MBS - User Manual - 2011-09 - MBS
Contents
1. io SS O las 1 3 2 Coliforms survey CO A02 Rod shaped aerobic microorganisms Gram negative non spore forming cytochrome oxidase negative fermenting lactose with production of acids in the presence of bile salts or other surfactants ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE CO A02 Coliforms MBS MICRO BIOLOGICAL SURVEY If C COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS VI NEGATIVE fee COLOR OF ANALYSIS AT END 24 HOURS TIME OF COLOR CHANGE lt 3 30 12 30 16 30 19 00 22 00 gt 24 00 L x time in hours coma CIEZEZE ZAR Gc CONTAMINATION a si M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 14 of 18 i MBS HACCP amp ACQUE EASY TEST ser USER MANUAL BASIC SYSTEM SURVEY 2011 SEPTEMBER 1 3 3 E coli survey CO A02 Rod shaped aerobic Gram negative non spore forming cytochrome oxidase negative microorganism fermenting lactose with production of acids in the presence of bile salts or other surfactants at temperature of 44 C produce indole from tryptophan ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE CO A02 E coli MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS NEGATIVE COLOR OF ANALYSIS AT END 30 HOURS TIME OF COLOR CHANGE lt2. 2 00 2 00 930 1300 1800 22 00 26 00 30 00 ENAN 0 minute x time in hours oe pete fete lwo le CFU o CFUmI T 7 CONTAMINATION i 4 Z i all 3 0024 x 1 3 4 Enterobacteriaceae survey EB A04 Gram negative microorganisms aerobic anaerobic facultative non spore forming oxidase negative ferment glucose and lactose with gas production reduce nitrate and are negative for oxidase test ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE EB A03 Enterobactenaceae MBS MICRO BIOLOGICAL SURVEY cf fi COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS TIME OF ANALYSIS TIME OF COLOR CHANGE Med i 10 00 13 00 16 00 19 00 2200 gt 2400 Bile i nianti me in hours ee ee e pw pw pw a e mec am CONTAMINATION i PE rue gt to wot ow ow om ot o Cruel 105 924 40 1 3 5 Staphylococcus aureus survey SP A04 Gram positive cocci coagulase positive catalase positive fixed non spore forming facultative anaerobes fermenting mannitol and osmotolerant ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE SP A04 Staphylococcus aureus MBS MICRO BIOLOGICAL SURVEY re of C COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS POSITIVE NEGATME COLOR OF ANALYSIS AT END 48 HOURS TIME OF COLOR i CHANGE 11 00 2000 2930 3600 4330 46 00 gt 4800 a hO minute x time in hours E De be e De R ti CONTAMINATION S F 2 CFUicm g
3. 60 00 DE 0 minute x time in hours ACI e LALA ESE ZEN ENEA TEC Rar apa ai iii 107 10 10 CFU cm 10 8 9 142 1 100 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 17 of 18 i MBS HACCP amp ACQUE EASY TEST ser USER MANUAL BASIC SYSTEM SURVEY 2011 SEPTEMBER 1 3 12 Clostridium perfringens survey CP A17 Obligate anaerobic Gram positive microorganism without cytochromes able to form spores catalase and peroxidase negative Can fix nitrogen produce the cap and is mild proteolytic makes the butyric fermentation ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE CP A17 Closindium perfringens MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAAIMUM TIME OF ANALYSIS POSITIVE NEGATIVE COLOR OF ANALYSIS AT END 36 HOURS TIME OF COLOR i hG minute x time in hours CONTAMINATION i i a z IcFU em gt 10 10 10 10 10 1 CFU cm 107 922 1 100 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 18 of 18
4. EMBER 1 1 Introduction Dear User thank you for purchasing MBS HACCP amp ACQUE EASY TEST an innovative rapid colorimetric system to perform microbiological tests on food water and surfaces developed in collaboration with Roma Tre University The method of analysis is based on the observation of the color change in the Suspension formed in the analysis vial used when the test sample is added the suspension changes color turns if there are microorganisms the greater the amount of microorganisms the more rapid is the change of color The main features of the MBS HACCP amp ACQUE EASY TEST are Speed analysis time from preparation to the achievement of results from 2 to 5 times less than traditional methods Ease of use Anyone anywhere can do the analysis without the need for other reagents or special equipment Sensitivity you can detect even a single microorganism present in the sample Selectivity it can detect different species of microbial organisms to the experimental limit of 99 999 Cost The cost of each analysis turns out to be 2 to 4 times cheaper than traditional methods The MBS method has been validated according to ISO 16140 2003 Microbiology of food and animal feeding stuffs Protocol for the validation of alternative methods M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net in
