SX-8G IP-Star® Compact
Contents
1. Reagent Tip Rack 2 for 1 5 2 0 5 0ml tube It holds 8 Tips for single pipetting 4 Screw Capped Tubes of 1 9ml or 2 0ml and 4 Screw Capped Tubes of 9 0ml Reagent Reservoir Rack It holds up to 8 Medium Reagent Container or up to 4 Large Reagent Container Container L is double sized of Container M It receives discarded Tips coming down through Waste Chute Reagent Tip Rack 1 for 1 5 2 0ml tube It holds 12 Tips for single pipetting and 12 Screw Capped Tubes of 1 amlor 2 0ml Waste Chute It leads discarded Tops to Waste Tip Box Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 15 Stylus Pen Stylus Pen Holder It is used to operate instrument It is a holder for Stylus Pen and can through Touch Panel It could also be magnetically attached onto the be used as a ballpoint pen instrument surface Disposables Disposables delivered by Diagenode Cat No Product Description Place to be Used WA 006 0010 Medium Reagent Container for SX 8G Compact Reagent Reservoir Rack WA 007 0020 Large Reagent Container for SX 8G Compact Reagent Reservoir Rack WC 001 1000 Tips bulk 96 Tip Rack Reagent Tip Rack 1 2 WC 02. 6 Remove Y Axis fixing jig to right side base by removing 3 fixing screws Then replace Right Side Cover and fix it by two screws 7 Open Left Side Cover by removing 2 fixing screws 8 Remove Y Axis fixing jig to left side base by removing 4 fixing screws Then replace Left Side Cover and fix it by two SCrews 9 Connect Power Cable to Power Inlet Connector on the right side of the instrument Then turn Power Switch on assuring that Front Door and Waste Tip Box Door are closed securely IN Do not use power cable other than provided to avoid issues such as temporary power failure or risk of fire 10 Installation completed securely and that cushioning Is set between Magtration Unit and Stage Unit to protect nozzles from free movement When transferring the instrument make sure that all the fixing jigs for all axes are attached Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 14 DIAGENODE SX 8G COMPACT USER MANUAL Accessories and Disposables Accessories Description Quantity 96 Tip Rack Reagent Reservoir Rack Reagent Tip Rack 1 Reagent Tip Rack 2 Waste Tip Box Waste Chute Stylus Pen Stylus Pen Holder Power Cable for AC240V Power Cable for AC110V O ring Silicon Grease Fuse 96 Tip Rack It holds Tips up to 8x12 pieces
3. 200008 Axis Endlimit error its movable range Protocol run shall be restarted from Error other than errors described 200001 Axis error the beginning after replacing all above l disposables needed Note e The errors listed above are usually caused by inadequate setting of disposables accessories or if the door is not shut properly Be sure to check the placement of accessories disposables and the door before restarting protocol run e f there are error codes other than the codes listed above or the error could not be eliminated by performing the countermeasure described contact Diagenode with recording the error codes Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com Ordering information Description Cat No Format IP Star Compact B03000002 1 unit Visit us at one of Diagenode s demo sites or discover our Automated Systems by performing some assays with the help of our R amp D and Technical Department www diagenode com diagengte Innovating Epigenetic Solutions Diagenode s a BELGIUM EUROPE LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Belgium Tel 32 4 364 20 30 Fax soy 30A VA 25 orders diagenode com infoOdiagenode com Diagenode Inc USA NORTH AMERICA 400 Morris Avenue Suite 101 Denville NJ 07834 USA Tel 1 862 209 4680 Fax 1 862 209 4681 orders na diagenode com info na diagenode c
4. After the user presses the next button in the Layout information screen the Set confirmation screen appears At this point user is expected to be ready to press RUN Buttons e The user presses the Back button Returns the user to the display of the screen they came from e The user presses the Run button This is the expected action when user gets to this display after reviewing blocks Runs the protocol Running status Protocol name ooo Progress Bar pa am bd Remaining time 03 05 47 Left block C Right block e ma Current Temperature Value diagencdue Screen Running After the user presses the Run button in the Set confirmation screen the Running screen appears Buttons e The user presses the Stop button Then screen shall be changed to Stop Dialog Status screen is preferred as a progress bar that moves across the screen as the step progresses Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 26 DIAGENODE SX 8G COMPACT USER MANUAL Running status diagengtie Stop Dialog Finish e Open the door e Recover the samples and remove the consumables 100000000 2300000000 2 diagengde Stop Dialog Buttons e The user presses the Stop button Then screen will be changed to Finish or End and stop the protocol e The user presses the Back but
