DOUBLE HEAD PERISTALTIC PUMP
Contents
1. 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 14 DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL Instructions for use Identification of Operating Controls Pump Control graduated from 0 to 600 ml min Power Inlet Main Switch Signalitic board Procedure for Setting Start the cooling system wait until the water Is at expected temperature Consult cooling system s manual if necessary Rough water interferes in sonication phenomenon Bioruptors sonication bath does not allow water movement upper than 500 ml min Income water flow cold flow should be adjusted around 450 ml ml The back flow warm flow must be at least the same of the income flow Note The drawing flow cannot be lower than the discharging flow otherwise the volume of water in bath would increase upper to the limit On the other hand the warm flow is able to be higher than the cold flow because once the water upper the level is drawn pumps will draw air which is not a problem Once the flows adjusted you don t need to change them just switch off the main switch Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL PAGE 15 Equipment maintenance Note Before starting maintenance work turn the main swi2. Suction Height 1 9 meter Tube size inch 1 4 Inner Diameter x 3 8 Outer Diameter Tube size mm 6 35 Inner Diameter x 9 525 Outer Diameter Tube matter Pharmed Silicone Fluran Tygon LFL Input Rated voltage Vin 100 240 V AC Voltage range 85 264 V AC Line frequency range 47 63 Hz Rated current lin 0 7 0 35 A Double fused 1 A Internal circuits Rated voltage V 24 V DC Rated current 1 3 Current limitation 2 A Note In case of infection the entire water circulation loop tubing can be sterilised by autoclave or ethylene oxide as well as by gamma radiation Environmental Conditions Devices are designed to be safe under the following conditions e indoor use e altitude up to 2 000 m e operating external temperature 0 C to 40 C e fluid temperature 4 C to 50 C e maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C without condensation e MAINS supply voltage from 100V AC to 230V AC with fluctuations up to 10 of the nominal voltage e transient overvoltages typically present on the MAINS supply e Degree of protection IP20 e POLLUTION DEGREE 2 Normally only non conductive POLLUTION occurs Occasionally however a temporary conductivity caused by condensation is expected Warning Never use in atmosphere with flammable gas Pump motors may generate sparkles and may light fire or explosion in atmosphere with flammable gas Never use in any
3. location where there is a possibility of extreme dust Environment exempt from sunlight is required Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL PAGE 7 Equipment installation In the interest of safety this equipment must only be installed by competent personnel after read and understood this section and considered any hazard involved Even though no particular hazards have been identified during this installation Before starting installation work turn the main switch off beyond power connection and secure the unit against being re energized No special tools required Installation shown below is performed on a NESLAB RTE Bath Circulator Location Requirements e Never install this equipment in a place where Environmental Conditions mentioned above are infringed e 3 meters square needed to set up the whole equipment e Distance between chiller and sonication baths should not be more than 1 5 meters vertically or horizontally e Additional power plugs including earthing terminal needed for the pumps Global scheme a Connection to Bioruptor one waterbath y DI R Water Reservoir Chiller Double Peristaltic Pump Bioruptor Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE
4. not usable This equipment can be used to connect two Sonication Baths to a Water Reservoir Chiller This equipment is designed to be used for the above described application only Any medical application is out of scope Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL PAGE 5 Equipment Components Component Comments Quantity 1 amp 2 Double head peristaltic pump 2 3 Plastic Nozzle Connector 2X2 4 Tie Wraps 10 4 Small Tie Wraps 4 5 Sturdy Tubing Inner diameter 6 35 mm Outer diameter 9 25 mm PharMed BPT Sa Life time expected 1 000 hours 6 Silicon Tubing Inner diameter 8 mm Outer diameter 12 mm 6 m Transparent 7 Tube Holders Composed of two parts one rigid plastic side T0 one gluing side 8 Black Plastic Connector 2 Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 6 DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL Technical Specifications Item Description Dimensions 100 x 200 x 250 mm Weight 2 128 kg Motor type DC Geared Motor 8 4 VDC RPM 0 210 round per minute Discharge rate 0 610 ml per minute Discharge pressure 0 1 MegaPascal
