Home

Copyright

1. Figure 63 Power switch on external 355 nm UV laser Figure 64 Power switch on external 405 nm UV laser 16 Activate the following key switches to switch on the external 355 nm and 405 nm UV laser Figure 65 Key switch on the power supply of external 355 nm UV laser 05 Figure 66 Key switch on the external 405 nm UV laser 17 After the workstation has started log on to the operating system Using a personalized user ID Use your personal user ID if one has been set up This ensures that user specific settings are saved and maintained for this user only If the system administrator has not yet assigned a personal user ID log on as CS User The default password is True Confocal55 Change password After logging on with your own user ID you may change your password To do so press the Ctrl Alt and Del keys at the same time Then click Change password The Change password dialog box opens Now you can start the LAS AF software 122 TCS SP8 with Compact Supply Unit an Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 1 Switch on the cooling for the infrared laser see Figure 67 item 1 The co
2. 25 EF VCS SPS ete hebdo E das 25 1 1 1 TCS SP8 with Upright Microscope and Compact Supply Unit 25 1 1 2 TCS SP8 with Upright Microscope and Flexible Supply Unit 27 1 1 3 TCS SP8 with Inverted Microscope and Compact Supply Unit 28 1 1 4 TCS SP8 with Inverted Microscope and Flexible Supply Unit 29 12 TCS SPe MP System Variants seduce YA Pr aE EIER Po Ste eR aca 30 1 2 1 TCS SP8 MP with DM6000 and IR Laser without Dispersion Compensation 30 1 2 2 TCS SP8 MP with DM6000 and IR Laser with Dispersion Compensation 31 1 2 3 TCS 5 8 MP with DMI6000 and IR Laser without Dispersion Compensation 32 1 2 4 TCS SP8 MP with DMI6000 and IR Laser with Dispersion Compensation 33 1 2 5 TCS SP8 MP with DMI6000 and InSight DeepSee laser 34 13 TCS SPSXSYStEM i323 2 RW a aba etre Ed EE 36 7 3 1 TCS SP8 X with Upright Microscope 36 7 3 2 TCS SP8 X with Inverted 37 74 TOS SPOowil External DV Tase iet eT 38 75 Controls on the Supply Unit cse eh ve ae ea EE EN A 38 1 5 1 Main Switch Board on the Flexible Supply 38 1 5 2 Control Panel Field on the Compact Supply Unit 39 To 40 1 6 1 DIMENSIONS
3. Figure 97 Key switch on the external 405 nm UV laser 9 Switch off both external 355 nm and 405 nm UV laser main power switches Figure 98 Power switch on external 355 nm UV laser Figure 99 Power switch on external 405 nm UV laser 10 If you are using a HyD RLD switch it off at its supply unit Figure 100 HyD RLD supply unit 111 11 If your system has an infrared laser MP or an optical parametric oscillator OPO switch off the NDD detection unit Figure 101 NDD detection unit 12 Switch off the microscope s control panel Figure 102 Control panel for the microscope 13 Now shut down the workstation 14 Switch off the lasers see Figure 93 item 3 the scan head see Figure 93 item 2 the workstation and the microscope see Figure 93 item 1 on the flexible supply unit s main switch board 15 Turn off any accessories being used Observe the user manuals for external lasers BA Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 112 17 2 TCS SP8 with Compact Supply Unit Observe the user manuals for external lasers BA Please re
4. Figure 4 TCS SP8 with upright microscope and flexible supply unit 1 Upright microscope 6 Flexible supply unit 2 Scan head 7 Control panel 3 SmartMove 8 Fluorescence lamp EL6000 4 Workstation 9 Microscope electronics box 5 Main switch board Figure 5 Dimensions of TCS SP8 with upright microscope and flexible supply unit 2 28 1 1 3 TCS SP8 with Inverted Microscope and Compact Supply Unit Figure 6 TCS SP8 with inverted microscope and compact supply unit 1 Inverted microscope 5 Control panel 2 SmartMove 6 Fluorescence lamp EL6000 3 Workstation 7 Microscope electronics box 4 Compact supply unit 8 Scan head Figure 7 Dimensions of TCS SP8 with inverted microscope and compact supply unit 7 1 4 TCS SP8 with Inverted Microscope and Flexible Supply Unit Figure 8 TCS SP8 with inverted microscope and flexible supply unit 1 Inverted microscope 6 Control panel 2 SmartMove 7 Fluorescence lamp EL6000 3 Workstation 8 Microscope electronics box 4 Main switch board 9 Scan head 5 Flexible supply unit Figure 9 Dimensions of TCS SP8 with Inverted Microscope and Flexible Supply Unit 29 30 1 2 1 2 1 TCS SP8 MP System Variants TCS SP8 MP with DM6000 and IR Laser without Dispersion Compensation 130 cm 4 3 90 cm 2 11 440 cm 14 5 Figure 10 System overview of the TCS SP8 MP with DM6000 and IR laser without dispersion compensation 1 St
5. 49 8 6 3 TCS SP8 MP with flexible supply 49 0 6 4 TCS SP8 MP with compact supply unit 49 8 6 5 CEDERE ETHER 50 0 6 6 External oe oam tonne tek buted oe a OD Cer ead ees 50 Ella Fi edad pout eae wae eee he wae taint a eee te eae ee 51 JE SeS auditi ted ista etd eee Et old teque MEA 51 9 2 Overview of Usable 51 9 2 1 VIS UV Lasers TOF CSS PO vw Lice iac ac ER XXE LES papa ee 51 9 2 2 VIS UV Lasers Tor TCS SP8 X cc ges ROI aC CASU 52 9 2 3 IF Easers Tor CS SP9 ori hr rr ah oe ot oe ded ir n d Dci e C e cn 52 9 2 3 1 Picosecond 52 9 2 3 2 ERE EDI EIE e EUER ed 53 10 Safety Features 0 x3 AE 55 10 1 Main Circuit Breaker for Disconnecting the Power Supply 55 10 1 1 Compac Supp EIE oid ence c dod ee doi de 55 101 2 Flexible Supply Ult iie oe Ebk E Rb E Ox rpiDenir crE n eb ER PIRE be 55 10 2 KEY OWI ioa sna ea waned ee aa wean s DR e e Se 56 10 2 1 Master Key Switch on the Compact Supply 56 10 2 2 Master Key Switch on the Flexible Supply 56 10 23 Key Switch for the White Light 57 10 2 4 Key Switch fo
6. she ob r Shine ad xtd eias eit 40 1 6 2 Weight of the Supply 40 1 6 3 Electrical 5peciflC allons a ii cure o RC d eec ca is 41 1 Electromagnetic Compatibility 0 0 ccc cece eee eee ees 41 HO Sera Numbi Lad du nice E ACA DR aci xd S 42 Ambient 43 0 1 General Requirements Regarding Ambient Conditions 005 43 92 UMIBESUOFIS Patto dps do ud EM I LL E Le er E Ud 43 0 3 ROOM DIMENSIONS aab eed ae cer ae RES m Ra DOG a eaten 44 0 4 Electrical Connection Requirements 45 8 4 1 TCS 5 8 with flexible supply unit 45 8 4 2 TCS SP8 with compact supply 45 8 4 3 TCS SP8 MP with flexible supply 46 8 4 4 TCS SP8 MP with compact supply unit 46 8 4 5 MGSO 25 PEUT 46 0 4 6 External MASCIS uses RUE PED CRX OTT pP ERE 4 8 5 Load capacity of the multiple socket outlet on the flexible supply unit 4 6 6 Waste Heal and Goong iex xerox eap E Rd crei e HA bw ei re 48 8 6 1 TCS SP8 with flexible supply unit 48 8 6 2 TCS SP8 with compact supply
7. infrared laser for a longer period of time you can shut it off completely To shut the InSight DeepSee off completely call up the MP dialog see Figure 90 item 1 and then click on the button Laser shutdown Figure 90 item 2 In this case you have to deactivate the supply unit for the infrared laser Figure 91 item 1 as well After 15 minutes you can also switch off the cooling for the infrared laser Figure 91 item 2 v Current Laser Values Wavelength 1150 nm Output Power 1 24 W Y Configuration Acquire v Adjust Laser 680 nm 1300 nm Load Save single setting User Settings 107 1150 nm Status Shutter Tuneable Shutter 1040nm Laser Shutdown 9 4j i 5 20x 0 75W ready Figure 90 Laser shutdown for switching off the InSight DeepSee laser 1 Figure 91 Cooling and supply unit of the InSight DeepSee infrared laser 108 3 4 D Ifthe argon laser is operating you have to deactivate it before closing LAS AF in the laser configuration Do not continue with the switch off sequence until the argon laser ventilation has automatically shut off after approx 3 minutes NOTICE Damage to the instrument when not adhering to the switch off sequence If LAS AF is closed without deactivating the argon laser in the laser configuration ventilation will not be shut off automatically Never shut off the hardware components by means of the flexible
8. innovatek OS GmbH Dated www innovatek de February 28st 2011 innovatek Protect application mixture info innovatek de Upper explosion limit 15 0 vol only valid fur the Ethylenglykol part Ignition point gt 200 C DIN 51794 only valid f r the Ethylenglykol part Vapor pressure 20 C 2 mbar DIN 51757 Density 20 C ca 1 04 g cm Miscibility with water optional mixable Solubility quantitative solvent polar solvents soluble Viscosity kinematic 20 C 3 5 mm s 10 Stability and reactivity Substances to avoid Strong oxidizing agents Hazardous reactions No hazardous reactions when stored and handled according to instructions Hazardous decomposition No hazardous decomposition products if stored and handled products as prescribed indicated 11 Toxicological data LD50 oral rat 2000 mg kg only valid f r the Ethylenglykol part LD50 dermal rabbit non irritant only valid f r the Ethylenglykol part Primary skin irritation rabbit non irritant Information on Ethylene glycol Further information Developmental toxicity was observed after oral ingestion of high doses in studies with rats and mice but this effect was not seen in a study with rabbits Experiences in humans Lethal dose if swallowed approx 1 5 g kg body weight Lethal dose approx 90 110 g for adults and correspondingly less for children Smaller doses can result in consciousness is affec ted kidney damage dama
9. 10 grounded Frequency 50 60 Hz Power connection Three separate electric circuits with Power supply voltage 100 V 120 V fuse protection 20 A Or Power supply voltage 200 V 240 V fuse protection 12 16 A Two for the flexible supply unit s power supply and one for supplying peripheral devices To prevent damage to the instrument the two types of voltage 200 to 240 V and 100 to 120 V must not be combined Depending on the system configuration further electrical circuits may be required Power supply for USA Two terminals of the NEMA 5 20 type for the supply unit Terminals of the NEMA 5 15 type for the remaining instruments 8 4 2 TCS SP8 with compact supply unit Supply voltage 100 V to 240 V 10 grounded SEIT Power connection Two separate electric circuits with Power supply voltage 100 V 120 V fuse protection 20 A Or Power supply voltage 200 V 240 V fuse protection 12 16 A One for the compact supply unit s power supply and one for supplying peripheral devices To prevent damage to the instrument the two types of voltage 200 to 240 V and 100 to 120 V must not be combined Depending on the system configuration further electrical circuits may be required Power supply for USA Terminals of type NEMA 5 15 45 8 4 3 TCS SP8 MP with flexible supply unit Supply voltage 100 V to 240 V 10 grounded Frequency 50 60 Hz Power connection Four separate electric circuits with Pow
10. Living up to Life User Manual Leica TCS SP8 Leica TCS SP8 MP Leica TCS SP8 X Published by Leica Microsystems CMS GmbH Am Friedensplatz 3 D 68165 Mannheim Germany http www leica microsystems com http www confocal microscopy com Responsible for contents Leica Microsystems CMS GmbH Copyright Leica Microsystems CMS GmbH All rights reserved Copyright All rights to this document are held by Leica Microsystems CMS GmbH Adaptation translation and reproduction of text or illustrations in whole or in part by print photocopy microfilm or other method including electronic systems is not allowed without express written permission from Leica Microsystems CMS GmbH Programs such as LAS and LAS AF are protected by copyright laws All rights reserved Reproduction adaptation or translation of these programs is prohibited without prior written permission from Leica Microsystems CMS GmbH AOBS is a registered trademark of Leica Microsystems CMS GmbH This User Manual specifies names of products or services that are trademarks or registered trademarks of the respective trademark owners Rather than including a trademark TM or symbol at every occurrence of a trademarked name we state that we are using the names only in an editorial fashion and to the benefit of the trademark owner with no intention of infringement Made in Germany Copyright Leica Microsystems CMS GmbH All rights reserved Copy
11. UV external 355 lt 500 18 Continuous wave cw Ar 458 476 488 200 lt 50 Continuous wave 496 514 cw White light laser 470 670 Pulsed IR Lasers for TCS SP8 MP The TCS SP8 MP can include a combination of all of the lasers listed in Chapter 9 2 1 and Chapter 9 2 2 except for the external UV laser 355 nm and the following IR lasers Picosecond Laser Laser type Wavelength Maximum Maximum Pulse duration nm light outputat light output in laser output focal plane W W MaiTai HP ps 690 1040 Pulsed 1 0 1 5 ps Chameleon Ultra Il ps 680 1080 Pulsed 1 0 1 5 ps 9 2 3 2 Femtosecond laser Laser type MaiTai fs MaiTai HP fs MaiTai HP DeepSee MaiTai eHP DeepSee InSight DeepSee Chameleon Ultra fs Chameleon Ultra II fs Chameleon Vision 11 Chameleon Vision S Chameleon Ultra 11 Compact OPO Chameleon Vision ll Compact OPO Wavelength Maximum Maximum Pulse duration nm light outputat light output in laser output focal plane W W 5 3 1 3 1 680 1080 lt 4 0 pulsed 140 fs 1000 1280 pulsed gt 100 fs 690 1050 19 pulsed 75 fs 53 54 10 10 1 10 1 1 10 1 2 Safety Features The Leica TCS SP8 system is available with a flexible or a compact supply unit Note the safety features described here that apply to your supply unit Main Circuit Breaker for Disconnecting the Power Supply Compact Supply Unit In order to sw
12. A navigation window is on the left side of the screen in the LAS AF Online Help It contains three tabs that allow the contents of Online Help to be retrieved in different ways Contents tab Contains the table of contents in the form of a directory tree that can be expanded or collapsed Double click an entry in the table of contents to display the corresponding information Index tab Displays all keywords alphabetically and hierarchically Double click on an index entry to display the corresponding information or press the Display button You can also search by keyword Enter the word you would like to search for in the search box Online help displays the keyword that is the closest match to the specified word 13 3 5 e Search tab Allows full text searching Enter the word you would like to search for in the search box Click on the List Topics button An alphabetically structured list of topics containing the keyword is displayed Full text Search with Logically Connected Search Terms In Online Help you can use the full text search function to search for words and for phrases enclosed in quotation marks You can also use placeholders when searching such as or You can run a full text search using logical operators such as boolean operators in order to link search terms and thereby increase the accuracy of search results In the input field on Online Help s Search tab directly input the desired logical operator togeth
13. Ambient Conditions Here you can find a summary of the information regarding the size and design of the room and the general requirements regarding ambient conditions Be absolutely sure to comply with the ambient conditions General Requirements Regarding Ambient Conditions You may use the system only in indoor areas that are dust free The room must be free of dust oil and chemical vapors Avoid direct sunlight and vibrations since these can distort measurements and micrographic scans We also recommend using a room that can be completely darkened The room must meet national safety regulations for laser safety areas Never expose the system to rain fluids or humidity Do not set up the system under water pipes air conditioning systems or other piping Otherwise this could cause fire and electrical shocks to the system and the electrical components The room should be equipped with a telephone connection to contact Leica Microsystems CMS GmbH for phone support For RemoteCare an Internet connection is also needed The system should be set up in a separate room in order to be able to provide a constant temperature and appropriate ambient lighting without having to address needs of other systems instruments and room users Temperature range for 18 to 25 C 64 to 77 F operation optimum optical behavior 22 C 1 C 72 F 1 8 F Pollution degree 2 protect system against dust Permitted relative humidity 20 to
