Electrophoresis Power Supply EPS 3501 XL
Contents
1. Phase number Voltage V Current mA X Power W Volthours Vh 1 500 1 5 001 1 2 500 1 5 5 2500 3 3500 1 5 5 10000 4 3500 1 5 9 5 33250 The ramping from 0 to 500 V should be done as quickly as possible The smallest possible time that can be set is 1 minute and the smallest possible Vh that can be set is 1 Vh Vh was chosen for this program as breakpoint unit Choose phase number Choose phase number with w A and confirm with set enter For a new program the default phase number is 1 If the first phase has been programmed and the question Last phase is answered by No N the default number is 2 and so on Setting voltage current and power The display will now flash for the set voltage limit STEP mode or voltage endpoint GRADIENT mode Using the v keys select the voltage limit or voltage endpoint desired for the run Confirm with set enter Repeat the same procedure for limiting current and limiting power Programmable values for voltage are 35 3500 V current 1 400 mA power 1 200 W Setting breakpoint Choose between automatic or manual break For automatic break choose breakpoint unit in either hours h volthours Vh or milliamperehours mAh Select the correct unit or for manual break choose OFF with v Confirm with set enter Note that the breakpoint unit is valid for all phases within the program and you will only get this question when programming the first phase see Other phases bypas2. 2009 General Electric Company All rights reserved 751 84 Uppsala First published Oct 2009 Swede n All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information www gelifesciences com GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan imagination at work 28 9639 69 AA 10 2009
3. 9 SET 2n 1000 Vh Last phase D Y Yes No 10 Alarm Phase 1 N Phase Select by Confirm by y ALV vs 11 SET yas 1000 Vh SET aya 1000 Vh 1000 V 10mA 10W 1000 V 10mA 10W 28 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Operation 4 Setting uparun 4 3 Step Action SEIBCIIOTIS 1 Choose Program number 2 2 Choose STEP or GRADIENT mode STEP 3 Confirm Phase number 1 4 Set Voltage limit in Step mode or Voltage endpoint 1000V in Gradient mode 5 Set Current limit 10 mA 6 Set Power limit 10W 7 Choose Breakpoint unit h Vh or mAh or no breakpoint Vh OFF OFF 8 Set Breakpoint 1000 Vh 9 Is this the last phase Last Phase Choose Yes Y or No N 10 Choose Alarm Off N or On Y Y 11 The program is ready Selection made in this example Figure 4 1 Step by step summary of programming Choosing step or gradient programming Press set enter to confirm STEP or use vA keys to choose GRADIENT Confirm with set enter Note that your choice of gradient or step programming applies for all phases within the program and you will only be asked to choose one of them when programming the first phase Choosing step means that voltage current and power limiting values are programmed The electrophoresis will be controlled by one of these limiting values which means that itis
4. an electrophoresis unit incorrectly connected use of buffer with exremely low conductivity This function can be disabled to perform certain applications see Electrophoresis Power Supply EPS 3501 XL User Manual 20 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Safety instructions 2 Recycling information 2 5 Function Description Sudden load This function prevents accidents under running conditions change detection due to a break in the electrical circuit such as a bad connection to the electrophoresis unit The high voltage is turned off in such an event An error messages is shown on the display in the event of a sudden load change 2 5 Recycling information The equipment shall be decontaminated before decommissioning and all local regulations shall be followed with regard to scrapping of the equipment Disposal general instructions When taking EPS 3501 XL out of service the different materials must be separated and recycled according to national and local environmental regulations Recycling of hazardous substances EPS 3501 XL contains hazardous substances Detailed information is available from your GE Healthcare representative Disposal of electrical components Waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for inf
5. 69 AA Operation 4 Setting uparun 4 3 endpoint The electrophoresis will be controlled by this voltage gradient provided the limiting current or power is not attained The EPS 3501 XL thus automatically switches over the controlling parameter according to the programmed limits and conductivity variations in the system 3500 4 D 33250 Vh 10000 Vh 500 2500 Vh 5 10 19 5 F No Description No Description A Phase 1 D Phase 4 B Phase 2 E Voltage V C Phase 3 F Time h Figure 4 3 Programming the voltage limiting profile in GRADIENT mode The parameters shown are the same as those listed in Table 4 2 To illustrate voltage gradient programming the programming for the IEF part of a 2 D electrophoresis with Immobiline DryStrip Gels is shown in Table 4 2 and Figure 4 3 Note that the first phase is a very steep gradient to reach the 500 V start level 0 500 V within 1 Vh The next phase is actually a step since the endpoint for phase 2 is 500 V which is the same as the endpoint for phase 1 Phase 3 isthe real gradient the voltage is changed from 500 to 3500 V in 5 hours The last phase is a step again the endpoint voltage is the same as for the phase before and the voltage will remain on 3500 V for 9 5 hours Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 31 4 Operation 4 5 Setting up a run 32 Table 4 2 The parameters of a gradient program
