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Panasis User Manual

1. RGB merge E P120727 2 55 37 Sle E J 8 P120727 3 5562 Ej P1207274 55 75 Sl RGB 8bit image E P 1207276 56 00 merged image HSV merge E P120727_23 56 00 P1207278 56 00 ahi P1207279 55 87 Merged image 8 7 Manual multi focus Manual multi focus synthesizes a focus enhanced image an image of extended depth of field from images whose focus regions are different each other Manual EDF Image List Available Images Selected Frame mosquito 1 f00 jpq mosquito _1 f0L jpg mosquito _1 f02 jpq mosquito_1_f03 jpg mosquito_1_f04 jpg mosquito 1 f05 jpg mosquito 1 f06 jpg mosquito _1 f07 jpg mosquito _1 f08 jpq mosquito_1_f09 jpg Option Focus analysis options Max local contrast Pl Generate topographic map A Displacement correction 0 image s in stack Available Images shows all opened images If there is an image in the Selected Frame the Available Images list is filtered by the image size of the image Selected Frame shows all selected images for multi focusing Add All This button moves all images in Available Images into Selected Frame This button is always activated gt Add This button moves images chosen in the Available Images into the Selected Frame This button is activated when an image in Available Images is chosen You could move multiple images with Ctrl
2. Arrow This button creates an arrow pointing to the starting point Freehand This button creates a free line by mouse dragging Polyline This button creates a polyline If mouse is double clicked the creation ends Rectangle This button creates a rectangle Polygon This button creates a polygon If mouse is double clicked the creation ends Ellipse This button creates an ellipse using two points Point This button creates points Text This button creates a text box where user could write Annotation Properties This button creates a window where user sets the annotation properties such as colors transparency Note To save a flattened image set the Save as a flattened image in the Environmental Option on or flatten the image using Flatten in the Image menu If you do not use these command annotation objects are not saved The saved objects could be edited Note Since the Annotation tools window is DockingPane the window could be attached anywhere When the window is overlapped to other DockingPane each DockingPane is distinguished by Tab 10 1 1 Annotation properties If the Annotation properties button is clicked the following window appears If no object is selected the property is applied to all objects created after the property changes If any objects are selected then the property is applied to the ones Annotation Properties Line Properties Font Properties wath pt
3. In 3D view zoom in and zoom out interface are same as in 2D mouse wheel controling Drag mouse on the image to rotate the image 5 10 3 3 Color When la button is clicked Panasis uses an EDF image for the surface mapping 5 10 3 4 Mesh When HH button is clicked Panasis uses a wireframe image for the surface mapping The image on the wireframe is a pseudo color image which reflects the height information The wire intervals are set to one pixel now 5 10 3 5 Pseudo Control the slide bar of Pseudo to decide the blending ratio of a pseudo color image and a color image for the surface mapping On the right hand side of the slide bar there shows the percentage of a pseudo color image in the texture image 5 10 3 6 Z scale Control the slide bar of Z scale to decide the zoom ratio of Z axis with the other axises fixed On the right hand side of the slide bar there shows the current zoom ratio of Z axis 5 10 3 7 Lighting Panasis gives lighting effect to generate shadows of the 3D image The shadows are useful to surface analysis of the sample Push Light button to turn on or turn off the lighting effect lt on gt Lighting off gt Picth This bar controls the latitude of the lighting Yaw This bar controls the longitude of the lighting 5 10 4 Toolbar 3D Profiler Select and run a 3D File function Protile Measurement Select and run a measurement theme t 2357143 h3d
4. Preview Preview shows a live image of the camera Snap Snap captures an image from preview window or Live measurement window and 15 6 Calibration Toolbar EL ie Calibration Scale Bar Live Mist This box selects the calibration Micrometer um 7 Unit combo box This box selects the calibration unit a Calibration This button opens the calibration wndow where user could create new calibrations 100 Scale bar This button shows scale bar i for ut Live Measurement This button shows the live measurement window 15 7 View Toolbar Navigator Gallery Navigator this button shows the navigator window Gallery this button shows the image gallery The End
5. Advanced this button turn on the advanced mode where all camera controls are accessible 6 1 1 1 How to execute white balance for Panasis Supporting cameras How to execute white balance for Panasis supporting cameras 1 When Camera Setting Open is clicked in the Camera Properties window the following camera controllers are shown Camera Properties Device LUSIS HC 50MU 24214371 romat Reago E es so E White Balance One Push Exposure Exposure 255 Shutter Gain Flip A Horizontal Flip Vertical Flip 2 put it on the focal position of camera a piece of white paper or a sample for the white balance set up 3 Adjust shutter control or turn on auto shutter mode to make a camera image have the proper brightness 4 Push the One Push button Note It is recommended to set the camera shutter mode on Auto mode when focus ratio or lighting condition changes 6 1 1 2 How to execute white balance for DirectShow cameras How to execute white balance for DirectShow cameras 1 When Camera Setting Open is clicked in the Camera Properties window the following camera controllers are shown Camera Properties 25921944 32bit 0 7 fps RGB32 White Balance U B A Horizontal Flip C Vertical Flip 2 Push the Advanced button The components of advanced camera set up could differ for each Camera Video captur
6. All image files are listed as a file name list If a file name is chosen then the file is activated Acquire Menu Camera Properties Preview F5 Snap Space HDR Capture Time Lapse Record Video Overlay Focus Indicator Live Multi focus Live Tiling Select TWAIN device Import TWAIN image 6 1 Camera Properties You could choose the camera from the registered camera list and the resolution of the camera For even one camera two items could be listed Cameras with names including WDM are controlled by DirectShow drivers If the camera used last is still connected then the camera is automatically selected when Panasis Starts How to select a camera 1 If Camera Properties from Acquire is run the following device property window shows up device This box selects the camera from the list of cameras which are accessible now Format This box selects the resolution from the resolution list of the selected device FPS This button selects frames per second for the corresponding format from the FPS list Preview This button opens and closes the preview window Camera setting Open Close This button opens and closes the camera setting window Camera Properties Device LUSIS HC 20CU la mat 600x200 Camera Setting Open 2 The available cameras are listed in the device box Please select the camera you want to use Camera Properties MEA Device LUSIS HC 20CU Format WDM DMC CCD WDM
7. Line Color Line Transparency Fill Color E Fill Transparency Start Style iz End Style Style This box determines the style of the line solid line dashed line etc Thickness This box determines the thickness of the line 1 5pt Line Color This box determines the color of the line Line Transparency This box determines the transparency of the line 0 25 50 100 Fill Color This box determines the color to fill the inside of the figures Fill Transparency This box determines the transparency of the figures insides Start Style This box determines the style of a starting point of a line arrow big arrow bar etc End Style This box determines the style of a end point of a line arrow big arrow bar etc Annotation Properties Line Properties Font Properties SS Transparency Bold l Obligue Underline K Caneel Font This box determines the font of texts Size This box determines the size of texts Color This box determines the color of texts Transparency This box determines the transparency of text boxes Effect This box gives the effects on texts Bold Oblique Underline Note The activated properties depends on annotation tools 11 Microscope Option Device Control Z Axis Motor Control 11 1 Device control Option Device control controls the Huvitz Upright Microscope This function is provided in Panasis UPRIGHT
8. DMC CMOS WDM DMC Legacy WDM TCA 3 0 Camera WDM FireWire digital camera WDM Crevis a T f FPS 3 The available resolutions are listed in the resolution box The changed resolution is immediately applied to Preview window and Live measurement window Camera Properties Device LUSIS HE 40CU Format APS 4 The available FPS Frames Per Second are listed in the FPS box The changed FPS is immediately applied to Preview window and Live measurement window Camera Properties A Device LUSIS HC 50MU 24214371 Format RGB24 1024x768 Note If the connectted camera is not shown in the list please check whether the camera driver is installed properly FPS function could not work for some cameras 6 1 1 Camera Setting The camera configurations of white balance exposure and so on are set When the Advanced button is clicked Advanced mode is turned on and all configurations supported by the camera are shown In the case of DirectShow camera please set the camera configurations in the Advanced mode because all items in the camera setting are in deactivated states Camera Properties Device LUSIS HE 50MU 24214371 One Push Fl Horizontal Flip C Vertical Flip save Open this button opens a saved camera set up Save this button saves the current camera set up Default this button returns the current setup to the default camera set up