14. When not in use cover the system with a plastic cover or a clean piece of cotton cloth Avoid condensation Allow the entire system to cool down to room temperature before 1 covering the system with a dust cover This prevents condensation from forming below it which can enter the system and damage it 19 Repairs and Service Work WARNING Contamination with hazardous substances Before each call from a service technician the system has to be cleaned thoroughly to avoid contamination with hazardous biological materials A When returning system parts it must be ensured that they are free of hazardous substances otherwise they must not be sent This applies in particular to systems that are located in biomedical research labs Repairs and servicing may be performed only by service technicians authorized by Leica Microsystems CMS GmbH Opening or working on the system in any way shall void any and all warranty claims e f housing parts have to be opened for repairs or service work only Leica service technicians may be present in the room where the system is installed Be sure to back up your data before any service or repair work is performed Leica Microsystems CMS GmbH shall not be liable for any loss of data e Inside the system there are installed components that can cause potentially fatal injury if handled improperly Opening these components will result in danger to people and the system Therefore only authorized Leic
15. for white light laser Covered design 50 60 Hz You can find more information on electrical connection requirements in Chapter 8 4 and in Chapter 8 5 Observe the user manuals for external lasers BA Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes Electromagnetic Compatibility In regards to emitted interference this is a class A system CISPR 11 This system is suitable for use in buildings that do not include domestic premises and buildings not directly connected to a low voltage power supply network that supplies buildings used for domestic purposes The system can cause radio interference in a household environment In these cases the operator may have to take measures to eliminate the interference When using internal and external HyDs it is recommended that the system only be operated in a controlled electromagnetic environment This is because the use of cell phones or other radio transmitting devices such as DECT phones can cause picture interference if used in the immediate vicinity of the system 41 42 78 Serial Number The serial number for your system is located on the rear side of the scan head eE Se Cw 4 Tii Ce 9 rd C gt Figure 23 Rear side of the scan head label with serial number 8 1 8 2
16. 2006 standard Note The actual product may or may not include all the part types listed above 139 140 www leica microsystems com CLCCL MICROSYSTEMS Leica Microsystems CMS GmbH Phone 49 621 7028 0 Am Friedensplatz 3 Fax 49 621 7028 1028 D 68165 Mannheim Germany http www leica microsystems com Copyright Leica Microsystems CMS GmbH All rights reserved User manual Leica TCS SP8 Leica TCS SP8 MP I Leica TCS SP8 X Order No 158000002 V 01
17. LAS AP UnlIhe Help 2x aids acto OE OC d de Ce UB ens Sd e DOCU aea ec e E s 97 13 3 1 SCUCHIFG OF ONIMG Help eco re ptos eeebeotut dus 97 13 3 2 Accessing Online 1 97 13 3 3 Selecting the Language for Online 98 B34 Using Online wr ER uta CERE e CREER RC xD eo 98 13 3 5 Full text Search with Logically Connected Search Terms 99 14 Changing the Specimen seeueeeeeen I IH 101 14 1 Changing the Specimen on an Upright Microscope 101 14 2 Changing the Specimen on an Inverted Microscope 101 15 Changing the Objective LLslulseeeee nnn n IK e eens 103 16 Piezo Focus on Upright 105 17 Switching Off the System 0 ccc cece eee eee eee n nnne 107 17 1 TCS SP8 SP8 X with Flexible Supply 107 17 2 TCS SP8 with Compact Supply 113 19 Care and Gleanings eec E Ea LEYES 119 19 1 leaning SUMACES x 55 ep wien bor e dor iR RUE c ER b ans 119 18 2 Cleaning tie Optical System co po eb c Pop SS 119 18 3 Gleaning Immersion Lenses uot ER CDD Peete we sea XE 120 10 4 RUE 120 19 Repairs and Ser
18. electric circuits with Power supply voltage 100 V 120 V fuse protection 20 A Or Power supply voltage 200 V 240 V fuse protection 12 16 A Two for the flexible supply unit s power supply and one for supplying peripheral devices To prevent damage to the instrument the two types of voltage 200 to 240 V and 100 to 120 V must not be combined Depending on the system configuration further electrical circuits may be required Two terminals of the NEMA 5 20 type for the supply unit Terminals of the NEMA 5 15 type for the remaining instruments Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser Wn manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes Load capacity of the multiple socket outlet on the flexible supply unit The total power consumption of all loads connected to the multiple socket outlet see Figure 24 must not exceed 800 VA The terminals are intended for e Workstation e Monitor e Microscope 4 48 8 6 8 6 1 Figure 24 Load capacity on the rear side of the multiple socket outlet on the flexible supply unit Waste Heat and Cooling NOTICE Instruments may sustain damage Never install the system close to air conditioners or other ventilation systems Air from air conditioning systems or other ventilation equipment should not blow directly on the microscope Us
19. if you need to move or transport the system or need to ship parts of It WARNING Contamination with hazardous substances Before each call from a service technician the system has to be cleaned thoroughly to avoid contamination with hazardous biological materials A When returning system parts it must be ensured that they are free of hazardous substances otherwise they must not be sent This applies in particular to systems that are located in biomedical research labs Follow the notes provided in Chapter Repairs and Service Work 125 Disassembly and Transport 126 22 Disposal Atthe end of the product service life please contact the Leica branch office in your country with regard to disposal Disposal The system its accessory components and consumable materials must not be disposed of together with general household waste Be sure to follow the national laws and regulations 127 128 23 Contact If you have any further questions please directly contact your country s Leica branch office or your local contact person The appropriate contacts can be found on the Internet under http www confocal microscopy com 129 130 24 24 1 24 2 Appendix Patents The Leica TCS SP8 product is protected by US patents 5 886 784 5 903 688 6 137 627 6 222 961 6 285 019 6 311 574 6 355 919 6 423 960 6 433 814 6 444 971 6 466 381 6 510 001 6 614 031 6 614 525 6 614 526 6 654 165 6 657 187 6 6
20. lasers are in the on position The master key switch for the compact supply unitis located on the front side of the unit see Figure 26 Scanner Laser Laser Power Power Emission 6J 6J a Figure 26 Master key switch on the compact supply unit Master Key Switch on the Flexible Supply Unit There is a master key switch on the flexible supply unit for protection against unauthorized use of the laser products This master key switch is integrated in the interlock circuit If the master key switch is in the off position all laser beam paths are interrupted Then no radiation from lasers integrated in the supply unit or the external lasers can reach the specimen area even if the key switches of the external lasers in the on position The master key switch for the flexible supply unit is located on the front side of the main switch board see Figure 27 L On 1 OOo Scanner Laser Microscope Power Power Emission Figure 27 Master key switch on the main switch board of the flexible supply unit 10 2 3 10 2 4 Key Switch for the White Light Laser The external lasers can also be turned off individually The detachable key switch for protection against unauthorized use of the external white light laser is located on the front of the white light laser see Figure 28 Figure 28 Key switch for the white light laser Key Switch for UV Laser
21. microscope safety switch is integrated in the interlock circuit T EXD S S D E CTE a fod Jo Figure 39 Position of the interlock connector on the scan head 63 64 10 5 10 6 Safety Switches on the Microscope When the safety switches are triggered the light path of the laser beam is interrupted Figure 40 Safety switches 1 and 2 on the inverted microscope left Safety switches 3 on the mirror housing for the upright microscope right Position of Is triggered the safety by switch Transmitted light illuminator arm Motorized changeover from eyepiece mode to scan mode Pushbutton in the port apertures of the mirror housing Warning Messages Is triggered if The illuminator arm is tilted e g for working on the specimen The path folding mirror for the scan head is swung out by motor One of the two apertures on the mirror housing is free no external detector no halogen lamps no cover Function Prevents laser light while working on the specimen Prevents stray light in the eyepiece if the user switches from eyepiece observation to confocal observation Prevents the escape of laser light if the user removes components from the mirror housing Warning messages in the event of a defective shutter The shutters are monitored for safety If one of the shutters is defective or notfunctioning properly a warn
22. nm Load Save single setting User Settings 1150 Status Shutter Tuneable Shutter 1040nm 20x 0 75W ready Figure 104 Laser shutdown button for switching off the InSight DeepSee laser 1 Figure 105 Cooling and supply unit for the InSight DeepSee infrared laser 3 Ifthe argon laser is operating you have to deactivate it before closing LAS AF in the laser configuration Do not continue with the switch off sequence until the argon laser ventilation has automatically shut off after approx 3 minutes NOTICE Damage to the instrument when not adhering to the switch off sequence If LAS AF is closed without deactivating the argon laser in the laser configuration ventilation will not be shut off automatically Never shut off the hardware components by means of the flexible or the compact supply unit before the shut off argon laser has cooled down over the ventilation since it can incur damage otherwise 4 Close the LAS AF software On the menu bar select File Exit 114 File Help New Experiment Open Experiment Close Experiment Print Print Setup Print Preview Figure 106 Shutting down LAS AF 5 Turn off the lasers in the supply unit with the key switch on the front side of the compact supply unit see Figure 107 item 3 Scanner Laser Laser Power Power Emission H el 3 Figure 107 Overview of the Control Panel Field on the Compa
23. not operate the system if coolant is leaking or has leaked Malfunction of the System You must immediately disconnect the system from the power supply if any of the following occur The emission warning indicator is not lit after being switched on using the detachable key switch The indicator continues to be lit after being switched off using the keyswitch Scanning of the specimen is not activated after being switched on properly laser radiation in the specimen area If any of these occur immediately notify the Leica branch office in your country or your local contact person 6 1 6 2 Additional Notes on Handling the System Follow these instructions to ensure that you handle the system without interference to avoid damage to the instrument and loss of data Location You need sufficient space for temporary storage and for unpacking the delivered components Always protect the transport crates and their contents from moisture and condensation and store them facing upwards see the indication on the crate Upon receiving the crates make sure they are intact If you find that the crates or seals have been damaged have the supplier confirm this inform your contact person at Leica Microsystems about this immediately Keep the packaging material in case you need to return a defective component Be absolutely certain to observe the ambient conditions applicable for this system You may use the system indoors only The ro
24. or incinerated in accordance with local regulations Contaminated Uncontaminated packs can be reused Packs that cannot packaging be cleaned should be disposed of in the same manner as the contents 14 Transport information Not dangerous according to transport regulations ADR RID ADNR IMDG GGVSee ICAO IATA 15 Regulatory information Regulations of the European union Labelling National legislation Regulations Directive 1999 45 EEC Preparation Directive Hazard Symbol Xn Harmful R phrases 22 Harmful if swallowed S phrases 2 Keep out of reach of children 24 25 Avoid contact with skin and eyes 46 If swallowed seek medical advice immediately and show this container or label Hazard determinant component for labelling 1 2 Ethanediol Other regulations Classification according to VbF Germany None Categorization according to TA Air Germany 3 1 7 Category Ill Water compromises category attachment 4 of VwVwS Germany from 17 Mai 1999 1 low water compromising 16 Further Information Full text of hazard symbols and R phrases if mentioned as hazardous components in chapter 2 Xn Harmful 22 Harmful if swallowed 63 Possible risk of harm to the unborn child Vertical lines in the left hand margin indicate an amendment from the previous version This safety data sheet is intended to provide information and recommendations as to 1 how to handle chemical substances and preparations in acc
25. protective eyewear when using VIS and UV systems When used as intended and safety notes have been followed laser radiation is kept within the limit value that eliminates the chance for eye injuries Do not use an S70 microscope condenser The large working distance and the low numerical aperture of the S70 microscope condenser could result in a hazard from laser radiation Only use 1 and S28 Leica microscope condensers Do not look into the eyepieces during the scanning operation Never change samples during a scanning operation Never change objectives filter cubes beam splitters condensers or other components during a scanning operation Do not look into the eyepieces when switching the beam path in the microscope Do not introduce any reflective objects or mirrors into the laser beam path or into the specimen area If there is no lamp housing or mirror housing connected to the microscope attach the cover to the replacement flange All unoccupied positions in the objective nosepiece must be closed using the supplied caps For MP systems dry objectives air objectives may not be used with a numerical aperture NA larger than 0 85 This does not apply to immersion objectives oil water Electrical Safety This system is designed for connection to grounded earthed outlets The grounding plug performs an important safety function To avoid the risk of electrical shock or damage to the instrument do not disable this featu
