User`s Manual - INHECO Industrial Heating & Cooling GmbH
Contents
1. Target Temp Validation Tool Accuracy C 20 C 68 F 1 37 C 98 6 F 1 60 140 F 1 5 80 C 176 F 2 Uniformity measured in well Uniformity is defined by the maximum difference between lowest and highest temperature according to a Greiner 96 Flat Bottom A1 A12 E7 H12 Target Temp Validation Tool Uniformity C 37 C 98 6 F lt 08 Evaporation in unsealed Micro Plates Incubator temperature 37 C 98 6 F max 10 loss of liquid in 4 h deionized water room temperature 24 26 C 75 2 78 8 F rel humidity 40 50 altitude at 410 m over sea level Heatup time Start temperature 15 6 C 60 F Target temperature 37 C 98 6 F Heat up time max 30min Volume 280ul per well The target temperature is reached when all sensors are in the tolerance band of 37 C 1 C 98 6 F 3 6 F Measured with 1 Greiner Flat Bottom Plate 655076 with 4 sensors A1 H1 E7 H12 BA IncubatorMP 24 5 3 Module Dimensions Weight Xmodule Picture 17 Dimensions 5 4 1 Dimensions and Weight Parameter Value Dimension in 149 0 mm 1 268 5 mm without connector cables Dimension in Ywodue 2 298 5 mm with connector cables Dimension in without pin 52 0 mm Incubator Open drawer incl door Y Drawer 193 5 mm Dimension of thermal chamber in Zchamber 23 5 mm Weight without cabl2. Incubator with serial number TTxy Picture 14 Interconnection BA_IncubatorMP 20 The starting basis is as follows USB drivers installed on PC The PC is connected to the bottom Incubator of a stack via USB The INHECO tool is installed on the PC All COM ports are closed All Incubators are daisy chained power is switched on and they are connected to the PC After pushing the button Find Incubators COM Ports all COM Ports will be scanned and some commands of the Firmware Command Set will be automatically executed to determine the configuration The results will be written into the textbox of the tool and the combo boxes for COM port and device ID will be set respectively In more detail Find out all available COM ports Open next available COM port If none go to 18 Send an arbitrary command for synchronisation and announcement If no reply gt Close COM port and go to 7 Else important Look if a device ID error is present and look at the reply this is working since firmware version CC 3 17 gt Device ID of that stack master device Send RDAO 1 to the master on this COM Port with the device ID found gt Number of floors Slave devices Send RFV2 to the master and all its slave devices take care of the floor ID 00 gt T01 gt T02 up to the number of slave devices Store serial numbers COM port name number of devices and device ID Close
3. 85 F Do not use materials that are not sufficiently temperature resistant up to 110 C 230 F Explosive flammable and volatile materials may not be heated in the Incubator at the risk of explosions 3 Operating Instructions 3 1 Abstract INHECO s compact single position Incubators are high performance plug amp play devices for the use on automated robotic platforms or as standalone units They can either be used as single devices or stacked in a tower In addition multiple towers or single devices can be controlled parallel from one host PC via USB The combination of different devices of the Incubator family in one tower is possible Picture 1 Stacked Devices The Incubators have built in safety features which enable them to be used in IVD and FDA applications Technical Specification chapter 5 The Temperature can be reported during processing to have a maximum of control Precise temperature and shaker control USB interface plate loading sensors and several self test routines guarantee a safe precise and fast processing of probes The Incubator is a state of the art device for incubating SBS listed micro plates including flat bottom and PCR with adapter plate Service support is guaranteed through INHECO BA IncubatorMP 10 3 2 Conventional Usage The Incubator is a state of the art device and complies with today s standards The manufacturer attached much importance to the user s safety The following rules a
4. D 2 but only one slave is connected to the master 15 Timeout sub device The error message is from this device The sub devices did not answer within the expected time frame The Incubator has two uCs The heater controller work as a kind of sub devices of the communication controller If the inter uC communication is corrupted this error code will be reported BA IncubatorMP 2T Most error codes can be used for a PC lab automation application to generate its own error codes or to change automatically its operation status E G if the device ID error is reported the device ID must be changed Ox60 End of reply 10 Typical process example A typical cycle for a process should contain the following steps y v Vv V V V V V V V V V VV V Scan COM Ports Find device ID slaves and serial number of all connected devices Check the number of drawer cycles and the firmware versions of all devices Initialize all devices Open doors simultaneous or sequential Putin the Labware and the adapter if necessary Set target temperature for each Incubator Set heater offset according to the used type of micro plate Close the doors Start heating Check cyclic temperatures and the error flags Optional change target temperature Optional restart heating Stop heating Open all doors 11 Warranty 2 Years from date of shipment Any damage by abuse or caused by operation different from this instruction is not covered BA_Inc
