Class II, Type B2 (Total Exhaust) Biological Safety Cabinets
Contents
1. 1610 mm 63 3 Esca y OOO F 1540 mm 60 6 20 0 970 mm 1270 mm 1570 mm 1870 mm 2440 m 6 2 50 0 7 61 8 73 6 7 96 0 1115 mm 1420 mm 1725 mm 2030 mm 2600 mm 43 9 55 9 67 9 79 9 102 4 180mm 7 1 15 Exhaust collar Exhaust HEPA filter Blower Downflow ULPA filter angled Downflow Sensor Standard UV light Retrofit Kit provision Single piece stainless steel work tray Pressure Switch Port Exhaust Sensor Electrical Electronic panel Fluorescent lamp Standard IV bar Retrofit Kit provision Maximum load for IV bars is 1 kg Service Fixture Retrofit Kit provisions 2 on Each Side Electrical outlet Retrofit Kit provision 0 9 meter 3 model one single outlet in workzone 1 2 1 5 1 8 and 2 4 meter 4 5 6 amp 8 models two single outlets in workzone 15 Stainless Steel Armrest With Groove 16 Drain valve Retrofi2. 11 ART Equipment Biological Safety Cabinets CO Incubators Compounding Pharmacy Equipment Containment Pharma Products Ductless Fume Hoods Lab Animal Research Products Laboratory Fume Hoods Laboratory Ovens and Incubators Laminar Flow Clean Benches PCR Cabinets PCR Thermal Cyclers Powder Weighing Balance Enclosures Ultra low Freezers Since 1978 Esco has emerged as a leader in the development of controlled environment laboratory and pharmaceutical equipment solutions Products sold in more than 100 countries include biological safety cabinets compounding pharmacy equipment containment pharma products ductless fume hoods in vitro fertilization workstations lab animal research products laboratory fume hoods laboratory ovens and incubators laminar flow clean benches and PCR products and instrumentation With the most extensive product line in the industry Esco has passed more tests in more languages for more certifications throughout more countries than any biosafety cabinet manufacturer in the world Esco remains dedicated to delivering innovative solutions for the clinical life science research and industrial laboratory community www escoglobal com Life Science Chemical Research e Assisted Reproductive Technology ART Pharmaceutical Equipment General Equipement ESCO WORLD CLASS WORLDWIDE A4_vC_07 Jan 2013 Esco Technologies Inc e 2940 Turnpike Drive Units 15 16 e Hatboro PA 19040
3. b Labculture Labculture A 4 FO O y Labculture Class Il Type B2 Total Exhaust Biological Safety Cabinet Model LB2 4B_ Class Il Type B2 Total Exhaust Biological Safety Cabinets The Safety Solution for Life Science Laboratories kan in uickstart mode to turn the blower on when the sash window is moved fromm fully closed position and light on off when the sash window reaches safe sash height bculture b Lower exhaust volume and pressure to save energy b Lower noise for better working comfort gt Built in exhaust pressure switch for fast and accurate alarm switching gt Dual airflow sensors to measure exhaust volume and gt L VEN D Esco Sentinel Gold controller with large LCD that simultaneously display key parameters EW Simplified field calibration mode for easier airflow sensor calibration VEWACIIET free contacts to trigger external alarm external blower damper As gt Built in RS 232 port for remote monitoring of cabinet operation Total exhaust feature allowing the cabinet to be used for work with volatile chemicals and radionuclides used as adjuncts to microbiological studies Unique Esco Dynamic Chamber plenum Enhanced Auto Purge side capture zones optimize containment Long life supply ULPA filter per IEST RP CC001 with gt 99 999 efficiency for particle size between 0 1 to 0 3 microns Angled supply filter matches cabine
4. e Each cabinet is shipped with a comprehensive user s manual complete with a report documenting all test procedures e Additional IQ OQ documentation is available upon request e Contact your local Sales Representative for specific warranty details or docu mentation requests Accessories and Options Esco offers a variety of options and accessories to meet local applications Contact Esco or your local Sales Represen tative for ordering information Support Stands e Fixed height available 711 mm 28 or 864 mm 34 With leveling feet 38 1 mm 1 5 With casters e Telescoping height stand for leveling feet nominal range 660 mm or 6 e The 10 angled viewing window and u u v cia of this page for the full list 960 mm 26 or 37 8 narrow profile front grille improves f e Contact Esco or your Sales Representative Telescoping height stand for casters reach into the work area aa ma The Jeli 7 jahti for site preparation information see nominal range 660 mm or 880 mm e The lamp delivers uniform lightin are n f p gnung Electrical Specifications 26 or 34 6 to the work surface for greater Adjustable in 25 4 mm 1 increments comfort reduced glare and E O DA ei Warranty e Cradle stand electrical hydraulic Pp ne P 4 y The Labculture cabinet is warranted for infinitely adjustable with casters pociicauong 4 years excluding consumable parts and Elevates to seating or standing e The front a