26. the Control Panel Field on the Compact Supply Unit 10 Switch on the lasers on the front side of the compact supply unit see Figure 73 item 2 The system s power supplies and fans are started 11 If your system has an infrared laser MP or an optical parametric oscillator OPO switch on these components as described in the manufacturers respective user manual 12 To switch on the laser in the supply unit press the key switch on the front side of the compact supply unit see Figure 73 item 3 WARNING _ Risk of permanent eye and skin damage from laser radiation From this time on laser radiation may be present in the specimen area of A the laser scanning microscope Make sure to follow the safety notes for operation of the system 13 If you are using external 355 nm UV laser including 405 nm wavelength make sure that the power switch is in the position Figure 74 Power switch on external 355 nm UV laser 89 Figure 75 Power switch on external 405 nm UV laser 14 Activate the following key switches to switch on the external 355 nm UV laser including 405 nm Figure 76 Key switch on the power supply of external 355 nm UV laser Figure 77 Key switch on the external 405 nm UV laser 15 After the workstation has started log on to the operating system Using a
27. the Optical System e Never open the objectives for cleaning e Remove dust with a fine dry brush made from hair or with a clean lint free cloth moistened with distilled water e Prevent the optics and mechanical parts from coming into direct contact with acids bases and other aggressive chemicals e Remove persistent dirt from glass surfaces using pure alcohol or chloroform 119 120 18 3 18 4 Cleaning Immersion Lenses The immersion oil should be removed from oil immersion lenses immediately after it is applied 1 2 First remove the immersion oil using a clean cloth Once most of the oil has been removed with a clean tissue a piece of lens tissue should be placed over the immersion end of the lens Apply a drop of the recommended solvent Gently draw the tissue across the lens surface Repeat this procedure until the lens is completely clean Use a clean piece of lens tissue each time Caution when objective lens is contaminated If an objective lens is contaminated by unsuitable immersion oil or by the 1 specimen please contact your local Leica branch office Certain solvents may dissolve the glue which holds the lens in place Care Always keep the optical components of the microscope clean Never touch the optical components with your fingers or anything which may bear dust or grease Always place dust caps over the objective nosepiece positions when no objective is in place in the nosepiece
28. the steps e Acquire step Experiments tab Directory tree for opened files Acquisition tab Hardware settings for the current experiment and parameter settings for image acquisition Process step Experiments tab Directory tree for opened files Process Tools tab Overview of the functions available in this step e Quantify step Experiments tab Directory tree for opened files Tools tab Overview of the functions available in this step Working area Depending on the step and tab selected different dialogs are available in the working area e Acquire step Beam Path Settings In this dialog the beam path is shown schematically You can select and adjust the lasers and the system components along the beam path and the detectors for image acquisition e Process step Appropriate processing options and a preview Image appear in the working area based on which function you have selected in the Process Tools tab e Quantify step The working area is divided into two tabs Graphs Graphic representation of values that were measured in the entire image or in regions of interest ROI Statistics Display of statistical values that were determined in the entire image or in plotted regions of interest ROI Image display Acquired images can be displayed and regions of interest ROI and additional information can be plotted here Image display settings This area contains buttons used to change the display options for acquired image
29. this laser class When using an MP system you must wear laser safety glasses Order No 158002570 Appropriate laser safety glasses for IR laser radiation are provided with the system when delivered During the scanning operation all persons present in the room must wear such laser safety glasses These laser safety glasses do not provide any protection against visible laser radiation visible spectrum It is not necessary to wear protective eyewear when using VIS and UV lasers When used as intended and safety notes have been followed laser radiation is kept within the limit value that eliminates the chance for eye injuries Never look directly into a laser beam or a reflection of the laser beam Avoid all contact with the laser beam Never expose your eyes or skin to direct or indirect laser radiation The radiation can cause irreparable eye and skin injuries During the scanning operation the laser radiation is accessible in the microscope s specimen area without obstruction after coming out of the objective Always maintain a 5 5 nominal ocular hazard distance of at least 20 8 between your eyes and the opening of the objective Figure 1 Specimen area of upright and inverted microscope Make sure that the fiber optic cables are not damaged The system may not be turned on with damaged fiber optic cables as laser radiation can escape and lead to irreparable eye and skin injuries It is not necessary to wear
30. 10 1 2010 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements EN 60825 1 2007 Safety of laser products Part 1 Equipment classification and requirements For the microscope models DM and DMI the respective EC Declaration of Conformity with its corresponding EC Directives is valid This declaration shall cease to be valid if modifications are made to the product without our approval Mannheim Germany i V Laabs BE May 30 2012 Head of RA QA Figure 121 Declaration of Conformity 138 24 4 People s Republic of China Administrative Measures on the Control of Pollution Caused by Electronic Information Products SS Si Hazardous substances fa TREE SS RAR Pb HD CD rf PBB PBLE X printed circuit boards X 9 A 9 mechanical parts adea optical components Str FF Name of the part IN X X O light sources o 5 11363 2006 MEA EM EE m GER DI T Indicates that the concentration of the hazardous substance in all materials in the parts is below the relevant threshold of the SJ T 11363 2006 standard x RAIBABRSORESEZATHR HEP HSS BMS 11363 2006 S EX B SEX Indicates that the concentration of the hazardous substance of at least one of all materials in the parts is above the relevant threshold of the SJ T 11363
31. 3 Switch on the workstation and the microscope on the main switch board of the flexible supply unit see Figure 57 item 1 You do not have to start the operating system it starts automatically when you switch on the workstation Wait until the startup process has concluded Scanner Laser Laser Microscope Power Power Emission Figure 57 Overview of the main switch board on the flexible supply unit Check whether the microscope is switched on The microscope is operational once the readiness indicator Figure 58 item 1 on the toggle switch is lit up If the readiness indicator is not lit activate the electronics box toggle switch Figure 58 item 2 Figure 58 Microscope electronics box 7 Switch on the microscope s control panel Figure 59 Control panel for the microscope 8 If your system has an infrared laser MP or an optical parametric oscillator OPO switch on the NDD detection unit Figure 60 Switch on the NDD detection unit 9 Switch on the scan head on the flexible supply unit s main switch board see Figure 57 item 2 10 Switch on the lasers on the main switch board of the flexible supply unit see Figure 57 item 3 The power supplies and fan of the system have now been started 11 If your system has an infrared laser MP or an optical parametric oscillator OPO switch on these components as described in the manufacturers respective user 03 manual 12 I
32. 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEOFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN MP DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 4 LASERSTRAHLUNG KLASSE 4 WHEN OPEN AVOID EYE OR SKIN WENN ABDECKUNG GE FFNET EXPOSURE TO DIRECT OR BESTRAHLUNG VON AUGE ODER SCATTERED RADIATION HAUT DURCH DIREKTE ODER STREUSTRAHLUNG VERMEIDEN Table 1 Safety labels for the inverted microscope see Figure 43 Front view of the microscope Figure 44 Safety labels for the inverted microscope Position System Safety label in English Safety label in German VIS LASER RADIATION IS EMITTED AUSTRITT VON FROM THIS APERTURE LASERSTRAHLUNG AVOID EXPOSURE BESTRAHLUNG VERMEIDEN UV MP VISIBLE AND INVISIBLE LASER AUSTRITT VON SICHTBARER RADIATION IS EMITTED FROM UND UNSICHTBARER THIS APERTURE LASERSTRAHLUNG AVOID EXPOSURE BESTRAHLUNG VERMEIDEN Table2 Safety labels for the inverted microscope see Figure 44 69 11 2 Upright Microscope Rear view of microscope O Figure 45 Safety labels for the upright microscope Position System Safety label in English Safety label in German STREUSTRAHLUNG VERMEIDEN VIS DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEOFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN UV DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE
33. 60 non condensing Maximum location elevation 2000 m above sea level HyD RLDs are cooled To ensure optimal performance from the detectors the humidity of the surrounding air must not exceed 60 at 25 C To prevent potential damage due to condensation the cooling shuts off automatically if tts value is exceeded Vibrations Vibrations must be reduced to a minimum for that reason do not set up the system near any of the following sources of vibration shakers ultracentrifuges pumps compressors refrigerators vending machines elevators Maximum tolerable vibrations Frequency range 5 Hz 30 Hz less than 30 um s root mean square Frequency range gt 30 Hz less than 60 um s root mean square 43 44 9 3 Room Dimensions The room should be as large as possible so that multiple operators can find room around the system The free space to the right left and rear of the system should be 60 cm In front of the system there must be 110 cm of free space so that the service technicians can access the system as necessary WARNING Fire or overheating of the system possible Electrical peripheral devices must be placed at least 10 cm 4 away A from the wall and from flammable substances to prevent overheating or fire of the system Also make sure that doors and passages are at least 100 cm wide in order to be able to safely transport the system The following minimum size should be planned for the space Minimu
34. 7 Figure 88 Piezo focus controller Spacer on objective 17 Switching Off the System The TCS SP8 and TCS SP8 MP systems are available with a flexible or compact supply unit Observe the switch off sequence that applies to your supply unit NOTICE Damage to the instrument when not adhering to the switch off sequence The switch off sequence must be followed When not adhering to the below listed switch off sequence the laser can be damaged 17 1 TCS SP8 SP8 X with Flexible Supply Unit Observe the user manuals for external lasers BA Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 1 Save the image data do so click on the Experiments tab and click the Save all button Experiments Acquisition i Expenment b p Expenment New Open Save Al Apply Browse A Use Default Image Base Name Figure 89 Saving the image data in LAS AF 107 2 You can shift the infrared laser e g InSight DeepSee into a type of sleep mode without switching off the supply unit and cooling for the IR laser This allows hours of acclimatization to be avoided later To do so click on Configuration gt Laser Config MP Laser in LAS AF and deactivate the infrared laser in the Currently Available Lasers window Switch on the supply unit and cooling for the laser If you have no need for the InSight DeepSee
35. 77 579 6 678 443 6 687 035 6 738 190 6 754 003 6 771 405 6 801 359 6 831 780 6 850 358 6 852 964 6 867 899 7 016 101 Further patents are pending The Leica TCS SP8 X product is protected by US patents 5 886 784 5 903 688 6 137 627 6 222 961 6 285 019 6 311 574 6 355 919 6 423 960 6 433 814 6 444 971 6 466 381 6 510 001 6 611 643 6 614 031 6 614 525 6 614 526 6 654 165 6 657 187 6 677 579 6 678 443 6 687 035 6 710 918 6 738 190 6 754 003 6 771 405 6 796 699 6 801 359 6 831 780 6 850 358 6 852 964 6 867 899 6 888 674 6 898 367 6 958 858 7 016 101 7 110 645 7 123 408 7 257 289 7 679 822 Further patents are pending Safety Data Sheets from Third Party Manufacturers The scan head is liquid cooled Following are the safety data sheets from the manufacturer Innovatek for the coolant used 131 EEC SAFETY DATA SHEET Gem 91 155 EG 2001 58 EG Dated February 28st 2011 innovatek Protect application mixture innovatek TE gt innovatek OS GmbH www innovatek de info innovatek de 1 Substance preparation and company name Trade name company Tel 08405 92590 Fax 08405 925921 innovatekProtect IP application mixture innovatek OS GmbH Stadtweg 9 85134 Stammham Emergency phone No 49 0 8405 92590 2 Composition information on ingredients Chemical nature Hazardous Compounds Ethanediol 2 ethylhexanoic acid sodium salt Ethylene glycol etha
36. BARE UND UNSICHTBARE LASERSTRAHLUNG KLASSE 3B WENN ABDECKUNG GEOFFNET NICHT DEM STRAHL AUSSETZEN VORSICHT SICHTBARE UND UNSICHTBARE LASERSTRAHLUNG KLASSE 4 WENN ABDECKUNG GEOFFNET BESTRAHLUNG VON AUGE ODER HAUT DURCH DIREKTE ODER STREUSTRAHLUNG VERMEIDEN 71 78 11 9 11 10 White light laser Rear side of white light laser NY O25020 RORORO lee Se OSOS eO Q5 Figure 53 Safety label on the rear side of the white light laser DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GE FFNET NICHT BEAM DEM STRAHL AUSSETZEN LASER RADIATION LASERSTRAHLUNG AVOID DIRECT EXPOSURE TO BEAM NICHT DEM STRAHL AUSSETZEN 500mW 400 700nm 500mW 400 700nm CLASS 3B LASER PRODUCT LASER KLASSE 3B 60825 1 2007 IEC 60825 1 2007 Table 11 Safety Label on the Rear Side of the White Light Laser see Figure 53 External UV Laser Figure 54 Safety Label on External UV Laser 405 11 11 Other External Lasers Observe the user manuals for external lasers BA Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 79 Safety Labels on the System 80 12 Switching On the System The TCS SP8 and TCS SP8 MP systems are available with a flexible or compact supply unit Observe the switch on sequence that
37. DANGER VISIBLE AND INVISIBLE LASER RADIATION CLASS 3B WHEN OPEN AVOID EXPOSURE TO BEAM DANGER VISIBLE AND INVISIBLE LASER RADIATION CLASS 4 WHEN OPEN AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION Safety Labels on the Cover see Figure 51 VORSICHT LASERSTRAHLUNG KLASSE 3B WENN ABDECKUNG GEOFFNET NICHT DEM STRAHL AUSSETZEN VORSICHT SICHTBARE UND UNSICHTBARE LASERSTRAHLUNG KLASSE 3B WENN ABDECKUNG GEOFFNET NICHT DEM STRAHL AUSSETZEN VORSICHT SICHTBARE UND UNSICHTBARE LASERSTRAHLUNG KLASSE 4 WENN ABDECKUNG GEOFFNET BESTRAHLUNG VON AUGE ODER HAUT DURCH DIREKTE ODER STREUSTRAHLUNG VERMEIDEN lf the replacement flange for transmitted light is not equipped with a functional module such as a lamp housing a cover must be placed over the opening for laser safety reasons 11 8 Mirror Housing Figure 52 Position System Safety label in English Safety label in German VIS UV Table 10 Safety label on the mirror housing top DANGER LASER RADIATION CLASS 3B WHEN OPEN AVOID EXPOSURE TO BEAM DANGER VISIBLE AND INVISIBLE LASER RADIATION CLASS 3B WHEN OPEN AVOID EXPOSURE TO BEAM DANGER VISIBLE AND INVISIBLE LASER RADIATION CLASS 4 WHEN OPEN AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION Safety label on the mirror housing see Figure 52 VORSICHT LASERSTRAHLUNG KLASSE 3B WENN ABDECKUNG GEOFFNET NICHT DEM STRAHL AUSSETZEN VORSICHT SICHT