6. EPS 3501 XL is primarily designed for techniques using programming in several phases and or voltage gradients e 2 D Two dimensional electrophoresis using Multiphor Il Electrophoresis System e EF Isoelectric focusing using Immobiline or carrier ampholytes such as CleanGel and ExcelGel e Flatbed SDS PAGE Polyacrylamide Gel Electrophoresis or flatbed native PAGE EPS 3501 XL is also suitable for e DNA sequencing e Agarose electrophoresis e Electroblotting e DNA pulsed field electrophoresis Nine programs each with up to nine phases can be saved Limiting values for voltage current and power as well as voltage gradients can be programmed for precise control of the electrophoresis The EPS 3501 XL automatically switches over the controlling parameter according to programmed limits and gradients and conductivity variations in the system Two electrophoresis units can be connected to EPS 3501 XL and run with the same programmed method at one time Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 9 1 Introduction 1 3 Instrument Instrument description Figure 1 1 The main parts of the EPS 3501 XL instrument Part Description 1 Display 2 Keyboard 3 Output sockets 10 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Indicator and switch on the instrument unit Indicator Switch HV ON indicator lamp Color Gre
7. I position When the power supply is turned on the display shows the start position in SET mode The previous program set is shown and that program number is blinking SEL 1J1 0 00h OV OmA OW If the power supply is turned on for the first time or if the program has been cleared see Optional programming on page 34 the default way of controlling is step the breakpoint parameter is time h the alarm is off and the values are all zero Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 27 4 Operation 4 3 Setting up a run 4 5 Setting up a run Programming a method Choosing a program Up to nine programs each with up to nine phases can be entered Press set enter to confirm the program shown by the number on the display or use vA keys to choose another Confirm with set enter 1 SET 1 1 1Vh 500V 1mA 5W ct by O 0 Confirm by 2 SET 2n STEP gradient lect by G1 0 Confirm by SET 271 0 00 h ov Oma OW Confirm by 4 SET 2 0 00h ov Oma OW eect IU contre 5 SET 2 0 00 h 1000 V 10mA OW Select by Ale Confirm by _ T 2 6 SET 0 00 h 1000 V Oma ow Select by 1st Phase SET 2p 0 00 h 1000 V 0 mA 10W Select by C Confirm by Vh Se o S m Confirm by _ Other phases Breakpoint OFF SET 2n 0 Vh 1000 V OmA 10W Select by ME Confirm by
8. O Positive Output socket Label Color Voltage Red O and 1750 V Negative Black or blue 0 and 1750 V Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 13 1 Introduction 1 3 Instrument 14 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Safety instructions 2 Safety precautions 2 1 2 Safety instructions This chapter describes safety compliance safety labels general safety precautions emergency procedures power failure and recycling of EPS 3501 XL 2 1 Safety precautions Introduction The EPS 3501 XL is powered by mains voltage and handles liquids that may be hazardous Before installing operating or maintaining the equipment you must be aware of the hazards described in this manual Follow the instructions provided to avoid personal injury or damage to the equipment The safety precautions in this section are grouped into the following categories General precautions Personal protection Installing and moving the instrument System operation Maintenance General precautions WARNING Do not operate the EPS 3501 XL in any other way than described in the EPS 3501 XL User Manual WARNING Operation and user maintenance of EPS 3501 XL should be performed by properly trained personnel only WARNING Do not use any accessories not supplied or recommended by GE Healthcare Electrophoresis
9. Press w a to change the parameter value or other choice in the field which is blinking Numerical values are changed in an accelerating manner when a key is held down Clicking a key changes the value in preset increments Parameters or units e g Vh and choices e g Yes No are changed with one key push The keys can also be used to switch between time and volthours in RUN PAUSE and END The values scroll i e they automatically change from maximum to minimum value or vice versa 5 e run Press run to start the run and put the program into RUN mode The current values for voltage current and power are shown on the display The elapsed time volthours or milliamperehours are also displayed Switch between these last three parameters with v pause continue pause continue Press pause continue to put the instrument in PAUSE mode and switch off the voltage The display shows the status of the run at the time the key was pressed pause continue only operates in RUN mode Time integrated voltage and integrated current are retained In the PAUSE MODE mode set enter can be used to make changes in the program Return to RUN mode by pressing pause continue or by pressing run STOP STOP Press STOP to stop the run and put the instrument in END mode The voltage is switched off and the end parameters are displayed Switch between time integrated voltage and integrated current by pressing W A A run cannot b
