LabTurbo 48-Compact user manual HV 2014 May
Contents
1. 6 1 Barcode 1 For reagent barcode recording select Barcode Menu Barcode Sampling 82 3 Extraction PCR setup O Turn off 2 Please scan the barcode on reagent bottle first and then scan the barcode on the Clean associated buffer reservoir Once they are matched the screen will display the color and position to help users recognize the correct reagent reservoir It also records the refilling date reagent lot number kit lot number and extraction kit expiration date Repeat the steps to refill all buffers for extraction LabTurbo_48 Compact Barcode Reading LabTurbo_48 Compact Barcode Reading Input the Bottle Barcode Input the Bottle Barcode KeyBoard KeyBoard DLL LTL CCEB DLL LTL CCEB ETOH LWI PR ETOH PR dCCEB dCCEB 11 152013 11152013 11152013 11152013 11152013 102472013 485136263444 487020031201 483335111101 482111011203 481136263604 486 not in use 11152013 11152013 11152013 11152013 485136263444 487020031201 483335111101 452111011203 10242013 486 not in use Kit Lot No 48074011412 Exp Date MM YYYY 12 2015 New Save Back kit Lot No 45074011412 Exp Date MM YYYY 122014 New Save Back 13 3 The reagent barcode information is automatically saved in the system To obtain an independent reagent barcode documentation file please select Save to export the data The saved data format is txt file ESE Compact Barcode Reading Choose you2. Sample Barcode 748 Thermo rack Sample no 45 Check 45 collection tubes on the Thermo block t t ke D B Ki 1 Di v E Di H m vu m D m H 5 E D m D Sample Barcode Save New Thermo rack Sample no 48 Check 48 collection tubes on the Thermo block Confirm 4 2 For elution tube barcode recording please select barcode before tapping the elution tube section on the screen The barcode input sheet will display Follow the highlight in the yellow to scan the associated barcode on each elution tube Once a barcode is keyed in and matched it will be marked in blue To modify the barcode select the barcode to be modified and key in the correct barcode Elution Barcode 4 o KeyBoard Elution rack Sample no 6 Check 6 collection tube s in elution rack and elution cover on elution rack Elution rack Sample no 6 Check 6 collection tube s in elution rack and elution cover on elution rack Back Next Skip Iesel Back 20 5 Remove all tips left from the last run Put three new full 96 well tips 1100 ul on Tip rack 1 Tip rack 2 and Tip rack 3 8 Carefully place 6 strip 2 0 ml sample tubes with samples on the Sample lysis thermoblock one by one For Sampling Extraction function please refer to section 6 2 to put the primary tube rack into the machine and
3. Open the door take out all the columns and use a centrifuge for elution Close the door and click OK to finish the extraction procedure Low elu vacuum try again l 2 Open the door and make sure there are no missing columns on the Elution cover Close the door and cl ck Yes to continue If this message remains please click No Another message will show up Take row No X columns out and used spin down to elution X represents the row numbers of elution cover The row number could be No 1 8 Open the door take out the columns and use a centrifuge for elution Close the door and click OK to continue Run Time error This message indicates that the system is overloaded and the program will terminate to prevent machine from doing anything wrong Once this message pops up please record the current step first This is very important because once ok is clicked the program will terminate automatically To finish the whole procedure users can manually recover the worktable restart the program and then continue from the terminated step to complete the extraction The worktable should be recovered manually to the original status as the following steps l Clean all remaining 6 strip sample tube column set and elution tube on the worktable 2 Replace a new 6 strip tube on HCCEB rack Vending plug aging replace a new vending plug at EtOH LW
4. 11 For adding only Template to PCR tubes select Elution Only PCR Setup Master Mix Elution Elution Only Back 12 Define the sample number and choose the PCR tube position Al B1 Cl DI El Fl Gl and H1 Define the elution volume and click Next Elution Only Master Mix I Master Mix 2 Sample No 6 PCR Tube Position Master Mix Volume Elution Volume Export data Next Back 13 Follow the PCR setup loading check PCR setup loading check Fill up Tipl Rack with 300ul tips Fill up Tipl Rack t Back 30 14 Follow the instructions and fill up Tip rack 1 with 300 ul tips place PCR tubes plate or strip on the 96 well PCR tube plate adapter then mount the assembly on Tip rack 2 2665 eeesesss e eeegeee eg Sg gege eee sees sg ore eeeeesees sasnaanaasasss ereeeesasase PCR setup loading check Fill up Tipi Rack with 300ul tips Place PCR tube plate adaptor on Tip2 rack Volume information of Template v IC lose the door and start Start Pause Next Back 15 Close the safety door and press Start 16 When the operation completes the System Message will pop up system Message AJ Program completed 3l 6 5 Clean Select Clean to enter clean and maintenance page Menu III dt Barcode Sampling 82 Extraction PCR setup l Turn off Reservoir Clean 1 Check the waste level i