5. microbiological work involving volatile chemicals and radionuclides The non recirculating design guarantees safety in cases where exhaust HEPA filtration fails to ensure containment Note provided exhaust duct connection is air tight Inflow of room air enters the front air grille to establish operator protection room air does not enter the work zone preventing product contamination The front grille has proportionally larger perforation on the extreme left and right side Combined with the side air gap between the work tray and the side wall the stronger side capture zones in crease protection in this critical area The inflow grille has a convex shape to maintain operator protection by discouraging users from placing objects on it Raised armrest prevents the likelihood of the inflow grille being blocked by the operator s arms Auto purge openings located at the front side walls eliminate eddy currents and dead air pockets in the critical area behind the sash window The ULPA downflow supply filter is positioned proportionally to the cabinet front angle to direct more air forward to the front air grille The inflow velocity downflow velocity air flow path and intake geometry are precision tuned and tested to create an optimum air curtain on the front opening this curtain maintains personal and product protection by increasing barrier protection Please note that Type B2 cabinet must be equipped with e
6. Cabinet Design Construction Cleaning 18 The cabinet shall be of triple wall design whereby all positive pressure plenums capable of handling contaminated air shall be surrounded by negative pressure No positive pressure areas shall be accessible external to the cabinet The third wall shall conceal utilities 19 The cabinet shall maintain containment performance even when removable work area components are removed for cleaning 20 The work tray shall be one piece removable stainless steel with radius corners without crevices or joints 21 The cabinet shall have a stainless steel one piece fabricated drain trough with open angles to channel spills to acommon drain 22 The closed sidewall shall be sealed without perforations return air slots or concealed areas which can contain contaminants 23 The cabinet shall be free of sharp edges non functional protrusions bolts screws or hardware and all metal edges shall be deburred Ergonomics and Convenience 24 The front sash shall be frameless to maximize visibility and accessible for cleaning front and back Sash glass shall be safety glass 25 The sash counterbalance shall be suspended on two high strength cables and the sash shall lock into position in the event one cable becomes detached 26 Magnetic not mechanical proximity sensors shall work in conjunction with the control system to indicate proper sash position for containment 27 Fluorescent lamps s
7. Target agar plates are placed throughout the entire work surface e Acceptance The number of Bacillus Subtilis CFU recovered from the agar plates shall not exceed 5 CFU per test Cross Contamination Test The test objective is to evaluate cabinet protection from cross contamination of samples placed simultaneously inside the workzone e Anebulizer containing 55 mL of spores 5 to 8 x 10 mL is placed against one of the workzone sidewalls e Target agar plates are placed 36 cm 14 inches away from the same side wall as the nebulizer e Acceptance The number of Bacillus Subtilis CFU recovered on agar plates shall not exceed 2 CFU per test HPV Test Compliant Safer Hydrogen Peroxide Vapor Decontamination Compatibility Esco biological safety cabinets are Hydrogen Peroxide Vapor HPV compliant and decontaminatable cabinets tested with both BIOQUELL and STERIS patented processes personnel product and the environment downflow velocity expected dy High Deswndlow WAN 0 50 mys HPV is a safer and more efficient alternative to conventional decontamination using formaldehyde CH 0 e HPV is non carcinogenic and odorless while formaldehyde is carcinogenic toxic and has pungent smell e HPV is more effective and rapid against biological organisms compared to formaldehyde e HPV requires approximately 4 7 hours for set up decontamination and tear down compared to a total of 12 15 hours needed to
8. and static pressure at the connection point note the Required CBV Exhaust Volume and NSF required Static Pressure listed on Anti Blowback Valve Optional Note Cabinet shown with optional support stand optional air tight optional anti blowback valve and optional exhaust blower Exhaust ductwork is not provided 3 Pitot Tube Duct Traverse Penetration Drill Penetrations in Ductwork y 4 lt q en isoce Labculture Exhaust Blower at Terminal End Preferably one blower per individual cabinet and Connected to Emergency Powe Supply Air Make Up for Downflow and Inflow Air Located far away from exhaust blower Page 8 Remember to add the pressure 4 Ensure the lab is supplied with sufficient makeup air 5 Provide an air tight damper mandatory and anti blowback valve optional 6 Provide provisions for pitot tube duct traverse These can be drilled in the ductwork on site loss for the specified airflow volume in the ductwork A dedicated exhaust system one exhaust blower to one cabinet Is strongly recommended It is also recommended the exhaust system be operated continuously 7 Observe Esco recommendations on suitable cabinet location especially putting the cabinet away from external airflow disturbances 8 Certify cabinet after installation before use ESEO WORLD CLASS WORLDWIDE
9. 70 x 623 x 715 mm 50 0 x 24 5 x 28 1 1570 x 623 x 715 mm 61 8 x 24 5 x 28 1 1870 x 623 x 715 mm 73 6 X 24 5 x 28 1 2440 x 623 x 715mm 96 0 x 24 5 x 28 1 Note Must use this for HVAC sizing Minimum exhaust static pressure for clean exhaust filter 400 Pa 1 6 in H O 375 Pa 1 5 in H 0 375 Pa 1 5 in H 0 400 Pa 1 6 in H O 0 45 m 4 8 sq ft 0 6 m 6 5 sq ft 0 75 m 8 1 sq ft 0 9 m 9 7 sq ft 1 2 m 13 sq ft 203 mm 8 0 203 mm 8 0 203 mm 8 0 203 mm 8 0 203 mm 8 0 Inflow 0 53 m s 105 fom Downflow 0 31 m s 60 fpm Inflow 376 m h 223 cfm 492 m h 292 cfm 608 m h 361 cfm 724 m h 429 cfm 945 m h 560 cfm Downflow 628 m h 363 cfm 822 m h 476 cfm 1016 m h 588 cfm 1210 m h 700 cfm 1580 m h 914 cfm Concurrent Balance Value CBV Exhaust Volume at cor responding Static Pressure 1127 m h 658 cfm 1476 m h 862 cfm 1824 m h 1065 cfm 2173 m h 1269 cfm 2835 m h 1656 cfm 475 Pa 1 9 in H O CBV Static Pressure with additional 174 Pa 0 7 in H O required by NSF ANSI 49 2010 Note Must use this for HVAC sizing 575 Pa 2 3 in H 0 550 Pa 2 2 in H O 550 Pa 2 2 in H 0 575 Pa 2 3 in H 0 650 Pa 2 6 in H 0 gt 99 999 for particle size between 0 1 to 0 3 microns gt 99 99 at 0 3 microns 460 mm 18 2 Opening NSF ANS
