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Agilent Instant Pilot G4208A

1. OFF 0 000 ml min E OFF OFF OFF Toggle OFF 400 bar Minimum Stroke AUTO Compressibility 100 10E 6 bar Max Flow Gradient 100 0 ml min G Primary Channel Auto x Exit Specifies a time limit for your analysis Ly 13 45 Figure 43 Method Screen Agilent Instant Pilot Users s Guide Working with Methods 2 71 2 72 Working with the Instant Pilot Loading a Method A method can be loaded pressing File in the Method screen 1 Enter the Method screen Press File ao o A W N Press OK or Load Select option 1 Load Select a method from the list The current parameters are displayed The Method Module screen lists all methods that are stored in the modules For each method there is a date when the method was last changed When a method is loaded it becomes the current method w Method File Load Location INTERNAL J Name LAB DEF INTERNAL WOLF 21 09 05 11 25 12 USB Load JR 15 04 Deletes the selected method Duplicates the selected method Protect unprotect a method and adds a lock icon Leaves this screen Loads a selected method Properties name comment ee Gee es BE change history Figure 44 Method File Load Screen Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Modifying a Method A method can be modified by changing the settings in the Method screen 1 Scroll to the line you want to change 2 Press E
2. apo B 2000 2000 0000 oft Agilent Technologies Notices Agilent Technologies Inc 2006 2015 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 64208 90008 Edition 04 2015 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Germany Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licen
3. 04 13 07 12 51 18 FLD Cell type 1 detected 8 04 13 07 12 51 04 Autosampler Thermostat disconnected 04 13 07 12 51 10 System G1315C PP00000024 detected 04 13 07 12 51 11 System G1311A4 DE23923124 detected 04 13 07 12 51 18 System G1316A4 DE14923865 detected 04 13 07 12 51 19 System G13214 DE92001563 detected 04 13 07 12 51 21 System G13294 DE9 1603245 detected End of Logbook Figure 27 Logbook Screen saved to USB Flash Drive Agilent Instant Pilot Users s Guide Start up Information 1 Configuration To view change the configuration press More from the Welcome screen and select Configuration from the menu g Configure System E to change the settings Edit i N opens the Setup Wizard AGP Remote No External Synchronisation Setup Time 09 54 Date SEP 13 2006 Auto turn on OFF Turn off on error No G leaves this screen Y Exit A 09 54 System I Controller if Quat Pump Attosampier system or module specific information Figure 28 Configuration of System To change the system configuration move to the line you want to change and press Edit After doing the changing press OK or Done The Instrument Name will appear as identifier on the screens e g Welcome or printouts reports To start the Setup Wizard see also Getting Started on page 31 press Setup in system To change a module specific setting select the appropriate module view Agi
4. Filter Quat Pump No analysis 13 28 49 x G Autosampler Thermostat disconnected 13 28 45 opens a menu to control certain itTc c Column ID left data valid 13 28 52 siti giTce Column ID right not present 13 28 52 Control System activities B Quat Pump Pump off 13 28 49 s aee Calibration done 13 28 52 gt prints the logbook to a file on Tcc Valve switched to column 1 13 28 52 print the USB Flash Drive B Quat Pump Composition ramp off 13 28 49 amp Quat Pump Flow ramp off gt 7 13 28 49 System G1315C PP00000024 detected 13 28 50 System G1316A DE14923865 detected 13 28 52 System G1311A DE23923124 detected 13 28 53 System G1321A DE92001563 detected 13 28 52 G leaves this screen G1314C JP92110261 detected 13 28 55 G1329A DE91603245 detected 13 28 56 Exit DEE System I Controller I Quat Pump TAutosampler bo system or module specific information Figure 26 Logbook Screen The Logbook screen is a configurable overview of the information internal sequences error maintenance system and Early Maintenance Feedback EMF messages To configure the view press Filter Control opens a menu to control certain system setting activities To leave the screen press Exit or Esc Press Print The logbook is saved to a connected USB Flash Drive The text is written and saved into folder PRINTOUT as LOGBOOK MHT or LOGBOOK HTM see Figure 27 on pa
5. Move to a line of the Injector Program and press Edit button to view the current settings or start a new line w Method Injector Program L E edits the selected line Line Function End of User Defined Injector Program Edit 1Draw gt inserts a program line 2Eject insert 3 Mix gt 4 Inject deletes a program line 5 Valve Delete 6 Needle gt 1 Move Vial 7 Wash exits the injector program 2 Remote gt without any changes 3 Contact gt a k 3 Wait ends the injector program 5 Repeat gt 7 6 Increment Pos 7 Reset Pos fey 10 Defaut J Syringe Home a Figure 60 Injector Program Setup Screen Agilent Instant Pilot Users s Guide 87 2 Working with the Instant Pilot Work dle Uo g with M Press the Insert button and select an action item w Method Injector Program Line Function End of User Defined Injector Program 1 Draw Def from Sample 2 Draw Def from Sample 2Eject gt 3 Draw from Vial 3 Mix 4 Draw from Seat 4 Inject 5 Draw from Air 5Valve gt Delete 6 Needle gt 7Wash gt 8More gt Figure 61 Injector Program Setup Screen Move to line End of User Defined Injector Program press the Insert button and select additional action items as required Via the Edit button you can change the parameters i Method Injector Program f LJ Line Function 1 Draw def
6. calibration 129 recalibration 133 multiple level calibration 129 multiple vial analysis 123 navigation keys 26 numeric keys 26 0 observing the chromatogram 120 Index offline work on methods 84 OK key 26 ON OFF of modules 56 online help 27 information system 27 on line information 26 on time 37 operating altitude 17 operation concept 30 operation temperature 17 overview keys and display 25 P parts adapter plate 163 CAN cable 163 identification 163 Instant Pilot 163 physical specifications humidity 17 line voltage and frequency 17 operating altitude 17 operation temperature 17 power consumption 17 safety standards 17 weight and dimensions 17 plot screen 101 rescale 103 signal unit axis 103 time axis 103 x axis 103 y axis 103 power consumption 17 power on signal 107 prepare signal 108 175 pressure format 33 preview sequence 94 printing 65 to USB Flash Drive 13 65 problems contact Agilent 162 product number change 54 properties method 39 78 sequence 38 40 protect methods 78 sequence 96 protect your method 13 protection sequence 96 PSI 33 ready signal 108 recalibration 129 alter multi 92 with same group of standards 129 recycling of mercury fluorescent lamp 17 removing Instant Pilot 24 repairing the Instant Pilot 163 rescale plot screen 103 resident system 146 restrictions 111 RS232 interface 107 running an isocratic analysis 115 multiple vial analysis 123 running a
7. LAN Subnet Mask 255 255 248 0 LAN Def Gateway 134 40 24 1 The address of the device on the network Jal 16 18 Figure 15 Getting Started Setup Wizard LAN setup Agilent Instant Pilot Users s Guide Start up Information to change the settings move to the line and press OK to edit the settings Then press Done to write down the new values closes the setup 1 35 1 36 Start up Information Finally the Welcome or the Configuration screen is displayed G1311A DE23923124 g Details G1329A DE91603245 Welcome SYSTEM1 Quat Pump Autosampler TGG G1316A DE14923865 DAD SL G1315C DE60755000 _ FLD G1321A DE92001563 Select a button to continue 10 40 Method Logbook Figure 16 Getting Started Welcome Screen The next time the Instant Pilot is started it will start with the Welcome screen To activate the setup wizard again select More 1 Configure System Setup to open the Setup Wizard or use System or Controller or a module to change the parameters Agilent Instant Pilot Users s Guide Start up Information 1 System Information To gather information about the Instant Pilot and the Agilent modules press the Details button from the Welcome screen g System Info amp updates the displayed Controller DE61800100 a i i Reload information Quaternary Pump DE23923124 Main Revision A 06 04 001 gt prints
8. Pit Setup f Selecta f Control Ext Figure 20 Status Screen Default Defined The Status screen is a configurable overview of the instrument status You can view actual values states and edit parameters The screen is divided into four tiles Each tile itself can also hold up to four smaller tiles The Instant Pilot automatically chooses the size of the tiles based on the selection The display shows the actual connected and powered up Agilent modules The title color gives the current STATUS yellow not ready gray ready green run red error The dialog title shows the instrument status in color and with text When this screen has not been setup before it will show from each module in the system one or more signals parameters For optimization of the view use Setup of a Status Information Screen on page 44 Agilent Instant Pilot Users s Guide Start up Information 1 Table8 Status Functions of Keys Button Description Plot shows different signals of the connected modules over time The signals are user selectable can automatically be rescaled for best on screen fitting Setup lets you set up the views Select one of the last 4 setups can be loaded Control opens a menu to control certain system activities for details refer to Instant Pilot s Info System 2 eae ES 3 System Get Ready 4 System Clear Errors 5 Autosampler Needle Cleaning 6 DA Det Balance 7 More Exit or Esc exits th
9. and the samples and standards are analyzed using a method called METH1 The example assumes that the sequence table is empty If the sequence table is not empty use the Delete button to delete all lines or use File New 1 2 3 4 Select the Sequence screen Move to line 1 and press Insert Select Method and then move to METHI and press Load Move to the End of sequence list select the Wizard and enter Vial Range 10 to 24 lnj 2 Agilent Instant Pilot Users s Guide 129 4 Running Multiple Vial Analyses Multiple Level Calibration Sequences 5 Select Calibration to display the Calibration Settings screen 130 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 Multiple Level Calibration Sequences 6 Change the settings according to Figure 85 Wizard Calibration V Use Calibration Calibration From 90 Multi ik To 92 Num lnj 2 m Before Xx m Every 5 Samples cea M After A Done A 11 51 Samples Calibration Preview f o Figure 87 Sequence Calibration Wizard 7 Press Preview to view the result Wizard Preview Num of Injections _ Cancel 11 51 Samples Calibration Preview Figure 88 Sequence Calibration Wizard Preview 23 2 2 2 2 2 2 2 vao 2 2 2 2 2 2 2 2 8 Select Done to accept entries 9 Press Start and confirm the start of the sequence Ag
10. e type B samples in vials 16 17 and 18 e three type B calibration standards in duplicate e type B samples in vials 19 20 and 21 and e three type B calibration standards in duplicate 136 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 Synchronizing Analyses with External Devices With an APG remote connector the system can be connected to external devices in order to synchronize the analyses This is necessary when an external device needs some time in order to get ready for a new analysis and when transmission of a start request is required see Connecting External Devices on page 107 for further information on interfaces The synchronization mode can be chosen in the Configure System screen g Configure System L Date 19 JAN 2006 Auto turn on OFF Turn off on error No Y Exit Allows you to set up external synchronization iS l 14 21 System I Controller ouat Pump autosampler vo Figure 91 Choosing the Synchronization mode In any case do all the analysis preparation using the Instant Pilot Agilent Instant Pilot Users s Guide 137 4 Running Multiple Vial Analyses A Start command is used to start the analytical run from the point of injection and is usually issued by the autosampler A Start Request command causes the autosampler to take the next vial and place it under the injection needle see APG Remote Enhanced Remote Infini
11. gt Continues will start all activities Cont automatically to get the system ready COO a Start Analysis Get Ready Interactive Action by user required Automatic Press continue to perfom actions Temporary Transient not ready condition Quat Pump Wait initialization DA Det Wait until lamp is on Current actions are displayed A Figure 73 Sequence Get Ready screen When all Get Ready activities are complete e the system status changes to green e the sequence starts immediately and e the last screen shows up Agilent Instant Pilot Users s Guide 99 2 Working with the Instant Pilot Sequence Automating Analyses Press Status to display the actual system status Idle Ce System min Controller aay Quaternary Pump Stoptime Current Method M1 Flow 0 000 m B OFF m 0 000 C OFF TCC C Controller Temp Een jae ead 0 4 bar Sequence status OFF vrni 0 0 a100 0 gt Autosampler 7 Diode Array Detector SL Speed hl min B BW A BW Inj Vol 5 00 pl Draw 100 sig 254 4 2547 16 nm Eject 100 m Use Ref Use Ref Ref 360 100 360 100 nm Vial ul 0 365 0 364 mau Valid from 0 00 to 99999min or OFF mR 16 38 Plot Setup J Select A Control Exit Figure 74 Sequence Status When you press Stop the Stop Analysis dialog pops up where you can select between aborting immediately or pausin
12. please contact your local Agilent support provider for help 162 Agilent Instant Pilot Users s Guide Maintenance and Repair 5 Repairing the Instant Pilot The Instant Pilot cannot be repaired except for the CAN cable Parts Identification Table 14 Part Numbers Description Part Number Instant Pilot G4208A complete assembly G4208 67001 CAN Cable G4208 81600 Upgrade Kit for NEW HOLDER for adapting the Instant Pilot to Agilent G4208 68701 1100 1200 series modules see Holder Versions for the Instant Pilot on page 19 Adapter Plate NEW for adapting the Instant Pilot to Agilent 1100 1200 G4208 60003 series modules see Holder Versions for the Instant Pilot on page 19 Adapter Kit Infinity Il for adapting the Instant Pilot to Agilent 1200 5067 5955 Infinity Il series modules see Holder Versions for the Instant Pilot on page 19 Includes holder and screw USB Flash Drive Kit G4208 68700 This product contains an TFT LCD assembly which is backlit by a mercury fluorescent lamp which contains mercury and must be managed recycled and or disposed in accordance with all applicable laws ordinances and regulations For information on how to recycle or dispose of the mercury lamp contained in this product or if you have additional questions on the mercury contained within this product contact Agilent customer service Agilent Instant Pilot Users s Guide 163 5 Maintenance and Repair Repairing
13. stop signal 108 system activities control 39 system information 37 system name 33 T temperature format 33 time amp date 32 current on display 31 format 33 on display 26 setting 32 time axis 103 time table method 39 timetable method 76 toggle 38 39 74 trace method changes 78 transfer file 83 transfer of methods 83 tray view 97 sequence 40 troubleshooting the Instant Pilot 161 USB Flash Drive not recognized 161 turning modules ON OFF 56 U units amp formats 32 unlocking Instant Pilot 24 update firmware 50 146 Agilent Instant Pilot Users s Guide Index Update Information for A 05 11 Firmware 153 USB flash drive unsupported formats 63 USB Flash Drive 13 icon 26 31 inserting 62 logbook 47 maximum size is 2 GB 62 161 maximum size is 2 GB 16 method 70 not recognized 161 odering information 163 saving method 81 saving screenshot 114 using 62 vendors 16 62 161 Ww weight and dimensions 17 wizard firmware update 50 sequence 40 setup 31 49 working with Instant Pilot 61 with methods 70 working with methods 70 X x axis 103 Y yaxis 103 177 Index 178 Agilent Instant Pilot Users s Guide www agilent com In This Guide This book provides information about the Agilent Instant Pilot e Start up Information e Working with the Instant Pilot e Running an Isocratic Analysis e Running Multiple Vial Analyses e Maintenance and Repair Ag
14. 06 01 12 Select Diode Array Detector DE03010634 i 1A 06 01 012 A 06 01 12 A 06 01 12 exists this screen A starts the update Update ee Allows you to select multiple modules for firmware update Ly 12 59 USB Flash Drive must be active Figure 100 Firmware Update Screen Update Wizard 3 Do your selections and press Update or Cancel to exit the screen Firmware Update Actions Ready to start Done G1311A uploading resident Done G1329A uploading resident G1 uploading residen G1311A activating resident G1329A activating resident G1316A activating resident G1315B activating resident G1311A uploading main G1329A uploading main G1316A uploading main G1315B uploading main 802 Figure 101 Firmware Update Screen Update in Progress In the case of an error refer to Errors During Firmware Updates on page 160 152 Agilent Instant Pilot Users s Guide Maintenance and Repair 5 Update Information for A 05 13 Firmware Do not downgrade to firmware revsions A 05 13 14 They are not compatible with G4208A Instant Pilots with serial MY Before you downgrade to revision A 05 15 upgrade the Instant Pilot to the latest B xx xx firmware revision This will assure that the later upgrade from A 05 15 to B xx xx works smoothly Using the Instant Pilot Downgrade to Revision A 05 15 In those cases where a Instant Pilot
15. 10 Select Method and then move to METH2 and press Load You have to insert METH2 METH3 before after each calibration sample line s Use copy paste or use insert parameter stoptime and only one method Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 11 Move to the End of sequence list select the Wizard and enter Vial Range 13 to 21 lnj 1 12 Select Calibration to display the Calibration Settings screen 13 Change the settings according to Figure 85 amp Wizard Calibration V Use Calibration Calibration From fa Multi Ir To 6 Num Inj 2 m Before gt 4 m Every 3 Samples caa C After A Done 15 16 Samples Calibration Preview Figure 90 Sequence Calibration Wizard 14 You may press Preview to view the result 15 Select Done to accept entries 16 Press Start and confirm the start of the sequence The autosampler now analyzes e three type A calibration standards in duplicate e type A samples in vials 7 and 8 e three type A calibration standards in duplicate e type A samples in vials 9 and 10 e three type A calibration standards in duplicate e type A samples in vials 11 to 12 e three type A calibration standards in duplicate Agilent Instant Pilot Users s Guide 135 4 Running Multiple Vial Analyses e three type B calibration standards in duplicate e type B samples in vials 13 14 and 15 e three type B calibration standards in duplicate
16. Agilent 162 Repairing the Instant Pilot 163 Parts Identification 163 Exchanging the CAN Cable 164 Appendix 167 Safety Information 168 Safety Symbols 168 General 169 Agilent Instant Pilot Users s Guide Contents Operation 169 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 170 Radio Interference 171 Test and Measurement 171 Agilent Technologies on Internet 172 Index 173 Agilent Instant Pilot Users s Guide 9 Contents 10 Agilent Instant Pilot Users s Guide G4208A Instant Pilot User s Guide 1 Start up Information Instant Pilot Features 12 Features and Benefits 14 Requirements for the Instant Pilot 15 Physical Specifications 17 Cleaning 18 Holder Versions for the Instant Pilot 19 Adding the Instant Pilot to an Agilent HPLC Module 20 Removing the Instant Pilot 24 Instant Pilot Display and Keyboard Layout 25 The i info key Online Information System 27 Basic Operational Concept of the Instant Pilot 30 Getting Started 31 System Information 37 Method Information 38 Sequence Information 40 Status Information 42 Logbook Information 47 Configuration 49 Maintenance Information 50 Early Maintenance Feedback EMF 53 Diagnosis Information 55 Turning Modules ON OFF Standby 56 Start Analysis Screen 57 Switching from G1323A B Control Module to Instant Pilot 58 Information on Firmware 60 This chapter provides general information around the Agilent Instant Pi