9. Name Date modified w Favorites ME Desktop coin h3d 10 25 2012 6 47 AM H3D File Recent Places Je Downloads E MyDocuments Libraries a Music t Pictures E Videos v g mw p File name Panasis z x Save as type Panasis 3D Image h3d 5 10 1 3 Close File Close a image file which is displayed now 5 10 1 4 Capture 2D Image Capture 2D Image saves the 2D view of the activatd image Profile tools are flattend to the saved image 2D captured image of a sample applied by Volume Surface by Profile 5 10 1 5 Capture 3D Image Capture 3D Image saves the 3D view of the activated image as a 2D image file 510 200 481 896 453 593 552 300 u 414 225 276 158 138 675 294 560 441 840 589 120 X 736 400 um 3D captured image of a sample applied by Volume Surface by Profile 5 10 1 6 Capture Profile Image Capture Profile Image saves the image on the profile window 5 10 1 7 Save Measured Datalcsv Save Measured Datal csv saves the measured data and statistics of the activated Measure Theme in the activated image as a csv file 5 10 1 8 Save Collected Data csv Save Measured Datalcsv saves the data and statistics in the Collected Data window as a csv file 5 10 1 9 Load from 2D Files Load from 2D Files opens a 3D image by opening 2D height map image and 2D texture image A Open 2 Files for Height Map and Texture Image Open
10. Data window e The reference point does not have the height data e The reference line is green The selected measurement line is red e When a reference line is chosen the other measurement lines using the referenece line are green 5 10 2 3 Volume Surface Measurement This measures volume and surface area of a sample The followings are detailed themes 1 Volume Surface by Profile 2 Volume Surface by Histogram 5 10 2 3 1 Volume Surface by Profile Volume Surface by Profile measures the volume and surface area of the specified region with a threshold applied on the profile line 1 Select Volume Surface by Profile from measurement theme 2 Select a profile tool and specify the profile line Place a horizontal line on a specified Horizontal line o position to get a profile line Place a vertical line on a specified Vertical line o o position to get a profile line Click two points to generate a line for Line oe a profile line e Only one profile line is allowed in Volume Surface by Profile 3 Choose a threshold option Only the region whose AS Beyond the threshold profile value is beyond the threshold is measured Only the region whose Below the threshold profile value is below the threshold is measured 4 Choose a measurement option The inner region of the profile line Peak satisfying threhold condition is measured The outter region of the profile line Valley satisfy
11. Distance between 2 circles This button measures the distance between two circles which are created by 6 clicks to define two 3 point circles This function belongs to macro measurement The result data are same as that of line CR 1 78 62 pixel Distance between line and points This button measures the distance between a line and points The number of points are not fixed This function belongs to macro measurement The result data are same as that of line PT 7 53 95 373 01 DT 6 129 85 pixel 7 k Py 7 i kn gt Y 4 o DT 8 144 04 pixel 00 PT 5 327 10 469 64 LN 4 252 72 pixel Angle between 2 lines This button measures the angle between none intersecting two lines The result data are same as that of line Auto Trace This command measures the length of auto detected polyline The number of points to create an Auto Trace is not fixed When the mouse is double clicked at the point Panasis starts automatically trace a circumference to the right hand side of the point or the direction connecting two points the double clicked point and the starting point Panasis recognizes points having the largest contrast edge points The result data are same as that of polyline Note To save an image and measurement objects without flattening file format should be tif format To save a flattened image set the Save as a flattened image in the Environmental Opt
12. ECO is a function which changes the miscroscope mode into the ECO mode where all lighting powers are off when a user has disappeard in front of the microscope for the ECO waiting time When a user is back in front of the microscope ECO mode is off 11 2 Z Axis Motor Control Option Z Axis Motor Control controls a Huvitz 3D Profiler This function is provided in Panasis 3D Profiler Before starting Z Axis Motor control connect Panasis to the microscope and register objective lenses and calibarion data as explained in HUVITZ Upright Microscope The left controller is for COM port is intialized and the right one is for COM port is not initialized Z Axis Motor Control x Z Axis Motor Control Current position Current position a am Pros lt COM port is initialized gt lt COM port is not initialized gt 11 2 1 Z Controller 1 z Navigator The blue circle in the center means the current position of the microscope head the head The position tags indicate the positions of origin for 0 Bottom Limit for BL Top for T and Bottom for B When the position tag is clicked the head moves to the position 2 Movement button button moves the head up y button moves the head down The bigger arrows the large movements 3 current position This shows the curruent position of the head This could change the value when O position is newly set or absolute postion box is chec
13. IFAD MOM peer tren ens A nS Tw OTT fe CRIES Neem RTT ea 201 14 1 ADQUE osuna cido estas eb eos poi Or TEER TPES 201 15 Toolbar 202 15 1 FUG A E TA 202 15 2 A 202 15 3 Mouse Mode Toolbar sesa ds ene eS aye 203 A eee nena rea Teer ener ede eee rene ern eee 204 155 AACQUIFe TOO DF iiss tawacavadawadcavadawndadadawadadadawadetadadadatadadadavadasad 204 15 6 Calibration TO CDA o eeree 205 15 7 MIEW TOO DIE 2 a a een AEN E ESE En IIS a A TA 206 1 Help Panasis Copyright 2011 Huvitz All rights reserved This help manual explains the overall functions of Panasis Each items are arranged according to Graphic User Interface GUI the order of menu or tool bar Panasis could be distributed as different versions and different Plug In modules Therefore the help manual could explain the functions your software does not have Vice versa the module you purchased could not be explained in this manual In that case please refer to the manual served with the module 2 System Specification The system specifications for running Panasis is the following IBM PC Compatible Minimum Pentium 4 2G Hz 512MB or more RAM Pentium 4 Dual core Recommended 1GB or more RAM NVIDIA GeForce8400 Microsoft Windows 2000 XP Vista 7 3 File Menu NW New Image Ctrl N E Open Ctrl 0 E Close Ctrl F4 Mm Save Ctrl S Mm Save as Ml Crop M Environmental Option Mm Exit Alt F4 3 1 New Imag
14. Profile from Measurement Theme 2 Choose an option among the Compare option The height difference between 2 Points two specified points is measured A reference point is specified first The height differences Reference between the point and newly added points are measured 3 Push buttons to specify measurement position e A measurement ends when right button is clicked e The result data is listed on the Data window e The reference point does not have the height data e The reference line is green The selected measurement line is red e When a reference line is chosen the other measurement lines using the referenece line are green 5 10 2 2 3 Height by Histogram Height by Histogram creates a height histogram on specified region and measures height differences on the histogram 1 Select Height by Histogram from Measurement Theme 2 Select the method to specify a region to create a histogram 3 Specify a region to create a histogram Only whole image is possible for the region specifying now 4 Choose an option among the Compare option The height difference between 2 Points two specified points is measured A reference point is specified first The height differences Reference between the point and newly added points are measured 5 Push buttons to specify measurement position e A measurement ends when right button is clicked e The result data is listed on the
15. Top position to set is below the Bottom position set before this position could not be set as Top Set the position as Top after setting the lower position as Bottom If Bottom position to set is beyond the Top position set before this position could not be set as Bottom Set the position as Bottom after setting the higher position as Top 11 2 3 AF AF is short for Auto Focus which automatically adjust the focus of the specified region When button is clicked the AF menu appears under the z controller and live window shows up with a rectangle 1 Specify the region to apply AF by using the rectangle Adjust the size and the position of the rectangle on the Live window to specifiy AF region CM button in the ROI changes the color of the rectangle 2 Choose the scanning process Current pos When ca button is clicked the head moves from the current position to BL for AF Current pos Range When button is clicked the head moves around the current position with the range set by a user Also the AF control menu changes as the following Z Axis Motor Control N Current position u lab Current pos BL 15um 30um 60um Speed Auto select A RON as y A user could manually input the scanning range in the Range box Also 3 Set the scanning speed 15um 30um buttons could be used to set the scanning range Panasis has a five level