38. Figure 38 ajo Figure 38 Interlock Connector on the White Light Laser Interlock connector when using the white light laser If the white light laser is operated as a component of the TCS SP8 X system you have to use the interlock connector on the supply unit The shorting plug must be connected the interlock connector of the white light laser If you operate the white light laser separately without connecting it to the TCS SP8 system you have to use the interlock connector on the white light laser for connecting remote interlocks Remote interlock devices such as those connected to the room the door or other onsite safety interlock systems can also be connected to the remote interlock connector The laser beam path is interrupted if the contact is open 62 10 4 3 10 4 4 Interlock Connector on Other External Lasers Observe the user manuals for external lasers BA Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes Interlock Connector on the Scan Head The interlock connector is located on the rear side of the scan head operating voltage 12 V DC see Figure 39 For laser safety reasons the inverted microscope must be connected to this connection or If an upright microscope is used to the mirror housing This ensures that the
39. In the menu bar click on the Help menu The menu drops down and reveals search related options including the following Help Contents Index Search About Figure 84 Online help menu Contents Opens the table of contents for Online Help Index Opens the index for Online Help Search Opens the full text search function for Online Help About Calls up the User Configuration dialog 97 98 13 3 3 13 3 4 Selecting the Language for Online Help By default English is set as the language for Online Help In order to select another language follow these steps In LAS AF switch to the Configuration step Click on the User Config button on the left side to access the User Configuration dialog In the User Configuration dialog select the desired language under Help Language Configuration Ta Copyright 1997 2012 Leica Microsystems CMS GmbH Y User Path Help Language System defined default User defined Codemeter Web Administration Tr USB Dnve Check Licences Excel Export Options CSV LUT Mode Figure 85 Selecting the language for Online Help The next time you call up Online Help the help topics will be displayed in the selected language The Help menu provides a second option for changing the language Call up the Help gt About menu The User Configuration dialog opens and you can select the desired language see Figure 85 Using Online Help
40. LUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEOFFNET NICHT BEAM DEM STRAHL AUSSETZEN Table 6 Safety Labels on the Compact Supply Unit see Figure 48 Flexible Supply Unit View of the flexible supply unit Figure 49 Safety labels on the flexible supply unit Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE TO WENN ABDECKUNG GEOFFNET NICHT BEAM DEM STRAHL AUSSETZEN Table 7 Safety Labels on the Flexible Supply Unit see Figure 49 11 6 MP Beam Coupling Unit Angled front view of the right side of the MP beam coupling unit A Figure 50 Safety label for the MP beam coupling unit top side Position Safety label in English Safety label in German DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 4 LASERSTRAHLUNG KLASSE 4 WHEN OPEN AVOID EYE OR SKIN WENN ABDECKUNG GEOFFNET EXPOSURE TO DIRECT OR SCATTERED BESTRAHLUNG VON AUGE ODER HAUT RADIATION DURCH DIREKTE ODER STREUSTRAHLUNG VERMEIDEN Table8 Safety label for the MP beam coupling unit top see Figure 50 15 16 11 7 Cover for replacement flange Front view of the cover Figure 51 Safety labels on the cover Position System Safety label in English Safety label in German VIS UV Table 9 DANGER LASER RADIATION CLASS 3B WHEN OPEN AVOID EXPOSURE TO BEAM
41. P8 X with inverted microscope Inverted microscope Control panel SmartMove Fluorescence lamp EL6000 Workstation Microscope electronics box Scan head 0 White light laser Main switch board nF c9 N co co QC Flexible supply unit Figure 19 Dimensions of TCS SP8 X with inverted microscope 37 7 4 TCS SP8 with External UV laser 110 cm 22cm cm 20 cm 20cm 80cm 280 02053 cm 230 253 cm NM T 1 TT 8 4 362 385 cm 11 11 12 6 Figure 20 Overview with dimensions of TCS SP8 with external UV laser 1 Workstation 4 Power supply external UV laser 2 Stage with supply unit 5 External UV laser 3 Microscope stage with scan head 7 5 Controls on the Supply Unit 7 5 1 Main Switch Board on the Flexible Supply Unit BO om Scanner Laser Laser PE Power Power Emission Figure 21 Overview of the main switch board on the flexible supply unit 38 7 5 2 Control Panel Field on the Compact Supply Unit Scanner Laser Laser Power Power Emission Figure 22 Overview of the Control Panel Field on the Compact Supply Unit 39 40 1 6 7 6 1 7 6 2 Technical Data Dimensions Dimensions of the system length x depth x height TCS SP8 with upright microscope 230 x 110 x 175 cm 77 x 3 7 x 5 9 TCS SP8 with inverted microscope 253 x 110 x 140 cm 84 x 3 7 x 4 7 TCS SP8 MP with DM6000 and IR Laser See Figure 10 wi
42. R PRODUCT IEC 60825 1 2007 LASER RADIATION VISIBLE AND INVISIBLE CLASS 3B AVOID DIRECT EXPOSURE TO BEAM 500mW 350 700nm IEC 60825 1 2007 LASER RADIATION VISIBLE AND INVISIBLE CLASS 4 AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION P 4W 350 1600 gt 80fs IEC 60825 1 2007 Safety label in English Safety label in German VORSICHT LASERSTRAHLUNG KLASSE 3B WENN ABDECKUNG GEOFFNET NICHT DEM STRAHL AUSSETZEN VORSICHT SICHTBARE UND UNSICHTBARE LASERSTRAHLUNG KLASSE 3B WENN ABDECKUNG GEOFFNET NICHT DEM STRAHL AUSSETZEN VORSICHT SICHTBARE UND UNSICHTBARE LASERSTRAHLUNG KLASSE 4 WENN ABDECKUNG GEOFFNET BESTRAHLUNG VON AUGE ODER HAUT DURCH DIREKTE ODER STREUSTRAHLUNG VERMEIDEN LASERSTRAHLUNG NICHT DEM STRAHL AUSSETZEN lt 500mW 400 700nm LASER KLASSE 3B IEC 60825 1 2007 LASERSTRAHLUNG SICHTBAR UND UNSICHTBAR KLASSE 3B NICHT DEM STRAHL AUSSETZEN 500mW 350 700nm IEC 60825 1 2007 LASERSTRAHLUNG SICHTBAR UND UNSICHTBAR KLASSE 4 BESTRAHLUNG VON AUGE ODER HAUT DURCH DIREKTE ODER STREUSTRAHLUNG VERMEIDEN P 4W 350 1600nm gt 80fs IEC 60825 1 2007 Table 5 Safety Labels on the Scan Head see Figure 47 74 11 4 11 5 Compact Supply Unit View of the compact supply unit Figure 48 Safety labels on the compact supply unit Position Safety label in English Safety label in German DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAH
43. RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEOFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN MP DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 4 LASERSTRAHLUNG KLASSE 4 WHEN OPEN AVOID EYE OR SKIN WENN ABDECKUNG GEOFFNET EXPOSURE TO DIRECT OR BESTRAHLUNG VON AUGE ODER SCATTERED RADIATION HAUT DURCH DIREKTE ODER Table3 Safety labels for the upright microscope see Figure 45 70 Front view of the microscope Tem Figure 46 Safety labels for the upright microscope LN Safety label in English Safety label in German LASER RADIATION IS EMITTED AUSTRITT VON FROM THIS APERTURE LASERSTRAHLUNG AVOID EXPOSURE BESTRAHLUNG VERMEIDEN and2 UV MP VISIBLE AND INVISIBLE LASER AUSTRITT VON SICHTBARER RADIATION IS EMITTED FROM UND UNSICHTBARER THIS APERTURE LASERSTRAHLUNG AVOID EXPOSURE BESTRAHLUNG VERMEIDEN Table 4 Safety labels for the upright microscope see Figure 46 71 Scan Head 11 3 Front view of the scan head Safety labels on the scan head Figure 47 72 DANGER LASER RADIATION CLASS 3B WHEN OPEN AVOID EXPOSURE TO BEAM DANGER VISIBLE AND INVISIBLE LASER RADIATION CLASS 3B WHEN OPEN AVOID EXPOSURE TO BEAM DANGER VISIBLE AND INVISIBLE LASER RADIATION CLASS 4 WHEN OPEN AVOID EYE OR SKIN EXPOSURE TO DIRECT OR SCATTERED RADIATION LASER RADIATION AVOID DIRECT EXPOSURE TO BEAM 500mW 400 700nm CLASS 3B LASE
44. a dry place Storage in galvanized containers is not recommended 8 Exposure controls and personal protection Components with workplace control parameters 107 21 1 Ethylene glycol MAK value D 26 mg m 10 ppm TRGS 900 DE Top limit category 1 There is no reason to fear a risk of damage to the developing embryo when the MAK value is adhered to Skin resorption hazard wear suitable gloves see below Personal protective equipment Respiratory Protection Do not inhale gases vapours aerosols Hand protection Chemical resistant protective gloves EN 374 Recommended nitrile rubber protective index 6 Manufacturers directions for use should be observed because of great diversity of types Eye protection Safety glasses with side shields frame goggles EN 166 General safety and The usual precautions for the handling of chemicals must hygiene measures be observed 9 Physical and chemical properties Form Liquid Colour Colourless Odour Product specific pH value 500 g l 20 C 7 0 9 0 Solidification temperature lt 10 C DIN ISO 3016 Boiling point range gt 105 C ASTM D 1120 Flash point gt 120 C only valid fur the Ethylenglykol part DIN ISO 2592 Lower explosion limit 3 0 vol only valid fur the Ethylenglykol part 2 Figure 117 innovatek Protect application mixture safety data sheet page 2 133 134 SAFETY DATA SHEET Gem 91 155 EG 2001 58 EG n ovatek TE gt
45. a service personnel may open or work on the supply unit and the scan head 121 Repairs and Service Work 122 20 20 1 Maintenance NOTICE Damage to the instrument from not paying attention to the maintenance Absolutely adhere to the prescribed maintenance intervals as otherwise there can be serious damage to the instrument Having Coolant Replaced The coolant such as for scan head cooling and cooling the HyD RLD must be replaced by Leica Service every two years You can find the safety data sheet for the coolant in the Chapter Appendix Maintenance and replacement of the coolant may only be carried out by service technicians who are authorized by Leica Microsystems CMS GmbH Opening or working on the system in any way shall void any and all warranty claims WARNING Contamination with hazardous substances Before each call from a service technician the system has to be cleaned thoroughly to avoid contamination with hazardous biological materials A When returning system parts it must be ensured that they are free of hazardous substances otherwise they must not be sent This applies in particular to systems that are located in biomedical research labs 123 124 21 Disassembly and Transport Do not disassemble system components or housing parts yourself Opening or working on the system in any way shall void any and all warranty claims Contact the Leica branch office in your country or your contact person
46. age with supply unit compact or flexible 4 Beam coupling unit 5 IR laser without dispersion compensation 2 Optical table 90 x 120 cm 2 11 x 3 11 6 Power supply and cooling of the IR laser 3 Microscope DM6000 7 2 2 TCS SP8 MP with DM6000 and IR Laser with Dispersion Compensation 130 cm 4 3 180 cm 5 11 500 cm 16 5 Figure 11 System overview of the TCS SP8 MP with DM6000 and IR laser with dispersion compensation 1 Stage with supply unit compact or flexible 4 Beam coupling unit 5 IR laser with dispersion compensation 2 Optical table 180 x 120 cm 5 11 x 3 11 6 Power supply and cooling of the IR laser 3 Microscope DM6000 31 32 1 2 3 TCS SP8 MP with DMI6000 and IR Laser without Dispersion Compensation 130 cm 4 3 90 cm 2 11 370 cm 122 Figure 12 System overview of the TCS SP8 MP with DMI6000 and IR laser without dispersion compensation 1 Stage with supply unit compact or flexible Microscope DMI6000 Beam coupling unit 2 Power supply and cooling of the IR laser IR laser without dispersion compensation Optical table 90 x 150 cm 211 x 4 11 cO c A 7 2 4 TCS SP8 MP with DMI6000 and IR Laser with Dispersion Compensation 130 cm 4 3 180 cm 5 11 500 cm 16 5 Figure 13 System overview of the TCS SP8 MP with DMI6000 and IR laser with dispersion compensation 1 Stage with supply unit co
47. applies to your supply unit NOTICE NOTICE Damage is possible when contacting the specimen stage When using an inverted microscope the illuminator arm must be swung back before the system start and LAS AF start because the stage can damage the condenser the objective or the specimen during initialization Objective damage is possible when contacting the specimen stage When using an upright microscope the specimen stage must be moved down before the system start and LAS AF start because it could come into contact with the objective during initialization and damage the objective 121 5 SP8 SP8 X with Flexible Supply Unit an Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 1 Switch on the cooling for the infrared laser see Figure 55 item 1 The cooling requires approx 4 hours to regulate the temperature of the infrared laser 2 After 4 hours switch on the supply unit for the infrared laser see Figure 55 item 2 Figure 55 Cooling and supply unit of the InSight DeepSee infrared laser 81 02 3 If you are using a fluorescence lamp EL6000 switch it on first Figure 56 EL6000 If you are using a HyD RLD switch it on at its supply unit see Figure 78 You can find additional information on the HyD RLD in Chapter 12
48. are Additional information about specific functions can be found in the online help The instructions contained in this documentation reflect state of the art technology and knowledge standards at the time of publication Leica Microsystems CMS GmbH reserves the right to revise this documentation and or to further develop and improve the products described in this document at any time without prior notice or any other obligation If you have any suggestions or improvements for this User Manual please contact the Leica branch office in your country 11 About this User Manual Intended Use This system is intended for use in a lab The system was designed for confocal scanning laser scanning images of fluorescence marked living and fixed specimens as well as for quantitative measurements in the area of life science Applications of in vitro diagnostics in accordance with MPG German Medical Devices Act are excluded from proper intended use This system must not be used together with life support systems such as those found in intensive care wards The owner operator and user of this product are responsible for proper and safe operation and safe maintenance of the system and for following all applicable safety regulations The owner operator and user are fully liable for all consequences resulting from the use of the system for any purposes other than those listed in the User Manual or the online help The manufacturer assumes no liab
49. ate of the firmware a continuous tone sounds After the updated component is automatically restarted the signal stops During the automatic update and the automatic restart of the component you may not switch off or restart the system since otherwise this can lead to damage to the system Protect the system from dust and grease Make sure to use only one small drop of immersion fluid The immersion fluid may not contaminate or enter the microscope Make sure that the specimen carrier is not againstthe objective and cannot be damaged by it or cause broken glass Be absolutely certain to preventthe optics and mechanical parts from coming into direct contact with acids bases and other aggressive chemicals Never use abrasive products to clean the system and its components Abrasives can scratch the surface and thus have a negative effect on the protection of the parts Protect the microscope from excessive temperature fluctuations Such fluctuations can lead to the accumulation of condensation which can damage the electrical and optical components Allow the entire system to cool down to room temperature before covering the system with a dust cover This prevents condensation from forming below it which can enter the system and damage it When used as intended the HyD reflected light detectors are sufficiently protected from destruction due to overexposure by measures in LAS AF and by an electronic protective circuit An audible signa