10. A filter efficiency shall be gt 99 999 for 0 1 to 0 3 microns Typical HEPA Filter shall be gt 99 99 at 0 3 microns 9 An integral filter guard shall be affixed to prevent damage to the filter media 10 The filters shall be a individually scan tested by the manufacturer b individually scan tested after assembly and b easily accessible for scan testing in situ by means of a dedicated upstream sampling port accessible from within the cabinet 11 The supply filter shall be angled and oriented to the 10 cabinet front angle to maximize downflow uniformity over the work surface 12 A removable perforated metal diffuser shall be installed below the supply filter to optimize airflow uniformity and to protect from damage Blower System 13 The cabinet shall have a direct drive permanently lubricated centrifugal blower motor dynamically balanced in two planes compliant to ISO 2710 for low noise low vibration and long filter life 14 The blower motor shall have an external rotor design and include an automatic thermal cut out to disable the motor in case of overheating 15 The blower motor shall compensate for filter loading 16 The blower motor system shall be enclosed within a dynamic chamber shaped steel plenum and integrated with the removable supply filter assembly to simplify filter changing 17 The cabinet shall be equipped with an integral exhaust collar for connection to the laboratory ducting system
11. I 49 57 58 59 60 61 EN 12469 54 55 56 57 58 A At Zero Ambi gt 1250 Lux gt 1400 Lux gt 1200 Lux gt 1200 Lux gt 1200 Lux gt 116 foot candles gt 130 foot candles gt 111 foot candles gt 111 foot candles gt 111 foot candles Main Body Electro galvanized steel with white oven baked epoxy polyester Isocide antimicrobial powder coated finish Work Zone Stainless steel Type 304 with No 4 finish 220 240V AC 50Hz 10 LB2 3B1 LB2 4B1 LB2 5B1 LB2 6B1 LB2 8B1 110 120V AC 60Hz 10 LB2 3B2 LB2 4B2 LB2 5B2 LB2 6B2 LB2 8B2 220 240V AC 60Hz 10 LB2 3B3 LB2 4B3 LB2 5B3 LB2 6B3 LB2 8B3 279 kg 615 Ibs 317 kg 699 Ibs 359 kg 791 Ibs 438 kg 966 Ibs 591 kg 1304 Ibs 318 kg 703 Ibs 370 kg 814 Ibs 402 kg 886 lbs 491 kg 1083 Ibs 651 kg 1435 Ibs 1210 x 950 x 1950 mm 47 6 x 37 4 x 76 8 1520 x 950 x 1950 mm 59 8 x 37 4 x 76 8 1900 x 950 x 1950 mm 74 8 x 37 4 x 76 8 2150 x 950 x 1950 mm 84 7 x 37 4 x 76 8 2720 x 950 x 1950 mm 107 0 x 37 4 x 76 8 2 24 m 79 1 cu ft 2 82 m 99 6 cu ft 3 52 m 124 3 cu ft 3 98 m 140 6 cu ft Height includes exhaust collar and depth includes the remove able arm rest and front cover When they are removed depth is 790 mm 31 1 This Concurrent Balance Value CBV Exhaust Volume per Pitot Duct Traverse and Static Pressure at cabinet exhaust connection should be used when sizing the HVAC exhaust am
12. USA Toll Free USA and Canada 877 479 3726 e Tel 215 441 9661 Fax 215 441 9660 us escoglobal com usa escoglobal com t_LB2 G2_Brochure Esco Micro Pte Ltd e 21 Changi South Street 1 Singapore 486 777 Tel 65 6542 0833 Fax 65 6542 6920 e mail escoglobal com www escoglobal com gical Safety Cabine Esco can accept no responsibility for possible errors in catalogues brochures and other printed materials Esco reserves the right to alter its products and specifications without notice All trademarks and logotypes in this material are the property of Esco and the respective companies 9010009_Biolo Esco Global Offices Beijing China Chengdu China Kuala Lumpur Malaysia Manama Bahrain Guangzhou China Hanoi Vietnam Melaka Malaysia Mumbai India Philadelphia PA USA Salisbury UK Shanghai China Seoul Korea Delhi India Osaka Japan Manila Philippines Midrand South Africa Jakarta Indonesia Singapore escoqlobal cor eo te Ae LIVE SUPPORT S a Goceae ONE BERE
13. ciency as building exhaust fan can work less hard while achieving necessary protection e Modern separator less mini pleat filter construction maximizes the filter surface area to extend filter life and eliminate possible filter media damage by thin and sharp aluminum separators used in conventional HEPA filter construction e The filter frame and media is constructed in accordance with EN1822 requirements for fire retardant properties e The supply ULPA filter provides ISO Class 3 per ISO14644 1 clean air to the work surface in a gentle vertical laminar flow for product protection e The filter media is protected from mechanical damage by an integrated metal screen guard which is absent from conventional HEPA filters and by an angled staggered exhaust damper e The exhaust HEPA filter traps biohazardous particles acquired from the work area before air is exhausted via the ducting system to the external environment The Model LB2 is provided with an integral exhaust collar for connecting the cabinet exhaust to an airtight ducting system NOTE Ductwork is not provided with standard product Front Sash Assembly e Integrated sash proximity contacts sense sash position serve as an interlock for the UV lamp and activate an alarm if the sash is improperly positioned e The magnetic switch eliminates the chance of mechanical wear and tear typical of a mechanical switch e The sash is counterbalanced for smooth and easy