5. ETHOD INCUBATION TEMPERATURE EF A09 Enterococcus faecalis MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS NEGATIVE COLOR OF ANALYSIS AT END 24 HOURS TIME OF COLOR j CHANGE lt 430 430 730 10 00 13 00 16 00 1900 gt 24 00 AIA x Sa x time in hours E Dw fe e e R ie CONTAMINATION 1 3 10 Aspergillus spp survey AS A10 Multicellular eukaryotic heterotrophic highly aerobic organisms growing in carbon rich substrates resistant to high concentrations of antibiotics and sulfonamides ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE AS A10 Aspergillus spp MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS COLOR OF ANALYSIS AT END 160 HOURS TIME OF COLOR CHANGE 0 72 00 72 00 150 00 gt 160 00 EQUATION CONTAMINATION n 8 4 CONTAMINATION gt 104 105 105 1 3 11 Saccharomyces spp survey SC A11 Unicellular eukaryotic heterotrophic highly aerobic organisms growing in carbon rich substrates resistant to high concentrations of antibiotics and sulfonamides ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE SC A11 Saccharomyces spp MBS MICRO BIOLOGICAL SURVEY 30 C COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS POSITIVE NEGATIVE j COLOR OF ANALYSIS AT END Ei l 60 HOURS l TIME OF COLOR CHANGE lt 600 600 13 00 20 30 27 30 35 00 4200 50 00
6. It also differs by type of sample to be analyzed solid sample liquid sample or surface analysis M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 5 of 18 i MBS HACCP amp ACQUE EASY TEST soe USER MANUAL BASIC SYSTEM chee 2011 SEPTEMBER 1 2 1 Solid sample Step 1 Opening e Open the vial taking care to flip the cap so that the inner surface does not come into contact with the surface to avoid contamination Step 2 Inserting the sample e Take a small aliquot of the food no bigger than a grain of maize corresponding to approximately 1 g with a tool used during the processing of the food itself and insert it into the vial alternatively you can use sterile tweezers Note the size or the exact weight of the sample to be examined is not important Infact the statistical variability microbiological examination of food samples is such that no method of analysis including the reference method has a variability of less than 60 70 Therefore the analysis has the same intrinsic value if it fits into a 1 g vial 2 g or 0 5 g This variability is due both to the method of analysis and to the sample itself since these microorganisms often grow in grouped colonies and therefore are not dispersed evenly in the middle Note for inserting the sample into the vial we re
7. MBS MICRO BIOLOGICAL SURVEY MBS HACCP amp ACQUE EASY TEST USER MANUAL BASIC SYSTEM 2011 SEPTEMBER MBS HACCP amp ACQUE EASY TEST BASIC SYSTEM MBS MULTI READER USER MANUAL M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net i MBS HACCP amp ACQUE EASY TEST soe USER MANUAL BASIC SYSTEM sn 2011 SEPTEMBER TABLE OF CONTENTS 1 1 Introduction 3 1 2 How to use 5 1 2 1 Solid sample 6 1 2 2 Liquid sample 9 1 2 3 Surface analysis 11 1 3 Color scales and correlation tables 14 1 3 1 Total Viable Count CBT A01 14 1 3 2 Coliforms survey CO A02 14 1 3 3 E coli survey CO A02 15 1 3 4 Enterobacteriaceae survey EB A04 15 1 3 5 Staphylococcus aureus survey SP A04 15 1 3 6 Pseudomonas aeruginosa survey PAO A05 16 1 3 7 Salmonella spp survey SL A06 16 1 3 8 Listeria spp survey LY A07 16 1 3 9 Enterococcus faecalis survey EF A09 17 1 3 10 Aspergillus spp survey AS A10 17 1 3 11 Saccharomyces spp survey SC A11 17 1 3 12 Clostridium perfringens survey CP A17 18 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 2 of 18 rt MBS HACCP amp ACQUE EASY TEST apc USER MANUAL BASIC SYSTEM sis 2011 SEPT
8. an perform a test to confirm the presence of E coll adding 3 5 drops of Kovacs reagent to the vial at the end of the analysis before the sterilization If there is E coli within a few seconds you will see the development of a small surface layer of purple red liquid Note to make the test for confirmation of E coli open the vial carefully avoiding contamination with the liquid contained in it because if the color is tacked the vial will contain a significant number of bacteria and it is therefore preferable to use disposable gloves even non sterile for the operation of opening the vial If you come in contact with the liquid wash the affected area immediately and thoroughly with antibacterial soap or normal soap and then with a disinfectant M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 12 of 18 i MBS HACCP amp ACQUE EASY TEST oer USER MANUAL BASIC SYSTEM sinas 2011 SEPTEMBER Step 5 Post analysis sterilization e Without opening the vial firmly press the top of the cap and shake for about 10 seconds The addition of the sterilizing agent can cause a color change After 5 10 minutes the contents of the vial is completely sterilized Note with the sterilization of the microorganisms are destroyed the reagents are rendered inert and the vial can be safely dispo