10. Regulatory information 1 2 Notes and tips Note A Note is used to indicate information that is important for trouble free and optimal use of the product Tip A tip contains useful information that can improve or optimize your procedures Typographical conventions Software texts and commands are identified by bold italic text A colon is used to separate menu levels e g File Open refers to the Open option in the File menu 1 2 Regulatory information This section lists the directives and standards that are fulfilled by EPS 3501 XL Manufacturing information Requirement Content Name and address of manufacturer GE Healthcare Bio Sciences AB Bj rkgatan 30 SE 751 84 Uppsala Sweden Name and ID of notified body INTERTEK SEMKO AB NB 0413 Place and date of declaration Uppsala Sweden May 2009 Identity of person authorized to sign DoC See EC Declaration of Conformity CE Conformity Directive Title 2006 42 EC Machinery Directive MD 2006 95 EC Low Voltage Directive LVD 2004 108 EC ElectroMagnetic Compatibility EMC Directive Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 7 1 Introduction 1 2 Regulatory information International standards Standard EN 61010 1 IEC 61010 1 CAN CSA C22 2 No 61010 1 Description Safety requirements for electrical equipment for measurement control and laboratory use Notes EN 61326 1 EMC emi
11. a time integrated voltage or current that is already passed Press exit or pause continue to go back from P SET to PAUSE Press run or pause continue to proceed with the electrophoresis Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 37 4 Operation 4 5 Performing a run 38 1 SET 1 1 1Vh 500V 1mA 5W Select by q U 2 SET 211 1000Vh 1000V 10mA 10W Start by F RUN 21 5Vh 500 V 10mA 5W Y SET 211 1000Vh RUN Ami 1000V 10mA 10w 900V 10mA 9W tC or wait 5 s 3 Y 4 RUN 2 448 880Vh 2500V 10mA 25W 5 END 2 4 3620Vh 51251 8mA 25W 1000 Vh pose PAUSE PI 1000 Vh 900V 25mA 9W ETE P SET aii 1000 Vh 1000 V 10mA 10W E Programming 2 4 3000 Vh 10mA 25W END 2500V Step Action 1 Choose Program number 2 Start the run run and make changes in the program 3 During a run you can view the settings It is also possible to interrupt the 4 Stop the run manually 5 The run is stopped automatically Figure 4 5 Running viewing and pausing a program View programmed values It is also possible to view the programmed values during a run by pressing set enter Note that no values can be changed here Only one phase is shown at one time Switch to another phase by using vA The display returns automatically to show RUN values after 5 s Alternatively use exit or run Electrophoresis Powe
12. run at either constant voltage current or power The EPS 3501 XL automatically switches over the controlling parameter according to programmed limits and conductivity variations in the system Thus the controlling parameter can switch within a phase Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 29 4 Operation 4 3 Setting up a run 30 Table 4 1 and Figure 4 2 illustrate a step program The programming and running of this application are shown as Figure 4 1 and Figure 4 5 E 3500 D 1500 Vh C 2000 1000 Vh A 1000 1000 Vh B 500 120 Vh 2 F No Description No Description A Phase 1 D Phase 4 B Phase 2 E Voltage V C Phase 3 F Time h Figure 4 2 Programming the voltage limiting profile in STEP mode The parameters shown are the same as those listed in Table 4 1 Table 4 1 The parameters of a step program Phase number Voltage V Current mA Power W Volthours Vh i 1000 10 10 1000 2 500 10 5 120 3 2000 20 15 1000 4 3500 30 25 1500 Choosing gradient means that a voltage endpoint for the actual phase is programmed together with current and power limiting values A linear voltage gradient is made with zero for the first phase or the programmed endpoint of the phase before for the next phases as starting point and the programmed endpoint as Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639
13. GE Healthcare Electrophoresis Power Supply EPS 3501 XL Operating Instructions Original instructions A e Table of Contents Table of Contents EE oos pee eR Re 5 11 Important user information nnne 5 12 Regulatory information n LS IMSTUMEN eniran e a metere ani 2 Safety TASER CTIOING sage ichcsacciandicesictesicsdecieiatelcestaediocasatatioscnaics 15 Safety precautioris sassacascarsnecscecocassecsansivascessvstsneasstoescancedearsescgunecieciauveutizastoiee 15 2 2 Labels ss a 18 2 3 Emergency procedures 2 4 Built in safety features 20 2 5 Recycling information e eieeeetnttnnnnnnnnntett 21 2 do een eee b t bd iem edt 23 3 1 Site requirements DAC 3 2 UNPACKING uala 3 5 ITObSDORL iconic een cae a NER ER dabit 23 544 CONNECCION S cccszcasscccsecadesioscyssvassssseatey seca a n AG i 24 3 5 Spare parts and accessories et ttttttttcnctna 25 4 ODOFUEHOD anui terrrertecrene rear rte cpeerstcrererte terete 27 4 1 Operation OVerview waeceecscsccsssssseesssssccssssssneesssescessssnmesesseseessssnusssseessssnnmeseseeseeeee 27 42 Starting the INSTRUMENT cs uu seated titles tenerte 27 4 5 Setting Up ATUN oe 28 44 Preparations before start 12335 45 BerformYgi a FU ice te HORREA OST E eei 55 5 Maintenance 51 General isse better titre bte a ri orent 6 TreubleshogDBigu dopo recie ipse aa ai bees 43 7 Reference information ees
14. Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 15 2 Safety instructions 2 1 Safety precautions WARNING Do not use EPS 3501 XL if it is not working properly nor if it have suffered any damage for example e damage to the power cord or its plug e damage caused by dropping the equipment e damage caused by splashing liquid onto items not supplied or recommended by GE Healthcare WARNING Operation and user maintenance of EPS 3501 XL should be performed by properly trained personnel only WARNING EPS 3501 XL is a high voltage instrument that can cause fatal electrical shock if the safety features are disabled The safety lid must be securely closed before starting a protocol WARNING The instrument covers must not be opened by the user It contains electrical circuits which can give a lethal electric shock Service and planned maintenance should be performed by personnel authorized by GE Healthcare PPP gt Personal protection WARNING Always use appropriate personal protective equipment during operation and maintenance of EPS 3501 XL gt Installing and moving the instrument WARNING Supply voltage Make sure that the supply voltage at the wall outlet corresponds to the marking on the instrument before connecting the power cord gt 16 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Safety instructions 2 Safe
