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1. an k REPLACEMENT PARTS LIST Replacement Parts List Fibrometer 60415 CATALOG NUMBER DESCRIPTION 76000 Fuse Clip and Tie Lug 76001 Fuse Clip and Wire Assembly 76002 Bottom Cover Assembly 76003 Front Cover Assembly 76004 Relay and Switch Assembly 76005 Printed Circuit Board Assembly TAR 76006 MEE Printed Circuit Board Assembly 46 76007 Solenoid Assembly 76008 Motor Assembly 60 cycle 76009 Reed Switch Assembly 76010 Cam Assembly 76011 Bearing Bracket Assembly 76012 Heat Block Assembly 79 SCHEMATIC DIAGRAM FIBROMETER punoig s sseu far pus gt 19013002 1501 poo g 218 IF e 1 ia m 95904 1 6S09 0S yer 1626 EZ 1 v z gz SEN 24904 0609 05 94 a ae A ME el f89023 403 1 6609 0S 019 r e V aps e 2890 rooi90S 90202 8219 09 229 cM 8l a 8 ewk SH O Luo y AINZVO 90202 8219 08 Or 4 7 Z f ELEINZ O v gt v 9 E T7 Z puno o s sseuo E SI 91904 18209 08 7 r az0905 pug AO0SPj O0S i j z AOS PITOS 12 09404 1 1619 0S Y lt 092402 1 161909 6SO0SNI 199 6S0SNI 299 8904 1 9609 0S EH 84904 1 9609 0S zu 90404 g 1 Z 19d pue Z ZHd uee j8g ily ur 23 O6ZE6 229 08 9S80Z 1 218 05 SG404 9819 05
2. Directions for Use for the FibroSystem The Total System for Coagulation Testing l ibrometer Precision Coagulation Timer Catalog Number 60415 NHRIC Ref No 8292 213413 Thermal Prep Block i Catalog Number 60419 NHRIC Ref No H 8292 213513 tonm tie Electric Pipette Catalog Number 60420 NHRIC Ref No H 8292 293613 TRADEMARKS The following are trademarks of Becton Dickinson and Company BBL BART Fibrometer FibroPlastin FibroSystem FibroTip FibroTrol FibroTube and B D ABBE division of Becton Dickinson and Company Cockeysville MD 21030 USA ee lS p E MA PRU ON aT PR E First Edition Copyright 1976 by Becton 7 7 Dickinson and Company Alt Rights Reserved Second Edition Copyright 1977 by Becton Dickinson and company All Rights Reserveds n Z u Preca tiornis e e XC I c co Section 1 FibroSystem The Total System for Coagulation Testing Section 2 The Fibrometer Precision Coagulation Timer Section 3 The Automatic Pipette Section 4 The Thermal Prep Block Section 5 Standard Operating Procedure Prothrombin Time Test section 6 Preventive Maintenance Procedure for 60420 amp 60422 Automatic Pipettes Section 7 Use of FibroTube
3. Hand align the Electrodes so that they are parallel to Probe Body and reasonably straight Check Volume in Cup Insufficient Sufficient Volume Volume SEE MANUAL SECTION SEVEN Helpful Hints Check Cam 76010 SEE REPAIR PROCEDURE 6 SEE REPAIR Call BBL Hot Line PROCEDURE 6 X R We 68 FLOWCHART NUMBER 6 t PROBLEM Short Timing or Machine Stops Immediately or 0 5 Sec after Probe Drop Fibrometer Check Probe for Hanging Materials Materials Hanging From Probe Electrodes Clean Wipe Electrodes Clean with lint free lab grade tissue i n Check Reaction Wel 7o vers Reaction Well Clean Accumulated Dirt in Reaction Well Clean with lint free tissue moistened with Isopropyl Alcohol Mi Check Electrode Length SEE REPAIR PROCEDURE 7 Electrode Length Electrode Length Needs Adjustment Is Correct U SEE RERAIR Call BBL Hot Line PROCEDURE 7 69 Deeg TROUBLE SHOOTING FIBROMETER ADDITIONAL PROBLEMS Listed below are problems that may occur with the Fibrometer but have not been covered in the flowcharts PROBLEM SOLUTION 1 Probe will not remain in the UP e 1 See Repair Procedure 1 Position Probable Cause Bent probe support lever 2 Errat
4. It is a good practice to touch off the tip against the side wall of the liquid reservoir to insure that droplets do not cling to the exterior of the tip AE Y i d i Dispense the liquid into a FibroTube by completely depressing the pluhger The FibroTip Should be inserted 1 4 of the way into the FibroTube Cup while gesting i against the lip closest to the pipette as shown below in Fig 3 6 After ejecting the liquid be sure to withdraw the pipette tip before releasing the plunger 27 i NOTE DO NOT let the FibroTip touch the opposite side wall of the FibroTube Cup An unequal liquid level will result if the liquid is allowed to run down the side wall This will yield an erroneous end point Figure 3 6 Testing With The Fibrometer The previous instructions apply to pipetting when the user does not wish to start the Fibro meter action To activate the Fibrometer flip the Pipette switch to ON before dispensing the final aliquot of liquid CaCl plasma etc Electrical contact is made at the end of the plunger stroke i e the instant the final bit of liquid is dispensed Assuming the Fibrometer has been tested independently the following is a guide for Pro thrombin Time Test protocol in which the automatic action of the Pipette may be tested 1 Begin by preparing the Fibrometer for the Prothrombin Time Test Plug the Fibrometer into a standard grounded three prong
5. PRECISION COAGULATION TIMER T HELPFUL HINTS The FibroTube Cup is an integral part of the Fibrometer because it precisely and consistently presents the sample to the Fibrometer probe The FibroTube disposable plastic cup is pro tected under U S Patent 3 219 421 The Patent was granted based on the dimensions of the FibroTube cup which enables the cup to perform its precise function in the Fibrometer The internal diameter 1 0 of the FibroTube cupfixes the liquid level at a precise height when a total volume of 0 3 ml is placed within the cup The spherical radius of the bottom of the FibroTube cup exactly matches the spherical radius of the tube well of the Fibrometer This fact in concert with the uniform wall thickness of the FibroTube cup provides heat transfer from the heating block to the liquid within the cup i 4 i Figure 7 1 shows a cutaway view of the heat block probe assembly and associated BEBE clot detection apparatus In this figure clot has been MOVING detected and the Fibro ELECTRODE meter has stopped STATIONARY The moving electrode has HEAT BLOCK ELECTRODE mechanically caught a fibrin strand during its sweep through the material in the cup and CUR then lifted to a point 1 27 mm above the level of the liquid i LOT in th cup LIQUID LEVEL At this time and only at this FOE b r mr FOOT 1 time the moving electrode is T electricall
6. 17 aperdacay ajeW E puno1c OVA SECTION NINE WARRANTY AND REPAIR POLICY 87 Repair Policy Fibrometer 9 and Thermal Prep Block 1 Any machine that cannot be repaired as directed by the Trouble Shooting Sec tion Section Eight should be returned to BBL for repair A loaner machine is usually available for immediate shipment on request Call the toll free number 800 638 8663 to arrange for shipment of a loaner machine and repair of your machine BBL will allow one month from the time the machine is shipped until receipt of the customer s unit for repair BBL will also allow one month after shipment of the repaired unit for return of the loaner Failure to meet these dates may result in a delay in shipping a loaner to you when you need it These loaner Fibrometer Timers and Thermal Prep Blocks are not for sale 2 Please do not ship your unit to BBL until the loaner is received provided of course that a loaner was necessary Call Technical Services at BBL 800 638 8663 to determine if a loaner is needed The loaner unit will have with it a defective unit return authorization and a peel stick BBL address label Please check off the list of possible defects any which you may have experienced Add any note you feel may be helpful in repairing the unit Pack your Fibrometer in the loaner box with the defective unit return authorization Seal the box and place the BBL address label on it 3 Do not ship by Parcel Post unless yo
7. O Ring B Improper seated or defective tip 4 Inaccurate delivery Probable Causes A Improper techique B Defective O Ring 71 SOLUTION 1 See Flowchart 2 2a See Section VI 2b See Section VI INIM we 3a See Section VI 3b Firmly secure the tip into Pipette Check the FibroTip for obvious flaws 4a See pipetting directions Section III 4b See Section VI Teea NT wm wot bobus m REPAIR PROCEDURES NOTE READ WARRANTY BEFORE BEGINNING ANY REPAIRS ONLY TRAINED PERSONNEL SHOULD ATTEMPT INTERNAL REPAIRS WARNING OBSERVE ELECTRICAL SAFETY GUIDELINES Fibrometer and Thermal Prep Block LJ 1 Probe fails to drop or it will not remain in the UP position A Check to see if the machine has a faulty time delay circuit or solenoid 1 Remove the probe 2 Activate the machine i 3 Watch for the probe support lever brass lever on the right hand side of the probe hole to snap inside the sleeve out of sight P 4 If the lever does not retract the machine has a bad time delay circuit or solenoid Factory repair will be done by BBL If desired a faulty solenoid may be replaced with a new solenoid assembly BBL Catalog Number 76007 The time delay capacitor if found defective may be replaced BBL Catalog Number 70615 B If the lever does retract and the probe sleeve has been checked for dirt accumulation the lever must be out of alignment The lever arm shou
8. When machines are in a modular arrangement keep them on a level surface and be sure to avoid twisting the connectors j j f Cups FibroTube cups single use disposables manufactured by BBL The liquid level in the re action well is critical to the operation of the Fibrometer i i Reagent Quality E j pi FibroSystem Reagents are recommended for the best test results However any properly pro duced and correctly s standardized reagent and control pn will work with the Fibrometer 1 Reagent Volume DECEM The Fibrometer comes with a obs calibrated with exactly 0 3 ml of li uid A 0 4 ml probe i is available for use in tests requiring this volume To ensure proper liquid levels it is recommended that FibroTube cups be utilized for all tests To ensure correct volume de livery use the Automatic Pipette BBL Catalog No 60420 i Temperature Warnings Tbromboplastin reagents Should never Be lek at 37 2 fori more than four hours Pl sma should be left at this temperature for more than twenty minutes H owe UU we ii aan MAINTENANCE The Fibrometer is designed with user convenience in mind There is a minimum of mainten ance required The timing mechanism is factory sealed and never requires lubrication There are no zero calibrations threshold settings temperature controls or day to day adjustments necessary with the Fibrometer All that is