9. cceptable bacterial load in the sample the generally accepted limits for the type of sample taken by law are shown by way of illustration on the Quality Control Sheets e Compare the color of the vial with the correlation table set out in paragraph 1 3 or in Quality Control Sheet e lf you have started the compilation of the Quality Control Sheet fill it in with the result of the analysis e n the case of analysis for detection of E coli and only if the result is positive bright yellow vial you can perform a test to confirm the presence of E coll adding 3 5 drops of Kovacs reagent to the vial at the end of the analysis before the sterilization If there is E coli within a few seconds you will see the development of a small surface layer of purple red liquid Note to make the test for confirmation of E coli open the vial carefully avoiding contamination with the liquid contained in it because if the color is tacked the vial will contain a significant number of bacteria and it is therefore preferable to use disposable gloves even non sterile for the operation of opening the vial If you come in contact M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 7 of 18 i MBS HACCP amp ACQUE EASY TEST oer USER MANUAL BASIC SYSTEM sinas 2011 SEPTEMBER with the liquid wash t
10. commend using a tool used during the processing of the food itself since by so doing you will be able to detect any contamination of the food due to extrinsic causes Step 3 Start analysis e Open the vial of water supplied with the reaction vial and insert the entire contents of the vial itself e Mix by inverting the vial several times to completely M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 6 of 18 uf MBS HACCP amp ACQUE EASY TEST sE USER MANUAL BASIC SYSTEM sve 2011 SEPTEMBER dissolve the reagent twenty seconds e f necessary fill in the Quality Control Sheet e Place the vial in the incubator thermostat Note the vial grows 2 liquid phases the upper one consisting of white transparent oil does not change during the analysis formed at the bottom of the vial from the water with which the reagent is dissolved The color of the second phase will develop fully within 15 20 minutes from the dissolution of the reagent and that is what must be observed for the evaluation of the results of the analysis Step 4 Control of the analysis results e You only need to check the color of the vial once after a set number of hours depending on the type of analysis This number of hours does not depend only on the type of analysis but includes the operational limit of a
11. fo emmebiesse net page 3 of 18 MICRO BIOLOGICAL MBS HACCP amp ACQUE EASY TEST USER MANUAL BASIC SYSTEM 2011 SEPTEMBER Available reagents for the selective search of the following microorganisms 9 cd Sa eS N o Total Viable Count CBT A01 Coliforms Totals ed E coli CO A02 Enterobacteriaceae EB A03 Staphylococcus aureus SP A04 Pseudomonas aeruginosa PAO A05 Salmonella spp SL A06 Listeria spp LY A07 Enterococcus faecalis EF A09 Molds Aspergillus spp AS A10 10 Yeasts Saccharomyces spp SC A11 11 Clostridium perfringens CP A17 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 4 of 18 TL MBS HACCP amp ACQUE EASY TEST ie USER MANUAL BASIC SYSTEM BIOLOGICAL SURVEY 2011 SEPTEMBER 1 2 How to use The kit comes in a pack containing all the material for the analysis the reaction vial vial and a vial of distilled water vial of water To perform the analysis you must also have a bacteriology thermostatic incubator programmable to 30 37 or 44 Before handling the vials and proceeding with the analysis a thorough hand washing is recommended The performance analysis can be summarized into 5 phases opening insertion of the sample early analysis order analysis and sterilization
12. he affected area immediately and thoroughly with antibacterial soap or normal soap and then with a disinfectant Step 5 Post analysis sterilization e Without opening the vial firmly press the top of the cap and shake for about 10 seconds The addition of the sterilizing agent can cause a color change After 5 10 minutes the contents of the vial are completely sterilized Note when the sterilization of the microorganisms are destroyed the reagents are rendered inert and the vial can be safely disposed of as a sanitary waste non hazardous according to the D M of 25 5 89 Such waste after sterilization can be disposed of in the same way as drugs which have expired M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 8 of 18 i MBS HACCP amp ACQUE EASY TEST oer USER MANUAL BASIC SYSTEM Sas 2011 SEPTEMBER 1 2 2 Liquid sample Step 1 Opening e Open the vial taking care to flip the cap so that the inner surface does not come into contact with the surface to avoid contamination Step 2 Inserting the sample e Using a pasteur dropper sterile disposable supplied on request draw a quantity of fluid to examine and introduce about 1 ml of that liquid into the vial Step 3 Start analysis e Open the vial of water supplied with the reaction vial and insert the ent
