Heraeus Multifuge 1S - Frank`s Hospital Workshop
Contents
1. 37 Actuatingithe id 5 corsario 37 Opening the NG 2 oa ata cea acea ba ea 37 Closing MM ceci ace aaa ate bt ace 37 Installing the rotor nenea 38 Loading the rotor eee 39 Maximum loading aaa 39 Filling the centrifuge tubes 40 Maximum permissible load difference 40 Inserting the centrifuge tubes 41 Entering parameters eee eee 43 Deceleration curves i 43 Switching from speed to RCF display 43 Bucket selection for swinging bucket rotors 43 Selecting Speed cae ca aa aia atata 44 Entering the RCF value ii 44 More about the RCF value 45 Selecting run time u eee eee 45 Run time Selection ii 45 Continuous operation e 46 Extended time mode eee 46 Selecting the temperature 47 Pretemp function keke 47 Starting the centrifuge i 48 Contents Imbalance display Ei k 48 Changing the settings during the run 48 Stopping the centrifuge a 49 Stopping with preset run time 49 Stopping with continuous operation 49 Temperature control during standby2. 2 fe 60 90 120 time s 150 180 210 240 83 16000 LI E M TEHTE time s speed rpm Deceleration profiles Micro Liter Rotor 48 x 2 ml 75003348 16000 14000 12000 10000 8000 6000 4000 2000 0 30 60 90 120 150 180 210 240 270 e time s 85 86 Speed RCF diagrams Nmax max speed Speed RCF diagram Swinging Bucket Rotor TTH 400 75002000 10000 Round bucket Rectangular bucket Nmax 4700 rpm 1000 RCF max 4618 REF x g 100 1000 speed rpm 100 10000 87 Speed RCF diagram BIOshield 600 Rotor 75002005 10000 Nmax 6000 rpm RCF max Nmax 6842 1000 RCF x g 100 1000 10000 speed rpm 100 88 RCF x g Speed RCF diagram Micro plate carrier MP 3300 75002010 10000 Nmax 4400 rpm RCF max Nmax 3355 1000 100 100 1000 speed rpm 10000 89 Speed RCF diagram Fixed Angle Rotor FA12 94 Highconic 7500 2006 100000 Nmax 10 350 rpm fmax 12 5 cm RCF Tmax Nmax 15 090 fmin 6 1 cm 10000
3. 49 Working with programS i 50 Program display i 50 Entering changing a program 50 Centrifuging with a program 50 QUICK RUN Qta iaia 51 Removing the rotor i lula kanala anak s 51 Audible alarm iaia 52 Turning off the centrifuge 52 Maintenance and care 53 Maintenance to be performed by the customer 53 CIS AMI G sixes DD aa ca da aa nat ata 53 Disinfectiani ee ceea at atacul ace bea e a aaa 54 Decontamination ii 56 Autoclavingi uniti laici 56 The KENDRO service offer 57 Warranty conditions i 57 Troubleshooting 11 xxx 59 Emergency lid release i 59 Error troubleshooting nenea 61 Contacting Kendro Service 70 Technical Data ici 71 Electrical connections fuses 73 Appendix sica 75 Acceleration and deceleration profiles 75 Speed RCF diagramSs ii 87 e GP DD DD DD alain 93 For your safety For your safety Heraeus centrifuges are manufactured according to current technical standards and regulations Nonethe less centrifuges may pose danger to individuals and surrounding if e they are not used as designed e they are operated by untrained perso
4. 9 fastest permanent display acceleration profile last set 1 9 Deceleration profiles 1 slowest 9 fastest permanent display deceleration profile last set 1 9 Speed RCF run current value of speed or RCF after activation of switch key end End lid open OPEN Lift Lid if lid is not automatically lifted off before start lid closed 0 with flashing point rotor not yet identified error code will flash in display Run time time selection remaining run time to 0 continuous operation hld run time passed in hours minutes quick run run time passed as long as button is held in minutes and seconds Temperature run current sample temperature in C in temperature equilibrium only for instruments with refrigeration unit Keys start normal start of the centrifuge stop manual stop of a run open lid open lid possible only with the instrument switched on quick run short term operation of the centrifuge as long as key remains pressed rpm RCF switch switching between rpm and RCF display bucket set Setting of the bucket number pretemp Pre temp function set keys stepwise increase decrease of setpoint values Short pressing of any of the set keys switch from current to preset value signaled by flashing display Error codes troubleshooting see chapter Troubleshooting E 19 Unpermissible rotor E 20 Rotor not identified E 29 Motor or rotor bloc
5. Symptom Possible causes and corrective measures Instrument does not start or No rotor present or rotor identification impossible decelerates to standstill A Check if a certified rotor is inserted B Following a brief power failure the rotor could not be identified Switch the instrument off and on again using the main switch Rotor stops without Error in speed measurement deceleration to standstill Switch the instrument off and on again If the error persists Instrument cannot be contact the KENDRO service station operated Instrument cannot be Wrong status information from lid lock operated Switch the instrument off and on again If the error persists contact the KENDRO service station Instrument decelerates Motor runs in wrong rotational direction after a short starting up Switch the instrument off and on again If the error persists contact the KENDRO service station 66 Troubleshooting Symptom Possible causes and corrective measures Motor does not start Motor or rotor blocked 1 Switch instrument off and on again using the main switch 2 Open the lid 3 Check whether the rotor can turn freely If you cannot clear the malfunction contact the KENDRO service station Rotor stops without Control voltage breaks down deceleration to standstill or Switch the instrument off and on again If the error persists does not start contact the KENDRO service station Rotor stops without
6. Troubleshooting Error troubleshooting E If problems other than those described in the following tables arise you must contact KENDRO service Symptom Possible causes and corrective measures Displays remain dark The drive stops Mains voltage disconnection The rotor stops without 1 ls the mains plug turned on deceleration The lid 2 Check the main connection cannot be opened 3 If the main connection is ok contact the nearest KENDRO service station Displays fail briefly The drive stops suddenly Main connection was briefly interrupted The rotor stops without 1 Turn off main switch deceleration 2 Check whether the mains power cord is connected properly 3 Restart the centrifuge Lid cannot be opened Pressing the open lid key Lid not correctly engaged or lid warped has no effect Check if main connection is working and the instrument is switched on display is lit Press the lid down in the middle of the front section once and press the open lid key If this is unsuccessful you may open the lid using the emergency lid release see page 59 61 Troubleshooting Symptom Possible causes and corrective measures Centrifuge is exceptionally Imbalance noisy 1 Stop the centrifuge by pressing the stop key in case of emergency unplug mains power cord 2 Wait until the centrifuge comes to a complete stop 3 Check whether the rotor is properly loaded 4 Check whether a
7. memory and remains unchanged until changed by a new entry Switching from speed to RCF display Upon turning on the centrifuge the speed display is set Use the speed mode selection key E to switch the speed entry and display between rpm and RCF Bucket selection for swinging bucket rotors When running a swinging bucket rotor the automatic rotor identification feature will recognize the rotor body Depending on the bucket or carrier installed on the rotor body the corresponding type of bucket or carrier must be selected on the bucket selection function The bucket selection affects the correct RCF values display and the correct selection of the corresponding parame ters of the temperature regulation The current part number of the buckets is displayed by pressing the bucket selection key corresponds to the last four 4 digits of the order number 5 F F _ 5 To change the bucket pa selection press the L g i G bucket selection key again until the correct set of buckets appears The value is accepted when the change back to the speed RCF display has occurred 43 Operation Selecting speed The centrifuge speed can be set to a minimum of 300 rpm and to a maximum of 15 000 rpm depending on the rotor You can adjust the speed in 10 rpm increments Pro ceed as follows 1 By pressing the set keys lA once for an increase or DA for a decrease in the speed section of the control panel
8. set keys A for an increase or MI for a decrease in the temperature section of the control panel once to switch from the actual to the set point mode 2 By briefly pressing the input key you can now raise i or lower the run time in 1 steps 3 If you keep the selected key pressed the display changes continuously slowly at first and then at ac celerated paces up or down 4 Release the key as soon as you have reached the desired value and adjust if necessary by repeatedly pressing the key The temperature display flashes for a number of sec onds then changes to the current value display The temperature set point is now stored Pretemp function The Pretemp function permits easy and quick pre temperature regulation of the empty rotor Upon calling this function by pressing the key E all you have to do is enter the desired temperature After actuating the start key zi the rotor is pre tempered with an optimum rotational speed set by the pre temperature function The actually achieved temperature change in the pre temperature run time depends on the rotor used and the ambient temperature Occasionally multiple pre temperature runs are necessary to achieve the desired rotor temperature 47 Operation Starting the centrifuge Once the rotor is properly installed the main switch is turned on and the lid is closed you can start the centri fuge Press the st