5. If you want to continue the protocol run from halted point after adding disposables you will be responsible for the result When Front Door is opened after halting operation power to the motors Is shut by safeguard resulting displacement of stopping positions of motors Liquid leakage from Tip end O rings are not greased enough or Grease O rings properly or replace O rings or big difference in liquid level O rings are deteriorated It is recommended that replacement of O rings is made by among lanes an appropriately trained Field Service Personnel to avoid damage of the nozzles If you replace O rings by yourself you will be responsible for the result Disposables are not set properly Check for proper setting of Mictotiter Plates PCR Plates etc Note that using disposables other than recommended may cause instrument malfunction It is strongly recommended to use those disposables designated for the protocol Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 31 List of Error Codes Code No Message Description Countermeasure to be taken 200007 Axis Z bottom sensor Z Bottom Sensor was activated Check the position of the module corresponding to the error reported and remove the obstruction caused the error Then home all axes Axis other than Z was moved beyond
6. It leads waste tips and liquid to Waste Tip Box Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 12 DIAGENODE SX 8G COMPACT USER MANUAL Example of loading racks and disposables Heat Cool Units 96 Tip Rack Mi al YAA i k y val T 0 Microplates Reagent Tip Rack 1 e 96 Tip Rack Holds 8x12 pieces of DN7O Tips e Reagent Reservoir rack Holds Reagent Container M or L e Reagent Tip Rack 1 Holds 12 Tips for single pipetting and 12 Screw Capped Tubes of 1 5ml or 2 0ml e Reagent Tip Rack 2 Holds 8 Tips for single pipetting 4 Screw Capped Tubes of 1 5 ml or 2 0 ml and 4 Screw Capped Tubes of 9 0 ml e Microtiter Plates Holds 96 Wells Microplates e Waste Chute Place where waste tips etc are discarded Note The above layout is an example and it could vary depending the protocols Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 13 Installation Long screw 1 Remove protection films attached on the instrument 2 Open Front Door and fix Grip of Front Door by two screws supplied 3 Remove the fixing jig for X Axis Magtration Unit fixed by 3 screws inside instrument 4 Remove cushioning and desiccant agents 9 Open Right Side Cover by removing 2 fixing screws
7. Compact by avoiding Places exposed to direct sunshine Places with vibration high humidity or dust Places where strong electric or magnetic fields exist Places where liguid or oil could be splashed to the machine Places where flammable gas corrosive gas or high heat source exist Damage to or malfunctions of the instrument may be caused by inadequate placement of the system Storage e Unplug power cable from wall outlet when the instrument may not be used for extended period of time Precautions for Instrument Operation e Possibility of biohazard from samples or extracted purified products could not completely be eliminated in operating the instrument Use gloves in operating the Instrument for your safety according to the description in this book e Pay close attention in handling samples with high risk of biohazard e When operating the instrument under low environmental temperature keep the instrument powered on e Be sure no foreign materials get into the instrument from outside e Do not scratch or press the operation panel using knives or materials with sharp edges e Replacement of the fuse shall be made by field service personnel authorized by Diagenode Contact us for fuse replacement About Warranty Period e 12 months after installation regardless of its usage e This warranty excludes problems derived from inadequate storage inadequate usage or operation repair modification maintenance of the instrument b