13. in hours R ERE E HE CFUlg Ha 10 98 0219 CONTAMINATION i 1 3 8 Listeria spp survey LY A07 Gram positive non spore forming facultative anaerobic microorganisms resistant to many antibiotics Grow at pH between 5 and 9 and in the presence of NaCl to 10 Catalase positive oxidase negative do not hydrolyze urea gelatin and casein Do not reduce nitrates do not produce indole and hydrogen sulfide ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE LY AD7 Listeria spp MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS POSTIVE O E NEGATIVE COLOR OF ANALYSIS AT END i 36 HOURS TIME OF COLOR cr ERF 1200 1630 2030 2500 2900 33 00 gt 3600 oe ho minute x time in hours fervor gt A 20 20 100 1 10 1 0 eas crum 1007 22 CONTAMINATION cruenti gt ot ow wm wo 1 0 rad 107 9225100 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 16 of 18 i MBS HACCP amp ACQUE EASY TEST oer USER MANUAL BASIC SYSTEM SURVEY 2011 SEPTEMBER 1 3 9 Enterococcus faecalis survey EF A09 Gram positive fixed facultative anaerobes non hemolytic catalase negative microorganism fermenting glucose without gas production and hydrolyze esculin ANALYSIS CODE ANALYSIS NAME ANALYTICAL M
14. ire contents of the vial itself e Mix by inverting the vial several times to completely dissolve the reagent twenty seconds e f necessary fill in the Quality Control Sheet e Place the vial in the incubator thermostat Note the vial grow in 2 liquid phases the upper one consisting of white oil transparent does not change during the analysis formed at the bottom of the vial from the water with which the reagent is dissolved The color of the second phase will develop fully within 15 20 minutes from the dissolution of the reagent and that is what must be observed for the evaluation of the results of the analysis Step 4 Control of the analysis result e You only need to check the color of the vial once after a set number of hours depending on the type of analysis This number of hours does not depend only on the type of analysis but includes the operational limit of acceptable bacterial load in the sample the generally accepted limits for the type of sample taken by M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 9 of 18 i MBS HACCP amp ACQUE EASY TEST oer USER MANUAL BASIC SYSTEM sinas 2011 SEPTEMBER law are shown by way of illustration on the Quality Control Sheets e Compare the color of the vial with the correlation table set out in paragraph 1 3 or in Qual
15. ity Control Sheet e l you have started the compilation of the Quality Control Sheet fill it in with the result of the analysis e In the case of analysis for detection of E coli and only if the results are positive bright yellow vial you can perform a test to confirm the presence of E coli adding 3 5 drops of Kovacs reagent to the vial at the end of the analysis before the sterilization If there is E coli within a few seconds you will see the development of a small surface layer of purple red liquid Note to make the test for confirmation of E coli open the vial carefully avoiding contamination with the liquid contained in it because if the color is tacked the vial will contain a significant number of bacteria and it is therefore preferable to use disposable gloves even non sterile for the operation of opening the vial If you come in contact with the liquid wash the affected area immediately and thoroughly with antibacterial soap or normal soap and then with a disinfectant Step 5 Post analysis sterilization e Without opening the vial firmly press the top of the cap and shake for about 10 seconds The addition of the sterilizing agent can cause a color change After 5 10 minutes the contents of the vial are completely sterilized Note when the sterilization of the microorganisms are destroyed the reagents are rendered inert and the vial can be safely disposed of as a sanitary waste non hazardous according
