LifeTouch User Manual
Contents
1. 1240 7210 00 20 01 00 00 05 Back SEW vant Finished jump setting 4 1 4 Extended parameters You can enter extended temperature li extended time speed and gradient and other operations by clicking extended parameters Extended temperature temperature increment per cycle Extended time time increment per cycle opeed heating and cooling speed Gradient temperature gradient refers to the tempearture difference among the most left column and the most right column of the module The temperature of the most left column is set in the temperature Ext temp Exttemp Ext time Rate Ext temp I belle I Lelie 4 Grade z lt te Ls m Back BED 2 x Cance Ext time Ext temp Ext time Ext 5 D HF el lef N B Rate Ol te E Le 2 Q vek Grade All view GG Back x Cance Rate Exttemp Ext time Rate O E el BD s Rate 4 te E Grade All view Le x B Gir Back x Cance Grade Exttemp Exttme Rate Grade Allview Temp EE Grade RN K i 072 0 9729 0720 072 0 072 0 072 0 egg 2 072 0 072 0 072 0 Instruction Manual of LifeTouch Thermal Cycler Display all browse the current setting of extended parameters and gt means to move the cursor left or right means to alter negat2. M Instruction Manual of LifeTouch Thermal Cycler 4 3 Set system parameters Click system setting in the main menu to enter main menu of system parameter setting Click touch screen to enter corresponding system setting interface 4 3 1 Date amp Time Click date amp time amp on the main menu of system setting to enter date amp time setting interface Click OK to save after setting 4 3 2 Language Click language DI on the main menu of system setting to enter language setting interface The instrument has integrated two languages namely Chinese and English Click OK to save after setting o K Heat cover Date amp Time Language Backlight D ES Beep Touch screen USB Main Main menu of system setting Date amp Time te BE AE itt B Back Ee Jes D x Cancel Date amp time setting interface Language Px English x Cancel Language setting interface 4 3 3 Backlight adjustment Click backlight adjustment YW on the main menu of system setting to enter backlight adjustment setting interface Press the navigation bar to move right and left to adjust backlight brightness of the instrument Click OK to save after setting Backlight adjustment setting interface 4 3 4 Hot lid setting Click hot lid setting I on the main menu of system setting to enter hot lid setting interface Clic
3. COM Port Settings xj Port Number 1 Part s Selected 1st port is Port 2 Basic Settings Advanced Settings Serial Parameters Tx Mode FIFO Enable v Fast Flush Only Flush Local Buffer Apply All Selected Parts Jf OK X Cancel COM port setting 6 3 Software upgrading First continuously press the touch screen and turn on the instrument power Starting up to enter upgrading status Then run PC software ThermalCycler Click tool firmware upgrading to select lower computer software bin that needs in upgrading Select upgraded object and communication mode and instrument address and click upgrading to start upgrading After finishing upgrading the equipment can be normally operated after restarting CON Port Settings Pot Number 1 Porfs Selected 1st post is Pet 7 Basic Settings Advanced Setting Serial Parameter COM Number COM3 curent assigned Firmware upgrade Upgrade bin file Serial Settings i 1 Portz Selected 1st port is Port 1 C Apply port alias to all selected port Port Alias Baud Rate RTS CTS Parity FIFO Enable Data Bits 8 Interface R5 232 Stop Bits 1 Flow Control Browser Upgrade target Upgrade single instrument Communication method Instrument address C Main file C Skin file Upgrade offline instruments xi 32 33
4. Click the Modify check box to modify configuration Jf OK x Cancel IP address setting 2 Click Operating Mode and select Modify Operating Node Click Settings and operating mode will POP for 1 Port s Selected 1st port is Port 1 selection Select TCP Server Mode and select Operating Mode TCP Server Mode de TCP Server Mode OK TCP Server TCP Client Mode UDP Mode TCP Server Mode Settings Miscellaneous Optional Local TCP Port 4001 TCP Alive Check Timeout Zo 0 33 min Inactivity Timeout Max Connection 0 0 655535 ms Data Packing Optional Delimiter 1 Off Hex Delimiter 2 Off Hex Force Tx Timeout 0 0 55535 ms Jf OK X Cancel Operating Mode setting 3 Click Serial option and set in the Serial Settings Serial Settings window that pops The specific parameters are as follows Click OK after finishing setting 1 Portis Selected 1st port is Port 1 Port Alias Serial Setting 4 Click OK to finish Configuration setting COM Port Settings 4 Click COM Mapping and Add target 9 to add target instrument Carry out COM port Pot Number 1 Pets Selected 1st post is Pott 1 settings in the pop up dialog box Click OK after finishing Click Apply Change ff to save Basic Settings Advanced Settings Serial Parameters altered setting COM Number COM3 curent assigned COM port settings 23 Ins
5. gt ER Network b EZ Control Panel 8 Recycle Bin D GeneTouchSetup 20110809 newpcrl10627 5 4 3 Equipment management Include connection operation connection and disconnection and instrument management searching for instrument adding instrument editing instrument removing instrument and showing all instruments mg ON E UN onne I mest Search Add Edit Remove All instrument instrument instrument instrument instrument Connect operation Instrument manage 26 Instruction Manual of LifeTouch Thermal Cycler 1 Connect Select equipment name sub item in the equipment list and click connect amp or right click connect equipment in the menu H will show 3 in connection show g in disconnection 2 Disconnect Select equipment name sub item in the equipment list and click disconnect S If the file or system parameters in the equipment are being edited then the dialog box will pop up prompt for save 3 Search for instrument Click search for instrument Q then a dialog box like the right figure will appear Search instrument eet 137 158 0 1 NEU 192 168 O 255 C Serial part Select added equipment and input equipment name Click Ole to save Set instrument name If the instrument needs to be connected please select Ge e _SerialNo Communication _Address automatically connect SN20410424 TCP 192 168 0 68 8N20110422 SerialPort COM3 8N20110423 SerialPor
6. If sound occurs or the display is abnormal or failure alarming and tone occurs during the process of the self test of the instrument after the instrument startup please cut off the power supply immediately and contact the supplier Caution If the sample quantity in your experiment is less than the number of taper holes of the module please try to distribute the sample test tubes evenly when placing tubes so as to ensure that the hot lid can be stably pressed on top of the test tube when running the file Meanwhile make the load of the module even to guarantee that the temperature changes of each test tube are uniform Caution Turn the knob in a counterclockwise way as required in knob figure press tightly in clockwise direction and release in counterclockwise direction before closing the lid until hearing twice clang sound Then turn the knob in a clockwise way before startup Turn another round after hearing twice clang sound Twice clang sound will be heard no matter turning the knob in a counterclockwise or clockwise way Unlock device knob can be released when pressing the unlock device and turning two rounds in a counterclockwise way simultaneously 4 1 Creating PCR program Click new file 2 in the main menu enter setting interface of PCR program 98 0 98 0 00 30 00 05 00 20 00 05 01 00 Ver 1 00 Block typle 96X0 2 BIGER lt 2 dae E BX 8 o Run NewFile System Seg Goto Delete Back Save Run