8. is the first active window it allows the user to enter into Ol three different parts of the software USER ACTIONS Protocols Maintenance Information Buttons diagengue e Protocols e Maintenance e Information Protocols Protocols screen y Se Y A y All available protocols are displayed on this screen Sample prep hMeDIP MethylCap 2 E ae a LO e lt Re ChIP MagBisulfite d Library prep diagencde Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 22 DIAGENODE SX 8G COMPACT USER MANUAL Information screen DIAGENODE HEADQUARTERS Diagenode sa BELGIUM EUROPE Diagenode Inc USA NORTH AMERICA After the user presses the Information button the Information screen Avenue de hospital 1 400 Morris Avenue Suite 101 Tour GIGA 3rd Floor Denville NJ 07834 USA 4000 Liege Belgium Tel 1 862 209 4680 appears Tel 32 4364 20 50 Fax 1 862 209 4681 Fax 324 364 20 51 orders na diagenode com orders diagenode com Info na diagenode com info diagenode com Buttons e The user presses the Back button to return to the display of the Top menu screen they came from diagencde Screen Categories Name Protocol List After the user presses the Categories Namel button the Categories Name appears When selected the protocol on the protocol list the Run button shall turn exec
9. protocol run select STOP button And Protocol Select screen is displayed The aborted protocol can not be restarted anymore Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 30 DIAGENODE SX 8G COMPACT USER MANUAL Troubleshooting guide Presumable Cause Countermeasures To Be Taken Power is not supplied to AC Power Cable is not connected Check connection of AC Power Cable Be sure to use instrument properly Power Cable supplied with instrument Do not use power cable other than supplied Fuse is burned out Contact Diagenode if fuse needs to be replaced Displayed characters are not Light intensity is low Adjust light intensity properly by rotating the screw located readable on the right of Display with flat blade screwdriver Error code is displayed Causes vary by error code Record the error code displayed and take appropriate countermeasure referring to List of Error Codes If the countermeasure taken does not eliminate the problem contact Diagenode for assistance Protocol was started with Be sure to confirm appropriate setting of reagents and incomplete setting of Tips or disposables before starting protocol run other disposable If you want to add disposables after protocol started press STOP to abort instrument operation It is strongly recommended to restart the protocol run from the beginning after completion of adding disposables
10. the work surface layout background The magnified view provides a better display of the correct method setup for that block on the work surface Sample number 8 Layi it infarmatinn Reagent information E Buffer A 6200 0ul Reagent information A Magnetic beads 190 0ul B Buffer C 1350 0ul C DIB 900 0ul D Ab in buffer A 900 0ul Block Regent Buffer rit infarmatinn Block Regent Tip Rack MTP 182000200080 2000008080 om AAAAAA G 0 2000008 E oARAARADRDARANAA 00000008 Sample number 8 PCR tube E Sample 8 AYYY 0 O O000088 lt 9 000008 o OO00008 1 O000008 f aoaouvuiordrk 8 7 6 5 4 3 2 1 Block MTP Block PCR Tube Save Parameter Confirmation Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 25 Set confimation Tu Select a Protocol name Protcol and Sample EA ChIP 8 DIB 100 ptd Protocol OI Input value in the Sample Number Sample number a x io ati a i l Configuration Input value in the Ab coating 1 0 h Middle Configuration IP reaction 1 0 h Washing 2 0 min Current temperature Left block C Right block C E o a current Temperature Value diagengte Screen Set confirmation
11. 02 0960 Tips box 96 Tip Rack Reagent Tip Rack 1 2 WA 003 0010 96 well microplates Microplates Module WA 001 0080 200 ul tube strips 12 tubes strip cap strips Heat Cool Units aawa a a a desc Unis WA 008 0100 2 0 ml screew capped tube Reagent Tip Rack 1 2 Medium Reagent Container Large Reagent Container Tips Note Disposables needed vary by application and also disposables other than listed above may be recommended in some applications Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 16 DIAGENODE SX 8G COMPACT USER MANUAL Reagents Refer to each Application Manual for reagents to be used and directions for use e Do not reuse any disposables reagents to avoid contamination or instrument malfunction e Do not use disposables reagents other than specified to avoid instrument malfunction e When discarding disposables follow the instructions defined by your facility or regional laws Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 17 Basic hardware operation Front Door Operation Front Door moves up and down and it is held at the uppermost position when fully moved up Hold only the Grip of the Front Door to open or close the door to avoid fingers getting pinched Front Door is locked d