5. IFICATIONS TECHNICAL DATA THERMAL FUNCTIONALITY MODULE DIMENSIONS WEIGHT 5 4 1 DIMENSIONS AND WEIGHT 5 4 2 DRAWER SPECIFICATIONS 6 FINAL QS TESTS BA_IncubatorMP 10 10 11 11 11 12 13 13 14 14 15 19 19 22 23 23 24 25 25 26 26 6 1 6 2 6 3 10 11 12 13 DRAWER TEST FIRMWARE AND HARDWARE STRESS TEST PERFORMANCE TEST CALIBRATION AND ADJUSTMENT AUTOMATICALLY SELF TEST AQS DETAILED DESCRIPTION OF THE ERROR CODES TYPICAL PROCESS EXAMPLE WARRANTY POWER SUPPLIES DECLARATION OF CONFORMITY BA_IncubatorMP 26 26 26 26 26 27 28 28 29 32 1 Explanation of Symbols and Abbreviations A possible danger leading to serious bodily harm is being pointed out to you Caution hot surface A possible danger leading to less serious bodily harm is being pointed out to you This signal also warns you of tangible damage A A possibly dangerous situation leading to material damage is being pointed out to you Important This sign refers to useful information as to installation etc Bullet points refer to enumeration gt These arrows indicate instructions The squares refer to procedures running automatically and the results to be achieved BA_IncubatorMP 7 1 1 Abbreviations The document uses the following terms CC Communication controller Controller Microprocessor with on chip peripheral CRC cyclic redundancy Check FCS Firmware Command Set Heater
6. Same as Temperature Controller IVD D In vitro diagnostic directive MP Micro Plate PCR Polymerase Chain Reaction PWM Pulse Width Modulation Shaker Controller for shaker regulation TBC To Be Continued Temperature Controller Controller for heat regulation USB Universal Serial Bus CE Conformity mark NRTL Nationally Recognized Testing Laboratory DLL Dynamic Link Library PC Personal Computer EU European Union SBS Society for Biomolecular Screening DWP Deepwell Plate IVD In vitro diagnostics FDA Food and Drug Administration DIP Dual in line package BA_IncubatorMP 2 Safety Instructions Avoid accidents Always make sure that the unit is disconnected from the power supply system during any installation process BA_IncubatorMP Electric Shock Hazard You can suffer an electric shock if the unit is not connected properly or if you did not disconnect the unit from the power supply system before opening the housing Please observe the following measures in order to avoid muscle convulsions burns unconsciousness apnea or even death Do not work with open housing when unit is connected to the power supply system Always switch off the unit before implementing any alterations The unit is operated at a voltage of up to AC 240V Burn Hazard You can burn your skin when touching the inner parts and especially the microplate contact surface and disposables These parts can reach up to 85 C 1
7. ckable INHECO devices Liability does not apply for mishandling the unit This manual is part of the Incubator and must be e retained until the Incubator is disposed of e passed on to the new user when the Incubator is sold or lent Please contact the manufacturer in case you do not understand something within this manual Your opinion about this manual provides us with valuable insights on how we can serve you better Please do not hesitate to direct your comments to us using the address or the phone numbers on page 3 Please read the safety instructions very carefully They must be understood and observed in order to ensure safe handling of the unit Missing or insufficient knowledge of the manual leads to loss of liability against INHECO The operator should therefore ask for an instruction confirmation from the manufacturer to ensure proper handling of the device BA_IncubatorMP 4 CONTENT 1 1 1 2 3 3 1 3 2 3 3 34 3 5 3 6 3 7 4 4 1 4 2 4 3 4 4 4 5 5 5 1 5 2 5 3 EXPLANATION OF SYMBOLS AND ABBREVIATIONS ABBREVIATIONS SAFETY INSTRUCTIONS OPERATING INSTRUCTIONS ABSTRACT CONVENTIONAL USAGE WHO IS PERMITTED TO OPERATE THIS UNIT SERVICING THE INCUBATOR TECHNICAL ALTERATIONS MALFUNCTIONS NAME PLATES AND LABELS OPERATING THE INCUBATOR SCOPE OF SUPPLY INITIAL OPERATION USB COMMUNICATION COMMUNICATION OF INCUBATORS TO THE USB HOST PC COMMANDS TECHNICAL SPEC
