Leica EZ5 Technical Brochure
Contents
1. 0 001 graduation A 10x focusing and framing graticule is available for photographic applications For models in which a graticule is included in an eyepiece for measurement or photography two eyepieces are necessary Graticules can be inserted in the adjustable eyepieces and the eyepieces for eyeglass wearers gt Use the stereomicroscope to determine the side on which the scale is inscribed The scale should not appear reversed gt Remove the insert from the bottom of the eyepiece and place it on the bench with the knurled side down User manual Leica EZ5 15 Hold the graticule by the edges to avoid leaving fingerprints and push it into the holder from the side gt Replace the insert in the eyepiece and press it firmly into place gt Insert the eyepiece in the tube and turn the eyepiece in the tube to align the graticule correctly The procedure for taking measurements is described in the Measuring operating instructions 16 User manual Leica EZ5 Control elements 1 Magnification changer right drive knob and functions of the with magnification scale standard equipment 2 Focusing knob 3 Fixing screw secures the optics carrier in the microscope carrier 4 Adjustable tubes Interpupillary distance adjustable from 55 75 mm 5 Eyepieces 6 Thread for lens protective glass figure shows ring illuminator User manual Leica EZ5 17 Requirements for working comfortably In2. Instruments Yet Leica symbolizes innovation as well as tradition Leica Microsystems an international company with a strong network of customer services Australia Gladesville Tel 61 2 9879 9700 Fax 61 2 9817 8358 Austria Vienna Tel 43 148680500 Fax 43 1 486 80 50 30 Canada Richmond Hill Ontario Tel 1 905 762 2000 Fax 1 905 762 8937 Denmark Herlev Tel 45 4454 0101 Fax 45 4454 0111 France Rueil Malmaison Tel 33 147328585 Fax 33 1 47 32 85 86 Germany Bensheim Tel 49 6251 136 0 Fax 49 6251 136 155 Italy Milan Tel 39 0257 486 1 Fax 39 0257 40 3475 Japan Tokyo Tel 81 3 5421 2800 Fax 81 3 5421 2896 Korea Seoul Tel 82 2 514 65 43 Fax 82 2 514 65 48 Netherlands Rijswijk Tel 31 70 4132 100 Fax 31 70 4132 109 People s Rep of China Hong Kong Tel 852 2564 6699 Fax 852 2564 4163 Portugal Lisbon Tel 351 21 388 9112 Fax 351 21 385 4668 Singapore Tel 65 6779 7823 Fax 65 6773 0628 Spain Barcelona Tel 34 93 494 95 30 Fax 34 93 494 95 32 Sweden Sollentuna Tel 46 8 625 45 45 Fax 46 8 625 45 10 Switzerland Glattbrugg Tel 41 44 809 34 34 Fax 41 44 809 34 44 United Kingdom Milton Keynes Tel 44 1908 246 246 Fax 44 1908 609 992 USA Bannockburn Illinois Tel 1 847 405 0123 Fax 1 847 405 0164 and representatives of Leica Microsystems in more than 100 countries In accordance with the ISO 9001 certificate Leica Microsystems Switzerland Ltd Business Unit Stereo amp Macroscope Systems
3. hands and turn them towards each other until the desired resistance is obtained when focusing The optics carrier can be turned sideways in the microscope carrier if the user wants to work from the side gt Loosen the clamping screw gt Turn the optics carrier to one side as required gt Tighten the clamping screw On the Leica EZ5 the magnification can be changed non incre mentally The magnification changer can be operated with the right or left hand A magnification scale has been provided on the right hand drive knob and is graduated with values from 1 to 5 The table on page 30 shows the magnifications and object field diameters relative to the position magnification changer and the eyepiece objective combination being used gt Look into the eyepieces gt Bring the object into focus page 19 gt Turn the magnification changer until the desired magnification has been set User manual Leica EZ5 Operation Setting diopters and If you set the diopters on the adjustable eyepiece exactly as parfocality described the image will remain equally sharp from the lowest to Preparatory work Focusing a test object the highest magnification This is referred to as parfocality and it means that you do not have to adjust the focus when the magni fication is changed You will only have to focus again when you want to view an object site at a higher or lower location Use this advantage as often possibl