7. Instruction Manual of LifeTouch Thermal Cycler CHAPTER VII FAILURE ANALYSIS AND TROUBLESHOOTING This chapter mainly introduces possible failures of this instrument and reason analysis as well as troubleshooting 7 1 Failure Analysis and Troubleshooting S N Failure Reason Analysis Troubleshooting Power supply is not connected Check the power supply and connect 1 After the power supply switch is turned on Fuse is burnt Replace fuse 250V 8A 5x20 the display screen is not on and the toot ER sound is not heard either Switch is damaged Replace switch Others Contact the supplier or the manufacturer Module sensor is damaged or in Durina fi ETT poor contact sure Lari e AE E Heat sink sensor is damaged or in Contact the supplier or the 2 of Please Contact the Manufacturer poor contact manufacturer Hot lid sensor is damaged or in poor contact The ventilation hole is blocked Clear obstructions at the vent Open the case and check the wires Obvious changes in module heating rate S e Ge Gett 9 Wires get loose Tighten the screws of wire terminals if they get loose Cooling chip is damaged Contact the supplier or the manufacturer e SEN EE NEE Please properly adjust the ambient is so high that they exceed application M conditions of the instrument Ke Temperature cooling rate of the module 4 obviously slows down or it cannot slow Cooling chip is damaged down under room temp
8. address shall be set as 192 168 0 XXX Among them the range of XXX is 2 254 and cannot be the same with computer IP IP address setting 2 Click Operating Mode option and select Modify Click Settings then an operating mode for selection will pop up Select Real COM Moche or TCP Server Mode and click OK Operating Mode setting File Function Configuration View Help NW Network Enabler Administrator Configuration 222 eege Function Configuration 1 Network Enabler Module s Network Enabler Admit No Mode MAC Address IP Address Status 1 Configuration i w COM Mapping Big Monitor ill Port Monitor NE 41105 Message Log 1 Monitor Log 0 E EE gt e ec AC 1 2011 8 24 13 08 24 Found Network Enabler Module s 1 Now 2011 8 24 13 15 58 a Configuration Information Model NE 41105 v Modify IP Address MAC Address 00 90 E8 10 E2 B7 192 168 0 245 Auto Warning IP Address Report Password Digital IO Serial CMD Basic Network Serial Operating Mode Accessible IPs Serial Number 3838 Gateway Firmware Ver Ver 4 0 IP Configuration DNS Server 1 BIOS Ver Ver 2 1 DNS Server 2 299 299 299 0 Static Modify Status Data Mode omm unity Name Location Contact iv Enable SNMP public Click the Modify ch
9. class I IP20 SN KKKKKKKK_ KKK pate KKKK KK Made in China TECHNOLOGY CO LTD HANGZHOU BIOER b Warning Sign AN AN HOT SURFACE Warning The instrument is adhered with mark HOT SURFACE During operation of instrument or a period immediately after completion of running of a program any portion of body shall not be contacted with metallic portion module or hot lid nearby the said mark otherwise skin would be burnt 4 Maintenance of instrument In order to ensure full contact and good heat conduction between the test tube and the conical holes the conical hole on the module shall be regularly cleaned with clean soft cloth soaked with a little absolute alcohol The stained surface of this instrument if any shall be cleaned with soft cloth soaked with cleaning paste Warning During cleaning the instrument its power supply shall be cut off The instrument surface shall not be cleaned with corrosive cleaning agent 5 After sales service the warranty content and scope are shown in the warranty sheet Caution a After unpacking immediately check the goods against the packing list In case of damaged or missed goods please immediately contact the supplier After qualification of acceptance properly fill the product acceptance sheet and send or fax the copied sheet to the supplier for filing and maintenance After unpacking the packing box and packing materials shall be wel
10. not disassemble instrument without permission Only qualified professionals are allowed to replace elements or adjust the machine inside Replacement of elements under energizing state shall be prohibited c Use of power supply Before turning on AC power make the power voltage consistent with the instrument required one a 10 deviation is allowed Make the rated load for power socket not below instrument s maximum load d Power wire Generally this instrument uses accompanied power wire The damaged power wire shall be replaced by one power wire of the same type and the same specification It can t be repaired The power wire shall neither be compressed nor be laid in gathered personnel location e Insertion and withdrawal of power wire At insertion and withdrawal of power wire the plug shall be correctly held with hand At insertion the plug shall be completely and tightly inserted into the socket and at withdrawal heavily pulling the wire shall not be allowed f Placement of instrument This instrument shall be fixed in a low RH and low dust place apart from water source e g water pool and water pipeline and the room shall be well ventilated and free from corosive gas and interterence of strong magnetic field The instrument shall not be placed in a wet or dusty location The openings on this instrument are for ventilation circulation and in order to avoid over heat of the instrument body they shall not be blocked or covered When a s
11. 00 30 Q Hotid heat Lid temp Block temp d lg i d Program running interface TT TT TT 720 00 20 In the running process the user can click main menu to return to Eege 55 30 38 C 00 40 45 the main menu or carry out other operations Such as creating new file If you want to return to the program running interface again you can click the running interface at the top of the screen on the main menu Seg Lid temp Block temp Remain time Block typle 96X0 2 Main menu in program operation 4 2 2 Run saved PCR program 4 Recent 4 123456 4 7 p 4 Share 123T 4 7TTYREEYUT V G D 1 4 2 14 8 1 JK N EN NW W e D 7 1 1 10YRJRWY7432 IK 4 K G E W LA 1111 IH e H coccccccccce D K D 4 123 4 654321 4 J page 1of2 page 1 of 1 m p CH 1 r un CT WI Y A y Select User New User Delete User Rename Main Run New File Edit File Delete File Back Main Option interface for users Option interface for program file Under the main menu clicks run to enter Click corresponding user name to enter option interface option interface for users as the above for programs as the above figure In the option interface figure In the option interface for users the for programs the user can create new file edit file delete user can create new user delete user and file and run selected file etc rename etc 14