14. complete a formaldehyde decontamination process e HPV decontamination effectiveness is Independent of temperature and humidity Formaldehyde requires temperature above 20 C and relative humidity above 65 e For information on the BIOQUELL and STERIS HPV methodologies contact Esco or your Sales Representative and ask for our HPV Decontamination Whitepapers e Other decontamination methods maybe available e g Chlorine Dioxide gas All decontamination cycle parameters must be validated with appropriate methods Esco cabinets are designed to operate within a performance envelope to maintain protection for Airflow parameters used to frame the performance envelope include both inflow velocity and The graph illustrates the boundaries of the performance envelope as well as the nominal performance point at which tests are conducted The range between high and low Inflow and high and low downflow together with the fluid dynamics achieved through sophisticated cabinet design proportionally sized capture slot and uniform laminar airflow combine to deliver a complete containment and safety solution of a professional biological safety cabinet For details on the Esco performance envelope contact Esco or your Esco Sales Representative ESO WORLD CLASS WORLDWIDE 10 KI Discus Containment Test According to EN 12469 Potassium lodide Esco is currently one of the few companies in the world equipped to perform the KI Disc
15. earing life e An integral blower hour meter tracks operating life and aids in predictive maintenance planning a l a ar a Inet Filtratio Side capture zones Dynamic air barrier inflow and forward directed downflow air converge Ambient air is pulled through the front grille to prevent contamination of the work surface and work product The inflow does not mix with the clean air within the cabinet work zone Ambient air is taken in through a pre filter at the top of the cabinet and passes through the downflow ULPA filter entering the work zone as laminar flow The uniform non turbulent air stream protects against cross contamination within and throughout the work area Near the work surface the downflow air stream splits with a portion moving toward the front air grille and the remainder moving to the rear air grille A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity small blue arrows e To prevent fan damage a paper catch grille traps papers or towels that may drop down on the drain pan preventing them from being pulled into the column by fan suction Sentinel Microprocessor Control Alarm Monitoring System The Esco Sentinel microprocessor based control system supervises operation of all cabinet functions e The control panel is located on the center of the cabinet and angled down for easy access by the operator e Con
16. ersonal identification number access restriction cabinet start up protocol airflow alarm and other microprocessor controlled operations outlined in the user manual 38 When programmed ON the start up protocol shall perform an automatic pre purge and post purge cycle to ensure proper cabinet operation 39 The controller shall include a blower motor hours meter to display aggregate motor running time and UV hour meter to display aggregate UV use to assist in predictive maintenance 40 Audible and visual alarms shall be provided for unsafe conditions such as improper airflow or sash position 41 The audible alarm shall have mute feature with adjustable ringback period 42 Airflow shall be monitored by a temperature compensating thermistor based true air velocity sensor mounted in the cabinet 43 The airflow display and alarm system shall be individually calibrated before shipment 44 The main control panel shall exhibit continuous display of air velocity and a 24 hour clock display 45 Diagnostics button should be available on the control panel to easily check the cabinet operating parameters and assist servicing 46 The cabinet shall have field calibration mode that simplifies on site calibration mode 47 A selectable Quickstart mode should be available to automatically turn the blower and lights on off by moving the sash window to correct position 48 The BSC shall have RS 232 data output port for remote mo
17. for 0 9 meter 3 ft cabinet Electrical outlets are mounted below service fixtures to minimize obstructions Helpful for certifiers the hinged maintenance assembly opens to a fixed position on integrated gas spring struts providing front service access All key components with the exception of the blower motor assembly are mounted outside the air stream and away from contaminated air to permit service without decontamination These include fluorescent lamps UV lamp electrical harnesses electronic boards and microprocessor control Panels enclosing microbiologically hazardous areas are color coded to warn service technician The telescoping Dynamic Chamber plenum minimizes physical lifting and accelerates filter change when required Work area containment is maintained even when removable components are lifted out for cleaning The one piece stainless steel work tray edges are radiused and easy to clean without crevices or joints The lower drain trough is a single piece fabrication with wide open angles and a channel to direct spills to the drain The closed sidewall contains no perforations air return slots or other hidden areas where contaminants can accumulate ESO WORLD CLASS WORLDWIDE Biosafety Cabinets Standards Compliance Dynamice Chamber Plenum Design E High negative pressure I Negative pressure J Positive pressure The Esco triple wall design creates a Dynamic Chamber plenum which surrounds c
