system or translated into any language in any form by any means without the prior written consent of Lumen Dynamics Group Inc Every effort has been made to ensure information in this manual is accurate however information in this manual is subject to change without notice and does not represent a commitment on the part of the authors X Cite 120PC Control Panel Software Minimum Computer Specifications 300 MHz processor Pentium or equivalent Windows 98 2000 or XP 32 Mb RAM 20 Mb for Software Installation 20 Mb for Data Storage SVGA video 800 x 600 resolution One available RS 232 Port Trademarks X Cite 120 PC and Intelli Lamp are registered trademarks of Lumen Dynamics Group Inc All other product names are trademarks of their respective owners LUMEN DYNAMICS X Cite Fluorescence Illumination In Control Table of Contents 1 Jee Helene 1 2 Checking the contents of the boOXx ssssvvrnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnevnnnnnr 2 21 BOX COMENS username en stile eeuantab weed ydewnngecteb esoneny EREA PEREKI R 2 22 FOR diel nende 3
23. Illumination In Control Table of Figures Figure 1 Front Panel WEEN se 3 Figure 2 Rear Panel ViGW siceccicceccccisncnsccrettecneieetiesctenenenencraneesenrceeisadientnaeenenerteeesen eaters 4 Figure 3 Lamp AGGSSS E 8 Figure 4 Lamp Housing gasellene 9 Figure 5 Lamp Orientation E 9 Figure 6 Intelli Lamp Connection wisicessesetcnsieceicedendasounvietesatesausauniabesanesd casasecebeiseesuxenuvede 10 Figure 7 Lamp Power Connection ss 11 Figure 8 Lamp Wire Orientation nnne ennet 11 Figure 9 Light Guide EE 12 Figure 10 Collimating Adaptor nn nes souclatacdemertasaveluiatedebienigesbetendeenetadweniane 16 iv LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 1 Introduction The X Cite Series 120PC represents a new standard in fluorescence illumination It gives you the convenience and control never before available in a fluorescence illuminator and has been designed to let you focus your energy where it belongs on obtaining the best possible results The X Cite 120PC joins the Lumen Dynamics family of light systems offering the same high level of innovation quality and reliability that our commercial customers have come to expect Since 1982 Lumen Dynamics has combined next generation optical engineering state of the art electronics and fibre optics to produce sophisticated technologies that employ light Today Lumen Dynamics is a leading developer of light based systems for sectors
24. Up Down push buttons 1 Push and hold the Mode button 2 While pressing the Mode button press the following sequence of buttons DOWN UP UP Up 3 After 2 seconds the message ULOC will momentarily appear for 2 seconds then vanish 21 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 11 2 4 Once the system is unlocked all of the front panel buttons will perform as per their normal function 11 3 Using the Foot Pedal Remote Device 11 3 1 To use a foot pedal or other remote control device to control the shutter on your X Cite 120 PC system insert a 3 5mm audio style plug into the jack located on the rear panel 11 3 2 Depressing the foot pedal or providing a momentary contact closure will provide the same function as pressing the START STOP button The shutter will open as per the current mode setting of the unit 11 3 3 The foot pedal connector a 3 5mm audio style jack has the following pin out connections Connection Point Signal Name Outer rim Common GND Center pin Positive input active low 12 RS 232 Specifications 12 1 Introduction 12 1 1 The X Cite 120 PC software is designed to provide remote control of the microscope illumination system from a PC 12 1 2 The X Cite 120 PC software requirement specification detailed below describes the communication protocol between the X Cite 120 PC unit and the PC 12 2 Serial RS 232 Interface 12 2 1 The RS 232 serial interface is
25. as passed the next command may be sent Failure to adhere to this delay will result in dropped commands The Post Write delay is required primarily for the Set Intensity Level command Once this command is sent the Post Write delay begins and the user cannot poll for system response until the delay has expired Failure to adhere to this requirement will result in dropped responses as well as other undesirable effects if further attempts to communicate with the unit are made The Post Read delay begins immediately after unit response from a particular command is received Once the delay has expired the user may continue executing commands Failure to adhere to this requirement will result in dropped commands and responses 25 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 12 6 Command Set Timing Command Port Timeout Delay Type Delay Connect 30ms Pre Write 50ms Lock Front Panel 30ms Post Read 1000ms Unlock Front Panel 30ms Pre Write 50ms Get Software Version 30ms Pre Write 50ms Get Lamp Hours 30ms Pre Write 50ms Get Unit Status 30ms Pre Write 50ms Clear Alarm 30ms Pre Write 50ms Run a timed exposure 30ms Post Read 250ms Open Shutter 30ms Post Read 250ms Close Shutter 30ms Post Read 250ms Get Intensity Level 30ms Pre Write 50ms Set Intensity Level 30ms Post Write 50ms Shutter Closed Set Intensity Level 30ms Post Write 600ms Shutter Opened Ge