5. ZN WARNING Used plastic wares such as filtered tip and Eppendorf tubes may have caustic chemicals or biohazardous reagents remained Such waste and disposals must be properly collected and disposed in accordance with the local safety regulations vi 2 7 Mechanical hazard ZN WARNING This machine has a safety door Once the power of LabTurbo AN Compact is on the user should not stretch into the workstation under any situations Ncaurion Please confirm that the metal rack for reuse tips is empty before using If there is any tip left before using please press Reset 2 8 Heat hazard WARNING The Thermoblock of LabTurbo 48 Compact can heat up to 80 C Be aware of it when the heating step is undergoing It is not allowed to use the Thermoblock with volatile or flammable liquids WARNING The maximum continuous operating time of the vacuum pump is 1 minute to avoid overheating 2 9 Electrical hazard Z WARNING Modification to any electrical components wires cables circuit boards etc of LabTurbo 48 Compact by unauthorized personnel is prohibited The opening of covers or removal of parts is likely to expose dangerous voltages Disconnect the machine from all power sources before handling electrical parts of the machine vil EXTERNAL FEATURE LabTurbo 48 Compact is the automated nucleic acid purification system with innovative vacuum membrane column technology The externals of the main
6. SE Next Back 4 A Loading check window will pop up Follow the instruction on the window to set up the worktable For sample barcode documentation function tap Barcode Tu HH iu NIE Thermo rack ODOOODOO 0609000000 7OCOOCO 00090009 C0COOOO00 Sample no 24 3 Check 24 collection tubes on the Thermo u p UT amm block Back Next Skip W 5 Use the barcode reader to scan the barcode on each primary tube The barcode will be automatically entered and show on the screen Follow the highlight in yellow to scan the barcode on the associated primary tube Once barcode is entered it will be marked in blue If a duplicated barcode 1s keyed in it will be marked in red To modify the barcode data select the barcode to be modified and key n the correct barcode Sample Barcode 72 Sample Barcode 5 New keyBoard 4 New KeyBoard Thermo rack Thermo rack Sample no 24 Sample no 24 Check 24 collection tubes on the Thermo Check 24 collection tubes on the Thermo block block Back Back 16 Once all barcodes are keyed in select save to export a txt documentation file of the barcode then select confirm to continue to the next step To redo the barcode input step select New to refresh the barcode input sheet and key in all barcodes again Check the box in front of keyboard to manually key in sample barcode Sample Barcode 24 Thermo rack Sample no 24 Check 24
7. collection tubes on the Thermo block 7 Close the safety door Press bed to start the protocol 8 When the sampling function is finished the System Message will pop up Open the safety door to check if all the samples are transferred to 6 strip sample tubes and take out the primary tube rack Go back to the main menu to start extraction procedure System Message iJ Program completed 6 3 Extraction 1 For DNA RNA purification select Extraction Menu III ul Barcode Sampling 82 Extraction 3 PCR setup O Turn off M 2 For extraction starting from 6 strip sample tubes select Extraction For extraction starting from primary tubes with automatic sampling function select Sampling Extraction LabTubo_48 Compact Extraction Protocol Extraction Sampling Extraction User Name C Barcode Digit 12 9 Back KeyBoard 3 Select the sample numbers and the protocol e g HV LGD3 In300 E100 V7 0E click Next LGD means the kit for extraction In300 means the sample input volume is 300 ul E100 means the elution volume is 100 ul Extraction Protocol Sample No Waste level Svringe status Breet 24 Safe 500 HV LGD3 In300 E100 V7 0E pro Next Back 4 A loading check window will pop up Follow the arrow at each step to check correct worktable setup For recording barcodes of sample and or elution tubes please f
8. system includes a Waste bottle for major waste collection during binding washing and cleaning steps with liquid level detection function and a Trash can WORKTABLE UNITS 1 Ultrasonic fluid sensor 2 6 Channel spacing adjustable pipette 3 Plug tube rack 4 HCCEB tube rack 5 96 well tip rack 6 Proteinase K tube rack and PCR master mix tube rack 7 6 x 8 48 well Sample lysis thermoblock 8 Reuse tip rack and tip disposal vent 9 Vacuum plug set 10 Binding washing vacuum manifold 11 Buffer tank 12 Elution vacuum manifold Tip3 Tip2 4 1 Six channel spacing adjustable pipette The pipette is equipped with 6 syringes and has adjustable tip spacing 9 mm and 18 mm suitable for liquid transfer between microcentrifuge tubes and PCR tubes The working volume of the pipette is 5 1000 ul The standard tip is the 1 1 ml filtered tip I F i l 4 2 Ultrasonic fluid sensor The ultrasonic fluid sensor detects any clogged membrane after the steps of lysate binding and buffer washing In addition it functions to check if the buffer is sufficient for the extraction protocol and measures the sample level one by one in the primary sample tube 4 3 96 well tip rack Three 96 well tip racks from front to rear are Tip 1 Tip 2 and Tip 3 are for three 96 well plate tips which can be 1100 ul tips for extraction or 300 ul tips and 96 well PCR tube plate adaptor for PCR setup The metal pi