15. alling using or maintaining the instrument Do not operate EPS 3501 XL in any other way than described in the user documentation If you do you may be exposed to hazards that can lead to personal injury and you may cause damage to the equipment Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 5 1 Introduction 11 Important user information Intended use EPS 3501 XL is a high quality high precision power supply for electrophoresis applications that require advanced programming and high voltage EPS 3501 XL is intended for research use only and shall not be used in any clinical procedures or for diagnostic purposes Safety notices These Operating Instructions contain WARNINGS CAUTIONS and NOTICES concerning the use of the product with meanings as defined below WARNING WARNING indicates a hazardous situation which if not avoided could result in death or serious injury It is important not to proceed until all stated conditions are met and clearly understood CAUTION CAUTION indicates a hazardous situation which if not avoided could result in minor or moderate injury It is important not to proceed until all stated conditions are met and clearly understood NOTICE o NOTICE indicates instructions that must be followed to avoid damage to the product or other equipment 6 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Introduction 1
16. ame regardless of the number of units The current should also be doubled if two gels are run on the same unit 4 5 Performing a run Running a program is described schematically in Figure 4 5 Choosing a program Press set enter and select the program you wish to run by pressing v until the value is correct Omit this step if you have just programmed or edited a method as described in Programming a method on page 28 and Editing a program on page 33 Running Press run to start the electrophoresis Information about the status of the start current check will be displayed for a few seconds The display will then show current values for voltage current and power and one of elapsed time volthours or milliamperehours You can switch between showing the elapsed time volthours or milliamperehours by vA The parameter controlling the electrophoresis is underlined A light emitting diode shows when voltage is applied HV on If no current is displayed or if HALT Low start current is shown check the electrical connections to the electrophoresis equipment Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 35 4 Operation 4 5 Performing a run 1 uz 1Vh 1mA 5W Confirm by 2 SET step GRADIENT Confirm by 2 5 SET 211 1000 Vh 1000V 120mA 10W Select by Mg Enter by 4 Insert INS del Select by A0 Confirm by Insert Delete 5a 212 1000 Vh 5b 212 00 00 h OW 20 mA 15W Programming po
17. below shows an example of the identification label that is attached to EPS 3501 XL XX XXXX XX EPS 3501 XL Code No X XXXXXXXX Voltage Serial No XXXXXXX Frequency Mfg Year 2009 Power max Fuse AN B ap C Cc LLL LU Made in Sweden GE Healthcare Bio Sciences AB en EON 751 84 Uppsala Sweden 26 Front panel labels Symbols used in safety labels Label Meaning Warning Read the user documentation before using the system Do not open any covers or replace parts unless specifically stated in the user documentation 18 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Label Safety instructions 2 Labels 2 2 d Meaning Indicates that hazardous voltages are generated inside the instrument The system complies with the requirements for electromagnetic compliance EMC in Australia and New Zealand CE The system complies with applicable European directives Labels concerning hazardous substances Label E Meaning This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment e This symbol indicates that the product contains hazardous materials in excess of the limits established by the Chines
18. e continued after pressing STOP Press run to run the same method again or press set enter to choose another program program a new method or make changes in an existing method Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Key Insert delete Label insert delete Introduction 1 Instrument 1 3 Description Press insert delete to insert or delete a phase in a program This function is activated in SET mode Note that a program must be completed by answering Ves Y to the question Last Phase in SET mode or by pressing exit before you can use the insert delete key more more Press more to place program in MORE mode This gives access to some special functions These include COPY Copying a program CLEAR Clearing a program SETUP Disabling the start current check MORE mode cannot be activated in RUN or PAUSE mode Leave MORE by pressing exit exit Press exit to stop the execution of an operation such as the entry of a value Only values units that have already been confirmed by set enter are retained when exit is pressed Note that if a phase contains zeros when pressing exit that phase will be deleted Press exit to return the instrument to the mode that was left or to the start position in SET Output sockets There are two sets of output sockets to allow two electrophoresis units to be connected and run at the same time O O O