26. ated at the rear and underside of the unit Note Arrows indicate direction location of air flow In earlier X Cite models vents were located on the side underneath the lamp access panel pe 2 Always leave clearance for air flow between the X Cite and walls or other equipment Do not remove the rubber feet on the X Cite unit or otherwise reduce block the space between the bottom of the unit and bench top This may compromise airflow through the unit 42 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 3 Ensure that the air being used to ventilate the X Cite unit is approximately room temperature e g do not place the X Cite unit on top of another heat producing instrument If a heated environmental chamber is being used for live cell imaging make sure that the X Cite unit and the light guide are located outside of the chamber Periodically check the air filter at the air intake vent for debris Replace or clean as necessary How to check clean X Cite 120 PC metal vents 1 When unit is OFF and lamp is COOL unplug power from X Cite unit and turn unit upside down Visually inspect the metal gratings for lint dust and other debris Debris can be removed from the gratings using a small vacuum cleaner with a crevice tool or blown out with a can of compressed air If using compressed air direct the air flow ACROSS the vents not perpendicularly i e into the X Cite unit What hap
27. cal Equipment for Measurement Control and Laboratory Use Part 1 General Requirements UL Std No 61010A 1 Electrical Equipment for Laboratory Use Part 1 General Requirements Electromagnetic Compatibility EN 61326 1997 A1 1198 A2 2001 IEC 61326 2002 Electrical Equipment for Measurement Control and Laboratory Use Electromagnetic Compatibility FCC Class A Digital Device or Peripheral Information to User NOTE This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Warning Changes or modifications not expressly approved by Lumen Dynamics could void the user s authority to operate the equipment 49 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control WEEE Directive 2002 96 EC The symbol above indicates that this product should not be disposed of along with municipal waste that the product should b
28. cess panel and replace securely tightening the fastening screw in place Wait a few minutes and turn power on to the unit If it still does not strike replace the lamp 5 old bulb The old and then bulb message appear in alternating sequence for about 10 seconds when power is first turned on to the unit if the accumulated lamp hours have exceeded 2500 hours When this message occurs it is recommended that the lamp be replaced soon 34 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 6 end bulb The end and then bulb message appear in alternating sequence when power is first turned on to the unit if the accumulated lamp hours have exceeded 4000 hours This indicates that the lamp has reached the end of safe life If the lamp hours reach 4000 hours safety measures will prevent the lamp from striking The lamp must be replaced at this point 7 cool The cool message advises the user that the lamp must cool down before re ignition It appears on the display when power is turned on to the unit and the lamp is too hot to strike Striking the lamp when it is too hot will reduce lamp life The lamp will automatically strike once it has reached optimum temperature 8 LOC The LOG message appears for about 2 seconds when the UP DOWN adjustments are initially locked It also appears if the up or down button is pressed while they are locked advising the user that these functions are
29. ctivated Open Shutter Opens the X Cite 120 PC shutter iris to the set intensity until a close command is received Close Shutter Closes the X Cite 120 PC shutter Get Intensity Level Returns the current X Cite 120 PC iris intensity setting 0 12 25 50 or 100 Set Intensity Level Shutter Closed Sets the X Cite 120 PC iris to the specified intensity 0 12 25 50 or 100 The shutter remains closed The setting will be saved until the next Set Intensity Level command is received Set Intensity Level Shutter Opened Sets the X Cite 120 PC iris to the specified intensity 0 12 25 50 or 100 The shutter remains open The setting will be saved until the next Set Intensity Level command is received Get Exposure Time Returns the current X Cite 120 exposure time 0 2s to 999 9s Set Exposure Time Sets the X Cite 120 PC exposure time to the specified value 0 2s to 999 9s The setting will be saved until the next Set Exposure Time command is received Turn Lamp ON Switches the lamp on if no alarms are detected Lamp must have been previously turned off Turn Lamp OFF Switches the lamp off The system power remains on 23 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 12 3 2 Com Port Configuration Baud rate 9600 Data bits 8 Parity None Stop bits 1 Flow Control None 12 3 3 Message Format and Protocol
30. e collected separately and that a separate collection system exists for all products that contain this symbol within member states of the European Union e The equipment that you bought has required the extraction and use of natural resources for its production It may contain hazardous substances that could impact health and the environment e In order to avoid the dissemination of those substances in our environment and to diminish the pressure on the natural resources we encourage you to use the appropriate take back systems Those systems will reuse or recycle most of the materials of your end life equipment in a Sound way e The crossed out wheeled bin symbol indicated above invites you to use those systems e If you need more information on the collection reuse and recycling systems please contact your local or regional waste administration 50 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 18 8 China RoHS The following table contains substance information for the X Cite 120PC Q as required by China RoHS regulations AeA PAP EE EE BBE A FR IET RHR 120W PRAAN ERRA ITER A ED al ERE X 1 ET o FETT BUTE RIPE o KIRA ETTE ETE SHS SJ T11363 2006 R ELEN IR BERD F X RTE RE EVNE VEGEN GE Hi Ses SJIT11363 2006r ENE NER ATTER gt HR Char ET BIE AT JE A