8. e device on an appropriate plate for a firm and even stand Use the position pins on the bottom side for exact positioning of the device stack Important The plate device has to be in balance for proper performance Picture 3 Details The devices are built for the use with microplates The Labware detection is optimized for Greiner Flat Bottom Plates If different microplates are used the user has to assure that the Labware detection works well with these plates gt transparent micro plates are better than dark ones gt microplates with matt surface are better than shiny ones S gt Attaching an adapted tape on the left side of the microplate improves the success rate of the Labware detection Important The bottom of the microplates must be flat to ensure uniform heat exchange If the customer prefers plates with different shaped bottom a special adapter is needed INHECO offers to design and manufacture a customized adapter BA_IncubatorMP 16 The devices can measure the temperature at the top of the heat plate only The temperature of the liquid in the microplate will differ from that Therefore a heater offset must be defined and set to adjust the temperature and to get the desired temperature in the liquid samples see also command SHO After Cmd AID for Initialization is send to the Incubator the door will close if door was open If the door was already closed a short buzzing sound can be heard The heat plate has thre
9. e integrated sensors for measuring the temperature The temperature of the sensors is averaged Electrical Details General The device needs an electrical power supply that provides a stable 24Vdc voltage The power consumption for one device is max 1 25 Ampere 30 Watt The polarisation at the power connector at the back side is shown in the following picture The red wire symbols the plus pole and the black the minus pole To communicate with the device a computer with an USB Connection is necessary The USB Connector is placed at the back side see blue mark at picture 4 O000000 o 55555555 O O connection Master Device O Picture 4 Standard connection for one device BA_IncubatorMP 17 Address Switch A six pole DIP Switch can be found at the back side Only the switches 1 to 4 are used for setting the address These switches are for identification if more than one single device or stack is connected to the PC The default address is 2 The recognition of the device stack depends on the switches position as shown in picture 5 PERT HADES ti JS adel Jel Le 9 6 1 HADES REIR Picture 5 Address Switch Connecting Devices B B g lo E B B B B EL e A maximum of 6 Incubator devices can be operated with one power sup
10. es 2 5 kg Incubator BA_IncubatorMP 25 542 Drawer Specifications Parameter Value Accuracy of the end position of the drawer in x y and z drawer open single unit all parts at 0 30 mm specified ambient temperature Maximum Drawer load 0 25 kg Open drawer resistance for a force in Z axis 10 without plastic deformation 6 Final QS Tests All units which leave INHECO have to pass through three semi automatic test procedures 6 1 Drawer Test The device must pass at least 100 drawer cycles with correct Labware detection without error 6 2 Firmware and Hardware Stress Test To ensure reliable behaviour of the devices the firmware especially the USB communication is extensively tested for 10 minutes The test is done that way that the hardware is tested parallel to the communication test 6 3 Performance Test Finally all important functions are tested and the self test AQS see chapter 8 must run without error 7 Calibration and Adjustment The calibration of the temperature is done at INHECO Settings are stored into the devices EEPROMS and redundant in an INHECO Database A redundant check of all data is done 8 Automatically self test AQS The automatically self test AQS is performing several tests to ensure proper functionality of the device After the tests are finished the device generates an error code see Firmware Command Set These tests are gt Drawer Test Checks
11. es is similar to the configuration of stacking 3 devices picture 7 4 3 USB Communication Hard and software features of the Incubator USB virtual COM port are specified in this section Remark The USB is not optimized for secure real time data transfer Therefore all communication is secured by a cyclic redundancy checksum crc If the communication between the PC and the MTC does often fail or results in timeouts in most cases the PC is causing the problem Therefore we recommend for a robust communication the following 1 Other devices connected to the USB might have an influence on the communication stability of the incubator Use a simple workstation PC The stability of the communication has to be verified with every PC Windows 7 and Vista seems to be more stable than Windows XP Do not activate the automated update from Windows ARON 4 4 Communication of Incubators to the USB Host PC The example has the following configuration gt Five devices daisy chained with the D Sub D connectors Two power supplies are needed one for two and one for three devices Two devices in one stack One single device A second single device VV v BA_IncubatorMP 19 The LED indicates the status of the module e Power on and device is initialised gt gt LED on e Firmware download gt gt LED is blinking ca 3 5 Hz e USB Communication not present gt gt LED is blinking ca 1 Hz Details shown in the picture below