50. ater must be disposed of in accordance with local legislation innovatek Protect application mixture safety data sheet page 1 SAFETY DATA SHEET Gem 91 155 EG 2001 58 EG n ovatek innovatek OS GmbH Dated www innovatek de February 28st 2011 innovatek Protect application mixture info innovatek de 6 Accidental release measures Personal precautions Avoid excessive contact with skin and eyes In case of release of larger amounts remove contaminated clothing and wash bo dy down thoroughly with water Hand protection Pick up im mediately as product renders floors slippery Environmental pre Contain contaminated water firefighting water Do not discharge cautions product into natural waters without pretreatment biological treatment plant Methods for cleaning Bind the liquid by using suitable absorbent material saw dust up taking up sand etc and dispose of in accordance with the regulations Wash away spills thoroughly with large quantities of water In case of release of larger quantities which might flow into the draining system or waters contact appropriate authorities 7 Handling and storage Handling Ensure thorough ventilation of stores and work areas Protection against Take precautionary measures against static discharges fire and explosion If exposed to fire keep containers cool by spraying with water Storage Product is hygroscopic Containers should be stored tightly sealed in
51. crosystems CMS GmbH shall not be liable for any injury or property damage caused by untrained or unauthorized persons Important Information for Operators and Users e The owner operator is required to designate a Laser Safety Officer or a Laser Protection Advisor according to the applicable legal requirements in each country e he owner operator and user of this product are responsible for proper and safe operation and safe maintenance of the system and for following all applicable safety regulations e The owner operator and user are fully liable for all consequences resulting from the use of the system for any purposes other than those listed in the User Manual or the online help e he owner operator and user are obligated to perform and monitor suitable safety measures according to national regulations e The owner operator and user are responsible for observing the laser safety regulations according to applicable country specific regulations e he owner operator and user must ensure that this laser product is commissioned and operated only by persons who have been trained in the use of the system and the potential dangers of laser radiation e The owner operator and user are fully liable for all consequences resulting from the use of the system if it is opened improperly serviced or repaired by persons other than authorized Leica service representatives 15 Liability and Warranty 4 Meaning of the warning messages in th
52. ct Supply Unit The emission warning indicator goes out 6 If you are using a RLD switch it off at its supply unit Figure 108 HyD RLD supply unit 7 If your system has an infrared laser MP or an optical parametric oscillator switch off the NDD detection unit 115 116 Figure 109 NDD detection unit 0 Switch off the microscope s control panel Figure 110 Control panel for the microscope 9 Now shut down the workstation 10 Switch off the lasers see Figure 107 item 2 and the scan head see Figure 107 item 1 on the front side of the compact supply unit 11 Turn off the microscope by actuating the electronics box toggle switch Figure 111 item 2 The readiness indicator Figure 111 item 1 on the electronics box goes out Figure 111 Microscope electronics box 12 Turn off any accessories being used 13 If you are using external 355 nm UV laser including 405 nm wavelength activate the following key switch to turn it off The emission warning indicators go out Figure 112 Key switch on the power supply of external 355 nm UV laser Figure 113 Key switch on the external 405 nm UV laser 14 Switch off both external 355 nm and 405 nm UV laser main power switches 117 118 Figur
53. detector was shut off Flashes if photons are being detected e Never change or remove the filter cube or short pass filter during operation e The filter cube cover see Figure 79 item 2 ensures the housing s EMC stability and prevents light from entering or exiting Replacement of the short pass filter is only allowed starting from LAS AF Version 2 7 or later 13 13 1 LAS AF The LAS AF software is used to control all system functions and acts as the link to the individual hardware components Image acquisition image analysis and image processing are carried out using LAS AF Starting LAS AF NOTICE Damage is possible when contacting the specimen stage When using an inverted microscope the illuminator arm must be swung back before the system start and LAS AF start because the stage can damage the condenser the objective or the specimen during initialization NOTICE Objective damage is possible when contacting the specimen stage When using an upright microscope the specimen stage must be moved down before the system start and LAS AF start because it could come into contact with the objective during initialization and damage the objective 1 Click the LAS AF symbol on the desktop to start the software 2 Select whether the system is to be operated in resonant Resonant On or conventional mode Resonant Off Leica Application Suite MICROSYSTEMS Advanced Fluorescence Configuration Microscope Reso
54. e 114 Power switch on external 355 nm UV laser Figure 115 an Power switch on external 405 nm UV laser Observe the user manuals for external lasers Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 18 18 1 18 2 Care and Cleaning Observe the user manuals provided BA Always observe all of the user manuals provided for the individual components and peripheral devices WARNING _ Electric shock Before cleaning disconnect the entire system from the power supply To do so use the power switches of all components and disconnect all A power cables from the power supply Ensure that no fluids enter the individual components or peripheral devices during cleaning Cleaning Surfaces e Never use abrasives Abrasives can scratch the surface and thus have a negative effect on the protection of the parts e Remove dust and loose dirt particles using a soft brush or lint free cotton cloth e You may clean uncoated or plastic surfaces only using a dry cotton cloth or one moistened with a little water Other cleaning agents can attack and tarnish the surface and cause it to become porous e Carefully remove clinging dirt on coated surfaces using a clean cloth slightly moistened with water e Never use acetone xylene or nitro thinners as they attack the varnish Cleaning
55. e a high performance air conditioning system We recommend high performance air conditioning system for 1 operating the system to prevent large temperature fluctuations in the room TCS SP8 with flexible supply unit Waste heat of the complete system including the argon laser Waste heat of the argon laser For cooling the argon laser must be connected to the provided argon laser fan The following space is required Standard fan about 30 x 30 cm 1 x 1 Noise reduced laser fan 50 x 60 cm 1 8 x 2 In addition it is possible to connect the argon laser exhaust to an in house exhaust system Hose length 400 cm 13 1 provided by default Diameter of adapter piece 15 cm 6 Air flow rate 400 m3 h 14 126 ft3 h 9 6 2 8 6 3 8 6 4 The remaining waste heat 1 6 kW must be extracted otherwise the room temperature will rise TCS SP8 with compact supply unit Waste heat of the complete system with a compact supply unit TCS SP8 MP with flexible supply unit Waste heat of the complete 6 2 kW system including the argon laser HyD RLD 100 W Waste heat of the argon laser 1 6 kW For cooling the argon laser must be connected to the provided argon laser fan The following space is required Standard fan about 30 x 30 1 x 1 Noise reduced laser fan 50 x 60 cm 1 8 x 2 In addition it is possible to connect the argon laser exhaust to an in house exhaust system Hose length 400 c
56. e manual WARNING gt WARNING WARNING WARNING WARNING WARNING Electric shock This warns you of hazardous electrical voltage Following the instructions is mandatory since otherwise there Is a risk of severe or fatal injury Severe injuries from This note warns you of hazards that can cause severe or fatal injuries Permanent eye and skin damage from laser radiation This note warns you of eye and skin damage that can occur when using lasers if safety precautions are not taken Risk of injuries due to harmful or irritating substances This note warns you of substances that pose a health hazard Risk of injuries due to biological substances This note warns you of biological substances that pose a health hazard Risk of burns on hot surfaces This note warns you of hot surfaces that can cause burns 17 18 CAUTION NOTICE NOTICE DATA 1 Injuries from This note warns you of minor to moderate injuries that can be prevented by following instructions Risk of damage to the system This note describes possible material damage that can occur in case of misuse Loss of data This note warns you of the potential for losing data Observe user manual This mandatory sign indicates that an additional user manual must be followed Wear laser safety glasses This mandatory sign indicates that laser safety glasses must be worn to prevent eye injuries Add
57. er class 116 VIS and UV lasers or for laser class 4 IV MP systems in accordance with applicable national and federal regulations You may only use the laser listed here as the laser safety devices are only designed for the laser variants listed here Laser Classes System variant System variant Usable Wavelength range laser class lasers V 400 700 nm visible laser radiation SP8 3B IIIb GE LE 350 400 nm invisible laser radiation 400 700 nm visible laser radiation SP8X 3B IIIb 680 1600 nm fis asor radiation Overview of Usable Lasers VIS UV Lasers for TCS SP8 The TCS SP8 features a combination of the lasers listed below Laser type Wavelength Maximum Maximum Pulse duration light output at light output in laser output focal plane mW 355 442 448 91 52 9 2 2 9 2 3 9 2 3 1 OPSL 514 350 Continuous wave cw OPSL 552 552 350 Continuous wave cw DPSS 561 lt 100 12 Continuous wave cw HeNe 4 1 Continuous wave cw HeNe 633 15 lt 5 Continuous wave cw Diode 638 638 350 Continuous wave cw VIS UV Lasers for TCS SP8 X The TCS SP8 X features a combination of the lasers listed below Laser type Wavelength Maximum Maximum Pulse duration nm light output at light output in laser output focal plane mW mW Diode 405 lt 120 lt 7 Continuous wave cw Diode 405 p lt 5 mean lt 0 3 mean pulsed 60 ps power power
58. er supply voltage 100 V 120 V fuse protection 20 A Or Power supply voltage 200 V 240 V fuse protection 12 16 A Two for the flexible supply unit s power supply and one for supplying peripheral devices To prevent damage to the instrument the two types of voltage 200 to 240 V and 100 to 120 V must not be combined Depending on the system configuration further electrical circuits may be required Power supply for USA Two terminals of the NEMA 5 20 type for the supply unit Terminals of the NEMA 5 15 type for the remaining instruments When using HyD RLDs An additional power supply 8 4 4 TCS SP8 MP with compact supply unit Supply voltage 100 V to 240 V 10 grounded SIT Power connection Three separate electric circuits with Power supply voltage 100 V 120 V fuse protection 20 A or Power supply voltage 200 V 240 V fuse protection 12 16 A One for the compact supply unit s power supply and one for supplying peripheral devices To prevent damage to the instrument the two types of voltage 200 to 240 V and 100 to 120 V must not be combined Depending on the system configuration further electrical circuits may be required Power supply for USA Terminals of type NEMA 5 15 When using HyD RLDs An additional power supply 9 4 5 TCS SP8 X Supply voltage 100 V to 240 V 10 grounded 46 9 4 6 9 5 Power connection Power supply for USA External Lasers Three separate
59. er with the search terms The following logical operators are available e Excitation AND Wavelength Finds help topics that contain both the word excitation and the word wavelength e Excitation OR Wavelength Finds help topics that contain either the word excitation or the word wavelength or both e Excitation NEAR Wavelength Finds help topics where the word excitation and the word wavelength are located within a specific search radius This method also looks for words that are similar in spelling to the words specified in the phrase e Excitation NOT Wavelength Finds help topics that contain the word excitation and not the word wavelength 99 100 14 14 1 142 Changing the Specimen WARNING Risk of permanent eye damage from laser radiation Never change specimens during the scanning operation because laser radiation can escape uncontrolled from the specimen area Changing the Specimen on an Upright Microscope To change specimens on an upright microscope proceed as follows 1 Finish the scanning operation 2 Ensure that no laser radiation exists in the specimen area 3 Replace the specimen Insert the specimen correctly into the specimen holder Changing the Specimen on an Inverted Microscope To change specimens on an inverted microscope proceed as follows 1 Finish the scanning operation Ensure that no laser radiation exists in the specimen area Tilt the tra
60. f your system has a white light laser check whether the white light laser s power supply Is switched on The white light laser s power supply is switched on if the power switch on the rear side of the white light laser is at QoGeQoQoe RORERORO Figure 61 Power switch on the rear side of the white light laser 13 To switch on the laser in the supply unit activate the key switch on the main switch board of the flexible supply unit see Figure 57 item 4 WARNING Risk of permanent eye and skin damage from laser radiation From this time on laser radiation may be present in the specimen area of the laser scanning microscope Make sure to follow the safety notes for operation of the system 14 To switch on the white light laser activate the key switch on the front side of the white light laser _ D o QUUD Figure 62 Key switch for the white light laser 04 White light laser error messages An error report appears on the display of the white light laser Q 1 e ftheroomtemperature exceeds 40 C gt the white light laser switches Itself off and can only be turned back on after the room cools off n case of mechanical shock or vibrations gt switch off the white light laser and turn it back on after 10 seconds 15 If you are using external 355 nm UV laser including 405 nm wavelength make sure that the power switch is in the position