12. 2 503 504 Ext Temp 0 0 C Ext Temp 0 0 C Ext Temp 0 0 C Ext Temp 0 0 C Ext Time 00 00 Ext Time 00 00 Ext Time 00 00 Ext Time 00 00 Grad 0 Grad 0 Grad 0 Grad 0 100 C 80 60 C 40 20 4 1 4 0 C 4 0 40 4 0 C oc 00 10 C ggg IC open IC ggg 3 Alter password Select the file that needs to alter password and click alter password a Input old and new passwords in Change file password the pop up dialog box and click OK to save Do you want to change password of Computer gt test gt 11 If the selected file is not protected by password then Enter old password it only needs to input new password Enter new password EE The password can contain only letters and numbers 4 Rename Select the file that needs to be renamed in the Change file name equipment list and click rename j It cannot be re Do you want to change file name of Computer gt test gt 11 7 renamed when the file is opened Enter new file name Furre The file name can contain only letters and numbers 5 Delete file Select the file that needs to be deleted in the equipment list and click delete aol If the file is being edited then it cannot be deleted If the file is protected by password then it cannot be deleted unless inputting correct password 6 Copy Select the file that needs to be copied and click copy Ch or right click copy fi
13. 38 Run 3jp user Alec 2011 04511 18 42 38 Run CHAPTER VI FIRMWARE UPGRADING 6 1 Connect to PC Connect the equipment with computer by network cable Turn on the equipment power to make the equipment on standby Network cable Computer Instrument 6 2 Network Enable Administrator setting 1 Run Network Enable Administrator tool software When upgrading the instrument communicate with virtual device mapping used N Network Enabler Administrator Configuration Sela File Function Configuration View Help 2a2 9 Function Configuration 0 Network Enabler Module s 5 Network Enabler Admit No Mod X MAC Address IP Address Configuration dk COM Mapping Monitor Port Monitor XU IP Address Report Message Log 0 Monitor Log 0 No Time fDeseption 0 0 0 0 0 0 0 0 0 0 0 0 0 How 2008 1 3 14 15 25 30 31 Instruction Manual of LifeTouch Thermal Cycler 2 Click search button or right click Broadcast Search option in Configuration the tool will automatically search for the equipment 3 Double click the network number searched in list column to enter configuration setting page like the right figure 1 Click Network option and select Modify Input IP address and subnet mask The instrument IP address shall be in the same network segment with computer IP address For example if the computer IP is 192 168 0 1 then the instrument IP
14. J9 6 LAN pk Hv Ale 2MV 01L221C3 44 A Dis Ft END i LINE3 1 Ne erik J42 3 CS i 350mm J8 1 MOT1 PCB3 C EI 6 NI J42 4 DIR i U5 J8 2 MOT2 J6 1_24VFAN CPU 342 8 HV FAM RR TT ee BYQ507101200000 42 6 HV i E04SR150718 Am 4 MOT4 LINE4 150mm i J2 1 SW J26 BLK amp ZJB 40P J PLUS1 1 lt J2 2 SW FAN1 WAAN IN 77 EE J_PLUS1 6 J3 2_ON OFF i LINE3 J23 GND J5 CPU 40P J6 ZJB 34P LINE COSEL J i e K IAA 36V600W FF l T EFS SE k fife A l ARE SWITCH BLAR LAE Eo 165 Sidet 300mm R13 33A MR 4 210 C5 BB SL SE ADAGOOF 36 E LINE2 LINE2 l AC250V8A 5 20mm B 1 350mm L 3 350mm a FUSE FUSE L EE E A i gr Li E 160mm i Dr 350mm i 4 300mm LINE2 U LINE2 BAB A E T FJ FUSE FUSE N Ir FE rp addi ss RF187 8 LINE2 EEE Hr 300mm IZ NEN PRA ES 2 AE RAR FL 25 4 sek ETE MEN HAVEBY ht BLE ED RE BON 35 MEMORANDUM 36 Add Tel Fax E mail BO BIOER mad TECHNOLOGY 1192 Bin An Rd Hi tech Binjiang District Hangzhou 86 571 87774575 or 87774303 86 571 87772210 overseas bioer com cn 310053 lt I mem a i i Ssss Ee g 3 a9 2 ee ms mmer LITE iu NIE d li i l i Il ll b NM li i 890 S o
15. OGraM ME 5A 4A A 12 4 2 2 Run saved PCR e Jo z lA M Yt 14 4 3 Set system parameters Y 15 A cS Ei o oo oo mmm 15 Zo Sep Language EE 15 4 3 3 Backlight adjustment M ue 16 4 3 4 Hot lid setting 16 EC Na 16 NAN ECCL LLL S LL LS aiai EREET EEEE e ENEE a RA OA ia ERAON aa aE iaaa 16 Er ER RR EE EEE RE 17 4 4 Distribution characteristics of gradient module En 17 CHAPTER V CONNECT TO COMPUTER EE 18 5 1 System configuration nennen 18 52 instal sofware LLLI LLLI 18 FN AH 18 Seel eene 19 5 3 Online setting EE 20 FA EP r vere 20 EPSON PR VD 23 TINN JJ 23 5 4 2 File management Mee 24 ENN ee 26 5 4 4 Other management Mna 28 FN 29 CHAPTER VI FIRMWARE UPGRADING G etta itti ntes htt b ten iSi shed este tentes anra bet ek reni 30 61 Connect Ce A Ce 30 6 2 Network Enable Administrator setting NEEN 30 6 3 Software siele ollas ME 32 CHAPTER VII FAILURE ANALYSIS AND TROUBLESHOOTING EEN 33 7 1 Failure Analysis and Troubleshooting EA 33 Appendix 1 Wir
16. T O D Q zu TECHNOLOGY Thermal Cycler LifeTouch INSTRUCTION MANUAL tp gpa Ye ET i HINE Kar Eh av tinci i eh oe se H prener EE EAE gt kjem be m Oe HET EVEN areraer B 3s amp ar amp RM p ororo gegen ct ka 3 5 ESEE S D TENG e PER LUE pe app z piei e h HERE H wt uutta Doto2 guurta SSE EN o192 uut q anop edna cie 9 v JI UM S 14 Pr um vn MID M D TI wb P d I D dt dA hs a M will d jj en TC Caution The Bioer Co reserves the right to modify this manual at any time without notice The manual contains copyright protected and patented material Without prior written consent from Hangzhou Bioer Technology Co Ltd any part of the manual shall not be duplicated reproduced or translated into any other language Before initial use of this instrument please carefully read this manual Product Patent No ZL 20092019972313 Product Standard No YZB Z 0189 2010 The product is for scientific research only Document No BYQ607200000000ESM Document Version October2011 Version 1 4 Instruction Manual of LifeTouch Thermal Cycler IMPORTANT NOTES 1 General Practice Caution Important information is contained in any item and should be carefully read Failure to observe the instruction would result in damage or abnormal functions to instrument Warning The warning message requires ex
17. Touch Thermal Cycler LifeTouch Thermal Cycler is a PCR instrument with multiple functions and several applications Below are its features Use advanced thermoelectric refrigeration technology from ITI of US and thermoelectric refrigeration elements made under quality management of Japan which are steady and reliable in quality Temperature can be changed as fast as 4 C second The speed to increase or decrease temperature can be set as per need of experiment Several modes for temperature control better compatibility of reagent Large LCD screen user friendly operation interface simple and easy operation Power off protection available programs can continue after power recovery Height and pressure of hot lid can be set for different reaction tubes which effectively prevent the reagent from evaporation and pollution Connect to ordinary computer by RJ45 standard interface which is easy for software upgrading 1 4 Use of LifeTouch Thermal Cycler Integrating a number of advanced technologies Lifetouch Thermal Cycler meets the demand of different PCR experiments and ranks top in similar products LifeTouch Thermal Cycler is attached with 1 2 Strip of 8 tube 3 Strip of 12 tube 4 Full half apron plates 0 2 ml standard centrifugal tube Instruction Manual of LifeTouch Thermal Cycler CHAPTER II FEATURES This chapter introduces the usage transportation storage conditions of the instrument as