17. Agilent HPLC module the provided adapter plate is required Agilent Instant Pilot Users s Guide Start up Informatior Adding the Instant Pilot to an Agilent HPLC Module 1100 1200 1200 Infinity Series 1 Slide the adapter plate delivered with the Instant Pilot 2 Assure that it is fixed by pressing onto the adapter plate from the front onto the top cover of the Agilent HPLC module 3 Insert the Instant Pilot into the holder 4 Insert the Instant Pilot into the holder 5 The Instant Pilot in it s final position 6 Connect the CAN cable of the Instant Pilot into a free CAN connector at the rear of the module t Pilot Users s Guide 21 1 Start up Information Adding the Instant Pilot to an Agilent HPLC Module 1200 Infinity Il Series Content of Holder Infinity II Kit The kit 5067 5955 part of the Instant Pilot G4208A from May 2015 includes e Holder Infinity II e Screw e Installation Note Procedure Refer to the User Manual of your Infinity II module 1 Remove the right door of module 2 Remove the top leak panel 3 The holder must be clipped into the top cover 4 Insert the nose in the bottom of the cover into the slit in the holder and fit the holder on the cover 22 Agilent Instant Pilot Users s Guide Start up Information 1 Adding the Instant Pilot to an Agilent HPLC Module 5 Fix the holder with the screw 6 Re install the Leak Interface Top and
18. From 90 Multi ik To 90 Num Inj 1 m Before Xx m Every 2 Samples cea M After A Done A 11 24 Samples Calibration Preview o o Figure 85 Sequence Calibration Wizard 7 Press Preview to view the result Wizard Preview Num of Injections Cancel v9 v0 A x Done 11 22 Samples Calibration Preview Figure 86 Sequence Calibration Wizard Preview 2 2 1 2 2 1 v5 2 2 1 2 2 1 2 1 8 Select Done to accept entries 9 Press Start and confirm the start of the sequence 128 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 Multiple Level Calibration Sequences The following sections describe how to set up calibration sequences for analyses which use multiple level calibration Re calibrating With the Same Group of Standards There are three calibration standards of different concentrations Cl C2 C3 and 15 samples S The standards and samples are analyzed using the same method The analysis requires that each sample is analyzed twice the calibration standards are analyzed twice before the samples and re analyzed twice after every 5 samples C1 C2 C3 10 S14 C1 C2 C3 15 S19 C1 C2 C3 20 S24 C1 C2 C3 the calibration standards are located in positions 90 to 92 of the autosampler tray the 15 sample vials are located in positions 10 to 24 of the autosampler tray
19. It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated condi tions are fully understood and met Agilent Instant Pilot Users s Guide In This Guide This book describes how to operate the Agilent 1100 1200 1260 1290 Series modules and systems for HPLC using the handheld control module Agilent Instant Pilot G4208A 0 Figure 1 The Agilent Instant Pilot The Instant Pilot provides complete local control and monitoring of a single module or an entire Agilent 1100 1200 1260 and 1290 Series HPLC system There is no data evaluation in the Instant Pilot The Instant Pilot allows you to do a variety of HPLC tasks including automated sample preparation and injection isocratic gradient and multiple method analyses and basic diagnostics and maintenance If additional details are required on a specific topic function parameter not mentioned in this document please use the Instant Pilot s Online Information System i see The i info key Online Information System on page 27 Agilent Instant Pilot Users s Guide 3 Part 1 Part 2 Part 3 Chapter Overview Using the Agilent Instant Pilot This part describes the Agilent Instant Pilot its features and its functionality Start up Information This chapter provides general information arou
20. Module to a Single Agilent Module 20 the Control Module to an Agilent system 19 20 Agilent report problems 162 Agilent ChemStation 107 features 111 restricions 111 Agilent on internet 172 alphanumeric keys 26 analysis automated 91 isocratic 115 multiple vial 123 analysis start screen 57 APG Remote interface 107 power on 107 prepare 108 ready 108 shut down 107 start 108 start request 108 stop 108 asterisk sign Agilent Instant Pilot Users s Guide method 73 available signals 102 bar 33 BCD interface 107 board ID 37 brightness display 34 C cable 163 calibration 92 127 129 multiple level 129 sequence 127 129 settings 92 single level 127 CAN amp LAN connectors 20 cable replacement 164 Celsius 33 change product amp serial number 54 changes tracing 78 chromatogram 120 coexecution with 3rd Party Control Software 113 coexecution with Agilent ChemStation 111 column 116 column compartment 116 compare methods 39 compare methods 75 compatibility 15 concept of operation 30 configuration 49 Index connectors CAN amp LAN 20 contact agilent 162 control system activities 39 creating a sequence 119 current time 31 D date amp time 32 format 33 setting 32 details configuration 49 logbook 47 maintenance 50 method 38 sequence 40 status 42 system 37 diagnosis information 55 tests 55 with passed failed information 14 dimensions and weight 17 direction keys 26 display 101 brig
21. Press Print DIAGRES MHT Micro Pump Micro Press Test Print DIAGRES MHT Leak Tests Print DIAGRES MHT ISO Pump Bin Pump Quad Print DIAGRES MHT Preparation is described in Help Preparation Press Micro Pump Nano Pump Prep Pump steps like purging the pump are NOT included in the automatic actions list itself 68 If reports of the same type generated the files are named DIAGRES MHT DIAGR 1 MHT DIAGR 2 MHT and so on DOS 8 character naming convention Can be renamed For saving printing of screen shots refer to Saving a Screenshot to USB Flash Drive on page 114 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Printing To USB Flash Drive i Agilent Technologies SYSTEMI 08 25 06 10 02 system name date Holmium Test name of test plot Diode Array Detector SL PP00000024 module serial number plot 2001 gt 360 380 400 420 440 460 480 500 520 nm Action Result I Press continue to start Done Wait until lamp s ON Done actions Perform holmium test Done WL 1 360 0nm 362 0nm measured 360 8 Passed WL 2 452 7nm 454 7nm measured 453 0 Passed results WL 3 535 7nm 537 7nm measured 536 4 Passed Finished Passed Executed by sign off Date Signature Figure 42 Example of a Printed Document DAD Holmium Test Agilent Instant Pilot Users s Guide 69 2 Working with the Instant Pilot Working with Methods If add
22. Running Multiple Vial Analyses Analyzing Multiple Vials Using the Same Method 124 Analyzing Multiple Vials Using Different Methods 125 Single Level Calibration Sequences 127 Multiple Level Calibration Sequences 129 Re calibrating With the Same Group of Standards 129 Re calibrating With Multiple Groups of Standards 133 Synchronizing Analyses with External Devices 137 This chapter describes how to setup multiple vial analyses using the same method and different methods a Agilent Technologies 123 4 Running Multiple Vial Analyses Analyzing Multiple Vials Using the Same Method This section describes how to set up a 25 vial analysis with one injection from each vial You will use a previously created method The samples are located in positions 1 to 25 of the autosampler tray For details see Working with Methods on page 70 1 Enter the Sequence screen 2 Enter Wizard 3 Enter first 4 Enter last If the current method is used loading is not required 124 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 Analyzing Multiple Vials Using Different Methods This section describes how to set up a 50 vial analysis using three methods which you have previously created called e g METH1 METH2 and METHS For example METH1 and METH2 have the same analytical settings but differ in the injection volume and stoptime values METH3 uses a different temperature and requires a wait time of 30 minutes f
23. Setup screen S Sequence Displays the Sequence Setup screen Status Displays the Status screen Logbook Displays the Logbook m More Displays a menu that allows you to access the following items Content Figure 6 Online Information System Entry Screen closes the selected topic one step up in hierarchy opens the selected topic next sub topic one step down in hierarchy navigates to the previous sub topic exits the online help On the following screen different views are shown within the Online Information System Agilent Instant Pilot Users s Guide 27 1 Start up Information The i info key Online Information System Index Content Setting up a method Setting up a method timetable up a sequence Using inline editing Using the Sequence Wizard Running an injector program amp Working with Files Inline Editing Renaming Methods Using the Keyboard Setting up the Status Screen Using the Plot Screen Setting up the LAN Reference Concepts Error Messages Troubleshooting ong og oct o Figure 7 Online Information System Content left Home right Specifies at which points spectra are acquired and stored Multi Excitation must be enabled in order to acquire and store spectra None No spectra are taken Apex A spectrum is acquired at the apex of the peak Index ABCDEFGHIKLMNOPQRSTUVWYZ A Allin Peak All spectra within the peak are acquire
24. Table 4 26 Start up Information Instant Pilot Display and Keyboard Layout Item Key Group Description 1 Action keys Navigation keys Direction keys gt MY Esc key OK key or 8 i info key LED Numeric keys Alphanumeric keys Start Stop keys On line information USB icon Time trigger a variety of functions The available functions depend on the screen you are working with allow you to switch between the dialogs Within these dialogs the relevant parameters can be set and certain functions can be accessed The Navigation keys always correspond to a button displayed above them on the screen The dialogs accessed via the buttons vary according to the screen you are working with In some cases pressing a button causes a popup menu to appear From there you must make a choice in order to proceed you can move back left arrow and forward right arrow between the entry fields and up and down and scroll in lists allows you to exit the current window or screen and leads you back to the last window or screen you were working with In an edit field the previous value can be restored by pressing the Esc key you accept a current entry or action When entering a parameter into a certain field the OK key leads you on to the next accessible entry field In this case it has the same function as the right Direction key provides context specific information for current item selected st
25. describes the operation of the Instant Pilot Agilent Technologies 61 2 Working with the Instant Pilot Using a USB Flash Drive 62 You can use many USB Flash Drive with USB 1 1 support that can be physically inserted while the Instant Pilot is attached to the Agilent system Since USB Flash Drives may vary from vendor to vendor or from type to type incompatibilities can occur In general USB Flash Drives from Sandisk and Kingston should work The USB Flash Drives must be FAT 16 formatted and without encryption See USB Flash Drive Kit on page 163 See also Handling of Unsupported USB Flash Drive Formats on page 63 1 Open the USB cover 2 Insert the USB Flash Drive The display shows whether the USB Flash Drive is inserted and active by an icon not present grey present blue active yellow with red dot do not unplug Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Using a USB Flash Drive Figure 38 Inserting a USB Flash Drive Handling of Unsupported USB Flash Drive Formats If a unsupported format on a newly inserted USB Flash Drive is found the Instant Pilot brings up a warning and asks the user to format the drive in a proper way Unsupported Format Ea Unsupported USB Drive Format The plugged in USB drive uses an unsupported format Format the USB drive to use it with Ne Instant Pilot and loose all information da stored on it ves
26. done via CAN MIO LAN This interface enables the Agilent Series modules to communicate with PCs configured as CDS using a local area network LAN You can use the MIO interface if the respective extension board G1369C LAN Interface Card is installed in one of your modules detector is recommended or has on board LAN and your system is integrated in a LAN USB This interface has been introduced with the Infinity II Fusion modules and replaced the RS 232 interface The USB might be used for the Agilent Lab Advisor diagnostics and firmware updates and specific modules function e g dongle for HDR Serial RS 232 The module communication is enabled with the CAN cables This interface is also used by 3rd party control software and can be used for firmware upgrades Agilent Instant Pilot Users s Guide 109 2 Working with the Instant Pilot GPIB With the GPIB interface included in some modules your system is able to communicate with a Personal Computer configured as the Agilent ChemStation Connect all HPLC modules with CAN cables and use one of them to connect to the Agilent ChemStation via an GPIB cable GPIB was removed with the introduction 1200 Infinity modules 110 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Simultaneous Execution with Software With Agilent ChemStation Features All user interfaces the Instant Pilot the Agilent ChemStation or the Agilent Cerity WorkStatio
27. of all modules in the system with their available tests To select a test scroll down to the list and press Exec or OK to start the test A test report is shown at the end of the test Press Exit or Esc to leave the screen Agilent Instant Pilot Users s Guide 55 1 Start up Information Turning Modules ON OFF Standby Turning Modules ON OFF Standby 56 To switch a module ON or OFF or into STANDBY press Control from the Welcome Method Status Logbook screen System On Off Turns the pumps ON Turns the heaters ON Turns the lamps ON e MIS DAD UV IGNITION Turns all ON z e 3 Leaves this screen e Displays a table of the modules and their current states Ly 17 24 _BinPump tcc pad Jt Module specific tasks e g different lamps of a detector Figure 36 System On Off screen The three on off states off init ignition and on are grouped vertically to have a fast overview of the system s on off state The modules are grouped by modules types Pumps TCC temperatures and Detectors lamps with a frame next to the buttons on the right side The correlation to the buttons on the right side gets only lost if pumps or temps group has more then 2 modules Then the following groups are shifted downwards to free up the required space For high numbers of modules the overall modules list gets a scroll bar Agilent Instant Pilot Users s Guide Start up Informat
28. the displayed Resident Revison _A 06 02 001 Print information to the USB On time 66d 16 52h i Board ID TYPE G1311 66530 REV A4226 SER Flash Drive Autosampler DE91603245 Main Revision A 06 04 003 Resident Revison A 06 02 001 On time 42d 23 03h Board ID TYPE G1329 66500 REV B3844 SER 123 DE14923865 Main Revision A 06 04 001 Resident Revison _A 06 02 001 leaves the screen On time 64d 00 48h Board ID TYPE G1316 66520 REV C3918 SER v Exit Information on each module KS 10 48 Figure 17 Getting Started System Infuse The screen contains information about serial numbers and firmware revisions On time and Board ID of the modules Some modules may show additional information e g the G1316A Column Compartment shows an installed column switching valve or a module with installed LAN interface card shows the LAN address Depending on the number of modules you may have to scroll through the display Using the Print button all displayed information is saved to the USB Flash Drive into folder PRINTOUT as SYSINFO MHT Agilent Instant Pilot Users s Guide 37 1 Start up Information Method Information 38 To view edit the method information press the Method button from the Welcome screen w Method UNNAMED Posttime Quaternary Pump DE23923124 Stoptime 20 00 min Posttime OFF Flow 0 000 ml min B C D OFF OFF OFF Min Pressure OFF Max Pressure 400 bar Minim
29. the door Z Z 7 Insert the Instant Pilot into the holder 8 Connect the CAN cable of the Instant Pilot into a free CAN connector at the rear of the module Agilent Instant Pilot Users s Guide 23 1 Start up Information Removing the Instant Pilot Removing the Instant Pilot To remove the Instant Pilot slightly angle the Instant Pilot and then lift it from the holder Figure 4 Removing the Instant Pilot 24 Agilent Instant Pilot Users s Guide Instant Pilot Display and Keyboard Layout Start up Information Figure 5 shows the layout of the display and the keys All has been arranged in functional groups around the display a Agilent Technologies a E SEs 1260 Infinity G1315C DEX9077000 x FlexCube 1290 Infinity sr G4227A LP00000009 cai 2 UIB eee ar G1390A DE14300145 Display 8 9 Select a button to continue fe 12 23 Method J Sequence Status Logbook Morea Welcome A 1290 Infinity _G4226A LP00055026 TCC G1316C LP00000015 DAD SL 1290 Infinity a y Figure 5 The Agilent Series Instant Pilot Layout The displayed module s naming may change depending on the installed firmware and the connected modules 1100 1200 1260 1290 Agilent Instant Pilot Users s Guide 1
30. to other controllers To change the setting open the Configure Controller screen Scroll to 3rd Party Software and change to ON Backward compatible OFF visible to other controllers default Backward compatible ON invisible to other controllers A Configure Controller Fahrenheit PSI Month Day Year 24h Mode No Shutoff Temperature Unit Pressure Unit Date Format Time Format Backlight Shutoff Brightness 3rd Party Software Cancel 1804 Figure 83 Configure Controller 3rd Party Software Compatibility Whenever this setting is changed the Instant Pilot needs to be rebooted to make the change active If the Instant Pilot is in Backward compatible mode ON see With 3rd Party Control Software on page 113 it will not be seen by the LAN RS 232 Firmware Update Tool For updating firmware reconfigure the Instant Pilot to Backward compatible mode OFF Agilent Instant Pilot Users s Guide 113 2 Working with the Instant Pilot Special Functions Saving a Screenshot to USB Flash Drive You may want to create a screenshot for e adding it to a documentation or e troubleshooting reasons To do so e insert the USB Flash Drive e wait until the USB Flash Drive has been initiated e press the key sequence i dot info The screenshot is saved as SCR nn BMP where nn is a number on the USB Flash Drive The figure can be opened on the PC with any graphic or word proce
31. 3 or 4 Agilent Instant Pilot Users s Guide 101 2 102 Working with the Instant Pilot Setup of Signals Up to four of the available signals can be chosen for graphical display 1 From the Plot screen press Setup to show the Selection screen 2 Use the Direction and Selection keys to navigate within and between the available signals and selected signals list boxes E Status Plot Signals Name YMin Y Max z Temp Right 10 00 80 00 PC al T ggle Variable WL Detector SL Signal 10 00 1000 mAU Diode Array Detector SL Clear Signal A 1 68 42 08 mAU Signal B 12 45 90 05 mAU E Signal C 10 00 1000 mAU ES Signal D 10 00 1000 mAU Edit Signal E 10 00 1000 mAU Signal F 10 00 1000 mAU x Signal G 10 00 1000 mAU Cancel Signal H 10 00 1000 mAU Fluorescence Detector xj v Time Range 10 00 min Done Allows you to edit the y axis range of the signal KS 15 14 Figure 77 Plot screen Setup signals Selects a signals Clears all selections Edit the settings for scaling Cancels the actions and leaves the screen Accepts the settings and switch to graphic view On the right side of the Selected Signals list box you can see the legend to the signals The different signals can be selected by pressing Toggle or OK Depending on which signal is highlighted you can enter an individual Y Range setting here using Edit 3 When the signals an
32. 311A DE23923124 A 06 03 001 Autosampler G1329A DE91603245 A 06 02 002 H TEC G1316A_ DE14923865_ A 06 03 001 4 Update Wizard allows VWD SL G1314C JP92110261 _A 06 03 001 ot selective updates DAD SL G1315C PP00000024 B 01 03 0001 Ee FLD G1321A DE92001563 A 06 02 001 PN SN a Exit Displays information on the available modules ks l 11 26 USB Flash Drive must be System I Controller ouat Pump Attosampier vo active Figure 99 Firmware Update Screen Update Wizard The Update Wizard allows you to define the modules to be updated This screen also shows the installed firmware revision versus the available 1 Press Update Wizard and then Cont or Abortto cancel the update process 2 The next screen Figure 100 shows all modules their installed firmware revisions and the available firmware revisions on the USB Flash Drive Agilent Instant Pilot Users s Guide 151 J 5 Maintenance and Repair Jp i Maintenance Firmware Update Wizard I Current FW Newest main Newest resident z select de select a module Controller DE6055502 Toggle B 02 01 0001 ie 3 Quaternary Pump DE23923124 clear all selections A 06 01 012 A 06 01 12 A 06 01 12 a Autosampler DE91603245 gar A 06 01 012 A 06 01 12 A 06 01 12 elect all module Column Compartment DE14923865 a select all modules A 06 01 012 JA 06 01 12 A
