ES-20-60 - User manual
Contents
1. Microbiology with heating Extraction Cell growing PST 60HL Ze PST 100HL oo lt with heating eign i PSTSOHLA PSU 10i id RTS 1 Applications ELISA analysis Hybridization Applications DNA analysis Genome sequence 7 ES 20 with heating c Applications Agglutination Extraction i Gel staining _ destaining Multi Bio 3D TS 100 with heating TS 100C with heating and cooling Applications _ _ _ _ _ _ sS Agglutination Extraction Blot hybridisation cd J Gel staining destaining Volume of liquids 103 10 ml Erlenmeyer flasks Cultivation flasks and 50 ml tubes 10 ml Petri dishes vacutainers and tubes up to 15 ml 10 107 ml PCR plates microtest plates and Eppendorf type tubes www biosan lv Biosan Sia Ratsupites 7 build 2 Riga LV 1067 Latvia Phone 371 674 261 37 Fax 371 674 281 01 E mail marketing biosan lv2. Time 4 3 Usingthe A and Y Time keys Fig 1 1 set the required working time interval in hours and minutes increment 1 min Pressing the key for more than 3 sec will increase the increment Speed set RPM 4 4 Using the A and Y RPM keys Fig 1 2 set the required shaking intensity in revolutions per minute increment 10 rpm Pressing the key for more than 3 sec will increase the increment Temperature set Temp C 4 5 Using the A and W Temp keys Fig 1 3 set the required temperature increment 0 1 C Pressing the key for more than 3 sec will increase the increment The set parameters can also be changed during operation A Caution The chamber heating can be turned off only by setting the temperature value below 25 C Page 6 Program execution 4 6 Fix the glassware with samples on the platform 4 7 Press the RPM Run Stop key Fig 1 4 The platform will start rotating and the timer indicator will start counting up the time interval with 1 min precision Note If the speed is set to zero pressing the RPM Run Stop key will start the timer but the platform will not move 4 8 After finishing the program after the set time elapses the platform motion will stop and the timer will be showing the flashing reading STOP accompanied by the repetitive sound signal until the RPM Run Stop key is pressed 4 9 If the working time is not set the timer indicator in the upper line shows 00 00 pressing the RPM Run Stop k
3. necessary Complete the table below and retain for your records Model ES 20 60 Orbital Shaker incubator Serial number Date of sale Page 10 8 Declaration of Conformity DECLARATION OF CONFORMITY Manufacturer BioSan Ltd Address Ratsupites Str 7 build 2 Riga LV 1067 Latvia Product name Shaker Incubator Type ES 20 60 We BioSan Ltd certify that the above mentioned product has been manufactured according to the regulations of the following European directives proven through complete compliance with the following standards M Directive Low Voltage Directive ELECTRICAL EQUIPMENT DESIGNED FOR USE 2006 95 EC WITHIN CERTAIN VOLTAGE LIMITS EMC Directive 2004 108 EC ELECTROMAGNETIC COMPATIBILITY No Standard EN 61010 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements Part 2 010 Particular requirements for laboratory equipment for the heating of materials EN 61326 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements Vasily K Bankovsk g UV if A G a Gos President Head of R amp D Department Biosan Ltd Riga 15 06 2009 Version 2 03 December 2013 How to choose a proper Shaker Rocker Vortex l Medical Biological Research amp Technologies Multi Bio RS 24 Cr o gt n SS Pee Applications ES 20 60
4. 1 C e Temperature Control range 0 0 eeeeeeeeeeeeeeeeeeeeeeeees 10 C above ambient 80 C Temperature Stability sccccsecnceecadssceezneeceeceennesncuesseney eae e O a a pi E aiia 0 5 C e Heat up time till max temperature inside the flasks 5 2 General specification e Speed control range ececeeeeeeeeeeeeeeeeeeeeeeteeeeees 50 250 rpm increment 10 rpm e Digital time setting e Maximum continuous Operation time eee cee ceeeee eee eeeeeeeeeeeteeeeenaeeeeenees 30 days Recommended interval between operation sessions not less than 8 hours Maximum lOad cccik erin Reena Rik bn ia Alene Hale 8 kg ONDIG E A nea tain hia pA a ahead 20 mm DISplay tae ge cece ve ce fees hoa be af aalg pauses dens e eas nas leafed gaddvet ens osea 2x16 signs LCD DIMENSIONS Arira eea aeae e e aee a aidai Ena 590x525x510 mm e Dimensions of the inner chamber sssssesereeerieerreererens 460x350x400 mm e Operating voltage power consumption 230 V 50 60 Hz 450 W 2A or 120 V 50 60 Hz 450 W 4 5A TATSIT O ares beste EEE AEE EE ss cachuaad bad A EA A 41 1 kg Accurate within 10 Optional Description Catalogue number accessories Platform with clamps for 30x100 ml flasks P 30 100 working dimensions 360 x 400 mm BS 010135 BK 2 Platform with clamps for 16x250 ml flasks 7 16 290 working dimensions 360 x 400 mm BS 010135 CK Platform with clamps for 9x500 ml flasks P 9 500 wor