18. cur the supplier or manufacturer should be contacted timely 2 since different brands and all instruments have their own temperature control characteristics including heating and cooling rate stability and volatility and due to the uncertainty of biological experiment and external vulnerability successful PCR program effect in one instrument may not apply to another Therefore when you change the instrument please adjust PCR running program so as to achieve the ideal state ICE t to change without further not and is subjec For reference only iagram D iring W Instruction Manual of LifeTouch Thermal Cycler BIOER Appendix 1 Wu DERE BYQ507101310000 100mm T gt y e th oe LINES on Lt Geit i BSR PCB2 HK BYQ507101410000 TT S SSS Hi x 11 POW 34P i J5 1 TE1 Aus dal ED 1 J2 2 TE1 J7 CPU 40P 5 2 TE1 ARSE HE J2 3 TE2 J5 3 TE2 J PLUS1 2 J PLUS1 3 J PLUS1 4 J PLUS1 5 J23 SW AD1224HB F51 J3 1 SW T 7 S J24 POWBOARD_40P J6 1 USB VCC J3 2 SW DC CU J6 2 USB DM1 J3 3 SW J42 2 V J6 3 USB DP1 WI J42 1 V J6 4 USB GND DC J6 5 USB GND LZ J6 6_USB GND J2 4_TE2 J2 13_ 5V Fae J9 1 LAN J5 4 TE2 Ss i J2 14 GND J9 2 LAN UGenePro amp H T He p gw J4 1 V J9 3 LAN Ah elt id ER De EK d J4 2 V i J9 4 LAN J4 BLOCK 48P BYQ507101320000 110mm J1 2 RxDO J9 5 LAN BYQ5071020000004tEt WA e mx J42 1 3 3V i J1 3 TxDO
19. eck box to modify configuration Operating Hode 1 Part s Selected 1st port is Port 1 Operating Mode TCP Server Mode es E TCF Server Mode TCP Server Mode Settings Local TCP Port Max Connection Real COM Mode JS OK X Cancel TCP Client Mode UDP Mode Miscellaneous Optional TCP Alive Check Timeout 0 99 min Inactivity Timeout 0 65535 ms Data Packing Optional Delimiter 1 Delimiter 2 Force Tx Timeout Off Hex Off Hex D 0 55535 ms AAK X Cancel 3 Click Serial option and set in the pop up Serial Settings window The detailed parameters are as follows Click OK after finishing setting 4 Click OK to finish Configuration setting Serial Setting COM Fort Settings E Part Number 1 Fortis Selected 1st port is Port 2 Basic Settings Advanced Settings Seral Parameters Baud Rate Parity Data Bits Stop Bits Flaw Contral Mane 8 1 Mane Apply All Selected Parts af OK 3X Cancel Ei 4 Click COM Mapping and Add target to add target instrument Double click the added instrument in the list of COM Mapping COM Port Settings dialog box will appear Set COM port in Basic Settings change Mode to Classical in Advanced Settings set Band Rate to 38400 in Searial Parameters then click OK to finish settings Click Apply Change 9 to save changed setting
20. erature Contact the supplier or the manufacturer Fan is damaged or out of work 5 The temperature sensor is damaged Contact the supplier or the The module neither heats nor cools manufacturer Cooling chips are all damaged In system parameters setting interface Set the hot lid status at a certain the hot lid status is set as OFF temperature value Temperature control in file edition 6 Hot lid cannot heat interface is set as so that the Set the temperature time as number form hot lid automatically closes Connectors get loose A Hs ENEE Contact the supplier or the manufacturer Temperature sensor of the hot lid is damaged Chip is in poor connection 7 The screen shows abnormal characters Contact the supplier or the manufacturer Chip is broken S N Failure Reason Analysis Troubleshooting Have not set hot lid temperature Please refer to 4 3 in Chapter IV of the the hot lid is set as OFF Instruction on Setting Hot Lid Temperature Adjust hole location of the reaction tube 8 Reagent in reaction tube evaporates The reaction tube is unevenly placed try to place in a symmetrical way Please tighten the reaction tube The reaction tube is not tightened before placing it into the instrument Caution 1 The user is not allowed to open the housing of the THERMAL CYCLER for inspection by himself during the warranty period If any failures that require opening the housing for inspection oc
21. id setting the lowest temperature of hot lid and module retention temperature Clicks save after finishing setting The lowest hot lid temperature range OC 99 9 C Module retention temperature range OC 30 C The system information cannot be set when the instrument is disconnected When there is only single module in the connected instrument the display area of module B cannot be altered 5 4 4 Other management 1 lemperature gradient Click temperature gradient i in the menu bar and a gradient dialog box as follows will pop up Temperature gradient range temperature 0 C 690 C Gradient temperature 00 C 300 C Edit instrument Instrument address 182 158 O 47 For gt Setting W Sound Control Beep alarm when run finish Key been OFF v Complete Module A Lowest hotlid temperature C Hotlid will close when temperature lowes this Module keep temperature 20 M Module keep this temperature when hotlid temperature is rising up Module B Lowesthotlid temperature 30 0 C Hotlid will close when temperature lowes this Module keep temperature 10 M Module keep this temperature when hotlid temperature is rising up emperature Temperature 50 gt Gradient 20 gt 1 2 3 4 5 Z 8 39 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 Q0000000000 10000000 000000800892 A31 00600000 38 3 69 1 70 0 Close 28 29 Rea Instruction Manual of L
22. ifeTouch Thermal Cycler 2 System help Click help then the help file will appear 3 Software edition Click about the system dialog box will pop up and the software edition can be referred to 5 4 5 Run 1 Run file Open the file and click run k then a run file dialog box like the right figure will pop up Select instrument Do you want torin Tio Please select instrument or module and input parameters module test tube type amount of increased fluid CEE E temperature control mode hot lid temperature and doca EA 02m M ue M running method limited to Verit Module Click OK to NS run program Select all Unselect all v OK After running the file the running status on the Online v Offline right of the main menu shows like the right figure EE e GeneTouch 48D gt nd 27 3 C B Module temp 29 9 C Details of instrument operating status are as follows lical gt Fan TU x I T r ba Tube volume Module temp 5 C g Total time pera me es Bet Tia 00 00 Fee Tie 0200 Grad trad CG 2 Browse running history local gt History x Module Right click system history and a history table as name Username File name Begin time End time State follows will pop up A user002 file0021 2011 04 11 13 29 04 2011 04 11 13 31 20 UserSto 2 A user geng 2011 04 11 18 42