33. Agilent Instant Pilot Users s Guide 119 3 Running an Isocratic Analysis Observing the Chromatogram Aa Q N 6 Select the Status screen Press Start Select Continue to confirm the start of the analysis If the system is not ready yellow you have to press Continue again The modules automatically get ready and start the analysis Change to the Status screen if started from here the plot will be active after start Press Plot to show the chromatogram A typical chromatogram for this analysis is shown in Figure 84 mAU 350 4 300 4 250 4 200 4 150 100 A fl 1 2 3 4 5 6 min Figure 84 Analysis of lsocratic Standard Sample 120 Agilent Instant Pilot Users s Guide Running an Isocratic Analysis 3 The exact profile of the chromatogram depends on the column you have used Differences in retention times and areas of the peaks in your chromatogram and the one shown in Figure 84 might be a result of variations in the concentration of the sample from batch to batch the quality of the solvents used and the column temperature You can rescale the plot using Rescale or the cursor keys or you define the plot window within the Setup see also Rescaling the Plot Screen on page 103 Agilent Instant Pilot Users s Guide 121 3 Running an Isocratic Analysis Observing the Chromatogram 122 Agilent Instant Pilot Users s Guide G4208A Instant Pilot User s Guide 4
34. Figure 39 Unsupported USB Flash Drive When selecting No theUSB Flash Drive will be ignored can not be used in the Instant Pilot even it is still inserted Agilent Instant Pilot Users s Guide 63 2 64 Working with the Instant Pilot When selecting Yes there are two possible responses formatting succeeds or fails During the format of the USB Flash Drive all stored data currently will be lost USB Drive USB Drive mes Drive Formatted Successfully x Failed to Format Drive The USB drive was formatted with a The plugged in USB drive cannot be supported format and can now be formatted to be used with Instant used with Instant Pilot Pilot d4 Please try another type of USB drive JV Ok Ok Figure 40 Format of USB Flash Drive succeeded or failed In case it failed try a different type of USB Flash Drive or use the Agilent recommended USB Flash Drive Kit on page 163 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Printing To USB Flash Drive There is no direct printing via a printing device connected to the 1100 1200 1260 1290 system possible But certain information can be printed to a file that is saved to an USB Flash Drive into a folder PRINTOUT The files are of type MHT or HTM depending on the setting in Configuration Controller Print document as The difference is MHT all files of a printout are in a single archive file preferred HTM a htm file pl
35. Pilot to Backward compatible mode OFF If your Agilent 1100 1200 system runs on firmware A 05 xx the Instant Pilot is not recognized by the LAN RS 232 Update Tool 2 10 In this case either one of the modules or the complete system must be upgraded to A 06 xx B 0x xx Before you downgrade the Instant Pilot to revision A 05 15 upgrade the Instant Pilot to the latest B xx xx firmware revision This will assure that the later upgrade to B xx xx works smoothly The preferred way of updating the Instant Pilot firmware should be the use of a USB Flash Drive This is much faster than the use of the LAN RS 232 Update Tool 2 10 4 minutes vs 10 minutes via LAN vs 70 minutes via RS 232 Use one of the following procedures e Downgrade from B xx to A 05 1x on page 157 e Upgrade from A 05 1x to B xx on page 157 e Upgrade from A 05 11 to A 05 13 on page 158 Agilent Instant Pilot Users s Guide Maintenance and Repair 5 Downgrade from B xx to A 05 1x 1 When the Agilent 1100 1200 series modules are on a firmware platform A 06 xx downgrade the Instant Pilot to A 05 1x first During the downgrade process the screen is black 2 After boot of the Instant Pilot the Instant Pilot shows Scanning System the LAN RS 232 Update Tool 2 10 indicates 100 updated but does not display the result dialog 3 Press Cancel red cross button on the LAN RS 232 Update Tool 2 10 to stop the Update process The Instant Pilo
36. Ref V Use Ref Wi Ref 360 100 360 100 nm Done yial a il ul 65 53 42 08 mAU Allows you to set up the status screen 11 46 valid from 0 00 to 99999min or OFF J 11 47 Defaut Properties File Pit Setup Selecta Control Exit Figure 23 Status Screen Selection vs Displayed Agilent Instant Pilot Users s Guide 45 1 Start up Information Status Information Press Properties on the Setup screen to access the history of the current status view changes and the protection of the status view Status Setup Properties Name A Description i comment Created 02 09 07 a protect unprotect a status chaned Sonia 02 09 07 16 42 07 ae meine passat Changed content 02 20 07 15 29 37 leaves this screen without changes select a signal parameter Displays the properties of an object 16 02 Figure 24 Status Screen Properties History Status Setup Properties Name WA 1 Description f comment Changed content protect unprotect a status view Disp Enter Password enter a password Cancel Reenter Password Figure 25 Status Screen Protection 46 Agilent Instant Pilot Users s Guide Start up Information 1 Logbook Information To view change the Logbook information press Logbook from the Welcome screen g Logbook System L ii to define what is displayed Message Time a A i Temperature off 13 28 52 a
37. Welcome screen 11 Close the connection and the LAN RS 232 Update Tool 2 10 Agilent Instant Pilot Users s Guide 159 5 160 Maintenance and Repair Firmware Updates Errors During Firmware Updates If an error stops the update process it is displayed Actions Firmware Update Ready to start Done G1311A uploading resident Done G1329A uploading resident Done G1316A uploading resident Done G1315B uploading resident Done G1311A activating resident Done G1329A activating resident Done G1316A activating resident Done G1315B activating resident timeout Error G1311A uploading main G1329A uploading main G1316A uploading main G1315B __ 7 8G uploading main Abort a See Sa Figure 107 Firmware Update Screen Error during the update Press Abort to leave the Update process In the above example all modules stay in resident mode blinking yellow Restart the firmware update again Agilent Instant Pilot Users s Guide Maintenance and Repair 5 Troubleshooting Internal diagnostics continuously monitor the module s condition and record any unusual events in an electronic logbook see Logbook Information on page 47 For example missing vials or leaking solvent errors and record the errors in the logbook together with the time and date of the occurrence The logbook is self updating Al
38. aaa Fie File opens the file operations Tray View shows the Properties opens the Wizard opens the current sequence s history protectionof wizard to set up a status graphically the current sequence sequence Figure 63 Sequence Start up screen Using the Sequence screen you can link several methods together For example you can first run a method containing an injector program to do sample preparation followed by an analytical run to analyze a batch of samples You can then run a second method to analyze further samples with different analytical conditions Agilent Instant Pilot Users s Guide 91 2 92 Working with the Instant Pilot When the second method is loaded it waits for a specified time before starting the analysis allowing the column to equilibrate to the new conditions All sequence events can be traced in the Logbook available from the Welcome screen ra Sequence UNNAMED Started Num of Injections End of Sequence Location Insert a line for details refer to Instant Pilot s Info System 1 Sample 2 Method 3 Parameter 4 Wait time 1 Blank Run 2 Wait until 3 Not ready timeout 4 Stabilization 5 End Actions m Idle oho Exit 16 17 Tray View Properties Wizard File Figure 64 Sequence Add a sequence line At the end of the sequence you can specify either to load a method e g to flush the LC system to remove buffer sa
39. able Analysis screen Sequence Analysis screen Vial range Analysis screen ON OFF on various screens System screen System screen Control System screen Configuration System screen Tests System screen Records System screen Records EMF System screen Records Logbooks Welcome screen Status Welcome screen Method Welcome screen Method Time Table Welcome screen Sequence Start button Sample Range Control button System ON OFF and System Get Ready on various screens Welcome screen Logbook Control button on various screens Welcome screen More Configuration Welcome screen More Maintenance Diagnostics Welcome screen Details System Info Product number serial number board ID and firmware revision Welcome screen More Maintenance System Welcome screen More Maintenance Module EMF Setup Welcome screen Logbook System Controller Modules Welcome screen More Maintenance EMF Events Errors Events Module and Maintenance Entries 58 Agilent Instant Pilot Users s Guide Start up Information 1 Table 10 G1323A B Control Module vs Instant Pilot Functions G1323A B Control Module G4208A Instant Pilot Comment System screen Records FW Update Welcome screen More Maintenance System Firmware updates and Single Wizard PN SN Product and Serial Number change Status screen Welcome screen Status Plot screen Welcome screen Sta
40. ade bidistilled water 35 and acetonitrile 65 For the binary or quaternary pump fill one solvent bottle with bidistilled water channel A and the other with acetonitrile channel B 2 Turn on the detector lamp and pump via the Control System On OFF screen 3 For the quaternary pump turn on the degasser by pressing the line power switch 4 Purge the pump Allow the detector at least 15 minutes to provide a stable baseline Transfer the contents of an Agilent Technologies isocratic standard sample ampoule into a vial and seal the vial with a cap Place the vial in position 1 of the autosampler tray 7 Pump the water acetonitrile 35 65 mobile phase through the column for 10 minutes at a flow rate of 2 ml min Agilent Instant Pilot Users s Guide 117 3 Running an Isocratic Analysis Entering Settings To set up the isocratic analysis you will set the LC system settings to default and then modify selected settings The other settings will remain with their default values You will then save these settings to a method called ISO 1 2 3 4 5 Enter the Method screen Select Control and select System Set Defaults Set the Stoptime to 6 minutes Set the Flow to 1 5 ml min Set B 65 C OFF D OFF for quaternary pump If you have a binary pump set B to 65 Set Injection volume to 1 ul The channels of a pumping system are named A B C and D depending on the pump type A is automatically ca
41. all modules of the system in the Welcome screen Close the connection and the LAN RS 232 Update Tool 2 10 Upgrade from A 05 11 to A 05 13 If the Instant Pilot needs to be updated with a new firmware revision follow these steps 1 Disconnect all 1100 1200 series module from that module that hosts the LAN RS 232 interface Upgrade this module to firmware A 06 xx first otherwise the Instant Pilot is not visible in the LAN RS 232 Update Tool 2 10 3 After boot enter the Maintenance screen and press the Controller button 4 Press the Transfer button The Instant Pilot will switch into resident mode black screen and the Update Tool lists now the Instant Pilot Select the Instant Pilot for update to the target firmware revision Agilent Instant Pilot Users s Guide Maintenance and Repair 5 6 Start the update 7 After boot of the Instant Pilot the Instant Pilot shows Scanning System the LAN RS 232 Update Tool 2 10 indicates 100 updated but does not display the result dialog 8 Press Cancel red cross button on the LAN RS 232 Update Tool 2 10 to stop the Update process The Instant Pilot is no longer listed in the tree view of the Update Tool 9 Downgrade the 1100 1200 module to the target firmware A 05 11 13 or A 05 09 10 10 When finished the LAN RS 232 Update Tool 2 10 shows all 1100 1200 modules without the Instant Pilot the Instant Pilot shows all modules of the system in the
42. analyzed twice and re analyzed after every 3 samples C1 C2 C3 13 S15 C1 C2 C3 S 16 S18 C1 C2 C3 19 S821 C1 C2 C3 the calibration standards of type B are in positions 4 5 and 6 of the autosampler tray and the 9 sample vials are in positions 13 to 21 and the samples and calibration standards of type B use different methods The samples use METH2 and the calibration standards use METH3 These methods contain the same analytical parameters and differ only in the analysis stoptime Agilent Instant Pilot Users s Guide 133 4 134 Running Multiple Vial Analyses The example assumes that the sequence table is empty If the sequence table is not empty use the Delete button to delete all lines or use File New 1 Select the Sequence screen 2 Move to line 1 and press Insert 3 Select Method and then move to METHI and press Load 4 Move to the End of sequence list select the Wizard and enter Vial Range 7 to 12 lnj 1 5 Select Calibration to display the Calibration Settings screen o Change the settings according to Figure 85 Peal Wizard Calibration V Use Calibration Calibration From fi Multi a To 3 Num lnj 2 m Before x m Every 2 Samples Emea M After vi Done 12 56 Samples Calibration Preview Figure 89 Sequence Calibration Wizard 7 You may press Preview to view the result 8 Select Done to accept entries 9 Move to the End of sequence list and press Insert
43. aratus is marked with this symbol when the user should refer to the instruction manual in order to protect the apparatus against damage p Indicates dangerous voltages A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met 168 Agilent Instant Pilot Users s Guide General Appendix 6 This is a instrument has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Any maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation i
44. are Release Archive Release _A 06 50 B 06 50 Patches Change folder Update pending 1 Figure 104 Lab Advisor Select the firmware 5 The firmware will be updated and shows the beow screen when finished Instrument Name Progress Caution During the firmware update procedure do not tum instrument off nor disconnect AC power Doing so may result in the instrument becoming unresponsive Te Lock Device Resident Firmware Main Firmware Progress G1321A FLD A 06 50 003 A 06 55 001 Serial DEABO03324 E G4208A 1200 Instant Pilot B 02 16 0001 B 02 16 0001 S gaii f Serial DE13599999 Firmware files are currently taken from wadnas 1 WAD Fimware Public Fimware Release Archive Release _A 06 50 B 06 50 Patches Change folder Figure 105 Lab Advisor Updated finished 6 Close Lab Advisor Agilent Instant Pilot Users s Guide 155 5 156 Maintenance and Repair Using the LAN RS 232 Firmware Update Tool Detailed information about the LAN RS 232 Update Tool 2 10 or above can be found within the documentation of this tool provided via the Agilent web at http www chem agilent com _layouts agilent downloadFirmware aspx w hid 69761 Use the latest LAN RS 232 Update Tool 2 10 only If the Instant Pilot is in Backward compatible mode ON see With 3rd Party Control Software on page 113 it will not be seen by the Firmware Update tool For updating firmware reconfigure the Instant
45. assed failed result and do not modify anything on the instrument Maintenance Maintenance shows the logs for maintenance relevant events allows access to EMF early maintenance settings and functions needed for maintenance tasks e g calibration routines parts information Agilent Instant Pilot Users s Guide Start up Information 1 Getting Started If additional details are required on a specific topic function parameter not mentioned in this document please use the Instant Pilot s Online Information System i see The i info key Online Information System on page 27 Starting the Instant Pilot the first time displays the Setup Wizard shown in Figure 9 Status LED summarizes the status of all modules Setup Wizard It is important that all devices of the system are active before instrument setup is started Please check the list below to insure that all devices are active before you continue Quat Pump G1311A DE23923124 leaves the setup wizard Autosampler G1329A DE91603245 Abort Col Comp G1316A DE14923865 DA Det G1315B DE03010634 a gt to next screen Cont a Figure 9 Getting Started Setup Wizard The display shows the actual connected and powered up Agilent modules The color gives the current STATUS yellow not ready gray ready green run red error In the bottom right the current time and the USB Flash Drive icon is shown If a USB Flash Dri
46. ations Start up Information 1 Type Specification Comments Weight 0 8 kg 1 76 Ibs Dimensions 130 x 225 x 35 mm width x depth x height 5 1 x 8 9 x 1 4 inches Line voltage 22 VDC 10 via CAN Power consumption 6 W 20 5 BTU hour Maximum Ambient operating temperature Ambient non operating temperature Humidity Operating altitude Non operating altitude Safety standards IEC CSA UL EN 0 55 C 32 131 F 40 70 C 40 158 F lt 95 at 25 40 C 77 104 F Up to 2000 m 6562 ft Up to 4600 m 15092 ft Installation category Il pollution degree 2 For indoor use only Research Use Only Not for use in Diagnostic Procedures Non condensing For storing This product contains an TFT LCD assembly which is backlit by a mercury fluorescent lamp which contains mercury and must be managed recycled and or disposed in accordance with all applicable laws ordinances and regulations For information on how to recycle or dispose of the mercury lamp contained in this product or if you have additional questions on the mercury contained within this product contact Agilent customer service Agilent Instant Pilot Users s Guide 17 1 Start up Information Cleaning The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth allowing liquid to drip i
47. atus LED green if Instant Pilot is connected via CAN and has booted enter numbers 0 to 9 allow you to enter numeric values in parameter entry fields In certain fields where alphabetical characters may be entered you can use the Numeric Alphanumeric keys to do so Pressing them several times in sequence changes the current value e g 1 ABC 2DEF 3GH1 starts or stops running applications gives addition information about the topic that is selected shows whether the USB Flash Drive is inserted and active Not present grey present blue active yellow with red dot do not unplug displays the current time Agilent Instant Pilot Users s Guide The i info key Online Information System Start up Information 1 The online information system provides a quick and convenient way to look up information about a task you are doing or a feature or screen you would like to know more about The online information system is context sensitive and provides information related to the current topic You can access the online information system by using the i info key on the Instant Pilot s keyboard Welcome Select a button to continue lees Control System Info w Control Displays a menu that allows you to access the following items x System Set Defaults s e System On Off a e System Get Ready System Clear Errors Details Displays the System Info screen aad Method Displays the Method
48. ault amount from sample Draw pl from vial 1 End of User Defined Injector Program Cancel valid from 0 00 to 100 0p1 10 52 Figure 62 Injector Program Modifying a Parameter 88 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Table 12 lists all injector program lines that are insertable editable Table 12 Insertable Editable Injector Program Lines Command Comment Draw default amount from sample from actual position Draw default amount from sample plus x vial s from actual position Draw x ul from vial y Draw x ul from seat Draw x ul from air Draw x ul from flush DLA only Eject all into seat Eject x pl into sample Eject x pl into location y Eject x pl into seat Eject x pl into air Mix x pl in seat z time s Mix x pl in air z time s Mix x pl in air at y pl min at z time s Mix w ul in location x offset y z time s WPS only t Inject Valve mainpass Valve mainpass with start pulse Valve bypass Needle up Needle into seat Needle into vial x Needle to wash port Wash needle in default wash vial x time s Agilent Instant Pilot Users s Guide 89 2 Working with the Instant Pilot Table 12 Insertable Editable Injector Program Lines Command Comment Wash needle with default wash parameters Wash in vial x y time s Wash in flush port for x sec WPS only Move vial from sample position to waste location 220 ALS only Remote ready R