12. DIAGENODE SX 8G COMPACT USER MANUAL Storage Conditions o o sssini ksantana 5 40 C Relative Humidity 19 75 Non Condensing Barometric Pressure mamiscceiiciostiai n More than 70kPal min Transport Conditions a A A 25 60 C Relative HUMIGILY 0 19 75 Non Condensing Barometric Pressure cccccccececeeeeeeeeees More than 70kPal min Environmental Requirements A 15 30C Relative Humidity 19 75 Non Condensing A A Up to 2 000 m SE AE O E E E T Keep distances from walls as shown below for ventilation maintenance work etc Also keep more than 200mm space over the instrument More than 200 mm SX 8G Compact DIAGENODE SX 8G COMPACT USER MANUAL PAGE 9 Place the instrument on horizontal surface Do not place the instrument where it will get direct sunlight or vibration When operating instrument in a cold chamber or cold room keep the instrument power on to avoid condensation About Caution Labels Inside Instrument Caution Label for High Temperature The part where this label is attached becomes hot Be sure not to touch while working Caution Label for Biohazard Place attached Waste Chute The part where this label is attached inside cover has the possibility of biohazard contamination with samples or extracted purified products Take care to avoid contamination Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PA
13. Field Service personnel e Stage and Racks should be cleaned after every run e Nozzles and Piercing Unit of Magtration Unit should be cleaned after every run Cleaning Procedure e Stage e Magtration Unit e All Racks e Waste Chute e Waste Tip Box In addition to the list above the inside of the instrument and the accessories should be cleaned after each use Cleaning Method Choose cleaning liquid depending on the contamination status and wipe the contaminated surface using paper such as Kim Wipe moistened with the cleaning liquid Use a mask goggles and gloves when cleaning the instrument for your Safety e Water e 70 Ethanol Note Do not use alcohol when cleaning Touch Panel or Clear Panel of Front Door Use water for those parts Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 28 DIAGENODE SX 8G COMPACT USER MANUAL Greasing O rings Grease O rings every two weeks to maintain proper tip attachuent and avoid liquid leakage e A 1 Remove old grease 2 Wear gloves and put small amount of Silicon Grease on the fingertip Then spread Silicon Grease on fingertips by rubbing fingertips together 3 Put Silicon Grease on O rings black rubber rings on Nozzles 4 Place a tip on the nozzle to spread the grease evenly around the O ring Be sure not to put an excess of Silicon Grease as it may result insufficient tip discard Europe D
14. GE 10 DIAGENODE SX 8G COMPACT USER MANUAL System Components Front View Waste Tip Box Access Door Right Side View On the right side of the instrument The Power Switch and Communication Port Connector are located at the front and Power Inlet Connector and Fuse Unit are located at the back ii Communication Port Connector RS 232C for maintenance Note Replacement of the fuse shall be made by field service personnel authorized by Diagenode Contact us for fuse replacement Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 11 Internal Components The Magtration Unit and Stage Unit are located inside the instrument Magtration Unit _Heat Coot Unit Rack Holder Waste Chute 1 Magtration Unit e Nozzle Unit This unit has 8 nozzles to process up to 8 samples simultaneously with accurate and rapid aspiration dispensing e Magnet Eight magnets are aligned with 8 tips to simultaneously separate magnetic particles for up to 8 samples 2 Stage e Heat Cool Unit Two Heat Cool Units are equipped and can hold 8 and 12 tubes stripes Temperature can be set for 4 to 95 C depending on protocols e Rack Holder Racks and Disposables to be used vary by protocols Refer to Application Manual for details e Waste Chute