31. e completed by qualified repair personnel only VAN If the unit fails to power up use the following checklist to eliminate the most common causes of problems Check that 1 2 3 4 The AC power cord is securely plugged into a functional AC wall plug The AC power cord is securely plugged into the AC inlet on the rear of the unit The mains AC power switch is in the ON position Check that the ventilation openings on both the bottom and rear of the unit are not blocked VAN If the unit still does not power up 1 Check both mains power fuses by first disconnecting the power cord Then carefully remove the fuse drawer assembly below the AC inlet on the rear of the unit If the fuse s is are open replace with the same type 4A 250 V Fast acting 36 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control If the LED display lights and the fan starts but the lamp won t turn on check if 1 The LED display indicates the bulb message and the system begins to beep This indicates that no lamp has been detected Check if the lamp has been installed correctly Refer to Section 4 Installing the Lamp Module The LED display indicates the bulb message after a 20 or 30 sec delay and the system begins to beep This indicates that the lamp has failed to strike It may be a result of the lamp reaching end of life or that the lamp access cover is not secured properly in place Press the
32. g UV light and high levels of visible light will be emitted from the light guide Opening the shutter on the X Cite 120 PC without the light guide properly installed in the unit and in the microscope can increase your risk of exposure to UV light Press the START STOP button to manually open the shutter The open LED indicator on the front panel will illuminate Press the up button to increase the light output or press the down button to decrease the light output The percentage of the iris opening displayed will increase or decrease to 0 12 25 50 or 100 each time the up or down button is pressed PE EE ee 1 Note No light will be emitted from the unit when the iris opening is set to 0 or if the START STOP button has not been pressed to activate the shutter L Press the start stop button to manually close the shutter The open indicator LED will turn off once the shutter has closed open the shutter The shutter will remain open until the START STOP button is pressed once again to close the shutter Use this mode to L override the timer function if manual operation of the shutter is required 19 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 10 Timed Exposures 10 1 1 Your X Cite 120 PC system includes a timer to automatically close the shutter after a user selected amount of time Adjustments to the exposure time can only be made when the X Cite 120 PC unit is in Timer
33. he lamp bracket arm Reference Figure 5 4 1 7 Locate the 4 pin Intelli Lamp sensor connector multi coloured wiring harness at the rear of the lamp module and connect it to its mate located on the lamp housing wall Reference Figure 6 Figure 6 Intelli Lamp Connection Tip The Intelli lamp connector will only attach in the correct orientation If you are having difficulty attaching the connector try rotating it by 180 10 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 4 1 8 Attach the 2 pin lamp power connector to its mate on the lamp housing wall Reference Figure 7 Figure 7 Lamp Power Connection Tip The 2 pin lamp power connector will only attach in the correct orientation If you are having difficulty attaching the connector try rotating it by 180 4 1 9 Ensure the Intelli Lamp sensor and the lamp power leads are secured into the appropriate alignment grooves of the lamp housing Reference Figure 8 WIRE SECURING Figure 8 Lamp Wire Orientation 11 LUMEN DYNAMICS Cite Fluorescence Illumination In Control 4 1 10 Replace the lamp access panel and tighten the fastening screw Reference Figure 3 Ss a ee SA Note If the lamp module has been installed incorrectly the message l bulb will appear on the display and a continuous audible beep will be heard after the 90 second warm up cycle has been 2 completed ER ER ER VAN Warning 1 This unit is designed
34. llowable range will appear Select Cancel and re enter an acceptable value EE EEE ENN ge ee EN REE 1 Note If another value is not selected the X Cite 120 PC will automatically retain the value that was previously selected prior to the error message being displayed L_ 32 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 13 5 5 Exposure Settings Disabling Exposure Timer To disable the exposure timer de select the checkbox next to Exposure timer active This will allow the shutter to remain in the open or closed position as per the shutter control without any set timing function 13 5 6 Shutter Control To activate the shutter control once the appropriate intensity and exposure settings have been entered click on the Open button in the Shutter Control section a aes a ee Fe me ee a GEN GOE eet 1 Note During a timed exposure cycle when the shutter is open a red bullet will appear next to Exposure Unit Status indicator will display Shutter Open After the exposure cycle has been completed the shutter will close and the exposure time will automatically reset The Unit Status indicator will display Shutter Close To close the shutter during an exposure cycle click on the Close button 13 5 7 Alarm Function The following conditions will cause the unit to indicate an alarm condition i Lamp fail to strike ii Lamp access door not co