9. 1 25x112 1 29x118 1 16 5x120 4 30x17 14 ira ci 27 Rotors and accessories Tabelle 3 Adapter 5 Accessories for No of Micro plate carrier MP 3300 Sider 7500 2010 Aerosol tight cap 2 pieces incl seals and lubricants 7500 2011 Adapter for micro plate carrier 7500 2013 2 pieces incl lubricants 7500 2012 Table 3 Adapter 6 Max tube Tube capacity Number Color No of Adapter for dimensions ml per set order Micro Liter Rotor 7500 3348 d x length mm 7600 3750 Reduction sleeve PCR 6 2 x 20 Reduction sleeve 8 x 43 5 0 5 0 6 Turquoise 7600 3758 Reduction sleeve 0 25 0 4 Red 76003759 d diameter 28 Rotors and accessories Handling the rotor Swinging Bucket Rotor TTH 400 BlOshield 600 Rotor Micro plate carrier All positions must always be loaded with identical carrier buckets E The various swinging buckets are split up into weight categories These can be identified through the letters suffixing the order number on the bucket Buckets of identical weight categories should always be installed in opposing rotor positions to avoid imbalance E In case of repeat orders of buckets and carriers please indicate the present weight category The swinging bucket rotors have a slide coating which guarantees perfect operation without additional lubrica tion of body trunnion pins for many years Should an imb
10. Ir Ir 30 60 90 120 150 time s 180 210 speed rpm 5000 4000 3000 2000 1000 Deceleration profiles Swinging Bucket Rotor TTH 400 75002000 60 90 120 150 time s 180 210 77 78 speed rpm 6000 5000 4000 3000 2000 1000 Acceleration profiles BlOshield 600 Rotor 75002005 ML Ab VAI EE AL fy Ay VL AA 2z 30 60 90 120 time s 150 180 210 240 speed rpm Deceleration profiles BlOshield 600 Rotor 75002005 6000 5000 4000 3000 2000 1000 time s 79 80 speed rpm 4000 3000 2000 1000 Acceleration profiles Micro plate carrier MP 3300 75002010 BIA AUA 30 60 90 time s 120 150 180 210 speed rpm Deceleration profiles Micro plate carrier MP 3300 75002010 4000 3000 2000 1000 0 30 60 90 120 150 180 E time s 81 82 speed rpm 11000 10000 9000 8000 7000 6000 5000 4000 3000 2000 1000 Acceleration profiles Fixed Angle Rotor FA12 94 Highconic 7500 2006 UAL HH Hy time s speed rpm 11000 10000 9000 8000 7000 6000 5000 4000 3000 2000 1000 Fixed Angle Rotor FA12 94 Highconic 7500 2006 Deceleration profiles
11. Overtemperature in the motor deceleration to standstill or 1 dotar Turn instrument off and unplug mains power cord 2 Check and clean ventilation slots if necessary 3 After about 60 min you can restart the instrument Observe the max permissible environmental temperature If the error persists contact the KENDRO service station Rotor stops without Overtemperature in the electronics deceleration to standstill or 1 does not start 2 3 After about 60 min you can restart the instrument Observe the max permissible environmental temperature If the error persists contact the KENDRO service station Turn instrument off and unplug mains power cord Check and clean ventilation slots if necessary 67 Troubleshooting 68 Symptom Rotor stops without deceleration to standstill or does not start Rotor stops without deceleration to standstill or does not start Rotor stops without deceleration to standstill or does not start Rotor stops without deceleration to standstill Instrument cannot be operated Rotor stops without deceleration to standstill Instrument cannot be operated Possible causes and corrective measures Overpressure in the refrigeration system 1 Turn instrument off and pull main plug 1 Check and clean ventilation slots if necessary 2 After about 60 min you can restart the instrument Observe the max permissible environmental temperature If the error persists c
12. and accessories for BlOshield 600 Rotor 7500 2005 Centri Lab Adapter type A 40x 1 5 2 ml micro liter tube 20x 7 mi DIN 12x 7 ml blood sampling 11x15 ml blood sampling 6 x 15 ml conical 12 x 15 ml DIN 4 x 25 ml universal container 5 x 25 ml DIN 2 x 50 ml DIN 2 x 50 ml conical 1 x 50 ml oil test 1 x 100 ml DIN 1 x 150 ml DIN for cyto system Spar seal 1 d diameter 26 max tube length with aerosol tight cap Max tube dimensions Cap Color No of order dx length diameter mm mm 11 0 x 45 SE 160 13 0 80 l 25 0 x 115 6 8 8 ei ani 13 0 x 115 13 0 13 0 x 117 17 0 x 117 17 0 x 120 17 0 x 115 22 5 x 100 34 0 x 120 29 5 x 120 blue 75005325 EREN rer 76003417 AAA A A jl 44 0 x 120 50 0 x 110 Rotors and accessories Table 3 Adapter 4 Max tube Number per Number dimensions adapter per dx length rotor mm Adapter and accessories for Fixed Angle Rotor FA12 94 7500 2006 11x 58 11x 103 13x 115 16 x 96 18 x 124 25 x 112 29 x 118 16 5 x 120 30 x 117 N E i A a i 2 sets incl lubricants Adapter for 16 ml cavity for 10 ml Vacutainer Reducer insert 16 x 100 mm for 7 ml Vacutainer Reducer insert 13 x 100 mm for 5 ml Vacutainer Reducer insert 13 x 75 mm d diameter For 7 ml Vacutainer Adapter 7600 2906 and 7600 3225 required blue 75003102 1x58 AS Ef od 4 _ 193x118 2 16x96 2 18x124
13. changes slowly at first and at an acceler ated pace after a few seconds 4 Release the key as soon as you have reached the desired value and adjust if necessary by repeatedly pressing the key The minute display flashes for a number of seconds then changes to permanent display The run time is now stored You may shift the flashing cursor to set the value as described under Selecting speed Continuous operation To switch the Multifuge to the continuous mode you must press the key bA until the display reads hLd With this setting the centri bid fuge keeps running until stopped manually 46 Extended time mode You have the option of switching to the extended time mode To switch it on or off press the program selec tion key El and simultaneously start the centrifuge As long as you keep the key pressed the selective mode is active Pressing the upward key l l you can switch back and forth between the signal menu beep and the time menu t set After selecting the time menu t set you can switch between the standards time mode 00 0 and the ex tended time mode 00 h by pressing the upward key A The time input in excess of 10 hours is set in one hour increments Operation Selecting the temperature You can select the temperature in the range of 9 C to 40 C To adjust the temperature proceed as follows 1 Press one of the
14. entered Advanced features RCF programming quick run pre temp temperature control during standby Acceleration and deceleration pro 9 acceleration and 9 deceleration profiles files SMARTspin Electronic effective as a function of rotor and speed imbalance detection system Soft touch lid lock Motor assisted lid locking only with refrigeration unit Description of the Multifuge The EASYset user interface Program memory keys Freely programmable Acceleration deceleration profile 1 slowest 9 fastest acceleration deceleration curve Setting speed by rom Adjustable from 300 rpm to 15 000 rpm in 10 rpm increments RCF selection Upon actuation of RCF switch the RCF value can then be entered Time selection Adjustable in minutes from 1 min to 9 h 59 min or extended time mode from 1 min to 99 h hid mode continuous operation Run time display in quick run mode Between 1s and 60 s in seconds steps above in minutes steps Setting temperature Adjustable from 9 C to 40 C in one degree increments End of centrifugation The speed display will read end only with refrigeration unit 10 Description of the Multifuge Lid opening Automatic unlocking via open lid key A unlocking in case of power failure see eee Troubleshooting Quick Run mode Pressing the quick run key activates maximum acceleration up to the maximum permissible speed of the upon key release th
15. 1000 RCF x g 100 1000 10000 100000 speed rpm 100 90 RCF x g Speed RCF diagram Micro liter Rotor 48 x 2 ml 7500 3348 100000 Nmax 15 000 rpm max 9 8 cm min 5 9 cm RCF max Nmax 24 652 1000 1 100 1000 10000 speed rpm 100000 91 92 Index A acceleration and deceleration curves 75 acceleration profiles 7 Acceleration decelaration profile 10 actual values 44 adapter 24 aerosol tight operation 32 aerosol tightness test 36 aluminum rotor 55 approved rotors 38 audible signal can be switched on or off 52 switching on off 52 autoclaving 56 autoclaving cycle permissible maximum 56 B bAL 48 Basic unit function 9 C centrifuge exceptionally noisy 62 Centrifuge transport and installation 13 Centrifuging with a program 50 changing settings during run 48 cleaning 53 closing the lid 37 collet chuck 38 common glass or plastic centrifuge tubes 4 conditions of warranty 57 Conformity 5 contamination necessary measures 54 continuous operation 46 control panel readings 7 corrosion 4 corrosive substances protective tubes for corrosive substances 4 cycle counter 70 93 D dangerous chemicals 3 Deceleration curves 43 decelera
16. 49 0 6184 90 7474 info de kendro spx com Latin America Kendro Laboratory Products International Sales Asheville NC USA Tel 1 828 658 2711 Fax 1 828 645 7581 info kendro spx com North America Kendro Laboratory Products International Sales Asheville NC USA Tel 1 828 658 2711 Fax 1 828 645 3368 info kendro spx com Sorvall and Heraeus products Tel 1 800 522 7746 Internet http www kendro com Sk d Kendro Laboratory Products GmbH en TO Robert Bosch Stra e 1 D 63505 Langenselbold Telephone 49 0 6184 90 6940 In the interest of continuous product development we reserve the right to make changes without express notice 20058041 Multifuge 1S 1S R_uk 04 04 Printed in Germany