9. the PCR barcode by the import function barcode scanner or keyboard If the barcodes are entered during Extraction see section 4 1 4 2 it will automatically display on the sheet Select Renew to clean up the existing barcode Select Add New for adding new barcode or select Import for adding barcode defined n a txt file Choose Next to proceed PCR Setup PCR Setup PCR Plate Barcode PCR Plate Barcode User Name KeyBoard User Name KeyBoard Elution Barcode No l Elution Barcode Nal Import Renew Add New kevBoard Import Renew Add New kevBoard as H DI DI ai a as ELI 4 A a E 5 LE at A42 s Li M i a aM A as M a a 2 MH as KI I 27 ki Am E f Mm A m D RI d 22 23 24 m 2 22 2 2 ts 14 is 14 1 18 13 14 b6 17 18 5 9 Ie 1 12 4 B 5 to 1 12 Next Back Next Back Specify the sample number and select the PCR tube position Al Bl Cl D1 El Fl Gl and Hl to define the starting position on the 96 well PCR tube plate adapter to dispense the template and the master mix M1 stands for the first master mix and M2 means the second master mix Master Mix and Elution Master Mix 1 Master Mix 2 Sample No PCR Tube Position Master Mix Volume 20 ul Elution Volume I0 ul Export data Next Back Define the master mix and elution volume for PCR preparation Then choose Next to proceed Master Mix and Elution Master Mix 1 Master Mix 2 Sample No 6 PCR Tube Position Al A6 Master Mix Volume Elut
10. CEB EtOH and LWI1 Note Buffer label may vary from actual condition 3 5 Label The label consists of the product name model and power rating The serial number with the barcode and the manufacturing date of the product are also mmm EO ce TAIGEN Biouceoce pem er conn te x E 886 2 2B49 i FAX 866 2 28836458 Mat orcenfbusgen com included 3 6 Side panel The side panel is located on the right fa ade of the machine It contains the following A LAN Control is for Internet connection or the remote control B USB Accessory is for the peripheral with the USB port such as the mouse keyboard or USB flash drive C AC 110V 220V and ON OFF are the main power switch and the power cord socket The fuse chamber is in between the main power switch and the power cord socket NOTE Voltage values on all pictures are for demonstration purpose only Each machine s actual supply voltage may vary depending on its module Tun Ben en g T9 UNS e 3 ewm a aem weg 1 mm LLL LLL LL V SL AC 220V LAN Control USB Accessory Ai Ei E 3 7 Machine feet with wheels The height of the machine feet is adjustable and accompanied with the wheels Manage the level of the workstation by adjusting the height of the machine feet The wheel is only for short distance movement of the workstation on the table but not for transportation of the machine on the ground 3 8 Waste system The waste
11. I position This message will pop up after the extraction is completed User could not perform 51 the next run of extraction or sampling function until a new vending plug 1s replaced Menu 5 mm Barcode 3 PCR setup O Clean Turn off 1 Check the number on the top of vending plug Prepare a new vending plug cC LW1 aH m 2 Pull out the old vending plug and replace another new vending plug immediately Caution Reagent will flow into the reagent tank when the vending plug 1s pulled 3 Push the new vending plug to the bottom Turn off LabTurbo software and turn on again for the next run of extraction X or Z or Y Axis position error Please contact our customer service for more information and support 52 Taigen Bioscience Corporation 3F No 150 Sec 4th Cheng De Road Shi Lin District 111 Taipei City Taiwan Phone 886 2 28891136 Fax 886 2 28836458 Website www labturbo com Email order taigen com 53
12. Laniurto COMPACT SYSTEM ADVANCED AUTOMATION FOR DNA RNA PURIFICATION 2014 05 PREPAC EE iv 1 1 Purpose OF this manual RE iv SAFETY PRECAUTION gt ee ere ere ee iv 2 1 Veit UR naar v 2 2 Equipment assembling carrying and positioning requirements v 2 3 Ventilation requirements a ana vi 2 4 Additional equipment positioning requirements vi 2 5 Explanation of safety marks on the equipment vi 2 6 Biological Safety ss een vi 2 7 Mechanical hazard nn vii 2 8 Heat hazard a ihn vii 2 9 Electrical Hazard anni vii EXTERNAL FEATURE P Et Yet Ee tei aes fm Para ege 1 3 1 Built in touch Screen u ae 1 J2 SIEV Le TEE 1 3 3 POWER ann 2 3 4 Automatic reagent feeder uuuuuuununnununnnnnnnnnnunnnnnnnnunnnnnnnnnnnnnnnnnn 2 3 9 Lab a a nee 2 326 SIGE Dancehall 3 3 7 Machine feet with wheels zsssssnnnnonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 3 3 0 Waste SYSTEM ci 4 WORKTABLE UNITS css a 5 4 1 Six channel spacing adjustable pipette 6 4 2 Ultrasonic fluid sensor aus in 6 4 3 96 Well LID TACK sinn 6 4 4 P1Ug tube di 7 4 5 HCCEB tube Track 7 4 6 PCR master mix tube rack ai 7 4 7 Proteinase K tube rack uuusuunununnunnunnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 7 4 8 6x8 48 well Sample lysis thermoblock 8 4 9 Vacuum DIUG Sek a ei 8 4 10 Tip reuse rack and tip d
13. a device manufactured and assembled in the certified factory There are no special requirements for the installation of the device While carrying the machine make sure the passageways are clear of all obstructions Instruction must be received prior to any lifting applied A CAUTION The device should be placed and anchored securely on a sturdy pedestal or platform 2 3 Ventilation requirement N caution This device is equipped with a ventilation fan that allows the air to circulate and removes stale air Therefore no additional environmental measurements are required A lab with appropriate ventilation is fine 2 4 Additional equipment positioning requirements J WARNING The main power cord of the device is on the right side of the equipment Be sure to keep the right side of the device at least 15cm from the wall or other obstacles 2 5 Explanation of safety marks on the equipment All of the signs on the equipment are marked according to the standard of IEC EN UL 61010 2 6 Biological safety J WARNING Assorted biological solutions and specimens from humans or animals carcasses tissues body fluid etc should be treated as potentially infectious The users must strictly follow waste disposal guidelines or consult the safety officer with regard to an appropriate method for disinfection ZN WARNING Handle potentially biohazardous samples with the greatest care and in accordance with the required safety regulations