35. locked out See Section 11 2 Locking and Unlocking the UP DOWN Adjustment Buttons 9 ULOC The ULOC message appears for about 2 seconds after unlocking the UP DOWN adjustment buttons to advise the user they have been unlocked See Section 11 2 Locking and Unlocking the UP DOWN Adjustment Buttons 10 SFI The SFI message appears if there is a shutter failure Power down the unit wait a minute and turn power on to the unit If the unit displays a shutter failure again contact your local X Cite Service Centre to have your unit serviced 1 LAMP The LAMP indicator is the top of two green LED s located to the left of the display The LAMP LED illuminates when the lamp is on The LAMP LED will flash when the lamp is warming up 2 OPEN The OPEN indicator is the bottom of two green LED s located to the left of the display The OPEN LED illuminates when the shutter is open 35 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 15 Clearing an Audible Alarm 15 1 1 The X Cite 120 PC provides an audible alarm to alert the user of various error conditions The following conditions will cause the unit to indicate an alarm condition i Lamp fail to strike ii Lamp access door not correctly installed iii Intelli lamp sensor connection failure iv Shutter position failure 15 1 2 To clear the audible alarm 1 Press the START STOP button 16 Troubleshooting 16 1 General Service to b
36. ng End and then Bulb The tamp NaS mer J000 AONTA The lamp will not strike The UP DOWN adjustment buttons have been LOC Adjustment Locked locked No changes can be made to the exposure time or iris setting The UP DOWN adjustment buttons have been ULOC Adjustment Unlocked unlocked Changes can be made to the exposure time and iris setting The shutter has failed Unit should be SFI Shutter Failure restarted If the error repeats unit should be serviced See Section 16 for troubleshooting LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 8 Powering Up and Powering Down Lamp Warm Up The ARC lamp has 3 distinct phases of operation 1 lgnition 2 Warm up Lumen Dynamics recommends 20 minutes of proper warm up and to ensure a stable optical output 3 Stable Operation It is recommended that phase 1 and 2 are not interrupted This can result in shortened lamp life The lamp must be allowed to warm up uninterrupted 8 1 1 Ensure that the lamp and light guide have been properly installed and that the lamp access cover is securely fastened 8 1 2 Plug the X Cite 120 PC unit into a properly grounded AC outlet 8 1 3 Set the main power switch I O located on the front panel to the I ON position and check the fan for airflow 8 1 4 The lamp will automatically turn on within 20 seconds and the lamp indicator LED on the front panel will illuminate The display will flash for
37. of the X Cite 120 PC application window 30 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 13 4 X Cite 120 PC Connection 13 4 1 Connect the rear panel RS 232 port on the X Cite 120 PC unit to an available COM port on your PC using the 9 pin cable assembly supplied 13 4 2 Ensure that your X Cite 120 PC unit is plugged in a functional AC outlet 13 4 3 Turn on the front panel power switch and wait until the 90 second warm up period has been completed front display has stopped flashing 13 4 4 Next on the GUI in the Session Function control block click your mouse on the Connection list box to select the appropriate COM port link Select COM 1 or COM 2 etc 13 4 5 Next click on the Connect button If the wrong COM Port number has been selected then the following error message will appear No response from unit If this occurs click on Cancel and re select the appropriate COM port ERT EE EE EE EEE EEE EE EE EE E VEE ee ee EE ER ME ER 1 Note Once the appropriate link has been established all the functional buttons on the graphical user interface will become enabled high lighted Disabled non functional control buttons are greyed out 13 5 Controlling Unit Functions via the Graphical User Interface 13 5 1 Once a COM link has been established between the X Cite 120 PC unit and the user s PC the front panel controls and rear panel analog jack cont
38. pens if filters are not clean 1 A complete filter blockage or airflow obstruction generally results in an automatic lamp shutoff within 10 15 minutes of the unit being powered on due to lack of adequate cooling A partially clogged filter may allow enough airflow for continued operation but not for optimal cooling In this case the result is usually dramatically reduced lamp life e g 400 hours instead of 2500 hours 43 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 17 Routine Care and Maintenance 1 Operate the unit in a well ventilated area with at least six inches clearance at the rear of the unit for proper air flow Do not place any objects below the unit or between the feet as this will restrict airflow through the bottom of the front face plate 2 For safe operation use only a grounded outlet Gei Avoid physical shocks or jarring to the unit especially while the unit is operating Such sudden movements reduce the lamp module life gt The lamp module must be operated for a minimum of 20 minutes each time it is turned on to prevent damaging the lamp Increasing the time between turning the lamp module on and off will maximize lamp life 5 Replace the air filters frequently to ensure unrestricted air flow It is recommended as a minimum that the air filter be removed and washed with a mild detergent and water every time the lamp module is replaced Note restricted airflow can ca