17. B A sound pressure level of emission according to DIN EN ISO 11 201 Temperature set range 9 C to 40 C Dimension H x W x D Multifuge 1S 365 mm x 500 mm x 615 mm 14 37 x 19 69 x 24 21 inches Multifuge 1 S R 365 mm x 686 mm x 665 mm 14 37 x 27 0 x 26 18 inches 71 Technical Data Features Weight without rotor Multifuge 1S Multifuge 1 S R Testing standards all devices manufactured and examined in agreement with for 120 V only for 230 V only 72 Specification 92 kg 203 Ib 118 kg 259 Ib IEC 61010 1 1990 amendment 1 1992 amendment 2 1995 IEC 61010 2 020 1993 amendment 1 1996 Pollution degree 2 Overvoltage category Il CAN CSA C22 2 No 1010 1 92 CAN CSA C22 2 No 1010 1 B97 amendment 2 UL 3101 1 EN 292 EN 61 010 1 EN 61 010 2 020 EN 61326 EN 55011 B Technical Data Electrical connections fuses Order no Voltage Frequency Nominal Power Fuse protection of Fuse current consumption instrument protection of safety fuse building thermal excess current release Multifuge 1 S 7500 4311 230 V 50 60 Hz e 73 Notes 74 Appendix Acceleration and deceleration profiles On the following pages you find acceleration and deceleration profiles for each rotor type respectively 75 76 speed rpm 5000 4000 3000 2000 1000 Acceleration profiles Swinging Bucket Rotor TTH 400 75002000
18. Heraeus Multifuge 1 S 1 S R Instruction Manual How to use this manual Use this manual to get acquainted with your centri fuge and its accessories This manual helps you to avoid inappropriate han dling Make sure to keep it close to the centrifuge A manual that is not kept handy cannot provide protection against improper handling and thus against damage to persons and objects The manual comprises chapters on e Safety regulations e Instrument description e Transportation and hook up e Rotor program and accessories e Use of the centrifuge e Maintenance and care e Troubleshooting e Technical data e Index Overleaf you will find a graphic representation of the control panel with a survey of the most important functions Please fold out acceleration deceleration only for instruments with refrigeration unit profiles profiles temperature speed RCF run time Quick run program selection l a E open lid stop po A MULTIFUGE 1 s a Herae s start rpm RCF Bucket selection switch key display set keys pre temperature regulation set keys Before switching on the centrifuge please read this manual The mains switch is located on the right hand side panel Pressedup ON Pressed down OFF 0 Control panel of the Multifuge 1S 1 S R Display panels Program selection key 1 4 store or recall programs acceleration profiles 1 slowest
19. L P res PR 0000 A ptre an e Both of the levers must click in order to achieve aerosol tight bio containment Levers not being flapped down TAG may cause damage to the caps during centrifugation Both of the levers must click in order to Ee achieve aerosol tight bio containment 33 Rotors and accessories Closing the round bucket 75002002 aerosol tightly After greasing the seal turn the lid until it sits lightly on the bucket To achieve uniform seal turn the lid tighter by 1 grip area approx 10 Use the setting marks on the bucket as a guide Grip area Bucket mark 34 Closing Fixed Angle Rotor aerosol tightly The hexagon wrench should be used as a support tool to fasten and loosen the lid of the fixed angle rotor in order to achieve secure closing insert the hexagon wrench through the hole in the screw cap Please pay attention to the maximum permissible filling volume during cen trifugation of dangerous samples Rotors and accessories Micro Falcon Micro Liter Rotor 48 x 2 0 ml 75003348 Fixed Angle Rotor FA12 94 75002006 Vessel type maximum filling volume Micro 1 5 ml Micro 2 0 ml 1 0 ml 1 5 ml others 2 3 nominal volume micro centrifuge tube tube type Falcon Falcon 50 ml Falcon 15 ml 49 ml 14 ml 35 Rotors and accessories Checking of aerosol tight bio containment The checking of the rotor typ
20. ab Adapter type A 40x 1 5 2 ml micro liter tube 20x 7 mi DIN 12 x 15 ml DIN 4 x 25 ml universal container 5 x 25 ml DIN 2 x 50 ml DIN 2 x 50 ml conical 1 x 50 ml oil test 1 x 100 ml DIN 1 x 150 ml DIN for cyto system Aerosol tight caps Spar seal d diameter 24 Max tube length with aerosol tight cap Max tube dimensions d x length mm Cap diameter mm 13 0 x 117 17 0 x 117 17 0 x 120 11 0 x 45 13 0 x 115 17 0 x 115 22 5 x 100 25 0 x 115 34 0 x 120 29 5 x 120 4 0 x 120 0 0 x 110 11 0x 45 130x115 17 0x115 22 5 x 100 250x115 340x120 29 5 x 120 AM E 160 blue aa Order no 7500 2022 7500 2021 7500 2020 7500 5335 7500 5321 7500 5322 7500 5391 7500 5323 7500 5324 7500 5386 7500 5339 7500 5325 7500 5326 7600 3417 2 pieces incl seals and lubricants 4 pieces incl lubricants 7500 2003 7500 2008 Rotors and accessories Table 3 Adapter 2 Max tube length with aerosol tight cap a Max tube Adapter and accessories for dimensions Cap round buckets 7500 2002 d x length diameter mm mm Color No of order 45 0x134 590 4 bue 75007575 62 0x135 4 nature 75007622 80 0x135 4 nature 75007583 Spar seal 4 pieces incl lubricants 1 d diameter 25 Rotors and accessories Table 3 Adapter 3 Adapter
21. alanced run occur although the weight is correct this could be due to wear of the slide coating In this case the rotor function remains intact through normal lubrication at regular intervals apply a light coating of lubricant to the rotor body trunnion pins and to the corresponding mating surfaces of the buckets e Lubricant 7000 6692 is supplied with the centrifuge 29 Rotors and accessories BIOshield 600 Rotor 7500 2005 Do not run the rotor without the rotor E Always maintain the rotor in the recom cover installed mended manner The rotor and accessories must be cleaned Store the BIOshield Rotor with the cover removed Fe inspected Mei do not i when after cleaning This will enable the rotor to dry SNOWING SIgRS OOO CraACKINg thoroughly 30 Rotors and accessories Micro plate carrier MP 3300 7500 2010 Remove the appropriate plate holder from E the carrier body for loading and unloading of micro plates in the Micro plate carrier Before loading ensure that the rubber bottom is placed in the cut outs of the bottom of the plate holder The maximum loading height amounts to 55 mm ce Make sure the rotor is balanced The operation is permissible only with the associated carriers Label 75002010 Some commercially available micro plates are not rated for the maximum achievable RCF of the Microplate car rier Therefore please pay attention to the specifications of t
22. anufacturer you have to ensure that the foreseen pro cedure does not cause any damages to the instrument by consulting the manufacturer Cleaning Pull mains plug before cleaning the instrument e Clean the case the rotor chamber the rotor and the accessories regulariy and in case of need This is indicated both for reasons of hygiene and to prevent corrosion due to contamination sticking to the instru ment and its accessories Clean them with mild agents of pH values ranging from 6 to 8 For other cleaning agents please consult KENDRO Services Immediately after cleaning dry the aluminum parts or put them into a warm air dryer at a temperature not exceeding 50 C 53 Maintenance and care During cleaning liquids and espe cially organic solvents should not AE come into contact with the drive shaft and the ball bearing Organic solvents may decompose the lubricant of the motor bearing The drive shaft may seize Instruments with refrigeration unit If a strong ice sheet is present in the internal E chamber be sure to remove all condensa tion after defrosting Please clean the venting slots regularly please disconnect the centrifuge from the mains supply Please pull mains plug Before cleaning the venting slots 54 Disinfection If a centrifuge tube containing infectious material leaks during a run you have to disinfect the centrifuge im medi
23. art key in the control panel The centri fuge accelerates to the selected value Simultaneously the time display starts counting down from the selected set time giving the remaining run time in minutes dur ing continuous operation the time display goes for ward If a value exceeding the maximum permissible speed or RCF of the respective rotor was entered this is indi cated after the start of the centrifuge by the alternately flashing messages rotor and the maximum permissi ble value for the inserted rotor Within 15 seconds you may adopt this value by again pressing the start key the centrifugation is then con tinued Otherwise the centrifuge stops and you must enter a permissible value You cannot open the lid during the run 48 Imbalance display If rotor imbalance is detected shortly after the rotor reaches 300 rpm the message bAL will appear in the speed display The run is terminated BAL O and you may restart the centrifuge after correct ing the imbalance check loading Changing the settings during the run You can change all settings during a run By pressing any one of the set keys in the control panel once you can switch from the actual to the set point mode The to be adjusted setting flashes and can then be altered Once the data input is finished and the display has changed to the actual value display mode the new settings become operative Operation Stopping t