23. ing Diagram 35 Instruction Manual of LifeTouch Thermal Cycler CHAPTER I INTRODUCTION The contents in this chapter include introduction of PCR technology examples of PCR application introduction of Thermal Cycler and features of Lifetouch Thermal Cycler 1 1 Introduction of PCR technology Polymerase chain reaction PCR is a nucleic acid amplification that simulates natural DNA replication in vitro It takes 2 DNA chains to be amplified as template uses a pair of artificially synthesized oligonucleotide primer as mediator and amplifies certain DNA fragment quickly and specifically by thermostable DNA polymerase Ever since PCR technology was invented by Mullis in 1985 and thermostable DNA polymerase was discovered by Erlich in 1988 this simple fast specific and sensitive technology has been widely applied to life science areas at astonishing speed Remarkable achievements have been witnessed in cytology virology oncology genetic disease forensic medicine animal plant immunology and etc It is a milestone in contemporary molecular biology development 1 2 Example of PCR technology application scope Research Clinical diagnosis Immunology Human genome project Forensic medicine Oncology Histology population biology Paleontology Botany zoology Gene clone dna sequencing mutation analysis gene recombination a
24. ingle set or several sets of instrument are used the interval between its surrounding ventilation opening and its nearest object shall not be below 50cm or 100cm Also don t use the instrument on loose or soft surface or the air inlet at instrument bottom might be blocked Excessive temperature would impair the test performance or result in instrument failure This instrument shall not be used in location subjected to direct sunshine The instrument shall be kept away from hot gas stove and all other heat source If the instrument is to be set aside for a long time the power plug shall be withdrawn and the instrument covered with soft cloth or plastic film to aviod entrance from dust g Cautions during operation During test cares shall be taken to prevent liquid from dropping onto the instrument Caution In any of the following cases immediately cut off the power supply withdraw the power plug from the power socket contact the supplier or ask qualified repairman for treatment O Liquid drops into inside of the instrument The instrument is rained or watered The instrument works abnormally especially generates abnormal sound or odor The instrument is dropped or its casing is damaged The function of instrument is obviously changed 3 Instrument label a Label EC Name Base for LifeTouch Thermal Cycler Model TC 96 G H b B Power 100 240V 50 60HZ 600W Fuse AC250V 8A o 5x20 Sort
25. istrator Setup Wizard JL HP Setup has finished installing Network Enabler Administrator on 5 Pi n S h n stal lati O n C IC k Fi n S h b your computer The application may be launched by selecting the installed icons Click Finish to exit Setup 5 3 Online setting 5 3 1 Connect to PC Connect the equipment with the computer through network cable intersect connection B Network cable Computer Instrument 20 21 Instruction Manual of LifeTouch Thermal Cycler 1 Run Network Enable Administrator tool software 2 Click search buttons or right click Broadcast Search option in Configuration the tool will automatically search for the equipment 3 Double click the network number searched in list column to enter configuration setting page as follows 1 Click Network option and select Modify Input IP address and subnet mask The IP address of the instrument shall be in the same network segment with that of computer For example if the computer IP is 192 168 0 1 then the instrument IP shall be set as 192 168 0 XXX Among them the range of XXX is 2 254 and cannot be the same with computer IP Network Enabler Administrator Configuration File Function Configuration View Help Sa eg Function Configuration 0 Network Enabler Module s Eg Network Enabler Admit No Model MAC Address IP Address Configuration GG COM Mapping Monitor Por
26. ive and positive data Clr means to clear data 4 1 5 Save Run All view Exttemp Exttime Rate Grade All view 2 0 1 00 Ext temp 0 0 Ext time 00m00s Rate 4 0 s Grade 12 v OK lt gt ber YT Veg d Kl W D e Seg Goto Delete Back Save Run After setting PCR program click save run to enter save interface as shown in the right figure The user can input user name and file name in the interface and also can adopt password assess mechanism for the data Finally clicks save User Name File Name Password Password Confirm e L Iele Ls LsL Lele Lol lw D z Lv Ju fele leet Eau sj oj ref e of e i er zx ev e INIM sae Jf F V ZZ Save interface of PCR program file Ca under the user name by clicking icon as shown in the right E i UserName ME EZ figure FileName User Select Password 1 10 Password Co TR JRWY7432 Eng 123 eile Sr add OOC za mo 25 EI ci ST Ok x Cancel Jl jue jum Jum j Save t Main 4 2 Running PCR program 4 2 1 Run new PCR program 1 After saving the new PCR program click run to enter parameter setting as shown below The user can alter temperature control mode E D D Run Winzard increased amount of fluid and temperature of hot lid including opening closing hot lid function User TEST File 1 8 Sample Type 96x0 2 L5 6 Co
27. k OK to save after setting ot lid setting interface 4 3 5 Tone Click tone on the main menu of system setting to enter tone setting interface Click OK to save after setting Tone setting interface 4 3 6 Screen calibration Click screen calibration IP on the main menu of system setting to enter screen calibration setting screen In the setting process you can finish screen calibration function by clicking upper left corner of the screen and figure center at the lower right center in turn with touch pen as per the prompt Screen calibration interface Back light aa EE 9 7 ok Heat cover Close cover while module temp below Keep module temp When cover heating Ls BE el e te BD Beep Key beep Alam beep End beep x Cancel Please click the central point of the figure with a touch pen Please click the central point of the figure with a touch pen Instruction Manual of LifeTouch Thermal Cycler 4 3 7 USB Usbdik Usb disk Insert USB and click USB amp on the main menu of system setting The equipment will automatically search for U 1 Input file disk The user can export file to the U disk after successful searching Output file T9 USB search interface 4 4 Distribution characteristics of gradient module The gradient module adopts 2 temperature control units The temperature gradient distributes li
28. ke a curve See as the following figure set temperature at 40 C and temperature gradient at 20 C Each hole temperature gradient of the module do not distribute evenly Temperature difference of each hole in the middle of the module will be larger than that of holes on the two sides of the module Therefore when operating gradient module temperature of each hole shall be subject to the temperature displayed on the instrument Gradient graphs Temperature C GES S z 40 1 2 3 lt 9 6 T 8 9 10 11 12 Column left to right CHAPTER V CONNECT TO COMPUTER 5 1 System configuration Operating system System environment Operating environment Other software CPU Minimum configuration Memory Hard disc 5 2 Install software 5 2 1 Install PC software Windows XP Windows Vista Windows 7 Net Framework2 0 PDF reader Pentium R 4 1GB 10GB Click ThermalCyclerSetup exe in CD ROM to install Life Touch online program 30 Life Touch instruments can be controlled simultaneously by connecting to computer program 1 Click and run ThermalCycler Setup exe to select installation language and click OK 2 Select installation location of the software and click next 3 Click next n j5 Setup ThermalCycler Welcome to the ThermalCyder Setup Wizard This will install ThermalCyder version 1 0 on your computer It is recommended that you dose all other applications before con