49. ay Year Day Month Year Pressure Bar H Bi Bar PSI kPa Temp Celsius Ea Celsius Fahrenheit Kelvin ub Next l 16 13 Figure 12 Getting Started Setup Wizard Units amp Formats Agilent Instant Pilot Users s Guide 33 1 34 Start up Information Setup Wizard Display It is recommended to define a time after which the backlight is shutoff to prevent lamps from exceeding their lifetime After 1 10 30 60 Display minutes No shutoff Shutoff time Xia aa Brightness fi 00 M qu Back Settings for prolonging the life of your display Ly 16 14 10 20 30 40 50 60 70 80 90 100 Figure 13 Getting Started Setup Wizard Display The next screen shows the LAN settings of an additional MIO card that has been identified in the system may not been connected to LAN Setup Wizard LAN Settings i Diode Array Detector SL ee allows modifications of the Init Mode USING STORED Modify settings For parameters refer to the installed LAN LAN Settings IP Address 134 40 27 95 card s documentation Subnet Mask 255 255 248 0 Abort Def Gateway 134 40 24 1 a MAC Address 0030D30A0838 Figure 14 Getting Started Setup Wizard LAN settings The next screen shows the actual LAN settings used for communication with the system module Agilent Instant Pilot Users s Guide Setup Wizard LAN Settings LAN Setup i Settin Value
50. ce or see www agilent com for more information This product contains an TFT LCD assembly which is backlit by a mercury fluorescent lamp which contains mercury and must be managed recycled and or disposed in accordance with all applicable laws ordinances and regulations For information on how to recycle or dispose of the mercury lamp contained in this product or if you have additional questions on the mercury contained within this product contact Agilent customer service Agilent Instant Pilot Users s Guide Appendix 6 Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises Agilent Instant Pilot Users s Guide 171 6 Appendix Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Life Sciences amp Chemical Analysis Solutions 172 Agilent Instant Pilot Users s Guide Index Numerics 3rd Party Control Software coexecution 113 A A 05 11 firmware down upgrade 153 action keys 26 adapter plate 163 adding the Control
51. ctivity Maintenance Screen Select Maintenance Activity Agilent Instant Pilot Users s Guide Start up Information 1 Early Maintenance Feedback EMF In case you have set the EMF limits and the limit has been reached a message pops up Welcome EMF Counter Exceeded Ue EM Aut y UV lamp burn time limit exceeded The burn time limit for the UV lamp has exceeded its defined limit Check your part and or adjust your limit setting in Maintenance EMF Setup Figure 32 Early Maintenance Feedback EMF Message The limits can be set in the EMF Setup screen QV Maintenance EMF Setup VWD SL Burn time UV lamp actual changes the color depending on state green below limit yellow limit exceeded red far above limit Exit The total operation time of the UV lamp 15 49 Figure 33 Early Maintenance Feedback EMF Setting the limits Agilent Instant Pilot Users s Guide 53 1 54 Start up Information Product Number and Serial Number Change When the main board has to be replaced the new board does not have a serial number For some modules e g pumps or auto samplers the type has to be changed multiple usage boards Use the information from the serial number plate of your module The changes become active after the reboot of the module This function should be used by Agilent trained personnel only Otherwise the module may be no
52. ctory Agilent Instant Pilot Users s Guide Start up Information 1 On the module specific screens you can e see the Early Maintenance Feedback EMF error and maintenance events e set the EMF limits see Early Maintenance Feedback EMF on page 53 e do module maintenance e g calibrations e add maintenance activities into the permanent log e identify the module in the stack flashing LED Press the Exit button or Esc key to leave the screen 86 Maintenance Quat Pump ey EMF setup Setup oO select maintenance activity Entries Identify module LED blinks Ident J Exit OI Ij System I Controller Quat Pump Autosampier o Figure 30 Maintenance Screen Pump EMF Events Error Events Empty Maintenance Entries Pump seals replaced 111961 qqpurtra02 03 05 13 41 Pump head assembly replaced 63 609 22 02 05 16 29 Agilent Instant Pilot Users s Guide 51 1 52 Start up Information Maintenance Information Maintenance Quat Pump Maintenance Maintenance entries Pump seals replaced Pump pistons replaced Purge valve frit replaced Solvent inlet filter replaced x AIV replaced Cancel Outlet valve replaced Pump head assembly replaced Pump head assembly replaced System J Controller Quat Pump Autosampler Figure 31 select maintenance activity from list Saves the maintenance a
53. ctrum 0822 mAU 1233 0 rim i p i Single pe geene aee eee a Nw BB Starts continuous spectrum a S stops continuous spectrum Stop gt prints spectrum to USB Flash Vi Print Drive 200 220 240 260 280 300 320 340 360 380 nm G exists the screen o gt Scan from 190 nm tof 400 nm step 2 0 nm Exit Valid from 190 to 950nm 11 00 M Cursor Cursor Balance Peaks Figure 79 Spectrum Example DAD The scan range and step width can be set Using the Single button starts a single spectrum Using the Start Stop button starts stops a continues spectrum Using the Cursor buttons the cursor can be moved left or right on the wavelength axis Using the Balance button the detector performs a baseline balance Using the Peaks button a table with all found peaks is displayed also part of the printed spectrum Using the Blank button VWD only a blank background scan is taken Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 DAD MWD VWD FLD Spectrum FLD Spectrum Under Control More the user has two additional options Excitation Spectrum and Emission Spectrum Both screens are similar only the editable parameters are different according the selected spectra type see Figure 80 The screen has a Single scan mode and a Continues mode accessible via Start Peaks can be displayed see Figure 81 and the result can be printed Emission Sp
54. d All All spectra are acquired with the settings of the scan Spectral acquisition depends on the setting of the acquire spectra Acquire Ex Spectra FLD Acquire Em Spectra FLD AFC EMF Setup AGP remote AGP Remote ALS EMF Setup ALS Injector Steps ALS Maintenance ALS analog out Analog Out Source VWD Analog Out Polarity VWD analog output Analog Out 1 2 DAD and MWD Analog Out VWD Analog Attenuation RID Analog Voltage Range RID Analog 1 2 Source FLD archive Print documents as Aka ettne Annlan Ant 12 Abtamintian DAD anal BAIN Te SS a ESS STE Figure 8 Online Information System Index left Details right Peakwidth Eight spectra are acquired per Peakwidth The acquisition time for one spectrum is slightly less than the Peakwidth divided by 8 that is greater than or equal to 0 01 s and less than or equal to 2 55 s All w o signal All flashes are used for spectral acquisition When All wio signal is selected the mean value of all measured wavelengths is shown on channel A This setting is useful for unknown spectra DE The spectral acquisition modes Apex and All in Peak are gt oo ogo coc oog gt oo ogo coc oo 28 Agilent Instant Pilot Users s Guide Table 5 Start up Information 1 Online Help Functions of Keys Button Description OK key or 8 Esc or hor Content Home Index Back Forward navigates to the selected focussed l
55. d their Y signal unit ranges have been specified press Done to switch to the graphic view Agilent Instant Pilot Users s Guide Rescaling the Plot Screen Working with the Instant Pilot E Status Plot t Pressure SigA bar 3504 300 2504 200 1504 100 50 oL EA 252 25 2 Quat Pump Pressure 258 259 260 261 min 3 Signal A J 15 29 RescaleA Selecta E Figure 78 Plot screen Setup signals Press Rescale and select the signal X time axis To rescale the X time axis use the Direction keys left right Y signal unit axis There are several possibilities to rescale the Y signal unit axis e Via Setup you can specify a Y range separately for each signal Rescaling directly from the Plot screen overwrites these settings e Use Rescale in the Plot screen to adjust the Y axis according to the minimum and the maximum signal value within the set time range 2 Using this function provides the optimum signal display It refers to all or to a selected signal from the menu e Use the Direction keys up down to change the scaling of the Y axis by a factor of 2 up or 1 2 down of the selected signal Agilent Instant Pilot Users s Guide 103 2 Working with the Instant Pilot DAD MWD VWD FLD Spectrum 104 Via the Control button and More the DAD MWD VWD FLD spectrum screen is accessible 7 Spectrum DAD SL g Single spe
56. dit or OK 3 Enter the new value 4 Press Done w Method WOLF all Edit the selected parameter Edit Posttime OFF Opens a menu to control certain Quaternary Pump DE23923124 Control system settings activities Stoptime OFF Posttime OFF Flow 0 000 ml min B C D OFF OFF OFF Min Pressure OFF Max Pressure 400 bar Minimum Stroke 50 ul Compressibility 100 10E 6 bar Max Flow Gradient 100 0 ml min G Exits this screen Primary Channel Auto xj Exit The rate of movement of eluent along the column ky 15 06 Figure 45 Method Edit screen If you change a method setting the value is immediately downloaded to the LC module An asterisk will appear next to the method name to indicate that the current method has been modified An hash will appear next to the method name to indicate that the method is from a different configuration setup with other modules Modules marked red are missing or not switched on Agilent Instant Pilot Users s Guide 73 2 74 Working with the Instant Pilot Filtering Method Information When a Filter is selected only the parameters that are selected in this filter are shown on the Method screen w Method Filter z Selects de selects the Toggle parameter I Selects all parameter System lt Posttime Quaternary Pump Select Stoptime vY Posttime x Flow B C D cear Min Pre
57. e reset to defaults during update Continue with update 12 50 Figure 97 Firmware Update Screen Ready for Update 3 Press Cont button to confirm and Cont start the update or Cancel to exit this screen The actions and the progress is shown Firmware Update Actions 150 gt Ready to start Done G1316A uploading resident Done Update Informati a A ton G1316A activating resident Done p G1316A uploading main Done IG1316A activating main Done Finished Done When finished press Exit Update progress bar a LO O MaM Figure 98 Firmware Update Screen Update has completed In the case of an error refer to Errors During Firmware Updates on page 160 Agilent Instant Pilot Users s Guide Maintenance and Repair 5 Updating the Firmware Using The Wizard CAUTION Only one firmware revision set should be stored on the USB Flash Drive If more than one firmware version for a module is available the Instant Pilot will always take the most recent firmware version In this case delete those versions not required Do not disconnect the Instant Pilot or the USB Flash Drive while a firmware update is running Otherwise the module may become unusable To open the Maintenance System information press More from the Welcome screen and select Maintenance g Maintenance System Product Serial Firmware Controller ERRNO Single Quat Pump G1
58. e Status screen e gt moves the curser up or down to an editable field nv moves the curser up or down in a content list OK key or 8 edits the selected parameter Agilent Instant Pilot Users s Guide 43 Start up Information Setup of a Status Information Screen When the Status Information screen has not been setup before it will show from each module in the system one or more signals parameters default Press the Setup button Status Setup DEFAULT allows the selection of a Controller Quat Pump ztopime Current Method Analysis Select signal parameter es clears a selected field TCC Temp Left Clear Editable x cuts a selected field to be T sanpier DADISI Cut pasted to another position Analysis Analysis N leaves this screen without Cancel changes leaves this screen with all Done changes Allows you to set up the status screen ks l 71 46 File load save a setup Defaut Properties File Default default setup based Properties history and on system protection Figure 21 Status Screen Setup The status view setup shows tile types as editable and Analysis see Figure 22 Entering the setup select dialog now focuses the currently selected tile s entry in the list or if it is an empty tile the last selected entry In the selection list the tile types have the same naming as in the setup dialog editable to have a large or small ti
59. e ordered by module default or time Q Method Timetable E Edit a line End of Timetable 1 Quaternary Pump DE23923124 2 Column Compartment DE14923865 3 Diode Array Detector DE03010634 Insert a line with choice of module Copy a line Delete a line Saves the settings and leaves the screen fe 15 14 Sort lines by module or time Sort Module SortTime PE Figure 48 Method Timetable screen Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 A timetable line can be inserted by pressing Insert and consists of the following Time Set the time span between the instant of injection and the desired parameter change e Setting Select the parameter to be changed e Value Enter the desired parameter value You can edit an existing timetable line by pressing Edit or OK Use Delete to delete the selected line You can copy a timetable line by pressing Copy Q Method Timetable Edit a line and change the parameter Diode Array Detector DE03010634 Oy Signal A 3 250 100 Use Ref gt 360 100 nm End of Timetable x Cancels the action and leaves Cancel the screen Jf Saves the settings and leaves Done the screen Valid from 0 00 to 99999 00min Ry 15 14 Figure 49 Method Timetable screen Agilent Instant Pilot Users s Guide 71 2 78 Working with the Instant Pilot Method Properties The p
60. ected 04 13 07 16 00 49 pores Exit Displays the properties of an object Ly 16 00 Figure 52 Method Properties History 80 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Saving a Method Methods are stored within the Instant Pilot internal memory and or on an external USB Flash Drive The currently loaded method is also the active method in the modules Changes to the method are immediately transfered to the modules The Instant Pilot generates a list of all available methods that can be loaded The number of methods that can be stored depends on the number of timetable and injector program lines included In general more than 100 methods may be stored in the Instant Pilot With differing method contents the actual amount of methods to be stored may change significantly Use a USB Flash Drive in order to store an infinite number of methods for future use or for exchange between LC instruments see Transfer of Methods on page 83 w Method WOLF all Posttime OFF gE Quaternary Pump DE23923124 Control Stoptime OFF Posttime OFF Flow 0 000 ml min B C D OFF OFF OFF Min Pressure OFF Max Pressure 400 bar 1Load Minimum Stroke 50 pl 2 Save Compressibility 100 10E 6 bar Max Flow Gradient 100 0 ml min WA Wen STE Primary Channel Auto 4 5 Offline 6Import File Figure 53 Method File menu e Save stores the actual m
61. ectrum FLD nE M 380 385 390 395 400 405 410 nm n a Ex 350 nm Em A 417 step 1 nm Fit OFF Exit Valid from 280 to 900nm 14 33 M Cursor Cursor Peas Figure 80 Spectrum FLD Emission Spectrum FLD aa 380 Shows the peaks in the current scan a gt Ex r 7 I JR 10 43 A Cursor Cursor gt TT Peas Figure 81 Spectrum FLD Peaks Agilent Instant Pilot Users s Guide 105 2 Working DAD with the Instant Pilot D VWD FLD Spectr Warnings are shown if the method parameters are not set to produce spectra information and if the lamp in not switched on Start keys will be kept disabled until valid conditions are reached Excitation Spectrum FLD Invalid Scan Settings O Scan settings are invalid The current method settings will not generate spectra information Do you want to modify the settings such that spectra information is available we IIF 345 vo oF TTF q 08 gt Em 320 nm Ex 345 370 step 1 nm Fit forF Exit KJ A Cursor Cursor Pes TO Figure 82 Spectrum FLD 106 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Connecting External Devices There are several kinds of interface that enable the Agilent Series modules to communicate with a range of other output devices For some of them extra hardware needs to be installed Configuratio
62. emote not ready Remote start Wait x minutes Wait for ready timeout x min Wait for start timeout x min Contact x open close Repeat Start x times Repeat End Increment actual sample position x vial s ALS only Increment actual sample position w tray s x plate s y row s z column s WPS only Reset actual sample position ALS only Reset actual tray position WPS only Reset actual plate position WPS only Reset actual row position WPS only Reset actual column position WPS only Syringe to home position DLA G2258A t WPS G1367X G1377A G2258A ALS G1313A G1329A G1389A G2260A 90 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Sequence Automating Analyses If additional details are required on a specific topic function parameter not mentioned in this document please use the Instant Pilot s Online Information System i see The i info key Online Information System on page 27 You can use the Sequence screen to create completely automatic unattended analyses from sample preparation to injection The Sequence screen is accessed by pressing Sequence in the Welcome screen te Sequence UNNAMED Location Edit a line End of Sequence lal Edit Insert a line Insert Delete a line jita Delete g Copy a line Copy G Leaves the screen dle ono Exit Displays the current sequence table in a three column table ky 16 08 Tray View J Properties w
63. eout 4 Stabilization 5 End Actions Delete deletes a selected sequence line Copy copies a selected sequence line Exit or Esc exits the screen Tray View shows the current sequence s status graphically Properties The properties of a sequence can be reviewed in the Properties screen You can view changes and the reasons for them and change also the protection of he current sequence See also Sequence File Protection on page 96 Wizard The wizard allows easy definition of sample ranges and calibration processing It starts with the input of the location File Sequence parameter sets can be accessed in the internal flash disc or on a USB Flash Drive using the 2Load A H 3Save file dialog Pee 5Transfer 6Print nv moves the curser up or down in a content list OK key or 8 opens the selected parameter Agilent Instant Pilot Users s Guide 41 1 Start up Information Status Information 42 To view edit the Status information press Status from the Welcome screen System min Controller aay Quaternary Pump Stoptime Current Method Flow 0 000 B OFF 0 000 C OFF TCC cC Sa Temp Left 0 2 bar D OFF OFF 0 0 A 08 Autosampler 4 Diode Array Detector SL Speed ul min A Bw B BW Inj Vol 5 00 ul Draw 100 Sig 254 4 254 16 nm Eject 100 m Use Ref m Use Ref Ref 360 100 360 100 nm Vial yl 65 53 42 08 mAU valid from 0 00 to 99999min or OFF 11 47
64. es plus CAN cable plus CAN cable plus CAN cable The installation of older firmware might be necessary e to keep all systems on the same validated revision or e if third party control software requires a special version To upgrade downgrade the firmware 1 Download the firmware and the documentation from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 Use one of the above mentioned update tools see Table 13 The tools are also available via the above mentioned Agilent web If the Instant Pilot is in Backward compatible mode ON see With 3rd Party Control Software on page 113 it will not be seen by the Firmware Update Tool In this case reconfigure the Instant Pilot to Backward compatible mode OFF Agilent Instant Pilot Users s Guide 147 5 Maintenance and Repair Firmware Updates 2 Load the firmware into the module s as described in Updating the Firmware Using The Single Mode on page 149 or Updating the Firmware Using The Wizard on page 151 148 Agilent Instant Pilot Users s Guide Maintenance and Repair 5 Updating the Firmware Using The Single Mode CAUTION Only one firmware revision set should be stored on the USB Flash Drive If more than one firmware version for a module is available the Instant Pilot always takes the most recent firmware version In this case delete those versions not required Do not disconnect the Instant Pilo