18. hall be mounted behind the control panel module out of the work zone Electronic ballasts shall be used to eliminate flicker extend lamp life and reduce heat output 28 The UV lamp shall operate via an automatic timer with automatic shut off managed by the microprocessor controller and shall be interlocked with the blower motor and fluorescent lights for safety 29 The cabinet shall be designed with a 10 angled front to optimize user comfort reduce glare and maximize reach into the work area 30 The front grille shall be raised to prevent airflow blockage and improve comfort 31 Penetrations for petcocks and service fittings shall be provided penetrations shall be offset to improve user access 32 The cabinet shall accommodate an optional mounting stand for fixed height Control and Alarm System 33 All cabinet functions shall be managed by a programmable microprocessor control system capable of software updates via Internet downloads 34 The microprocessor controller shall be mounted on the main control panel facing down toward the user 35 The controller shall include soft touch keypad controls and backlit LCD displays to permit operation of the blower motor light UV lamp electrical outlet s and menu 36 The LCD shall simultaneously display inflow downflow sash status airflow status and filter life 37 The controller shall be user programmable in situ to enable or disable functions such as PIN p
19. ic lab chair Laboratory grade construction meets Class 100 cleanliness alcohol resistant PVC materials Adjustable height 395 490 mm 15 6 19 3 Ergonomic foot rest Angled helps maintain proper posture Adjustable height Anti skid coating chemical resistant finish IV bar with hooks Stainless steel construction Available for all standard Esco cabinets Microscope viewing device Mounting and viewing pouch integrated into sash Factory installed specify when ordering Anti blowback valve Prevents blackflow of air from the facility HVAC system into the Esco product Air tight damper Seals the Esco product from the facility HVAC system during decontamination ESO WORLD CLASS WORLDWIDE General Specifications LB2 3B_ LB2 4B _ LB2 5B_ LB2 6B_ Without Base Stand With Optional Base Stand 711mm 28 type 0 9 meters 3 1 2 meters 4 1 5 meters 5 1 8 meters 6 1115 x 852 x 1610 mm 44 0 x 33 5 x 63 3 1115 x 852 x 2321 mm 44 0 x 33 5 x 91 4 1420 x 852 x 1610 mm 50 02x 33 57 05 55 1420 x 852 x 2321 mm 56 0 x 33 5 x 91 4 1725 x 852 x 1610 mm 68 0 x 33 5 x 63 3 1725 x 852 x 2321 mm 68 0 x 33 5 x 91 4 2030 x 852 x 1610 mm 380 0 x 33 5 x 63 3 2030 x 852 x 2321 mm 380 0 x 33 5 x 91 4 nto Q 2600 x 852 x 1610 mm OZ 633 55 6575 2600 x 852 x 2321 mm 102 4 x 33 5 x 91 4 970x623x715 mm Bo LX 24 Se XAB 12
20. mmable for secondary function automatic UV light All functions can be user activated through fluorescent lights and timer simplifies operation enhances contamination control extends UV lamp life and saves energy touch pad programming access see User Manual electrical outlet and orange for caution UV lamp ON Diagnostics button to easily check the cabinet operating parameters and assist Servicing E PIEPLI F Pe a o g h 4 WET a Blower hour meter and UV hour meter to track use for predictive maintenance planning ESCO Sentinel GoLto MICROPROCESSOR OOJ Sentinel Gold Microprocessor Control System Programmable When programmed ON the start up sequence confirms status with Air Safe and local time display e the Personal Identification Number PIN access restricts unauthorized adjustments e an airflow alarm warns of deviations from normal velocities e A recessed central area and stainless steel drain pan channel spills Cabinet Construction Robust construction and enhanced safety features qualify the cabinet for the most e demanding laboratory applications The cabinet is fully assembled and ready to install and operate when shipped Consult your Esco Operating Manual or contact your Sales Representative for information on user preference programming capabilities built into the Sentinel microprocessor platform The drain pan is flush with the side walls to eliminate c
21. nitoring of cabinet operating parameters 49 TCP IP converter shall be available as an option to connect RS 232 to network for remote monitoring 50 Voltage free contacts shall be available to trigger external alarm and switch ON OFF the external blower damper 51 Pressure switch shall be used to trigger the cabinet exhaust alarm and switch the internal blower ON OFF Certification Service and Decontamination 52 The cabinet shall be approved for both hydrogen peroxide vapor HPV and formaldehyde decontamination protocol 53 All panels leading to potentially contaminated and or hazardous areas shall be color coded red 54 All components with the exception of blower motor and ULPA filters shall be located outside of contaminated air spaces to facilitate servicing without the need to decontaminate the cabinet 55 All exterior surfaces shall be painted with a permanent antimicrobial inhibitor coating to minimize contamination Installation for Labculture Class II Type B2 Total Exhaust Biological Safety Cabinet Air Tight Damper Required Enables static pressure to be adjusted as filter loads to maintain airflow volume w Enables the cabinet to be sealed off during decontamination damper by Esco Check List 1 2 Ensure a Class Il Type B2 cabinet is required for the application Provide an exhaust system ductwork and blower capable of delivering the required airflow volume