4. has at its disposal a management system that meets the re quirements of the international standard for quality management In addition production meets the requirements of the international standard ISO 14001 for environmental management Winner 2005 Innovationspreis der deutschen Wirtschaft The World s First Innovation Award www leica microsystems com The companies of the Leica Micro systems Group operate international ly in three business segments where we rank with the market leaders Microscopy Systems Our expertise in microscopy is the basis for all our solutions for visuali zation measurement and analysis of micro structures in life sciences and industry With confocal laser techno logy and image analysis systems we provide three dimensional viewing facilities and offer new solutions for cytogenetics pathology and materi als sciences Specimen Preparation We provide comprehensive systems and services for clinical histo and cytopathology applications bio medical research and industrial quality assurance Our product range includes instruments systems and consumables for tissue infiltration and embedding microtomes and cry ostats as well as automated stainers and coverslippers Medical Equipment Innovative technologies in our surgi cal microscopes offer new therapeu tic approaches in microsurgery Sica MICROSYSTEMS ding and may be changed without notice
5. regulations must be obeyed User manual Leica EZ5 27 We guarantee the quality of our products 28 You are working with an extremely high performance precision instrument Accordingly we guarantee the quality of our instruments This guarantee covers manufacturing and material faults but not damage that has been caused by negligence or improper handling Please treat your valuable optical instrument with the care it deserves You will be rewarded with the function of a high perfor mance instrument which will deliver constant precision for de cades Our products are famous for this However if your instrument should not work perfectly please contact the approved technician your Leica representative or Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg directly User manual Leica EZ5 Calculating total magnification and object field diameter Mg magnification of the additional objective magnification of the eyepiece Z position of the magnification changer NFov field number of the eyepiece Field numbers are printed on the eyepieces 10x 20 16x 16 20x 12 10x 20B 16x 14B 25x 9 5B 40x 6B Example Mg additional objective 0 5x eyepiece 20 12 Z zoom position 5 0 Magnification in the binocular tube Mrorvis MgxMgxz 0 5x20x5 50 Object field diameter in the object Nrov 12 48mm Mg xz 0 5 5 User manual Leica 25 29 30 Optical data of the Leica EZ5 Working d
6. space requirement for use on bonders and in machine applications unobstructed access to specimens plenty of space for tools and a completely clear view of the object field The Greenough system enables cost effective correction of aber rations such as chromasia image field curvature and distortion with minimal effort The new Leica EZ5 uses the most corrected area of each lens for image production This provides superior optical performance with large level and undistorted fields of view and chromatically optimized high contrast images ESD protection The Leica EZ5 together with its cold light source and stand is made from highly conductive materials with area resistivity of 2x10 Ohm square Charges are cut from 1000V to 100V in less than 2 seconds Leica EZ5 with swing arm system User manual Leica EZ5 System diagram 13 312 611 1 Low eyepoint eyepieces High eyepoint eyepieces Eyepieces fixed Eyepieces fixed 10x 20 10 447 281 10x 20B 10 447 278 16x 16 10 447 132 16x 15B 10 447 138 20x 12 10 447 134 Eyepieces adjustable Eyepieces adjustable 10x 20 10 447 282 10x 20B 10 447 280 Leica EZ5 16x 16 10 447 133 16x 15B 10 447 139 10 447 201 20x 12 10 447 135 13 312 613 13 312 610 SS 10 446 372 10 446 390 10 447 100 13 312 614 10 447 104 10 447 259 LS 10 447 255 10 446 342 10 447 009 10 447 152 Q 10 446 392 e Sa 10 446 378 ESSEN 2 10 446
7. store any chemicals near your instruments e From improper treatment Optical systems and their mechanical parts must never be sepa rated unless such an operation is explicitly recommended in the instructions Fromoil and grease Do not grease guide surfaces or mechanical parts Place the dust cover over your instrument when you are not working with it Protect the tubus openings tubes without eyepieces and eyepieces with dust covers e Remove dust with a bellows and a soft paintbrush Clean eyepieces and objectives with special optics cleaning cloths and pure alcohol Store accessories in a dust free environment when they are not in use User manual Leica EZ5 Cleaning plastic parts Never clean How to clean with out damaging the equipment AN Several components are made of plastic or are plastic coated This makes them comfortable to hold and handle If these parts are cleaned improperly with unsuitable cleaning agents the plas tic may be damaged Therefore please follow these instructions nan ultrasound device The plastic may become brittle and over time may crack With caustic or acetone based media such as ether substitute e With other solvents with the exception of ethanol and isopropanol With warm soapy water then wiping down with distilled water With ethanol industrial alcohol and isopropanol If ethanol or isopropanol is used for cleaning the appropriate safety