24. ately A Infectious material could enter the centrifuge if spills or tube breakage occur Danger of infection may occur upon contact Take appropriate protective measures for personnel Pay attention to the permissible filling volumes and loading limits for the tubes In case of contamination the operator has to ensure that no other persons are at risk Contaminated parts will have to be decontaminated immediately If required further protective meas ures have to be initiated Maintenance and care Rotor and rotor chamber must be treated with a neu tral universal disinfectant Best suited for this purpose are disinfectant sprays ensuring that all rotor and ac cessory surfaces are covered evenly e Please use 70 ethanol for disinfection Please pay attention to the safety measures and handling requirements when applying these substances For other disinfectants please consult KENDRO Services e You may disinfect the rotor and the accessories as described in the following section Be sure to follow the pertinent safety procedures for handling infec tious material 1 Pull mains plug 2 Unscrew the rotor chuck 3 Grab the rotor with both hands and pull it perpen dicularly off the drive shaft Remove the centrifuge tubes and adapters and disinfect them or dispose of them as necessary Treat the rotor and the rotor lid according to the instructions given for t
25. be silenced upon pressing any key You have the option of signaling the end of a run acoustically To activate or deactivate this option press the program key and simultaneously switch on the centrifuge As long as you keep this key pressed the selection mode is active The speed panel shows beep and the time panel shows on or off By actuating the upward key l in the time panel the signal function can be switched on or off e When the message rotor flashes pressing the start key once is sufficient to turn off the warning signal and to accelerate the rotor to the maximum speed displayed by the in strument 52 Turning off the centrifuge The centrifuge is turned off by switching the main switch into the O position eS The main power switch should be turned off after a complete centrifugation run Without motor deceleration it takes much longer until the rotor comes to a halt The centrifuge lid can only be opened automatically if the centrifuge is turned on Maintenance and care Maintenance and care Maintenance to be performed by the customer For the protection of persons environment and mate rial you are obliged to clean the centrifuge regularly and to disinfect it if necessary Unsuitable cleaning agents or disin fection procedures may damage the centrifuge and its accessories If you intend to use cleaning agents or disinfection procedures not rec ommended by the m
26. broken tube damage to the rotor or motor malfunction is responsible for the noise If you cannot locate and solve the problem yourself contact a KENDRO service station Message bAI appears Rotor stops with Imbalance switch actuated in display deceleration Open the instrument by pressing open lid key EI Check whether the rotor is properly loaded Check whether a broken tube or damage to the rotor was responsible for imbalance switch actuation Check that the trunnions of the swinging bucket rotor have been properly lubricated If you cannot locate and solve the problem yourself contact the KENDRO service station 62 Troubleshooting Message rotor appears in display Display OPEN appears although lid is closed Message Lid appears flashing in the display Message Lift Lid appears in the display Symptom Rotor decelerates with delayed deceleration Will not start Drive stops Rotor stops without deceleration to standstill Lid does not open automatically Possible causes and corrective measures Set speed exceeds permissible maximum speed for the rotor The same holds for RCF setting A For about 15 sec the display shows alternately rotor and the maximum permissible speed or RCF for the installed after the rotor identification Within this period it is possible to accept this value by again pressing the start key The centrifugation will then be con
27. e and bucket was done according to the dynamic microbiological test proce dure with regard to EN 61010 2 020 appendix AA The aerosol tight bio containment of the rotor mainly depends on proper handling Check the aerosol tight bio containment of your rotor whenever necessary Ez It is very important that all the seals and seal surfaces are carefully inspected for wear and damages like cracks scratches and embrittlements For a quick test one can check the aerosol tight buck ets and fixed angle rotors according to the following procedure e Slightly grease all seals e Fill the bucket or rotor with approx 50 ml carbon dioxide mineral water e Close the bucket or rotor according to the respec tive handling instructions 36 Shaking the bucket releases the carbon dioxide of the water and an excessive pressure is built up Leaks are recognized by humidity release and audible disinflation of gas mix Finally buckets respectively rotor lid and lid seal have to be dried Operation Operation Switching on the centrifuge Locate the main power switch on the right hand side of the front panel and set it to the ON I position For a couple of seconds the following reading appears in the control panel 00 CI CS EE 2 EEE CER E E Va velva Tam cm MULTIFUGE 1 s a The display shows that the instrument is going through an internal check of its software See table on page 70 After this chec
28. e centrifuge stops with maximum deceleration power Diagnostic messages Alternating display Rotor maximum speed or RCF speed selected exceeds max speed of the rotor Lid has not been lifted off the lock during opening display lift lid manual lifting of lid is required General instrument malfunction error messages with ERROR codes see Troubleshooting 11 Description of the Multifuge Notes 12 Before use Before use Centrifuge transport and installation After opening the box remove the protective materials When transporting the centrifuge consider its weight see Technical Data always grab it on both sides ensuring that enough helpers are around Do not lift on the front panel A The centrifuge can be damaged by jolting during the transport Transport the centrifuge only in the upright position using proper con tainment and secure it properly Handle the centrifuge carefully Proper location The centrifuge may only be used indoors Its location must meet the following criteria A safety zone of at least 30 cm 12 inches around the centrifuge must be maintained Hazardous ma terials must not be stored beside the centrifuge dur ing its use The laboratory bench or centrifuge trolley must be stable and resonance free A good support is pro vided by a laboratory bench or a centrifuge cart with lockable ca
29. he centrifuge Stopping with preset run time Normally the run time has been selected and all you have to do is to wait until the centrifuge terminates the run automatically at the end of the set time As soon as the speed reaches zero the display reads end You can now open the centrifuge by pressing the open lid key and remove your samples If the lid has not been fully lifted out of the lid lock the message lift lid appears you must manually lift the lid You can manually stop the centrifuge at any time by pressing the stop key IMI At this point the remaining run time is displayed Stopping with continuous operation If you have chosen continuous operation you must stop the centrifuge manually Press the stop key IE in the control panel The centrifuge starts deceleration with the preset deceleration profile The display reads end and you can open the lid by pressing the open lid key IA and remove your sam ples Temperature control during standby Temperature control becomes active once the rotor has been identified This is the case after a centrifuga tion run exceeding 300 rpm At standby the display reads end If the rotor has not been identified lid has been closed and the start key LI has not yet been pressed speed panel shows 0 with flashing point the instru ment regulates the temperature so that the samples cannot freeze in any one of the usable rot
30. he disinfectant soaking in liquid or spraying You must strictly observe the specified action times Turn the rotor head down and drain off the disinfec tant Afterwards thoroughly rinse rotor and lid with water Dispose of the disinfectant according to valid regu lations Aluminum rotors have to be treated with anticorro sive protective oil subsequently Disinfection with bleaching lye These agents contain highly aggres sive hypochlorites and must not be used with aluminum rotors 55 Maintenance and care Decontamination For general radioactive decontamination use a solu tion of equal parts of 70 ethanol 10 SDS and wa ter Follow this with ethanol rinses then de ionized water rinses and dry with a soft absorbent cloth Dis pose of all washing solutions in appropriate radioactive waste containers Autoclaving ES Check whether autoclaving is permitted You may autoclave the rotor and the adapters at 121 C Maximum permissible autoclaving cycle 20 min at 121 C The rotor must be cleaned and rinsed with distilled water before being autoclaved Remove the rotor lid the centrifuge tubes and the adapters Place plastic rotors on an even surface to avoid deformation 56 Chemical additives to the steam are not permitted Never exceed the maximum permis sible values for autoclaving tempera ture and autoclaving time Should the rotor show signs of wear you must