22. oncealed or hard to clean spaces e The field calibration mode makes it easier for certifiers to calibrate the airflow display e RS 232 data output port enables e There are no screws in the front or sides to trap contaminants or complicate cleaning e The interior work area is formed from a single piece of stainless steel with large radius 25 mm 71 remote monitoring of cabinet operating parameters corners to simplify cleaning Tray handle to allow easy removal to Optional service fittings are offset and located at sidewall for easier access e Optional TCP IP converter to connect RS The cabinet work zone has no welded External plumbing is concealed behind 232 to network for remote monitoring joints to collect contaminants or rust dress panels to maintain aesthetics or e All stainless steel work surfaces are E RS 239 ni xternal surfaces are coated with Esco WS 292 SERIA removable for cleaning nes INTeecanc AL lsocide antimicrobial coating to protect SSFACE PORT against surface contamination and inhibit bacterial growth Isocide eliminates 99 9 of surface bacteria within 24 hours of exposure provide easy access and encourgae cleaning and surface decontamination Robust Cabinet Construction and Enhanced Safety Features Service fixtures are offset and at sidewall for easier reach Standard cabinets include two fixture provisions on each sidewall one provision on each sidewall
23. ontaminated areas with negative pressure preventing the possibility of contamination from leaks in filter seal gasket or cabinet structure The third wall conceals utilities Comfortable Ergonomic Design The LB2 cabinet is engineered for comfort utility value and safety NSF ANSI 49 USA LB2 4B2 LB2 6B2 SFDA YY 0569 China Air Quality ISO 14644 1 Class 3 Worldwide JIS B9920 Class 3 Japan JIS BS5295 Class 3 Japan e The frameless sash eliminates operator s line of sight blockage e A generous sash opening allows for easier access into the work zone provides ample room for transferring of small equipment See Specifications e The sliding window can be fully opened Max sash opening is 457 mm 18 to insert and remove larger instrumentation and equipment Electrical Safety and Certification All components meet or exceed applicable safety requirements e Each cabinet is individually factory tested for electrical safety e Documentation specific to each cabinet serial number is maintained on file e UL Listed for USA and Canada e Certified to the US standard NSF ANSI 49 for Class Il biohazard cabinetry refer to Standard Compliance table at the top Filtration EN 1822 H14 amp H13 Europe IEST RP CC001 USA IEST RP CC007 USA IEST RP CC034 USA Electrical Safety UL 61010 1 USA CAN CSA C22 2 No 61010 1 EN 61010 1 Europe IEC 61010 1 Worldwide
24. p supply This minimum exhaust static pressure for clean exhaust filter should not be used for exhaust fan sizing and it is listed here for comparative purpose only Noise reading at open field condition anechoic chamber Additional voltages may be available contact Esco for ordering information Cabinet only excludes optional stand 5 04 m 178 0 cu ft Comprehensive Performance Testing At Esco Labculture validated with the following test methods Inflow downflow velocity PAO aerosol challenge for filter integrity Airflow pattern visualization Electrical safety to EC61010 1 Additional KI Discus containment and microbiological testing are performed on statistical sampling basis Every Labculture LB2 model manufactured by Esco is individually tested documented by serial number and Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets Microbiological Testing Esco performs testing in accordance with more than 10 of the world s most recognized standards for local regional and international criteria Testing in our microbiology laboratory is conducted according to NSF ANSI 49 EN 12469 and JIS K3800 An NSF accredited biosafety cabinet field certifier is available in house full time to supervise all tests Harmless Bacillus Subtilis bacteria is used to challenge the cabinet then incubated for 48 hours and the Colony Forming Uni
25. rmrest is raised above the accessories work surface height workzone to improve comfort and to Note Updated warranty policy is available When lowered permits movement ensure that the operator s arms do not from Esco s website and the policy is subject through standard doorway block the forward airflow perforations to change from time to time Otherwise Note Increases exterior dimensions e The optional adjustable support stand Contact your local Sales Representative for Requires flexible height duct connection provides work surface height control warranty details ISSZiSE Antimicrobial Powder Coating E 2 E All exterior painted surfaces are powder coated with Esco Isocide an antimicrobial inhibitor to minimize contamination Isocide is integrated into the coating J SAES 2 substrate and cannot wash out or diminish by repeated a cleaning Performance results are available upon request is g U ca 0 4 hours 8 hours 12 hours 16 hours 20 hours 24 hours Contact Time in Hours Labculture Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets Model LB2 Biological Safety Cabinet Technical Specifications 295 mm 11 6 LB2 3 I 406 mm 15 9 LB2 4 2 643 mm 25 3 LB2 5 3 820 mm 32 2 LB2 6 1300 mm 51 2 LB2 8 4 5 T 6 EEA fle H NT 7 l 8 9 f 10 11 12 22 5 O
26. sash moving operation e The counterbalance locking mechanism is inherently safe it locks the counterbalance and sash window in place if either of the 2 cables is detached The sash cable and cable clip Esco cabinets use supply ULPA filters per IEST RP CC001 instead of conventional HEPA filters commonly found in biological safety cabinets While HEPA filters offer 99 99 typical efficiency at 0 3 micron level ULPA filters 0 15 0 20 0 25 Particle Size um 0 30 provide 99 999 typical efficiency for particle sizes of 0 1 to 0 3 micron level ESO WORLD CLASS WORLDWIDE 0 40 0 50 C Unfiltered potentially contaminated air Room air Inflow air have rated strength of more than 6x the weight of the sash window of the largest Labculture Class Il cabinet 1 8 meter 6 ft model Blower Efficiency The Labculture blower system is designed for high performance operation maximum energy efficiency and minimal maintenance e The blower motor can maintain airflow velocity within 10 of original setpoint when the pressure loss across the filter is 150 of the value of clean filters without manual speed control adjustment e The permanently lubricated direct drive external rotor motor blower reduces operating costs e The external rotor motor design allows for optimum cooling of the motor during extended operations and extends the motor b