65. ethod can have more information than the method on the Instant Pilot e g additional DAD parameters that are only accessible from the Agilent ChemStation To have a method available on both controllers proceed as follows If the method is on the Agilent ChemStation and should be saved on the Instant Pilot or USB Flash Drive load the method on Agilent ChemStation and then save the method on the Instant Pilot or USB Flash Drive with Method Save As If the method is on the Instant Pilot or USB Flash Drive and should be saved on the Agilent ChemStation first load method DEF_LC M on the Agilent ChemStation to have no additional parameter in the format and then load the required method on Instant Pilot Then save the method on the Agilent ChemStation with the same name A method that is available on the Instant Pilot as a protected method can be modified by the Agilent ChemStation and then saved on the Instant Pilot without any warning The method will be changed in the instruments but the file cannot be saved anyway 112 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 With 3 Party Control Software There may be problems when connecting the Agilent Instant Pilot G4208A to an Agilent 1100 1200 1260 1290 instrument controlled by third party software if this software generates an error when detecting an unknown module in the configuration Therefore the Agilent Instant Pilot G4208A can be made invisible
66. ethod in the Instant Pilot s internal memory e Save as allows the selective storage in the Instant Pilot s internal memory or on the external USB Flash Drive and copy delete protection functions Agilent Instant Pilot Users s Guide 81 2 82 Working with the Instant Pilot 1 Press File and select the Save as w Method File Save as Location INTERNAL ly Name Deletes a method Ll o 2 pea INTERNAL LAB_DEF 21 09 05 11 26 46 WOLF 21 09 05 11 25 12 USB Copies a method Protect unprotect the method ay ra 3 i i x gC g Exits this screen Q v 3 Qa o Saves the settings and leaves e the screen Sa lt Enter up to 8 characters fal 15 17 C A Properties Figure 54 Method Save As 2 Choose the location internal Instant Pilot or USB USB Flash Drive and a name if not already done 3 You may delete or copy methods from one location to the other 4 You may protect un protect a method see Method Properties on page 78 and Method File Protection on page 79 The stored method now contains all the current LC system and module settings If you disconnect the Instant Pilot from one LC system and connect it to another LC system the Instant Pilots current method will get an or because its settings vary from the settings of the new LC system To transfer methods from one LC system to another you can use the Insta
67. g the sequence Continue aborts or pauses depending on the selection the current sequence A shortcut is to press Stop again te Stop Analysis Esa Stop Mode Abort immediately x Exits this screen Pause sequence Cancel Pause after selected line Continue stops pauses the gt running sequence Cont Figure 75 Sequence Stop 100 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Displaying Data Graphically The Plot screen gives you many opportunities to display a wide variety of signals on a graphic display while the analysis is performed or not The plot screen can show different signals of the connected modules over time The signals are user selectable can automatically be rescaled for best on screen fitting E Status Plot DAD SL SigA TCC Temp L Selected signal active is Cy framed 27 0 26 54 26 0 25 54 25 0 24 5 24 0 Displays a plot of the selected parameters 16 49 Figure 76 Plot screen The Print button allows the print of the plot window to the USB Flash Drive Use the Setup button to select the signals of interest see also Setup of Signals on page 102 Use the Rescale button to maximize the signals of interest You can use the Direction keys to change the Y range up down or the time scale left right Use the Select button to make a signal active on the Y axis or use the number keys 1 2
68. ge 48 defined in the Configuration settings see Printing To USB Flash Drive on page 65 Printing can then be performed by opening the file with a PC Table 9 on page 48 shows the possible icons events Agilent Instant Pilot Users s Guide 47 1 48 Start up Information Logbook Information Table9 Legend of Logbook Icons Entries status change event Info event error event EMF Early Maintenance Feedback event voon sequence event a Agilent Technologies SYSTEM1 04 13 07 13 07 Instrument Logbook Class Date Time Module Message 8 04 13 07 12 51 07 DAD SL Lamp off 8 04 13 07 12 51 07 DAD SL VIS lamp off 04 13 07 12 51 18 TCC Temperature off 04 13 07 12 51 18 ITCC Column ID left data valid 8 04 13 07 12 51 18 TCC Column ID right data valid 8 04 13 07 12 51 14 Quat Pump Prerun 8 04 13 07 12 51 14 Quat Pump No analysis 8 04 13 07 12 51 18 TCC Calibration done B 04 13 07 12 51 14 Quat Pump Pump off 04 13 07 12 51 18 ITCC Valve switched to column 2 B 04 13 07 12 51 14 Quat Pump Composition ramp of 8 04 13 07 12 51 14 Quat Pump Flow ramp off 8 04 13 07 12 51 04 Autosampler No service mode 04 13 07 12 51 18 FLD Lamp off B 04 13 07 12 51 18 FLD Reference on 8 04 13 07 12 51 18 FLD Calibration done 8 04 13 07 12 51 04 Autosampler Tnitialization done B 04 13 07 12 51 18 FLD Economy mode disabled
69. gilent Series modules are ready for the next analysis this signal is on Other modules or external devices can now react e g by issuing a start request Prepare This causes the modules to get ready for the next analysis e g the detector performs a balance Start Request This signal causes the modules to get ready for the analysis e q the autosampler will begin the injection cycle As soon as all conditions to start the analysis the injection needle is placed in the seat and the valve is in the proper position are fulfilled a Start signal is generated to inform the other modules that now the analytical run starts Start In standard mode only the autosampler creates this signal This sends an order to start run time controlled activities to all the modules connected to the APG remote bus From now on moment of injection the runtime counts up If the appropriate extension board is part of your system you can use this output to inform external devices about the vial number currently processed Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 External Contacts With an optional external contacts board you have various possibilities to synchronize LC activities with external devices Communication Interfaces See also Simultaneous Execution with Software on page 111 for further details on how to operate the system using the GPIB interface CAN The module to module communication is
70. gle Agilent Module 20 to an Agilent system 19 20 Instant Pilot working with 61 interfaces 107 APG remote 107 BCD 107 GPIB 107 MIO 107 serial RS232 107 internal memory 81 internal method 75 internet 172 isocratic analysis 115 standard sample 116 K Kelvin 33 keys action 26 alphanumeric 26 direction 26 Esc 26 help 26 info 27 navigation 26 numerical 26 OK 26 overview 25 174 start stop 26 kPa 33 L LAN amp CAN connectors 20 address 37 settings 34 layout of keyboard 25 LC system shut down 92 line voltage and frequency 17 loading a method 72 logbook filter 47 information 47 M main system 146 maintenance activities 51 activity 52 feedback EMF 13 information 50 mercury fluorescent lamp 17 method asterix 73 compare 75 displayed 75 external contacts 76 file 39 file protection 13 78 79 filtering information 39 74 G1323 import 85 hash sign 73 import 85 information 38 70 injector program 86 Agilent Instant Pilot Users s Guide internal 75 loading 72 modifying 73 name description history 78 offline work 84 properties 39 78 protect 13 protection 78 save save as 81 saving 81 118 time table 39 timetable 76 trace changes 78 transfer 83 types 70 USB Flash Drive 70 working with 70 methods working with 70 MIO interface 107 modifying a method 73 module maintenance 51 specific test 55 tests 13 51 55 muliple standards 133 multiple level
71. has to be downgraded from B xx xx to A 05 15 to allow the operation with A 05 09 12 firmware installed on Agilent 1100 1200 series modules follow the procedure below 1 Copy the firmware file G4208A_A515 dlb to an USB Flash Drive the A 05 15 has only main firmware the resident of B xx xx resides in the memory 2 Insert the USB Flash Drive into the Instant Pilot Start the firmware update Upgrade to Revision B xx xx When the Instant Pilot needs to be upgraded to a B xx xx firmware to be compatible with Agilent 1100 1200 series modules working on A 06 xx follow the procedure below 1 Copy the latest firmware files 4208A_Bxxx_yyy dlb and res_4208A_Bxxx_yyy dlb to an USB Flash Drive the original resident of B xx xx in the memory will be overwritten 2 Insert the USB Flash Drive into the Instant Pilot 3 Start the firmware update Agilent Instant Pilot Users s Guide 153 5 Maintenance and Repair Firmware Updates Using the Agilent Lab Advisor 1 Start The Lab Advisor 2 Connect to the HPLC system The modules will be listed Wye Agilent Lab Advisor System Overview asks System Name System Information EMF Status Agilent LC 2873 EMF Not Ready G1321A FLD Original Type G1321B Serial DEABO03324 LAN Card 8 pl Flow Cell Firmware A 06 55 001 LAN Settings IP 10 68 10 132 0030D31D2B73 S G4208A Serial 1200 Instant Pilot DE13599999 B 02 16 0001 A Service amp Diagnos
72. htness 34 overview 25 scroll through 37 settings 32 shutoff time 34 displayed method 75 173 E early maintenance feedback EMF 13 47 EMF 47 limits 51 setup 51 end actions 92 entering settings 118 errors during firmware updates 160 Instant Pilot not recognized by Firmware Update Tool 113 162 logbook 47 Esc key 26 exchanging the CAN Cable 164 external contacts method 76 external devices 107 F Fahrenheit 33 features 12 and benefits 14 B 02 01 A 05 11 13 diagnosis with passed failed 14 early maintenance feedback EMF 13 protect method 13 setup wizards 14 file load method 72 method 39 transfer 83 file protection sequence 96 filter logbook 47 method information 39 74 firmware A 05 11 153 revisions 37 Agilent Instant Pilot Users s Guide update 146 update wizard 50 firmware update A 05 11 153 errors 160 Instant Pilot not recognized 113 162 using single mode 149 using the wizard 151 FLD spectrum 105 format amp units 32 date time pressure temperature 33 G G1323 method import 85 GLP compliance 13 graphic screenshot 114 graphical display 101 graphs 101 hash sign method 73 help 27 key 26 history of method 78 holder 163 holder versions 19 humidity 17 i info key 26 27 identify a module 51 import of methods 85 information diagnosis 55 Index logbook 47 maintenance 50 method 38 sequence 40 status 42 system 37 injector program 86 installation to a Sin
73. ialog structure easy to understand icons Automatic context sensitive help in status line Tool Tip Diagnosis with passed failed Setup wizards Dynamic adjustment to changed system configuration Method on and offline editable New sequence wizard table view priority sample insert method parameter Printing to USB Flash Drive Better readability and usability Faster and more flexible method and sequence transfer to other Agilent systems Handling for unsupported formats formatting Faster application large number of Agilent modules connectable all detector signals available in plot Fast overview of configuration and state of system Much faster confidence and usability less training required Easier parameter input through given ranges No user interpretation necessary clear result Easier system configuration and sequence setup No restart necessary when system configuration changes e g different detector Methods can be changed during runs Simpler and more flexible better overall view The files can be opened using Microsoft Internet Explorer and printed from there Agilent Instant Pilot Users s Guide Requirements for the Instant Pilot Start up Information 1 The Agilent Instant Pilot can be attached to a modular Agilent HPLC system or a single Agilent HPLC module Depending on the system the following firmware requirements must be fulfilled Table 2 Pre requisites Compa
74. ig view configuration ees ferences Figure 47 Method Compare screen Displayed Method is the actual loaded method modified e g WOLF Internal Method is the actual loaded method not modified e g WOLF If there are differences in the configuration and or timetable a message is shown in the status line and you can view the differences via Config If the configuration differs only the differences of the configuration are shown Agilent Instant Pilot Users s Guide 75 2 76 Working with the Instant Pilot Method Timetable To time program selected settings during the analysis you can create a timetable Using the Timetable screen you can create a time based program that will automatically control the modules of a system and external contacts if an external contact board is used In some cases the settings change instantaneously from the initial value to the value specified after a certain time in the timetable e g wavelength In other cases e g solvent composition these changes take place dynamically approaching the set value in a stepwise and linear manner The timetable becomes part of the current method when the method is saved The Timetable screen shows the timetable used in the currently shown method The timetable can be edited in the Timetable screen and is stored together with the method You can edit lines insert new lines copy lines and delete lines You may choose if the list should b
75. ilent Instant Pilot Users s Guide 131 4 Running Multiple Vial Analyses The autosampler now analyzes e the three calibration standards in duplicate e sample vials 10 through 14 e the three calibration standards in duplicate e sample vials 15 through 19 e the three calibration standards in duplicate e sample vials 20 through 24 and e the three calibration standards in duplicate 132 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 Re calibrating With Multiple Groups of Standards There are two different types of sample A and B that need to be analyzed The analysis for sample type A requires a 5 ul injection and a stoptime of 8 minutes The analysis of sample type B requires a 2 ul injection and a stoptime of 5 minutes For sample type A there are 3 calibration standards of different concentrations and 6 samples each sample must be analyzed once the calibration standards must be analyzed in duplicate and re analyzed after every 2 samples C1 C2 C3 S7 S8 C1 C2 C3 S9 S10 C1 C2 C3 S11 S12 C1 C2 C3 The calibration standards of type A are in positions 1 2 and 3 of the autosampler tray and the 6 sample vials are in positions 7 to 12 and the samples and the calibration standards use the same method called METH1 For sample type B there are 3 calibration standards of different concentrations and 9 samples each sample must be analyzed once the calibration standards must be
76. ilent Technologies 2006 2015 Printed in Germany 04 2015 G4208 90008 Se a Agilent Technologies
77. ink exits the online help moves the curser to previous or next link moves the curser up or down to a link shows the content as book structure How Tos Reference Concepts Error Messages Troubleshooting start page of the online information system opens the alphabetical index goes to previous screen toggle back goes to next screen toggle forward Agilent Instant Pilot Users s Guide 29 1 Start up Information Basic Operational Concept of the Instant Pilot 30 Below are is the new operation concept of the Instant Pilot described Configuration These parameters allow setup of the instrument configuration Typically these configurations are linked to properties of the instrument e g module names flow path volumes analog output configuration LAN address that are set up only at installation or after modification of the instrument setup Method amp Timetable All parameters that have an influence on the analysis result Chemists change these parameters to set up optimal conditions for a certain analysis Control Button All control menu items directly trigger a day to day action on the instrument outside an analysis The control menu can be opened in major screens via the Control button Typical functions are detector balancing or getting the instrument in a ready for analysis state Diagnosis Diagnosis tests allow the checking of proper operation They only report the state of a module with a p
78. ion called main system Resident System e the complete communication capabilities CAN LAN USB and RS 232C on the Instant Pilot only CAN e memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities CAN LAN USB and RS 232C on the Instant Pilot only CAN memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e synchronization e error handling e diagnostic functions e module specific functions 146 Agilent Instant Pilot Users s Guide Maintenance and Repair 5 The firmware of Agilent HPLC modules or the Instant Pilot can be updated using e Agilent Lab Advisor software LAN or LAN USB Infinity II Fusion e Instant Pilot and a USB Flash Drive e LAN RS 232 Update Tool 2 10 Updates via USB works only on the new 1200 Infinity II series modules with FUSION electronic platform and Lab Advisor B 02 06 and later Table 13 Firmware Update Tools Update via Instant Pilot G4208A LAN RS 232 Update Tool 2 10 Lab Advisor B 02 06 and above Module and a PC with LAN or RS 232 and a PC with LAN RS 232 USB Instant Pilot G4208A USB Flash Drive via the HPLC system plus CAN via the HPLC system plus CAN 1100 1200 1260 1290 USB Flash Drive requires LAN RS 232 requires LAN RS 232 USB HPLC Modul
79. ion 1 Start Analysis Screen With firmware revision B 02 01 and A 05 11 November 2006 the Start Analysis screen known from the G1323B Control Module has been enhanced It allows to set up a simple analysis by e pressing the START key e adding the vial range and number of injections e selecting the current or a different method internal or from USB Flash Drive e use of current sequence if active e resume paused sequence e use Blank Run e Start from selected line te Start Analysis Sample Range from Pia to P1 A 1 Inj 1 k Use Current gt Y 5 X leaves this screen Current sequence Cancel Resume paused sequence gt starts the Analysis when all Blank Run Cont modules are ready Start from selected line iPress Enter to edit settings Figure 37 Analysis Start Screen Agilent Instant Pilot Users s Guide 57 1 Start up Information Switching from G1323A B Control Module to Instant Pilot The Instant Pilot is a further development of the G1323A B Control Module which has been reworked and structured in a new modern way more like an Agilent ChemStation Therefore some areas of the G1323A B Control Module appear in different areas Table 10 shows the main changes Table 10 G1323A B Control Module vs Instant Pilot Functions G1323A B Control Module G4208A Instant Pilot Comment Analysis screen Analysis screen Settings Method Analysis screen Time T
80. itional details are required on a specific topic function parameter not mentioned in this document please use the Instant Pilot s Online Information System i see The i info key Online Information System on page 27 A method contains a complete set of injection separation and detection parameters including the timetable and injector program The sample position information is not part of the method There are two types of methods e The Instant Pilot method The method is stored in the internal memory of the Instant Pilot The actual method s parameters are stored in the individual LC modules A method that is stored in the individual LC modules can be loaded modified saved and run from the Instant Pilot e The USB Flash Drive method The method parameters are stored on a USB Flash Drive A method that is stored on the USB Flash Drive can be loaded to the LC modules or transferred to another LC system Methods cannot be run directly from the USB Flash Drive The method must first be loaded from the USB Flash Drive before it can be run When the USB Flash Drive method is loaded it becomes the current module method Unless stated otherwise the following sections refer to module methods To view edit the method information press Method from the Welcome screen 70 Agilent Instant Pilot Users s Guide Working with the Instant Pilot Method UNNAMED Pump i ci 20 00 min