15. UAL Basic Software Operation for running applications Start Up and Shut Down Procedure Start Up e Close access door for Waste Tip Box and Front Door Refer to sections 7 2 Putting On and Taking Out Waste Tip Box and 7 1 Front Door Operation e Turn on Power Switch located on the right side of the instrument e When the instrument is turned on the Touch Panel screen is activated Shut Down e Turn instrument power off making sure that the instrument is not operating If it is operating stop operation by pressing Protocol Stop button Touch Panel Operation Using Stylus Pen select desired function e Operate Touch Panel with Stylus Pen gently without excessive force to avoid damage e Use only the Stylus Pen provided Do not to damage the Touch Panel screen by using tools with sharp edges Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 21 Running a protocol Diagenode Splash Screen A0 Automated Epigenetic Applications After the software start up screen disappears the Diagenode splash OP screen is displayed for several seconds and then disappears Magtration Technology Genetein software ver 1 0 Copyright 2010 diagencde Start Screen Top menu After the Digenode splash screen disappears the start screen Is displayed This
16. diagengde Innovating Epigenetic Solutions IP Star Compact Fully Automated System for Epigenetic Applications J y l A r USER MANUAL Cat No B03000002 Version 1 08 2016 Guarantee Limited one year global warranty Diagenode guarantees all products from any manufacturing defects as we rigorously test all products to meet strict quality standards Diagenode warrants that all standard components of its instruments will be free of defects In materials and workmanship for a period of one 1 year from the date that the warranty period begins unless the original quotation or accompanying documentation states a different warranty period All warranty periods begin on the date of delivery and apply only to the first purchaser of the product If a manufacturing defect arises and a valid claim is received within the warranty period Diagenode at Its discretion will repair or replace the product in accordance with the warranty terms and conditions stated herein In case of repair or replacement of a product under warranty Diagenode will cover the expenses to return the repaired or replacement product This warranty covers only manufacturing defects and does not cover any damage caused by misuse lack of compliance to recommendations stated in the manual neglect accidents abrasion or exposure to extreme temperatures chemical solvents or acids We strongly recommend that maintenance o
17. dure scr ia eaa ho R ERE RG DRE DERE HE MRE ED RRS OO 20 quer Panel UNEN eres ques Parade apa RULE ee HE De A Shure ais HES PR ee 20 PUBIC a POCO semed iei sodio da esta dde ee Boo aes E odiosa ins da dea da Ts de be a 21 Weir Se ee ee eee ee ee ee ee ee ee ee ee ee ee di ee ee eee 27 Cleanind PrOCeQUre pii cee ace eben Gece es Pees phe d oh be Se de Sew ea este eer ee she deed thes gee a Era IN ONUSMNO S oicaGae en crees edil tta aed E ends as uti 28 o PP ctnemeeaeeee ie etneemane seers 29 List OP Error COUCS sas ain aes pen eee eee dae ee eee eer R EA 31 Ordering NI OFM OM a cati n e ie ot oa dee ee ead whe ds oe Oe Ree eh Re ee oa a Back Cover Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 4 DIAGENODE SX 8G COMPACT USER MANUAL User Information Thank you for your purchase of the Diagenode System SX 8G IP Star Compact which has been developed for the automation of different epigenetic applications This book is a guide for the user to operate the instrument safety and effectively the instrument Please read this book thoroughly to understand the instrument before starting operation Also keep this book nearby the instrument a quick reference for all the operators as needed Do not operate the instrument without sufficiently understanding instructions described in this book Always keep this book nearby for easy reference at any time Below is a list of the differ
18. e ooo omens oe Raw ace Rew me em eee 2 Caution Label tor High Temperature 2 ee benew es bed fu ee ete eee eG ek new ee a bhedaw es 7 Caution LAD STOP DIOS ZAG e wie hed bo adn Ok Hae bee oe wees ood wth a System COMPONCINUS icc uo eya eee teenie eon amet nae oie nad eae nee pee ica ea na 10 ELN MS a repost aia bee ee Hb eG See Sa E ratio ica Seed ee Vere 10 PG oide Me ss ramiro a eee eared He ai BEd PRR G Soe eee es oh eu Bote eee Os 10 tera SCION GIN SS cay xs ees ea one ee oe EE Gee dee hoes a Bra Ree e EE Poe oP 11 Sta UAH 45 in PUE e eee dane eee eee en own see ee Goren eee ee Ge ee ee ee tee 13 Accessories and DISpOSaDleS erence nde a ence aa Kod a aaa he eee eae od aoe 14 o o AAA 14 Beee PIES abono persons rondar opa e aa ono error oo a vea 15 Disposables delivered by Diagenode 00 ccc eee 15 REIS artesa erecta cease o arroba eas hee od aoe a e tea hate a paa zen 16 Basic for Hardware OpPerauon oesie s rra A A RRA AO ACA RARA EER EES 17 Pront Door Uber IOs gunn enira ney sae eee baw oak Se ir te rn ee een oe eo ee ea 17 Putting Unand Taking Uut Waste Tip BOX 2c0c oceeceee seo lisas cera ax ee coy Sees ares aby 17 petting Racks and ISposables I Place wads tereina nieee baw ees bey alee deeedyadeeel ees obey 18 Setting POR Plate onto Heat Cool Unit odd cexg esd eee hawa sae yia geai dag outs beg ces ode denen 19 Basic Software Operation for Running ApplicationS aaan eee ees 20 start Up ano Shut Down Proce