14. achine will continue the extraction procedure Please fill Tip 1 2 3 Rack and install it correctly This message indicates that there are missing tips in one or more tip racks Please open the door and fill up the tip racks After refill close the door and select OK Remember to install all three tip racks for each run of extraction Please add XXX buffer X Xml into the buffer tank X This message indicates that the amount of a buffer 1s not enough n its Reservoir Please open the door to refill the buffer over the refill line After refill close the door and select OK XXX represents LW1 CCEB DLL Proteinase K etc 43 Open the cap of proteinase K This message indicates that the cap of proteinase K vial is not opened Please remove the cap of proteinase K vial put t back onto the rack and then close the door to start the procedure M1M2 PK Please add PK XXX ul into the enzyme bottle This message indicates the amount of Proteinase K is not enough Take out the Proteinase K v al and replenish it Put the v al back and close the door then select OK to resume the procedure Please add LW1 XXml into the buffer tank This message indicates that the amount of buffer is not enough in the reservoir Please open the door to refill reagent over the refill line After refilling close the door and select OK Missing elution cover put it on elution
15. cates the selected protocol for the procedure 3 The Timer shows elapsed time or remaining time count down It also indicates the total and current steps of the protocol 4 The Monitor indicates the current heater temperature and vacuum pressure of the machine 5 The Platform Configuration mirrors the worktable to the machine The green icons indicate the operating units at the current step 24 ME Live Pause Start Reset Menu 1 Live Click this button to initiate the camera to show the real time view of the worktable 2 Pause Click this button to pause the machine same as opening the safety door 3 Start Click this button to start the procedure 4 Reset Click this button to reset the machine and move the robotic arm to original position 5 Menu Click this button to go back to the main menu 23 6 4 PCR Setup 1 For PCR preparation select PCR Setup Menu ILLI nnt Barcode Sampling 82 3 PCR setup Extraction O Clean Turn off 2 Select the type of PCR strip tube Note The PCR setup operations are different for 8 and 12 strip tubes LabTurbo_48 Compact PCR Setup mA EA EA LAC 2825 ts Be See Se Bonen ow 12 Strip Back 3 For adding master mix elution template to PCR tubes select Master Mix Elution LabTurbo 48 Compact PCR Setup Master Mix Elution E Elution Only PCR Tube Plate Barcode keyBoard Next Back 26 Add
16. ic reagent feeders and therefore require no manual input of buffers After the extraction the remaining buffer in the buffer tank is automatically drained out On the back of the buffer tank 1s the place for placing the vacuum plug set 4 12 Binding washing vacuum manifold Both binding and washing steps are performed here This 1s the unit for applying spin column adapters spin columns and vacuum plugs Up to forty eight spin columns can be placed on this unit Connected below is the stable vacuum system The extra wells are for the accommodation of 7 vacuum plug sets 4 13 Elution vacuum manifold This unit is for 48 product elution Eight airlocks located on the right side of the elution block are used to control each elution row A plastic handrail with magnets comes with the metal cover of the elution block for convenient handling of the cover ACCEEORIES 5 1 Elution tube 6 strip elution tubes 2 0 ml 1 5 ml Eppendorf tube with caps or 2 0 ml Eppendorf tube with caps are placed on the Elution vacuum manifold for elution product collection 5 2 Sample tube 6 strip sample tubes 2 0 ml are placed on the 48 well Sample lysis thermoblock The 6 strip caps can be used for sample preservation in the 6 strip tubes 5 3 Column set The column set consists of spin columns and 6 strip spin column adapters which is placed on the Binding washing vacuum manifold for genomic DNA RNA and v
17. ion Volume Export data wf FP Next E ac 7 PCR setup loading check page will pop up Follow the instructions to fill up Tip rack 1 with 300 ul tips place PCR tubes plate or strip on the 96 well PCR tube plate adapter then mount the assembly on Tip rack 2 PCR setup loading check m A r Place PCR tube plate adaptor on Tip rack r Volume information of Template r Close the door and start Note The PCR setup operations are different for 8 strip tube and 12 strip tube For 8 strip tube format refer to the upper label for the tube positioning For 12 strip tube format refer to the lower label for the tube positioning zo nm OR IR 9 10 11 12 8 The required volumes of master mix and template are indicated on the loading check page Apply sufficient amount of master mix on 2 0 ml screw tube and put 28 it on the master mix rack Ensure that the elution tubes are placed on the associated positions on the elution rack PCR setup loading check Volume information of Standard Master Mix Template Template Vol ol 48 gt M ul Master Mix Vol Back PCR setup loading check S Fill up Tipl Rack with 500ul tips Place PCR tube plate adaptor on Tip rack 10 When the operation completes the System Message will pop up System Message Eq Program completed 29