81. its Primary Channel Auto xj Done the screen Specifies a time limit for your analysis KS 15 19 Figure 56 Method Save As All buttons have the same function as in the online method dialog see Modifying a Method on page 73 Only the Control button is removed and the Exit button is replaced with a Done Cancel Agilent Instant Pilot Users s Guide Import of Methods Working with the Instant Pilot 2 This functions allows the import of G1323 Control Module methods stored on the instrument or on the USB Flash Drive Export is not possible L Internal G4208A 21 09 05 11 25 12 w Method Import Imports a selected method Deletes a method G1323 Methods from USB G1323 Methods from Instrument Exits the screen Exit A 15 19 Coo A Properties Pd Figure 57 Method Import G1323 methods from USB G1323 methods from instrument Agilent Instant Pilot Users s Guide generated with G1323 Control Module then transferred via G1323 PC card PC USB Flash Drive to the Instant Pilot generated with G1323 Control Module transferred via HPLC module to the Instant Pilot 85 2 86 Working with the Instant Pilot with Methods Injector Program With firmware revisions B 02 01 and A 05 11 November 2006 the Injector Program has been implemented The injector program is part of the method The injector program screen can be accessed by pressing edit on the Injec
82. l events are listed even duplicates up to 1000 entries This logbook can be saved to the USB Flash Drive Screenshots also can be saved to the USB Flash Drive see Saving a Screenshot to USB Flash Drive on page 114 Troubleshooting the Instant Pilot If your Instant Pilot does not work correctly disconnect the module CAN connector from the rear of the Agilent Series module it is attached to and reconnect it If the problem still remains then e power down all connected devices and computers and wait 1 minute and then restart or e try to use just one Agilent Series module If the problem still remains call Agilent Technologies USB Flash Drive not recognized Since USB Flash Drives may vary from vendor to vendor or from type to type incompatibilities can occur In general USB Flash Drives from Sandisk and Kingston should work The USB Flash Drive must be FAT 16 formatted and without encryption See Handling of Unsupported USB Flash Drive Formats on page 63 and USB Flash Drive Kit on page 163 Agilent Instant Pilot Users s Guide 161 5 Maintenance and Repair Instant Pilot not recognized by Firmware Update Tool If the Instant Pilot is in Backward compatible mode ON see With 3rd Party Control Software on page 113 it will not be seen by the Firmware Update Tool In this case reconfigure the Instant Pilot to Backward compatible mode OFF Contact Agilent If you experience other problems
83. lculated by 100 B C D If no values for B C and D are entered A is always 100 Saving Settings in a Method 118 Select File in the Method Information screen 2 Select Save As 3 Enter the method name as ISO using the selection keys also see Saving a Method on page 81 4 Press Save to save the method Press Exit to leave the Method screen Agilent Instant Pilot Users s Guide Running an Isocratic Analysis 3 Creating a Sequence Select Sequence in the Welcome screen Press Insert and select Method Select Method named ISO and press OK Aa QO N Press Insert and select Sample If your sample is not in vial 1 you have to modify the vial number also see Sequence Automating Analyses on page 91 5 Select File and select Save As Enter the sequence name as ISO using the numeric keys see also Saving a Sequence on page 95 7 Press Save to save the sequence 8 Press Exit to leave the Sequence screen Selecting a Signal Setup the signal parameters in the Method Information screen Select Status in the Welcome screen Press Plot Press Setup oa A O N Choose a Detector Signal from the Available Signals list You can choose several signals at a time The plot function will display all signals that are shown in the Selected Signals list box Use the selection keys to navigate within the list box and select the signal s 6 Press Done
84. le editable and Analysis for the large analysis tiles In the setup dialog it is checked on Done that an analysis tile is alone in a large quadrant Move to a field and press Select Agilent Instant Pilot Users s Guide Start up Information 1 atio E Status Setup Select Status of System Stoptime Editable Posttime Posttime Editable Controller DE12345678 Seq Sample Seq Status Current Method _ xX leaves this screen without Binary Pump DE00000000 Analysis Cancel changes Not Ready State d select a signal parameter Flow Editable x Select Allows you to set up the status screen ky 17 47 Figure 22 Status Screen Select From this list select a signal parameter and press Select The selection will be taken for the selected window Figure 23 shows the relation of the windows in the Setup screen versus displayed windows Status Setup DEFAULT System min Controller aay Quaternary Pump Cantraller Quat Pump Ei Stoptime Current Method A Current Method Analysis Select Flow 0 000 mM B OFF ill m 0 000 C OFF U P Temi pa D oF L clear Temp Left 0 2 bar Editable OFF 0 0 A 0 8 U rE i DAD SL Cut Autosampler X Diode Array Detector SL Anahyaler er Analysis gt Speed l min SA A BW B BW x Inj volf 5 00 pl Draw 100 Sig 254 4 254 16 nm i 3 Cancel Eject 100 M Use
85. lent Instant Pilot Users s Guide 49 1 Start up Information Maintenance Information 50 The Instant Pilot provides basic maintenance and diagnostic functions only The Agilent LabAdvisor software provides the full maintenance and diagnostic capabilities To view change the Maintenance information press More from the Welcome screen and select Maintenance from the menu g Maintenance System j Module Product Serial Firmware Controller G4208A DE55055002 B 02 01 0001 S Quat Pump G1311A DE23923124 A 06 01 012 Autosampler G1329A DE91603245 A 06 01 012 rN update a set of modules Col Comp G1316A DE14923865 A 06 01 012 Nicaea 3 update a single module Single DAD G1315B DE03010634 A 06 01 012 to change the product number or serial number after main board exchange G leaves this screen Exit 13 48 System I Controller Quat Pump TAutosampler po bein or module specific information 2 Sik la Displays information on the available modules r 7 Figure 29 Maintenance Screen The Maintenance System screen shows a list of all modules in the system with their names product and serial numbers and the firmware revision You can update the firmware using Update Wizard which allows updating all modules of the system at once or using Single to update a selected module The firmware must be on an inserted and activated USB Flash Drive in its root dire
86. longer accessible Details can be found in the manual provided with the HPLC module i Maintenance PN SN Change L When the main board has to be replaced the new board does not have a serial number For some modules e g pumps or autosamplers also the product number has to be changed multiple usage boards Use the information from the serial number plate of your module The changes become active after the reboot of the module Product No 6131 5B Serial No DE03010634 Warning If you enter the wrong type your module might not A be accessible anymore Done Enter up to 11 characters ky 13 51 Figure 34 Maintenance Screen Product Number and Serial Number Change Agilent Instant Pilot Users s Guide Start up Information 1 Diagnosis Information The Instant Pilot provides basic maintenance and diagnostic functions only The Agilent LabAdvisor software provides the full maintenance and diagnostic capabilities To perform a module specific test press More from the Welcome screen and select Diagnosis from the menu Diagnosis 3 Exec Quaternary Pump DE23923124 Autosampler DE91603245 Injector Steps Column Compartment DE14923865 Diode Array Detector DE03010634 Lamp intensity test Holmium spectrum test Dark current test Cell test No Passed Fail result EA RY 83 02 Figure 35 Diagnosis Screen The Diagnosis screen shows a list
87. lot i Agilent Technologies 1 1 Start up Information Instant Pilot Features 12 wre BLS J 0063 am giroa DERA oa Com ainsa pE618001 9 j paet Figure 2 The Agilent Instant Pilot The Agilent Instant Pilot IP provides complete local control and monitoring of a single module or an entire Agilent 1100 1200 1260 1290 Series HPLC system You have easy access to every supported function you can easily control all parameters and settings and you can configure various communication channels with other devices in order to comfortably analyze the generated data e Color TFT display size 13 1 x 9 9 cm 5 0 x 3 8 inch 640 x 480 dots e Processor 400 MHz 64 MB RAM 32bit e Install any desired configuration of Agilent 1100 1200 1260 1290 Series HPLC modules The Instant Pilot software will reflect which modules are present in the LC system and adjust the screens accordingly e Enter parameter settings for every module perform on off functions as well as calibration and configuration settings in a self explanatory and intuitive way e Define automated analyses including methods timetables method sequences and automated calibration settings using the Instant Pilot Agilent Instant Pilot Users s Guide Start up Information 1 e Use the configurable status screen to monitor various activities on a single screen e Easy configuration of the system and or modules e Protect your method from any i
88. lts to avoid crystallization or to program a soft shut down method using Insert Method or turn OFF the LC system using End Actions You can set up automatic re calibration using the Calibration Settings screen The Calibration Settings screen is accessed by selecting Calibration in the Sequence Wizard screen You can re calibrate using one or more standards and have the flexibility to choose various calibration intervals and patterns You can define the frequency to re calibrate and the order of calibration vial analysis using the Alternate and Multi settings Alternate analyzes the calibration vials alternately Multi analyzes the calibration vial or vials in complete groups according to the calibration interval Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Sequence Automating Analyses Using the Sequence Wizard You may use the Wizard to set up a sequence Wizard Samples From fi To 5 Num Inj X Cancel Done valid from 1 to 50 amp 14 56 Samples Calibration Preview Figure 65 Sequence Wizard Adding Samples Information Wizard Calibration m Use Calibration Calibration SSS From fio TT See Figure 67 and Figure 67 on age 94 for effect of selection To f2 oe p g on u u Multi and Alternate Num lnj C Before m Every 2 Samples Cancel M After vi Done Allows you to set up calibration samples in the sequence table ky 15 57 Samples Calibra
89. mple range possibility is for running sequences without parameter changes for the specified method s i amp Start Analysis Sample Range from to P1 A 1 Inj 1 f lt Use Current gt iY C vent Sequence x Exits this screen Current sequence Cancel Resume paused sequence gt Continues the activity Blank Run Cont Start from selected line Press Enter to edit settings Figure 72 Sequence Start You may press Start again to bypass the above screen If any actions are still required to get the system into a ready state the Get Ready screen will show up Figure 73 on page 99 otherwise e the system status changes to green e the sequence starts immediately and e the last screen shows up Press Status to display the actual system status Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Sequence Automating Analyses If any activities before the system ready gray status are still required press Continue and all actions e g required lamps are turned on are performed automatically Start Analysis Get Ready Yellow status indicates a not ready condition Interactive Action by user required Automatic Press continue to perfom actions Quat Pump Switch pump on Col Comp Switch temperature control on DA Det Switch UY lamp on DA Det Switch VIS lamp on Temporary Transient not ready condition x Exits this screen Cancel
90. n analysis 115 Agilent Instant Pilot Users s Guide S safety information standards 17 sample range 98 save method 81 saving method 81 118 screens plot 101 sequence 91 screenshot saving to USB Flash Drive 114 scroll through display 37 selecting a signal 119 sequence 91 127 129 calibrate and recalibrate 92 calibration 92 127 129 create 119 end actions 92 file protection 96 information 40 modes 138 preview 94 properties 38 40 protect un protect 96 protection 96 recalibration 92 screen 91 start and stop 98 tray view 40 97 what is a sequence 91 wizard 40 serial interface 107 serial number change 54 serial numbers 37 service firmware update 146 Index the Instant Pilot 145 settings brightness 34 date amp time 32 date format 33 display 34 EMF 47 entering 118 LAN 34 pressure format 33 temperature format 33 time format 33 units amp formats 33 setup signals 102 status information screen 44 wizard 14 31 49 shutdown signal 107 shutoff time display 34 signal selecting 119 setup 102 signal unit axis 103 single level calibration 127 solvent composition 117 spectrum DAD MWD VWD FLD 104 FLD 105 standard isocratic sample 116 standard mode 138 start stop keys 26 start of analysis 57 start request signal 108 138 139 external 141 start signal 108 138 starting and stopping a sequence 98 status colors 31 42 176 information 42 information screen setup 44
91. n and OpenLab can be connected to an Agilent Series system at the same time Parameter entry is possible from both user interfaces Parameters are updated on the other user interface within a few moments An Agilent ChemStation sequence can be stopped and aborted from the Instant Pilot and vice versa The Agilent ChemStation can generate data files from a Instant Pilot method or sequence In this case the pre fix and file name counter in the Single Sample Info section of the Agilent ChemStation must be enabled protocol mode only If the Instant Pilot starts an analysis the Agilent ChemStation is the slave monitor system The Agilent or 3rd party user interface may block certain functionality e g editing load start or others In such a case the screen shows a blinking lock symbol in the top left Restrictions If a parameter window is open for parameter entry on the Agilent ChemStation this specific entry field is disabled on the Instant Pilot If an analysis is running with the Instant Pilot the Agilent ChemStation must not be turned on If the Agilent ChemStation starts an analysis the Instant Pilot is the slave monitor system Parameter changes to a method will be identified on the other user interface as modification Agilent Instant Pilot Users s Guide 111 2 CAUTION Working with the Instant Pilot e The Instant Pilot and the Agilent ChemStation have a different method handling Agilent ChemStation m
92. n of selected interface parameters is possible and is handled individually for each module since some interfaces are only available with certain modules depending on installation For further information on interfaces see the corresponding sections in the modules user or reference manuals APG Remote Enhanced Remote Infinity Il The system can communicate with external devices via APG remote connector included in all modules in order to synchronize the analyses This is necessary when an external device needs some time to get ready for a new analysis and thus the transmission of a start request is required see also Synchronizing Analyses with External Devices on page 137 for details on sequence modes Detailed descriptions of the APG Remote connector are available in the HPLC modules reference manuals Among the available signals are Power On This signal is active as soon as all modules connected to the system are switched ON Shut Down When the system has a serious problem e g a leak occurs this alerts all modules to stop relevant operation in order to reduce safety risks Agilent Instant Pilot Users s Guide 107 2 108 Working with the Instant Pilot BCD Stop This signal asks all modules to reach the ready state as soon as possible It works only during the analytical run controlled by the stoptime setting and causes the system to begin counting down the postrun time Ready When all A
93. n on the method screen Compare a tool that allows you to compare two methods The differences are shown in a list by displaying the values from both methods Using the same color for method names and method parameters does the mapping between them You can copy parameters between the two selected methods using the Copy buttons Time Table used in the currently shown method The timetable can be edited in the timetable screen and is stored together with the method You can edit lines copy paste lines delete lines and insert new lines You can choose if the list should be ordered by module default or time Properties The properties of a method can be reviewed in the Properties screen You can view changes and the reasons for them and change also the protection of the current method File Method parameter sets can be accessed in the fioa internal flash disc or on a USB Flash Drive usingthe 2Save file dialog Method definitions from a G1323 Control Pare ie i P ranster Module can be imported They will be transferredto 5 offline internal flash disc instead 6lmport Z Print Another feature is the ability to edit methods offline It is possible to edit methods that were not actually loaded onto the modules You can select the method you want to edit in the Files dialog and load it into editor by pressing Load You can move files between storage locations by using Copy and Paste buttons Print saves all displayed information to the USB Flash D
94. nadvertent keyboard changes by setting method file protection e Use USB Flash Drive to store and transfer methods and sequences between Agilent systems e Monitor all operations and error events using the self updating logbooks e Use the context sensitive online information system to get further information on all topics e To help comply with Good Laboratory Practice GLP regulations select a variety of module tests that will check the performance of the LC system e Use the early maintenance feedback EMF limits for scheduling maintenance work e Display data graphically using the Plot screen where as many as four different signals can be monitored at the same time e Printing to an USB Flash Drive e Version A 05 15 is compatible with 1100 1200 modules that run on firmware A 05 11 13 and A 05 09 10 introduced November 2006 Firmware revision A 05 13 14 does not run on Instant Pilot modules with serial numbers starting with MY due to new flash ROM type that does not allow downgrades to B 02 07 and below This was fixed with firmware A 05 15 and B 02 15 Agilent Instant Pilot Users s Guide 13 1 Start up Information Features and Benefits 14 Table 1 Features and Benefits Feature Benefit Large size color TFT display with background light high resolution and contrast USB port USB Flash Drive State of the art electronic System visualization with status display in start screen Flat d
95. nd the Agilent Instant Pilot Working with the Instant Pilot This chapter describes the operation of the Instant Pilot Using the Agilent Series LC System With Control Module This part describes how to run isocratic and multiple vial analyses using a single method or more than one method Running an Isocratic Analysis This chapter describes how to analyze the Agilent Technologies isocratic standard sample using a single injection analysis Running Multiple Vial Analyses This chapter describes how to setup multiple vial analyses using the same method and different methods Support of the Instant Pilot Maintenance and Repair This chapter describes how to perform firmware updates troubleshooting and replacements Appendix This chapter provides safety and other general information Agilent Instant Pilot Users s Guide Contents 1 Start up Information 11 Instant Pilot Features 12 Features and Benefits 14 Requirements for the Instant Pilot 15 Physical Specifications 17 Cleaning 18 Holder Versions for the Instant Pilot 19 Adding the Instant Pilot to an Agilent HPLC Module 20 1100 1200 1200 Infinity Series 21 1200 Infinity Il Series 22 Removing the Instant Pilot 24 Instant Pilot Display and Keyboard Layout 25 The i info key Online Information System 27 Basic Operational Concept of the Instant Pilot 30 Getting Started 31 System Information 37 Method Information 38 Sequence Information 40 Status Inf