9. Z 2d JQAMOSI Pil 0 v2 289012 H2S UO vae Uwe m 21 z E ouros 2 09404 1619 06 ELL0L 9209 09 Pos 26904 1119 09 Prouejos cud 01 VEMS ZIH 8486NI 5 a 22102 P4 LOL 029 09 ps ly 42 85 Heater Circuits mE Motor Timer and Circuit 5 1 ES A Indicating Circuits i CAIS y IVA Cit MCN 9190 9209 08 eAug 31un03 20AMO001 17l i gutig 9q iN 0 pz90 99 380 9 09 0S 2 2111 Vz Vz ATS z o dA1 00404 1 1Z19 0S 49 09404 1 1619 0S p99tJ S 6SOSNI 30 SZ 04 0919 09 Uo o _ Alv e 5a quvos 124 64904 1 2609 0S H _ zz 28 i zz 6S0SNI iz xes ai z 904 44904 1 S609 0S S8S0 1 SZ19 0S 22 1619 0S nemi 9 H yu 1 84 apoq eo Nepoue 91 99100 9819 05 995 10 zi isison 0 E1902 9 Ln 17 98504 1 609 0S 1 SZ09 0S IGAMOOS P37 Z00 9 2689 781 8909 09 191889H e 27 Z 7 s h 2 81 41014 S0904 dz 1 6129 08 I 109 0S 001 591 10813 uoeN 3e15 1 noa 9N sq zs 2219 08 _ 3 EM IS 0 v1 punoas s sseu3 uo 4 a 62902 V 2909 09 esnj vi 14 zz Ezr 14904 02902 6809 06 Ear 8809 06 it ajeuia 4 gt V
10. i Fibrometer i in the XEWE Plug the Pipette cord securely into the front jack of the Fibrometer Activate Machine Ddes Not FLOWCHART NUMB PROBLEM Probe Fails to Drop Fibre Check Power No Power 2 HEC UNES SEE FLOWCHART NUMBER 1 Check Probe Sleeve for Dirt Remove all accumulated dirt with lintless tissue moistened with 7096 ISOPROPYL ALCOHOL Clean groove at rear of probe and plastic sleeve in probe hole Check Probe support lever Probe support lever Bent Probe support lever Not Bent rm d SEE REPAIR SEE REPAIR PROCEDURE 1A PROCEDURE 1B 64 FLOWCHART NUMBER 4A PROBLEM Overheating More Than 37 7 C Fibrometer 9 Check Machine Location H Machine Is On Same Bench with Centrifuge Machine Is Not On Same Bench with Centrifuge Move one of these instruments to different bench or insulate with Isomode Pad i i 1 SEE REPAIR qm PROCEDURE4 Thermostat j 70585 SEE REPAIR PROCEDURE 3 i Thermostat Is Faulty Thermostat Is Not Faulty Replace Thermostat Pa 70585 70609 or Call BBL Hot Line return to BBL for EM repair n 1 bc 4 2 5 D 4 NOTE lf the heating system is faulty the warming block will usually be too h
11. 40821 into each of the four remaining FibroTube NOTE The use of FibroPlastin Reagent is recor mended This is the reagent used by BBL to calibrate all Fibrometer Tim ers at the factory 18 7 Allow three minutes for room temperature liquids to come to operating temperature in the warming wells Five minutes will be necessary for refrigerated liquids 8 Place one FibroTube cip with FibroPlastin into the center reaction well 9 Pipette 0 1 ml of FibroTrol Con trol Plasma into the cup and sim ultaneously press the Timer Bar NOTE With the Automatic Pipette there is no need to press the Timer Bar When the Auto matic Pipette switch is ON the Fibrometer action is begun automatically as the last B of liquid is expelled from the pipette 10 The probe should drop and the moving electrode should begin its elliptical sweeping pattern through the reaction mixture A clicking sound will be heard This is normal 11 The digital readout and electrode action will stop when a fibrin strand is detected Read and record the time required for fibrin strand detection If the Fibrometer has properly detected the formation of the first fibrin strand the time shown on the digital readout should be from eleven to thirteen seconds gt M 19 RT A oo helay an agn yy a 12 Lift the probe arm up to the rest position The electrode should be wiped dry with a lint
12. Catalog No 60416 These times are supported by data obtained using the Quick one stage technique and the Ware Stragnell modification of Owren s test for Prothrombin and Proconvertin 3 4 Ue LU Sie Iti emerge Mein 6 ace wen am PCIe E nea Rain p ra P dt PF ere pem qo 7 rer orm y COMPONENT PARTS The Probe Unit The clot detection arm of the Fibrometer is the probe unit Two probe units are available and may be used interchangeably on any Fibrometer Each Fibrometer comes with one standard probe BBL Catalog No 60417 This probe is designed to be used with samples having a volume of 0 3 ml For samples that require 0 4 ml the user may order a special probe BBL Catalog No 60418 The probe unit is made of a cylindrical body two sensory electrodes and a heavy wire called the probe foot which extends from the base of the assembly These structures may be seen in Figure 2 1 i ame 2 n h 4 MOVING ger nc ape GOTT ETE Y oe ELECTRODE he STATIONARY ELEGTRODE 4 A The entire probe unit fits smoothly into the probe sleeve with the electrodes positioned directly over the reaction well Figure 2 2 PRES SE IY x ve iC kak ZE Gi a DAS SM NESTOR ROO AEN Sk CC PEREZ TEWTEWE DD TRUE CUI n IAE C ORT LSE Reto EBR Ro o Between tests the probe
13. FibroSystem 9 duidimpiit in the Pro thrombin Time Test It is recommended that beginning personnel and new users of the Fibro System work their way through Sections One through Four to independently test equipment and learn procedures 1 Connect one Fibrometer and one Thermal Prep Block in modular sequence Place the Fibrometer to the left of the Prep Block Carefully connect the three pront ed receptacle on the right side of the Fibrometer to the plag on the left side of the di lock Connect the line cord to the three pronged plug on the left side of the Fibrometer Be certain to match the alignment dots found on both the line cord and the Fibrometer plug 3 Plug the line cord into a standard laboratory grounded three wire outlet 4 Switch ON the Fibrometer and the Prep Block Both machines should be at operating 5 Lift the Fibrometer probe arm to the UP position dispense into a FibroTube Cup labeled Control i i temperature in approximately fifteen minutes 2E Plug the Automatic Pipette cord into the lower left front receptacle of the Fibrometr Leave the Pipette switch in the OFF position Prepare the FibroTrol test solution You will need 0 1 ml for each test Assume that you will run TWO tests Using the Automatic Pipette take up 0 2 ml of FibroTrol and i I Place this cup into a plastic rack and set over one of the shallow wells in the Prep Block Do this after the Prep Blo
14. Length 184 7 25 Net Weight 2 9 kg 6 45 Ib Shipping Weight 4 kg 9 0 Ib Block Warm Up Time Approximately fifteen minutes Liquid Warm Up Time Refrigerated liquids five minutes Room temperature liquids three minutes Electrical Specifications 117 volts 50 or 60 Hz 0 6 ampere i d i COMPONENT PART PRINCIPLES OF OPERATION Heating System A heating circuit regulated by a precise mercury thermostat holds the warming bjock at 37 2 0 5C Heating Block An epoxy coated durable aluminum block provides precision heat transmission tQ liqu ids anywhere i in the block f Indicator Lamp When the unit is plugged in and the switch is in the ON position the neon indicator light will come on immediately The indicator light will remain on to indicate the heating circuit is functioning When the warming block reaches a temperature of 37 2 0 5 C the thermostat will switch out and the light will go off When the thermostat calls for heat the indicatpr light will come back on NOTE The action of the Thermal Prep Block indicator lamp is not the same as the Fibro meter indicator lamp In the Fibrometer the indicator lamp is designed to glow when liquids reach the required temperature Remember d 1 Fibrometer machine ON light ON liquids ready for use i xi 2 Thermal Prep Block machine ON light OFF liquids ready for use i 37 Power Supply The
15. Pipette is constructed of a light weight chemical resistant plastic All mechan ical parts of the pipette are enclosed by this protective plastic The liquid aliquot is held in and dispensed from a FibroTip Disposable Tip BBL Catalog No 70314 This special disposable tip seated in the gun has been designed to prevent liquid from touching the pipet ting apparatus Only the tip a disposable item touches any of the samples or reagents used in coagulation studies This keeps cleaning and maintenance of the Automatic Pipette to a min imum The cleaning procedures are to be performed after every two months of use 0 2 CALIBRATION 000000 PLUNGER ALIGNMENT INDICATOR i 0 1 j H i ON OFF CALIBRATION SWITCH FiBROTIP TM Figure 3 1 The Automatic Ripette 25 The Automatic Pipette is under warranty for ten years or until the product is no longer a part of the BBL line whichever is the longest This warranty covers defects in materials and work manship The warranty dees not cover abuse Replacement units can be obtained by calling the Technica Services Department 800 638 8663 toll free in Maryland call 301 666 0100 collect OPERATING INSTRUCTIONS The Automatic Pipette is supplied ready for use It comes complete with an electric cord and a supply of 200 FibroTip Disposable Coagulation Tips Independent Testing The following seven steps are designed to test the Automatic Pipette without activating
16. enables the counting system to have a resolution of 0 1 second t Readout Reset Button This small red button resets the counter to zero when depressed firmly for at least one second Indicator Lamp The function of the indicator lamp is to let the user know when the machine has reached op erating temperature This light will come ON only when operating temperature has been ob tained It will stay ON as long as the machine remains at this temperature i DO NOT perform Fibrometer tests unless the indicator light is ON Safety The Fibrometer is certified for safety by the Los Angeles County Testing Laboratory an n is listed by the Canadian Standards Association CSA n i COMPONENT PART PRINCIPLES OF OPERATION 4 j f This section should be read and understood completely before beginning routine use of the Fibrometer The lector Cutie and avian below are vital to accuracy d Heating System EO i The heating block contains two mercury thermostats and a heater that will bring liquids in the wells to 37 2 0 5 C The operation of these thermostats is automatic The temperatute can not be adjusted by the operator This unit will bring liquids at room temperature to approximately 35 C in about ten minutes At this pojnt the indicator light comes and the control thermostat takes over This sensitive thermostat will react to a 0 1 C temperature change inside the block and
17. final check by run ning a normal control and an abnormal control to be sure the correct end points are de tected Figure 8 1 74 be t B Adjustments for no end point or for considerably longer than correct end point 1 To lengthen the electrode re move the probe and hold it in your left hand with the black cap to the ceiling and the electrodes facing you 2 Place your right forefinger on top of the right angle formed by the moving electrode See Figure 8 2 3 Place your right thumb on the bottom of the probe foot and apply approximately 1 0 Kg of upward pressure This should lengthen the moving electrode 4 Test the electrode length by allow ing the probe to drop into 0 3 ml reagent only If the machine stops perform the shortening procedure outlined above Perform a normal and an abnormal control to be sure that the correct end points are detected If the problem con tinues call the BBL Hot Line Figure 8 2 8 Correct Protimes will not detect PTT s See Procedure 5 9 Long PTT and Long PT Problem is a dirty cam See Procedure 6 11 Digit Repetition On occasion a machine will develop a constant display of one of a pair of digits separated by 0 5 second 0 2 and 0 7 0 3 and 0 8 or 0 4 and 0 9 Even though this state of constant display does not cause results to go beyond the stated accuracy of the machine many users find this condition to be undesirable The problem must be c