8. 387 E 10 446 386 10 446 375 LJ 10447 008 Y Y Y n ae ay 10 447 006 10 446 377 10 446 436 10 447 260 10 447 007 10 User manual Leica EZ5 EZ5 optics carrier Eyepieces Objectives Carriers focus arms Focusing columns L2 waveguide Swing arm stands Leica L2 cold light source cO Cc c 4 CO N Flex arm accessories For detailed descriptions please refer to brochure M1 188 4en User manual Leica EZ5 Installing the basic equipment The components 1 Eyepieces fixed and or adjustable 2 Optics carrier Leica EZ5 3 Additional objective optional 4 Focusing column with microscope carrier 12 User manual Leica EZ5 Assembly Optics carrier Insert the optics carrier gently the gt Stand microscope carrier gt Fix the optics carrier in the desired position with the clamp screw Additional objective Screw the selected lens counterclock optional wise until it is seated firmly in place gt Optics carrier Protective Screw the protective glass cover firmly glass cover onto the thread directly on the Leica EZ5 optional This cover cannot be used at the same time as an additional objective Eyepieces Push eyepieces as far as they will go into Tubes the tubes gt Check that they are seated firmly and precisely in place You can use your Leica EZ5 together with a n fixed or adjustable eyepiece For models in which a graticule is included in an
9. Leica EZ5 User manual Sica MICROSYSTEMS Contents Safety concept Safety 6 Symbols eee 8 Description 9 Installation System diagram 1 0 0 0 cece cece eee eee eee 10 Installing the basic 12 ASSOMDIY cides ean ede ed pace dct d eae 13 15 Operation Operation cece eens 17 Special instructions What to do 0 RR 25 Maintenance Dd 26 Calculating total magnification and the object field 29 Optical data of the Leica 25 30 Dimensions of the Leica 5 31 Leica Microsystems around the world 32 User manual Leica EZ5 Dear User Thank you for your confidence in our product We wish you much enjoyment and success with your new EZ5 stereomicroscope from Leica Microsystems The new Leica EZ5 is an exciting addition to the extensive product range offered by our Swiss Stereomicroscopy business unit The Leica EZ5 has been designed specifically with a view to OEM appli cations In order to safeguard against ESD the entire stereomicro scope including the optional cold light source and the stand has been constructed with antistatic materials While developing the Leic
10. M2 120 6en Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg 2007
11. a EZ5 we took the greatest care to ensure that its operation would be simple and self explanatory Even so please take a little time to read the accompanying operating instructions This is the best way to familiarize yourself with all the features of your stereomicroscope and use it to its full advantage And if you have any questions please contact your Leica repre sentative or Leica Microsystems Switzerland Ltd Heerbrugg Switzerland We are gladly at your service CUSTOMER SERVICE is a big thing with us before and after the sale see page 36 Leica Microsystems Switzerland Ltd Stereo amp Macroscope Systems www stereomicroscopy com User manual Leica EZ5 3 Safety concept General notes Proper use Non intended use Place of use Integration ina third party product AN Read the operating instructions and safety notes before commissioning The Leica EZ5 is a precision optical instrument designed to facilitate visual examination of objects object details or speci mens in the fields of technology and science by magnifying them The equipment is supported by a full range of stands illumination systems and accessories Using the instrument in any way other than that described in these instructions may result in personal injury or damage to property The Leica EZ5 must not be used in examinations of or opera tions on the eye e Optical systems and mechanical parts must never be unscrewed from each
