251061 benchpro QRC 25June10.indd
Contents
1. package and pierce the reagent tray foil seal with piercing device supplied with the instrument Press Next Maxiprep Plasmid Purification Set up 4 of 9 Place a disposable cell liner reservoir into the waste tray 4 Place the Cell Liner in the reservoir slot in the Waste Tray Press Next Intended Use Maxiprep Plasmid Purification Set up 2 of 9 Remove cap and place an empty 2 mL collection tube into the elution tube slot 2 Uncap and label the supplied elution tube 2 mL Place the labeled tube into the elution tube slot on the tray Press Next Maxiprep Plasmid Purification Set up 5 of 9 Open instrument drawer and place the waste tray reagent tray into the slot 5 Open the drawer unit and place the Waste Tray with Cell Liner and Reagent Tray into the instrument Press Next For research use only Not intended for any animal or human therapeutic or diagnostic use invitrogen ry Life technologies Maxiprep Plasmid Purification Set up 3 of 9 Place pierced reagent tray into the autoclavable waste ie tray ait 3 Open the drawer unit and remove the Waste Tray Place the Reagent Tray in the Waste Tray slot Press Next Maxiprep Plasmid Purification Set up 6 of 9 Pour 125 mL of 2 0 to 2 4 OD culture into disposable cell liner and cover with lid 6 Add up to 125 mL of overnight culture into the Cell Liner Place the lid on2. BenchPro 2100 MaxiCard Plasmid Purification Instrument Cat no MC1001 Rev Date 25 June 2010 Part no 25 1061 QUICK REFERENCE MAN0002695 CARD Instructions for using the BenchPro 2100 instrument for maxiprep plasmid preparation are described below For detailed instructions see the manual supplied with the instrument or download the manual from www invitrogen com Growing E coli Culture Use high copy plasmid size ranging from 3 kb to 20 kb Grow transformed E coli cells overnight in LB Luria Bertani medium with the appropriate antibiotic The bacterial culture should have a cell density of approximately 10 cells mL or an optical density of 2 0 2 4 at 600 nm OD 600 Starting BenchPro 2100 Use a bacterial culture in transition between exponential phase and stationary phase 1 Install the BenchPro 2100 instrument as described in the user manual Press the power switch located on the left side of the unit to turn ON the BenchPro 2100 instrument 2 After the startup screen is displayed press the Start function key to go to the setup screen Press the Setup function key to set up the instrument for 1 maxiprep purification To perform 2 maxiprep purifications perform the indicated step twice Performing Maxiprep Plasmid DNA Purification Maxiprep Plasmid Purification Set up 1 of 9 Pierce reagent tray foil with the provided piercing tool 1 Remove a Reagent Tray from the
3. d the card appropriately Do not reuse the card Note You are unable to open the drawer unit until the card is removed 2 Open the drawer unit to remove the elution tube containing purified plasmid DNA Cap the elution tube To avoid repeated freezing and thawing of DNA store the purified DNA at 4 C for immediate use or aliquot the DNA and store at 20 C for long term storage 3 Remove the used Reagent Tray and Cell Liner and discard appropriately Any waste generated during the protocol is collected in the Cell Liner and Waste Tray cavity Discard waste solution appropriately If needed you can remove the Waste Tray to wash and or autoclave To run another protocol press the Next function key to return to the initial protocol setup screen Load new Reagent Tray and Cell Liner Add cell culture and insert a new card Start the protocol as described above There is no cooling period required between runs 6 If you are not using the instrument turn OFF the power switch located on the left side of the unit Refer to the user manual for detailed troubleshooting information Cleaning and Maintenance Clean the surface of the BenchPro 2100 instrument with a damp cloth Do not use harsh detergents or solvents to clean the unit Clean the bottom of the instrument drawer with a light spray of 70 ethanol and then wipe the ethanol with a paper towel Clean the Waste Tray with diluted bleach solution 10 and or autoclave For any othe
4. r repairs and service contact Technical Support Do not perform any repairs or service on the BenchPro 2100 instrument to avoid damaging the unit Limited Use Label License This product is covered by Limited Use Label License No 5 See the manual or www invitrogen com for detailed information 2010 Life Technologies Corporation All rights reserved The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners D TM t3 l NV l t ro e e n For technical support email tech_support dinvitrogen com 2 For country specific contact information visit www invitrogen com ry Life technologies
5. the Cell Liner Press Next For technical support email tech_support dinvitrogen com For country specific contact information visit www invitrogen com BenchPro 2100 MaxiCard Plasmid Purification Instrument Maxiprep Plasmid Purification Set up 7 of 9 Maxiprep Plasmid Purification Set up 9 of 9 Maxiprep Plasmid Purification Set up 8 of 9 Please check the following before closing door Elution tube is labeled and uncapped Reagent tray is pierced Cell culture volume is below maximum fill mark Cell liner is covered with lid Close instrument drawer Insert Card s Press Start 7 Ensure that you performed Steps 8 Gently close the drawer unit of the 9 Insert a BenchPro Maxiprep Card 1 6 If you have accidentally missed instrument into a slot in the instrument such a step press Back and perform the Note Once the card is inserted you that the tips are inserted into the missed step Press Next cannot open the drawer reagents placed in the reagent tray Using the Card Alignment Guide place the card correctly in the slot until you hear a click Press Start 10 Press Run on the protocol set up screen to begin the protocol The protocol begins and various steps of the protocol are displayed on the screen The display shows Protocol Complete when the protocol is complete Unloading Procedure for the BenchPro 2100 1 At the end of the run remove the card from the slot and discar
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