18. if a 0 3 ml total volume is placed within a competitive cup whose internal diameter is slightly larger than the BBL FibroTube 9cup then the liquid level will be considerably below the level obtained in the BBL FibroTube cup With such a low liquid level it is possible for the moving electrode to lift the clot above the liquid in the cup breaking the circuit the Fibro meter will not stop Figure 7 3 Both of these problems are best rectified by use of BBL FibroTube cups EU The problem of short times or a no detect condition can also be caused by maladjusted electrodes Short times can be caused by the moving electrode being lengthened by the cleaning procedure over a period of time The normal cleaning procedure after each test is to raise the Ji probe to the rest position and wipe the electrodes down with the lintless tissue Depending upon the length of the time in service and the degree of squeeze exerted upon the electrode it is possible to lengthen the moving electrod by 0 64 or 1 30 mm This condition can be corrected by holding the probe in your left hand with the black cap toward the ceiling and the electrodes facing your right At the very bottom of the cylindrical body you will notice a 3 single wire which comes out straight 6 35 mm then curves this is the probe foot Grasp E the probe foot with your right hand and pull down with a steady force While you are pulling down watch the hook end of the
19. into the 0 2 ml mode Fill the FibroTip and deliver 0 2 ml of Fibro Plastin Reagent BBL Catalog No 40775 or BBL Catalog No 40821 into one of the cups labelled Reagent Repeat this process for each of the three remaining Reagent cups NOTE It is not necessary to change FibroTip Tips as long as you are pipetting the same 13 14 15 16 17 18 19 concentration of a given reagent In Step 12 the same FibroTip can be used to fill dispense the FibroPlastin into each of the four cups 3 Allow three minutes for room temperature liquids to come to operating temperature Five minutes will be necessary for refrigerated liquids Place one FibroTube Cup with 0 2 ml of FibroPlastin into the center reaction well Replace the FibroTip Adjust the Pipette to the 0 1 ml mode With the Pipette switch in the OFF position depress the plunger fully and Sent the tip into the FibroTrol liquid 1 Allow the plunger to retract n to pick up the 0 1 ml aliquot _ n Move the Pipette switch to ON TP 20 Insert the FibroTip into the Fibro Tube Cup containing 0 2 ml of FibroPlastin Reagent The FibroTip should be 1 4 of the way into the cup and resting on the lip closest to the pipette 21 With the Pipette switch ON dispense the 0 1 m of FibroTrol into the 0 2 ml of FibroPlastin NOTE There will now be a 0 5 to 1 8 second built in delay This will in no way invalidate the test resul
20. maintain a temperature of 37 2 0 5C 13 LJ N Xud m IN FE Mechanical Action Probe Drop The Fibrometer can be activated manually by depressing the TIMER bar or automatically when the Automatic Pipette has delivered its last bit of liquid into the reaction cup There is a built in delay of 0 5 to 1 8 seconds before the probe drops into the reaction well The delay allows for the removal of the pipette tip from the reaction cup This delay will not affect the test results After the built in delay the sensing probe assembly drops into the reaction well Before the testing of a sample the probe is placed in its rest position above the reaction well In this position the probe rests upon the probe support lever This lever is mechanically connected to a solenoid When the delay circuit energizes the solenoid the lever snaps back inside the probe sleeve te deprive the probe of its support and causes it to drop The drop places the electrodes into the proper position inside a FibroTube cup in the reaction well Mechanical Action Electrode Movement Movement of the sweeping electrode is supplied by a segmented cam that rotates at 60 rpm and raises the probe foot every 0 5 second This sweeps the electrode through the reaction mixture every one half second The electrode moves in the desired elliptical pattern through the reaction mixture This can be viewed as a four step process 1 The moving electrode sweeps
21. outlet Switch ON the Fibrometer Wait for the indicator light to come ON approximately 10 minutes Raise probe to the UP position Place six FibroTube cups into the warming wells Label one cup Control Plasma one cup Patient Plasma and the remaining four cups Reagent mooo r Insert a FibroTip into the Pipette Insert the plug on the Pipette cord into the lower left front jack of the Fibrometer 28 mes ee DE a Turn the pipette plunger so that the 0 2 ml calibration notches align with the indicator 5 Depress the plunger and fill the tip with 0 2 ml of FibroTrol Control Plasma BBL 10 11 12 Catalog No 40777 BBL Catalog No 40778 Remember depress the plunger fully before inserting tip into the reagent and then allow the plunger to retract fully to be certain of picking up the correct volume Deliver 0 2 ml of FibroTrol into the FibroTube Cup labelled Control Plasma Reset the pipette to the 0 1 ml mode Fill FibroTip with 0 1 ml of FibroTrol Deliver 0 1 ml of FibroTrol to the FibroTube Cup labelled Control Plasma pontaining 0 2 ml of the reagent This cup will now contain 0 3 of FibroTrol reagent The same procedures be done with patient plasma if desired Be sure to REPLACE the FibroTip with a new tip before going from FibroTrol to patient plasma 4 Replace FibroTip with a new tip Set the Pipette
22. required of the user are a few simple procedures as outlined below Electrode Care Protein build up can significantly alter test times Remove all dust debris and testing contam ination from the electrodes Clean electrodes of foreign material by wiping the electrodes with lint free tissue or paper or rinse with distilled water by using a wash bottle Warming and Reaction Wells Periodically remove accumulated dust and debris with a slightly dampened cotton swab Dirt or debris will raise the level of the FibroTube cup This in turn will raise the level of the liquid in the reaction well and drastically alter the coagulation time measurements Check the well each day before use Probe Column No portion of the probe assembly should ever be lubricated The materials and mechanism of this probe are designed to operate dry After approximately 1 000 probe drops the post hole and the probe column must be wiped with a lintless soft tissue This should be done even if no dirt accumulation is visible Should dirt be allowed to accumulate in the post hole it must be wiped clean with a tissue moistened 70 isopropyl alcohol DO NOT use any other solvent Top Surfaces and Case Use a dry or slightly dampened cloth to remove dust water or plasma stains This should be done daily Remember that all blood plasma and serum is a potential hepatitis hazard Internal Mechanism The internal mechanism should not be touched except in specifi
23. through the reaction mixture 2 The electrode lifts completely out of the reaction mixture to a point 1 27 mm above the liquid level NOTE This is why the FibroTube cup is of such importarice in the FibroSystem Use of these cups ensures proper liquid level 3 The electrode becomes electrically active 4 The electrode is returned to the liquid level and sweeps through the reaction mixture to complete an elliptical pattern Clot Detection As the initial fibrin strand forms in the reaction mixture it is picked up on the next sweep of the moving electrode see F igure 2 3 When this electrode is lifted to a point 1 27 mm above the level of the liquid in the FibroTube cup a portion of the fibrin strand will remain in the liquid At this point there is a stationary electrode remaining in the liquid reaction mixture moving electrode situated 1 27 mm above the liquid level and a fibrin strand connecting the moving electrode to the reaction mixture When the moving electrode is lifted to its position above the liquid level it becomes elec trically active A fibrin strand will complete the circuit Current flows from the stationary electrode through the liquid and through the fibrin strand to the moving electrode The circuit is completed as current flows from the moving electrode to the cam and detection circuit The completion of this circuit causes the Fibrometer and its timing device to stop This is vhy the Fibrometer is known as
24. time it would be wise to decide if additional space is needed for other modules of the Fibro System We CAUTION DO NOT place the Fibrometer on the same bench with a rotary centrifuge or shaker Mechanical shaking may separate the mercury column in the sensitive mercury thermostat and impair the ability of the unit to hold the correct operating temperature Should such a problem occur see the Section titled Trouble Shooting 3 Plug the grounded three wire line Cord onto the prongs found on the left side of the Fibrometer NEVER plug the line cord into the wall receptacle first and then attempt to connect to the machine Insure proper alignment by matching the red dots found above the prongs on the Fibrometer and on the line cord 4 Plug the line cord into a standard laboratory three wire grounded receptacle DO NOT use any other receptacle or modify the line cord to fit any other type of receptacle WARNING AN UNGROUNDED RECEPTACLE CAN CONSTITUTE A SOGIS HAZARD FOR THE OPERATOR Remember to reduce the variables in the testing of all equipment At this point test only one Fibrometer After completion of this test proceed to test additional Fibrometer Timers Thermal Prep Blocks and Automatic Pipettes i Operating Instructions The following instructions are for a test run of the Fibrometer The test being utilized is the Prothrombin Time Test Be sure to run this test several times to be certain the Fibrom