27. t Kit provision 17 Esco Sentinel Gold microprocessor control system 18 Safety glass sliding sash window 19 Single piece stainless steel back wall and side walls 20 Removable side dress panel for plumbing access 21 Pre filter 22 Cabinet power inlet 23 Auxiliary power inlet 24 Zero voltage relay contact 25 Frequency selectorJapan cabinet only 26 RS 232 port 315 mm 12 4 623 mm 24 5 815 mm 32 0 oa 852 mm 33 5 Accessories and Options Cont Electrical Outlets and Utility Fixtures Electrical outlet ground fault North America Electrical outlet Europe Worldwide Petcock air gas vacuum North America American style Europe Worldwide style DIN 12898 DIN 12919 DIN 3537 Cabinet Accessories Germicidal UV lamp Controlled by automatic UV lamp timer through Sentinel micro processor control panel Emission of 253 7 nanometers for most efficient decontamination Lamp is positioned away from operator line of sight for safety and proper exposure to interior surfaces Note UV lamp intensity reduces over time and its effectiveness is subject to factors such as relative humidity in the cabinet ambient air temperature and microbial species in the work zone PVC armrest Chemically treated improves operator comfort easy to clean 711 mm 28 standard size Ergonom
28. t profile to achieve best downflow uniformity Frameless shatterproof sash is easier to clean Ergonomically angled front improves reach and comfort Removable one piece work surface simplifies cleaning Raised airflow grille maintains safety by preventing blockage R T A T Esco ISS lt Li DE antimicrobial coating on all painted surfaces minimizes contamination Negative pressure plenum surrounds supply positive pressure plenum no fabric bags are used The cabinets are Kl Discus tested on sampling basis for performance integrity SFDA We Available in 0 9 1 2 1 5 1 8 and 2 4 meter models 3 4 5 6 and 8 Shown with optional telescoping stand Esco Labculture has passed more performance tests in more languages for more certifications throughout more countries than any other biological safety cabinet in the world Labculture Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets race Operator Product and Environmental Protection The Esco Labculture Class II Type B2 Total Exhaust Biological Safety Cabinet provides operator product and environmental protection This cabinet can be used for handling organisms in all Risk Groups In the event of work with Risk Group 4 organisms the operator needs to Wear positive pressure suit when this Cabinet is used Containment and Protection The total exhaust design of the LB2 makes the cabinet suitable for
29. tinuous monitoring of cabinet airflow is displayed on a bright easy to read LCD panel e A temperature independent fast response pressure switch is used to trigger the airflow alarm and turn off the internal blower should exhaust become insufficient e An integrated temperature compen sated true airflow velocity sensor provides an accurate airflow reading despite room temperature fluctuation Esco Centrifugal Fan with External Rotor Motor left vs Conventional Fan with Standard Motor right Labculture Esco cabinets use German made ebm papst permanently lubricated centrifugal motor blowers with external rotor designs ntegrated blades narrow the profile and eliminate the need for a motor shaft Motors are selected for energy efficiency compact design and flat profile The completely integrated assembly optimizes motor cooling All rotating parts are unitized and balanced for smooth quiet vibration free operation A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone and prevents work surface emissions from escaping the work zone The downflow combined with the inflow air enters the common air plenum All air in the common plenum is HEPA filtered and exhausted via a dedicated ducting system to the external environment All electronic parts are contained inside a plug and play module Microprocessor soft
30. ts CFU are counted to determine the testing results Increased microbiological challenge tests with objects inside the cabinet work zone bunsen burner external airflow disturbance and Human As Mannequin test adapted from Fume Hood development were performed to simulate real world conditions Personnel Protection Test The test objective is to evaluate the safety of the cabinet for the personnel operating on potentially hazardous samples in the cabinet workzone e A nebulizer containing 55 mL of 5 to 8 x 108 spores mL B Subtilis spores is placed inside the workzone 10 cm 4 inches behind the front opening sash e Target slit air samplers and impingers are placed outside the workzone to capture B Subtilis spores that may escape then the sample is incubated e Acceptance The number of B Subtilis CFU recovered from the agar plates shall not exceed 10 CFU per test The Performance Envelope Concept High intikaw j High Downing 0 43 Hig Tiai i055 Low Downie i0 E Homainal Inflow 0 53 Homunal Downllow 031 E Lemay Wnt Hri Lowe bin They 045i iow Dewan tkew 10 26 0 25 O30 0 49 E t O45 Lanai velai Product Protection Test The test objective is to determine cabinet protection to the product samples inside the cabinet workzone from environmental contaminants e A nebulizer containing 55 mL of 5 to 8 x 10 spores mL B Subtilis is placed at 10 cm 4 inches in front of sash window e