96. nj 3 16 Select Done to accept entries 17 Press Start and confirm the start of the sequence 126 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 Single Level Calibration Sequences The following procedure describes how to set up a calibration sequence for an analysis which uses single level calibration There is one calibration standard C and 9 samples S The analysis requires that each sample is analyzed in duplicate the calibration standard is analyzed once before the samples and re analyzed once after every 2 samples C S S C S S C S S C S S C S C the calibration standard is located in position 90 and the 9 sample vials are in positions 1 to 9 of the autosampler tray and the method called METH1 is used for the samples and standards The example assumes that the sequence table is empty If the sequence table is not empty use the Delete button to delete all lines or use File New 1 2 3 4 Select the Sequence screen Move to line 1 and press Insert Select Method and then move to METHI and press Load Move to the End of sequence list select the Wizard and enter Vial Range 1to9 lnj 2 Select Calibration to display the Calibration Settings screen Agilent Instant Pilot Users s Guide 127 4 Running Multiple Vial Analyses Single Level Calibration Sequences 6 Change the settings according to Figure 85 Wizard Calibration V Use Calibration Calibration
97. nt Pilot or a USB Flash Drive Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Transfer of Methods The File Transfer dialog allows you to transfer files between internal file storage and the connected USB Flash Drive w Method File Transfer Location INTERNAL ly Name LAB DEF 2 Deletes a method Delete Fn Copies a method i 11 26 46 aa P WOLF 21 09 05 11 25 12 Copy Protect unprotect the method Transfers the method E w W D z oO a 2 Trans Exits this screen g Exit Rl 15 18 o A Properties TO Figure 55 Method Transfer 1 Select a method 2 Press Transfer The method is transferred Agilent Instant Pilot Users s Guide 83 2 84 Working with the Instant Pilot Offline Work on Methods The Import dialog gives you the ability to edit methods offline It is possible to edit methods that were not actually loaded onto the modules The offline method dialog starts with a copy of the actual method The offline mode is emphasized by the different dialog color w Method Offline UNNAMED all Edits a method Posttime Quaternary Pump DE23923124 Stoptime OFF Posttime OFF Flow 0 000 ml min B C D OFF OFF OFF Min Pressure OFF Exi xits the screen Max Pressure 400 bar x t Minimum Stroke 50 yl Caine Compressibility 100 10E 6 bar Max Flow Gradient 1100 0 ml min iy Saves the information and ex
98. nto the module WARNING Do not let liquid drip into the module It could cause shock hazard and it could damage the module 18 Agilent Instant Pilot Users s Guide Start up Information 1 Holder Versions for the Instant Pilot Holder Versions for the Instant Pilot Mid of 2007 the holder of the Instant Pilot was introduced with a revised design Features of new holder are e easy to use e safe placement of the Instant Pilot e stable mechanism e easy upgrade of all Instant Pilot s with old holder version possible see Parts Identification on page 163 Figure 3 shows all holder versions In the actual versions the Instant Pilot is hanging in the new holder and standing in the obsolete holder original 1100 design obsolete 1100 1200 1200 Infinity design 1200 Infinity II design Figure 3 Holder Versions Agilent Instant Pilot Users s Guide 19 1 Start up Information Adding the Instant Pilot to an Agilent HPLC Module CAUTION The CAN connectors are similar to LAN adapter connectors Do not insert LAN connectors into the CAN or vice versa since the CAN uses 24 V and might damage the LAN card 20 Preferred orientation of the Instant Pilot is hanging at the HPLC modules When inserting the adapter to an Agilent Wellplate Autosampler or Fraction Collector the Instant Pilot must be removed first Otherwise the Autosampler door cannot be opened To attach the Instant Pilot to an
99. number of sequences for future use or exchange between LC instruments see Transfer of Methods on page 83 t Sequence 1 Location V1 V5 2 End of Range End of Sequence 2Load 3 Save 4 5 Transfer 6Print Tray View Properties Wizard _ Figure 69 Sequence File menu e Save stores the current sequence into the file it was loaded from e Saveas allows the selective storage in the Instant Pilot s internal memory or on the external USB Flash Drive and copy delete protection functions 1 Press File and select the Save as Agilent Instant Pilot Users s Guide 95 2 96 Working with the Instant Pilot w Sequence File Save as Location INTERNAL x Name EN x Deletes a sequence INTERNAL BEN 13 09 05 15 14 13 USB Copies a sequence E Protect unprotect the Protect sequence x Exits this screen bd Saves the settings leaves the Save screen Enter up to 8 characters KS 15 18 C A Properties TO Figure 70 Sequence Save As 2 Choose the location internal Instant Pilot or USB USB Flash Drive and a name if not already done 3 You may delete or copy sequences from one location to the other Sequence File Protection You may protect un protect a sequence see Method Properties on page 78 and Method File Protection on page 79 Differences are e Edit Insert Delete Cop
100. o Balance is set to pre run when receiving a start command from the Instant Pilot This will only happen in the Standard and Wait for single multiple start request modes In the Send single multiple start request modes a balance before the run will NOT be performed If regular balancing is required set the Auto Balance check box to Postrun Agilent Instant Pilot Users s Guide 143 4 Running Multiple Vial Analyses Synchronizing Analyses with External Devices 144 Agilent Instant Pilot Users s Guide G4208A Instant Pilot User s Guide 5 Maintenance and Repair Firmware Updates 146 Updating the Firmware Using The Single Mode 149 Updating the Firmware Using The Wizard 151 Update Information for A 05 13 Firmware 153 Using the Agilent Lab Advisor 154 Using the LAN RS 232 Firmware Update Tool 156 Errors During Firmware Updates 160 Troubleshooting 161 Repairing the Instant Pilot 163 Parts Identification 163 Exchanging the CAN Cable 164 This chapter describes how to perform firmware updates troubleshooting and replacements fs Agilent Technologies 145 5 Maintenance and Repair Firmware Updates The Agilent HPLC modules and the Instant Pilot have firmware installed that will be updated from time to time to add new features and or remove malfunctions The firmware of the instrument consists of two independent sections e a non instrument specific section called resident system e an instrument specific sect
101. of functions were added to ensure protected file security online and offline If a file is protected the user can not edit the currently loaded method content or its filter settings Edit Filter and Save buttons are disabled Enter edit mode by pressing Enter button is disabled Save As under a different name is allowed and will be stored under the new name unprotected Using the same name results in File Save Failed Permission denied error Renaming a protected file is not allowed Transfer of protected file is allowed if not a protected file with the same name already exists in the targeted destination Then the user has to unprotect the protected file on target first Import fails if a protected method with the same name on the Instant Pilot already exists In the files dialogs a protected file can be copied but not renamed or deleted Copy a protected file makes an unprotected copy under a different name on the same medium To unprotect a file the user has to enter the correct password A password to protect a file can have up to 12 digits If left empty no empty password will be added to the file protection Agilent Instant Pilot Users s Guide 79 2 Working with the Instant Pilot Working with Methods Enter Password rrr Reenter Password Figure 51 Method Protection Method Properties Name M1 a Description i ST Created from Actual 6 Changed content Protect Prot
102. or the LC system to equilibrate This way of combining methods is called Sequence e The first 20 vials are analyzed using METH1 with one injection per vial ethe next 20 vials are analyzed using METH2 with two injections per vial ethe last 10 vials are analyzed using METH3 with three injections per vial The vials are located in positions 1 to 50 of the autosampler tray The example assumes that the sequence table is empty If the sequence table is not empty use the Delete button to delete all lines or use File New 1 Select the Sequence screen 2 Move to line 1 and press Insert 3 Select Method and then move to METHI and press Load 4 Move to the End of sequence list select the Wizard button and enter Vial Range 1 to 20 lnj 1 5 Select Done to accept entries 6 Move to the End of sequence list and select Insert 7 Select Method and then move to METH2 and press Load Agilent Instant Pilot Users s Guide 125 4 Running Multiple Vial Analyses 8 Move to the End of sequence list select the Wizard and enter Vial Range 21 to 40 lnj 2 9 Select Done to accept the entries 10 Move to the End of sequence list and select Insert 11 Select Method and then move to METH3 and press Load 12 Move to the End of sequence list and select Insert 13 Select Wait time and enter Wait 30 minutes 14 Select Done to accept entries 15 Move to the End of Sequence list select the Wizard and enter Vial Range 41 to 50 l
103. ormation 42 Setup of a Status Information Screen 44 Logbook Information 47 Configuration 49 Maintenance Information 50 Agilent Instant Pilot Users s Guide Contents Early Maintenance Feedback EMF 53 Product Number and Serial Number Change 54 Diagnosis Information 55 Turning Modules ON OFF Standby 56 Start Analysis Screen 57 Switching from G1323A B Control Module to Instant Pilot 58 Information on Firmware 60 Working with the Instant Pilot 61 Using a USB Flash Drive 62 Handling of Unsupported USB Flash Drive Formats 63 Printing To USB Flash Drive 65 Working with Methods 70 Loading a Method 72 Modifying a Method 73 Filtering Method Information 74 Compare Methods 75 Method Timetable 76 Method Properties 78 Method File Protection 79 Saving a Method 81 Transfer of Methods 83 Offline Work on Methods 84 Import of Methods 85 Injector Program 86 Sequence Automating Analyses 91 Using the Sequence Wizard 93 Saving a Sequence 95 Sequence File Protection 96 Tray View 97 Starting and Stopping a Sequence 98 Agilent Instant Pilot Users s Guide Contents Displaying Data Graphically 101 Setup of Signals 102 Rescaling the Plot Screen 103 DAD MWD VWD FLD Spectrum 104 FLD Spectrum 105 Connecting External Devices 107 APG Remote Enhanced Remote Infinity Il 107 BCD 108 External Contacts 109 Communication Interfaces 109 Simultaneous Execution with Software 111 With Agilent ChemStation 111 With 3rd Pa
104. ot compatible not compatible not compatible not compatible not compatible not compatible not compatible not compatible A 05 09 10 A 05 11 12 13 A 05 09 10 A 05 11 12 13 Instant Pilot Firmware Revision B 01 02 03 04 B 01 02 or above B 01 02 or above not compatible not compatible not compatible not compatible not compatible not compatible A 06 02 or above A 06 02 or above Instant Pilot Firmware Revision B 02 01 and above B 01 02 or above B 01 02 or above B 02 01 or above B 02 01 or above B 02 07 or above B 02 07 or above B 02 07 or above not compatible A 06 02 or above A 06 02 or above Since USB Flash Drives may vary from vendor to vendor or from type to type incompatibilities can occur In general USB Flash Drives from Sandisk and Kingston should work The USB Flash Drive must be FAT 16 formatted and without encryption See USB Flash Drive Kit on page 163 CAUTION The operator of this instrument is advised that if the equipment is used in a manner not specified in this manual the protection provided by the equipment may be impaired The Instant Pilot may be used only with the Agilent instruments The Instant Pilot is designed to operate in a typical electromagnetic environment EN61326 1 where RF transmitters such as mobile phones should not be used in close proximity 16 Agilent Instant Pilot Users s Guide Physical Specifications Table3 Physical Specific
105. release button pin must fit into the recess on one end of the connecting rod Agilent Instant Pilot Users s Guide 165 5 Maintenance and Repair Repairing the Instant Pilot 10 Carefully replace the rear panel and fit the release button pin into the recess on one end of the connecting rod of the rear panel see Figure 110 release button rear panel with recess in rod Figure 110 Pin of release button fits into the recess of the rod OLD rear panel design 11 Fix the rear panel screws 166 Agilent Instant Pilot Users s Guide G4208A Instant Pilot User s Guide 6 Appendix Safety Information 168 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 170 Radio Interference 171 Agilent Technologies on Internet 172 This chapter provides safety and other general information z Agilent Technologies 167 6 Appendix Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements Safety Symbols Table 15 shows safety symbols used on the instrument and in the manuals Table 15 Safety Symbols Symbol Description The app
106. rintable Information Dialog Name Button File Name in Comment PRINTOUT System Details Print SYSINFO MHT via Details button see Figure 41 on page 66 Method File Print METHOD MHT Contains Method Timetable Inj Programm Sequence File Print SEQUENCE MHT Logbook Print LOGBOOK MHT Plot Print PLOT MHT via Status button single or multiple pressure composition temperature detector signals SCANs via Control button DAD MWD Scan Print DETSCAN MHT sample scan VWD Scan Print DETSCAN MHT blank reference and sample scan FLD Scan Print DETSCAN MHT Excitation or Emission scan Calibrations via More button and Maintenance DAD Calibration Print DADCALIB MHT MWD Calibration Print MWDCALIB MHT VWD Calibration Print VWDCALIB MHT FLD Calibration Print FLDCALIB MHT not implemented yet Diagnostic via More button and Diagnosis shows diagram actions results and sign off see Figure 42 on page 69 DAD MWD Intensity Print DIAGRES MHT DAD MWD Holmium Print DIAGRES MHT DAD MWD Dark Current Print DIAGRES MHT DAD MWD Cell Test Print DIAGRES MHT VWD Intensity Print DIAGRES MHT with Raw Sample Reference Signal Counts VWD Holmium Print DIAGRES MHT Agilent Instant Pilot Users s Guide 67 2 Working with the Instant Pilot Table 11 Overview of Printable Information Dialog Name Button File Name in Comment PRINTOUT FLD Intensity Print DIAGRES MHT Pressure Tests ISO Pump Bin Pump Micro Print DIAGRES MHT Pump Normal Quad Press High Flow Pump
107. rive into folder PRINTOUT as METHOD MHT nv moves the curser up or down in a content list OK key or 8 starts the editing of the selected parameter Agilent Instant Pilot Users s Guide 39 1 Start up Information Sequence Information 40 To view edit the sequence information press Sequence from the Welcome screen Peal Sequence UNNAMED L Location End of Sequence oho 5 a D Oo o a T Cc e py ae a al Displays the current sequence table in a three column table 16 08 Tray View Properties Wizard Fie Figure 19 Sequence Screen normal view A sequence consists of a list of items that should be processed from top to bottom The items are inserted in the list using the Insert button or in case of samples and calibration samples by using the Wizard The Sequence can be edited using the Edit Delete or Copy buttons The Tray View button shows the current sequence s status graphically The Properties button opens the history protection of the current sequence see Sequence File Protection on page 96 Agilent Instant Pilot Users s Guide Start up Information 1 Table 7 Sequence Functions of Keys Button Description Edit Insert inserts a new line with an actions from a menu for ay 1 Sample details refer to Instant Pilot s Info System gt Method 3 Parameter 4 Wait time From copy 2 Wait until 3 Not ready tim
108. roperties of a method can be reviewed in the Properties screen The user can view change history e The method s name This string is used as unique identification of the method and is also used as the filename e The description allows you to describe the method more precisely e The history shows all changes done e The method can be protected unprotected with a password Method Properties Name 456 Edit the method name Description Jno comment when unprotected Enter a method description when unprotected Created from Actual 02 28 07 Protect unprotect the method Changed content 02 28 07 16 05 59 Protected 02 28 07 16 08 19 Protect requires correct password Unprotected 02 28 07 16 08 51 Protected 02 28 07 16 10 28 G Exits this screen Y Exit Displays the properties of an object Ey 16 28 Figure 50 Method Properties screen The method can be protected against inadvertent changes Any change to the method is not accepted until the method is unprotected or by saving it again without protection Any unauthorized method or instrument changes can be traced by the system logbook The Protection button is available in all File operations For more information see Method File Protection on page 79 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Method File Protection With firmware revsion B 02 05 May 2007 several additional checks and disabling
109. rty Control Software 113 Special Functions 114 Saving a Screenshot to USB Flash Drive 114 3 Running an Isocratic Analysis 115 What You Will Need 116 Preparing the LC System 117 Entering Settings 118 Saving Settings ina Method 118 Creating a Sequence 119 Selecting a Signal 119 Observing the Chromatogram 120 4 Running Multiple Vial Analyses 123 Analyzing Multiple Vials Using the Same Method 124 Analyzing Multiple Vials Using Different Methods 125 Single Level Calibration Sequences 127 Agilent Instant Pilot Users s Guide 7 Contents Multiple Level Calibration Sequences 129 Re calibrating With the Same Group of Standards 129 Re calibrating With Multiple Groups of Standards 133 Synchronizing Analyses with External Devices 137 Standard Mode 138 Send Single Start Request 139 Send Multiple Start Request external controlled injector 140 Wait for Single External Start Request 141 Wait for Multiple Start Request Instant Pilot controls injector 142 Maintenance and Repair 145 Firmware Updates 146 Updating the Firmware Using The Single Mode 149 Updating the Firmware Using The Wizard 151 Update Information for A 05 13 Firmware 153 Using the Instant Pilot 153 Using the Agilent Lab Advisor 154 Using the LAN RS 232 Firmware Update Tool 156 Errors During Firmware Updates 160 Troubleshooting 161 Troubleshooting the Instant Pilot 161 USB Flash Drive not recognized 161 Instant Pilot not recognized by Firmware Update Tool 162 Contact
110. s present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting Agilent Instant Pilot Users s Guide 169 6 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC 170 The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent offi
111. ses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or asa commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclo sure of Software is subject to Agilent Tech nologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a hazard
112. ssing application 114 Agilent Instant Pilot Users s Guide s G4208A Instant Pilot User s Guide 7 ee 3 Running an Isocratic Analysis e 7 e j What You Will Need 116 Preparing the LC System 117 Entering Settings 118 Saving Settings in a Method 118 Selecting a Signal 119 Observing the Chromatogram 120 This chapter describes how to analyze the Agilent Technologies isocratic standard sample using a single injection analysis i fe Agilent Technologies 115 3 Running an Isocratic Analysis What You Will Need 116 Instruments Column Solvents Sample Agilent Series isocratic binary or quaternary pump an autosampler and a UV detector A 125 mm x 4 0 mm Hypersil ODS 5 um Agilent Technologies part number 7982618 564 For the isocratic pump a solvent mixture of LC grade bidistilled water 35 and acetonitrile 65 The Agilent Technologies isocratic standard sample Agilent Technologies part number 01080 68704 This contains 0 15 wt dimethylphthalate 0 15 wt diethylphthalate 0 01 wt biphenyl and 0 03 wt o terphenyl dissolved in methanol The descriptions are based on Agilent 1100 1200 systems The Agilent 1290 Infinity System may have other requirements on the setup and material Agilent Instant Pilot Users s Guide Running an Isocratic Analysis 3 Preparing the LC System 1 For the isocratic pump fill the solvent bottle with the mixture of LC gr