39. power output 100 240 V 50 60Hz 2 4 1 0A 100 240V 50A max cold start Short circuit auto recovery Over voltage up to 135 5 of nominal EMI filtering integrated into the PFC module Lamp driver circuit has integrated thermal cut off Dual fuse system each fuse rated at F4A 250V Fast acting type 18 4 Lamp Power electrical Lamp Voltage Output Power Max Lamp Voltage Lamp Drive Frequency Line Regulation 85V RMS square wave 50 duty cycle 1 4A typical Lamp nominal 120W 115V RMS 150 Hz From min Vin to max Vin lamp 2 18 5 Environmental Operating Conditions Operating Temperature Altitude Humidity Atmospheric Pressure Over voltage Category Pollution Degree 5 to 402 C max 2000m max 15 to 90 RH non condensing 70 to 106 kPa Il 2 48 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 18 6 Transport and Storage Conditions Temperature 40 to 70 degrees Celsius Relative Humidity 10 to 95 RH non condensing Atmospheric Pressure 500 to 1060 kPa 18 7 Regulatory Compliance C 4 Complies to the following directives standards Council Directive 73 23 EEC Low Voltage Directive Council Directive 89 336 EEC EMC Directive Safety Compliance EN IEC 61010 1 2001 Safety Requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements CAN CSA 1010 1 1992 Safety Requirements for Electri
40. relief portion 5 1 5 To remove the light guide firmly grip the strain relief near the light guide retainer and pull out firmly F Note The X Cite 120 PC is designed for use with a 3mm liquid light guide Lumen Dynamics can not guarantee the performance of the X Cite 120 PC if using light guides other than those supplied by Lumen Dynamics 13 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 5 2 Tips to prevent premature degradation of light guides Refer to section 16 for detailed set up and maintenance tips 5 2 1 5 2 2 5 2 3 5 2 4 5 2 5 Liquid light guides have a typical useful life of 2 3 years in the X Cite 120 PC when installed and handled properly The formation of bubbles is one of the most common reasons for a light guide to degrade prematurely and result in a sudden reduction in illumination intensity Bubbles form without warning usually due to overheating and or mechanical stress to the light guide Below are some simple tips to avoid overheating and stressing the light guide The light guide has a minimum bend radius of 1 6 inches 40 0mm Bending or coiling the light guide tighter than this radius will result in permanent damage to the light guide Make sure the light guide is cooled properly during use and prevent overheating a Always fully insert the light guide in to the X Cite unit this ensures contact with a heat sink to conduct heat away from the light guide b
41. reset The open indicator will turn off 20 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 11 Additional Functions 11 1 Viewing the Accumulated Lamp Hours 11 1 1 Your X Cite 120 PC system automatically accumulates the number of hours that the lamp is on and shows this information on the LED display The lamp hours are shown when the unit is in Lamp Hour mode 11 1 2 Set your X Cite 120 PC to Lamp Hour Mode by pressing the Mode button until the four digits are displayed as XXXX a flashing decimal point to the right This shows the number of accumulated hours on the lamp SS SSS SS SS SS SS SS SS E EE E 1 Note Pressing the START STOP button while in Lamp Hour mode will open the L shutter for the pre set Exposure Time see Section 10 11 2 Locking Unlocking the Up Down Adjustment Buttons 11 2 1 Your X Cite 120 PC system allows you to lock or disable the Up Down buttons located on the front display panel When the system lock has been engaged no adjustments to the exposure time or iris opening can be made This can help to ensure constant exposures when the X Cite 120 PC is utilized by multiple operators 11 2 2 To Lock the Up Down push buttons 1 Push and hold the Mode button 2 While pressing the Mode button press the following sequence of buttons DOWN UP UP UP 3 After 2 seconds the message LOC will momentarily appear for 2 seconds then vanish 11 2 3 To Unlock the
42. rols become disabled The display will indicate elapsed lamp hours only The Unit Status indicator on the GUI will indicate Lock On 13 5 2 Lamp On Off Control 1 The lamp in the X Cite 120 PC is turned on automatically once the front panel power switch on the unit is set to the ON position 2 Toremotely turn the lamp off click on the Turn Lamp Off button in the Lamp Control section on the GUI The following message will be displayed Are you sure you want to turn the lamp off Click on Yes to confirm The lamp will shut off and the Unit Status indicators will confirm by showing Lamp Off and Lamp Ready No To turn the lamp back on click on the Turn Lamp On button Note During the lamp warm up cycle or if the lamp is to hot to strike both Lamp On and Lamp Off buttons will be disabled until the warm up cooling cycle has been completed Once the Turn Lamp On button has been selected the lamp will turn on automatically when the unit has cooled to the appropriate temperature J 31 LUMEN DYNAMICS K Cite Fluorescence Illumination In Control 13 5 3 Exposure Settings Intensity Level Intensity level can be selected by the following 2 different methods 13 5 4 Method 1 Click on the Up Down buttons located right of Intensity Level to select the desired level 0 12 25 50 or 100 Method 2 Click on the arrow of the Intensity Le