16. a 2D image file for a height map Open Height Map di gt panaiss Y My Documents Y Panasis Images Y Surface View Organize v New folder v Hl Sk Favorites Name i Date modified Type Size W Desktop _ gear p3d 10 25 2012 6 47 AM P3D File 13 1 Recent Places __ LaregWheel p3d 10 25 2012 6 47 AM P3D File 3 Je Downloads gia LargeWheelTexture 10 25 2012 6 47 AM BMP File 1 E MyDocuments gia LargeWheelTopo 10 25 2012 6 47 AM BMP File 1 __ small_wheel p3d 10 25 2012 6 47 AM P3D File 13 Libraries a Music Pictures E Videos a MyDocuments Ba v 4 m 7 File name LargeWheelTopo z All Files Open a 2D image file for a texture image Open Texure Image de v panaiss Y My Documents Y Panasis Y Images Y Surface View Organize v New folder A Fe P Xx cita Name Date modified Type ME Desktop gear p3d 10 25 2012 6 47 AM P3D File E Recent Places _ LaregWheel p3d 10 25 2012 6 47 AM P3D File WB Downloads aii LargeWheelTexture 10 25 2012 6 47 AM BMP File E MyDocuments gia LargeWheelTopo 10 25 2012 6 47 AM BMP File _ small_wheel p3d 10 25 2012 6 47 AM P3D File Libraries a Music t Pictures E Videos 1 MyDocuments v d File name z All Files Note Even though the size of a heigt map image and that of a texture image are different a 3D image could be generated 5 10 1 10 Reset Layout Reset Layout makes the screen layout back to the initial sta
17. and shows the data and the statistics of that How to use Data Collector Select data in the Measurement data table and push Collect button Collected Data Measured Data Collect Delete Delete As2 2 v 6 2 7 Valley Pido 7 25 2 7 26 2 v 1 27 Valley Width 257 79 um 257 79 um 130 44 um 27 13 um This button deletes the selected data Height 49 06 um 49 06 um 33 76 um 0 67 um Length 262 42 um 262 42 um 134 73 um 27 14 um Surface le 286 23 um 286 23 um 155 61 um 27 3 um Angle 0 19 radian 0 19 radian 0 25 radian 0 02 radian Statistics This table shows statistics of the collected data Measured Data Collect Delete Mean Std Deviat Sum Num of Sa Note Data collector could get together all data regardless Width 27 13 um 251 19 um 168 44 um 96 45 um 673 75 um 4 Height 0 67 um 49 06 um 33 14 um 19 76 um 132 55 um 4 Length 27 4 um 262 42 um 171 83 um 98 17 um 687 31 um 4 of measurement themes Surface le 2773 um 286 23 um 188 95 um 107 27 um 755 79 um 4 Angle 0 02 radian 0 25 radian 0 16 radian 0 08 radian 0 65 radian 4 5 10 3 Image Option Panasis provides various image options to enhance the observation 5 10 3 1 2D View 2D When button is clicked image window converts into 2D view 5 10 3 2 3D View 3D When button is clicked image window converts into 3D view
18. beyond threshold option is chosen the threshold plane is bright colored When below threshold option is chosen the threshold plane becomes transparent 5 10 2 4 Measurement Data The measured data are listed on the data table Measurement Each sample image has own measurement tables which consist of different tables according to the measurement themes If an item is selected in the table the corresponding measuring object is selected too Vice versa if a measuring object is selected in an image the corresponding data are selected too Push delete key to delete the activated objects Measured Data Collect Delete Volume Surface area Area Surface area rea 23 Rectangle 908165 25 urm 45769 77 umn 41056 32 umn 1 11 Statistics Collect es This button collects seleceted data to data collector Delete pla This button deletes all measured data Statistics This table shows statistics of the measurement data selected now Measured Data Volume Surface area Area Surface area Area 908165 25 um 45769 77 um 41056 32 um 1 11 908165 25 um 45769 77 um 41056 32 um 1 11 Mean 908165 25 um 45769 77 um 41056 32 um 1 11 Std Deviation 0 00 um 0 00 um 0 00 um 0 00 Sum 908165 25 um 45769 77 um 41056 32 um 1 11 Mum of Samples 1 1 1 1 Note When measurement theme changes measurement data table changes too 5 10 2 5 Data Collector This function collects the measured data
19. button measures the maximum minimum distance between two objects These buttons are activated when the following objects are selected point and rectangle point and polygon line and line point and circle point and arc circle and circle line and ellipse point and line line and circle line and rectangle line and polygon DT 3 327 39 pixel em DT 4 60 71 pixel gt oe LN 2 197 33 pixel Perpendicular distance This button measures the perpendicular distance between two objects This button is activated when the following objects are selected line and ellipse point and line line and circle line and rectangle line and polygon line and arc LN 2 197 33 pixel Combination line There are three options line segment tangent line division line This button is activated when at least one of above options is available It has data the same as line has line segment This button is activated when two points are selected It measures the distance between two points LN 3 185 62 pixel Tangent line This button is activated when two circles or a point and a circle are selected It measures the length of tangent lines between two objects LN 67 a LN 9 143 00 pixel LN 8 143 00 pixel Division line This button is activated when an angle is selected The number of the divided parts is the same as the number below the button Kd i 7 n Di 12 172 8
20. file 13 Window Menu MW Cascade MW Tile Horizontally M Tile Vertically M Close All 13 1 Cascade F9 Cascade command arranges opened image windows so that they are roughly equal sized and laid leaving only their title bar and left border visible Minimized windows remains still minimized FineGrane png 100 00 13 2 Tile Horizontally F10 Tile Horizontally arranges the opened image windows horizontally Minimized windows remains still minimized E mosquito_1_105 jpg 200 00 AchromaticTest bmp 70 00 large dia wheel o type 82um 30 1X 02 jpg 100 00 13 3 Tile Vertically F11 Tile Vertically arranges the opened image windows vertically Minimized windows remains still minimized mosquito_1_f05 jpg 200 00 l N lola 3 A 13 4 Close All Shift F4 This function closes all image windows If an image window is not saved when the image changes the following dialog shows up and ask whether user would save it or not Close All C120727_7 Would you save the image Yest0 d This button saves the current image and closes it Yes Aug This button saves all unsaved image and closes them Now This button does not save the image and closes it No ANO This button does not save all unsaved image and closes them Cancel c This button cancels window closing 14 About
21. horizontal scale bar Vertical If this box is checked Panasis shows a vertical scale bar Text size This box sets the text size of the scale bar Color This box sets the color of the scale bar Thickness This box sets the thickness of the scale bar Division This box sets the number of divided parts in the scale bar Position This box decides the position and the shape when Horizontal and Vertical boxes both are checked of the scale bar Default calibration pixel Horizontal 100pixel Calibration applied Horizontal and Vertical all checked Upper Left 0nm 10 Annotation menu E Annotation tools 10 1 Annotation tools Mouse cursor is different for each Annotation item Annotation objects are created by left click and they could be created in succession after the end of an object creation In the case of that it is fixed the number of points which consist of an object right click cancels the creation of the object and Panasis gets back to selection mode where the shape of the mouse cursor is an arrow Otherwise e g point right click complete the object creation and Panasis continues object creation mode All procedures of creating deleting and editing for measurement objects supports Undo Redo function Also You can copy and paste them Panasis provides the following annotation tools Annotation icy Sam a ses ti Line This button creates a line connecting two points
22. of scanning speed However Panasis recommends auto mode where the speed is automatically set according to the scanning range 4 Push button to start AF Push Cancel button to stop the process 11 2 4 AH AH is short for Auto Height which measures the height difference between two specified regions When AH button is clicked the AH menu appears under the z controller and live window shows up with two rectangles speed Quo sec Y cos A o If the proper scanning range is already set skip 1 4 1 Move the microscope head head to the top position of the sample using z controller 2 Push zt button of Top to set the current position as the upper limit of the head movement This position is shown as T in the Navigator 3 Move the microscope head head to the lowest position of the sample using z controller Set y 4 Push button of Bottom to set the current position as the low limit of the head movement This position is shown as B in the Navigator 5 Specifiy AH regions by adjust the size and the position of the rectangle on the Live window If ra or 2 button is clicked the corresponding rectangle is editted E O Y button in the ROI changes the color of the rectangle 5 Set the scanning speed Panasis has a five level of scanning speed However Panasis recommends auto mode where the speed is automatically set according to the scanning range 6 Push Run