16. olo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 11 of 18 uf MBS HACCP amp ACQUE EASY TEST sE USER MANUAL BASIC SYSTEM sve 2011 SEPTEMBER Note the vial will grow in 2 liquid phases the upper one consisting of white transparent oil does not change during the analysis formed at the bottom of the vial from the water with which the reagent is dissolved The color of the second phase will develop fully within 15 20 minutes from the dissolution of the reagent and that is what must be observed for the evaluation of the results of the analysis Step 4 Control of the analysis result e You only need to check the color of the vial only once after a set number of hours depending on the type of analysis This number of hours does not depend only on the type of analysis but includes the operational limit of acceptable bacterial load in the sample the generally accepted limits for the type of sample taken by law are shown by way of illustration on the Quality Control Sheet e Compare the color of the vial with the correlation table set out in paragraph 1 3 or in Quality Control Sheet e lf you have started the compilation of the Quality Control Sheet fill it in with the result of the analysis e n the case of analysis for detection of E coli and only if the result is positive bright yellow vial you c
17. sed of as a sanitary waste non hazardous according to the D M of 25 5 89 Such waste after sterilization can be disposed of in the same way as drugs which have expired M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 13 of 18 i MBS HACCP amp ACQUE EASY TEST oer USER MANUAL BASIC SYSTEM SURVEY 2011 SEPTEMBER 1 3 Color scales and correlation tables As required by law the concentration of bacteria is expressed in CFU g or CFU ml or CFU cm Colony Forming Units respectively for the analysis of solid or liquid samples or surfaces by analogy with the results expressed with the traditional analysis even if the MBS method is based on the detection of bacterial metabolism and not on the replication of microorganisms The credits approximately correspond to individual bacterial cells 1 3 1 Total Viable Count CBT A01 Detection of aerobic or microaerophilic mesophilic microorganisms able to grow on complete media ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE CET A01 Total Viable Count MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS COLOR OF ANALYSIS AT END 24 HOURS TIME OF COLOR Fi ka ee x time in hours a or mi CFU g o CFU ml 10 74032 CONTAMINATION s i crue gt wot we we mo ot
18. t 10 10 ka CFU cm 10 5 5 0 5 47 100 M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 15 of 18 i MBS HACCP amp ACQUE EASY TEST ser USER MANUAL BASIC SYSTEM SURVEY 2011 SEPTEMBER 1 3 6 Pseudomonas aeruginosa survey PAO A05 Moveable non spore forming microorganism rod shaped Gram negative aerobic anaerobic facultative cytochrome oxidase and catalase positive produce pyocyanine ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE PAQ A05 Pseudomonas aeruginosa MBS MICRO BIOLOGICAL SURVEY COLOR OF ANALYSIS AT START MAXIMUM TIME OF ANALYSIS POSITIVE NEGATIVE COLOR OF ANALYSIS AT END 24 HOURS TIME OF COLOR ina kann ang bas lans gian K i hO minute x time in hours CONTAMINATION z TE run gt 0 oe ow Poo tf 00 Kia 10 75 92 100 1 3 7 Salmonella spp survey SL A06 Gram negative aerobic anaerobic facultative enterobacteria able to ferment mannitol Catalase positive produce hydrogen sulfide reduce nitrate to nitrite ANALYSIS CODE ANALYSIS NAME ANALYTICAL METHOD INCUBATION TEMPERATURE SL A06 Salmonella spp MBS MICRO BIOLOGICAL SURVEY af C COLOR OF ANALYSIS AT START MAAIMUM TIME OF ANALYSIS POSITIVE NEGATIVE COLOR OF ANALYSIS AT END 32 HOURS TIME OF COLOR EQ hG minute x time
19. to the D M of 25 5 89 Such waste after sterilization can be disposed of in the same way as drugs which have expired M B S Srl Polo Tecnologico Tiburtino Via Giacomo Peroni 386 00131 Roma C F e P I 09423051003 tel 39 06 40040358 fax 39 06 40040364 www emmebiesse net info emmebiesse net page 10 of 18 i MBS HACCP amp ACQUE EASY TEST oer USER MANUAL BASIC SYSTEM SURVEY 2011 SEPTEMBER 1 2 3 Surface analysis Step 1 Preparation of the reaction vial e Open the vial of water supplied with the vial for analysis and insert the entire contents of the reaction vial itself e Mix by inverting the vial several times to completely dissolve the reagent twenty seconds Note after dissolution of the reagent it is advisable to unscrew the reaction vial cap of analysis to facilitate subsequent operations Step 2 Sampling e Open the cotton swab vial containing the neutralizing solution e Rub the swab on the surface trying to cover a square area of about 10 cm per side Note do not re insert the swab into the vial containing the neutralizing solution Step 3 Start analysis e Open the previously prepared reaction vial e Insert the swab into reaction vial e f necessary fill in the Quality Control Sheet e Place the vial in the incubator thermostat Note you can reseal the vial containing the neutralizing solution with the cap of reaction vial before disposal M B S Srl P
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