19. e 45 1 SpecifiGaliong eater Reate aeter pp Pac G 45 T22 WENO Te saces acta Sectecgectas a Enna 45 735 Ordering infortridtiOri seii stleret iter betta tiges lide 45 Appendix A Electrical and communication connections 47 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 3 Table of Contents Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Introduction 1 Important user information 1 1 1 Introduction Purpose of the Operating Instructions The Operating Instructions provide you with the instructions needed to handle Electrophoresis Power Supply EPS 3501 XL in a safe way Prerequisites In order to operate EPS 3501 XL safely and according to the intended purpose the following prerequisites must be met e You should be acquainted with the use of general laboratory equipment and with handling of biological materials e You should understand the concepts of electrophoresis e You must read the Safety Instructions in Chapter 2 of these Operating Instructions e The system should be installed according to the instructions in Chapter 3 of these Operating Instructions In this chapter This chapter contains important user information and a general description of EPS 3501 XL and its intended use 1 1 Important user information Read this before using EPS 3501 XL All users must read the Safety Instructions in Chapter 2 of these Operating Instructions before inst
20. e standard SJ T11363 2006 Requirements for Concentration Limits for Certain Hazardous Substances in Electronics Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 19 2 Safety instructions 2 5 Emergency procedures 2 5 Emergency procedures This section describes how to do an emergency shutdown of EPS 3501 XL The section also describes the result in the event of power failure Emergency procedures In an emergency situation do as follows to stop the run Step Action il Switch off power to the instrument by pressing the Power switch to the 0 position If required disconnect the mains power cord The run is interrupted immediately Power failure The result of a power failure depends on which unit that is affected Power failure to will result in EPS 3501 XL The run is interrupted immediately in an undefined state 2 4 Built in safety features The power supply has the following built in safety functions Function Description Functional earth Should the power supply be connected to an electrophoresis leakage unit that has a leakage path to earth the EPS 3501 XL will detect this fault and turn off the high voltage Start current check The power supply checks that the resistance is not higher than a specified limit at a low safety voltage lt 40V If this resistance is too high the high voltage is turned off Too high resistance can be caused by for example
21. eaning agent Let the instrument dry completely before use WARNING Electrical shock hazard All repairs should be done by service personnel authorized by GE Healthcare Do not open any covers or replace parts unless specifically stated in the user documentation Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 41 5 Maintenance 5 1 General 42 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 6 Troubleshooting Troubleshooting 6 If an error that can be corrected by the user occurs either during a run or when switching on the power supply the program enters the HALT mode and the output is switched off Four different types of errors can cause HALT The following table shows the error message on the display the cause and the actions necessary Error message HALT Low start current Cause The current is less than the lower limit This can be due to incorrect connection of the electrophoresis equipment or due to use of buffers with extremely low conductivity Action 1 Check connections and or buffers 2 Press more switch off the start current check in the MORE mode See Electrophoresis Power Supply EPS 3501 XL User Manual for more information HALT Ground leakage current The current to ground leakage in the electrophoresis unit is too high Check the electrophoresis unit HALT Mains Voltage too l
22. en Introduction 1 Instrument 1 3 Description The HV ON lamp indicates that the high voltage power supply is on Power switch Switches on off power to the instrument Located on the rear panel Front panel The front panel consists of an alphanumeric display a keyboard with 9 membrane keys a light emitting diode LED that lights when voltage is applied HV on and connectors for two electrophoresis units Display 8 7 6 5 dL SET 171 0 20h 1600U 14inR 84i LN Part Description Part Description 1 Mode 5 Breakpoint parameter 2 Voltage 6 Phase number 3 Current 7 Control mode step 4 Power 8 Program number Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 11 1 1 5 12 Introduction Instrument Keyboard Key enter Label set enter Description Press set enter to enter a value or choice confirm this if valid and move programming to the next field Valid values are voltage 35 3 500 V current 1 400 mA power 1 200 W time 0 01 500 h volthours 1 500 000 Vh milliampere hours 1 25 000 mAh In the RUN mode pressing set enter shows the programmed parameters for the actual run In addition set enter allows you to make changes in the program during a run after first pressing pause continue After a run when in END mode pressing set enter puts the instrument into SET its programming mode