23. of that each region has own brightness It could take several minutes according to the size of an image or the system specification HDR Ca pture It may take several minutes Would you like to run The result of HDR Capture snap HDR result Note HDR Capture is not available for some cameras 6 5 Time Lapse Time Lapse snaps images from a camera with the assigned time interval This function Is available only when Preview window Is activated Time lapse Frame Total Current Interval Total type the total number of images to snap Start Push Start button to starts to snap Stop Push Stop button to stop snapping 6 6 Record video Record video records video from Preview window It is available when Preview window is activated Panasis uses Video Codec supported by user s PC so the maximum recording time depends on the Codec But the maximum size of the video file is limited up to 2 GB Record video Start This button starts a video recording When a video recording starts Start button changes into Restart button Pause This button pauses a video recording Restart This button restarts a video recording Stop This button Stops a video recording Options This button shows an option window which contains a time interval controller an image encoding selector and the play speed controller Record video Options Capture Interval o H o M o S 8
24. on the FP out focused upper the FP Note Focus Indicator is for the cases to investigate the focus changes for a specific region It is not for comparing focus values of different regions of an image 6 9 Live multi focus Live multi focus synthesizes a focus enhanced image an image of extended depth of field from live images whose focus regions are different from each other It is available only when Preview window Is activated Start Live multi focus Live Multi tocus E Create Height Map Position Correction During Live multi focus When Live multi focus starts the green spot is shown on the upper right corner of Preview window Green spot means multi focus works well When the spot changes red you should move back the sample to the last position where green spot was because the red spot means Live multi focus has a problem If the spot is back to green Live multi focus works well Preview 28 38 Live multi focus stop If the Stop button is pushed Panasis synthesized a multi focus image from images collected by that time Panasis might create a height map if Create Height Map box is checked If the Cancel button is pushed Live multi focus process is canceled Live Multi focus Create Height Map The result image of Live multi focus At the end of Live multi focus process the following two images are created in new windows Multi focus image He
25. selected Max Intensity This method is used for samples that are more intense than the background such as light emitting flourescent dyes The pixel from the plane having the highest intensity at the current pixel location is selected Min Intensity This method is used for regions that are darker than the surrounding area to be in focus The pixel from the plane having the lowest intensity at the current pixel location is selected Generates topographic map Clicking this button creates a topographic map from the focused image Displacement correction When this button checked Panasis calculates the displacements between input images and compensates them The result of Manual multi focus input images the result image and the topographic map 8 8 Manual Tiling Manual Tiling manually generates a tiled image from images of Preview window or saved files The overlapped region of images is seamless by the Panasis image processing Also Panasis provides the position compensation function which finds and compensates the displacement between images when moving or attaching an image Open This button opens images from files The opened images are listed in the gallery Capture This button captures images from a camera The captured images are not listed in the gallery They are added into the workspace Attach This button attaches a newly added image into the result image Fix posit
26. the RGB values of an image Best Fit this command makes the bright region of the image brighter and the dark region of the image darker Reset this command resets Brightness Contrast command status this command zooms in the preview image in the window this command zooms out the preview image in the window Apply this command applies the chosen commands to an image region 8 3 Hue Saturation The following image processes are provided If ROI is set then the image processing is applied to ROL All image process in Panasis support Undo Redo Hue this command changes the average Hue value of the image from 180 to 180 Saturation this command changes the average Saturation value of the image from 100 to 100 Lightness this command changes the average Lightness value of the image from 100 to 100 Reset this command resets Brightness Contrast command status this command zooms in the preview image in the window this command zooms out the preview image in the window Apply this command applies the chosen commands to an image region 8 4 Histogram Equalization This command performs the linear histogram equalization to make the image uniformly distribute the pixel values over the dynamic range 8 5 Split This command splits a 3Channel image 24 bit into three one channel images 8bit Panasis provides RGB HSV channel splits After Split three new windows with 8 bit images are ge
27. the X Y ratio box 4 Newly created calibration datum is now listed in the calibration set Note If the show guide line box is checked the mouse guide line is shown After Clicking the magnifier icon if mouse button is clicked the image is magnified 91 2 Add to calibration list This menu Measurement Calibration Add to calibration list is activated when the calibration datum of a loaded image does not exit in the calibration list This menu is clicked the calibration datum is added to calibration list 9 1 3 Apply calibration To apply calibration to images please use the calibration combo box in the tool bar If there is no calibration data in the list the box shows a default datum pixel To create calibration data please see the New Calibration page Default preel Default picel Ab um AS um ALO um X12 ur x15 um x20 um um 135 um x45 urn x5 7 2 urn If you select a calibration value in the calibration combo box the value is applied to an activated image The calibration combo box shows the calibration datum applied to the activated image If calibration list does not include the calibration data of loaded images the data are listed in the calibration combo box but they are gray letters which are not applicable to other images In this case Add to calibration list function adds them to the applicable calibration list Calibration units could be
28. 2 MS Real time capture Save Play Speed x When the Change button pushed in the option window the Encoding selection window shows up The Encoding list in the window is different for user s configurations Video Compression Compressor Microsoft Video 1 m Cancel Compression Quality 75 Configure 4 MT About Push the Start button Then set the name of a file and the directory to save the file After that the current recording status is shown in a progress bar If the file size goes to the maximum limit 2GB the recording automatically ends Note Panasis does not support a record player You should run another player to see a video 6 7 Overlay Overlay draws a cross hair grids including squares and concentric circles and a clock on Preview window or Live measurement window It is available when Preview window is activated Overlay A Cross hair Grid None 2 Inverted color Color E gt Position X Position Y Position Overlay burn in 5 None Current time Stopwatch Color rt I Font Style Arial Black a Position Upperleft O Upperright Lowerleft O Lowerright Overlay burn in None 2 Square Circle Color cor MEN Arial Black Overlay burn in Cross line It draws a cross line The crossing point is assigned by a user
29. 4 pixel Combination angle This button is activated when two lines are selected It measures the angle between the lines It has data the same as angle has Combination circle This button is activated when a point or a circle is selected The operation of the object is the same as that of 2 point circle It has data the same as circle has Parallel line This button is activated when a line is selected It creates lines parallel to the selected line by clicking their positions The creation stops when mouse right button is clicked It has the data the same as line has A LN 6 251 97 pixel A LN 4 251 97 pixel Note To save an image and measuring objects user should set the file format as tif To save an image flattened with measuring objects user should set Save as a flattened image on or save an image after applying Flatten in Image menu Note Since the combination measurement window is DockingPane the window could be attached anywhere When the window is overlapped to other DockingPane each DockingPane is distinguished by Tab 9 4 Live Measurement F6 It is possible to measure objects in the Live measurement window as in sill images The Live measurement window has the following features e It is shown as a black rectangle written by Live in the gallery e The image of Live measurement window is not shown in Navigator e Live Measurement window provides zoom in out overlay foc
