LHO0731_Lot A10886 - Thermo Fisher Scientific
Contents
1. patents 6 046 807 For Research Use Only CAUTION Not for human or animal therapeutic or diagnostic use Invitrogen Corporation 542 Flynn Rd Camarillo e CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PILHO0731 Rev 04 10 Page 2 of 2 DCC 10 1340 Important Licensing Information These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or our website www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses Unless otherwise indicated these products are for research use only and are not intended for human or animal diagnostic therapeutic or commercial use2. Cell Extraction Buffer FNNOOI1 Lysates were either treated with Sample Treatment Buffer 2500 STB or the Sample Treatment Buffer incubation step was omitted an STB For the treatment 5 uL of Jurkat cell lysate was diluted with e 5 uL of Sample Treatment Buffer ST001 and incubated on ice for 20 S bwa minutes After incubation 40 uL of Assay Diluent DA04 was added 1000 F to the treated sample 500 04 STB STB Explanation of symbols Symbol Description Symbol Description Catalogue Number Batch code RUG Research Use Only vo In vitro diagnostic medical device x Use by Temperature limitation inal Manufacturer EC REP European Community authorised representative Without does not contain With contains een A Protect from light AP Consult accompanying documents ghd a li Directs the user to consult instructions for use IFU accompanying the product By purchasing this Kit which contains fluorescently labeled microsphere beads authorized by Luminex Corporation Luminex you the customer acquire the right under Luminex s patent rights to use this Kit or any portion of this Kit including without limitation the microsphere beads contained herein only with Luminex s laser based fluorescent analytical test instrumentation marketed under the name Luminex 100 or 200 This product is covered by one or more of the following U S
3. Glycogen Synthase pSpS641 645 Singleplex Bead Kit Technical Data Sheet Catalog LHOO0731 Lot A10886 invitrogen Note A letter at the end of the lot number signifies an additional packaging of this same lot Intended Use This kit is comprised of components for the measurement of Glycogen Synthase pSpS641 645 in cell lysates Buffer reagents needed to complete the reaction are sold separately under Catalog LHB0002 These reagents are intended for use in the Luminex 100 or 200 System only Note This kit uses a Co Incubation Assay format Refer to the User Manual included in the Intracellular Protein Buffer Reagent Kit Cat LHB0002 for further information Before attempting to multiplex this kit with any other kits please contact Technical Support This kit is configured for research use only and is not to be used in diagnostic procedures Reagents Provided Glycogen Synth pSpS641 645 1 Part LM200 Description Antibody Bead Concentrate 10X Lot A10883 Size 0 25 mL 100 tests Bead Region 20 Form 0 25 mL 10X bead concentrate solution in storage buffer Contains 0 05 sodium azide as a preservative Storage Light sensitive material Store at 2 to 8 C in the dark until the expiration date indicated on the kit Glycogen Synth pSpS641 645 2 Part DN200 Description Detection Ab Concentrate 10X Lot A10884 Size 0 5 mL 100 tests Form 0 5 mL of a 10x stock of Detection Antibody Concentrate Co
4. alibrated to its respective Invitrogen ELISA kit Glycogen Synthase pSpS641 645 ELISA KHO0731 Subsequent lots of standard will be normalized to this lot of material to allow consistency of quantitation Species Reactivity This reagent set recognizes human mouse and rat proteins Other species have not been tested For Research Use Only CAUTION Not for human or animal therapeutic or diagnostic use Invitrogen Corporation 542 Flynn Rd Camarillo e CA 93012 Tel 800 955 6288 E mail techsupport invitrogen com PILHO0731 Rev 04 10 Page 1 of 2 DCC 10 1340 Important Licensing Information These products may be covered by one or more Limited Use Label Licenses see the Invitrogen Catalog or our website www invitrogen com By use of these products you accept the terms and conditions of all applicable Limited Use Label Licenses Unless otherwise indicated these products are for research use only and are not intended for human or animal diagnostic therapeutic or commercial use Glycogen Synthase pSpS641 645 Singleplex Bead Kit Technical Data Sheet Performance Characteristics Typical Standard Curve Glycogen Synthase pSpS641 645 Recovery 10 FNNO0011 Analytical Sensitivity The analytical sensitivity was determined by adding two standard deviations to the median fluorescence intensity obtained when the zero standard was assayed 32 times Recovery To evaluate recovery analytes were spiked at 3 different concentratio
5. ns into 10 Cell Extraction Buffer Cat FNNOO11 and 10 NP40 Cell Lysis Buffer Cat FNN0021 Units mL Note This is a representative standard curve Absolute MFI values will vary Sample Preparation This kit has been validated with cell lysates prepared in Cell Extraction Buffer Cat FNN0011 and NP40 Cell Lysis Buffer Cat FNN0021 Optimization of cell stimulation and cell lysis procedures may be required for each specific application Refer to the User Manual included in the Buffer Reagent Kit for further information Example Data To evaluate the performance of this kit natural Parallelism Glycogen Synthase pSpS641 645 Glycogen Synthase pSpS641 645 from Jurkat cell lysate was serially diluted in Assay Diluent The median fluorescence intensity of each nual dilution was plotted against the Glycogen Synthase pSpS641 645 S000 standard curve Parallelism is presented in the figure to the right and 4000 indicates that the recombinant standard accurately reflects Glycogen E shan Synthase pSpS641 645 content in cell lysate o Jurkat 2000 m Standard 1000 0 T T T 1 0 20 40 60 80 Units mL Effect of Sample Treatment Buffer on Glycogen Synthase pSpS641 645 Signal in Cell Lysate 3000 5 The data presented to the right demonstrate the impact of the Sample Treatment Buffer pre incubation step on the observed signal In this study Jurkat cells were lysed in
6. ntains 0 1 sodium azide as preservative Concentration of antibody is matched to this lot of beads Do not mix lots of Coated Beads and Detection Antibody Storage Store at 2 to 8 C until the expiration date indicated on the kit Glycogen Synth pSpS641 645 3 Part SM200 Description Standard Lot A10885 Size 2 Vials Form This standard lyophilized recombinant protein is designated in Units mL The protein in this standard has been calibrated with its respective Invitrogen ELISA kit Detailed information on calibration is provided below Contains 0 1 sodium azide as a preservative Storage Store at 2 to 8 C Use within 1 hour after reconstitution The remaining reconstituted standard should be discarded or frozen in aliquots at 80 C for further use The standard can be frozen and thawed one time without loss of immunoreactivity Concentration of Reconstituted Standards Glycogen Synthase pSpS641 645 150 Units mL Tmportant note The concentrations of reconstituted standards are lot specific Please verify all concentration values entered in data analysis software Reconstitution Reconstitute in 1 0 mL Assay Diluent Recommended Starting Concentration for Standard Curve Upon reconstitution the starting concentration of standards is the value cited above Make 2 fold serial dilutions in Assay Diluent Refer to the User Manual included in the Buffer Reagent Kit for further information Calibration This reagent set was c
Download Pdf Manuals
Related Search