12. contact with the eyecups gt Place eyecups on the eyepieces If you wear eyeglasses for work gt Fold the eyecups backwards e In this way the eyecups will prevent your eyeglasses from being scratched In order to focus the operator uses the focusing drive to raise lower the stereomicroscope until the desired object site is in the focus working distance of the objective To see the working distances for the various objectives please refer to the table on page 30 The focusing drive can be operated with the right or left hand gt Place the object under the objective gt Set the lowest magnification You should set the lowest magnification because it is easier to find the desired object site in a large object field gt Look into the eyepieces gt Use the drive knob to bring the object into focus Note The instruments shown on this page are example illustrations only and may differ slightly from the actual appearance of the Leica EZ5 User manual Leica EZ5 19 Setting the ease of adjustment of the focusing drive Turning optics carrier sideways Zoom magnification changer Changing magnification 20 Is the focus movement too loose or too tight Does the outfit tend to slide downwards The force required to adjust the focusing drive can be increased or reduced depending on the weight of the equipment and the user s personal preference gt Hold the drive knobs in both
13. e it is not available on all stereomicro Scopes Diopters can be adjusted from 5 to 5 Each user needs to make these settings only once When a graticule is used diopters and parfocality are adjusted slightly differently and this procedure is described in the operat ing instructions for the graticules Measuring Set the diopter with an adjustable and a fixed eyepiece gt Set up the illumination system gt Adjust the interpupillary distance page 18 gt Use the focusing drive to set the working distance approxi mately the working distances of the various objectives are listed on page 30 gt Place a flat test object under the objective gt Set the lowest magnification gt Close the eye over the adjustable eyepiece and look into the fixed eyepiece with the other eye gt View the test object and use the focusing drive to bring it into focus User manual Leica EZ5 21 22 Setting the diopter on the adjustable eyepiece Checking parfocality gt Without looking into the eyepieces turn the eyelens on the eyepiece as far as it will go in the direction gt Close the eye over the fixed eye piece and look into the adjustable eyepiece with your other eye gt View the test object and slowly turn the eyelens clockwise towards until your eye sees the object in focus gt Set the highest magnification gt View the test object with both eyes _ and use the focusing dr
14. eyepiece for measurement or photography two eye pieces are necessary User manual Leica EZ5 13 Soft eyecups Cold light source Leica L2 gt Stand Illumination systems Eyepieces are supplied with soft eyecups which you can attach to the eyepieces in order to prevent eye infections if several users are working with the same instrument see page 6 protect your eyeglasses from scratching The Leica L2 cold light source and its glass fiber waveguides is the preferred illumination system for the Leica EZ5 stereomicroscope A number of match ing adapters are available for connect ing the Leica L2 cold light source to various stereomicroscope stands and for stand alone operation For detailed information about installation and use please refer to the operating instructions for the Leica L2 For more sophisticated requirements e g photography we offer a range of high performance transmitted light stands and low voltage illumination systems Ask your Leica sales representative for more details User manual Leica EZ5 Assembly Graticule gt Adjustable eyepieces The following graticules and stage micrometers for calibrating may be ordered e Graticule 10mm 0 1mm Graticule 5mm 0 1mm Graticule 5mm 0 05mm e Graticule 100 Div 0 002 e Graticule 100 Div 0 001 e Graticule 150 Div 0 0005 Crosshairs Stage micrometer 50mm 0 1 0 01mm graduation Stage micrometer 1
15. ica partner User manual Leica EZ5 Liquids Exercise particular care when handling liquids The instrument may be damaged by spilled liquids Legal requirements Adhere to general and local regulations relating to accident prevention and environmental protection Disposal The products described here must be disposed off in accordance with applicable local laws and regulations User manual Leica EZ5 Symbols gt You will see these symbols in the operating instructions Safety instructions This symbol indicates especially important information that must be read and complied with Failure to follow these instructions may endanger personnel Failure to follow these instructions may impair the system s performance or damage the instrument Important information This symbol indicates additional information or explanations that intend to provide clarity Action gt This symbol refers to actions described in the text that are to be carried out Explanatory notes This symbol indicates additional notes and explanations provided in the text User manual Leica EZ5 Description Greenough based The optical system of the Leica EZ5 consists of two beam paths optical system converging at 10 The lens pairs of each optical path are posi tioned close together so the stereomicroscope can be of very slender design especially towards the base of the instrument The advantages of this design are that it has a small