30. 77 um 41056 32 um 1 11 441 846 ac Y Statistics gt 589 120 Profile Graph 510 20 487 56 lt Volume Surface by Profile 3D View gt Note In 2D view the region satisfying threshold option and measurement both is diplayed as a texture image and the other region is displayed as a pseudo colored image In 3D view when beyond threshold option is chosen the threshold plane is bright colored When below threshold option is chosen the threshold plane becomes transparent 5 10 2 3 2 Volume Surface by Histogram Volume Surface by Histogram measures volume and surface area of the specified region with a threshold applied on the histogram 1 Select Volume Surface by Histogram from measurement theme 2 Specify the region with region tool to measure volume and surface area e When a region tool buttion is clicked a region could be specified e The histogram is based on the data in the specified region e When a region is specified the result data is listed on Data window e Whenever a region button is clicked a region is created 3 Choose a threshold option Only the region whose height is Beyond threshold beyond the threshold is measured Only the region whose height is Below threshold below the threshold is measured Note In 2D view the region satisfying threshold option is diplayed as a texture image and the other region is displayed as a pseudo colored image In 3D view when
31. A elias 46 A O E a errr a 48 SIr II bo a 50 o AD CIPUE aa ner TEgTTT SER ERIE UOT Ie Wen Ew mer seu MnCRER Ure fee rnnnTT ERIE IER 51 aos OPINO ee 52 5 10 3D MeasurementiOption w ici caca iiios 54 ye 0 a n B npn oro Sno a nS OC ne ea Ce 55 3102 3D Measurement sn 63 DS Image ON ld de cold le 84 ILOA TOODA gr evet tenner menrnter rey teeta ate ere IEEE etree eee ere ees 86 6 Acg ire Mean idad 87 Sl Camera Oe perenne mr npr nm nro Er Pre Terre PTET Peer Peper Serre Pere 88 Cle ls AA II II II A All al ot 91 A PROV RCV A A A A 96 A to nro aE EE SaR One Soe TTT ESO eeEE Eye eee ee Seen een Tere eee 97 0 ADR Capture e o eee 97 a O Sa 99 OOs RECON A II 99 Tn o 101 HO FOCUS Indicato eee anette Rene nnn rene o Ds ee rere 106 Ive mul OCU S ot lb deb 108 6 10 PP ee 111 6 11 Select TWAIN device is cccccecccanadad adavscayacasadasadan acadadaradanacanadadadeinen 115 612 Aamport TWAIN IMA dd o 115 de MJE M N ld O Ta 117 PM RES E a caer ee et 117 Te RO eee Ae At Sete Ae ete eG aac 118 Los Grayscale e tac tetas ee etac ete eect 119 A o 120 Os POCOS MISA sin de tetas 121 ode A e o ld 121 0 BrROntness Contlas lisis ette tech dertr dba dns 124 8 3 HUe Satura lO sesccadisdai dd iii 126 84A Histogram EI A os 127 IS AA II A II A II E A erent aE Tem 127 a MA E 129 Se Manual MUITO CU Sid dl 132 88 MANUAL TINO A si 137 9 Measurement Me iia 141 AP o II A 141 9 1 1 New CAMO FALIO Dio cea 142 912 AA O calibr
32. End Y Pos Annie Y Cancel er This button adds the selected items in the left box to the right box Double click does same thing Add all This button adds all items in the left box to the right box lt Remove This button removes the selected items from the right box Double click does same thing Lx Remove all This button removes all items from the right box 9 7 Line Profile This command analyzes the color intensities of the specified line Line profile window is generated for each image If an image is closed the corresponding line profile window is closed too How to use line Profile 1 Open Live measurement window or still images 2 Run Measurement Line Profile 3 Locate and resize the profile line for the use How to move profile line Profile line means a line which appears in the activated image when user run Line Profile If mouse cursor hits the ends of the line mouse cursor changed into an arrow Then user could change the size of profile line by mouse dragging If you keep pushing shift key when dragging mouse the line becomes horizontal line vertical line or 45 degree inclined line according to the inclination of the line If the mouse hits the profile line then the mouse cursor changes into a four directional arrow Then user could move the profile line without resizing How to move Analysis guide line Analysis guide lines are cyan and pink line
33. Measurement is run Panasis view changes into the following a 3D Profiler Profile Measurement coin h3d EX g F A e ze n t y A nm 4 Pe d if Horizontal Vertical 2Points Aa E Length Area Angle Curvature Width Height Length Surface le Angle C S Area 498 88 487 56 476 24 464 91 453 59 5 10 1 3D File 3D File involves file open image save data save functions 5 10 1 1 Load File Load File opens a 3D image file of h3d extenstion amp Load File es Organize v New folder zz Hi A T i r Favorites Name Date modified Type HE Desktop coin h3d 10 25 2012 6 47 AM H3D File Recent Places Je Downloads E MyDocuments Libraries a Music t Pictures E Videos al MyDocuments 4 Ww File name leoink3d y Panasis 3D Image h3d y DD When more than five images are opened the warning message appears Panasis Insufficient memory may cause critical problems so please close all the unused files lt lt a Then please close files by Close File Note The image generated by EDF is automatically added to the opened file list 5 10 1 2 Save File Save File saves an image as a 3D image file of h3d extension The profile lines measuring tools on the image are saved together amp Save File di gt panaiss Y My Documents Y Panasis Y Images Y 3D Profiler Organize v New folder
34. Menu MW About 14 1 About This command shows the information of Panasis and the PC System Panasis Info Panasis Version 1 6 2 Copyright C 2011 2012 1623519016 System Info 15 Toolbar 15 1 File Toolbar a B Open Save Open This button opens saved files E Save This button saves images as files 15 2 Edit Toolbar Undo Redo Udno This command restores an activated image to the state before the last executed function was applied om Redo This command does an action which undo was last applied to the activated image 15 3 Mouse Mode Toolbar e amp Select Pan ROI R Selection Mode In this mode mouse could select measurement objects and annotation objects ih y Pan Mode In this mode mouse dragging panning moves the view area of the activated image ROI Mode In this mode user could set the Region Of Interest ROD by mouse dragging 15 4 Zoom Toolbar Ss E A Zoom ln Zoom Out 100 Auto Zoom e Zoom In User could zoom in the activated image step by step with this function e Zoom Out User could zoom out the activated image step by step with this function Normal View This function returns the activated image to the image with the original size window Auto Zoom This function fits the size of the activated image to that of the image 15 5 Acquire Toolbar U e Preview Snap y generates a new window of the image
35. Panasis User Manual Panasis Copyright 2011 Huvitz All rights reserved Index gg nn A 8 AS a 9 A a la 10 A o EIN cet ccs eeep cece eh cece czy case each cae apres aba apes ebeses apes eb E 10 3 2 Open CIO italia 11 33 Cl s e e re 12 A ese 13 ds SAO A ora 14 O tiles 15 3 7 Environmental Option csssessessesessessesssessesussessssussessecussessecucsessecussesseeuesesseeusseeseenssesseeneeeess 16 3 7 1 General Lacio oo aiii 17 3 7 2 A e O toupee statuses aie A 18 3 7 5 HUVITZ Upright Microscope Opti0N oooooonooconconocnonconncnonocnoconononocnoooonoononornocnoonoos 21 3 7 6 Measurement sat ed iO 25 dde Management Service usuarias dina 26 A AREA ee ca ec has 27 Ar EEM ENU O PO O oR Eee EP PCP eee eee ee er ree 28 A Undo CUZ tot it dl lt Dt 28 A2 Redo CEY A 29 Aas COPY ACE sins to OE 30 AA A A O ee ee eee er eee 30 A A a ORES 31 A0 o A a O POE PO III ett neta 31 Ad Select Al CTIA een odas aa dad oia edo Ee ey SE 32 Al ao are ere re rn oe een Ta Oe nro ee EEO NOESEN ONENA 32 E Pan ds 33 4 10 O Yo F S AAA E 33 A A A a al aT te 34 me OO A O A A A 35 e LOMO UE noia rere 36 30 Normal Vie WE I F7 oi a it cats ei 37 at AMO LOOM a os 38 3o GWEN e EE E SE E ARAE N AEAEE 38 IO a lt 3 20 16 9 A NRC OY Ee ON Oe one oN ea 40 e hd 9 2 O ae en en ene nets eet meet een eee eee eee eee eee eee ne eee ne ae 42 A OCCT SPT NCCI SORT Sie TRIN OS CIE RRS 44 S SUIC CEN EWE Peonio erenn 45 591
36. Reset This button sets the crossing point at the center of the image 307200 000000 pixel 20pixel 30pixel It draws square grid or concentric circles with the assigned interval The grid interval and the total area of the image are shown Clock 116h 11m 07s 084ms It shows stopwatch or current time since the camera is connect to the PC Stopwatch shows the time since the camera is connected to the PC which is reset to zero when Panasis is newly run Current time shows the total past time since the camera is connected to the PC The position of the clock is determined by a user Overlay Flatten If this button is checked then the overlay is flatten to the image when snapping an image 6 8 Focus Indicator It show the focus value of the activated image which estimate how much the image is focused If Apply ROI button is not check it shows the focus value of whole image region Otherwise it shows that of ROI If you push the Reset button l then Max value is set to the current focus value After that if there comes a focus value larger than the Max value the Max value changes into the new focus value Focus Indicator Focus rate 30 390 ra Examples of Focus Indicator The following three cases show the focus values for the different focus states of a same sample d Al mx re gt Y Apply ROL out Focused below the focal plane FP On Focus
37. a low pass filter where only low frequency components in the Spatial frequencies of an image pass High pass this command is a high pass filter where only high frequency components in the spatial frequencies of an image pass Sculpt this command uses Emboss filer User sets the size of the filter Morphology Filter Open this command performs Open Morphology operation Close this command performs Close Morphology operation Erode this command performs Erode Morphology operation Dilate this command performs Dilate Morphology operation Tophat this command performs Tophat Morphology operation Blackhat this command performs Blackhat Morphology operation Morphology Gradient this command performs morphology gradient Morphology operation Apply this command applies the chosen operation to the activated image region Note Slide bar controls the zoom in out ratio of the Pre result image which shows the result of the chosen operation 8 2 Brightness Contrast The following image processes are provided If ROI is set then the image processing is applied to ROL All image process in Panasis support Undo Redo Briahtness Contrast Brightness this command changes the average brightness value of the image from 100 to 100 Contrast this command changes the contrast of the image from 100 to 100 Gamma this command changes the Gamma value of the image from 0 0 to 3 0 Inversion this command inverts