18. iral DNA RNA extractions 5 4 Plug tube combination Five orange plug tubes are analogical to the column set and are mounted on the Binding washing vacuum manifold to secure vacuum pressure during vacuum application They are used only for the last row of CPU where sample extraction takes place e g the row of sample No 7 The machine will setup the plug tube combination automatically during the extraction 5 5 PCR setup plate adaptor For PCR setup function put PCR tubes or plate on the plate adaptor and apply them to the Tip rack 2 of the machine It can accommodate 8 stripped 7 T m D o u 12 stripped or 96 well format tubes 2352454 1 SOFTWARE INTERFACE AND OPERATION Main menu After machine startup the computer will automatically launch the LabTurbo 48 compact system software and display the main menu page The main menu includes the options of all LabTurbo functions Users can easily select the desired function to start the operation Barcode for reagent barcode tracking and documentation Sampling for transferring sample from primary tubes or 2 0 ml screw tubes to 6 strip sample tubes Extraction for nucleic acid extraction procedure PCR setup for liquid handling PCR setup or sample transferring Clean for machine maintenance and UV sterilization Turn off for turning off the machine Menu Barcode 82 Extraction Sampling 3 PCR setup Turn off
19. isposal vent 8 4 11 Buffer tank ak 9 4 12 Binding washing vacuum manifold 9 4 13 Elution vacuuni manifold ass 9 ACCBEEORTES coco Ente a uina CUP RU Edd eU MM RE 10 5 1 EIERE Eege hg Eege eeh 10 5 2 Sample CUD EE 10 5 3 Column SeE EE 10 5 4 Plug tube combination ana 11 5 5 PCR setup plate adaptor u 11 SOFTWARE INTERFACE AND OPERATION 00000aaaaaonnnnsnsnennesssssereessssree 12 6 1 ge TEEN 13 CAI emt 15 6 3 EXtractio Binissiciiesteiunsnneniseriwsen cumini ene I 18 6 4 PCR SC TU e ME 26 6 5 CIO AN dd 32 MAINTENANCE nee ee 37 7 1 Daily clean and maintenance e eere eene nnn nnne 37 7 2 Weekly clean and maintenance eee eene eren nnn nnn 38 7 3 Monthly clean and maintenance KEE nennen nnn 39 TROUBLE SHOOTING oo hr rn ea Ex Saas ERES RR VERE In erunt 41 iii PREFACE 1 1 Purpose of this manual This operation manual is written for laboratory personnel and researchers who conduct automated sampling barcode reading DNA RNA extraction and PCR setup using LabTurbo 48 Compact System LabTurbo 48C SAFETY PRECAUTIONS This manual contains information and warnings that must be followed by the user to ensure safe operation of the LabTurbo workstation and to maintain the instrument in a safe condition Potential hazards that may harm the users or re
20. manifold correctly This message indicates that the elution cover is missing Put the elution cover back correctly then click OK to resume the procedure 44 Take off carrier of Elution cover This message indicates that the handrail for elution cover was not removed Remove the carrier from elution cover then click OK to resume the procedure CM Elution cover on wrong direction put it on elution manifold correctly This message indicates that the Elution cover is placed in the opposite direction The Front label should be placed toward the user Remove the excessive column SC adaptor or Plugset at sample This message indicates that the worktable setup at Vacuum manifold is incorrect Take the worktable setup of 6 samples as an example an additional column set 1s misplaced at position 7 left picture Only 6 column sets should be placed at Vacuum manifold right picture represents the sample position at Vacuum manifold or 7 in the aforementioned example 45 This message also indicates the Plugset is at the incorrect position For example six plugsets are set at the incorrect positions on the left picture Please refer to the Monthly clean and maintenance section section 7 3 for the plugset setup instruction Ten T a a ES E we Te Te A ee E IL Removing the excessive SC adaptor on the vacuum manifold This message indicate
21. n on the lower right of the rack top is a foolproof feature for mounting the tip plates 4 4 Plug tube rack The plug tube rack 1s to accommodate five plug tubes 4 5 HCCEB tube rack The rack is for 2 0 ml 6 strip tubes the tube is the same as 6 strip sample tube of hot column clean elution buffer HCCEB in the extraction procedure 4 6 PCR master mix tube rack The rack is for two master mixes M1 M2 used in PCR setup 4 7 Proteinase K tube rack The rack 1s for 6 ml screw capped tube of enzyme proteinase K for extraction 4 8 6x8 48 well Sample lysis thermoblock The Sample thermoblock is the 48 well block for sample lysis and drying column It can heat up to 80 C for heat incubation The notch on each row is a foolproof feature for placing the 6 strip sample tube 4 9 Vacuum plug set 6 strip vacuum plugs are used on the binding washing vacuum manifold The function of the plugs is to prevent vacuum leakage during vacuum application Those Plugs must be placed on the left position as shown in the picture below 4 10 Tip reuse rack and tip disposal channel Reusable tips for wash buffer and ethanol are placed on the tip reuse rack The tip disposal channel on the left side of the tip reuse rack 1s the channel for disposal of tips and consumables 4 11 Buffer tank Five buffer tanks are for lysis buffer EtOH washing buffer and elution buffer CCEB All buffer tanks are connected to automat