31. us test for our customers The KI Discus test is defined in the European Standard for microbiological safety cabinets EN 12469 as a test method for validating the operator personnel protection capabilities of the cabinet e The KI Discus test shows excellent correlation with the microbiological test method for operator protection and is useful for validating the actual containment performance of the cabinet on site e The Kl Discus takes only 45 minutes as opposed to 2 days for microbiological testing e Thus Esco Labculture LB2 models are factory tested on a sampling basis using the KI Discus method for operator safety Purchase Specifications LB2 Series Class Il Type B2 Total Exhaust Biological Safety Cabinet General Performance and Certifications 1 The biological safety cabinet shall comply with one or more of the following international standards and the manufacturer shall provide a certified copy of containment and performance tests equivalent to or greater than specified in the following independent international standards for biological safety electrical and other functional characteristics Class Il Type B2 per NSF ANSI 49 USA and SFDA YY 0569 2 The cabinet shall protect a the operator and laboratory environment from particulates generated within the work zone b the product and process within the work zone from airborne contamination from ambient air c and the product and process within the
32. ware updates are available from Esco for download Sentinel functions are factory set to default to ON or OFF depending on worldwide destination and local preferences Default settings can be user activated through the touchpad data entry access The airflow alarm can be activated or deactivated depending on user preference and nature of the work Automatic start up sequence will prepare the cabinet for normal operation and advise when safe conditions are established Selectable Quickstart mode to automatically turn on off the cabinet blower and lights by simply moving the sash window to the correct position and lights on off when the sash window reaches safe sash height An administrator controlled PIN Personal Identification Number can be set to restrict access to main menu Zero Volt Relay Contacts to trigger external alarm and synchronize building exhaust blower with internal blower Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets A graphical interface indicates cabinet performance SET button doubles as Alarm mute button with adjustable Color coded indicator lamps display green for primary function Touchpad data entry buttons permit control settings and Digital read out with alpha numeric display access to diagnostics default indicates all input status and alarm functions settings and hierarchical menus ringback period fan operation blue Progra
33. work zone from cross contamination 3 Each cabinet shall be listed by Underwriters Laboratories UL cUL or CE for electrical safety 4 Original documentation specific to each cabinet serial number shall be provided with the cabinet and maintained in the manufacturers records Test data verifying all perfor mance criteria shall be available upon request to include a inflow velocity through direct inflow measurement method b downflow velocity and uniformity c filter leak scan with aerosol challenge for both filters d electrical safety 5 The cabinet shall be tested by KI Discus test European Standard EN12469 2000 on statistical sampling basis to validate operator personnel protection The retention efficiency for the front aperture shall be not less than 99 999 Microbiological testing for cabinet performance shall also be performed on a statistical sampling basis Filtration System 6 The cabinet shall have one supply downflow filter and one exhaust filter Supply filter shall be ULPA type per IEST RP CC001 and meet EN1822 H14 requirements Exhaust filter shall be HEPA type per IEST RP CC001 and meet EN 1822 H13 requirements 7 The filters shall be within an aluminum frame with mini pleat design without aluminum separators no wood or fiberboard shall be used in the filter assembly Labculture Biological Safety Cabinets Class Il Type B2 Total Exhaust Biological Safety Cabinets 8 Typical ULP
34. xhaust system designed by qualitied personnel Inappropriate design e g improper manifolds inadequate exhaust may cause Issues including but not limited to unnecessary alarm and or negate containment designed into the cabinet Inadequate exhaust and or inappropriate exhaust system design will cause the control to turn off internal cabinet s blower as one of the primary safety feature to protect user from inadequate containment Integrated Filtration System A combination of a supply ULPA filter and an exhaust HEPA filter give the Labculture cabinet a fully integrated performance envelope for product operator and environmental protection ULPA supply filter per IEST RP CC001 is tested to a typical efficiency of gt 99 999 for 0 1 to 0 3 micron particles creating cleaner work zone than conventional cabinets Mini pleat Separatorless Filter left vs Conventional Aluminum Separator Filter right Esco cabinets use Camfil Farr mini pleat filters without aluminum separators to increase filter efficiency minimize the chance of leakage and to prolong filter life Filters include a lightweight aluminum frame for structural stability and elimination of swelling common to conventional wood frames Esco ULPA Filter Efficiency Typical Penetration 0 0002 2 N e HEPA exhaust filter per IEST RP CC001 is tested to a typical efficiency of gt 99 99 for 0 3 micron particles achieving better energy effi
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