61. fer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 1 Save the image data Select the Experiments tab and click the Save all button Experiments Acquisition Expenment b Expenment New Open Save All Apply Browse A Use Default Image Base Name Figure 103 Saving the image data in LAS AF 2 You can shift the infrared laser e g InSight DeepSee into a type of sleep mode without switching off the supply unit and cooling for the IR laser This allows hours of acclimatization to be avoided later To do so click on Configuration gt Laser Config MP Laser in LAS AF and deactivate the infrared laser in the Currently Available Lasers window Switch on the supply unit and cooling for the laser If you have no need for the InSight DeepSee infrared laser for a longer period of time you can shut it off completely To shut the InSight DeepSee off completely call up the MP dialog see Figure 104 item 1 and then click on the button Laser shutdown Figure 104 item 2 In this case you have to deactivate the supply unit for the infrared laser Figure 105 item 1 as well After 15 minutes you can also switch off the cooling for the infrared laser Figure 105 item 2 113 o v Current Laser Values Wavelength 1150 nm Output Power 124W 5 Configuration Acquire Process v Adjust Laser 680 nm 1300
62. ge to the central nervous system Additional information The statements are based on the properties of the individual components There is no reason to fear a risk of damage to the developing embryo or fetus when the MAK value is ad hered to The whole of the information available provides no indication of a carcinogenic effect 12 Ecological information Ecotoxicity Toxicity to fish Leuciscus idus LC50 96 h gt 400 mg l Aquatic invertebrates daphnia magna EC50 48 h gt 400 mg l Aquatic plants algae EC50 72 h gt 400 mg l Microorganisms Effect on activated sludge Inhibition of de gradation activity in activated sludge is not to be anticipated during correct introduction of low concentrations Persistence and Elimination information Degradability Test method OECD 301A new version Method of analysis DOC reduction Degree of elimination gt 70 Evaluation readily biodegradable Additional information Other ecotoxicological advice Do not release untreated into na Figure 118 innovatek Protect application mixture safety data sheet page 3 SAFETY DATA SHEET Gem 91 155 EG 2001 58 EG n ovatek innovatek OS GmbH Dated www innovatek de February 28st 2011 innovatek Protect application mixture info innovatek de tural waters The product has not been tested The statement was derived from the properties of the individual components 13 Disposal considerations innovatekProtect must be dumped
63. he beam is passed through a safety beam guide and if necessary also passed through a fiber optic cable see Figure 42 This completely shields the laser beam until itleaves the microscope objective and reaches the specimen Figure 42 Safety beam guide 1 and IR laser 2 11 Safety Labels on the System The corresponding safety labels depend on the laser configuration VIS UV MP they are attached in either English or German at the following locations WARNING A Permanent eye and skin damage from failure to observe the safety labels on the system Make sure that the safety labels shown in the User Manual are attached to the system Safety labels may not be removed Missing or damaged safety labels must be attached immediately and at the described location Operation without the safety labels shown Is not permitted If you have any further questions contact your laser safety officer or the Leica branch office in your country immediately 67 68 11 1 Inverted microscope Rear view of microscope Figure 43 Safety labels for the inverted microscope Position System Safety label in English Safety label in German VIS DANGER VORSICHT LASER RADIATION CLASS 3B LASERSTRAHLUNG KLASSE 3B WHEN OPEN AVOID EXPOSURE WENN ABDECKUNG GEOFFNET TO BEAM NICHT DEM STRAHL AUSSETZEN UV DANGER VORSICHT VISIBLE AND INVISIBLE LASER SICHTBARE UND UNSICHTBARE RADIATION CLASS 3B LASERSTRAHLUNG KLASSE
64. ility for damage caused by or any risks arising from use of the microscopes for purposes other than those for which they are intended or not using the microscopes within the specifications of Leica Microsystems CMS GmbH In such cases the Declaration of Conformity shall be invalid 13 Intended Use 3 1 Liability and Warranty Leica Microsystems CMS GmbH shall not be liable for damages resulting from failure to observe the information in this User Manual The information here does not in any way modify the warranty and liability clauses contained in the general terms and conditions of Leica Microsystems CMS GmbH Repairs and servicing may be performed only by service technicians authorized by Leica Microsystems CMS GmbH Opening or working on the system in any way shall void any and all warranty claims The manufacturer assumes no liability for damage caused by or any risks arising from use of the microscopes for purposes other than those for which they are intended or not using the microscopes within the specifications of Leica Microsystems CMS GmbH In such cases the Declaration of Conformity shall be invalid Leica Microsystems CMS GmbH shall not be liable for any damage caused by incorrect storage improper transport or an unsuitable installation location Figures are for illustration purposes The system you purchased can deviate from the illustrations without Leica Microsystems CMS GmbH explicitly specifying such Leica Mi
65. information for using the system the necessary ambient conditions and the usable lasers It explains system startup The system Is assembled and disassembled by service technicians that have been authorized by Leica Microsystems CMS GmbH This is why unpacking assembly and installation of the system are not described in this manual You can find an overview of the system and specifications in the Chapter System Overview and Properties For information about special configurations such as optional lasers or specific objectives refer to the respective included manual This User Manual does not contain any information about basic optical principles or the operating principle of microscopes confocal systems and the like If you are interested in these topics or certain applications from the area of optics and confocal microscopy you can read more about them at the Leica Microsystems CMS GmbH knowledge portal http www leica microsystems com science lab The system is delivered with the latest version of the licensed Leica Application Suite Advanced Fluorescence LAS AF software In order to keep information up to date a description of software functions has been intentionally omitted from this user manual You can find up to date explanations and instructions for corresponding software functions in the LAS AF Online Help Read Chapter LAS AF in this User Manual in order to familiarize yourself with the design and basic operation of the softw
66. ing message is shown on the monitor within the LAS AF For safety reasons the shutters remain closed No further use of the laser is possible In this situation the system must not be operated Contact the Leica branch office in your country or your contact person 10 7 10 7 1 Audible warning signal in the case of defective parts in the shutter safety circuit f a part in the shutter safety circuit is defective a tone sounds at intervals For safety reasons the shutters remain closed No further use of the laser is possible In this situation the system must not be operated Contact the Leica branch office in your country or your contact person Audible warning signal in case of defective laser emission indicator port The relay contact for the laser emission indicator port is safety monitored If the relay fails an audible warning signal sounds at regular intervals Notify Leica Service immediately If a laser emission indicator is connected to the laser emission indicator port the system may not be operated Audible warning signal during automatic firmware update During the update of the firmware a continuous tone sounds After the updated component is automatically restarted the signal stops During the automatic update and the automatic restart of the component you may not switch off or restart the system since otherwise this can lead to damage to the system Special Laser Safety Equipment Laser Protection Tube and Laser Pr
67. itch the power off on the compact supply unit the power plug must be disconnected Flexible Supply Unit The main circuit breaker is located on the right rear side of the flexible supply unit It is used to de energize the complete system using a single switch see Figure 25 The main circuit breaker functions as a switch and as an overcurrent fuse The main circuit breaker is not to be used as the regular on off switch for the system The supply unit must be set up so that the main circuit breaker is freely accessible at all times 000000808900 000000000000000000 0 8090900888888800000 8888888888888888 J99000000000000000 008000008800 000000808900 080000000898000880 6 000000810098889 900 PS BB SSS JE 00009000000000 2098900000850 of Figure 25 Rear side of the flexible supply unit with the main circuit breaker 55 56 10 2 10 2 1 10 2 2 Key Switch Master Key Switch on the Compact Supply Unit There is a master key switch on the compact supply unit for protection against unauthorized use of the laser products This master key switch is integrated in the interlock circuit If the master key switch is in the off position all laser beam paths are interrupted Then no radiation from lasers integrated in the supply unit or the external lasers can reach the specimen area even if the key switches of the external
68. itional note This note serves to emphasize important instructions for handling the product or contains special instructions about a certain topic General Safety Notes You have to follow the instructions listed below to work with the instrument safely and without disturbance If you do not follow these or other instructions in this User Manual or the included manual Leica Microsystems CMS GmbH shall not be liable for any resulting injury or property damage As itis impossible to anticipate every potential hazard please be careful and apply common sense when using the system Commissioning and Use e he system components have been packaged securely for transport in multiple crates Do not open these crates The crates may be opened and unpacked by Leica service technicians or by people who are authorized by Leica Microsystems CMS GmbH only e he system may only be set up by Leica service technicians or by people who are authorized by Leica Microsystems CMS GmbH e This laser equipment may be operated only by persons who have been trained in the use of the system and about the potential hazards of laser radiation e Have your laser safety officer instruct you about the dangers of laser radiation and about suitable laser safety precautions such as wearing suitable laser safety glasses This applies to all persons present in the room where the system is set up and operated e Each user must have read the instructions included and follo
69. l beep warns the user if the detector is being operated near the maximum permitted signal level If the maximum permitted signal level is exceeded the detector automatically switches off and the red status LED on the detector module see Chapter 12 3 Figure 79 item 2 lights up If you have any further questions please directly contact the Leica branch office in your country see Chapter Contact 6 3 1 Objectives 24 Only use immersion fluids that are intended for the objective Unsuitable immersion fluid can contaminate or destroy the objective When changing over from an oil or water objective to a dry objective you have to remove the immersion medium from the specimen slide in order notto damage the dry objective Never open the objectives for cleaning If there is a piezo focus installed on your system be absolutely certain to observe the corresponding notes in Chapter Using a Piezo Focus 7 1 1 7 1 1 System Overview and Properties TCS SP8 System Variants TCS SP8 with Upright Microscope and Compact Supply Unit Figure 2 TCS SP8 with Upright Microscope and Compact Supply Unit 1 Upright microscope 5 Compact supply unit 2 Scan head 6 Control panel 3 SmartMove 7 Fluorescence lamp EL6000 4 Workstation 8 Microscope electronics box 25 26 Figure 3 Dimensions of TCS SP8 with upright microscope and compact supply unit 1 1 2 TCS SP8 with Upright Microscope and Flexible Supply Unit
70. le if you select an experiment by right clicking it Experiments Acquisition Experiment 5 Experiment Series002 Close Experiment Save Experiment Save All Save Experiment as Create Collection Delete Rename Cut Copy Export As Tiff Properties As JPEG Open in new viewer As QuickTime Close All As As MPEG 4 As WMV As DICOM As ASCIL Figure 83 Possible export formats 13 3 13 3 1 13 32 LAS AF Online Help Structure of Online Help Online Help is divided into seven main chapters General Information Contains legal notices and general information on LAS AF About LAS AF Online Help Contains general information for the LAS AF Online Help LAS AF Steps Contains detailed dialog descriptions for the user interface in LAS AF steps LAS AF Application Wizards Contains detailed dialog descriptions for the user interface in LAS AF application wizards and extensive documentation for the MatrixScreener wizard LAS AF Advanced Time Lapse Modes Contains detailed dialog descriptions for the user interface in live data mode and in ElectroPhysiology operating mode LAS AF Express View Contains a detailed description of the LAS AF express view file browser Additional Information Contains background information on topics related to LAS AF applications and systems such as digital image processing dye separation and objective accessories Accessing Online Help
71. m 13 1 provided by default Diameter of adapter piece 15 cm 6 Air flow rate 400 m3 h 14 126 ft3 h The remaining waste heat 4 6 kW 4 7 kW must be extracted otherwise the room temperature will rise In order to maintain a constant room temperature a high performance air conditioning system should be used TCS SP8 MP with compact supply unit Waste heat of the complete 4 7 kW system HyD RLD 100 W 49 50 9 6 5 8 6 6 TCS SP8 X Waste heat of the complete 3 6 kW system Including the argon laser and white light laser Waste heat of the argon laser 1 6 kW For cooling the argon laser must be connected to the provided argon laser fan The following space Is required Standard fan about 30 x 30 cm 1 x 1 Noise reduced laser fan 50 x 60 cm 1 8 x 2 In addition itis possible to connect the argon laser exhaust to an in house exhaust system Hose length 400 cm 13 1 provided by default Diameter of adapter piece 15 cm 6 Air flow rate 400 m3 h 14 126 ft3 h The remaining waste heat 2 kW must be extracted otherwise the room temperature will rise External Lasers Observe the user manuals for external lasers BA Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 9 9 1 9 2 9 21 Laser It is mandatory to observe the laser safety measures for las
72. m space requirements length x depth TCS SP8 with upright 350 x 280 cm 11 6 x 9 2 microscope TCS SP8 with inverted 373 x 280 cm 12 3 x 9 2 microscope TCS SP8 MP with DM6000 and 440 x 280 cm 14 5 x 9 2 IR laser without precompensation TCS SP8 MP with DM6000 and 500 x 280 cm 16 5 x 9 2 IR laser with precompensation TCS SP8 MP with DMI6000 and 370 x 360 cm 12 2 x 1110 IR laser without precompensation TCS SP8 MP with DMI6000 and 450 x 290 cm 14 9 x 9 6 InSight DeepSee laser single TCS SP8 MP with DMI6000 and 420 x 350 13 9 x 11 6 InSight DeepSee laser dual TCS SP8 MP with DMI6000 and 500 x 280 cm 16 5 x 9 2 IR laser with precompensation TCS SP8 X with upright 400 x 280 cm 13 1 x 9 2 microscope TCS SP8 X with inverted 423 x 280 cm 13 10 x 9 2 microscope TCS SP8 with external UV laser 482x280 cm 15 10 x 9 2 and upright microscope TCS SP8 with external UV laser 505 x 280 cm 16 7 x 9 2 and inverted microscope 8 4 Electrical Connection Requirements WARNING _ Electric shock is possible when using ungrounded sockets This system is designed for connection to grounded earthed outlets The grounding plug performs an important safety function To avoid the risk of electrical shock or damage to the instrument do not disable this feature Operation without grounded sockets is not permitted 8 4 1 TCS SP8 with flexible supply unit Supply voltage 100 V to 240 V