16. istance Zoom position magnification Object field Eyepieces magnification Object field magnification Object field 16x 15B 20 12 User manual Leica EZ5 Dimensions of the Leica EZ5 in mm 160 120 PI 55 75 0 63x additional objective 0 5x additional objective 21 8 180 mm image plane 140 mm lus _ WD image plane User manual Leica EZ5 Highest Focusing Position Interface for EZ5 Interface for Swing Arm Stand 10 447 255 Mountable focus arm User manual Leica EZ5 32 49 5 SSS i255 TN 25 Lowest Focusing gt Position Highest Focusing Position un 8 E e 37 7 10 446 344 Focus arm for 25 mm post User manual Leica EZ5 Interface for E75 _ 2 25 Hg Interface for 25 mm Column 33 34 71 0 5 Highest Focusing Interface for MZ5 Position Say 287 4x 24 5 holes M4 for Machine Interface 10 446 343 Focus arm User manual Leica EZ5 Leica Microsystems around the world Made by Leica Leica for you Leica Microsystems is active in the fields of microscopy sam
17. ive to opti mize the sharpness of the image Slowly move the magnification changer from the lowest magnification to the highest The sharpness should be constant parfocal If this is not the case repeat this procedure Note The instruments shown on this page are example illustrations only and may differ slightly from the actual appearance of the Leica EZ5 User manual Leica EZ5 Operation Preparatory work Focusing on the test object Set the diopter with two adjustable eyepieces gt Use the focusing drive to set the working distance approxi mately the working distances of the various objectives are listed on page 30 gt Set up the illumination system gt Adjust the interpupillary distance page 18 gt Set 0 diopters on both eyepieces gt Place a flat test object under the objective gt Set the lowest magnification gt View the test object through the eyepieces and use the focusing drive to bring the object into focus gt Set the highest magnification gt Use the focusing drive to optimize the sharpness of the image User manual Leica EZ5 23 24 Adjusting diopter settings Checking parfocality gt Set the lowest magnification gt Do not look into the eyepieces gt Turn the ocular eyelenses counter clockwise towards the as far as they will go gt Now look into the eyepieces gt Close one eye gt With the other e
18. mit ted to carry out repairs Only original Leica Microsystems spare parts may be used Responsibilities of Ensure that all staff who are responsible for operating the the person s equipment have read and understood these instructions in charge of particularly the safety notes Ensure that the Leica EZ5 is operated maintained and repaired only by authorized and trained staff User manual Leica EZ5 Safety concept A A Workplaces equipped with stereomicroscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Long periods of uninter rupted activity may cause asthenopic complaints and injury to the musculoskeletal system Appropriate measures should be adopted to reduce physical stress e optimal arrangement of workplace work assignments and work flow changing tasks frequently e thorough training of the personnel giving consideration to ergonomic and organization aspects The ergonomic optics concept and the design of the Leica EZ5 aim to limit the strain on the user to the lowest possible level Direct contact with eyepieces is a potential transmission method for bacterial and viral infections of the eye Users should be made aware of the potential risk of infection This risk can be minimized by using personal eyepieces or removable eyecups see page 19 Eyecups can be purchased separately For more information contact your Le
19. other unless expressly indicated in the instructions The Leica EZ5 is primarily intended for use in enclosed premises fitis used outdoors the Leica EZ5 must be protected from dust and moisture Electrically powered illumination systems and stands produced by Leica Microsystems must not be used outdoors When installing Leica products into third party products note the following The manufacturer of the complete system or its dealer is responsible for following all applicable safety instructions laws and guidelines User manual Leica EZ5 Use in clean The Leica EZ5 is suitable for use in clean rooms and can be pre rooms pared for such use as described on page 27 Please observe the following rules e Never use any procedure other than the one described in these operating instructions to clean the Leica EZ5 and its acces sories Do not use unsuitable cleaning agents chemicals or equipment Never use chemicals to clean colored surfaces and accessories with rubberized parts This may damage surfaces and abraded particles may contaminate preparations e fa user uses chemicals to clean any Leica instrument without our written approval he does so at his own risk We will be glad to offer special solutions upon request that are sufficient for most circumstances Some products can be modified or we can offer different accessories for use in clean rooms Servicing Only Leica Microsystems trained service technicians are per