5. of the connecting tool which is connected to warm flow This piece of tubing will determine the level of water in the Bioruptor s tank as the circulator is sucking the water from this opening This will be done later when the circulator is filled with water The tubing can be cut gradually with a pair of scissors to allow a progressively higher level of water The required level is determined by the blue line water level in the tank Connect a second piece of silicone tube with the lower part of the connecting tool which is connected to cold flow Cut a piece long enough to allow the water flow going into the water For the connection of a second sonication watherbath Bioruptor Twin please proceed exactly the same way Right at the surface Bioruptor Sonication Bath Option 2 Place 2 black plastic connectors on the motor plate standing on the waterbath as shown on picture below Screw the plastic nut on the thread over the motor plate Do it for both holes and set the motor plate on the top of the waterbath Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL PAGE 13 Then connect the pipes to the Bioruptor waterbath Insert the silicon 6 x 9 mm pipes in the black plastic connectors Diagenode Inc North America Phone
6. the product for a period of one year starting from the date it was delivered In case of repair or replacement on a product under warranty expenses will be at Diagenode s charge including any costs required to return the repaired or replacement product to you This warranty covers only manufacturing defects and does not cover any damage caused by misuse non respect of recommendations described in this manual neglect accidents abrasion exposure to extreme temperatures solvents acids We strongly recommend that maintenances or repairs on your product are performed by our approved Diagenode service center Improper or incorrectly performed maintenance or repairs void this warranty Intended use of the equipment The pump device as well as all components from this parcel including plastic nozzle connectors tubing strips and tube holders can be used as a junction between any Water Reservoir Chiller min specification mentioned below and a Bioruptor waterbath Bioruptor one waterbath Bioruptor Twin two waterbaths Any brand any type Bath circulator immersion cooler of Water Reservoir Chiller is suitable as long as the capacity of the reservoir is 4 liters min the reservoir allows insertion of hoses the cooling power is 500 Watt min at external temperature of 20 C the cooling temperature range reaches 4 C 3 C Warning Recirculating Chiller device close loop without possibility of water reservoir is
7. 00 Liege Belgium Tel 32 4 364 20 50 an 2 4 364205 ordersediagenode com infoediagenode com Diagenode Inc USA NORTH AMERICA 400 Morris Avenue Suite 101 Denville NJ 07834 USA Tel 1 862 209 4680 Fax 1 862 209 4681 orders naediagenode com info naediagenode com For a complete listing of Diagenode s international distributors visit http www diagenode com pages distributors html For rest of the world please contact Diagenode s a 2010 Diagenode Inc All rights reserved The content of this document cannot be reproduced without prior permission of the authors Bioruptor and IP Star are registered trademarks of Diagenode Inc
8. 8 DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL b Connection to Bioruptor Twin two waterbathes E E Water Reservoir Chiller YN Double Peristaltic Pump Double Peristaltic Pump Bioruptor Bioruptor Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL PAGE 9 Cool Water Device Installation cat BioAcc Cool Read carefully Installation manual from Cool Device and perform as mentioned Note Some kind of Water Reservoir Chillers as so called Cold Bath Circulators include pumps they might not be appropriate for your use Switch them off if possible If there is no switch off selection water flow has to be turned into a continuous loop by any means See pictures below as examples Water outwards Nozzles Use spare Tubing 8mm I D x 12mm O D to set a continuous loop up Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 10 DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL Pump Device Installation cat TWi PUMP Two pumps per apparatus one is dedicated to the water flow going from the Water Reservoir Chiller to the Sonication Water Bath called cold flow the other one is dedicated to the water flow going from the sonication water bath to the chil
9. O CONO aaea e ia aaao pa SA 14 PROC gt o III 14 EGUIPIMEBEMIGIDIGNANO ee eee ee eke ae heat ewe los eee a a 15 Maintenance and INSPEC vrs os de mi Bios dae Che owes ia e ei a oe 15 siii ele phoned oe eee nore ares enue leto oe eee so dos da 15 Technica ASSISItANCO autre ah a BS es wy WPI a ses Be eel lg eh ae de JD I Hm eG 15 Ordering Informat OD En a an sia sab Back Cover PAGE 3 Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 4 DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL Introduction Warnings These instructions cannot claim to cover all details of possible equipment variations nor in particular can they provide for every possible example of installation or operation Trouble free and safe operation of the unit is dependent on proper transport and installation by qualified personnel Guarantee Limited one year global warranty Diagenode guarantees its products against possible manufacturing defects in material and workmanship Diagenode products are rigorously tested to ensure that the products you trust meet stringent standards Consequently if a problem occurs with a Diagenode product and the problem is caused by manufacturing defects in material and workmanship Diagenode will in its discretion either fix or replace the product in accordance with the warranty terms and conditions stated herein The warranty applies only to the first purchaser of