12. inheco INHECO stackable devices Incubator MP Part No 7300003 Document Nr 900236 001 User s Manual Revision level V1 5 March 2012 BA IncubatorMP 1 INHECO Industrial Heating and Cooling reserves the right to modify their products for quality improvement Please note that such modifications may not be documented in this manual This manual and the information herein have been assembled with due diligence INHECO does not assume liability for any misprints or cases of damage resulting from misprints in this manual If there are any uncertainties please feel free to contact us The brand and product names within this manual are registered trademarks and belong to the respective titleholders BA_IncubatorMP 2 How to contact us INHECO 82152 Martinsried Germany Telephone Sales Telephone Technical Hotline Fax E Mail Sales E mail Technical Hotline Website This manual belongs to Type Serial No Year of Manufacturing Order Confirming No To be filled in by customer Inventory No Place of installation BA IncubatorMP 089 899 593 101 089 899 593 201 089 899 593 499 sales inheco com techhotline inheco com www inheco com PRODUCT SERVICE Important Notes Read this manual carefully before using INHECO stackable devices In the following the names of the stackable devices Incubator Shaker or Incubator are used They represent one device of the family of sta
13. is not mentioned in it his paragraph to prevent damage to the Incubator MP e eo BA IncubatorMP 11 Decontamination D The most common decontamination method is by fumigation with formaldehyde or ethylene oxide gas The surface decontamination can include a wipe down of the housing surface A solution of 70 alcohol should be used where effective for target organisms The use of Bleach 5 12 or Microside SQ is also possible Repair The Incubator may only be repaired by authorized INHECO service technicians either locally or at INHECO s Shut down and Disposal D The unit is to be disposed in accordance with the environmental directives in effect in the respective country Caution ce The Incubator may only be operated in an upright and level position Important The Incubator unit must not be stored below 10 3 5 Technical Alterations e Do not alter the product For safety reasons no technical changes to this unit are allowed by unauthorized persons Any modification or change which is not amp approved by the manufacturer in written form leads to loss of warranty e The original parts are especially designed for the Incubator Parts provided by other suppliers are not tested and therefore not approved by INHECO Using such parts can impair the functionality of the unit and will lead to loss of warranty e For damages which may occur due to the usage of non original parts or non approved modifications or repair
14. ist for this device This flag is set if the command is completely unknown E G if a shaker command has been sent to an Incubator without shaker function 3 Invalid operand Parameter out of range see command set This error code will be reported if the Parameter of a correct command is out of range E G STT1234 4 Protocol error USB Protocol error Wrong message length or CRC The USB Message is secured by a cyclic redundancy check at the last byte of a message If the last byte is incorrect or missing this error code will be reported 5 Timeout from device Device did not answer within the expected time frame This error must be generated by the PC application 6 Device ID error The device ID can be changed at the dip switch on the rear side of the device If the message one sends to this device has the wrong device ID this error is replied 7 Device not initialized Command requires prior initialization of a sub system E g It is not possible to open the drawer of a non initialised device 8 Command not executable Parameter or device condition prohibits execution 9 Drawer not in end position The drawer is in an unknown position generated by RDS E G the door is blocked 10 Unexpected Labware Status A Labware is detected on AOD after a power up or after an undefined drawer Status 11 Reserved 12 Reserved 13 Reserved 14 Floor ID error Device is not registered in the master E G A command has been send to the slave device with I