22. n the Waste Bottle and drain out all the waste 2 Select Reservoir Clean Vacuum UN Sent Thermoblock Waste Level x Safe Start Pause Back 32 3 Check the Reservoirs intended to be cleaned Clean VLL CCEB LWI DLL BYE acces BtOH ERWI Minutes Process Waste Level Safe Start Back 4 Close the door and press Start The machine will automatically drain out the remaining buffer to Waste Bottle 5 After finishing the procedure please drain out all the waste in Waste Bottle again 6 Select Reservoir again at the clean page and check the same reservoirs on step 3 add ddH20 to Reservoirs for cleaning 7 Close the door and press Start The machine will automatically drain out the ddH O to Waste Bottle 8 After finishing the procedure please drain out all the waste in Waste Bottle 33 Tank Clean 1 Check the waste level in the Waste Bottle and drain out all the waste 3 Select Tank Vacuum UV Light Thermoblock Reservoir Waste Level Safe Start Pause Check the tanks intended to be cleaned Clean DLL VLL CCEB EtOH LWI M Minutes Process Start Pause Back 34 5 Close the door and press start The machine will automatically drain out the ddH5O to Waste Bottle 6 After finishing the procedure please drain out all the waste in Waste Bottle Vacuum and thermoblock clean 1 Select Vacuum Thermobl
23. ock Vacuum UV Light Thermoblock Reservoir Tank Waste Level z Safe Start Pause 2 Place one 1100ul 96 well tips on Tip rack 1 and add 180 ml ddH2O on Tank 5 iene XE st 4 e ur tr Nir Geb bP OI i n h E t 4 Pir tw Sr 2 pe io L e nn f 1 hte So OSG amp Eh j A H ber irr E eL tn gt Se 15 Wa T a ni 7 gt e fy I e Za r 7m d ev P l 4 Follow and check the instructions on the screen and click Start Clean Vacuum Thermoblock Add dH20 200ml into the Tank 5 Fill tips in the Tip rack 12 Minutes Process Waste Level Safe Start usse Back UV light clean 1 Select UV light d Vacuum Uy Sent Thermoblock Reservoir Tank Waste Level Safe Af A 2 Choose the UV light duration and click Start Clean UV Light UV Light 30 Minutes 30 Minutes I Process 30 60 Waste Level Waste Level I 20 Safe Safe Back 180 36 MAINTENANCE 7 1 Daily clean and maintenance 1 Collect all the tips left on the Tip racks for the next run 3 Wash the Elution Cover with tap water and air dry or wipe and dry with tissue paper Do not put in Oven or autoclave to dry 4 Check the level of the trash can 37 5 Check the waste level in the Waste Bottle and drain out the waste 7 2 Weekly clean and maintenance 1 Add distilled water to each trough
24. of Reagent tank 2 Select clean tank function and choose the tank for cleaning Clean Clean rDLL VLL e CCEB EtOH LWI Vacuum ERR Thermoblock 4 Minutes Process D Weser arr Pau Start Pause Back 3 Replace 6 strip tubes in HCCEB rack with a new one 38 4 Check the level of waste bottle and trash can 7 3 Monthly clean and maintenance 1 Clean each side of machine by using hand towel 2 Check the level of waste bottle and trash can 3 Choose clean and select Vacuum thermoblock Add 180 ml distill water into Tank 5 Place one 96 well tip on Tip 1 rack Clean gt Vacuum UV Eight Thermoblock Reservoir Tank Waste Level gt Safe Start Pause Back 39 4 Optional Wash Vacuum plug sets with tap water and air dry or wipe and dry with tissue paper Make sure to put seven Vacuum plug set back to the original positions Six Vacuum plug sets should be placed on the left side of the Binding washing vacuum manifold One Vacuum plug set should be placed on the rear side of machine behind reagent tank 40 TROUBLE SHOOTING DAO connection error software run the Demo Version This message indicates that the controller fails to connect to computer Please turn off the LabTurbo 48 Compact software and the machine After all are turned off switch the main power off and on
25. ogram if one or more buffer in the Reservoirs is stored over three months Select OK to continue Please refer to section 6 1 to replace the expired buffer with a new one XXX represents LW1 CCEB DLL Proteinase K etc LabTurbo 48 Compact Barcode Reading Input the Bottle Barcode KeyBoard DLL LTL CCEB ETOH LWI PR dCCEB 111152013 11182013 11152013 11152013 11152013 10242013 48516263444 487020031201 483335111101 482111011203 481134626604 486 not tn use Kit Lot No 48074011412 Exp Date MM YYYY 12 2015 New Save Back Close the door This message indicates that the door is opened Check the sensor on the right side of the door and make sure the door is closed Please close the door and start the procedure again If this message remains turn off the computer switch the main power off and on and then restart the computer 42 Windows error CCD connection failed turn off the main power and restart the system This system message indicates that the CCD camera is not successfully recognized by the system computer through the USB interface Please turn off the software and restart the computer to let the computer recognize the CCD camera Wrong plug tube setup This message may show at the beginning of the extraction Check if the five plug tube combinations are at the original positions If the plug tube combinations are set up correctly click Cancel and the m