29. l kept for future repairment For the damage of instrument occurring during transporting to the repair department due to improper package Hangzhou Bioer Technology Co Ltd shall bear no liability Instruction Manual of LifeTouch Thermal Cycler CONTENTS CHAPTER I INTRODUCTION EE 1 1 1 Introduction of PCR technology LLL LLLI 1 1 2 Example of PCR technology application SCOPE mmn 1 1 3 Features of LifeTouch Thermal Cycler M I AT 2 1 4 Use of LifeTouch Thermal Cycler Po 2 CHAPTER II FEATURES EE 3 2 1 Normal working conditions mmn 3 2 2 Transportation and storage conditions eene 3 SEITEN 3 24 Software functions EN RE EE 4 CHAPTER Ill PREPARATION EE 5 3 1 Structure diagram LLL 5 3 2 Examination before Startup EE 6 33 Startup 6 CHAPTER IV OPERATION GUIDELINE 7 4 1 Creating PCR sltelet CT 7 4 1 1 Add or delete program Segment eene nennen nnenennenneenne nnne nne enenennen nennen nnne 8 AT 2 Program and constant temperature ME 4 8 4 1 3 Cycle g S exe MET 9 4 1 4 Extended parameters HM 10 MI Ba E 11 42 Running PCR e CeTe CT 12 4 2 1 Run new PCR Pr
30. le in the menu to copy the file If the file is being edited then it cannot be copied 7 Paste Select the equipment option in the equipment list and click paste 75 or right click paste copied file in the menu to paste the program file under the user and the user name if the copied user has already existed then it will prompt whether to cover in pasting If the program file under the existed user name is being used then it cannot be pasted You can use mouse to drag and paste 8 Import file Click import 4 and select the folder of the user in the Qm ft Search Desto p EE Desktop gt NES 7 TT pop up window Click OK to import file If there is same file name then it will prompt whether to cover o in importing rmm ua mun If the file with the same name is in use then it cannot be uw BE ioun imported d Sr liis Pictures newpcr110627 B Videos File folder test ear u Fe Computer EAR File amp OS c 4 00 KB Filename test ear v Ear File ear x Browse For Folder 9 Export file Click export A and select the folder saved by the user in Please select export file s target path the pop up window Click OK to export file If there is same file name then it will prompt whether to cover in exporting If the file is being edited then it cannot be exported b Ag Libraries gt UA zhs p jE Computer
31. lecting the installed icons Click Finish to exit Setup i FIT NN ILLI Hnshigr Ldesoxzstraten Welcome to the Network Enabler Administrator Setup Wizard Thn vell stall Meier Era Adbrinzeh ghe 2 11 en peur Copies Bic miiia b t vena der all other a kstaer bel rorfruameg Dick ed Ba connue ex Cancel ba mal Setup a Setup Network Enabler Administrator Select Destination Location Where should Network Enabler amp dministrator be installed CJ Setup will install Network Enabler Administrator into the following folder To continue click Next If you would like to select a different folder click Browse C Program Files Network Enabler Administrator At least 2 5 MB of free disk space is required e Setup Network Enabler Administrator Select Additional T asks Which additional tasks should be performed Select the additional tasks you would like Setup to perform while installing Network 9 Select installation list and click Next Enabler Administrator then click Next Additional icons 2 Setup Network Enabler Administrator Installing Please wait while Setup installs Network Enabler Administrator on your computer T n Extracting files 4 G IC k n stal C Program Files Network Enabler Administrator NEAD MIN exe TTELEELEELLEL ELE ELE LEE LLL LLL Lacie eo g Setup Network Enabler Administrator Completing the Network Enabler Admin
32. n use the user z b e DVD Drive E cannot be imported gt Network gt EE Control Pan 7 Export user Click export to select the folder saved by the user Click OK to export user If there is same user name then it will prompt whether to cover in export If the program file under the user is being edited then the user cannot be exported 5 4 2 File management Include creating renaming deleting copying pasting importing and exporting user etc 7 da D el i al gi ES E m Save Change Rename Delete Copy Export password File operation 1 Create Select the user that needs to create file and click create Input file name and password and click OK Create file Do you want to create new file in test of Computer Enter new file name The file name can contain only letters and numbers Enter password The password can contain only letters and numbers EE 24 25 Instruction Manual of LifeTouch Thermal Cycler 2 Edit and save Select the file that needs to be edited and click edit or right click edit file Click save e in the menu bar after finishing edition SEG amp Ald Fs ei sl 3 opy in Export Create 1 Save iperation File operation Computer gt test gt 11 x Segment FAAdd C Copy F Delete Cycle QiAdd Delete Run 501 50
33. nd fusion protein regulating dna sequence Identification transposition insertion site mapping genetic modification detection synthetic gene construction clone construction carrier expression certain gene s incision enzyme diversity detection and etc Bacteria spirochete mycoplasma chlamydia mycobacterium rickettsia diphtheria bacillus pathogenic e Coli dysentery bacillus aeromonas hydrophilic and c Difficile and etc Virus htlv hiv hbv hev hpvs ev cmv ebv hsv measles virus rotavirus parvovirus b19 and etc Parasite malaria and etc Human genetic disease lesh nyhan syndrome mediterranean anemia hemophilia bmd dmd cystic fibrosis and etc HLA typing T cell receptor or antibody diversity qualification autoimmune disease gene mapping lymphokine quantification and etc DNA mark generated from scatter repetitive sequence genetic map construction detect DNA polymorphism sperm drawing physical map construction Sequencing expression map and etc Crime scene specimen analysis hla dqa analysis and etc Pancreas cancer rectal cancer lung cancer thyroid cancer melanin cancer hematological malignant tumor and etc Genetic cluster research evolution research animal protection research ecology environmental science experimental genetics and etc Archeology and museum specimen analysis and etc Animal inflectional disease diagnosis plant pathogen detection and etc 1 3 Features of Life