38. ale This command changes the activated image into a gray scaled image 74 Flatten If there are measurement objects or annotation objects in the activated image this command creates a new window with an image flattened with the objects The flattened objects cannot be edited no longer 8 Process Menu Mm Filter MW Brightness Contrast NW Hue Saturation M Histogram Equalization m Split E Merge NW Manual Multi focus NW Manual Tiling 8 1 Filter The following image processes are provided If ROI is set then the image processing is applied to ROI All image process in Panasis support Undo Redo Lapladan 1 Sobel Low pass High pass Sculpt Filters consist of spatial filters and morphology filters Spatial Filters Gaussian Blur this command uses a gaussian kernel User sets the sigma value and the size of the kernel Sigma 1 WE Kernel size Median Filter this command uses a median filter User sets the shape and the size of the kernel Style 9 Square Kernel size E EA Sharpen this command uses a sharpening filter User sets the size of the kernel Sobel this command used a Sobel filter and applies it to x y directions both User sets the size of the kernel Kernel size 3x3 9 5x5 7x7 Laplacian this command used DOG filter which is similar to laplacian filter User sets the size of the kernel Low pass this command is
39. already an image added into the workspace then no image is added until the attachment is executed 9 Measurement Menu M Calibration Mi Basic Measurement HM Combination Measurement NW Live Measurement MW Measure Result M Data Collector MW Line Profile Mi Scale Bar 9 1 Calibration Calibration wizard shows an applicable calibration list creates new calibration data and applies the selected calibration data to images Calibration Mame Es Y Defaultipixel 6 7 Cum 0 Cum 10 Cum 12 Cum 15 urn x20 urn 25 kum 30 urn 35 kum 45 kum x6 Her tum Dc Cum 100 Cum 12 Cum Pixel g 520345 um pixel 7 993564 um pixel 6 456755 um pixel R 312656 um pixel 4 310343 um 7 pixel 3 244093 um 7 pixel e 5919396 um pixel 2 1551 um pixel 1 655204 um pixel 1 4476 um pixel 4 220692 um pixel 4 022346 um pixel 3 216296 um pixel e errol um pixel Itai EE BE Pixel 9 528345 um pixel 113935564 um pixel 6 450755 um pixel R 3reb56 um pixel 4 510545 um pixel 3 244095 um pixel e 519399 um pixel e 1551 um pixel 1 655204 um pixel 1 447178 um pixel 4 720692 um pixel d 022346 um pixel 3 216296 um pixel Error um pixel Mn uE Manual This button creates a manual calibration generator Auto This button creates a automatic calibration generator Load This button loads a calibration set from saved
40. and Panasis 3D Profiler Package Before starting Device control connect Panasis to the microscope and register objective lenses and calibarion data as explained in HUVITZ Upright Microscope Device Control Objective lens EE E E 10x 20x 50x 100x The objective lens filled with blue shows the lens used now in the microscope Little squares below lens images show those lenses have IL values Check the AMS check box to apply AMS If AMS works the calibration data registered in HUVITZ Upright Microscope are applied on Live window and Preview Push button to expand the control dialog in order to control a lighting of the microcsope Device Control Objective lens 10x 20x 50x 100x AMS Lighting source FJ 1 Gaye a UP I 0 Hs A ies ECO m Tan i Push lighting button to go back to the compact dialog Check IL check box to use IL function in HUVITZ microscope button saves the current light value to the IL memory in the microscope with the selected objective lens information UP and DN button decide the lighting type whether reflective light or transmitted light to use s a B controls a light Check ECO check box to use ECO function of HUVITZ microscope e i 5 controls the ECO waiting time SIEEP button changes the microscope mode into the ECO mode Note IL is a function which memorized the lighting values for each objective lens
41. ation os en 145 SPSS APpIy Cal lb OA eb od 145 91 Basic Measure mentes rasesasodnsnisenas an isu nasa 146 9 3 Combination Measure Met 1 0 esesesesseseseeseeseesecsecsecsessessesecsecsecsecsscuecucsucsessecseeneeneaueeneeeeseeneenss 158 9 4 Live Measurement Eso odo coa edo 165 93s Meas re TOUS e e E htt eal E ed 166 Oi Data CO e O 168 961 Data Collector Settings sororia Oe 169 97s Hne PE OUI Cesc ces psc actes cutest tate Sec aa cc ted tact tas lta neues tac EE 170 Lor S eo oes E aS ea 173 STG TNIV eye It MNS MI eee RO 175 LOSE PRITAM ACE ONT TOO Si e dd dotadas o ade eb 175 10 1 1 Annotation properties suscuacnssinsninsonenonodnuonsonenosoeoneonosonen nds 176 TAME OS COPE DUION viaccess eles e dd el 179 11 1 Device COmMTOODHON creea io 179 11 2 Z Axis Motor Control Option 0 cc csssssssessesesseesesessesseseeseesesesseesesesseeseeeseeseeneseeneeeesess 182 LEA CO NCE OUD sie id 183 EZ ED ee T 185 A ol 187 T A A A oR 189 A E ER OO II II oh tel ecb pe ETNE 192 12 1 EDO TO EXCO loa A 192 12 2 EXPO CSV e 195 AA A A EST RTS TRC aT ORT SIE eect CIR SOT A 196 13 1 AS CACC ehh eee eae tee 196 1322 Tile Horizontally FTO 20 ccccsesssssesessessesessessessssessesussessessssessecsssesseeseseeseeseseesseseaesseeneeneses 197 13 3 tiles Vertical o dto eer ee eer oo 198 13 4 Close All SHIft FA oo iicccescscscssssssssssssscscsssssssssssssssscsssssvscscssesssesesesssavasasssanaaveeees 199
42. button to start AH Push button to stop the process 7 The measured data is listed in Data collector Note To add the height data to the Data collector 3D Profiler Height item should be added in the collector setting If Top position to set is below the Bottom position set before this position could not be set as Top Set the position as Top after setting the lower position as Bottom If Bottom position to set is beyond the Top position set before this position could not be set as Bottom Set the position as Bottom after setting the higher position as Top 12 Report menu MW Export to Excel M Export csv data 12 1 Export to Excel Panasis saves data from the measurement result and data collector and images as an Excel file format oO on Dion fi wi Ni Aa 7 x 0 38 E E oal o O 037 os 037 os 8721 When Report Export to Excel is clicked the following dialog shows up Export options Option Image Original Image Y Overlay Image Crop Image Measurements V Measurement result Measurement statistics Collect J Collect result Collect statistics Original Image Report includes the original image Overlay Image Report includes the flattened image Crop Image Report includes cropped images After OK button is clicked with Crop Image button checked the following Crop windows appears Crop Image Measurement resul
43. ck the Live image Panasis could restart tracking if you align Live image with the image of the rectangle region of the result window za 30 66 1114144144444 Result Preview Window Window End of Live Tiling When Live tiling if you push the End button Live Tiling ends creating the result image with the images collected by that time Live tiling Tiling E Auto tiling f P120503_ 20 58 A 7 gt ye x aa EAE E E f gt of oc ar SU Aah N TAA M JF 0242CD TAIWAN 2345016000 BBB ISI 11111111141444444444 Note Panasis could miss the image tracking because of the system overload When image tiling processing the rectangle is orange please do not move the sample 6 11 Select TWAIN device It shows the TWAIN device list Please select the TWAIN camera which you want to use Select Source IE TWAIN 2 0 32 32 Imi Tech Camera Twain Driver 1 1 32 32 6 12 Import TWAIN image It runs the camera control program supported by the TWAIN camera chosen in Select TWAIN device menu If you execute the image acquire command in the TWAIN program Panasis generates a new window with the image from the TWAIN program rta lw 50 aja Od Sooo le w 7 Image Menu Mm Resize M Rotate M Grayscale Mm Flatten 7 1 Resize Resize creates a new window with the dimension changed image of the selected image This button is
44. does not change file formats and filename extension gt bD large dia w large dia w 4 tn L Gallery image Changing a name in the Gallery Note It is possible the image multi selection in Gallery by clicking images while pushing the Shift key or the Ctrl key Also it is possible to save or close the multi selected images Since the Gallery window is DockingPane the window could be attached anywhere When the window is overlapped to other DockingPane each DockingPane is distinguished by Tab 5 6 Histogram Histogram window shows the RGB Intensity and HSV histogram of the activated image Histogram ORGII IR Mic Ya Mr Deasy OH Os Dv C Excluded saturate points Use ROI RGBI This check box turns on off R G B and I histograms A This check box turns on off R histogram G This check box turns on off G histogram B This check box turns on off B histogram This check box turns on off I histogram H This check box turns on off H S and V histograms H This check box turns on off H histogram S This check box turns on off S histogram V This check box turns on off V histogram Exclude saturated points This check box turns on off the exclusion option which decides whether Panasis discards the data whose intensities are bigger than 255 or not Use ROI This check box turns on off the ROI option which decides whether Panasis applies histogram