73. mpact or flexible 4 Beam coupling unit 5 IR laser with dispersion compensation 2 Optical table 180 x 120 cm 5 11 x 3 11 6 Power supply and cooling of the IR laser 3 Microscope DMI6000 33 34 7 2 5 TCS SP8 MP with DMI6000 and InSight DeepSee laser 1 5 m 4 11 Figure 14 vSystem overview of the TCS SP8 MP with DMI6000 and InSight DeepSee laser dual 1 Stage with supply unit compact or flexible 2 Power supply and cooling of the IR laser 3 4 5 6 Microscope DMI6000 Beam coupling unit InSight DeepSee laser dual Optical table 1 5m x 1 8m 4 11 x 5 11 1t 8m 5 11 Figure 15 System overview of the TCS SP8 MP with DMI6000 and InSight DeepSee laser single 1 Stage with supply unit compact or flexible 2 Power supply and cooling of the IR laser 3 4 5 6 Microscope DMI6000 Beam coupling unit InSight DeepSee laser single Optical table 1 8m x 1 2m 5 11 x 3 11 35 36 1 3 7 3 1 TCS SP8 X System Variants TCS SP8 X with Upright Microscope Figure 16 TCS SP8 X with upright microscope 1 Upright microscope 6 Flexible supply unit 2 Scan head 7 Control panel 3 SmartMove 8 Fluorescence lamp EL6000 4 Workstation 9 Microscope electronics box 5 1 Main switch board 0 White light laser Figure 17 Dimensions of TCS SP8 X with upright microscope 1 3 2 TCS SP8 X with Inverted Microscope Figure 18 TCS S
74. nant Copyright 1997 2012 Leica Microsystems CMS GmbH Cancel Figure 80 Selection of resonant or conventional mode LAS AF saves the settings for Configuration Microscope and Resonant as they were set the last time the software was started 3 Now start LAS AF by clicking the OK button You now in the main LAS AF view 93 94 132 Structure of the Graphical User Interface Quantity Autofocus Uve Figure 81 Structure of the Graphical User Interface E 1 Scaling range 7 Image display 2 Operating mode selection 7a Image display settings 3 Steps 7b Image processing and image analysis options 4 Menu 7c Channel display 5 Tab area 8 Image acquisition buttons 6 Working area Scaling range Using the slider you can modify the size display of the user interface On the right next to the slider there is a reset button which you can use to reset the scaling to the default setting Operating mode selection Here you can set the operating mode you would like to use for image acquisition and analysis independently of your system configuration Steps Various steps are available depending on the selected operating mode The TCS SP8 base module has the following four steps Configuration Acquire Process Quantify Menu The File and Help dropdown menus are located here Tab area The displayed tabs depend on the selected step In the TCS SP8 s basic operating mode the following tabs are assigned to
75. ne diol Corrosion inhibitors Content w w gt 25 5 EC No 203 473 3 INDEX No 603 027 00 1 CAS No 107 21 1 Hazard symbol Xn R phrases 22 CAS No 19766 89 3 Hazard symbol Xn R phrases 63 Content w w 0 5 0 75 EC No 243 283 8 The wording of the hazard symbols and R phrases is specified in Chapter 16 if dangerous ingredients are mentioned 3 Hazard identification Special risks for people and environment Damages caused to someone s health by swallowing 4 First aid measures General advice If inhaled On skin contact On contact with eyes On ingestion Note to physician 5 Fire fighting measures Suitable extinguishing media Special protective equipment Further information Figure 116 Remove contaminated clothing If difficulties occur after vapour aerosol has been inhaled fresh air summon physician Wash thoroughly with soap and water Wash affected eyes for at least 15 minutes under running water with eyelids held open Rinse mouth immediately and then drink plenty of water seek medical attention Symptomatic treatment decontamination vital functions Administer 50 ml of pure ethanol in a drinkable concentration Water spray alcohol resistant foam dry extinguishers carbon dioxide CO2 In case of fire wear a self contained breathing apparatus The degree of risk is governed by the burning substance and the fire conditions Contaminated extinguishing w
76. nsmitted light arm back Replace the specimen Insert the specimen correctly into the specimen holder tr Tilt the transmitted light arm back into the working position 101 102 15 Changing the Objective WARNING _ Risk of permanent eye and skin damage from laser radiation A radiation can escape uncontrolled from the specimen area Never change objectives during the scanning operation because laser To change objectives proceed as follows 1 2 3 WARNING _ Risk of permanent eye and skin damage from laser radiation Finish the scanning operation Switch off the internal lasers using the detachable key switch If any external lasers are present switch them off with their detachable key switch or as described in the laser manufacturer s user manual Rotate the objective nosepiece so that the objective to be changed is swiveled out of the beam path and points outward Exchange the objective Close all unoccupied positions in the objective nosepiece using the supplied caps System operation with unlocked positions in the objective nosepiece is not allowed closed with the caps provided in order to prevent the uncontrolled All non occupied positions in the objective nosepiece must be escape of laser radiation in the specimen area 103 Changing the Objective 104 16 Piezo Focus on Upright Microscope If there is a piezo focus installed on your system see Figure 86 pay careful atte
77. ntion to the following notes Figure 86 Piezo focus on objective nosepiece Make sure that the specimen carrier is not againstthe objective and cannot be damaged by it or cause broken glass The objective could likewise be damaged Before switching the system on or launching the LAS AF software ensure that there is no slide or specimen on the stage and that the specimen stage Is in its lowest possible position If this is not observed specimens and objectives can be damaged or destroyed upon system software startup by the initialization of the piezo focus Do not carry out the objective change automatically The automatic motion may damage the cable of the piezo focus Do not make any adjustments to the piezo focus controller see Figure 87 as it has already been optimally set up by Leica Service When replacing the objective on the piezo focus you must perform a teach in for the new objective in LAS Please see the instructions on this topic in the microscope operating manual Please note that the focus position of an objective with piezo focus is 13 mm lower than those without piezo focus In order to guarantee the same focal plane a spacer Is installed on all other objectives see Figure 88 The objective can be moved by 150 um in either direction The total travel is 300 um Piezo focus controller display Highest position 350 um Middle position 200 um Lowest position 50 um xz scan range 250 um 106 Figure 8
78. nts IEC EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements class A This is a Class A instrument for use in buildings that do not include domestic premises and buildings not directly connected to a low voltage power supply network that supplies buildings used for domestic purposes For use in the USA CDRH 21 CFR 1040 10 Laser Products U S Food and Drug Administration FDA Complies with FDA performance standards for laser products except for deviations pursuant to laser notice No 50 dated June 24 2007 For the USA area of validity of the CDRH FDA the designations of the laser class are to be changed in the text from to 1116 and Class 4 to IV 137 EC Declaration of Conformity Seica No CE 137 01 MICROSYSTEMS Manufacturer Leica Microsystems CMS GmbH Address Am Friedensplatz 3 68165 Mannheim Germany Product Confocal Laser Scanning Microscope TCS SP8 product family and accessories TCS SP8 X product family and accessories We declare that the product described herein complies with the following European Directives 2004 108 EC Directive on Electromagnetic compatibility 2006 95 EC Directive on Low voltage equipment The product conforms to the standards EN 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements Emission Class A EN 610
79. oling requires approx 4 hours to regulate the temperature of the infrared laser 06 2 After 4 hours switch on the supply unit for the infrared laser see Figure 67 item 2 t Figure 67 Cooling and supply unit for the InSight DeepSee infrared laser 3 Ilf you are using a fluorescence lamp EL6000 switch it on first Figure 68 EL6000 4 f you are using a RLD switch it on at its supply unit You can find additional information on the HyD RLD in Chapter 12 3 Figure 69 HyD RLD supply unit 07 5 7 8 88 Switch on the workstation The operating system starts automatically Wait until the startup process has concluded Turn on the microscope by actuating the electronics box toggle switch Figure 70 item 2 The microscope is operating once the readiness indicator Figure 70 item 1 on the toggle switch is lit up Figure 70 Microscope electronics box Switch on the microscope s control panel Figure 71 Control panel for the microscope If your system has an infrared laser MP or an optical parametric oscillator OPO switch on the NDD detection unit Figure 72 Switch on the NDD detection unit 9 Switch on the scan head on the front side of the compact supply unit see Figure 73 item 1 Scanner Laser Laser Power Power Emission s 1 3 Figure 73 Overview of
80. om must be free of dust oil and chemical vapors After installing the system you may carry out interior finish work on the room only if the system is stored in a dust free location while this work is underway Avoid direct sunlight and vibrations since these can distort measurements and micrographic scans We recommend using a room that can be completely darkened Do not expose the system to drafts If the system has to be moved to a new location for any reason contact the Leica branch office in your country Using the Software Before carrying out operating steps with the system first read the corresponding description of the function in LAS AF Online Help For an overview of the individual functions refer to the table of contents of the online help Back up your data regularly to a suitable data carrier Do not install any hardware or software on the workstation as otherwise serious damage to the system or loss of data can result Do not switch the workstation off after a software crash but restart the LAS AF software after 15 seconds No image data are lost in case of a software crash If the LAS AF software is restarted without restarting the workstation the data are automatically restored If the software crash is caused by a crash of the workstation the image data Will be lost 23 6 3 Protecting the System Observe the maintenance instructions and intervals prescribed in the Chapter Maintenance During the upd
81. or the compact supply unit before the shut off argon laser has cooled down over the ventilation since it can incur damage otherwise Close the LAS AF software On the menu bar select File Exit File Help New Experiment Open Expenment Close Experiment Print Print Setup Print Preview Figure 92 Shutting down LAS AF Turn off the lasers in the supply unit with the key switch on the main switch board of the flexible supply unit see Figure 93 item 4 Scanner Laser Microscope Power Power Emission Figure 93 Overview of the main switch board on the flexible supply unit The emission warning indicator goes out 6 If your system has a white light laser check whether the emission warning indicator is lit If the emission warning indicator on the front side of the white light laser is lit press the key below it This causes all internal white light laser components to shut off and the emission warning indicator to go out 109 Figure 94 Emission warning indicator on the white light laser 7 Switch off the white light laser with the key switch on the front of the white light laser Figure 95 Key switch for the white light laser 8 If you are using external 355 nm UV laser including 405 nm wavelength activate the following key switch to turn it off The emission warning indicators go out Figure 96 Key switch on the power supply of external 355 nm UV laser 110
82. ordance with the essential require ments of safety precautions and physical toxicological and ecological data 2 how to handle store use and transport them safely No liability for damage occured in connection with the use of this information or with the use application adaption or processing of the products here described will be accepted An ex ception will be made in the case that our legal representatives should come to be held re Figure 119 innovatek Protect application mixture safety data sheet page 4 135 136 SAFETY DATA SHEET Gem 91 155 EG 2001 58 EG n ovatek TE gt innovatek OS GmbH Dated www innovatek de February 28st 2011 innovatek Protect application mixture info innovatek de sponsible and liable by reason of intent or gross negligence No liability will be accepted for damage indirectly incured We provide this information and data according to our present level of knowledge and expe rience No assurances concerning the characteristics of our product are hereby furnished Figure 120 innovatek Protect application mixture safety data sheet page 5 24 3 Compliance This system has been tested and meets the requirements of the following standards IEC EN 61010 1 2011 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements IEC EN 60825 1 2007 Safety of laser products Part 1 Equipment classifications and requireme
83. otection Shield The laser protection tube and the laser protection shield are used in inverted microscopes for protection from laser radiation see Figure 41 Figure 41 Inverted microscope 1 Laser Protection Tube 2 Condenser Base 3 Laser Protection Shield 65 66 10 7 2 Reordering a Condenser Base When reordering a condenser base see Figure 41 item 2 be sure to note that the condenser base is supplied without a laser protection shield see Figure 41 item 3 The existing laser protection shield see Figure 41 item 3 must always be reinstalled Please consult the microscope s user manual provided Condenser Base with Filter Holder When using a condenser base with filter holder always make sure that unused filter holders are swung out of the beam path and that the laser protection tube covers the beam path When equipping multiple filter holders with filters do so from bottom to top so that the laser protection tube can cover the beam path to the greatest possible extent Do not swing in the filters during the scanning operation Safety Beam Guide on the TCS SP8 MP The light of all employed VIS lasers wavelength range 400 700 nm visible spectrum and UV lasers wavelength range 400 nm invisible is fed through a fiber optic cable and therefore completely shielded until it leaves the microscope objective and reaches the specimen For systems with infrared laser wavelength range 700 nm t