25. utilizing the modular concept a single operator can significantly increase ease of opera tion and work output The following set up is suggested 1 Begin with two Fibrometer Timers and one Thermal Prep Block 2 Connect a Fibrometer to each side of the Prep Block The removable plastic trays of the Prep Block can be pre filled with reagents and refrigerated 4 The instruments can be broui to operating temperature and the tray of reagents can be placed over the shallow wells 5 The reagents can be brought to the testing temperature in five minutes During this time additional trays can be readied and refrigerated 6 Continuous testing and reagent warm up can now begin with no risk of deterioration due to prolonged heating of reagents ZU uenea DOM a Mum imu my k 2 D 35 SHALLOW WELLS _ For FibroTube Disposable Cups DEEP WELLS EN AGA gt j SS 2 Gy S Lee N amp N N 0 3 OX THERMAL PREP BLOCK SELF STACKING PLASTIC TRAYS FOR FIBROTUBE AUTOMATIC PIPETTE DISPOSABLE CUPS Figure 4 1 The Complete System 36 THERMAL PREP BLOCK Performance Characteristics and Specifications i 9 Temperature Range 37 2 0 5C Volumes Up to 0 5 ml in FibroTube 9 Cups in twenty shallow wells Up to 3 0 ml in 12 x 75 mm test tubes in ten wells i Dimensions and Weights Height 102 mm 4 i Width 159 mm 6 25
26. wall receptacle At the same time line power will pass on to the second machine now plugged into the first If a third machine were to be plugged into the second it too would receive power This modular concept will not overload a circuit because of the small amount of electricity actually being used by each machine For example a system composed of two Fibrometer T imers and two Thermal Prep Blocks would use a maximum current of 3 0 amperes The modular concept is expanded still further by plugging the Automatic Pipette cord di rectly into the connector on the front of the Fibrometer Examples of modular combinations are shown in Figure 1 1 SECTION TWO PRECISION COAGULATION TIMER THE FIBROMETER THE FIBROMETER PRECISION COAGULATION TIMER USE The Fibrometer Precision Coagulation Timer is an electro mechanical instrument that will determine the presence of a fibrin clot The Fibrometer is both a clot sensing device and a timer Each unit also contains constant temperature warming and reaction wells This basic design allows the Fibrometer to be used as the primary piece of equipment in most of the coagulation determinations now in common use n 3 PRINCIPLES OF OPERATION Historically those wishing to perform coagulation determinations utilized the wire loop manual method for measuring the critical clotting end point The Fibrometer was designed to mechanically replicate this proven manual method 1 2 Now afte
27. 2 Fibrometer or Procedure 3 Prep Block 5 The Fibrometer does not stop after a clot has formed does not detect an end point A Dispense 0 5 ml of reagent into a FibroTube Cup and place the cup into the reaction well B Start the Fibrometer and allow the probe to drop C Due to the excess of liquid in the FibroTube Cup the Fibrometer should stop imme diately or within 0 5 second after the probe has dropped D Check the electrodes for misalignment They should be parallel to the body and reasonably straight Hand alignment is all that should be necessary E If in step the Fibrometer does not stop check the cam for dirt accumulation and check for dirty electrodes 1 Clean electrodes with distilled water Dry with lintless paper 2 To clean the cam see Procedure 6 below i 6 Cleaning the Fibrometer Cam Assembly A Remove probe B Moisten a well compacted ab with 70 aicanai and lower ihe ond through the probe hole until it rests on the top cam i C Activate he Fibrometer and allow the cam to rotate Move the swab back and forth to clean the solid portion of the cam Repeat with fresh swabs moistened with aono until all dirt is remaved Test with a Prothrombin Time Test 73 D If the cam is found to be defective send the Fibrometer to BBL for factory repair or replace with BBL Catalog Number 76010 Cam Assembly E If step C fails an the cam appears satis
28. Disposable Plastic Coagulation Cups with the Fibrometer Precision Coagulation Section 8 Trouble Shooting and Repair Trouble Shooting a a a cas emo Ab di N Rep ir Procedures 4 344 933 chase eee SoS E Replacement Parts Lists Wiring List Wiring Diagram Schematic Diagram Fibrometer8 Section 9 Warranty and Repair Policy Bibliography DIR CONTENTS PRECAUTIONS Read this manual before testing controls or clinical samples with FibroSystem equipment This manual has been designed utilizing a step by step format It is meant to provide all the information that is needed to run accurate tests for fibrin formation Not only will this man ual serve as a handy reference but also as an important training guide for new personnel FOR YOUR PROTECTION the Fibrometer9 Precision Coagulation Timer and the Thermal Prep Block are equipped with a three wire grounding plug Used properly this grounding plug will protect you from shock hazards Should a two prong receptacle be encountered it must be replaced with a properly grounded three wire receptacle in accordance with the National Electrical Code and local codes and ordinances This wo
29. Fibrometer reaction well E d ANNIS AC RR ue qo DEV AER RID NOTE There will be a Built in 0 5 to 1 8 second delay This will allow you to remove the FibroTip from the FibroTube cup This delay does not change the test results 17 The probe arm of the Fibrometer will drop automatically and begin the sweeping and timing actions 18 First fibrin strand detection should occur between eleven and thirteen seconds The A Fibrometer will stop timing and electrode activity automatically EF 19 Record the time and reset the timer by depressing the red reset button for at least one full second then lift and clean the probe arm 20 Replace the FibroTip and discard the FibroTube Cup from the reaction well of the E Fibrometer E 21 Repeat the above procedures Steps 11 20 to verify the results WARNING Be certain to carefully dispose of all tips and cups that contact patient serum plasma or whole blood There is a HEPATITIS DANGER when working with these specimens 2 NOTE Be certain to change the FibroTip for each change of liquid or concentration being pipetted For example DO NOT use the same tip for both FibroTrol and FibroPlastin 46 07 SECTION SIX PREVENTIVE MAINTENANCE PROCEDURE FOR 60420 amp 60422 AUTOMATIC PIPETTES an HELPFUL HINTS i The procedure outlined in this section is designed to prevent ti
30. N The same pip pipetting each aliquot of a reagent or sample A different pipette for each plasma specimen 20 A AM ves OPERATING HINTS AND PRECAUTIONS The Fibrometer Precision Coagylation Timer is designed for In Vitro Diagnostic Use all directions should be read and followed carefully j WARNING Every humanblood sample represents a potential HEPATITIS DANGER Observe proper precautions against this infectious hazard whenever handling plasma serum or whole blood specimens 5 i i Electrodes N j The stationary and moving electrodes should be aligned in a vertical position pase tojone another Even though spacing is not critical gross distortions should be corrected 1f the electrodes become bent carefully realign them so that they are both vertical and parallel to one another As noted lint contamination of the electrodes will result in erroneous short coagulation times Use lint free laboratory grade absorbent tissue or bibulous paper Mechanical Operation The Timer bar must be pressed firmly to ensure proper probe activation The timer reset button must be depressed firmly for at least one second to reset the digital mechanism Failure to do so may cause slipping or jumping of the timer wheels Wires and Outlets j Separate modular units with care Electrical safety procedures always recommend that the iid cord be unplugged before connecting or disconnecting modular units i
31. T nT FOR ALTERNATE OPERATION WITH WITH AUTOMATIC PIPETTE to ow ELI Figure 2 2 The Fibrometer ue pur O Heating Block 2 gt 22 r A LT i x 7 The chrome plated heating block is designed to heat and hold liquids 37 2 0 5 C The block itseil is composed of metal which has a long thermal constant to ensure that the proper testing temperature will be held Six prewarming wells and a reaction well are built into the block e The Detection Circuit The ineton of the detection circuits to sense the circuit formed completed when a fiprin is detected by the moving electrode When a fibrin strand is detected the Fibrometer _ will stop timing and cease mechanical action 1f no strand of fibrin is present there is no How of current through the detection circuit However when a fibrin strand is detected current flows through the detection circuit and de energizes a relay The relay contacts open and stop both the timing and the mechanical operation The detection circuit may be classified as a bistable or flip flop transistorized circuit The Motor and Counting Unit j The Fibrometer contains a synchronous clock motor to run the counting unit and to produce electrode movement The precise speed of the this motor is regulated by the frequency of the power line The motor has two shafts One shaft moves the cam at 60 rpm The other shaft drives a pulsed counting unit at 600 rpm This arrangement
32. Thermal Prep Block rpay be operated independently by attaching the unit by a three wire line cord not supplied to any approved 110 volt three wire grounded receptacle Most users connect the Thermal Prep Block directly to the Fibrometer Both of these machines have been designed to operate in a modular arrangement A single power source will operate both machines The second machine in line will receive power even when the first machine is switched off e Safety The Thermal Prep Block is certified for safety by the Los Angeles County Testing Laboratories and is listed by the Canadian Standards Association CSA DO NOT separate or connect the Thermal Prep Block to the Fibrometer while the line cord is connected to the power receptacle A Shock Hazard exists when doing so INSTALLATION Equipment Model and Power Rating BBL Catalog No 60419 Thermal Prep Block 117 volts 50 or 60 Hz 0 6 amps Laboratory Requirements The Thermal Prep Block operates on a standard 117 volt A C power source which must meet the National Electrical Manufactures Association standard of 99 5 to 128 7 volts The Outlet must be a standard laboratory three wire grounded receptacle wall or through the Fibro meter Other power sources or outlets may damage the machine The working environment should be as clean and dust free as possible with an ambient temperature between 18 32 C 64 4 to 89 6 F Procedures The following procedure is designed to all