26. ollow step 4 1 and 4 2 oH Amt N in NNN Tip rack Sample no 48 Please fill up tips in Tipl Tip2 and Tip3 racks 4 1 For sample barcode recording please select barcode before tapping the sample tube section on the screen The barcode input sheet will display Follow the highlight in yellow to scan the associated barcode on each sample tube Once a barcode is keyed in it will be marked in blue If a duplicated barcode is keyed in it will be marked in red To modify the barcode data select the barcode to be modified and key in the correct barcode c Y Emm HULL Thermo rack Save New KeyBoard Thermo rack Sample no 48 Sample no 48 Check 48 collection tabes on the Thermo Check 48 collection tabes on the Thermo block block Back Next Skip Confirm Sample Barcode 4 Save New KeyBoard Save New KeyBoard Thermo rack Sample no 48 Check 45 collection tubes on the Thermo block Thermo rack Sample no 48 Check 48 collection tubes on the Thermo block Confirm Confirm Once all barcodes are keyed in select save to export a txt documentation file of the 19 barcode then select confirm to continue to the next step To redo the barcode input step select New to refresh the barcode input sheet and key in all barcodes again Check the box in front of keyboard to manually key in sample barcode
27. once and then turn on the machine If this message remains please contact our customer service E e XY ZW T Heaterl Heater2 connect error checking the RS232USB connection This message indicates that the controller does not connect to computer Please turn off the LabTurbo 48 Compact software and the machine After all are turned off switch the main power off and on once and then turn on the machine again If this message remains please contact our customer service ah Kit has been stored over one year This message could show up during the startup of the LabTurbo program if the current extraction kit is expired The extraction kit can be stored for one year and expiration date is shown on the top of extraction kit Please replace the kit with a new one and scan the barcode on the new extraction kit under the Barcode page 41 LabTurbo_48 Compact ss Barcode Reading LabTurbo La DNA Input the Bottle Barcode Mini Kit 480 500 ul KeyBoard Cat No LGD480 500 DLL LTL CCEB Store at 15 25 C ETOH Lu PR Lot No 48074011110 E Date 2012 11 dCCEB ve ue oe 11152013 11152013 1115201535 11152013 11152013 1024 2013 j 485 6263444 487020031201 483335111101 482111011203 4811362604 486 not m use II ALU kit Lot No 48074011412 Exp Date MM YYYY 12 2015 New Save Back XXX buffer has been stored over three months This message could show up during the startup of the LabTurbo pr
28. r extraction protocol Save in LGD3 In500 E100 V 7 0EF fff HV LGD3 In500 E100 V7 0E pro HV LGD3 1n500 E100 V7 0E pro Jc XP Embe Sye Hy Documents and Seinge e Ad n nast ates HV LVNI1 In600 E60 V6 9E pro Sampling SD 220ul pro test HV LGD3 InS00 E 1 00 V 7 0E pro test WS LGDI In220 E100 V 7 4F pro WS LGD1 In220 E100 V7 4E pro XNN LGD3 In300 E100 V 7 0E pro vvuh LGD3 In500 E100 V7 0E pro Kit Lot No 48074011412 Exp Date MM YYYY 12 2015 Save Back 14 6 2 Sampling 1 For sampling from primary tubes or 2 0 ml screw tubes select Sampling Menu or er ce Barcode Sampling 82 3 Extraction PCR setup O Turn off 2 Select Sensor Detection for using ultrasonic to detect the liquid level of blood Clean primary tubes customization and aspirate the sample with top down motions Select Equal Level for using tip senor to detect the bottom of 2 ml screw tubes and aspirate the sample from the bottom of each tube User name or adapter name can be keyed in by using keyboard or barcode scanning LabTurbo_48 Compact Sampling Protocol Sensor Detection Equal Level Barcode Digit 12 9 Next d Rack 3 Put primary tube rack with sample tubes in the machine and select the input sample number and protocol then tap Next LabTurbo_48 Compact Sampling Protocol es Sample No Waste level Syringe status T Safe 7606 Sampling SD 300uL pro
29. s that worktable setup at Vacuum manifold is incorrect Take the worktable setup of 6 samples as an example an additional SC adaptor is misplaced at position 7 left picture Only 6 column sets should be placed on Vacuum manifold right picture represents the sample position at Vacuum manifold or 7 in the aforementioned example Add absent Column on the vacuum manifold This message indicates that worktable setup at Vacuum manifold is incorrect Take the worktable setup for 6 samples as an example a column is missing at position 6 46 left picture 6 column sets should be placed on Vacuum manifold right picture represents the sample position at Vacuum manifold or 6 in the aforementioned example c WE S M GP Am EY Add absent Column SC adaptor on the vacuum manifold This message indicates that worktable setup at Vacuum manifold is incorrect Take the worktable setup of 6 samples as an example a column set is missing at position 6 left picture 6 column sets should be placed on Vacuum manifold right picture represents the sample position at Vacuum manifold or 6 in the aforementioned example Plug set miss install plug set at wash vacuum block correctly This message indicates that the Plugset is not at correct position left picture Put the plug set at correct position on vacuum manifold Please refer to the Monthly clean and maintenance sec