84. personalized user ID 9 Use your personal user ID if one has been set up This ensures that user 1 specific settings saved and maintained for this user only the system administrator has not yet assigned a personal user ID log on as CS User The default password is True Confocalb5 90 12 3 Change password After logging on with your own user ID you may change your password 1 To do so press the Ctrl Alt and Del keys at the same time Then click Change password The Change password dialog box opens Now you can start the LAS AF software HyD Reflected Light Detectors HyD RLDs If you are using HyD RLDs you have to switch them on before LAS AF starts Otherwise the LAS AF software cannot initialize the detectors The HyD RLDs consist of a supply unit see Figure 78 and a detector module see Figure 79 e he power switch for switching on and off the power supply and the cooling for the detector module is located on the front side of the supply unit see Figure 78 e For image acquisition activate the detectors in LAS AF The yellow status LED see Figure 79 item 2 flashes if photons are being detected Figure 78 HyD RLD supply unit 91 92 Figure 79 HyD reflected light detectors HyD RLDs Status LED color see Figure 79 item 2 Gren Operational readiness readiness The maximum CI a signal level was exceeded The
85. r UV Lasers 0 0c ccc cece IRI 57 10 25 Key Switches for Other External 50 10 3 Emission Warning 58 10 3 1 Emission Warning Indicator on the Supply 58 10 3 2 Emission Warning Indicator at the White Light Laser 59 10 3 3 Emission Warning Indicator on UV Lasers 59 10 3 4 Emission Warning Indicator on Other External Lasers 60 10 3 5 Malfunction of Emission Warning 60 10 4 Interlock Connector S ou pseudo Uter wi A aah DIRE DERE eb rnb mets 60 10 4 1 Interlock Connector on the Supply 60 10 4 2 Interlock Connector on the White Light 62 10 4 3 Interlock Connector on Other External 63 10 4 4 Interlock Connector on the Scan 63 10 5 Safety Switches on the Microscope 64 06 Waring MessdgBsus exces vir d Ew Gan EX EESLRLLIqESENG S IE OU bets 64 10 7 Special Laser Safety 65 10 7 1 Laser Protection Tube and Laser Protection Shield 65 10 7 2 Safety Beam Guide on the TCS SP8 66 11 Safet
86. re Operation without grounded sockets Is not permitted 21 22 5 6 9 7 Make sure that the supply voltage at the system remains in an approved tolerance range 100 V 240 V 10 The system may be connected to a power supply with ground protection conductor only Do not interfere with the grounding function by using an extension cord without a ground wire Any interruption of the ground wire inside or outside of the system or release of the ground wire connection can cause the system to become hazardous Intentionally disconnecting the ground protection conductor is not permitted Before any cleaning or servicing de energize the entire system To do so use the power switches of all components and disconnect all power cables from the power supply Only use the power cable included or provided by your local Leica service technicians for connecting individual peripheral devices to the power supply Fuses inside the system may be replaced only by authorized Leica service employees If you have any further questions please directly contact the Leica branch office in your country Contact with Liquids To avoid the risk of electrical shock and fire hazards never expose the system to rain or moisture Do not allow any liquid to enter the system housing or come into contact with any electrical components Avoid condensation The system must be completely dry before connecting it to the power supply or turning It on Do
87. right Contents e pcc 3 Contents 6 5 cen cee aie zoe 5 1 About this User Manual eee eee eee eens 11 2 ONGC W USC wees nd eden Ga ee ad ene 13 3 Liability and Warranty 0 ccc ccc cece ee eee I IK Ihn 15 3 1 Important Information for Operators and 15 4 Meaning of the warning messages in the 17 5 General Safety 19 5 1 19 5 2 Modifications to the System 19 53 Safety Devices and Safety wii ect RR Ee er veces vc os 20 BA Laser Sale diede eA 20 D gt AEIBCEHIOIUS dEB DV euius e inta cm a idi ie ie cR a aet p EUR b eh eR SA 21 56 Contact with Eigulds ss x e E br EE wince a OO 22 5 7 Malfunction ofthe Systemi iretsi icenen IRI In 22 6 Additional Notes on Handling the 23 En a ee eC SR ee eee eee 23 62 USING tie oOI We on iv saccades chad dca is PAM Ont oatre 23 03 Protecung NE SVSEBITES uocari adire De p EORR D essen vere ge e ban err ei 24 6 3 1 TN CCT Sia erste obama Eo 24 7 System Overview and
88. rly laser radiation in the specimen area If any of these occur immediately notify the Leica branch office in your country or your local contact person 10 4 Interlock Connectors 10 4 1 Interlock Connector on the Supply Unit The interlock connector plug connection for the remote controlled safety interlock is on the rear side of the supply unit Operating voltage is 12 V DC for the flexible supply unit see Figure 37 for the compact supply unit see Figure 36 The interlock connector on the supply unit has a shorting plug in its factory condition The shorting plug is removable in order to connect a remote controlled safety interlock cable and safety switch The laser beam path is interrupted if the contact is open The safety interlock can for example be connected to a door contact When the door is opened the laser beam is then interrupted automatically The total length of the cable must not exceed 10 m If a large amount of electromagnetic interference EMC is expected in the environment use a shielded cable with a shielded plug Figure 36 Interlock connector on the compact supply unit 9 19402080800 9800000 0080000600 Figure 37 Interlock connector on the rear side of the flexible supply unit 61 10 4 2 Interlock Connector on the White Light Laser The interlock connector is located on the rear side of the white light laser 12 V DC operating voltage see
89. s Figure 29 Key switch on the power supply of external UV laser 355 Figure 30 Key switch on external UV laser 405 5 58 10 2 5 10 3 10 3 1 Key Switches for Other External Lasers Observe the user manuals for external lasers BA Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes Emission Warning Indicators The operational readiness of lasers is signaled by an emission warning indicator WARNING Possible risk of permanent eye and skin damage from laser radiation in the specimen plane As soon as the emission warning indicator of the lasers is lit it is possible A from a functional standpoint that laser radiation is present in the specimen area Emission Warning Indicator on the Supply Unit The emission warning Indicator on the supply unit is connected to the master key switch If this is illuminated there is the possibility of laser radiation in the specimen plane If the master key switch is in the off position the emission warning indicator the supply unit goes out The emission warning indicator is located on the supply unit above the key switch and it lights up in yellow for compact supply units see Figure 31 for flexible supply units see Figure 32 Scanner Laser Power Power J 6 Figure 31 Emission warning indicator on the compac
90. s such as color Image processing and image analysis options This area contains buttons that can be used to add information to the image such as text arrows or dimensions In addition there is a button that enables regions of interest ROI to be plotted in the image display Channel display Here you can select how the acquired image is to be displayed If you have excited your specimen with multiple laser lines you can use the buttons to select whether the different channels are displayed individually next to each other or in one image 95 96 Image acquisition buttons These buttons are available to you during the Acquire and Quantify steps Here you can select how the acquisition is to be started e Autofocus Focusing using the selected settings Live Acquisition of a live image Capture Image Acquisition of an individual image e Start Series of acquisitions using the selected settings The software s experiment concept allows logically interconnected data to be managed together The Experiments tab is displayed in a tree structure in different steps Experiments are managed there Experiments Acquisition Experiment gt New Open SaveAll Apply Browse Use Default Image Base Name Figure 82 View of the Experiments tab Experiments have an export function for opening images and animations in an external application The following selection of export formats is availab
91. t supply unit ggg Scanner Laser Laser Microscope Power Power Emission Figure 32 Emission warning indicator on the main switch board of the flexible supply unit 10 3 2 Emission Warning Indicator at the White Light Laser The emission warning indicator of the achromatic light laser is located on the front of the achromatic light laser see Figure 33 and is red when lit Figure 33 Emission warning indicator at the white light laser 10 3 3 Emission Warning Indicator on UV Lasers Figure 34 Emission Warning Indicator on Power Supply of External UV Laser 355 59 Figure 35 Emission Warning Indicator on External UV Laser 405 10 3 4 Emission Warning Indicator on Other External Lasers Observe the user manuals for external lasers BA Please refer to the information from the documents provided by the laser manufacturer for the external lasers Pay particular attention to the laser manufacturer s notes 10 3 5 Malfunction of Emission Warning Indicator WARNING Malfunction of emission warning indicator You must immediately disconnect the system from the power supply if any of the following occur e The emission warning indicator is not lit after being switched on using the detachable key switch e he indicator continues to be lit after being switched off using the keyswitch e Scanning of the specimen is not activated after being switched on prope
92. thout Dispersion Compensation TCS SP8 MP with DM6000 and IR Laser See Figure 11 with Dispersion Compensation TCS SP8 MP with DMI6000 and IR Laser See Figure 12 without Dispersion Compensation TCS SP8 MP with DMI6000 and IR Laser See Figure 13 with Dispersion Compensation TCS SP8 MP with DMI6000 and InSight See Figure 14 DeepSee laser dual TCS SP8 MP with DMI6000 and InSight See Figure 15 DeepSee laser single TCS SP8 X with upright microscope 280 x 110 x 175 cm 9 2 x 3 7 x 5 9 TCS SP8 X with inverted microscope 303 x 110 x 140 cm 9 11 x 37 x 4 7 TCS SP8 with external UV laser and 362 x 110 x 175 cm 11 11 x 37 x 5 9 upright microscope TCS SP8 with external UV laser and 385 x 110 x 140 cm 12 6 x 37 x 4 7 inverted microscope Dimensions of the supply unit length x depth x height Compact Supply Unit 38 x 54 x 50 cm 1 3 x 1 9 x 1 8 Flexible Supply Unit 110 x 70x 90 cm 37 x 2 4 x 2 11 Weight of the Supply Unit Compact Supply Unit 40 kg 88 Ibs Flexible Supply Unit 90 kg 198 Ibs 7 6 3 1 1 Electrical Specifications EE Flexible Supply Unit Compact Supply White light laser Unit Supply voltage 100 V to 240 V 10 grounded Power consumption 2x 1600 VA 700 VA 400 VA Including peripheral devices connected to the flexible supply unit s multiple socket outlet see Chapter 8 5 Fuse LS automated process 2x T8AH 250 V AC LS automated process for TCS SP8 2x TAAH 250 V AC
93. vice 121 20 Maintenallbe rie aicut ead ed acm we e aa 123 20 1 Having Coolant 123 21 Disassembly and lt 125 22 Disposal 23 Contact 24 Appendix 24 1 24 2 24 3 24 4 Patents Safety Data Sheets from Third Party Manufacturers Compliance People s Republic of China About this User Manual Prior to commissioning the system carefully read through this User Manual and be absolutely certain to follow the safety notes contained in it So that you can operate the system safely and react quickly and correctly in the event of an emergency you must familiarize yourself with the safety devices before using itfor the first time In this case read Chapter Safety Features in this manual Keep this User Manual and the included manuals for the microscope and other components in a safe place easily accessible for all users This Manual gives you important information about safe handling of the system All information is intended for the safety of users and trouble free operation of the system Unless the information pertains specifically to certain system variants the instructions always apply to the basic system described here This User Manual provides you with important
94. w the instructions it contains Do not introduce any flammable objects such as paper into the specimen area when you are working with a laser Donot place any flammable or combustible objects on or near the system and do not put it near hot surfaces e During start up and during operation you have to keep your hands and fingers away from the specimen area as otherwise there is a risk of crushing hazards or injury from rotating objectives and the motorized specimen stage e Set up the workplace for example chair and monitor on the system so that it corresponds to your requirements Observe the national regulations for occupational safety e Before each service call by a service technician or whenever you relocate the instrument you have to clean it thoroughly This is necessary to remove any possible contamination thereby preventing the transfer of dangerous substances and pathogens and avoiding hazards and dangers The same also applies to the removal of components This applies in particular to systems that are located in biomedical research labs e You must not deviate from the operating and maintenance instructions provided herein Modifications to the System e The system is installed by service technicians from Leica Microsystems CMS GmbH You must not change the position of the system components 20 5 3 5 4 The supply unit must always be set up and operated in an upright position Under no circumstances may
95. y Labels the System 0c ccc eee eee eee eee 0 00 67 ILI dnverted microscopi 2 4 9 ak So Le p Senne niet eases eee RAE S 68 TIE Z Jes i vx ear a eerie ac n ee cec EO RU Re aite ont es 10 OC MMC auton dace Jang ee dda asec a easton ae Se 72 Compact erie eer beeen dE a 74 11 5 Flexible Supply UMN Geet aise C i eod iem aora 74 11 6 MP Beam Coupling 15 11 7 Cover for replacement flange 16 118 MUFEOE THOUSITI sa o rus oC ede RP els doin E Dad e REPE Erde ILES White Digit lasers Ed ote ci dead 18 EXC alu noted oo aO E RUPEE ER PEORES SUR EPOR ORE I NR 18 ETT ther External su ioa react d deep e PU o e ROS 19 12 Switching the System LLLsuuueeeeee eee nn nnn 81 12 1 TCS SP8 SP8 X with Flexible Supply 81 122 TCS SP8 with Compact Supply 86 123 Reflected Light Detectors 05 91 NS AF ree ta ee eee een eee ub M MS SEE 93 Tou LAS Ar oo Coa eire Bes r eR IH Pe Padi d Ve AR 93 13 2 Structure of the 94 13 3
96. you open housing parts Never disconnect a fiber optic cable The cable and fiber optic cable may not be folded stretched pinched or rolled up tightly or damaged in any other way The product has a closed liquid coolant circuit The cooling liquid hoses may not be folded stretched pinched or rolled up tightly or damaged in any other way Do not connect any external equipment or other components If you have questions please directly contact the Leica branch office in your country Safety Devices and Safety Labels So that you can operate the system safely and react quickly and correctly in the event of an emergency you must familiarize yourself with the safety devices before using it for the first time Read Chapter Safety Features of this User Manual carefully Never remove the safety devices on the system Never deactivate the laser protection devices All safety devices must be ready to operate Do not carry out any procedures that modify disable or damage the functionality of safety features Unauthorized procedures could result in serious injuries or property damage Safety labels on the system may not be removed Missing or damaged safety labels must be attached immediately and at the described location Observe Chapter Safety Labels on the System Laser Safety The instrument is a Class 3B IIlb VIS and UV lasers or a Class 4 IV IR lasers laser product You must observe all suitable safety measures applicable for

Copyright

Contents

Download Pdf Manuals

Related Search

Related Contents