43. rrectly installed iii Intelli lamp sensor connection failure iv Shutter position failure The alarm condition is indicated by a repeating audible beep from the X Cite 120 PC unit The Unit Status indicator on the GUI will display Alarm On To clear the alarm signal click on the Clear Alarm button The audible alarm on the unit will cease and the Unit Status indicator will display Alarm Off In addition to clearing the alarm signal on the GUI the X Cite 120 PC unit must have the front panel power switch reset to clear the alarm condition The fault which caused the original alarm condition must be corrected prior to resuming normal operation ea ee ee EE RR EN EE EE EE EE 1 Note The Unit Status indicators on the GUI may be beneficial in diagnosing any potential causes relating to the alarm condition Refer to Section 13 for additional information on troubleshooting 33 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 13 5 8 Accumulated Lamp Hours The X Cite 120 PC s accumulated lamp hours are indicated in the Misc Functions section of the GUI When the COM link for the unit has been established the GUI will automatically update the unit s accumulated lamp hours To update the accumulated lamp hours during remote operation click on the Get button 14 Messages Indicators The X Cite 120 PC display and LED indicators provide information to the user to aid in
44. rt gt FlushInBuffer ComPort gt FlushOutBuffer UnitResponse ComPort gt PutChar 0x74 II Command t Connect ComPort gt PutChar 0x74 II Command t Connect ComPort gt PutChar r II Command r TimeOut false TimeOutTimer 1 Wait for response from unit while TimeOutTimer Application gt ProcessMessages if UnitResponse e r Unit got wrong command MessageDlg Command is wrong mtError TMsgDlgButtons lt lt mbCancel NULL Screen gt Cursor crDefault return else if UnitResponse r Unit got correct command Cmd1 gt Enabled false Cmd3 gt Enabled true Cmd4 gt Enabled true Cmd5 gt Enabled true Cmd6 gt Enabled true Cmd7 gt Enabled true Cmd8 gt Enabled true Cmd9 gt Enabled true Cmd10 gt Enabled true Cmd11 gt Enabled true Cmd12 gt Enabled true Cmd13 gt Enabled true Screen gt Cursor crDefault return 27 LUMEN DYNAMICS s X Cite Fluorescence Illumination In Control if TimeOut Unit did not get command MessageDlg No response from Unit mtError TMsgDlgButtons lt lt mbCancel NULL Screen gt Cursor crDefault Application gt ProcessMessages 28 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 12 9 Unit Status Table
45. s panel using the 3mm hex key provided and remove the panel sui 3MM HEX KEY LAMP ACCESS Figure 3 Lamp Access Panel 4 1 3 Carefully remove the lamp module from its container holding only the ceramic components Caution The lamp modules operational life can be significantly shortened if handled incorrectly Be sure only to handle the ceramic surfaces Do not touch the bulb s glass envelope or the inside surface of the reflector Skin oils can cause the lamp module to fail prematurely 8 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 4 1 4 Open the lamp bracket arm by pulling towards you refer to Figure 4 LAMP POWER LAMP CONNECTOR BRACKET LAMP SPRING CLAMP LAMP BRACKET ARM INTELLI LAMP Figure 4 Lamp Housing 4 1 5 Position the lamp so that the two leading edges of the lamp ceramic mount slide into the groove of the lamp bracket The middle of the lamp should be in position to fit into the spring clamp Reference Figure 5 Tip Make sure that the This side out label is facing outwards before trying to insert the lamp as illustrated below Reference Figure 5 THIS SIDE OUT Figure 5 Lamp Orientation 9 LUMEN DYNAMICS AL Ter Fluorescence Illumination In Control 4 1 6 Slide the lamp all the way in so that both leading edges of the lamp ceramic mount are in the groove of the lamp bracket The middle of the lamp will snap into the spring clamp Close t
46. t Exposure Time 30ms Pre Write 50ms Set Exposure Time 30ms Pre Write 50ms Turn Lamp ON 30ms Pre Write 50ms Turn Lamp OFF 30ms Post Read 50ms 12 7 Graphical User Interface 12 7 1 When the X Cite 120 PC is connected to a PC the unit front panel will be automatically locked The unit status will be sampled at twice per second 12 7 2 The following screen shot is the GUI application window which will appear on the user s PC H x Cite 120 PC Control Panel E Exit rShutter Control Open Exposure Settings ity Level 5 rSession Function Ee 12 Connection COM2 rLamp Control Turn Lamp ON Turn Lamp Off M Exposure Timer Active jol x Misc Functions Software Version Close Lamp Hour H Get Up Unit Status Alarm On Off Down Shutter Home Fault Pass Set 2 Lamp On Off fir Lamp Ready s Yes 1 No Up Shutter Open Close Lock On Off Down set Clear Alarm Note For detailed GUI operating instructions refer to Section 12 7 26 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 12 8 Sample Code Example Borland C code for command Connect void fastcall TForm1 Cmd1Click TObject Sender d signed char Tries 4 Try 4 times communication before declaring no response from the unit Screen gt Cursor crHourGlass Show hourglass cursor Application gt ProcessMessages while Tries ComPo
47. t from directly viewing ultraviolet light protective eye shielding and clothing must be used at all times Input Output Signals Input Signal 3 2 Safety Precautions The X Cite 120 PC is equipped with two safety sensors to protect the user from accidental UV exposure In addition please observe the following precautions during use This series of cautions warnings and dangers relate to the operation and maintenance of the X Cite 120 PC They are also presented throughout this User s Guide where necessary Warning Eye damage may result from directly viewing the light produced by the lamp used in this product Always use protective eyewear and turn the lamp off before removing cover Caution Never look into the light emitting end of the light guide The light could severely damage the cornea and retina of the eye if the light is observed directly Eye shielding must be used at all times as well as clothing to protect exposed skin Warning Always make sure the light guide is properly inserted into the X Cite 120 PC and the microscope prior to turning on power to the unit This will minimize the risk of exposure to the UV light Caution To prevent damage degradation of the light guide always allow adequate clearance at the rear of the X Cite unit to prevent kinking or excessive bending Warning To reduce the risk of fire or shock always replace the fuses with the same type and rating gt gt E 5 LUMEN DYNAMICS