31. he plate manu facturer 31 Rotors and accessories Aerosol tight operation Aerosol tight rotors and tubes are only to be opened in an approved safety work bench when centrifuging danger ous samples It is necessary to pay attention to the maximum permissible filling quantities A Correct operation when filling the sample tubes and closing the rotor cover are prerequisites for aerosol bio containment A Before use the seals in the rotors and rotor covers as well as the aerosol tight caps have to be checked for abrasion or damage and have to be slightly greased Replace damaged O rings and seals 32 Use the special lubricant 7600 3500 only to grease the seals e Spare parts are delivered with the rotor or may be or dered separately Replace damaged or clouded caps and lids of rotors and tubes immediately The tubes are only to be filled in such a way that the sample does not reach the rim of the tube during cen trifuging Rotors and accessories Closing the rectangular bucket 75002001 Closing the Micro test plates rotor MP3300 aerosol tightly 75002010 After putting on the cap both levers are pressed down osol tightly until they noticeably click in Please flap both lock levers upward The cap can now SORVALL SORVALL be easily put on the bucket The bucket is locked Heraeus Heraeus through flapping down the levers 75002001 L 75002001
32. ifferences of 120V instruments are shown in parentheses Rotor designation BlOshield 600 Rotor Micro plate carrier 4 x 150 ml MP 3300 Order no 75002005 75002010 Maximum permissible load g 4 x 540 2 x 600 Maximum speed nmax rpm 6000 4400 Maximum RCF value at nmax 6842 3355 Radus max temi Acceleration deceleration time s relative to room temperature 23 C Autoclavable 121 C Tested and approved by CAMR Porton Down UK 22 Rotors and accessories Table 2 Rotors for Multifuge 1 S R Differences of 120V instruments are shown in parentheses Rotor designation Fixed Angle Rotor FA12 94 Highconic Micro Liter Rotor 6 x 94 ml 12 x 16 ml 48 x 2 0 ml Order no 75002006 75003348 Maximum permissible load g 6x 140 12x30 Maximum speed nmax rpm 10 350 15 000 Maximum RCF value at nmax 15 090 24 652 outer ring 21 885 inner ring Radius max min cm 12 5 6 1 9 8 5 9 i Acceleration deceleration time s 45 45 55 50 30 30 40 30 Min temperature at nma C 0 4 4 6 relative to room temperature 23 C Speed at 4 C rpm 10 350 15 000 14 500 Y Y Aerosol tight es Autoclavable 121 C Tested and approved by CAMR Porton Down UK 23 Rotors and accessories Adapter Table 3 Adapter 1 Adapter and accessories for rectangular buckets 7500 2001 Centri Lab Adapter type A plus 14x 7 ml blood sampling 12 x 15 ml blood sampling 8 x 15 ml conical Centri L
33. imum speed for the rotor identification exceeded Switch the instrument off and on again If the problem persists a contact KENDRO service station Bucket code for this rotor is not defined Check whether the bucket is permitted for the inserted rotor rotor program page 16 Troubleshooting Symptom Possible causes and corrective measures Instrument stops with No rotor present or rotor identification impossible deceleration to standstill after short starting up A Check if a certified rotor is inserted B Please pay attention to the legibility of the inscription of the swinging bucket rotor cross when it is installed rotor identification must show to the chamber bottom For the swinging bucket rotor the set bucket type must be permitted for the operation in the respective rotor Please note the hints of chapter Bucket selection of swinging bucket rotors page 43 and compare the permitted rotors and rotor buckets in table 1 on page 16 Following a brief power failure the rotor could not be identified Switch the instrument off and on again using the mains switch Instrument does not start or Rotor cannot be identified decelerates to standstill A Check if a certified rotor is inserted B Check if the rotor is completely fastened onto the motor shaft For this release the rotor fastening until you can lift and depress it easily Now tighten the rotor fastening once again 65 Troubleshooting
34. k the display shows the actual value mode The remaining run time and speed should both read 0 The display of the acceleration deceleration curve depends on the last set value The following figure gives an example of possible read ings A detailed description of possible settings is given below in this chapter E u a BEWE WEBE e VA u Vao Actuating the lid Opening the lid Press the open lid keyli If the message Lift lid appears slightly lift the lid Emergency release in case of malfunction or power failure see chapter Troubleshooting Closing the lid The centrifuge lid is locked by slightly pressing down the front part of the lid Locking is motor driven E Do not slam the lid 37 Operation Installing the rotor Improper or improperly combined accessories may cause severe dam age to the centrifuge The rotors approved for the Multifuge are detailed in the chapter Rotors and accessories Use only rotors listed for this instrument To install the rotor you will need the wrench see chap ter Parts supplied page 8 Proceed as follows 1 Open the lid and make sure that the rotor chamber is clean Clean any dust foreign material or sample residues out of the chamber before use 2 Check if the collet chuck is loose collet chuck moves freely on the spindle If not loosen the rotor seat using the supplied socket wrench 3 Place the rotor on t
35. ked E 31 Overtemperature in motor E 32 Overtemperature in electronic E 33 Excessive pressure in the refrigeration unit E 34 Overvoltage E 35 Overcurrent Lift Lid Smoothly lift the lid rotor set speed higher than permissible speed of the rotor bAL imbalance Contents Contents For your safety j 3 Proper USE keyn dka ki oda a ani eli 3 lmproper USE eh n a yaki Meki anda sin la ata yay V n 3 Centrifuging hazardous materials 3 Handling the cenitrifuge U Eu 4 Conformity to current standards 5 Safety instructions in this manual 5 The Multifuge 1 S 1 S R 7 Description i e Pal aaa n assoc dias 7 Safety SYSTEMS iin caw neni 8 Parts supplied nak eae cake ceed ta aa 8 Function and features i 9 Before User 13 Centrifuge transport and installation 13 Proper location nenea en anna 13 Main connection i 14 Rotors and accessories 15 Rotors for the Multifuge S nl keneke cauta i au la 16 Rotors for the Multifuge 1 S R 20 Handling the rotor eee 29 Aerosol tight operation i 32 Checking of aerosol tight bio containment 36 Operation ii 37 Switching on the cenitrifuge
36. lue 16 17 21 maximum sample density 3 microtest plates 31 minimum distance from wall 13 O opening the lid 37 operation short time 51 organic solvents not allowed for cleaning 54 95 P pathogenic microorganisms protection against 4 Plastic sample tubes Pretemp function 47 pretemperature regulations 47 problems handling 61 program display symbols 50 Program memory keys 10 program storing 50 protective tubes for corrosive substances 4 Q quick run function 51 Quick run mode 11 R radius of centrifugation for calculation of RCF value 45 RCF maximum 48 96 RCF speed display 43 RCF display and rotor identification 44 RCF selection 10 RCF setpoint 44 RCF value 7 45 relative centrifugal force 45 RCF value 20 22 23 RCF value 18 19 readings of control panel during run 7 Regulations for prevention of accidents 5 relative centrifugal force 45 remain dark 61 rotor cover 38 rotor identification 8 rotor identification 44 and RCF display 44 Rotor program for the Multifuge 16 rotors approved 38 run profile 7 run time range 45 selection 45 S safety instructions 3 Safety instructions 5 safety measures 3 safety standards 5 safety zone 3 13 sample density maximum 3 sample tubes are approved 40 selecting run time 45 Selecting speed 44 selecting the temperature 47 Selecti
37. me s 40 50 60 45 35 30 40 35 Heating of samples 15 0 C 12000 1h C atrpm run time 9 0 C 10150 1h 15 0 C 15000 15 min relative to room temperature 23 C 23 0 C 15000 1h Aerosol tight Autoclavable Tested and approved by CAMR Porton Down UK 19 Rotors and accessories Rotors for the Multifuge 1 S R Table 2 Rotors for Multifuge 1 S R Differences of 120V instruments are shown in parentheses Rotor designation Swinging Bucket Rotor TTH 400 75002000 With bucket Rectangular bucket Round bucket 150 ml 400 ml Order no 75002001 75002002 Maximum permissible load g 4 x 540 4 x 570 Maximum speed nmax rpm 4700 4600 4700 relative to room temperature 23 C Tested and approved by CAMR Porton Down UK 20 Rotors and accessories Table 2 Rotors for Multifuge 1 S R Differences of 120V instruments are shown in parentheses Rotor designation Swinging Bucket Rotor TTH 400 75002000 With bucket Multiple carriers Multiple carriers 14 x 15 ml 5x 50 ml 2x15 ml Order no 75002027 75002028 Maximum permissible load g 4 x 420 4 x 430 Maximum speed Nmax rpm 4700 4500 4700 4500 Maximum RCF value at nmax 4642 4256 4593 4210 Acceleration deceleration time s 40 35 65 40 40 35 65 40 Heating of samples at nmax C relative to room temperature 23 C run time 1 hour 21 Rotors and accessories Table 2 Rotors for Multifuge 1 S R D