45. e Ctrl N New Image generates a new empty image window The default file format is set to Bitmap Before generating a window it is possible to fix the size and color model of the image The size of the image is set to 640x480 but the size of the image is set to the size of ROI when executing New function after copying an image holding ROI New Image Color model RGB 24 Width a Cancel Height Color model select the color model of the image width set the width of the image height set the height of the image This button generates a new image as setted Cancel This button cancels New Image 3 2 Open Ctrl 0 Open loads image files from data storages The default path of Open is WWMyDocumentWHuvits PanasisImages Panasis can read the following types of images TIF BMP PNG JPG E ns Lookin CG Images COIE G Histogram Equalization l O Multifocus My Recent E Surface View Documents S Tilin 3 Blohs png checker png Finebrane png Kimberly png My D ocuments My Computer File name My Network Files of type All image files Ca Note Panasis memorizes the last used directory 3 3 Close Ctrl F4 Close closes the activated window When needed to save the image it appears the dialog asking whether you save the image or not Close 06 i p ng r Would you save the image wot cono This button saves the image This button do
46. e device Properties UVC Video Controls UWE Special Controls Camera Control ideo Proc Amp Gain Y la F Auto Exposure 1 32 sec F Auto Auto Reference 255 F fijesec Auto WihiteBalance Auto White Balance Red lez E White Balance Green leq F white Balance Blue HH leq 7 Color Enhancement Enable Auto Max Value 3 Put it on the focal position of camera a piece of white paper or a sample for the white balance set up 4 Adjust shutter control or turn on auto shutter mode to make a camera image have the proper brightness 5 Push the One Push button 6 Push the OK button Note It is recommended to set the camera shutter mode on Auto mode when focus ratio or lighting condition changes 6 2 Preview F5 Preview shows a live image of the camera Preview window has the following properties e Its image is not listed in the image gallery e Its image is not shown in the image navigator e Overlay Focus Indicator and snap are available e It has the same calibration data that Live measurement window has Preview 28 38 6 3 Snap Space Snap captures an image from preview window or Live measurement window and generates a new window of the image E C 1207271 35 62 6 4 HDR Capture HDR Capture creates a new image from a bright image to a dark image of a sample to show all regions of the sample well in spite
47. ecked Panasis initialize COM Port automatically whenever Panasis Starts 2 Registration of objective lens and calibration set decides the order of objective lenses in Device Control To register ojbective lenses push Lo or buttons Then the the following dialog window appears Select lens After selecting the magnification of an objective lens push the Select button If there is no lens in the above list please push New button to add a new lens to the list Append lens Input the magnification of the lens and Push the Append button Register a Calibration set for applying to AMS Default pixel When no calibration datum is registered button calibration button appears When a calibration datum is registered the calibration datum name appears x100 um as button calibration button To generate new calibration data or edit the data push a calibration button Select calibration Mame Default pixel x5 um x10 um x20 um x50 um x100 um To create a new calibration datum push Note This function is provided in Panasis Upright and Panasis 3D Profiler 3 7 6 Measurement You can change the measurement configurations Preference Measurement General Camera Shortcut Display option a ini 2 Label and vaule both Label only Management Service E Value only None Decimal digit Display option option for displaying labels and meas
48. ed image is larger than the window size 4 10 ROI Mode F3 In this mode user could set the Region Of Interest ROI by mouse dragging If ROI is set the image processing works on the area 5 View Menu Zoom in Zoom out Normal View 1 1 F7 Auto zoom F8 Gallery Histogram Navigator Tab Surface Viewer 5 1 Zoom In User could zoom in the activated image step by step with this function Maximal magnification is 2500 Mouse wheel up also zooms in the image by one step If the zoomed image is larger than the image window vertical and horizontal scroll bar are automatically created 5 2 Z00M out User could zoom out the activated image step by step with this function Minimal magnification is 1 Mouse wheel down also zooms out the image by one step 5 3 Normal View 1 1 F7 This function returns the activated image to the image of its original size 5 4 Auto Zoom F8 This function fits the size of the activated image to that of the image window 5 5 Gallery Gallery shows the opened images as thumbnail images User could activate an image in Gallery by clicking the image If mouse right button is clicked it shows up the pop up menu where user could change the name of the image and save the image or close the window If mouse left button is clicked once more while the image is already selected user could change the name of the image as in Windows However this operation
49. ent Measurement Management Service Snap Set preview window as full screen Draw 45 Vertical Horizontal line Draw a square 3 74 Layout Layout manages the layout of Panasis Preference Layout General Camera C Fixed Layout Shortcut Layout Toolbar text HUVITZ Upright Microscope Measurement Management Service Layout Management Load layout Save layout Default layout Fixed Layout If this button is checked Panasis fixes the layout to prevent layout change Toolbar text If this button is checked Panasis shows texts in a toolbar menu Load layout If this button is checked Panasis loads a saved screen layout from files Save layout If this button is checked Panasis save the current layout to a file Default layout If this button is checked Panasis changes the screen layout into the default layout 3 7 5 HUVITZ Upright Microscope Option In HUVITZ Upright Microscope a user could initialize COM port to control a Huvitz upright microscope and registrate objective lens and a calibration set to apply AMS Auto Magnification Sensor Preference HUVITZ Upright Microscope General Shortcut layout om E F Connect on startup HUVIT Upright Microscope Measurement Objective lens AMS Management Service Default pixel Default pixel Default pixel Default pixel Default pixel 1 COM Port Initialization To control a Huvitz upright microscope the microscope must be con
50. er E lolo x J El P120725 1 100 16 3D Open 2D Open 3D Save 2D Capture Option 3D view 3D profile 4 686 8 800 12 000 000GA um 4 900 8 000 12 000 5 9 5 Option Surface Viewer provides several options to help sample investigation 3D Viewer Option 4 2D pseudocolor contour v Use light Pitch Rainbow Rainbow Monochrome Red Green Blue Cyan Magenta Yellow Gold White Yaw 340 1 000 um E 1 000 um M 6 500 6 566 Uae 000 9 099 115 000 um 15 000 um up 1 250 i 11 250 Tg o E A TA EE st A 3 750 X 0 000 u 009 8 000 12 000 16 000 20 000 um 170 000 u 090 8 000 12 000 16 000 20 000 u 08 2D pseudocolor contour Panasis paints the 3D image with specified colors which are defined differently according to the height 1 000 um gt R 0 500 Ma Alo 000 Be 08 um u t 000 8 000 12 000 16 000 70 900 um u 600 8 000 12 000 16 000 Use Light Panasis gives lighting effect to generate shadows of the 3D image The shadows are useful to surface analysis of the sample Pitch This bar controls the latitude of the lighting Yaw This bar controls the longitude of the lighting 5 10 3D Measurement option 3D Measurement measures heights lengths area volume and et al on a 3D image synthesized by EDF This function is provided in Panasis 3D Profiler If 3D
51. es not save the image Cancel C This button cancels Close Note To close all the windows press Shift F4 3 4 Save Ctrl 5 Save saves the activated image when saving the current file format is conserved For the case of the images not carrying file formats such as captured images this function acts like Save as Note Scale bar Measuring objects or Annotation objects are saved for the TIF images Otherwise they are not saved 3 5 Save As Save As saves the activated image with the name changed It is possible to select the file format to save from the save dialog Panasis can save the following file formats TIF BMP PNG JPG Save As AA saven images E O 5 Histogram Equalization 3 Multifacus My Recent E Surface View Documents Tiling Blobs png LS checker png Desktop 2 FineGrane png 0 Kimberly png My Documents eL My Computer File name Blobs Gave ae a My Network Save as type Portable Network Graphics pra Ba Cancel Note Scale bar Measuring objects or Annotation objects are saved for the TIF images Otherwise they are not saved 3 6 Crop Crop saves the selected ROI and generates a new window with the ROI image If ROI is not created the function is deactivated Panasis supports the following file formats TIF BMP PNG JPG Crop O S Saven Omas OM ES 5 Histogram Equalization k O Multifocus My Recent E Sur