23. g information visit www gelifesciences com 2DE Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 45 7 Reference information 7 5 Ordering information 46 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Electrical and communication connections Appendix A AppendixA Electrical and communication connections Voltage 100 120 220 240 AC 37 e Frequency 50 60 Hz Power 260W AMox CD C 3 2 C C J b bicomectteforesevcng C gt lt j C X c gt 0 0 c gt x gt 7 B 0 Ti O 220 240 c 2 2 U y MORET B C PE 2 a C0 9 NS Part Description 1 Power switch 2 Power inlet 3 Voltage range switch Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 47 Appendix A Electrical and communication connections 48 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA For local office contact info rmation visit GE imagination at work and GE monogram are trademarks of General 7 Electric Company www gelifesciences com contact r CleanGel ExcelGal and Immobiline are trademarks of GE Healthcare companies GE Healthcare Bio Sciences AB All third party trademarks are the property of their respective owners Bj rkgata n 50
24. h the letter E or by the earth symbol or colored green or green and yellow e The blue wire must be connected to the terminal which is marked with the letter N or colored black e The brown wire must be connected to the terminal which is marked with the letter L or colored red Note After replacing or changing a fuse the fuse cover in the plug must be replaced with a fuse cover which corresponds to the colour of the insert in the base of the plug orthe word that is embossed on the base ofthe plug and the appliance must not be used without a fuse cover Note Only 15 Amps fuse approved to B S 1362 A S T A should be used 5 5 Spare parts and accessories For correct up to date information on spare parts and accessories visit www gelifesciences com 2DE Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 25 3 Installation 3 5 Spare parts and accessories 26 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Operation 4 Operation overview 4 1 4 Operation 4 1 Operation overview The operation of the EPS 3501 XL is described in the following sequence 1 Programming or editing a method 2 Editing a method 3 Running a method Programming and running are discussed in more detail in the following sections Blinking characters in the display are shown as bold characters in this manual 4 2 Starting the instrument Turn on the instrument by pressing the Power switch to the
25. he next phase After answering Y or N for the last phase the start position in the SET mode will be shown If alarm is selected for a phase a small A appears on the right of the phase number i e am Back to start position The program is now back to the start position in the SET mode with the program number flashing It is possible to go back to this position at any stage during programming in SET mode by pressing exit or STOP Note that the program is automatically saved with all choices that have been confirmed by set enter when exit or STOP is pressed If a phase containing invalid values zeros is left this phase will be deleted Disabling the start current check See Optional programming on page 34 if you want to use this feature Editing a program Changing a parameter value To change a parameter value move to the start position in SET mode by pressing exit or set enter and choose program number as described in Choosing a program on page 28 If the programming mode step or gradient is changed it will be changed for all the phases within the program Select phase number by w A and move in the program with set enter to the value to be changed Change with vA and confirm with set enter Press exit Inserting and deleting a phase Inserting and deleting a phase is described schematically in Figure 4 4 Note that you must first enter a phase before it can be inserted or deleted 1 If needed change program number by entering
26. int 4 Step Action Choose Program number 2 Choose STEP or GRADIENT mode 3 Choose Phase number Press Insert delete 4 Choose Insert or Delete 5a Program a new phase 2 5b Back to new Phase 2 start position Old phase 2 is deleted Figure 4 4 Inserting and deleting a phase in a program 36 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Operation 4 Performing a run 4 5 Pausing You can interrupt the electrophoresis for sample loading and or changing the program by pressing pause continue Voltage will no longer be supplied the HV on LED goes off and you may safely load your samples The display shows the status of the run when pause continue was pressed Switch between time integrated voltage and integrated current for the phase running when pause continue was pressed with v When sample loading is complete press either pause continue again or run to continue the run from where it was interrupted Editing a running program when in PAUSE When in the PAUSE mode you can also press set enter to make changes in the program This mode is called the P SET mode When the P SET mode is entered you can make changes as described in Changing a parameter value on page 33 It is not possible to insert or delete a phase for a running program The P SET mode is the same as the SET mode apart from restrictions in setting the breakpoint Naturally it is not possible to enter
27. ockets on the EPS 3501 XL front panel red to red and black or blue to blue see Section 1 3 The red lead is the positive and black or blue is the negative Or O O Electrical power 24 Connect the power cord to a grounded power outlet specified in Section 3 1 Select the appropriate voltage range 100 120 or 220 240 V using the switch on the rear panel NOTICE If the power supply is connected to 220 240 V with the range set to 100 120 V the instrument can be severely damaged Local regulation for Great Britain This appliance must be earthed The wires in the mains lead are colored in accordance with the following code Color Meaning Green and yellow Earth Blue Neutral Brown Live If the plug provided is unsuitable for your socket outlets the plug must be cut off and suitable plug fitted The cut off plug should be disposed of and must not be inserted into any 13 amp socket as this can result in electric shock The plug or adapter of the distribution panel should be provided with 13 amps fuse As the colors of the wires in the mains lead of this appliance may not correspond with colored markings identifying the terminals in your plug proceed as follows Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Installation 3 Spare parts and accessories 3 5 e The green and yellow wire must be connected to the terminal in the plug which is marked wit