33. a FibroTube Cup and place the cup into the reaction well 72 M 2 Place a small mercury thermometer in the cup preferably a rectal thermometer for babies 3 Allow five minutes for stabilization then read the temperature The reading should be 98 6 1 8 F 37 2 0 5 C NOTE A thermistor type thermometer or a large glass thermometer cannot be used They will conduct heat too rapidly from the small mass of liquid and yield a false low reading C A faulty thermostat can be replaced by BBL or you may order a new thermostat BBL Catalog Number 70585 and the required phefolic sleeve BBL Catalog Number 70609 3 Measuring the Heat Block temperature Thermal Prep Block r Follow the steps in Procedure 2 Use a shallow well of the Prep Block Note The center well i in the second row of deep wells will be slightly warmer than the others If you wish to order a new thermostat for the Prep Block order BBL Catalog Number 76018 If the thermostat is not faulty and tests show a new heat pad is needed order BBL Catalog Number 76015 4 Extreme overheating Fibrometer or Prep Block A Make sure these instruments are not on the same bench with a centrifuge If this is not possible insert TWO layers of Isomode pad available from most scientific supply houses under the FibroSystem 9 instruments Let the machines cool to room temperature and attempt operation the next day B Check thermostats as described in Procedure
34. a fibrin switch detection device Note once again the level of the liquid in the reaction well is critical FibroTube cups en sure that the proper liquid level can be maintained 14 B MOVING i B ELECTRODE B STATIONARY Pn UP POSITION ELECTRODE A B 1 MOVING i ELECTRODE i 4 DOWN FIBROTUBE POSITION z CUP IN E i REACTION WELL i FLUID i E LEVEL zu PATHOF B a MOVING ELECTRODE f E B j i B a P 1 27 mm B B gd ENS UM e 6 0 TY 0 d Figure 2 3 Clot Detection INSTALLATION Equipment Models and Power Ratings d BBL 604156 Fibrometer 117 volts 60 Hz 0 6 amps BBL 60416 Fibrometer 117volts 50 Hz 0 6 amps i A dropping transformer BBL Catalog No 60575 is available for lowering the 220 volt power source of many countries to 110 volts thus enabling the user to employ either a 50 or 60 Hz Fibrometer The frequency Hz rating of the Fibrometer must agree with the local line fre quency Up to ttvo Fibrometers and one Thermal Prep Block may be connected tb a single transformer e 2 RET Wn 2 This machine is intended for use in countries with 50 cycle Heftz alternating current 15 UM kS Ne re 207 9 Laboratory Requirements The Fibrometer operates on a standard 117 volt A C power source which must meet the National Electrical Manufacturers Assoc
35. accuracy s 57 SECTION EIGHT TROUBLE SHOOTING AND REPAIR TROUBLE SHOOTING AND REPAIR i This Section includes the following 1 2 3 4 5 Flowcharts for trouble shooting the Fibrometer 9 Additional problems with the Fibrometer i Trouble shooting charts for the Thermal Prep Block Trouble shooting charts for the Automatic Pipette Repair procedures for the FibroSystem In the past we have found the following types of iAdividuals doing trouble shooting and repair 1 e I The technician without any experience beyond operating laboratory equipment f you fall into this category DO NOT attempt internal repairs When working with the Flowcharts STOP at the large black dot and seek experienced help from the e repair technician where you work or through the BBL Hot Line The technician with training to make minor repairs f you fall into this category you must be the judge of the level of training you have acquired Obviously complex cir cuit work thermostat electrical evaluation etc are more work of the specialist Whenever you are not sure consult with the repair technician where you work or call the BBL Hot Line The trained repair technician Most repairs on FibroSystem equipment are within the range of activities performed by trained repair personnel Should there be any questions call the BBL Hot Line Our technicians and engineers will be
36. c instances as described in the section titled Trouble Shooting All internal arms levers and movable parts are designed to operate dry The motor is permanently sealed and lubricated To open this unit may invali date your warranty 22 SECTION THREE THE AUTOMATIC PIPETTE ver 23 THE AUTOMATIC PIPETTE 1 i USE The Automatic Pipette BBL Catalog No 60420 is a hand held pipetting device manufac tured specifically to function with the Fibrometer This device mechanically dispenses aliquots of liquid A quick and simple adjustment will allow the Automatic Pipette to take up and deliver either 0 1 or 0 2 ml aliquots Careful design and rigid testing assure the user of accuracy well within the 5 volumetric accuracy required for coagulation studies PRINCIPLES OF OPERATION The Automatic Pipette is a modular FibroSystem unit When connected to a Fibrotheter the pipette will electrically activate the Fibrometer at the same moment in each test When the final bit of activating reagent is dispensed into the FibroTube Cup there is a built in to 1 8 second delay which is followed by the automatic dropping of the Fibrometer probe arm Sweeping and timing activities wil begin automatically i The Automatic Pipette has a dual Purpose 1 Switch OFF itis a conventional pipetting device 2 Switch ON it dispenses liquid and activiates the Fibrometer The Automatic
37. ck has reached operating temperature You will need 0 2 ml of FibroPlastin9 per test Assume you are running TWO tests Using the Automatic Pipette take up and dispense 0 2 ml of FibroPlastin Into one cup labled Reagent Repeat this procedure a second time for the second Reagent cup NOTE A new FibroTip should be used for the FibroPlastin DO NOT use the same e for 10 11 14 15 FibroTrol and FibroPlastin Place both Reagent cups into the rack over a shallow well in the Prep Block It will take three minutes for room temperature liquids to reach the test t mperature 37 2 0 5 C Allow refrigerated liquids five minutes Once the liquids are at operating temperature place one Reagent cup irito the center reaction well of the Fibrometer Be certain to replace the FibroTip the Automatic Pipette Switch the pipette into the 0 1 ml delivery mode Do this by turning the plunger until the single notch aligns with the indicator mark Take up 0 1 ml of prewarmed FibroTrol with the Automatic Pipette i Switch the Automatic Pipette to the ON position f T is suggested that you add an additional 0 1 ml of FibroTrol to the control cup to give 8 total volume of 0 3 ml This will avoid pipetting the dry on the second run A i 1 Li 45 M ment e 16 Dispense 0 1 ml of FibroTrol into the Reagent cup now sitting in the
38. eter is properly detecting and timing fibrin strand formation If a problem is noted in these test runs please call BBL Technical Services toll free 800 638 8663 in Maryland call 3 1 666 0100 collect 1 Put the Fibrometer power switch in the ON position switch up 2 Wait approximately ten minutes for the machine to reach proper cator light will come on and re gmain on as long as the machine is at the correct operating temperar ture 17 PNA Nay ia Pi eon vk vramit i 3 Raise the probe arm to the UP rest position When the machine is not in use the prqbe should be left in the DOWN position to pro tect the electrodes from accidental damage Please note that the probe cannot be forced down into the probe sleeve One must switch on the machine and depress the Timer Bar to drop the probe into the DOWN position 4 The Fibrometer is an independent unit It is capable of prewarming reagents and samples Place six FibroTube cups into the warming wells Using a felt tip marker label one cup Control Plasma one cup Patient Plasma and the re maining four cups Reagent 5 Pipette approximately 0 3 ml of FibroTrol Control Plasma BBL Catalog No 40777 or BBL Catalog No 40778 into the control cup and if desired approximately 0 3 ml of patient plasma into the patient tube 6 Pipette 0 2 ml FibroPlastin Re agent BBL Catalog No 40775 or BBL Catalog No
39. f the Fibrometer was also not receiving power initially then the female receptacle must also be checked 2 If the Prep Block still does not function check the female receptacle If defective have BBL repair or order BBL Catalog Number 76014 Female Plug assembly Automatic Pipettes Repair is not done by BBL Defective pipettes are replaced See the repair policy The user will find that most problems with the Automatic Pipette can be solved by following the directions given in Section Seven of this manual 76 J REPLACEMENT PARTS LIST Replacement Parts List THERMAL PREP BLOCK 60419 CATALOG NUMBER 76013 76014 76015 76016 76017 76018 DESCRIPTION Bottom Cover Assembly Female Plug Assembly Heat Pad and Lug Assembly Male Plug Assembly Printed Circuit Board Assembly Thermostat and Lug Assembly 4 77 i i 1 t 1 Loos 1 1 REPLACEMENT PARTS LIST See TE TAR 70605 aues er 70630 B e Replacement Parts List Fibrometer9 60415 E CATALOG NUMBER DESCRIPTION E 60588 Fibrometer 9 Line Cord E 70585 Thermostat T ALSO ORDER 70609 Thermostat Phenolic Sleeve 70605 Light Indicator 70615 Capacitor Time Delay 30 mfd 70624 Counter Veeder Root ALSO ORDER 70669 Counter Trim Plate 70691 Counter Shim 70694 Counter Spacer 70910 Screw 4 40 x 2 70919 Washer 4 70639 Fuse 1 ampere 70928 Button Reset 78 Ares n net za tm nme
40. factory in its operations call the BBL Hot Line 800 638 8663 in Maryland call 301 666 0100 collect 7 Electrode Length Adjustments A Adjustments for short times Fibrometer stops 0 5 to 3 0 seconds after the probe drops NOTE Before making any adjustments test with an empty FibroTube Cup If the machine runs put 0 2 ml of reagent in the Cup and test use only the 0 2 ml of reagent If the Fibrometer runs proceed with adjustments If it does not run on either of the above tests call BBL 1 Remove the Probe assembly and hold it in your left hand so that the black cap is pointing upwards See Figure 8 1 2 With your right hand grab the probe foot the heavy wire ex tending down from the base of the probe and pull down with 0 9 to 1 4 Kg of pull 3 Watch the hook end of the moving electrode as you pull down on the probe foot The moving electrode will now be shortened by your pulling action This should solve most short timing problems 4 Check electrode lengths by TWO H methods a Replace the probe Dispense 0 3 m of reagent only into FibroTube Cup Place a cup into the reaction well With the Fibrometer switch in the ON position depress the Tim er bar to activate the machine If the probe does not drop and the detection and timing mechanisms do not begin to a function repeat steps 1 and 2 If this does not correct the problem call BBL Hot Line 800 638 8663 b Perform a