5. D Medical Biological Research amp Technologies ES 20 60 Orbital Shaker incubator Operating Manual for version Certificate V 2AD V 2AE Contents Safety Precautions General Information Getting Started Operation Specifications Maintenance Warranty and Claims CoN Oa AOUN Declaration of Conformity Page 2 1 Safety precautions The following symbols mean A Caution Make sure you have fully read and understood the present Manual before using the equipment Please pay special attention to sections marked by this symbol GENERAL SAFETY Use only as specified in the operating manual provided The unit should be saved from shocks and falling The unit must be stored and transported only in a vertical position see marking on the package After transportation or storage keep the unit under room temperature for 2 3hrs before connecting it to the electric circuit Use only cleaning and decontamination methods recommended by the manufacturer Do not make modifications in design of the unit ELECTRICAL SAFETY Connect only to electric circuit with voltage corresponding to that on the serial number label Do not plug the unit into an ungrounded power socket and do not use an ungrounded extension lead Ensure that the power switch and plug are easily accessible during use Disconnect the unit from the electric circuit before moving Disconnect the pl
6. ey will start continuous operationof the unit with countdown timer in the lower line and indication OFF in the upper line until the RPM Run Stop key is pressed again A Caution At the end of the set time period the platform movement is stopped automatically but the heating can be stopped ONLY manually by reducing the temperature using the W Temp key Fig 1 3 lower key till the OFF sign appears in the upper line of the display 4 10 The timer can be restarted during the unit operation if necessary Press the Timer Run Stop key once Fig 1 5 to stop the timer Press the Timer Run Stop key again to restart the timer 4 11 The platform motion can be stopped at any time by pressing the RPM Run Stop key In this case the program realisation and the platform motion will stop and the timer will switch into the STOP mode saving previously set time Press the RPM Run Stop key to repeat the operation with the same time and speed 4 12 At the end of operation set the Power switch in position O Off Disconnect the power cord from electric circuit Page 7 5 Specifications The unit is designed for operation in cold rooms incubators and closed laboratory rooms at ambient temperature from 4 C to 40 C in non condensing atmosphere and maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C 5 1 Temperature specification e Temperature setting range 25 C 80 C increment 0
7. king dimensions 360 x 400 mm BS 010135 AK Platform with clamps for 6x1000 ml flasks P 6 1000 working dimensions 360 x 400 mm BS 010135 DK Flat platform with non slip silicone mat PP 400 working dimensions 360 x 400 mm BS 010135 FK Biosan is committed to a continuous programme of improvement and reserves the right to alter design and specifications of the equipment without additional notice Page 8 6 Maintenance 6 1 6 2 6 3 6 4 If the unit requires maintenance disconnect the unit from the electric circuit and contact Biosan or your local Biosan representative All maintenance and repair operations must be performed only by qualified and specially trained personnel Care and Cleaning Standard ethanol 75 or other cleaning agents recommended for cleaning and decontamination of the stainless steel surfaces The door s window is made of of organic glass poly methyl methacrylate Plexiglass and is prone to scuffing and scratches if improperly cleaned Care during cleaning of the door s window is recommended to reduce wear of the Plexiglass surface Caution Clean the door s window with a mild detergent never use organic based compounds pure alcohol alcohol containing cleaners more than 15 or ammonia containing cleaners for Plexiglass Do not use abrasive pads or cleansers For decontamination it is recommended to use a special DNA RNA removing solution e g DNA Exitus PlusTM RNase E
8. on request P 6 1000 platformi ksiecia a otters on request PP 400 platform Osanna n an anand on request 3 3 Setup place the unit upon even horizontal non flammable surface at least 30 cm away from any flammable materials Note Ensure that the unit is placed on solid level surface which is able to support it s weight remove protective film from the display plug the power cord into the socket on the rear and position the unit so that there is easy access to the power switch and plug 3 4 Platform installation remove the silicone mat from the platform secure the platform on the stands on top of the unit with the four screws using the provided hex driver and cover the platform with the silicone mat Page 5 4 Operation SET PARAMETERS 37 0 37 0 Temp C 00 25 STOP Time hr min 160 000 RPM 3 Time RPM Temp Timer Fig 1 Control panel 4 1 Connect the unit to a grounded power socket Set the Power switch on the front side to position ON 4 2 The display will turn on with the upper line showing the previously set time speed and temperature and the lower line showing current readings of the same parameters chamber temperature C which automatically starts rising according to the temperature setin the upper line Setting the parameters Use the readings in the upper line of the display while setting the parameters required Setting time
