Lx 400 Phase Contrast Kit
Contents
1. and place the actual eye piece in eye tube Put specimen slide on stage Microscope is now ready for phase contrast observation Repeat the entire procedure when observing through 20X 40X and 100X phase objectives and make sure that corresponding phase annulus is to be selected for that particular phase objective CARE AND MAINTENANCE Loose particles can be removed from the surface of the condenser diaphragm or phase annulus by gently wiping them with lens tissue dampened with Acetone or Methanol It is better to avoid leaving finger prints on the surface of condenser phase annulus When not in use for long time it is recommended to store the phase contrast kit in its original packaging Our policy is one of continuous development Labo America Inc reserves the right to change design and specifications without prior notice Labo America Inc U S A 920 Auburn Court Telephone 510 445 1257 Fremont Fax 510 445 1317 CA 94538 sales laboamerica com LABOMED and Lx 400 are registered trademarks of Labo America Inc With a policy of continuous development Labo America Inc reserves the right to change design and specifications without prior notice ISO 9001 2000 2009 Labo America Inc 9126000 990A 12 2009 File No A9020 Printed in U S A2. LABO M E D ideas for Vision To ensure proper use of this instrument as well as to avoid injury while operating instrument understanding this manual completely before use is highly recommended www laboamerica com INTRODUCTION Phase contrast is the method used in light microscopy and was developed on the principle that if you speed up a direct light path you can cause destructive interferences patterns in a viewed image These patterns make details in the image appear darker against a light background To cause these interferences patterns a system of rings located both in the objective lens and in the condenser system must overlap When aligned correctly light waves emiited from a light source reach your cornea one half of the wavelength out of phase resulting in a specimen with details that are greatly enhanced Phase contrasting is only useful on the specimen that is colorless as well as transparent in nature and usually difficult to distinguish from its surroundings Such specimens are referred to as Phase objects Examples of phase objects include cell parts in protozoans bacteria sperm tails and other types of unstained cells PACKING LIST Lx 400 Phase Contrast Kit 9126000 RP PH 10x RP PH 20x RP PH 40x SL RP PH 100x SL Oil 5 1 Phase Annulus Turret 3134045 2 Centering Telescopic Eyepiece 3126005 3 Adapter for Telescopic Eyepiece 9126005 10 4 Green Filter 3126065 5 Phas
3. e Contrast Objectives 10X 9123010 20X 9123020 40X 9123040 100X 9123100 Pleas make sure all contents of the Phase Contrast Kit are intact If any physical damage is visible please contact your local distributor INSTALLATION Loosen the thumb screw 1 to remove the condenser Replace the condenser with the new Abbe condenser provided in the phase kit Remove the condenser from its position by loosening the two thumbscrews on both sides of the condenser as shown in Fig 1 and 2 Place the phase annulus turret in position from where the condenser has just been removed so microscope looks like that in Fig 3 Install all phase contrast objectives in their respective positions The long push in to engage thumbscrews on both sides of the phase turret are provided for proper alignment of the phase ring By disengaging these screws the phase turret can be adjusted at any desired phase annulus 10X 20X 40X amp 100X by turning the thumbwheel to the desired position There is also an 0 setting on the thumbwheel for bright field application CALIBRATING THE INSTRUMENT Turn ON the microscope illumination system Close the diaphragm of Koehlar illumination to minimum position and bring phase turret at O position Engage 10X phase contrast objective in field Looking through the normal eyepiece a bright circular field will appear in FOV Center the condenser of phase turret by using two thumbscrews so that the bri
4. ght field is in the center of field Keeping the 10X phase objective in field open the condenser diaphragm Put any specimen slide on mechanical stage and focus the 10X phase objective on specimen keeping the phase turret at 0 position Remove the eyepiece and insert the centering telescopic eyepiece in one of the eye tube of binocular head if the eye tube ID is 30mm then an adapter provided with kit is to be used with telescopic eyepiece Loosen the thumbscrew of the telescopic eyepiece While looking into the telescopic eyepiece extend the front lens until the dark ring of 10X objective in the field of view becomes a very sharp and crisp image The ring should not appear fuzzy Tighten thumbscrew and the image will remain in focus Fig 1 Py od Jz 4 CALIBRATING THE INSTRUMENT Disengage the thumbscrews of the phase turret and bring 10X phase annulus in field by turning the thumbwheel 10X will appear on thumbwheel Push in thumbscrews to lock the position While looking through the telescopic eye piece two rings will be visible one dark and other bright They may not overlap By turning centering adjustment thumbscrews of the condenser align the rings so that they overlap each other as shown below Place the green filter on koehler illumination system remove the centering telescopic eye piece
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