34. ntrol Mode 7 Tube Block elo Sample Volume Hotlid Control v On P ds n Parameter setting interface Instruction Manual of LifeTouch Thermal Cycler The mode Block only takes into account of the temperature control in rise and fall of the module Since the rise and fall of the reagent lags behind the module to certain extent the actual temperature change process of the reagent is different from the program we set The actual time during which the reagent reaches the setting temperature will be far less than the setting time see the relationship between module temperature and reagent temperature in the right figure pt tt tT HE P V Temperature C Tube mode takes into account of the backward process during which the rise and fall of the reagent lags behind the module After reaching the setting temperature there will be an overshooting process so that the reagent can reach the setting temperature at a quicker speed In the setting time that similar to that of Block mode the actual time during which the reagent achieves the setting temperature evidently prolongs see the relationship between module temperature and reagent temperature in the right figure Mel TTT NUT TT T TAKT Temperature C 2 After setting temperature control mode increased amount of fluid and temperature of hot lid click OK to run PCR program J610 os 98 0
35. numbers 3 Delete user Select the user needing to be deleted in the equipment list and click delete or right click delete user to delete The program file under the user name will be simultaneously deleted when deleting the user 4 Copy Select the user needing to be copied in the equipment list and click copy a9 right click copy user in the menu to copy the user and the program file under the user name It cannot be copied when the program file under the user is being edited 5 Paste Select the user sub item under equipment option in the equipment list or equipment option in the equipment list and click paste or right click paste in the menu to paste the user and the program file under the user name If the copied user has already existed it will prompt whether to cover when pasting If the program file under the existing user name is in use then pasting cannot be carried out Pasting may be finished by dragging mouse j 6 Import user Browse For Folder Click import to select the folder saved by the user in the pop up dialog box Click OK to import user Please select import user If the user selected has no program file then user cannot be RE Desktop b ag Libraries me lite If there is same user name then it will prompt whether to cover in 4 Computer importing gt amp OS C TP b cg DATAPARTI D If the program file under the same user name is i
36. on automatic file connection gradient temperature setting the maximum paragraph step and cycle times for editable file are 10 100 and 99 respectively The scope for gradient temperature setting is 1 C 30 C b Save the file c Revise the file adjust the rate for temperature rise fall refine temperature and time for cycling automatically and display and calibrate the time d Display the temperature time cycle times setting and real time value during the file running including sound prompt total rest time estimation for file running failure protection and alarming e Stop the file running including pause the file running stop the file running recover automatically after power off Caution The above mentioned software function is merely for reference The software function would be modified without notice Instruction Manual of LifeTouch Thermal Cycler CHAPTER Ill PREPARATION This chapter mainly introduces structure of the instrument and preparation before startup When using the instrument for the first time read the contents in this chapter carefully before startup 3 1 Structure diagram Hot lid Touch screen If USB interface Pen buckle Fixed location Touch pen Installation method Caution The fixed location can be adjusted according to your need Take the touch pen out of the accessory and strip the When fixing the location must be determined Then strip the pen 3M r
37. system main menu Setting interface of PCR program 4 1 1 Add or delete program segment You can add or delete program segments by clicking segment or delete J at the bottom of the screen on the setting interface of PCR program 98 0 98 0 98 0 98 0 98 0 00 30 00 05 00 30 00 05 7210 12 0 00 20 01 00 00 05 2 lt P Y r2 3 n p P ECH REECH 9 zc Back Save Run Seg Goto Delete Back Save Run 4 1 2 Program and constant temperature time You can set temperature of program segment and constant temperature time by clicking temperature or time on the screen on the setting interface of PCR program 00 30 00 05 720 72 0 00 05 72 0 72 0 62 0 00 20 01 00 00 05 ve de 00 05 Temp Setting Add program segment Instruction Manual of LifeTouch Thermal Cycler 4 1 3 Cycle function On the setting interface of PCR program first click the program segment that needs to add cycle function Then click jump CS to enter the setting interface of cycle function as shown in the right figure Junp Setting Repeat Times gt 0 Seg 03 98 000 05 5 6 Save Run The jump function takes effect only when it jumps from the later program segment to the former one See as the above figure seg05 seg03 In setting you need to enter the program setting status of seg05 then click jump to set cycle function 98 0 98 0 Eos 00 30 00 30 00 05
38. t COMS Select all Unselectall v Automatic connection 4 Add instrument Click add instrument a and a dialog box like the right figure will appear Input instrument name and Add instrument address Click OK to add instrument The instrument name shall not be empty and the max Instrument name bytes shall be 12 Only numbers and letters are allowed in the instrument gu a aes name Instrument address D 0 5 5 1 The same instrument name is not allowed The instrument address shall not be empty and the same address is not allowed to be used in different instruments 5 Edit instrument Select equipment name sub item in the equipment list and click edit instrument amp amp An equipment edition dialog box like the right figure will pop up input instrument name and instrument address When connecting the instrument the instrument address cannot be edited 6 Remove instrument Select equipment name sub item in the equipment list and click remove instrument amp amp The instrument cannot be removed when it is connected 7 Show all instruments Click all instruments and a list of all instruments will appear to show all instruments information 8 Instrument system information Select system setting a setting dialog box like the right figure will appear Instrument system information contains beep alarm key sound completion sound temperature keep sound and hot l
39. t Monitor Qi IP Address Report Message Log 0 Monitor Log 0 No Time Description 0 0 0 Now 2008 1 3 14 15 25 X Network Enabler Administrator Configuration E BK File Function Configuration View Help a2 eon Function Configuration 2 Network Enabler Module s EM Network Enabler Admi No Model MaCAddress IPAddress Configuration 1 NE 41105 00 30 8 14 60 78 192 168 0 154 i d COM Mapping 2 NE 41105 00 30 8 18 0210 192 168 0 103 Monitor Port Monitor XX IP Address Report 2011 4 13 14 32 14 Found Network Enabler Module s 2 2011 4 13 14 32 43 COM Port Deleted 192 168 0 154 Port COM2 2011 4 13 14 32 43 COM Port Deleted 192 168 0 103 Port1 COM 4 2011 4 13 14 32 43 COM Port Added 192 168 0 154 Pot COM 2011 4 13 14 32 43 COM Port Added 192 168 0 103 Port COMS Now 2011 4 13 14 45 38 Configuration Information Auto Warming IPAddress Report Password DigitallO Serial CMD Model e gt Network NE 41105 Basic Serial perating Mode Accessible IPs lv Modify IP Address 192 168 0 245 MAC Address 00 90 8 10 E2 B7 Serial Number 3898 Netmask 255 255 255 0 Gateway Firmware Ver Ver 4 0 IP Configuration Static DNS Server 1 BIOS Ver Ver 21 DNS Server 2 Status Modify Data Mode Enable SNMP Community Name public Location Contact PEPE