38. n MI Operation Quick Run For short time operation the Multifuge is equipped with a quick run function Short time centrifugation is started by pressing the quick run key continuously it stops as soon as the key is released In this mode the centrifuge accelerates with full power up to the maximum speed The set speed or RCF is ignored in this case Depending on the rotor the centrifuge accelerates to the maximum speed Check carefully whether you have to maintain a specific speed for your application e During acceleration the time is counted forward in sec onds The display remains until the centrifuge lid is opened Removing the rotor 1 Open the centrifuge lid 2 Remove the rotor cover on applicable rotors 3 Unscrew the clamping sleeve counterclockwise using the socket wrench supplied with the instru ment until no resistance exists 4 Grab the rotor with both hands and carefully pull it perpendicularly off the drive shaft Make sure not to tilt it e Grab rotor with both hands and pull upwards perpendicularly When using an aerosol tight bio containment cover you may remove the respective rotor from the drive shaft without opening the cover You may then open the rotor e g in a safety work bench and decontami nate it 51 Operation Audible alarm Accompanying all error messages a warning signal is given out which can
39. n mechanical or electrical components of the centrifuge may only be carried out by individuals authorized by Kendro Laboratory Products For your safety Conformity to current standards Safety instructions in this manual Heraeus centrifuges are manufactured and tested This symbol denotes potential hazards to according to the following standards and regulations A persons For all voltages This symbol denotes potential damage to e IEC 61010 the centrifuge or parts in its immediate surroundings For 120 V only GP General comments are marked with this E symbol C US In addition you are asked to adhere to the pertinent regulations in Germany For 230 V only e Regulations for prevention of accidents BGV A2 e Regulations for prevention of accidents VBG 5 e Regulations for prevention of accidents VBG 7z with cooled devices additionally Please get acquainted with the details of the test stan Regd Le pie valorii o abs BOVEY dards from the technical data For your safety Notes Description of the Multifuge The Multifuge 1 S 1 S R The figure below shows a Multifuge 1 S R with an opened lid and a swinging bucket rotor installed OOo Description The Multifuge 1S R is a general purpose tabletop centrifuge for biotechnological and pharmaceutical research that moves high capacity centrifugation onto the fast track It spins more tubes at higher RCFs more rapidly than competitive in
40. ng the temperature 47 service 57 service contracts 57 setpoint values 44 setting speed 44 settings change during run 48 Short time centrifugation 51 short time operation 51 signal 52 software check internal 37 software version determination 70 speed maximum 48 speed RCF display 43 speed of centrifugation for calculation of RCF value 45 Speed selection 10 speed setting 44 Starting the centrifuge 48 stopping manually 49 with continuous operation 49 Stopping with preset run time 49 substructure 13 Switching from speed to RCF display and vice versa 43 symbols for hazards and dangers 5 T Technical Data 71 test aerosol tightness 36 The printed documents consist of the delivery notes and this Manual 8 time counted forward in quick run mode 51 tools for installing the rotor 38 toxins protection against 4 troubleshooting 59 t set 97 time mode 46 tube breakage with infectious material 54 Turning the centrifuge off 52 V volume 98 maximum filling 35 W warning signal 52 warranty conditions 57 wear 4 For Ordering or Technical Information Asia Pacific Kendro Laboratory Products International Sales Asheville NC USA Tel 1 828 658 2711 Fax 1 828 645 7581 info kendro spx com Europe Middle East Africa Kendro Laboratory Products International Sales Langenselbold Germany Tel 49 0 6184 90 6940 Fax
41. nnel e their design is improperly changed e the safety instructions are not followed Therefore personnel involved with operation and maintenance of the centrifuge must read and follow the safety instructions In addition the pertinent regulations for prevention of accidents must be strictly followed E A This manual is an integral part of the centri fuge assembly and must be kept close at hand at all times When damages to the power cord or at casing are noticed the centrifuge must to be set out of operation Proper use The centrifuge is designed to separate liquid suspended materials having different densities and particle size respectively maximum sample density is 1 2 g cm ml at maximum speed Improper use During a run a safety zone of 30 cm around the centri fuge must be maintained where neither persons nor hazardous materials may be present The centrifuge may cause harm to its user or other persons or may damage goods if safety measures are not followed Centrifuging hazardous materials e The centrifuge is neither made inert nor is it explo sion proof Therefore never use the centrifuge in an explosion prone environment e Do not centrifuge explosive or flammable sub stances The same holds for substances prone to react violently with each other For your safety Do not centrifuge toxic or radioactive substances or pathogenic microorganisms without suitable safety system
42. ontact the KENDRO service station Overvoltage in the intermediate circuit Switch the instrument off and on again If the error persists contact the KENDRO service station Overcurrent in the intermediate circuit Switch the instrument off and on again If the error persists contact the KENDRO service station Overcurrent or error in current measurement Switch the instrument off and on again If the error persists contact the KENDRO service station Speed control measurement exceeds permissible rotor speed Switch the instrument off and on again If the error persists contact the KENDRO service station Troubleshooting Symptom Possible causes and corrective measures Rotor stops without Acceleration of rotor too slow deceleration to standstill Open the lid and check the load Instrument cannot be Significant imbalance caused by missing bucket operated Tubes or adapter do not fit rotor is rubbing the chamber or the cover Micro plates carriers on the motor cover If the error persists contact the KENDRO service station 69 Troubleshooting Contacting Kendro Service Should you require help from our service station please indicate the catalog and serial number of your instrument You will find the pertinent information at the specifications near the socket for the main plug Moreover it is helpful for our service representative to know your software version You can determine the software
43. op of the collet chuck so that the rotor is located precisely above the center 38 4 The rotor must glide freely down the collet chuck until it hits the lower stop 5 If you have positioned the rotor correctly you can tighten the collet chuck easily using the supplied hex wrench 6 Place the rotor cover on applicable rotors and tighten it securely E Regularly check the proper positioning of the rotor and re tighten the collet chuck as needed Please take care of the legibility of the inscription of the installed swinging bucket rotor cross Rotor identification must point to the chamber bottom For the swinging bucket rotor the set bucket type must be permitted for the operation in the respective rotor Please note the requirements of chapter Bucket selec tion of swinging bucket rotors Operation Loading the rotor Maximum loading Overloading can result in destruction and severe damage to the centrifuge The Multifuge can reach high rotational speeds exert ing enormous centrifugal force The rotors are de signed to warrant sufficient residual strength even at the highest permissible speed However this safety system presupposes that the maximum permissible load of the rotor is not exceeded e Please get acquainted with the data about the maximum permissible loads and maximum speeds in chapter Ro tor and accessories If you wish to centrifuge samples that together with the adapter
44. ors If you find the systematic deviation in temperature of up to 4K unacceptable you have to start the centrifuge for a short period of time until the rotor is identified 49 Operation Working with programs The 4 program selection keys offer the option of storing and recalling the individual centrifugation processes ajo GIS 4400 o MULTIFUGE 1 s a Heraeus Program display By actuating one of the program selection keys the specific set point values stored are displayed Entering changing a program All program places have been factory set at the same values To change these values proceed as follows Enter the desired parameters Only the selection panel affected by the changes is flashing After entering all set point values press the desired program selection key for approx 3 Seconds gt The entering is confirmed by the signal the new program is stored Repeat the process to set values to additional program places 50 Centrifuging with a program After closing the centrifuge lid call the desired program memory number using the program selection key and press the start key Hz If the rotor is started with a program the speed or RCF set point value which exceeds the permissible value of the inserted rotor or the RCF set point which is below the rotor specific minimum the program selection keys LED of the program chosen beforehand will be switched off after the rotor identificatio