15. opening and closing of the drawer gt Temperature Test Tests timing and homogeneity of the heating procedure including boost gt Duration 3 min 30 sec BA_IncubatorMP 26 9 Detailed description of the Error Codes The error codes embedded into the message reply are mainly meant to give the user additional information about the status of the device The reply to every command ends always with the following three hex data 0xB0 device ID This ensures that it is a reply from the device with this device ID 0x20 error code The following table 1 describes the error codes see also Firmware Command Set Table 1 error codes Code Error message Detailed description 0 Message Ok Normal return message 1 Reset detected Power on software or watchdog reset Three kinds of resets are possible i Power on reset gt after the first ordinary message reply after power on this error code will be send This is a correct behaviour ii Software reset gt after the command SRS the green led in front of the device blinks shortly The software reset brings the device into the same state after power on Therefore also here this error code will be send iii Watchdog reset gt if the firmware runs into trouble in most imaginable situations a watchdog reset occurs This error code will be send Please inform the INHECO Tech hotline in such a case 2 Invalid command The Command does not ex
16. ply and controlled via one USB interface the 15 pole SUB D Connectors on the back side are used to daisy chain the devices The following pictures show how the devices have to be connected Slave Device addressing 654321 USB O slave connection O Master Device 654321 USB O 3 addressi Picture 6 two connected devices BA IncubatorMP Two Devices The Master Device will be connected to power and USB The DIP Switches can be used for identification The COM Cable 1 connects the two devices These lines support the Slave Device communication and power The Power and USB Connector and the DIP Switches of the Slave Device are unused and have to be left in the standard configuration as shown 18 654321 USB O Three Devices The Master Device will be connected to power and USB The DIP Switches can be used for identification o The COM Cables green 1 and blue 2 connect the three s5a321 usp devices These lines support the Slave Devices communication and power The Power and USB Connector Slave Device I o and the DIP Switches of the Slave Devices are unused and have to be left in the standard configuration as shown Slave Device E b 654321 USB O TEES Picture 7 three connected Devices connection Master Device The configuration for stacking more than 3 devic
17. pply to the user e Rules of accident prevention D e General rules for technical safety e EU and other country specific directives The conventional usage contains the usage according to the manual You must able to disconnect the main power supply to the instrument immediately if necessary 3 3 Who is permitted to operate this unit Only instructed and skilled persons are permitted to operate this unit Only specialized staff is allowed to make any amendments to the operating menu e 4 Servicing the Incubator Run the Command AQS every 100h see Chapter 8 Temperature calibration may be checked and repeated regularly Every 15000 operating hours or three years whatever occurs first an authorized INHECO service technician should be check the device Calibrate INHECO recommends calibrating the Incubator device annually Cleaning Before cleaning the Incubator MP disconnect the power and make sure that the temperature at the heating plate is below 50 The contact surface should be cleaned regularly to ensure optimum heat transfer to the disposable Always clean the contact surface if there has been a spillage Use the cloth dipped in 50 50 water isopropanol solution and make sure that no deposits are left on the surface Care should be exercised to prevent water from running inside the unit Do not use aggressive cleaning fluids such as acetone or abrasive cleaners Please check with INHECO any cleaning method that
18. s liability is excluded by INHECO Important BA_IncubatorMP 12 3 6 Malfunctions Report occurring malfunctions immediately to the responsible person named on page 3 of this manual Ensure that the unit is secured against violation and misuse Before the initial operation dismantled safety relevant parts have to be mounted and checked 3 7 Name Plates and Labels Please check all name plates and labels and ensure that their legibility is maintained at all times during the life time of this unit Replace all name plates and labels if their legibility is no longer ensured BA_IncubatorMP 13 4 Operating the Incubator 4 1 Scope of Supply Before initial operation make sure that the shipment of your unit is complete and no parts are damaged The following components should be included in each shipment 1 Incubator unit 2 USB Cable between Incubator and PC 15 pole SUB D COM Cable 4 USB stick containing Manual and Software The Power Supply and Power Cable are not part of the scope of Supply they have to be ordered separately see chapter 12 Picture 2 Scope of supply BA IncubatorMP 4 2 Initial Operation Transportation Picture 3 Packing of Incubator MP 1 Paperboard and foamed plastic 2 Packing of Incubator MP in plastic foil 3 Packing of Incubator MP in foamed plastic Only use INHECO original package for gerneral transport BA IncubatorMP 15 i Mechanical Integration Place th