19. ell microplates Set Microtiter Plates in positions designated by the selected application taking care of positioning direction am a p i AR ty r A a a a AA ta kt E 4 mM AAA 444 4 X A a m LANA AA AAA AAKAD gt al E a h 1455455 iki a A B C When 96 well microplates are not positioned correctly adequate aspiration dispensing or liquid mixing may not be performed resulting inadequate extraction purification If non recommended microtiter plates are used adequate aspiration dispensing or liquid mixing may not be performed resulting inadequate extraction purification Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 19 Setting PCR Plate onto Heat Cool Unit Place PCR Plate on Heat Cool Unit taking care of the positioning direction and push it down to fit completely When PCR Plate is not positioned correctly adequate aspiration dispensing or liquid mixing may not be performed resulting inadequate extraction purification If non recommended PCR plate is used adequate aspiration dispensing or liquid mixing may not be performed resulting inadequate extraction purification Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 20 DIAGENODE SX 8G COMPACT USER MAN
20. ent devices included in the Instrument Package No Description Quantity Remarks 1 SX 86 Compact Main Body 1 2 Grip of Front Door 1 w two fixing screws 3 96 Tip Rack 4 4 Reagent Reservoir Rack 1 5 Reagent Tip Rack 1 1 for 1 5 2 0ml tubes 6 Reagent Tip Rack 2 1 7 Waste Tip Box 1 8 Waste Chute 1 9 Stylus Pen 1 10 Stylus Pen Holder 1 11 AC240V Power Cable 1 12 AC110V Power Cable 1 fem O ring 8 14 Silicon Grease 1 For O rings foe Fuse 1 16 Users Manual J E 1 ea This book 17 Package Check List 1 Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 5 Safety Information Before using the SX 8G IP Star Compact please read the safety instructions carefully Please read this manual thoroughly to understand and to get familiar with preventive maintenance procedures to avoid any problems in instrument operation Be sure that no water or liquid would get into the instrument or that the instrument does not get wet It would cause fire or electric shock Do not attempt disassembling modifying or repairing by yourself lt may cause malfunction of instrument or electric shock to you Ask us for repair or maintenance of the instrument Installation e Choose an adequate place for installing the SX 8G IP Star
21. iagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 29 Troubleshooting Error Reported During Protocol Run Error Error code reported is shown in place of xxxxx in the above screen And Error Code XXXXXX the corresponding error message is displayed under the code Axis Z bottom sensor The protocol run aborted by an error can not be restarted from the point of abortion e Refer to the List of Error Codes for error codes e The screen shown above is displayed and the instrument aborts its operation when an error occurred during a protocol run e Take appropriate countermeasures for the error referring to List of Error Codes attached e Make note of the error code reported because it is requested when contacting Diagenode for technical support Note Repeat the same countermeasure when the same error occurred in the next run of the protocol Contact Diagenode when countermeasure taken does not resolve the problem Suspending or Aborting Protocol Run e When you want to suspend a protocol run operation select Suspend button in the display to suspend instrument operation It may take some time before suspending its operation depending on the process under execution e Select START button to restart and continue the protocol run e When you want to abort the