48. th the X Cite 120 PC Control Panel software 300 MHz recommended Pentium or equivalent processor 32 MB RAM 10 MB available storage for software installation 20MB additional storage suggested for your data files SVGA video 800 X 600 resolution 8 bit color 16 bit color or better recommended Available RS 232 COM port required for one time setup procedure and for remote unit operation 13 2 2 Operating System Requirements Microsoft Windows 95 98 NT 2000 ME or XP 13 3 Installing X Cite 120 PC Control Panel Software 13 3 1 Perform the following to install the X Cite 120 PC software Turn on the PC to be used with the X Cite 120 PC system Shut down any other Windows programs currently in use Insert the X Cite 120 PC Control Panel software CD in the CD ROM drive of your PC Right click your mouse on the Windows Start button and select Explore Left click on Explore and select D Double click on SETUP EXE Next follow the setup instructions as they appear by clicking next each time the user prompt appears until the installation has been completed and finish appears Click on finish to complete the installation To access the control panel software program click on the Windows Start menu and select programs X Cite 120 PC Control Panel X Cite 120 PC Control Panel A screen with a title bar displaying X Cite 120 Control Panel will appear on your monitor Refer to Section 17 5 for sample screen shot
49. the warm up period of 90 seconds The display will stop flashing when the warm up cycle has been completed Wait until the warm up cycle has been completed before using the X Cite 120 PC to perform an exposure Lumen Dynamics recommends 20 minutes to ensure a stable output Note Unit should not be turned off unless the lamp has been on for a d minimum of twenty minutes Note Ifthe lamp is turned off and an attempt is made to turn it back on before it has fully cooled the cool message will appear on the display The lamp will automatically re strike when the lamp has cooled l 8 1 5 To power down the unit set the mains power switch I O to the O OFF position 18 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 9 9 1 1 Adjusting the Light Output Intensity Your X Cite 120 PC system includes an iris adjustment to control the amount of light output from the unit Adjustments to the output can only be made when the X Cite 120 PC unit is in Intensity Mode D LL nee baa bhaam Note When the unit is connected to a PC or the front panel has been manually locked exposure settings can not be adjusted from the X Cite 120 PC front panel buttons Set your X Cite 120 PC to Intensity Mode by pressing the Mode button until the LED display shows 3 digits XXX The three digit number shown will indicate the iris setting as a percentage of maximum rc Warnin
50. unded cece eee ee ee ee eens sees eeeeeeaeaeeeeees 6 CD ROM X Cite 120 PC Control Panel Software amp User Guides 7 Quick Start Guide XCret20bCO e eee ee eee ee eee eaeeeaeeeeaeeaees 8 Foot Pedal Switch Aesemby eee ce cee ee eee ee ee eea ease ee ee esa eaeaeeeeeeaes 9 Cable Assembly 9 Pin RS 232 10 Hex Key 3mm lamp access cover If your packaged unit is missing any of the above components call Lumen Dynamics at 905 821 2600 or TOLL FREE 1 800 668 8752 Any additional optional items purchased to customize the unit will also be present 2 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 2 2 Front Panel LED DISPLAY LED STATUS wi MODE UP DOWN MODE UP DOWN D D A ADJUST BUTTONS X Cite SERIES 120PC Q START STOP START STOP POWER ON OFF BUTTON SWITCH Figure 1 Front Panel View 3 LUMEN DYNAMICS AL Te Fluorescence Illumination In Control 2 3 Rear Panel LIGHT AIR VENTS GUIDE PORT AC POWER ANALOG RECEPTACLE JACK INPUT SIGNAL FOOT PEDAL FUSE RS 232 DRAWER PORT Figure 2 Rear Panel View 4 LUMEN DYNAMICS OO ES gt i N X Cite Fluorescence Illumination In Control 3 Safety Information 3 1 Glossary of Symbols CAUTION risk of danger consult accompanying documents WARNING eye damage may resul
51. unit operation and to advise when certain conditions exist The following is a collective listing of messages and their meanings 1 XXXX A flashing decimal point to the right of the four numbers on the display advises the user that the unit is in Lamp Hour Mode See Section 11 1 Viewing the Accumulated Lamp Hours 2 XXX X A decimal point to the left of the furthest right digit of the four digits on the display indicates the unit is in Timer Mode The number shown will indicate the exposure time in seconds and tenth of seconds See Section 10 Timed Exposures 3 XXX Three digits on the display indicate unit is in Intensity Mode The three digit number shown will indicate the percent iris opening See Section 10 Adjusting the Light Output 4 bulb The bulb message advises the user that the bulb has failed to ignite or extinguished after striking If the bulb message appears immediately and is accompanied by an audible beeping this indicates that the lamp has not been installed correctly or that the wrong bulb type has been installed See Section 6 Installing the Lamp Module If the bulb message appears after about 25 seconds and is accompanied by an audible beeping this indicates that the lamp has failed to strike It may be a result of the lamp reaching end of life or that the lamp access cover is not secured properly in place Press the START STOP button to clear the audible alarm Power down the unit remove the lamp ac