19. t for electrical equipment for measurement control and laboratory use EN 61010 2 010 2004 Particular requirements for laboratory equipment for the heating of materials EN 61000 3 2 EN 61000 3 3 Electrical equipment for measurement control and laboratory use EMC requirements EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements This product is in complies with the essential requirements of the Low Voltage Directive 73 23 EWG and EMC directive 2004 108 EC when used for its intended purpose Place Date Martinsried Munich March 2012 Signature Position G nfer Tenzler Managing Director BA IncubatorMP 32
20. th Device ID 3 and Serialnumber s TT26 TT30 TT11 TT48 TT20 At Port COM4 are 1 Incubator s with Device ID 2 and Serialnumber s TTOS IncShak 3 22 04 2007IncShak C 3 22 04 2007RFV gt IncShak 3 22 04 2007 Picture 15 Application Example The COM ports may change therefore please use the device ID to have a unique identification of a stack Parallel communication to different COM ports is possible gt Parallel communication with different members of one stack is forbidden and may result in errors 4 5 Commands All commands for the integration of the single Incubator are described in the document Incubator FCS Document NR 900334 XXX BA IncubatorMP 22 5 Technical Specifications 5 1 Technical Data Input current Power 1 25A 30W max 0 i Tolerable relative humidity ambient PONG HOH BA_IncubatorMP 23 5 2 Thermal Functionality Parameter Value Temperature range Room temperature plus 5K to 80 C 176 F Accuracy absolute The accuracy is defined as the difference between the target temperature of ambient the heating plate and the average of the measured temperature on the temperature stability 1K heating plate The heating plate temperature is measured at 5 positions on the heating plate according to the INHECO measurement head PM 0105 The 5 measurement positions are according to the Plate positions A1 H1 E6 12 and H12
21. the COM port Goto 7 Write the results to the textbox and fill the combo boxes of device ID and COM port name and the tabs accordingly Finished see picture 16 for the result Now you are able to open four COM ports Just click at Open COM on the four tabs For the implementation it is important to consider different setup conditions Some devices may be already connected to the USB green LED is on some may not green LED is blinking at 1 Hz The blinking of the LED stops only after the first message with the correct device ID is sent to the Incubator device Therefore you need to send at least two messages to bring all devices into the same state The reason for that is that slave devices have to register themselves to the master device within a certain time frame This happens after the first correct USB message to the master was sent 1 If you have used for example a USB Bluetooth adapter on your PC Windows sometimes creates lots of COM ports for this purpose If you plan to use that PC to control Incubators we recommend deleting all these COM ports first BA IncubatorMP 21 IVDD Incubator Shaker Control D xl 1 COM7 228 COMS 5 COMS 11 COM Fifth Com Port Find Incubator Shakers Com Ports amis coms Floor fo zj At Port COM are 1 Incubator s with Device ID 1 and Serialnumber s TTO5 At Port COME are 2 Incubator s with Device ID 2 and Serialnumber s 27 TT28 At Port COMS are 5 Incubator s wi
22. ubatorMP 28 12 Power Supplies The Power Supplies can be ordered separately at INHECO Picture 17 Power Supply built for 1 and more incubators Power Supply built for 1 and more incubators AC Input 100 240V 2 5A 50 60 Hz DC Output 24V 7 5A Part 2400121 Picture 18 U K Power Supply Cord set Part 1004214 BA_IncubatorMP 29 Picture 19 European Power Supply Cord set Part 1004184 Picture 20 North American Power Supply Cord set Part 1004185 BA IncubatorMP 30 Table 2 Version Management ma NO RELEASE DATE pore TO PRIOR VERSION November 2008 2008 5 IER TEE NRTL logo page 13 USB stick Minor correction on page 11 15 February 2010 Abbreviations p 8 Cleaning p 11 Transportation p 14 Technical data p 30 February 2010 Technical data p 30 February 2010 Dimensions p 32 14 February 2011 Power supply Part 2400122 canceled p 36 only one power supply Part 2400121 for 1 and more incubators March 2012 Redesign of Document BA IncubatorMP 31 13 Declaration of Conformity INHECO GmbH 82152 Martinsried Germany Declares that the following product Incubator MP Meets the essential requirements of the following European Union Directive s using the relevant section s of the normalized standards and related documents shown Standard and Date of Issue IEC 61010 1 2001 229 Edition EN 61010 1 2001 274 Edition Safety requiremen
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