40. tinuing Click Next to continue or Cancel to exit Setup ie Setup ThermalCycler Select Destination Location Where should ThermalCycler be installed LJ Setup will install ThermalCycler into the Following Folder To continue click Next IF you would like to select a different Folder click Browse E Bioer Thermalc cler At least 2 7 MB of free disk space is required DL Setup ThermalCycler Select Start Menu Folder vi Where should Setup place the program s shortcuts S Ss Setup will create the program s shortcuts in the Following Start Menu Folder To continue click Next IF you would like to select a different Folder click Browse menn Con 18 19 Instruction Manual of LifeTouch Thermal Cycler 4 Click install 5 Finish installation Click finish 5 2 2 Install tool software 1 Click and run nead setup exe 2 Click Next Ready to Install 5 Setup ThermalCycler Setup is now ready to begin installing ThermalCycler on your computer Click Install to continue with the installation or click Back if you want to review or change any settings Destination location C Bioer Thermalcycler Start Menu Folder ThermalCycler y Setup ThermalCycler Completing the ThermalCycler Setup Wizard Setup has finished installing ThermalCycler on your computer The application may be launched by se
41. tremely careful operation of a certain step Failure to observe the instruction would result in serious personal injury 2 Safety During operation maintenance and repairment of this instrument the following basic safety notes shall be observed In case of failure to follow these measures or the warnings or notes indicated herein the basic protection provided by the instrument its safety criteria of design and manufacture and its predicted use range would be impaired Hangzhou Bioer Technology Co Ltd shall be irresponsible for the consequence resulted from user s failure to observe the following requirements Caution This instrument conforms to Class I and Protection Class IP20 of GB4793 1 Standard and it is for indoor use This product has been tested to the requirements of CAN CSA C22 2 No 61010 1 second edition including Amendment 1 or a later version of the same standard incorporating the same level of testing requirements a Instrument earth In order to avoid electrical shock accident the input power wire of instrument shall be reliably earthed This instrument uses 3 core earth plug and this plug is provided with a third earth leg is merely used for earth type power socket and is a safety unit If the plug is impossible to insert into the socket the socket shall be correctly fixed by electrician otherwise the earth plug would lose safe protection function b Keeping apart from the live circuit The operator shall
42. truction Manual of LifeTouch Thermal Cycler 5 4 Run software 000 Comprise user management file management L d d and equipment management etc Export Create Ed n Import Menu bar Instrument contents File display and edition area Display area of running status Status bar a e c 5 4 1 User management Include creating renaming deleting copying pasting importing and exporting user etc E d d a RI o T Rename Delete Copy Export User operation 1 Create user Select equipment name sub item in equipment list Create user and click create f Or right click create user The user name shall not be empty The max length of user name is 12 bytes The user name shall only contain numbers and letters under half width mode OK A same user name cannot be used for more than one time in a same instrument Do you want to create new user in Computer Enter new user name The user name can contain only letters and numbers 2 Rename Select the user needing to be renamed in the equipment list and click rename or right click alter user name Click OK to save after inputting user name It cannot be renamed when the file under the user name is opened Change user name Do you want to change user name of Computer s test Enter new username The user name can contain only letters and
43. ubber release layer at the back of We pen buckle buckle off from the back 3M rubber release layer and fix it on the after determining the fix location Then paste it on the instrument fixed location as shown Module Ventilation hole RJ45 interface Power switch Power socket with fuse Caution The fuse specification must be 250V 8A 5 20 Use qualified product to replace the fuse Contact fuse supplier or manufacturer when necessary 3 2 Examination before startup Please confirm the following contents before plugging in for powering on 1 Whether the power supply conforms to the required voltage see power supply requirements in Chapter II of the file 2 Ensure the plug of power cord has been reliably plugged in the socket 3 The power cord is reliably grounded 3 3 Startup Turn on the power switch turn the switch at The Thermal Cycler will produce one toot sound It indicates that it is powered on At that time the screen is still black Please wait for about 10 20 seconds The main menu will then appear Now you can edit browse alter and delete PCR Thermal Cycler file as well as other operations Instruction Manual of LifeTouch Thermal Cycler CHAPTER IV OPERATION GUIDELINE This chapter provides detailed introductions of the methods for the edition browsing alteration and deletion of the PCR THERMAL CYCLER file its running as well as setting of instrument system parameters etc Caution
44. well as its basic parameters performance and functions 2 1 Normal working conditions Ambient temperature 10 C 30 C Relative humidity lt 70 Power supply 100 240V 50 60HZ Caution Before using the instrument please make sure the working condition meets the above requirements Especially the power line must be reliably earthed 2 2 Transportation and storage conditions Ambient temperature 20 C 55 C Relative humidity lt 80 2 3 Basic parameters Product name Product No Model Module model Sample size LCD display Temperature range Max heating rate Max cooling rate Temperature uniformity Temperature control precision Temperature fluctuation Gradient temperature range Gradient temperature range Temperature range of hot lid File saving amount Energy consumption Operation mode Dimensions Fuse PC interface Net weight Life Touch BYQ6072 TC 96 G H b B B 96GA 96 holes x0 2ml 96 holes full apron plates half apron plates no apron general and 12x8 test tube bar Color touch screen 6 5 260 000 colors 4 105 C gt AT Ze SAC is 020 s BU 165 0 202 CU over 90 C 02 CU 300 105 C LE 300 30 C 1100 gt 250 600W Continuous running mode 345 x 250 x 270mm 250V 8A 5x20 RJ45 10kg 2 4 Software functions a Edit the file including file number temperature and time of each segment cycle times setting file view revision and deleti
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