41. free labora tory grade absorbent tissue or bibulous paper NOTE This tissue or paper MUST be lint free The electrode must be free of jint and all other forms of contamination for proper fibrin strand detection A distilled water wash di rected from a wash bottle may be used to clean the electrodes If an acid wash is desired use 1 0 phosphoric acid followed by a distilled water rinse The use of other acids may pit the electrode 13 Set the timer to zero by firmly depressing the red reset button for at least one second 44 Repeat the Prothrombin Time Test Steps 7 through 13 Pipetting Hints Proper and consistent pipetting is necessary for sustained accuracy Regardless of which sys tem you use the Automatic Pipette or standard pipetting adopt the following procedure A Rest the pipette on the lip of the cup that is closest to the center of the Fibrometer m DO NOT let the tip touch the opposite side wall or the reaction mixture When dispensing insert the pipette tip at least 1 4 of the way intoithe FibroTube APT B cup C Aim the tip at the back bottom of the FibroTube cup D Eject the material smoothly and without splashing j pA DO NOT allow the liquid being pipetted to run down the side wall This will cause an unequal liquid level and result in an erroneous short end point WA 1 ette or FibroTip can b used for aa MUST be used aA dn go AVOID PIPETTING CONTAMINATIO
42. happy to assist you Please note that a wiring diagram and mechanical diagram have been included in this manual A complete parts list has also been provided i NOTE BEFORE STARTING ANY REPAIRS CHECK YOUR WARRANTY i OBSERVE ELECTRICAL SAFETY UNPLUG THE POWER CORD BEFORE STARTING ANY REPAIRS i ALSO WIPE THE MACHINE TO REMOVE ANY PLASMA RESIDUE A HEPATITIS DANGER EXISTS e oA ST MN oo P4 jq cuam 61 FLOWCHART NUMB PROBLEM NO POWER Machine Fails to Warm and Motor Will Not Operate Fibrome Cord Plugged In Cord Not Plugged Plug in Cord to grounded 3 wire receptacle Check Power Switch Switch is OFF or Not Fully in ON position FIRMLY move the Power Switch to the ON position Check Fuse 70639 Fuse 15 Good the thin wire in the glass tube is not melted into two sections Fuse is Blown Can replace HOWEVER this an indication one or more Machine components have failed Call BBL Call BBL Hot Line 62 PROBLEM Check Pipette Cord Plug Plug is Secured Properly Activate with Timer Bar Machine Activates Pipette is faulty Call BBL for FREE REPLACEMENT 63 Machine is Not Activated by Automatic Pipette Both the Fibrometer and Automatic Pipette Switches MUST ON position FLOWCHART NUMBER 2
43. iation standard of 99 5 to 128 7 volts The outlet must be a standard laboratory three wire grounded receptacle Other power sources or outlets may damage the machine The working environment should be as clean and dust free as possible with an ambient temperature between 18 and 32 C 64 4 to 89 6 F Procedures The following procedures are designed to allow you to set up and test your Fibrometer step by step It is recommended that you test this unit independently of the Thermal Prep Block and the Automatic Pipette If you plan to connect two Fibrometer timers to a single power source each unit should be tested independently If any problems occur as you test your new Fibrometer please call the BBL Hot Line toll free 800 638 8663 in Maryland call 301 666 0100 collect 1 Begin by placing the Fibrometer box with the words OPEN THIS END in the UP position Take out the packing material and remove the machine Included in this one shipping carton are A One Fibrometer B One line cord C One 0 3 ml probe D 400 FibroTube cups Visually inspect all items for any indication of shipping damage WARNING The 0 3 ml probe is packed in the DOWN position i e in the probe sleeve DO NOT turn the Fibrometer over with the probe still in the machine There is no mechan ism to keep the probe from falling out of the well and away from the base 2 Place the machine on a level bench top that is free from vibration At this
44. ic End Points 2a Any inconsistency in test procedures or reagents will cause erratic timing Probable Cause This condition is See Section II rare and is usually a technique re 2b If the problem is low line voltage agent or line voltage problem check the Installation Require ments Section 11 In addition have an electrician periodically check line voltage 3 Sticky Timer or Digit Repetition 3 See Repair Procedures 10 and 11 Machine works on Prothrombin Times 4 See Flowchart 5 but does not pick up clot on PTT s TROUBLE SHOOTING MEME THERMAL PREP BLOCK PROBLEM SOLUTION o 1 Machine will not heat Probable Causes _ A Machine not switched ON 1a Firmly move the power switch ON 4 B Faulty power connection 1b This problem may occur when the 3 Prep Block is connected to the Fibre meter See Repair Procedure 12 9 2 Erroneous Temperature 2 The heating element and system of the Prep Block is almost identical to the Fibrometer See Flowcharts amp 4A to 4C Also see Repair Proced ure 3 70 vt TROUBLE SHOOTING AUTOMATIC PIPETTE Many of the problems with the Automatic Pipette can be solved by reading Section VI Helpful Hints PROBLEM 1 Fibrometer is not activated by the Automatic Pipette 2 Plunger binds or will not depress fully Probable Causes A Dirty cannula B Worn O Ring 3 Leakage of liquid from tip Probable Causes A Warn or defective
45. is placed in the UP position This allows for easy cleaning in sertion of FibroTube cups and pipetting KTEB For details concerning the probe drop and electrode movement see COMPONENT Figure 2 1 The Probe Unit PART PRINCIPLES OF OPERATION The Electrodes Two durable metal electrodes made from 304 stainless steel are the only parts of the Fibro meter that make contact with the reaction mixture There is a moving and a stationary elec trod The moving electrode cycles through the reaction mixture by sweeping in elliptical pattern A small hook on the tip of this electrode will pick up the initial fibrin thread mono mer The stationary electrode is responsible for creating an electric potential between the electrodes When the probe is in the DOWN or sensing position the stationary electrode remains in the reaction mixture The Probe Foot The heavy wire extending from the base of the probe is the probe foot The moving electrode is an extension of this probe foot A cam raises and lowers the probe foot thus moving the electrode The probe foot also is the contact point between the moving electrode and the de tection circuit of the Fibrometer M 11 i PROBE ARM MOVING ELECTRODE STATIONARY ELECTRODE REACTION WELL WARMING WELLS 6 INDICATOR LAMP READOUT RESET BUTTON DIGITAL READOUT INDEXING DOT POWER CONNECTION TIMER BAR ON OFF SWITCH PLUG IN JACK OUTLE
46. ld extend approximately 1 6 mm into the probe hole beyond the sleeve If it extends more than 1 6 mm 1 Use a 6 4 mm blade screwdriver to press the lever back into the cavity Press until a stop is felt 2 Carefully apply 2 3 to 4 5 Kg of pressure to force a slight bend in the lever The lever is malleable bronze and may easily be bent back if the desired point is overshot 3 Check the extension Repeat procedure until the desired 1 6 mm is achieved 4 If the lever extends less than 1 6 mm put the screwdriver behind the lever and carefully apply outward pressure until the desired extension is reached C The probe will not remain the UP position if the lever extends less than 1 6 mm be Li yond the sleeve See Step 4 above i 2 Measuring the Heat Block Temperature Fibrometer A Method I 1 Pipette 0 5 ml of water into FibroTube 9 Cup and place the FibroTube Cup into the reaction well of the Fibrometer 2 Allow at least ten minutes for temperature stabilization j 3 Use a 30 copper constant thermocouple and potentiometer type millivoltmeter preferably one calibrated in C and having a compensated refer nce junction 1 Wind a small coil 6 4 mm long of the thermocouple wire near the hot junction on 4 a 3 2 mm mandrel and insert into the FibroTube Cup 4 Allow five minutes for stabilization then read the temperature i B thod Il 1 Pipette 0 5 ml of water into
47. moving electrode You should see it move slightly forward 56 MOVING ELECTRODE 7 IN RAISED POSITION ES ya E E p A B C CORRECT BBL INSIDE DIAMETER INSIDE DIAMETER FIBROTUBE 9 TOO SMALL TOO LARGE CUP FLUID LEVEL Figure 7 3 and up This shortens the electrode by 1 30 or 1 93 mm Test the adjustment by placing 0 3 ml of reagent only in a FibroTube cup in the center well of the Fibrometer Place the probe in the Fibrometer and press the timer bar so that the probe will drop into the reagent It should continue to run In the event that the unit again stops prematurely the procedure can be repeated one more time If the problem cannot be resolved call the BBL Technical Services toll free Hot Line at 800 638 8663 in Maryland call 301 666 0100 collect A detect condition can be caused by the accidental bending of the moving electrode away from the stationary electrode This shortens the electrode causing the no detect con dition If the thin wire section is bent it may be straightened with a pair of forceps If the thin wire section is straight but the tubing is bent it can usually be corrected by placing your thumbs against the tubing at the black cap and pressing the tubing against the cap Another factor that can cause no detect condition is that the automatic pipette is dirty and not delivering full volume Cleaning the pipette as described in Section Six will restore the volumetric
48. o minimize maintenance problems the plunger is molded from a chemical resistant plastic and has a neoprene rubber O ring and a stainless steel barrel coupling 3 Once the Fibrometer action has been initiated the Pipette switch can be moved to OFF without affecting the Fibrometer This feature permits additional dispensing of reagents while the Fibrometer is in Operation 31 SECTION FOUR THE THERMAL PREP BLOCK 33 THE THERMAL PREP BLOCK USE An integral component of the FibroSystem is the Thermal Prep Block This precision instrument has been designed for prewarming large numbers of cups and tubes containing reagents and patient specimens for Fibrometer testing PRINCIPLES OF OPERATION The Prep Block provides thirty wells for prewarming Twenty shallow wells are constructed to hold FibroTube cups and ten deep wells exist for 12 x 75 mm test tubes The Prep Block ensures that liquids are prewarmed to the required 37 2 0 5 C before testing When used as directed this instrument is an efficient and convenient device for warming large quanti ties of all liquids to be used in the tests run with the Fibrometer The thermal control system of the Prep Block has a very narrow differential It shouid never require adjustment Solid state circuitry maintains the temperature specified in liquids i in both the shallow and deep wells as long as the ambient temperature is between 18 32 e By