22. n Returns the user to the display of the Protocol List screen they came from O om m e The user presses the Next button Then screen shall be diagenode E ae changed to Layout information Ab corting Mixing time e The user presses the Save Parameter button Then screen will be changed to Save Parameter Confirmation OK Current parameters shown in the Display View will be stored to the Protocol ptd And returns the user to the display of the Configuration screen No Returns the user to the display of the Configuration screen e The user presses the Text box Then screen will be changed to Keyboard or Speed list menu Keyboard Confirmation Middle Fast Overwrite parameters Middle Low Speed list menu Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 24 DIAGENODE SX 8G COMPACT USER MANUAL Layout information E Screen Layout Information After the user presses the next button from Sample number screen or Configuration screen the Layout Information screen appears Buttons e The user presses the Back button Returns the user to the display of the screen they came from e The user presses the Next button Then screen shall be changed to Set confirmation i infarmatinn Tip Rack DN70 e When the user presses a block that block is magnified on
23. om For a complete listing of Diagenode s international distributors visit http www diagenode com pages distributors html For rest of the world please contact Diagenode s a
24. r repairs of Diagenode s products are performed by our approved Diagenode service center Improper or incorrectly performed maintenance or repairs will void the warranty Technical Assistance amp Ordering Information Diagenode s a BELGIUM EUROPE Diagenode Inc USA NORTH AMERICA HEGE SCIENCE PARK 400 Morris Avenue Suite 101 Rue Bois Saint Jean 3 Denville NJ 07834 USA 4102 Seraing Belgium Tel 1 862 209 4680 Tel 32 4 364 20 50 Fax 1 862 209 4681 Fax 32 4 364 20 51 techsupport na diagenode com techsupport diagenode com orders naUMdiagenode com ordersUdiagenode com For a complete listing of Diagenode s international distributors visit http www diagenode com en support distributors php For the rest of the world please contact Diagenode s a DIAGENODE SX 8G COMPACT USER MANUAL PAGE 3 Contents Soci i a a a E ee ee ee ee ee ee ee ee 4 Safety Informa lOM darts tae cede Saad Sevens Me Reba tee eae Meee eee ade cta aii o aida ade dl 5 SPCCINCANONS 64rcuneiursienee suey laa tdci tah eee eee etek ye ee ee ese pend eee ene 7 FeatUreS naau uaaa 7 POT Cc ma opa ia a pida EE ee ee ee ee ee ee ee ee ere 7 Size Weight and Power Requirements 0 000 eee ete 7 rad ONG a raras esas esas puja Ree da neem eee een ork ene dy a meee ue Reena ad 8 Tonene Cond ae a eer ee eee eee eo ee eee ee ree ee ee TO ere ee eee ee ee ee 8 Environmental Requirements 8 About Caution Labels Inside Instrument Sowa pa oo e
25. ton Returns the user to the display of the screen they came from Screen Finish End When the protocol is complete a window appears telling user the run is over The screen behind this window should be the Startup screen When OK is pressed then the Startup screen appears and the user can immediately begin to remove their sample and prepare for the next run At this point user is expected to be ready to press RUN Buttons e The user presses the OK button Then screen shall be changed to Categories Name Protocol List Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 27 Maintenance Item Frequency Done by Cleaning Stage and Racks After every run User Cleaning Magtration Unit After every run User Greasing O rings Every two weeks User Replacing O rings Every 6 months Field Service Maintenance Once a year Field Service Use a mask goggles and gloves while maintaining the Instrument for your safety according to the description in this book Maintenance work shall be performed with instrument powered off Pay close attention when cleaning places where caution labels are attached e Maintenance work shall be done as Indicated otherwise the results of the protocol runs may not be accurate e Certain maintenance will be performed by Diagenode
26. uring operation Open Front Door after it completely finished its operation Waste Chute Waste Chute can be removed by taking it out from Waste Tip Discard Hole for cleaning Waste Tip Box Waste Tip Box can be removed for discarding Tips or for cleaning Open Front Door first and then open access door for Waste Tip box Take out Waste Tip Box by holding Its grip Make sure that the access door for Waste Tip Box Is closed after setting Waste Tip Box otherwise you cannot start a protocol run IN Gently open and close the access door to avoid damage when removing the waste Tip Box Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 18 DIAGENODE SX 8G COMPACT USER MANUAL Setting Racks and Disposables in Place Setting Racks Set Racks required by the selected application at each designated position taking note of positioning direction Be sure to place Racks Inside frames completely without uplift 96 Tip Rack Reagent Reservoir Rack Reagent Tip Rack 1 Reagent Tip Rack 2 No positioning Position the cut to Spring Tubes on front side Tubes on front side When Racks are not correctly positioned Tip pickup failure inaccurate aspiration dispensing or instrument malfunction may occur When Reagent Tip Racks lor 2 are not correctly positioned Tips will not be picked up resulting instrument malfunction F f E dl e de a Setting 96 w