28. ormation concerning the decommissioning of equipment Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 21 2 Safety instructions 2 5 Recycling information 22 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Installation 3 Site requirements 3 1 3 Installation EPS 3501 XL is delivered in protective packing material and shall be unpacked with great care Any equipment connected to EPS 3501 XL must fulfill applicable standards and local regulations For detailed information on Installation see Electrophoresis Power Supply EPS 3501 XL User Manual 3 1 Site requirements Parameter Requirement Electrical power 100 240 V 50 60 Hz Ambient temperature 4 C to 40 C Placement Stable laboratory bench Humidity 0 to 95 non condensing 3 2 Unpacking e Check the equipment for damage before starting assembly and installation Document any damage and contact your local GE Healthcare representative Remove straps and packing material and stand equipment upright before starting installation 5 5 Transport Before moving the system disconnect all connected cables Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 23 3 Installation 3 4 Connections 3 4 Connections Communication Connect EPS 3501 XL according to the electrical drawings in Appendix A Connect the leads from the electrophoresis unit to the output s
29. ow The mains voltage is too low refer to Electrophoresis Power Supply EPS 3501 XL User Manual Check voltage selector Check mains voltage HALT Mains fail Program stopped Mains power failure for more than 7 secs Press pause continue to continue running a program or STOP to interrupt the run If a serious error occurs the program enters the FAIL mode The output is switched off and an error message is shown in the display FAlL Code No xxx Call service Please note the error code number and contact your GE Healthcare representative Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 43 6 Troubleshooting 44 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Reference information 7 Specifications 7 1 7 Reference information 7 1 Specifications Parameter Value Ingression protection IP20 Supply voltage 100 240 V 50 to 60 Hz Power consumption Max 260 W Dimensions H x W x D 95 x 250 x 315 mm Weight 3 8 kg Ambient temperature 4 to 40 C Relative humidity 0 to 95 tolerance Atmospheric pressure 84 to 106 kPa 840 to 1060 mbar Acoustic noise level lt 70dBA 7 2 Literature For further information regarding Electrophoresis Power Supply EPS 3501 XL refer to the Electrophoresis Power Supply EPS 3501 XL User Manual 7 5 Ordering information For orderin
30. r Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Operation 4 Performing a run 4 5 Stopping the run and viewing end parameter values When the programmed time volthours or milliamperehours for the last phase is attained the program will enter the END mode It is also possible to break the run manually by pressing STOP In both cases the voltage current and power will go to zero as indicated by the HV on LED switching off The end parameter values are displayed Switch between total elapsed time integrated voltage or integrated current for all phases in the program by vA An alarm will sound at the end of each phase if selected in the program You can stop the alarm after the last phase by pressing STOP A run cannot be continued after pressing STOP Disconnect the leads and proceed with post electrophoretic techniques Since diffusion will begin as soon as the voltage is turned off you should remove the gel and begin staining blotting or autoradiography immediately Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 39 4 Operation 4 5 Performing a run 40 Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Maintenance 5 General 5 1 5 Maintenance 5 1 General No user maintenance is required for Electrophoresis Power Supply EPS 3501 XL NOTICE Cleaning Keep the instrument dry and clean Wipe regularly with a soft damp tissue and if necessary a mild cl
31. s in Figure 4 1 If h Vh or mAh is chosen the display will flash for the break value for the actual phase Set the value with w A and confirm with set enter Programmable values for time are 0 01 500 h volthours 1 500 000 Vh milliamperehours 1 25 000 mAh If OFF is chosen you have to break the electrophoresis manually by pressing STOP and only one phase can be entered The program will go back to the start position and the program number will flash Last Phase After programming the breakpoint you are asked if this is the last phase or not Select Yes Y or No N with vA and confirm with set enter Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Operation 4 Setting uparun 4 3 If Y is selected no more phases will be added and the alarm question will be shown see below If N is selected the next phase number for the program will be shown on the display together with zero values for all parameters Program the next phase according to Setting voltage current and power on page 32 and Figure 4 1 step 4 Up to nine phases can be programmed Choosing alarm The alarm can be set separately for each phase The following question is shown Alarm Phase 1 Select Ves Y or No IN with v and confirm with set enter If Y is selected in a 4 phase program this will be indicated by changing from to 1 after Phase on the lower row in the display Then you will be asked about an alarm for t