49. olled dimensions to ensure that the proper liquid level is maintained in the Fibrometer reaction well Second they are made of special materials that allow the proper heat transfer from heating block to liquid Finally these cups are one time disposables which eliminate errors due to contamination The recommended pipette tip for use with the Automatic Pipette is the FibroTio TM Dispos able Pipette Tip FibroTip Disposable Pipette Tips should always be used when pipetting with the Automatic Pipette These chemically clean disposable tips are easy to use and eliminate the hazards associated with mouth pipetting 1 f BBL COAGULATION REAGENTS BBL manufactures a broad line of reagents as part of the FibroSystem When these reagents are used as directed any laboratory can conveniently perform the most modern coagulation tests Complete and detailed procedures for these tests are provided in the package insert which accompani s each product All personnel should read the insert before using the product A complete list of BBL coagulation reagents is shown in the chart on the next page ES i tte TD BBL COAGULATION REAGENTS 40775 76 t FibroPlastin Rabbit Brain Thromboplastin with Calcium 40821 22 t FibroPlastin 11 Liquid Rabbit Brain Thromboplastin with Calcium 40777 40778 t FibroTrol Citrate Normal Coagulation Control Plasma t FibroTrol Oxalate Normal Coagulation Control Plasma 40779 80 t FibroLe
50. orrected at the factory It should be noted that the length of time a corrected counter will remain operative is unpre dictable For more detail call Technical Services at BBL i Sticky Timer If your Fibrometer has a sticky timer call BBL for a loaner Hold this loaper for several days until you are sure the procedure outlined below has corrected the problem The prob 1 i 75 i t lem is caused most often by a piece of plastic flash from one gear breaking off and wedging into another gear A Remove the prbbe and activate the machine B Allow the machine to run for two hours Punch the reset button every fifteen to twenty minutes during this running time This may cause the bit of plastic that is causing the jamming to drop out C If this fails or is only temporary call the BBL Hot Line D If your own inspections and tests show that a new counter is required order BBL Catal Number 70624 and the required hardware noted in the parts list 12 Faulty power connection Fibrometer and Prep Block Modular If the Thermal Prep Block will not heat and the switch is in the ON position A Remove the Fibrometer line cord from the wall receptacle B Separate the Prep Block from the Fibrometer C Plug the female line cord plug directly into the Prep Block Plug the line cord into th wall receptacle 1 If the Prep Block works check the male plug on the Fibrometer If defective h ve BBL repair it I
51. ot b din or will be below 37C There is little chance of a Prep Block being only one or two legrees off the desired range b i 65 n PROBLEM Machine Receiving Power Check Thermostat 70585 Thermostat Is Not Faulty Call BBL Hot Line Fibrometer Not Heating At All Is Machine Receiving Power SEE REPAIR PROCEDURE 3 66 FLOWCHART NUMB Fibrom Machine Is Not Receiving Power SEE FLOWCHART NUMBER 1 Thermostat Is Faulty Replace Thermostat 70585 and 70609 or return to BBL for repair FLOWCHART NUMBER 4C Fibrometer Heating to Less Than 36 8 Fibrometer PROBLEM Check Ambient Temperature Ambient Temperature Too Low Ambient Temperature Between 18 C and 32 C j Regulate Ambient Temperature Between amp 18 C and 32 q Check Thermostat 70585 SEE REPAIR Y PROCEDURE 3 i Thermostat Thermostat Is Is Not Defective Defective Replace entire Thermostat Call BBL Hot Line 70585 and 70609 or send _ to BBL for repair pe 1 pe oe 0 Ape n 67 FLOWCHART NUME PROBLEM Fibrometer Does Not Stop with Fibrin Formation Does Not Detect End Point Fibra wi Check Electrode Alignment Electrodes Misaligned Electrodes Aligned
52. ow you to test your new Thermal Prep Block It is assumed that the Fibrometer and Automatic Pipette have been tested independently If any problems occur as you test your new Thermal Prep Block please call the BBL Hot Line toll free 800 638 8663 in Maryland call 301 666 0100 collect er 1 Begin by placing the Thermal Prep Block box with the words OPEN THIS END in the UP position Lift out the machine and visually inspect all items for shipping dam 2 Pi amp ce the machine on a level bench top which is free from vibrations If the Prep Block is to be used as a part of a modular system be sure to leave room for additional pieces of equipment 38 ku all nee sa a nr nn Ran al A a kad n oe 3 To utilize the modular plug in design set the Prep Block to the right of the Fibrometer Carefully cennect the three pronged outlet on the right side of the Fibrometer to the receptacle on the left side of the Prep Block WARNING DO NOT twist these outlets Maris 4 Plug in the line cord from the Fibro meter and put the power switch of the Prep Block into the ON position en Ve FD we wy a ee we ee ee Sel ka ONE 5 Wait approximately fifteen minutes for the machine to reach operating temperature The indicator light will i be ON while the machine is warming At operating temperature the light will be OFF 6 The Prothrombin Time Test can now be r
53. p fall out and leakage when using the BBL Catalog No 60420 Automatic Electric Pipette or BBL Catalog No 60422 Nonelectric Pipette The twisting motion of inserting the disposable plastic pipette tip into the barrel of the pi pette may leave bits of plastic adhering to the side walls of the hole in which the tip js in serted This prevents the tip from seating properly and may result in tip fallout OE Cleaning the ring and cannula is required to prevent deformation of the soft as it rides over dirt particles Deformed worn or damaged O rings may permit air to enter and result in leakage of material from the tip BBL Catalog No 70312 FibroTube Disposable Plastic Coagulation Cups are specifically recommended for use with the Fibrometer 9 and Automatic Pipettes because they are designed to provide precisely the liquid level height required by the Fibrometer for accurate Operation The BBL Catalog No 70314 FibroTip is precisely engineered and rigidly quality controlled for a leakproof fit in the 60420 and the 60422 pipettes i TH IS eT un 49 MAINTENANCE 1 Hold the pipette as one would when dispensing With the other hand grasp the metal barrel and pull it straight off the pipette 2 Take the metal barrel to another bench away from the plastic parts Place a small quantity of acetone on a swab and vigor ously scrape around in the hole where the tip is placed This will clean ou
54. r more than ten years of successful use and millions of field determinations the Fibrometer is a time tested device The Fibrometer is a rugged unit which ensures the quality performance demanded by mod ern laboratory standards When used for tests ending with fibrin formation this machine i is capable of sustained accuracy consistency and reproducibility The performance characteristics and specifications are detailed on the following page A CA THE FIBROMETER PRECISION COAGULATION TIMER Performance Characteristics and Specifications Heating Temperature Detector Cycle Time Machine Resolution Timing Range Standard Deviation 9 Dilution Curves Test Volumes Patient Sample Size Liquid Heating Times Dimensions and Weights Machine Warm Up Time Electrical Specifications 37 2 0 5 0 5 ste 0 5 sec 0 0 to 999 9 sec 0 3 sec for times under 25 0 sec 0 6 sec for times over 25 0 sec Curves established using the Fibrometer have the same characteristics as those obtained by visual methods 0 3 m and 0 4 ml 0 1 ml or 0 2 ml of patient s plasma whole blood or occasionally serum Refrigerated fluids 5 minutes Room temperature liquids 3 minutes Height probe up 178 mm 7 Width 102 mm 4 Depth 184 mm 7 25 Net Weight 3 Kg 7 Ibs Shipping Weight 4 8 Kg 10 5 Ibs Approximately 10 minutes 117 volts 60 Hz 0 6 amps Catalog No 60415 117 volts 50 Hz 0 6 amps
55. rk should be done bya qualified electrician DO NOT under any circumstances cut or remove the grounding prong from the plug UNPLUG the power cord before cleaning or performing any of the procedures described in the section entitled Trouble Shooting DO NOT perform any servicing except for those items specifically stated and described in this manual Advice on specific technical problems can be obtained by contacting the Tech nical Services Department at BBL Post Office Box 243 Cockeysville Maryland 21030 or telephone toll free 800 638 8663 in Maryland call 301 666 0100 collect DO NOT open any instrument cover under any circumstances To do so may invalidate your warranty DO NOT remove labels or warning tags from this equipment Use FibroSystem Reagents only as directed in the package insert accompanying each product no Set Mina Cnm la VE Parra a MAT a tla SIN Ct T e ee a me e aaa ms 3 SECTION ONE FIBROSYSTEM THE TOTAL SYSTEM FOR COAGULATION TESTING FibroSystem the registered trademark of Becton Dickinson and Company is a total system containing all the equipment reagents and disposable supplies needed for accurate coagula tion testing The FibroSystem concept provides a reliable method for performing any labor atory test which utilizes a fibrin clot as the end point Its range of operation includes diag nostic evaluation monitoring anticoagulation therapy and measuring
56. s of the Prep Block In this position the cups rest on the spherical bottoms of the wells just as they would without the trays The trays do not affect the temperature of the cup and its contents 5 2 Placing cold liquids in wells adjacent to wells with liquids at 37 2 C does not disturb the thermal equilibrium of the heated liquids 3 Reagents for the next day can be prepared and stored overnight A box of ten trays BBL Catalog Ko 70313 may be purchased The required number of cups can be placed in the trays 0 2 ml of reagent added to each cup and the readied trays stored overnight at 4 C The trays are made to be stacked to use less storage space 40 i i i i E MAINTENANCE PRECAUTIONS For Laboratory Use The Thermal Prep Block is easy to keep clean Wipe the metal surfaces and wells with a damp cloth Be sure to take all necessary precautions to avoid hepatitis dangers when working with human serum plasma or whole blood The wells should be wiped periodically There are no temperature controls or adjustments of any kind in or on the Prep Block The heating circuit is extremely stable If there is a component in this circuit the block will become too hot to hold or will be well below 37 C 41 SECTION FIVE STANDARD OPERATING PROCEDURE PROTHROMBIN TIME TEST 43 PROTHROMBIN TIME TEST i i i This section contains a step by step procedure to test the