45. re about the RCF value The relative centrifugal force RCF is given in multi ples of the earth gravity g It is a dimensionless number that allows comparing the efficiency of separation or sedimentation of diverse instruments since it is inde pendent of the instrument used The only values en tered in the equation are radius and speed of centrifu gation 2 n y wa r radius of centrifugation in cm n speed in rpm RCF 1 bs The maximum RCF value refers to the maximum ra dius of the tube bore Please note that this value decreases depending on the tubes and adapters used You may take this into account when calculating the RCF value for your application Selecting run time There are two time modes standard and extended In the standard time mode you can select a run time between 1 min and 9 h 59 min or continuous operation hLd In the extended mode you can select a run time be tween 1 min and 99 h from 10 h in one hour steps or continuous operation hLd Run time selection To set a time proceed as follows 1 Press one of the set keys lA for an increase or MI for a decrease in the run time section of the control panel once to switch from the actual to the set point mode 2 By briefly pressing the set key you can now raise or lower the run 18 9 D time in 1 minute incre ments 45 Operation 3 If you keep the selected key pressed the dis play
46. rifugation heat is generated by air friction between the rapidly spinning rotor and the air inside the rotor chamber The continuous air flow in the air cooled centrifuge is restricting the temperature rise of the samples The equilibrium temperature rise de pends on the ambient temperature the type of rotor the speed the duration and the number of runs they may be hot after long runs at high speed Use caution when touching rotors as 15 Rotors and accessories Rotors for the Multifuge 1 S Table 1 Rotors for Multifuge 1 S Differences of 120V instruments are shown in parentheses Rotor designation Swinging Bucket Rotor TTH 400 75002000 With bucket Rectangular bucket Round bucket 150 ml 400 ml Order no 75002001 75002002 Maximum permissible load g 4 x 540 4 x 570 Maximum speed nmax rpm 4700 4500 4700 Maximum RCF value at nmax 4618 4234 4618 Radus max om Acceleration deceleration time s 40 35 50 40 40 35 55 40 Heating of samples at nmax C relative to room temperature 23 C 12 0 14 0 13 0 run time 1 hour Aerosol tight Yes with cap 75002003 Yes with cap 75002004 Autoclavable 121 C 121 C Tested and approved by CAMR Porton Down UK 16 Rotors and accessories Table 1 Rotors for Multifuge 1 S Differences of 120V instruments are shown in parentheses Rotor designation Swinging Bucket Rotor TTH 400 75002000 With bucket Multiple carriers Multiple carrie
47. rotor is at a complete stop mergenecy ua release observe through window in the cover In case of a power failure the lid cannot be opened normally using the electrical lid unlocking mechanism en du i To permit unloading in this case the centrifuge is During a power outage it is impossible equipped with an emergency override release How A to lock the lid once the emergency lid ever you may only use this system in case of emer release has been used Never stop the gency rotor using your hands or using tools ing them may cause severe injuries 3 Facing the centrifuge under the left hand side there is a plastic plug that can be removed using a screwdriver After removal use your fingers or a pair of pliers to pull the attached cord and activate the manual door override The lid will open and you can remove your samples Rotors can spin at high speed Touch 2 Unplug the main power cord Always wait for several minutes until the rotor has come to a complete stop In case of a power outage the brake does not function and deceleration takes much longer than normal m a Emergency release 59 Troubleshooting 4 Afterwards push the cord back into the instrument and reinsert the plastic plug Once the power is restored you can connect the in strument to the main supply and turn it on Following the self test of the centrifuge the lid may be closed and locked with the motor 60
48. rs 14x 15 ml 5x 50 ml 2x15ml Order no 75002027 75002028 Maximum permissible load g 4 x 420 4 x 430 Maximum speed Nmax rpm 4700 4500 4700 4500 Maximum RCF value at nmax 4642 4256 4593 4210 Acceleration deceleration time s 40 35 65 40 40 35 65 40 Heating of samples at nmax C relative to room temperature 23 C run time 1 hour 17 Rotors and accessories Table 1 Rotors for Multifuge 1 S Rotor designation Order no Maximum permissible load g Maximum speed nmax rpm Maximum RCF value at nmax Radius max cm Acceleration deceleration time s Heating of samples at nmax C relative to room temperature 23 C Run time 1 hour Aerosol tight Autoclavable Differences of 120V instruments are shown in parentheses BlOshield 600 Rotor 4 x 150 ml 75002005 es 121 C Tested and approved by CAMR Porton Down UK 18 Micro plate carrier MP 3300 75002010 121 C Rotors and accessories Table 1 Rotors for Multifuge 1 S Differences of 120V instruments are shown in parentheses Rotor designation Fixed Angle Rotor FA12 94 Highconic Micro Liter Rotor 6 x 94 ml 12 x 16 ml 48 x2 ml Order no 75002006 75003348 Maximum permissible load g 6x 140 12x30 Maximum speed nmax rpm 10 150 15 000 Maximum RCF value at nmax 14 513 24 652 outer ring 21 885 inner ring Radius max min cm 12 5 6 1 9 8 5 9 i Acceleration deceleration ti
49. s If microbiological samples of risk group Il according to Laboratory Bio safety Manual of WHO are be ing centrifuged aerosol tight bio seals have to be used For materials in a higher risk group more than one precaution is required Should toxins or pathogenic substances enter the centrifuge or its parts you must perform appropriate procedures for disinfection see Maintenance and care Disinfection Strongly corrosive substances that may cause damage to materials and reduce the mechanical strength of the rotor may be centrifuged only inside protective tubes Handling the centrifuge Use only original accessories for the centrifuge The only exceptions are common glass or plastic centri fuge tubes if they are approved for the rotor speed and RCF values Never use the centrifuge unless the rotor is properly installed You may use the centrifuge only with a properly loaded rotor You must not overload the rotor Strictly follow the rules and regulations for cleaning and disinfection If the rotor or the rotor lid shows signs of corrosion or wear you must stop using it Never open the lid manually if the rotor is still turn ing You may use the emergency lid release only in case of emergency e g during an interruption of power supply see chapter Troubleshooting Never use the centrifuge with an opened lid Never use the centrifuge if the front panel has been partially or totally removed Changes i
50. s exceed the maximum permissible load you must either reduce the sample volume or calculate the permissible speed npem according to the following for mula i maximum permissible load max n DENN actual load Npem permissible speed Nmax maximum speed 39 Operation Filling the centrifuge tubes Check carefully whether your tubes are approved for the respective RCF value Follow tube manufacturer s recommendation For common borosilicate glass tubes the maximum permissible rcf is limited to 4000 xg The tube manufacturers normally limit the respective maximum allowed RCF value to the fixed angle rotor Please note that for the same RCF value the stress for the tubes in a swinging bucket rotor is higher Because of the higher difference of the radii fmax fmin the pressure of liquid column to the tube bottom is appreciably higher and strongly depended on filling Plastic tubes and bottles especially for the highest load speed temperature have a limited life time and must be replaced as recommended by the manufac turer 40 Maximum permissible load difference cer The smaller the imbalance of the centrifuge the better the separation effect because as imbalance is minimized so is the resulting vibration which could affect separation quality Therefore it is important that the tubes are properly balanced In case of exceeding the rotor specific imbalance val ues the elec
51. sters To ensure sufficient air circulation a minimum dis tance from the wall of 10 cm 4 inches at the back and of 15 cm 6 inches on each side must be kept 13 Before use e The centrifuge must be protected from heat and direct sunlight e The location must be well ventilated at all times UV rays reduce the durability of plastics Protect the centrifuge rotors and plastic accessories from direct sunlight 14 Main connection Connect the centrifuge only to a grounded main power supply Make sure that the power cord is compatible with the valid safety regulations and that your main voltage and frequency correspond to the specifications on the instrument label Make sure that the centrifuge is switched off on the right hand side of front panel before connecting electrical wire to main power supply 4 gy TW RS232 interface specifications Main connection Rotors and accessories Rotors and accessories A rotor is not included as part of a Multifuge centri fuge A large variety of rotors are available as accessories In addition there are adapters and reduction sleeves for a variety of commercially available tubes and bottles For a complete list of available tubes bottles adapt ers and accessories please refer to our current sales documentation For more information you can visit our web site at http www Kendro com Multifuge 1S air cooled During cent