32. s not possible to insert or delete a phase for a running program Editing a running program See Section 4 5 Copying and clearing a program See Optional programming on page 34 Optional programming MORE mode contains three special functions e COPY Copying a program e CLEAR Clearing a program e SETUP Disabling the start current check For more information regarding these functions refer to Electrophoresis Power Supply EPS 3501 XL User Manual Choosing run parameters 34 EPS 3501 XL is an automatic cross over power supply that allows the user to set limits for voltage current and power It is also possible to program linear voltage gradients During electrophoresis only one of the parameters is limiting at a time The limiting parameter determines together with the conductivity in the electrophoresis system the values for the other two parameters For information about selecting parameter values refer to Electrophoresis Power Supply EPS 3501 XL User Manual Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Operation 4 Preparations before start 4 4 4 4 Preparations before start Connect the leads from the electrophoresis unit red to red and black or blue to blue Redis positive and black or blue negative Up to two electrophoresis units can run at the same voltage at one time Remember to double the maximum current and power conditions if two units are to be run Voltage will be the s
33. ssions and immunity requirements for electrical equipment measurement control and laboratory use Harmonized with 2004 108 EC 12100 2 EN ISO 12100 1 Safety of machinery Basic concepts general principles and design Harmonized with 2006 42 EC 14121 2 EN ISO 14121 1 Safety of machinery Principles of risk assessment Harmonized with 2006 42 EC CE marking The CE marking and the corresponding Declaration of Conformity is valid for the instrument when it is e used as a stand alone unit or e connected to other CE marked instruments or e connected to other products recommended or described in the user documentation and e used in the same state as it was delivered from GE Healthcare except for alterations described in the user documentation or explicitly authorized by GE Healthcare Regulatory compliance of connected equipment Any equipment connected to EPS 3501 XL should meet the safety requirements of EN 61010 1 1EC61010 1 or relevant harmonized standards Within the European Union connected equipment must be CE marked Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA Introduction 1 Instrument 1 3 1 3 Instrument This chapter contains a general description of EPS 3501 XL General description EPS 3501 XL is a high quality high precision power supply for electrophoresis applications that require advanced programming and high voltage
34. the start position in SET mode with set enter or exit changing the number with vA and confirming by set enter 2 Bypass the mode question with set enter Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 33 4 Operation 4 3 Setting up a run 3 Change to the desired phase number by vA and confirm with set enter Choose to delete the phase shown on the display or insert a new phase with this phase number by pressing vA and confirm with set enter If Delete is chosen the selected phase will be deleted and the program will move back to the position with the phase number blinking Note that by deleting a phase the numbers of the following phases will decrease by 1 If Insert is chosen the program will enter the same position and the new phase can be programmed as a new phase see Programming a method on page 28 and Figure 4 1 steps 4 8 After entering the breakpoint the question Last Phase will be bypassed and the starting point for the next phase will be entered Note that by inserting a new phase the number for the old phase with that number and the numbers for the following phases will increase by 1 Adding a phase after the last phase is done by entering the last phase as above moving to the question Last Phase and answering No N The program will jump to the start position for programming a phase with the phase number blinking Proceed with programming as described for a new program It i
35. ty precautions 2 1 WARNING Protective ground The EPS 3501 XL must always be connected to a grounded power outlet WARNING Power cord Only use power cords delivered or approved by GE Healthcare PPP WARNING Access to power switch and power cord Do not block the rear panel of the instrument The Power switch must always be easy to access The power cord must always be easy to disconnect System operation WARNING Hazardous biological agents during run When using hazardous biological agents flush the entire system tubing with bacteriostatic solution followed by a neutral buffer and finally distilled water before service and maintenance WARNING Avoid spilling liquids on the body of the instrument If large volumes of liquid have penetrated the casing of the instrument and come into contact with the electrical components immediately switch off the instrument and contact an authorized service technician Maintenance gt Pb WARNING Do not remove the main cover There are no user serviceable components inside and you can be exposed to high voltage Electrophoresis Power Supply EPS 3501 XL Operating Instructions 28 9639 69 AA 17 2 Safety instructions 2 2 Labels 2 2 Labels This section describes the safety labels and labels concerning hazardous substances that are attached to EPS 3501 XL Labels on the instrument Rear panel labels The illustration
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