9. t brushless fan provides precise temperature distribution inside the chamber from 10 C above ambient up to 80 C The inner chamber is made of stainless steel State of the art motor newest thermal insulation materials used program provided soft start of the platform motion and temperature regulation PID control decrease the energy consumption and make the shaker incubator highly energy efficient despite its relatively large size Page 4 3 Getting started 3 1 Unpacking Remove packing materials carefully and retain them for future shipment or storage of the unit Examine the unit carefully for any damage incurred during transit The warranty does not cover in transit damage A Caution Due to its size and weight 41 kg the unit requires two people to lift or move it 3 2 Complete set Package contents Standard set ES 20 60 Orbital Shaker InCubator eee eee eect eeeeeeeeenneeeeeneeeeneeeeeeaa 1 piece POWEN CONG E A E A 1 piece spare fuse inside fuse holder eee eects eeeeeeeeeeeeeeneeeseaeeesneeeeeeaeeeeeas 1 piece four screws and a Wrench 2i cch avait dean N E E 1set Operating Manual Certificate siasii asioi i 1 copy Optional accessories P 30 100 platform Dasairas taaa on request P21O 250 platfom D sscccc 2 seed sscees sececedscsnezhieds tune peaedbeceedane teeceed tevez se einerd on request P 9 500 platform sceiseitesstsce eecceeeneecines san esnan cbtedeciceessteenncbetnaeennes estate
10. ug from power socket to turn off the unit If liquid penetrates into the unit disconnect it from the electric circuit and have it checked by a repair and maintenance technician Do not operate the unit in premises where condensate formation is possible DURING OPERATION Do not impede the platform motion Do not operate the unit in premises with aggressive or explosive chemical mixtures Please contact manufacturer for possibility of unit operation in specific atmosphere Do not use outside laboratory rooms Do not place a load exceeding the maximum load value mentioned in the Specifications section of this Manual Do not operate the unit if it is faulty or has been installed incorrectly BIOLOGICAL SAFETY It is the user s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment Page 3 2 General Information ES 20 60 Orbital Shaker incubator for biotechnological and pharmaceutical laboratories is a professional category equipment designed for cultivation of micro organisms cells and eukaryotic cells including animal plant and insect cells Shaker is equipped with a direct drive mechanism for platform motion It provides reliable and stable operation for the long term experiments needed for cell cultivation ES 20 60 Orbital Shaker incubator provides smooth or intensive mixing in flasks installed on the platform Built in thermoresistan
11. xitus PlusTM The table below shows the interreaction of Plexiglass with ethyl alcohol and other solutions Liquid Interreaction with Plexiglass DNA Exitus PlusTM No reaction RNase Exitus PlusTM No reaction Ethyl alcohol 10 15 No reaction Ethyl alcohol 30 Limited reaction Ethyl alcohol Pure Full reaction Do notuse H O 6 No reaction Fuse replacement Disconnect the power cord from electric circuit Disconnect the power cable from the socket on the rear of the shaker incubator Open the fuse holder Replace with the correct fuse KS for 230 V 50 60 Hz T3 15 A for 120 V 50 60 Hz S T5 0A i Fig 2 Fuse replacement Page 9 7 Warranty and Claims Tels 7 2 7 3 7 4 The Manufacturer guarantees the compliance of the unit with the requirements of Specifications provided the Customer follows the operation storage and transportation instructions The warranted service life of the unit from the date of its delivery to the Customer is 24 months Contact your local distributor to check availability of extended warranty If any manufacturing defects are discovered by the Customer an unsatisfactory equipment claim shall be compiled certified and sent to the local distributor address Please visit www biosan lv Technical support section to obtain the claim form The following information will be required in the event that warranty or post warranty service comes
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