113. ssure vy Max Pressure Minimum Stroke lt Compressibility v Max Flow Gradient Primary Channel B De selects all parameter x Exits this screen Saves the settings and leaves xj Done the screen 10 34 Default resets the settings Defaut of ft File Figure 46 Method Filter screen Using Default resets the filter selection to factory settings Using File the filter settings can be stored and or stored filter setting can be loaded If a filter is set the Method screen will show the information Method name filtered The filter can be activated from the Method screen using the Toggle button If no user defined filter is in use the default filter is chosen Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Compare Methods The Compare screen is a tool that allows you to compare two methods The differences are shown in a list by displaying the values from both methods side by side You can copy parameters between the two selected methods using the Copy function w Method Compare Displayed Method INTERNAL WOLF Copy parameter left to right Quat Pump Min Pressure 2 bar OFF 5 Butosameler Copy parameter right to left Col Comp Temperature Left 20 00 C OFF DAD Slit Width 8 nm 4 nm x s 12 13 Val x Exits this screen Cancel Saves the settings and leaves 7 Done the screen Different parameters fe 15 37 Conf
114. t is no longer listed in the tree view of the Update Tool 4 Downgrade all 1100 1200 modules together to the target firmware A 05 11 13 or A 05 09 10 5 When finished the LAN RS 232 Update Tool 2 10 shows all 1100 1200 modules without the Instant Pilot the Instant Pilot shows all modules of the system in the Welcome screen 6 Close the connection and the LAN RS 232 Update Tool 2 10 Upgrade from A 05 1x to B xx 1 When the Agilent 1100 1200 series modules are on a firmware platform A 05 09 1x then update the modules to platform A 06 xx 2 Connect the Instant Pilot with A 05 1x firmware to one of the modules Gf not already done 3 After boot enter the Maintenance screen and press the Controller button Agilent Instant Pilot Users s Guide 157 5 158 Maintenance and Repair Maintenance Controller m System Controller I i iT To Update Instant Pilot A 05 xx to A 06 xx or above with Firmware Update Tool Press Transfer button to restart the Instant Pilot into it s alternate system For more information press i G Exit 12 04AM Figure 106 Update Information for firmware A 05 11 Press the Transfer button The Instant Pilot will switch into resident mode black screen and the Update Tool will list the Instant Pilot Select the Instant Pilot for update to the target firmware revision 6 Start the update When finished the Instant Pilot boots in main mode and shows
115. t or the USB Flash Drive while a firmware update is running Otherwise the module may become unusable To open the Maintenance System information press More from the Welcome screen and select Maintenance g Maintenance System L Product Serial Firmware ERICA single Immediately updates a G1311A_ DE23923124 A 06 03 001 F7 selected module or system 7 Wizard Autosampler G1329A DE91603245 A 06 02 002 C PN SN TCC G1316A DE14923865 A 06 03 001 VWD SL G1314C JP92110261 A 06 03 001 DAD SL G1315C PP00000024 B 01 03 0001 FLD G1321A DE92001563 A 06 02 001 Displays information on the available modules KS l 11 26 USB Flash Drive must be System I Controller ouat Pump Astosampier vo active Figure 96 Firmware Update Screen Simple Mode 1 Move the curser to the module you want to update 2 Press Single Agilent Instant Pilot Users s Guide 149 5 Maintenance and Repair Firmware Updates System Controller Quat Pum Autosamp Col Comp DA Det Maintenance System Confirm to continue with update Do not disconnect the cable or power Firmware Update Actions Ready to start Done G1316A uploading resident Done G1316A activating resident Starting FW Update G1316A activating main Finished cycle the modules while the update is executing The current method setpoints will b
116. the Instant Pilot Exchanging the CAN Cable CAUTION Before you open the Instant Pilot the CAN cable must be disconnected from the HPLC module to assure that no voltages are present in the Instant Pilot CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD In order to prevent damage always use an ESD protection when handling electronic boards and components 1 Place the Instant Pilot face down on a bench as shown in Figure 108 2 Carefully remove the labels that are across the screws 3 Remove the six screws that fix the rear panel Figure 108 Rear Panel location of screws 4 Remove the rear panel carefully 164 Agilent Instant Pilot Users s Guide NOTE Maintenance and Repair 5 Repairing the Instant Pilot 5 The release button 1 shown in Figure 109 may fall out during the removal Keep it Step 5 and 9 are for the OLD rear panel design only See information on Adding the Instant Pilot to an Agilent HPLC Module on page 20 The new rear panel does not have the release button NOTE Figure 109 Rear Panel location of cable connector and release button 6 Unplug the CAN cable from it s connector 2 7 Plug the new CAN cable into the connector 2 8 Fit the cable with the cable relief 3 in the front cover 9 Place the release button into its location 1 Before you replace the rear panel observe the release button mechanism at the rear panel The
117. tibility vs Modules Agilent HPLC Modules Instant Pilot Firmware Revision A 05 13 14 15 Instant Pilot Firmware Revision B 01 02 03 04 Instant Pilot Firmware Revision B 02 01 and above 1200 Infinity Series e G7104A Flexible Pump G7114B VWD e G7116B Multi Column Thermostat G7117A B DAD e G7120A High Speed Pump G7129B Vial sampler G7129A Autosampler G7162A RID G7167A B Multi Sampler 1260 Infinity Series 1220 Infinty Series LC Systems 1290 Infinity System e G4212A DAD G4220A Binary Pump G4226A Autosampler G1316C TCC 1120 Compact LC not compatible not compatible not compatible not compatible not compatible not compatible not compatible not compatible not compatible not compatible B 02 17 or above B 02 11 or above not compatible B 02 08 or above not compatible Agilent Instant Pilot Users s Guide 15 1 Start up Information Table 2 Agilent HPLC Modules Newer Series 1100 1200 modules that required special versions of Instant Pilot e G1315C DAD SL G1365C MWD SL G1315D DAD G1365D MWD e G1314D VWD e G1314E VWD SL Plus e G1367D ALS SL Plus 1100 1200 1260 ChipCube G4240A Series 1200 standard modules includes all modules not mentioned below Series 1100 standard modules includes all modules not mentioned below Pre requisites Compatibility vs Modules Instant Pilot Firmware Revision A 05 13 14 15 n
118. tics 2d Instrument Control Q ar ie System Report Figure 102 Lab Advisor System Overview 3 Select Firmware Update Then lock the system Agilent Lab Advisor Firmware Update EESO Instrument Name Progress amp 9 Lab Advisor Caution the firmware update procedure do not tum instrument off nor disconnect AC power PB System Overview Doing so may result in the instrument becoming unresponsive Configuration cs a Logs amp Results 5 61sec mm A Service amp Diagnostics Q Instrument Control Q r System Report Figure 103 Lab Advisor System Overview 154 Agilent Instant Pilot Users s Guide Maintenance and Repair 5 Firmware Updates 4 Set the correct folder for the firmware 1 Select the firmware version for the Instant Pilot Main and Resident and or the other modules Use always the latest version 2 Start the Update 3 Instrument Name Progress Caution During the firmware update procedure do not tum instrument off nor disconnect AC power Doing so may result in the instrument becoming unresponsive Device Resident Firmware Main Firmware Progress FLD A 06 50 003 A 06 55 001 DEABO03324 Keep current version x Keep curent version z G4208A 1200 Instant Pilot B 2 15 0001 B 2 15 0001 Serial DE13599999 B 02 16 0001 B 02 16 0001 Firmware files are currently taken from wadnas1 WAD Fimware Public Fimw
119. tion Preview Figure 66 Sequence Wizard Adding Calibration Information Agilent Instant Pilot Users s Guide 93 2 Working with the Instant Pilot Sequence Automating Analyses Wizard Preview Num of Injections 2 1 1 1 2 2 1 1 1 2 x 1 Cancel 7 Shows a preview of the sequence table Ly 15 57 Samples Calibration Preview Ley Es Figure 67 Sequence Wizard Preview with Calibration Parameter Multi Wizard Preview Shows a preview of the sequence table 15 56 Samples I Calibration Preview SS ae Figure 68 Sequence Wizard Preview with Calibration Parameter Alternate 94 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Saving a Sequence Sequences are stored within the Instant Pilot internal memory and or on an external USB Flash Drive The sequence is only in the controller Changes to a sequence line can be when the line is not active if sequence is running The Instant Pilot generates a list of all available sequences that can be loaded The number of sequences that can be stored depends on the number of timetable and injector program lines included In general more than 100 squinches may be stored in the Instant Pilot With differing sequence contents the actual amount of sequences to be stored may change significantly Use a USB Flash Drive to store an infinite
120. tion Mode line and change it to Injector Program in the Method view Method WOLF filtered Posttime Quaternary Pump DE23923124 0 000 ml min Injector Program Press edit to view settings __ edits the selected line Edit Control toggles between filtered and Toggle unfiltered exits the method screen Draw Speed 200 pl min Eject Speed 10 ulmin Wash Vial f Optimization None Specifies the type of injection to make Filter i Method WOLF filtered a ie a a al S a a o Quaternary Pump DE23923124 0 000 ml min OFF OFF OFF ia Hl 3 mi Fiai Autosampler DE91603245 _ Injection Volume 5 00 ul o Q D Injection Mode Injector Program Press edit to view settings Draw Speed 200 ulmin Eject Speed 10 ulmin Wash Vial Optimization None x Exit Figure 58 Method Screen Injector Program A 15 59 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Press the Default button to start with a pre defined injector program This can be modified or expanded w Method Injector Program Ea 2 _ Draw default amount from sample 3 Wait 0 02 min End of User Defined Injector Program ETTE as a ET Figure 59 Injector Program Default Program
121. to generate start requests before each run The external device starts each injection then by sending a start signal to the APG remote line That is after all the programming has been completed on the Instant Pilot and the Start button has been pressed a Start Request is issued before each run and the external device must give the Start command for the injection process Here too the module simply tracks the progress of the analysis Send multiple Detector startrequest H Injecting system Defines analysis start Wait Start a lt _ _ _ Analysis Analysis LL lt oAnalysis isiat Request gt be 5 a E O O Y nalysi a_i 2 i D pas i S lt Run Sample Figure 93 Send multiple external start request 140 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 Synchronizing Analyses with External Devices Wait for Single External Start Request After pressing the Start button the Instant Pilot waits for a single external start request on the APG remote lines When the start request is received the complete vial range or sequence is done as in standard mode under the command of the Instant Pilot without further external synchronization Sampler Remote User Starts Sequence Wait Start eon Request on Araye e Fc Wait Start Run eine Run Sample Run Sample Injecting s
122. tus Plot Spectrum DAD MWD VWD FLD Control button on various screens Agilent Instant Pilot Users s Guide 59 1 Start up Information Information on Firmware 60 Use always the latest version of the Instant Pilot firmware It is always backward compatible with all previously released versions e A 05 15 or later for firmware platform A 05xx Update main part only B 02 17 or later for firmware platform A 06 xx B 01 xx or later Update always main and resident part The firmware can be downloaded from the Agilent web http www chem agilent com _layouts agilent downloadFirmware aspx whid 69761 Agilent Instant Pilot Users s Guide G4208A Instant Pilot User s Guide 2 Working with the Instant Pilot Using a USB Flash Drive 62 Handling of Unsupported USB Flash Drive Formats 63 Printing To USB Flash Drive 65 Working with Methods 70 Loading a Method 72 Modifying a Method 73 Filtering Method Information 74 Compare Methods 75 Method Timetable 76 Method Properties 78 Saving a Method 81 Transfer of Methods 83 Offline Work on Methods 84 Import of Methods 85 Injector Program 86 Sequence Automating Analyses 91 Using the Sequence Wizard 93 Saving a Sequence 95 Sequence File Protection 96 Starting and Stopping a Sequence 98 Displaying Data Graphically 101 DAD MWD VWD FLD Spectrum 104 Connecting External Devices 107 Simultaneous Execution with Software 111 Special Functions 114 This chapter
123. ty II on page 107 The Start button on the Instant Pilot is used to start sequence analysis Standard Mode In the standard mode the analysis is under the command of the Instant Pilot The Instant Pilot issues a Start command to the autosampler as soon as all modules are ready for the next analysis The autosampler issues the Start command at the point of injection With an Agilent Autosampler integrated in the system and no external devices this is the normal mode of operation 138 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 Synchronizing Analyses with External Devices Send Single Start Request After you start the analysis with the Instant Pilot it will generate a single start request on the APG remote lines This triggers the external device which starts each injection by sending a start signal The vial range or sequence is started by the Instant Pilot but then both systems run free without further synchronization Wait Start Analysis Run Sample sR lt NoAnalysis m Ba is tl SS 30 NE lt Avnaysis _ E E Pe S Tm iO Penude z 5 i ia Injecting system defines analysis start Figure 92 Send single external start request Agilent Instant Pilot Users s Guide 139 4 Running Multiple Vial Analyses Synchronizing Analyses with External Devices Send Multiple Start Request external controlled injector This will cause the Instant Pilot
124. um Stroke AUTO Compressibility 100 10E 6 bar Max Flow Gradient 100 0 ml min Primary Channel Auto on line information in edit mode the limits are shown ci x Exit Specifies a time limit for your analysis KS 13 45 Figure 18 Method Screen The screen displays complete or filtered information about module settings and parameters of all modules Depending on the number of modules you may have to scroll through the display To change a parameter scroll down to the field and press Edit or OK Using the Toggle button switches between filtered and unfiltered view The Properties button opens the history protection of the current method see Method File Protection on page 79 Agilent Instant Pilot Users s Guide Start up Information 1 Table6 Method Functions of Keys Button Description Edit or OK lets you edit a parameter field Control opens a menu to control certain module system 1 System On Off activities depends on the connected modules ote cee cree 4 Autosampler Needle Cleaning 5 VWD SL Balance 6 DAD SL Balance 1 System Set Defaults 2 Autosampler Release Vial Toggle switches between filtered and unfiltered view Exit or Esc exits the method screen Filter used to create and edit filters Filters are stored together with the method When a filter is selected only the parameters that were selected in this filter are show
125. us all graphic files are saved separately in a folder with the name of the printout The files can be opened with a PC using Microsoft Word or Internet Explorer and printed from there The printouts have a header containing date and time see Figure 41 Agilent Instant Pilot Users s Guide 65 2 Working with the Instant Pilot Printing To USB Flash Drive Agilent Technologies SYSTEM1 04 13 07 13 07 Instrument Logbook Class Date Time Module Message B 041307 12 51 07 DAD SL Lamp off B 1307 12 51 07 DAD SL VIS lamp off 8 04 13 07 12 51 18 TCC Temperature off B 041307 12 51 18 Tec Column ID left data valid B 1307 12 51 18 frec Column ID right data valid B 41307 12 51 14 B 04 13 07 12 51 14 B 0130 12 51 18 Calibration done B azo 12 51 14 Quat Pump Pump off 04 13 07 12 51 18 TCC Valve switched to column 2 8 04 13 07 12 51 14 Quat Pump Composition ramp off B 04 13 07 12 51 14 Quat Pump Flow ramp of 8 04 13 07 12 51 04 Autosampler No service mode B 04 13 07 12 51 18 FLD Lamp off B 04 13 07 12 51 18 FLD Reference on B 130 12 51 18 FLD Calibration done B azo 12 51 04 Autosampler Initialization done Figure 41 Example of a Printed Document Instrument Logbook 66 Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 The following information can be printed Table 11 Overview of P
126. ve is connected the icon is shown as active blue Select Continue to continue the setup or Abort to close the Setup Wizard Agilent Instant Pilot Users s Guide 31 1 Start up Information 32 The next setup screens allow you to change the Date amp Time Units amp Formats and the Display Use the Direction keys for moving to the fields and Edit or OK to open the selection fields Setup Wizard Date amp Time Date amp Time Day Month Year 24 AUG k 2006 date Abort Hour Min 16 12 time rs Next valid from 1 to 31day ik 16 12 Getting Started Setup Wizard Date amp Time Figure 10 Upon startup the modules synchronize their internal clocks The clocks can also be synchronized by an external chromatographic data system like the Agilent ChemStation Agilent Instant Pilot Users s Guide Start up Information 1 Configure Setup Wizard Instrument Name EEB The instrument name is a user defined Instrument Name alias for the instrument It is shown in the titel of the welcome screen and on printouts to identify the instrument SYSTEM1 System name can be entered JEnterupto20characters Mw 16 26 Figure 11 Getting Started Setting a System Name A system name will be displayed on screens and printouts as identifier Setup Wizard Units amp Formats Units amp Formats Time A 24 12h Date Month Month Day Year m Year y Month D
127. y Wizard and Save buttons are disabled e There is no Import functionality To transfer sequences from one LC system to another you can use the Instant Pilot or a USB Flash Drive Agilent Instant Pilot Users s Guide Working with the Instant Pilot 2 Tray View The current sequence s status is shown graphically The sequence samples are shown at their locations on the tray using colors representing their states Green already processed sample Blue sample to process Magenta calibration sample Yellow aborted sample The current sample is colored alternating blue sample or magenta calibration sample and light blue The currently processed sample location and the method name are also shown textual on the left The bar graph at the bottom shows the sequence state and overall sequence time P1 A 12 METH_101 OOO OO reine sigs 0070010100701000 tetotatatetatete E E E E ERRER oho ml 12 49 SS EE S E Figure 71 Sequence Tray View Agilent Instant Pilot Users s Guide 97 2 98 Working with the Instant Pilot Starting and Stopping a Sequence When you press Start the Start Analysis dialog pops up where you can select between e setting up a sample range e starting the current saved sequence or e resuming a paused sequence e using a blank run e start from selected line If no sequence is currently paused this functions is disabled The sa
128. ystem defines analysis start i Run Sample i Figure 94 Wait for single external start request Agilent Instant Pilot Users s Guide 141 4 Running Multiple Vial Analyses Synchronizing Analyses with External Devices Wait for Multiple Start Request Instant Pilot controls injector After pressing the Start button the Instant Pilot waits for an external start requests before injection or sequence The start requests have to be generated by the external device This mode is recommended when a device needs extra time to get ready for the next analysis and thus has to be in charge of the start event The receive of a Start Request leads first to a Prepeare e g balance of a detector and afterwards to an injection by the sampler who then sends the Start via APG remote PEIE SEEEN Instant Pilot ls i i Wait multiple Sampler f __start request S S Sinan User Starts i Wait Start Request _ A a ster Request Eoo Aayo aa Run Sample o gt 3 lt 2 a Sample Sample Wait Start a Request eo Request gt Start Analysis gt ame nalysis ib 12 Injecting system defines analysis start Run Sample Figure 95 Wait for repeated external start request 142 Agilent Instant Pilot Users s Guide Running Multiple Vial Analyses 4 An Agilent variable wavelength detector or diode array detector will perform a balance assumed that Aut

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