20. ple preparation image analysis confocal laser equipment medical equipment and equipment for the semiconductor industry The international technology group which is headquartered in Wetzlar Germany de veloped from a group of companies with proud individual traditions Wild Leitz Reichert Jung and Cambridge Instruments Leica Microsystems develops system solutions for enhanced customer benefit and innovative leading edge technologies to standards that command international respect The same quality standards are applied in all of our 11 production facilities in 7 countries Leica is thus synonymous with quality the world over Technology is one side of the Leica Microsystems success story the other is customer care Our staff provide cooperative professional consulting service and customer care on site and in our customers national languages We also pride ourselves on our ability to pro vide reliable uncomplicated solutions to our customers needs and a flawless delivery program User manual Leica EZ5 35 Leica Microsystems the brand for outstanding products Leica Microsystems mission is to be the world s first choice provider of innova tive solutions to our customers needs for vision measurement and analysis of micro structures Leica the leading brand for microscopes and scientific instruments developed from five brand names all with a long tradition Wild Leitz Reichert Jung and Cambridge
21. terpupillary distance exit pupil Make sure that your Leica EZ5 is adjusted correctly You must make all the settings described here precisely in order to be able to take full advantage of its outstanding optical and ergo nomic advantages e Set your work area up for the best possible conditions Consider the height of the bench and chair e Use the whole seat surface and the backrest Ensure that your lower arms are supported When carrying out other tasks perform exercises to relax and relieve muscle tension The Leica EZ5 with a 60 viewing angle offers optimum viewing height on the inclined stereomicroscope The interpupillary distance is adjustable in a range from 55 75mm The exit pupil is the distance between the eye and the eyepiece It is 12mm for standard eyepieces 10x 20 16x 16 and 20x 12 fixed and adjustable 22mm for eyepieces 10x 23B and 16x 15B fixed and adjustable and also for the wide field eyepieces for eyeglass wearers 10x 21B 16x 14B 25x 9 5B and 40x 6B gt Move your eyes slowly towards the eyepieces 55 and push the tubes together or apart with two ON hands until you see a single round image field SS with no shadowing with both eyes 5 User manual Leica EZ5 Operation Eyecups Adjust working distance focusing creating a clear image Eyepieces are supplied with detachable eyecups see also page 8 If you do not wear eyeglasses and want close
22. ye view the test object and slowly rotate the eye lens clockwise in the direction until the object appears in focus to that eye gt Adjust the diopter for the other eye in the same way gt Select the highest magnification gt Look at the object and gently adjust the focus if necessary gt Slowly move the magnification changer from the lowest magnifi cation to the highest e The sharpness should be constant for the entire zoom range parfo cal If this is not the case repeat this procedure Note The instruments shown on this page are example illustrations only and may differ slightly from the actual appearance of the Leica EZ5 User manual Leica EZ5 What to do if There is a shadow on the object field e Adjust the interpupillary distance correctly page 18 The image does not become sharp e Insert the eyepieces correctly page 13 Carry out diopter correction exactly as described in the instructions page 21 The focusing drive gradually sinks on its own or is difficult to turn Adjust the ease of movement page 20 User manual Leica EZ5 25 Maintenance 26 Protect your instruments Dust and dirt will affect the quality of your results In the following section we will describe the proper care of your valuable instrument and provide a few hints on maintenance and cleaning From moisture dust acids bases and caustic substances Do not
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