27. utable Buttons e The user presses the Back button Returns the user to the y display of the Protocols screen they came from Bagencus e The user presses the Shutdown button Then screen shall be changed to Power Off e The user presses the Run button Then screen shall be changed to Sample number e A Page up the list box V Page down the list box Sample number Screen Sample number After the user presses the Run button the Sample number appears Sample number Buttons e The user presses the Sample number Text box Then screen will be changed to keyboard e The user presses the Back button Returns the user to the display of the Protocol List screen they came from diagenotie e The user presses the Next button Then screen shall be PE tic ici number changed to Configuration or Layout information Keyboard Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 23 Configuration C Screen Configuration Mixing time Temperature Mix Speed After the user presses the next button from the Sample number screen Ab coating LOh 40 Middle the Configuration screen appears IP reaction 1 0h 40 C Middle Buttons Washing 5 0 mi Middle O e The user presses the Back butto
28. y dedicated Graphical User Interface e Compact with minimal installation footprint e Multiple procedures available for various type of magnetic particle reactions by using special protocol development software e Simultaneous processing of up to 8 samples by 8 nozzles Performance Instrument Oasise rnaak Desktop aa Samples 1 8 samples batch Processing time About 30 min 1 8 samples Temperature Control ssec Two Heat Cool Blocks 4 95 C Handling VOLUMIG cidcid inten 9 200 ul Dispensing CCUTACY ccccccccccccccccnnnnannnnnns 9 10 pl less than CV 20 11 20 ul less than CV 10 21 200 pl less than CV 2 Accuracy with distilled water at room temperature 20 25 C Note Processing Time depends on protocols Size Weight and Power Requirement A EN A W800xD700xH675mm disregarding prongs WV CUNY isso 97kg excluding accessories and disposables Power Required coooooococcccccccnnncnnninicininnnos Power Required AC100 240V 50 60Hz 440VA e Do not share power source with other instruments e Do not place close to instruments that emit noise or can cause a power fluctuation e Connect power cable to wall outlet with ground line Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 8 Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com
29. y people who are not authorized by Diagenode though within warranty period Diagenode Inc North America Phone 1 862 209 4680 Fax 1 862 209 4681 Mail orders na diagenode com PAGE 6 DIAGENODE SX 8G COMPACT USER MANUAL About Disposals e Contact us when you want to disposal the instrument Warning In case of an emergency e fan emergency situation of the instrument occurs such as extraordinary heat smoking abnormal smell etc immediately unplug the power cable from wall outlet to avoid fire or electric shock to you Please contact us for technical support e If foreign material or water is splashed inside the instrument immediately unplug the power cable from wall outlet to avoid fire or electric shock Please contact us for technical support e f power cable becomes damaged core wire exposed or broken immediately unplug the power cable from wall outlet to avoid fire or electric Please contact us for technical support Europe Diagenode sa LIEGE SCIENCE PARK Rue Bois Saint Jean 3 4102 Seraing Ougr e Belgium Phone 32 4 364 20 50 Mail info diagenode com DIAGENODE SX 8G COMPACT USER MANUAL PAGE 7 Specifications SX 8G IP Star Compact instrument employs MAGTRATION technology to perform multiple protocols for different epigenetic applications i e ChIP MeDIP and MethylCap Features e Full automation of epigenetic applications using Magtration technology e Simple operation b
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