10. diagendte Innovating Epigenetic Solutions DOUBLE HEAD PERISTALTIC PUMP Cat No B02030001 TWI PUMP Version 6 08 11 10 agenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com Technical Assistance amp Ordering Information Diagenode s a BELGIUM EUROPE Diagenode Inc USA NORTH AMERICA Avenue de Uhopital 1 400 Morris Avenue Suite 101 lour GIGA 3rd Floor Denville NJ 07834 USA 4000 Li ge Belgium Tel 1 862 209 4680 Tel 32 4 364 20 50 Fax 1 862 209 4681 Fax 32 4 364 20 51 techsupport na diagenode com techsupport diagenode com orders na diagenode com orders diagenode com For a complete listing of Diagenode s international distributors visit http www diagenode com en support distributors php For the rest of the world please contact Diagenode s a DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL Contents INIFOGUELO ea ada a daa a ee ey nier 4 WAI acer oe ade des EROE e E ea oo ee oe aaa debes na dada eee 4 o II SS 4 intended use OF MS EQUIPO cerrarse is pa a a AE E Dee dio ns 4 OCP Wen E NON N a E E onde ni 5 sige Eo o a de de nd EE 6 Environmental Conditions isernia ao de dir ta 6 EGQUIDMECH AIME EAN de do taneoni 7 Bas Oy eG DS ANS o TE I elen E n Eire ee a ec I Cool Water Device Installation eee eee ee eee 8 o ls eli ss ee ue oh Sie nergy Se er rs to de tue dl meer uote 9 oia ER O ai a 14 SAISON or oper T
11. ler water bath called warm flow Cut 2 pieces of silicon tubing one for each flow Cut them long enough especially if using the soundproof box Glue four tube holders on chiller water reservoir Two for the warm flow and two for the cold flow as shown on pictures Warm flow pipe must discharge close to the cooling coll from the chiller water reservoir Cold flow must draw water far away from the warm flow pipe discharge This Is to prevent the cold flow pipe drawing warm water Tube holders Water reservoir At least two plastic holders per pipe are required to fix appropriately the pipe because it moves constantly due to peristaltic pump head movement Before gluing plastic tube holders make sure the surface is perfectly dry otherwise it does not glue Gluing them while the reservoir is completely unfilled and dry is advised Note submerge the pipe into the cold water reservoir the end of the pipe must be one cm above the bottom of the tank Check the pipe in order to prevent any squeezed bends as it could diminish flow proprieties Now insert the 2 pipes into the 2 heads from the pump Follow the below steps Europe Diagenode sa CHU Tour GIGA B34 3 tage Avenue de l H pital 1 4000 Li ge Sart Tilman Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL PAGE 11 Put sturdy tubing in plastic cassette turn each cassette cou
12. nter of clockwise until they stops then pull them out Add grease to the cassette shafts and the surface of new tubing Put a piece of sturdy tubing in between rotor and cassette as shown on picture below Place back the cassette to the base Do the same for the other pump head a gt N Knot is installed inside of the guide Cut with scissors the silicon tube standing for the warm and cold flow where you want to integrate the pump Then connect each silicon tube ending to the appropriate sturdy tube ending Watch direction of flow each pump has a drawing side and a discharging side Heads spin clockwise which means left nozzles are the discharging side and right ones are the drawing side Have a look on the global scheme page 7 to make sure Place plastic pipe holders to fix pipes along Concerning the upper lid ofthe Bioruptor s bath set two plastic nozzles over the upper part of the sonication bath Press firmly to ensure fixation Option 1 Connect the warm flow pipe and the cold flow pipe from the sonication water reservoir to the chiller water reservoir gi AC D Plastic tube holders Upper part of the sonication bath AS Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 12 DIAGENODE DOUBLE HEAD PERISTALTIC PUMP USER MANUAL Connect a piece of silicone tube with the lower part
13. tch off beyond power connection and secure the unit against being re energized Maintenance and Inspection Replace hoses if you notice any leakage If you notice any abnormalities with the speed of flow check it using a graduated beaker and a timer Fuse replacement Unlock the fuse drawer with a narrow blade screwdriver Info F 1A L 250 Vac Fuse reference Storage Pour water out of tubing and remove it off of pump mechanism when pumps will not be used for a long time The tubing wall may be damaged if the rollers compress the same part of the tubing for a long time Technical assistance Gilles Ansay Sonisation Products Phone 32 4 364 20 69 Fax 32 4 364 20 51 gilles ansay diagenode com http www diagenode com _10 _08_11 MA Pump V6 Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com Ordering information Description Cat No Format Bioruptor Twin sonication device with 1 5 UCD 400 TM 1 unit ml tube holder new soundproof box Bioruptor Twin sonication device with 1 5 UCD 400 TO 1 unit 8 19 ml tube holder amp new soundproof box Visit us at one of Diagenode s demo sites or discover our Automated Systems by performing some assays with the help of our R amp D and Technical Department www diagenode com diagengte Innovating Epigenetic Solutions Diagenode s a BELGIUM EUROPE Avenue de l h pital 1 Vou GG sive Flo 40
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