52. use the lamp temperature to increase above eee i optimum temperature significantly reducing lamp life 6 When necessary clean the light emitting end of the light guide using an optical cleaning solution N Cleaning of unit is not required however if cleaning is desired disconnect the AC power cord from the unit and use only a water and simple detergent solution Ensure that cleaning solution does not come in contact with any optical moving mechanical or electrical parts 8 Recommended operation of the X Cite 120 is in horizontal position using the X Cite 120 beyond 15 of tilt from a horizontal position will wear lamp life 44 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 17 1 General tips to help maximize lamp lifetime 1 6 Each time the lamp is ignited the stress on the lamp effectively decreases lamp life by 5 hours therefore it is recommended to avoid shutting down the unit during lunch or short breaks or between users if being used in a multi user lab After lamp ignition avoid turning the lamp off until it has run for a minimum of 20 minutes Avoid attempting to strike a hot lamp note the X Cites Intelli Lamp will prevent this Avoid unnecessary movement and jarring of the lamp especially when it is in operation or hot When changing a lamp a Handle the lamp only by the ceramic areas b Never touch the glass envelope of the bulb inner stem the
53. vel list box Once the desired value has been selected click on the Set button to enter the value Once the Set button becomes disabled the intensity level has been entered Note If 0 is selected the shutter will not open and the error message will appear Intensity level is 0 shutter will not open If this occurs click on Cancel and reset the intensity level at 12 or greater Exposure Settings Exposure Timer Ensure that the check box next to Exposure Timer Active has been selected Exposure time can be selected by the following 2 different methods Method 1 Select exposure time by clicking on the Up Down buttons located right of the Exposure Timer The exposure time will change in increments of 1 second If a value outside of acceptable limits is chosen the error message Value is outside allowable range will appear Select Cancel and re enter a value within the allowable range Note If another value is not selected the X Cite 120 PC will automatically retain the value that was previously selected prior to the error message being displayed Method 2 Click with your mouse on the Exposure Timer list box and type in the desired value Click on the Set button once the button is disabled the selected value has been entered The allowable exposure range by this method is 0 2 to 999 9 seconds If a value outside the range is entered an error message Value is outside a
54. vironmental expert com technology aspx idCategory 2054 amp word mercury 20spill 20kits e http www alibaba com products spill kit 4 html Warning Should this X Cite 120 PC unit be used in a manner not specified by Lumen Dynamics the protection provided by the equipment may be impaired Warning The method in which lamps are disposed of must comply with local rules amp regulations for disposal of hazardous materials Lamps may be returned to Lumen Dynamics providing they are returned in their original packaging Lumen Dynamics will dispose of them in the appropriate manner Caution The lamp modules operational life can be significantly shortened if it is handled incorrectly Do not touch the bulb s glass envelope or the inside surface of the reflector Skin oils can cause the lamp module to fail prematurely Caution Prior to opening the unit and handling the lamp module allow the lamp module to cool down completely Caution Any electronic equipment connected to the X Cite 120 PC must be IEC950 certified Cleaning Clean exterior of the unit with a water dampened cloth and simple detergent only 7 LUMEN DYNAMICS X Cite Fluorescence Illumination In Control 4 installing the Lamp Module pm ei pn EE Note Refer to Section 3 Safety Precautions before proceeding j gt 4 1 1 Be sure the AC power cord is disconnected from the unit 4 1 2 Remove the screw from the top of the lamp acces
55. w or overhead room light DO NOT use an X Cite or any other focused light source for this test 39 LUMEN DYNAMICS x Cite Fluorescence Illumination In Control 3 Look at the quartz at the other end of the LLG a Bubble free quartz end will appear as a bright solid circle you may also be able to see a thin circular outline at the quartz liquid interface Bubbles at near the quartz end appear as dark spots as small as 0 5mm in diameter or even as larger more defined spheres Bubbles in the middle of the light guide may not be well defined spots but will appear as dark shadows In extreme cases where the bubble is blocking the entire diameter of the light guide no light will come through even when pointing the distal end at a light source 100 11 3 5 2 e Appearance and output of light guides with bubbles relative to an LLG without bubbles 100 Can a bubbled light guide recover 1 Yes light guides with small bubbles can sometimes recover Disconnect the light guide from the X Cite unit and leave the light guide undisturbed on a shelf for 1 2 weeks For this to be effective it is important to catch the bubble when it is small When should light guides be replaced 1 Itis usually time to replace a light guide when Illumination is low and replacing the lamp does not improve brightness Dark or uneven areas become visible in the field of view a bubble is blocking part of the light

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