57. t Activated Platelet Factor Reagent 40802 40805 40813 40823 40631 40636 40637 40662 40638 40639 40640 t FibroTrol 10 20 Citrated Abnormal Range Coagulation Control Plasma Kit t FibroTrol 10 20 Oxalated Abnormal Range Coagulation Control Plasma Kit t FibroFac Correction Reagent Kit BART TM Reagent t Platelet Factor Reagent t Prothrombin Free Plasma Bovine Soarce Sodium Oxalate 0 1 Molar Calcium Chloride 0 0032 Molar Calcium Chloride 0 02 Molar Calcium Chloride 0 025 molar t Thromboplastin Generation Kit for performing the Thromboplastin Generation Time Test 40641 42 t ThromboTest Owren Prothrombin Time Reagent Trademark of Nyegaard amp Co A S Oslo Norway t Refrigerate Store at 2 to 8C Single Pair of Fibrometer and Pair of Fibrometer Pair of Fibrometer Units with Fibrometer Fibrometer Thermal Prep Units with Thermal Pair of Thermal Prep Block Units Units Block Unit Prep Block Unit Figure 1 1 The Modular Concept THE MODULAR CONCEPT Due to the small amount of electricity used by the FibroSystem components and their special design features modular combinations are possible Built into opposite sides of both the Fibrometer and the Thermal Prep Block are special polarized three pronged plugs and receptacles This means that any of these machines can be plugged into any other Fibro meter or Thermal Prep Block The first machine will obtain power from one three wire line cord plugged into a
58. t the plastic bits which have accumulated from normal usage 3 Use a fresh swab moistened with alcohol and swab out the center hole in the metal barrel Use a small swab and keep cleaning until the swab comes away clean Rinse the barrel and cannula with distilled water to wash out any particles that remain Dry thoroughly 50 4 Turn the plunger of the pipette to the 0 2 ml setting and depress the plunger fully This will expose the O ring With a lint less tissue moistened with alcohol clean the area around the O ring and the O ring itself When clean visually examine the ring for slits flat spots and other signs of wear If there is damage replace the ring or request a replacement from BBL 5 Place a small amount of silicone grease or petroleum jelly around the O ring With the pipette plunger stil depressed slide the piston and O ring into the tube of the metal barrel Align the barrel so that the notch on the perimeter matches the projection on the pipette Snap the barrel in place 6 Work the plunger up and down to lu bricate the tube Take a tip insert it in the pipette and pick up 0 2 m of water Hold the pipette steady for one minute without touching the plunger watching the end of the tip to see that no drop forms Repeat po on the 0 1 mi setting rv SECTION SEVEN USE OF FIBROTUBE DISPOSABLE PLASTIC COAGULATION CUPS WITH THE FIBROMETER
59. the Fibrometer 1 The Pipette is designed to be cradled in the hand with the forefinger on the top rest This allows for maximum flexibility and control when dispensing Many users prefer to hold the Pipette in handgun fashion Either approach will produce the same re sults A few simple tests will determine which is most comfortable for the operator Figure 3 2 2 Insert the plug on the end of the pipette cord into the jack located at the lower left front of the Fibrometer 3 Seat a FibroTip onto the forward end of the pipette Use a firm turn ing motion as shown here Figure 3 3 ALIGNMENT INDICATOR 4 To adjust for the volume of pick up and delivery turn the plunger in either direction to align the calibration notches with th alignment indicator One notch equals 0 1 ml two notches equals 0 2 ml Figure 3 4 5 With the Fibrometer switch the ON position and the Pipette switch in the OFF position fully depress the plunger Insert the tip into the liquid reservoir NOTE Be sure to fully depress the plunger before the tip is placed into the liquid This will prevent FULLY aeration of the liquid and the subsequent for RETRACTED mation of error producing air bubbles NC DEPRESSED REM LE j 0 2 FULLY DEPRESSED i i 9i Figure 35 6 lAllow the plunger to retract fully and pick up the aliquot of liquid into the FibroTip
60. the effect of heparin and protamine sulfate on whole blood during open heart surgery renal dialysis etc EQUIPMENT 1 Fibrometer Precision Coagulation Timer The major component of the FibroSystem is the Fibrometer Precision Coagulation Timer In this manual this component will be called the Fibrometer The instrument is designed to automatically detect fibrin clot formation 2 Thermal Prep Block The Thermal Prep Block complements the Fibrometer This unit is a precise device for pre warming reagents and plasma to testing temperature Temperature equilibrium is maintained at 37 2 0 5 C in 20 shallow wells and 10 deep wells The shallow wells will accommodate Fibro Tube Coagulation Cups The deep wells have been designed to hold standard 12 X 75 mm test tubes 3 Automatic Pipette The third component in the FibroSystem is the Automatic Pipette which is capable of dispensing the 0 1 ml and 0 2 ml volumes normally used in coagulation testing Once plugged into the Fibrometer the Automatic Pipette will serve a double function This hand held pipetting gun will accurately pipette liquids and it will automatically initiate the meter clot detecting and timing mechanisms DISPOSABLE SUPPLIES The recommended reaction cup for use with the Fibrometer and the Thermal Prep Block i is the FibroTube Disposable Coagulation Cup Three factors make these cups an important FibroSystem component First they are made to carefully contr
61. ts 22 The probe arm of the Fibrometer will automatically drop Sweeping action and timing will now begin automatically The time for the first fibrin strand detection in the FibroTrol plus FibroPlastin reaction mixture should be between ten and thirteen seconds for 100 plasma 23 Repeat the above test to verify the results Jhen test the patient s plasma 30 Heca co tag iyan ee n TPT noc PRECAUTIONS For Laboratory Use TIP CONTAMINATION disposable tips when going from one reagent or Concentration to another In the Prothrombin Time Test the recommended tip replacement schedule is a One tip for the FibroTrol b A new tip if patient s plasma is to be tested c A new tip for the FibroPlastin Reagent d A new tip to dispense the FibroTrol aliquot 0 1 ml into the FibroPlastin CAUTION Any tip coming into contact with patient serum plasma or whole blood is a potential HEPATITIS DANGE R Avoid personal and environmental contamina tion Discard all FibroTip Tips with the Same care as used for any contaminated Pipette ADDITIONAL DESIGN CHARACTERISTICS 1 After depressing the plunger to dispense some liquid will remain in the FibroTip This condition was compensated for during the calibration of the pipette This liquid DOES NOT affect the accuracy of the Automatic Pipette 2 The material being pipetted never contacts with the plunger or barrel assembly No daily disassembly or cleaning is required T
62. u insure it Without the insurance slip there is no chance of tracing the shipment if it is lost Automatic Pipettes og BBL does not repair defective Automatic Pipettes it exchanges them Refer to the discussion under Warranty An exchange unit will be airshipped to you The defective unit must be returned so that it can be repaired and placed in the ex change pool 90 REFERENCES 1 Fewell R G A Cundy and G C Jenkins 1972 The One Stage Prothrombin Test A Comparative Study of Mechanized Prothrombinmeter and Manual Testing Med Lab Tech 29 147 2 Miale J B 1965 The Fibrometer System of Routine Coagulation Tests Amer J Clin Pathol 43 475 3 Sterling R E A A Wilcox A G Ware and M K Umehara 1965 Improved Instru mentation for the Determination of Prothrombin Activity Clin Chem 77 409 4 Barnett R N and C L Pinto 1966 Reproducibility of Prothrombin Time Determin ations Between Technologists Am J Clin Pathol 46 148 5 Viral Hepatitis 1973 World Health Organization WHO Tech Rep Serv 512 Page 820 91
63. un to test the performance of the Thermal Prep Block E m ES 1 Check that both instruments are at operating temperature B Place four FibroTube cups into ut one of the removable plastic trays B Label cup FibroTrol and the remaining three cups Reagent Place the tray into the well of the is Block 39 pa ma C Utilizing standard operating pro cedures with the Automatic Pipette fill one cup with 0 3 ml FibroTrol and the remaining cups with 0 2 ml FibroPlastin Reagent D Wait three minutes for room temperature liquids to arrive at the testing temperature Allow refrigerated liquids five minutes E Place one cup with 0 2 mi of FibroPlastin into the center reaction well of the Fibrometer With the Pipette switch ON de liver 0 1 ml of FibroTrol into the cup F After the built in delay the probe arm of the Fibrometer wil fall and the sweeping and timing actions will begin The time for the first fibrin strand detection should be between eleven and thirteen seconds G Repeat the above procedures to verify the results You are ready to begin routine use of the Thermal Prep Block There are NO temperature 8t tings or day to day calibrations necessary with this or any other FibroSystem instrument OPERATING HINTS 1 Ten FibroTube cups may be placed in one removable plastic tray Two trays are designed to fit snugly across the shallow well
64. y active CONTACT gt i The probe or electrode foot FINGER CAM CONTACT PORTION is contacting the cam carry DETECTION h ing the signal that a clot is CIRCUIT present to the cam cam finger and detection circuit iy Figure 7 1 55 In Figure 7 2 the moving electrode is mechan ically searching for a fibrin strand The elliptical path taken by the movable electrode is directed and controlled by the groove in the black cap where the upper portion of the electrode is captivated The probe foot is resting on the insulated por tion of the cam The moving electrode is now electrically inactive and cannot conduct As the cam continues to rotate the moving elec trode is lifted by the contact portion of the cam and the configuration returns to that of Figure 7 1 NOTE The moving electrode is alternately in LIQUID LEVEL the up or down position every half second stopping Fibrometer only where there is a clot to complete the circuit M PROBE FOOT i 1 4 CAM INSULATING PORTION Figure 7 2 1 Important If 0 3 ml total volume is placed within a competitive cup whose internal diameter is slightly smaller than the internal diameter of the BBL 9 FibroTube cup the liquid may rise to a higher level and bridge the 1 27 mm gap thereby closing the circuit causing the Fibro meter to stop almost as soon as the probe electrodes drop into the cup Figure 7 3 Converse

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