30. sult in damage to the instrument are clearly stated at the appropriate places throughout this manual Warning Notes The following signs are used to warn the users against A warn ING Ignoring this symbol could be potentially lethal NCAUTION Ignoring this symbol may lead to physical injury and or damage to the machine or hardware options Before operating the machine please read and follow the manual in detail 2 1 Normal use J WARNING Improper use of the LabTurbo system may cause personal injuries and or damage to the instrument The use of the machine should be under the instruction of trained technical staff Qualified personnel should follow the manual to operate the device WARNING It is mandatory that suitable protective equipment must be worn at all times when using the machine The machine should be turned off when cleaning repairing or lubricating NCAUTION In case of emergency switch off the LabTurbo 48 Compact power located on the front of the device NCAUTION We strongly recommend the users to use accessories and disposables of LabTurbo 48 Compact Non LabTurbo accessories and disposables may result in damages of the instrument N caution Please do not eat drink smoke apply cosmetics or handle contact lenses near the machine Wash hands thoroughly after handling samples and reagents 2 2 Equipment assembling carrying and positioning requirements caution LabTurbo 48 Compact is
31. then put the same number of empty 6 strip sample tubes to Sample lysis thermoblock 21 9 Place new column sets on the wells of Binding washing vacuum manifold 10 Insert 6 stripprd elution tubes or 1 5 ml eppendorf 2 0 ml eppendorf one by one into the rack of Elution vacuum manifold 11 Once every worktable unit is checked and confirmed ensure sufficient amount of reagents in reagent reservoirs and empty the trash can then click Next Finish Please click Next to continue 13 When the extraction completes the System 22 Message will pop up Open the safety door and take the elution tube out from the Elution vacuum manifold system Message bd i Program completed 14 During the run users can click Pause n or open the safety door to stop the extraction procedure temporarily Click Continue to continue the procedure or click Reset to terminate the procedure LabTurbo Message Device Control Continue yumm On P Reset Elution Off Thermal Control Sample 56 On HCCEBS6 v Off System Pause 23 The following s the general control panel of LabTurbo 48 Compact program abTurbo 48 Compact mI No 3886 n 2 Elapse Remain Total 26 kel i 4 Q kd Q O Current 1 l The Main Toolbar contains several shortcut buttons for convenient operation of the machine 2 The Protocol Window indi
32. tion section 7 3 for the plugset setup instruction 47 Check the Target Bottom This message indicates that the columns sample tubes or elution cover are not set properly Please open the door and press the column sets or the sample tubes all the way to the bottom to set them in right positions Low vacuum pressure switch off the waste bottle and try again Open the door of waste system and switch off the waste bottle a Remove the dropping Column Adaptor and then click Continue This message indicates that SC adaptors fall on the worktable 1 If the worktable is shown as the picture below the robotic arm still holds the column please remove the SC adaptor from worktable and put the columns into the dry spots manually Close the door and click continue 2 If the worktable is shown as the picture below the robotic arm has already transferred the columns to the dry spots Please remove the SC adaptor from worktable and click continue Clean the waste bin Check and clean the Tip disposal vent at worktable and the Trash can Low elu vacuum check all air plug in correct position 1 Check the air plugs of the elution manifold Press down the air plugs to the correct position 2 Close the door and click Yes 3 Ifthis message remains please click No 49 4 I Another message will show up Spin down all column instead of vacuum
33. workstation consist of several parts including a built in touch screen a safety door a power button a side ports four machine feet with wheels a pump system and a waste bottle 3 1 Built in touch screen e OOO Zu The sensitive built in touch screen s on the top front of the machine This control panel equips with Windows user interface Once the panel is turned on the LabTurbo program will automatically activate and users can select the desired protocol to start An included stylus is the accessory of the touch panel 3 2 Safety door The main workstation of LabTurbo 48C has a safety door to form a closed cabinet This protects users from being exposed to any risks or dangers when the machine is up and running It also isolates the worktable from the external environment reducing the possibility of contamination If the safety door is opened while the machine 1s functioning the machine will pause 3 3 Power The red power switch is on the front of the workstation Press the red button to turn on the workstation and directly enter the LabTurbo program To turn off the system exit the LabTurbo program and then press the power button directly 3 4 Automatic reagent feeder Five automatic reagent feeders are located on the inner side of the safety door They are the buffer supply for the buffer tanks on the worktable The buffer from the left to the right is DLL VLL LTL DRVL RLL C
Download Pdf Manuals
Related Search