52. stop using it Corrosion protective oil 7000 9824 is delivered with the centrifuge Maintenance and care The KENDRO service offer Kendro Laboratory Products recommends annual ser vicing of the centrifuge and the accessories by author ized customer service or trained professionals The customer service personnel inspect e the electrical installations e the suitability of the location e the lid lock mechanism and the safety circuit e the rotor e the rotor fastening and the drive shaft Defective material is exchanged KENDRO offers inspection and service contracts cov ering it Inspection costs are charged as flat rate con tracts Necessary repairs are carried out free of charge within the warranty conditions and requires payment after expiration of the warranty period Warranty conditions The warranty period starts on the day of delivery Within the warranty period the centrifuge is repaired or replaced free of charge if there are provable faults in materials or workmanship Conditions for a warranty are e the centrifuge is used according to the instructions of use e mounting extensions settings alterations or re pairs are carried out exclusively by personnel au thorized by KENDRO e the required maintenance and care procedures are carried out regularly 57 Maintenance and care Notes 58 Troubleshooting Troubleshooting Proceed as follows E lid rel 1 Make sure that the
53. struments and can process nominally 1 6 liters of sample in a single run There are various rotors available that can achieve high RCFs and accommodate a wide range of accessories for all common tube types micro titer and deep well plates The user friendly EASYset control panel permits easy selection of speed RCF value run time and run profile acceleration and deceleration as well as temperature of the Multifuge 1 S R You can switch from speed to RCF display and vice versa with a touch of a button and even during a run Description of the Multifuge Safety systems The Multifuge is equipped with a number of safety systems Housing and lid are constructed of 6 mm steel Lid with window Lid lock with safety check You can only open the centrifuge lid when the power is turned on and the rotor has come to a stop You can only start the centrifuge if the lid is properly locked Automatic rotor identification Electronic imbalance detection as a function of rotor SMARTspin Do not tamper with the safety systems Parts supplied Accessories supplied with the centrifuge are power cord a special wrench for securing the rotor As seen in the picture condensed operating instructions corrosion protective oil The printed documents consist of the delivery notes and this Manual Description of the Multifuge Function and features Program memory Data are stored until new values are
54. tinued Following onset of deceleration you must wait until the rotor has stopped By opening and closing the lid you reset the message rotor After entering a permissible speed you can press start Lid not properly closed Open the lid and repeat locking procedure Lid was opened manually during the run The instrument stops without deceleration If you want to continue the run you must switch the instrument off and on again The lid has not been unlocked after release 1 Avoid laying objects onto the centrifuge lid 2 Lift the lid slightly 63 Troubleshooting 64 Symptom Rotor stops without deceleration to standstill Instrument cannot be operated Rotor stops with deceleration to standstill Instrument cannot be operated Rotor stops with deceleration to standstill Instrument cannot be operated Rotor stops with deceleration to standstill Instrument cannot be operated Instrument stops with deceleration to standstill after short starting up Possible causes and corrective measures Internal program error Switch the instrument off and on again If the error persists contact KENDRO service Overtemperature in the centrifuge tank Switch the instrument off and on again If the error persists contact a KENDRO service station Temperature measurement error Switch the instrument off and on again If the problem persists contact a KENDRO service station Max
55. tion profiles 7 decontamination 54 Description 7 Description feature 9 Diagnostic messages 11 disinfectant 55 disinfection 4 procedure 55 disinfection with bleaching lye 55 displays brief failure 61 E EASYsetTM user interface 10 EASYset control panel 7 Emergency lid release 59 Entering parameters 43 Entering changing a program 50 error codes Lid 63 OPEN with lid closed 63 rotor 63 94 error codes DAI 62 E 01 E 40 64 F filling the centrifuge tubes 40 fixed run time 45 G general view 7 8 15 16 18 19 20 22 23 goes forward 48 H handling the centrifuge 4 hazardous substances 3 hazards symbols 5 hints symbol 5 hLd 45 46 icons for denoting dangers and potential damage 5 imbalance 40 41 imbalance 48 imbalance detection 8 indoor use 13 infectious material precautions in case of tube breakage 54 Inserting the centrifuge tubes 41 Installing the rotor 38 L lid blockage 61 Lid lock with safety check 8 lid opening 37 lid release Emergency 59 lid unlocking mechanism manual 59 limited life time 40 location 13 maintenance 53 manual lid unlocking 59 manual stopping 49 max tube dimensions 25 maximum filling volume 35 Maximum loading 39 maximum permissible filling volume 34 maximum permissible RCF 48 maximum permissible speed 48 Maximum RCF va
56. tronic imbalance shut down is activated Operation Inserting the centrifuge tubes Fixed angle rotors The rotor must be loaded symmetri cally Failure to do so can cause rotor imbalance which may lead to noisy operation affect separation quality or result in imbalance detection shut down as well as introduce significant detrimental wear to the motor and drive system When only partially loading the rotor ensure that oppo site bores receive tubes of equal weight when centri fuging a single sample place a centrifuge tube filled with water opposite of the sample After placing the tubes install the rotor cover Improper loading 41 Operation Swinging bucket rotors Proper loading 42 E Improper loading These examples are to be applied to the other rotors in an analogous manner Operation Entering parameters Deceleration curves The Multifuge offers 9 acceleration and deceleration profiles for optimal centrifuging of samples and gradi ents Please consult the diagrams in the Appendix for more detail of the acceleration and deceleration curves for rotors not mentioned here you may extrapolate the respective values After switching on the centrifuge the centrifugation profiles last entered are selected By pressing the set key IS you can scroll through the profile settings until the desired profile is reached Once the display stops flashing the value is stored in
57. version as follows 1 Switch the instrument off 2 Keep selection key pressed and switch on the instrument For about 1 sec all displays read 1 DI DIS SS MULTIFUGE 1 sn Hermeeus 70 Subsequently the following readings will be dis played for 5 seconds each Numbers are examples Software version keyboard _ 526 _ 2 Software version _ 528 _ 6 NV RAM version 4468 _7 The value in the time panel indicates the development stage The last information displayed indicates the current cycle status Cycle counter 235 CY The values given are only examples During the subsequent program test the message _ TEST PRO 4 1 is displayed only for instruments with refrigeration system Technical Data Technical Data Ambient conditions indoor use maximum elevation 2000 m 6562 ft above sea level max relative humidity 80 up to 31 C 88 F linearly decreasing down to 50 relative humidity at 40 C 104 F Ambient temperature allowed 2 C to 40 C 36 F to 104 F run time standard mode 1min 9 h 59 min hold permanent extended mode 1min 99 h hold maximum speed Nmax 15 000 rpm rotor dependent adjustable in steps of 10 minimum speed nmin 300 rpm 24652 micro liter rotor 48 x 2m 7500 3348 maximum kinetic energy Multifuge 1S 34 3 kNm 25 298 ft lb Multifuge 1 S R 34 3 kNm 25 298 ft Ib noise at maximum speed Multifuge 1S lt 64 dB A Multifuge 1 S R lt 54 d
58. you switch from actual to set point values The value last stored is displayed with the digit entered flashing if there is no value stored in memory this is indicated by dashes 2 By briefly pressing the input key you can raise or e lower the speed by oO one step 10 rpm at a time 3 If you hold down the pressed key the display changes slowly at first and at an accelerated pace after a few seconds 4 Release the key as soon as you have reached the desired value and adjust if necessary by repeatedly pressing the key The decimal place flashes for a 44 number of seconds then changes to a permanent display The speed is now stored E For faster operation you may shift the flash ing cursor in the speed RCF and the run time panels just press both S and DM si multaneously The cursor moves by one digit to the left for each time the key is pressed Entering the RCF value You can adjust the RCF set point in steps of 1 The set point is entered analogously to the speed As long as the rotor has not been identified it is not possible to display RCF values This is signaled by dashes in the display Shortly after starting the centrifuge run the rotor is identified and the current value is displayed NOTE If you set an extremely low RCF value this may be automatically corrected if the resulting speed would be lower than 300 rpm Operation Mo
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