52. face View Documents E Tilin q La Desktop May Documents os hy Computer a File name A120727_1 ALA etmork Save as type Windows Bitmap bmp Save Cancel Note Scale bar Measuring objects or Annotation objects are saved for the TIF images Otherwise they are not saved 3 Environmental Option Environmental Option sets the configurations of Panasis If you run the File Environmental Option the following password dialog appears The initial password is set to 0000 You could change the password in Environmental Option Management Service 3 7 1 General General creates the general configuration of Panasis Preference General Show guide line Open images as fitted to window HUVITZ Upright Microscope Measurement Management Service Set live measurement window to the top when capturing Save as a flattened image Language 3 2 Camera You can register cameras from a camera list Unregistered camera does not appear in Acquire Camera Properties Preference Camera General Camera list LUSIS HUVITZ Upright Microscope Crevis Measurement IMI Tech Management Service E The Imaging Source Lumenera Motic WDM Direct Show L Dummy fake 3 7 3 Shortcut Shortcut shows the shortcut menu of Panasis You can change the listed shortcut keys from ver 2 0 Preference Shortcut Preview HUVITZ Upright Microscope Live Measurem
53. files Save This button saves the current calibration set to a file Delete This button deletes a selected calibration datum UP This button moves up a selected calibration datum in the calibration list DN This button moves down a selected calibration datum in the calibration list Apply This button applies the selected calibration datum to images 91 1 New Calibration This function is used for generating a new calibration Manual DEE Drove 1 When new Calibration dialog shows up the activated image is drawn in the main screen If Open button is clicked a saved image is loaded 2 Draw the calibration line Vertical horizontal or 45 degree inclined lines are drawn when drawing lines with pushing a shift key 3 After inserting calibration information name length unit please click OK button 4 You could set the calibration ratio of x y if you check the X Y ratio box 5 Newly created calibration datum is now listed in the calibration set Auto oT O aN F Show guide line Load Image Calibration Info Scale Unit r i ME 1 When new Calibration dialog shows up the activated image is drawn in the main screen If Open button is clicked a saved image is loaded 2 Panasis automatically finds the calibration bars 2 After inserting calibration information name length unit please click OK button 3 You could set the calibration ratio of x y if you check
54. ically finds out the nearest 3D Profiler Profile Measurement Fj coin h3d Profile Graph 510 20 498 88 487 56 476 24 464 91 intersection point of the threshold line and the profile line from the point where left button was clicked x When there seems to be only one measurement line that means two line are overlaped Drag and set the measurement line to seperate the lines x Even after measurement lines are both generated user could change the threshold Please push right button to exit the measurement tool mode The measured results are listed on the Data window ds 2D View 3D View T Pseudo 2 Scale Lighting Pitch Height Radius Height Length Surface le Angle C S Area 49 06 um 26242um 28623um 0 19 radian lt Profile measurement length 2 vertical gt 3D Profiler Profile Measurement LG_LCD h3d 3D Image Image Option Profile Tool Profile Option sy 2D View 3D View Horizontal Yertical 2 Points Axis me Color Mesh Pseudo la 0 Measurement Tool Z Scale 1 0 X LA Lighti ighting Length Area Curvature Width Pitch ESPAI van ql m 104 371 Measured Data Width Height Length Surface le Angle C S Area Radius 74 51 um Statistics 148 439 Profile Graph 16 08 4 0 00 lt Profile measurement area peak gt 5 10 2 1 2 Profile option User could set the configurati
55. ight map When you do not change the moving direction of microscope tube an height map image Is more accurate 6 10 Live Tiling Live Tiling generates a tiled image from live images of Preview window in real time The overlapped region of images is seamless by the Panasis image processing Live Tiling is available when Preview window or Live measurement window is activated Live Tiling Starts Live tiling Tiling Linda Auto tiling Auto Tiling Under the following conditions are satisfied Panasis automatically tiles an image 1 When the small rectangle in Preview window approaches to the end of Preview window 2 When Panasis succeeds to find the last tracked image position after the image tracking failed 3 When the specific points of an image for image tracking moves a lot Add When auto tiling is unchecked user manually tiles images pushing Add button Undo Undo undoes the last tiling process User can continue to do undo processes After undo is applied auto tiling is deactivated During Live Tiling When image tracking process succeeds the larger and smaller rectangle in Preview window is yellow colored When image tracking process fails the larger and smaller rectangle in Preview window is red colored Preview 28 38 Preview 28 38 Success Failure E P120503_1 56 85 m Result Window When Panasis fails to tra
56. ing threhold condition is measured 5 Specify the region with the region tool to measure volume and surface area e When a region tool buttion is clicked a region could be specified e When a region is specified the result data is listed on Data window e Whenever a region button is clicked a region is created 3D Profiler Yolume Surface by Profile coin h3d la L 2D Image Image Option Profile Tool XY 3D yi 2 Points vertical 2D view 3D View Horizontal m E Region Tool Mesh Pseudo Rectangle Z Scale Threshold Option Lighting TNX Pitch Above Below TEN Measurement Option Measured Data Collect Delete Volume Surface area Area Surface area Area 22 Rectangle 378805 41 um 14572 34 um 14285 84 um 1 02 Statistics 23 Rectangle 391785 56 um 17314 39 um 16889 23 um 1 03 Profile Graph 510 20 498 88 487 56 464 91 453 59 lt Volume Surface by Profile 2D View gt 3D Profiler 3D Image Yolume Surface by Profile coin h3d mesm Profile Tool pog Horizontal Vertical 2 Points Image Option 2D SL 2D View 3D View M Color Mesh Region Tool Rectangle Threshold Option Measurement Option LJ Peak Valley 276 150 Measured Data 138 075 Collect Delete Volume Surface area Area Surface area Area AR i 23 Rectangle 908165 25 um 45769
57. ion The height difference between 2 Points two specified points is measured A reference point is specified first The height differences between the point and newly added points are measured i Reference 3 Select the method to specify points on an image in the measurement tool The clicked point on the image Point defines the input point The center point of a rectangle Rectangle created by a user defines the Input point 4 Specify the points to measure height differences e Height Difference works in 2D View e The points used in the measurement are linked by lines e The result data is displayed in the Data window e The reference point does not have a height data e Please push right button to end a measurement 3D Profiler Height Difference J LG_LCD h3d 2D Image 2D View Pseudo Z Scale Lighting Pitch 3D View Point Rectangle ME Mesh 0 Compare Option A D 2 Points Reference Z Height Width 57 12 um 47 38 um 9 74 um 27 53 um 7280 um 6719 um 5 61 um 52 96 um 35 33 um 41 60 um 6 27 um 13 59 um 74 95 um 97 40 um 22 45 um 27 11 um 152 70 um 123 97 um 28 72 um 48 67 um 167 06 um 14 36 um 24 60 um 166 73 um 14 03 um 18 07 um 145 60 um 7 10 um 16 32 um 128 60 um 24 10 um 24 75 um 124 97 um 27 73 um 27 99 um 5 10 2 2 2 Height by Profile Height by Profile measures heights on the profile line 1 Select Height by
58. ion on or flatten the image using Flatten in the Image menu Note Since the basic measurement window is DockingPane the window could be attached anywhere When the window is overlapped to other DockingPane each DockingPane is distinguished by Tab 9 3 Combination measurement Combination measurements measure geometric relations between objects basic measurement objects and combination measurement objects both When objects are selected and they could compose combination measurements then the corresponding combination measurement buttons are activated In general combination measurements are completed by clicking the buttons Users cannot deform the combination measurement objects but the objects automatically change when the element objects are changed If a user copies a combination measurement object only then the copied object pretends to be a basic measurement object If a user copies a combination measurement object with its element objects then their links are still maintained in the copied objects Combination measurement provides the following methods Combinatio 9 Combination Position There are three options center position division position node position This button is activated when at least one of above options is available It has data the same as Point has Center position This button is activated when only objects having center points are selected In the case of
59. ion when moving when moving a newly added image Panasis compensates the position of the image to make the overlapped region natural Fix position when attaching when attaching a newly added image Panasis compensates the position of the image to make the overlapped region natural Undo Undo can cancel the image attachment process step by step If the last attached image is from a camera then the image disappears If not the image goes back to the gallery Gallery The images from files are listed here By double clicking or dragging an image you could add the image into the workspace Workspace Workspace is where the tiling process is working The image added here could be moved to the proper position by dragging Double click or enter attaches an image into the result image Zoom In Out Moving a mouse wheel or clicking the zoom ration button makes the workspace 1 1 1 2 1 4 1 8 scaled size Cancel This button cancels the current manual tiling process and Panasis goes back to the main screen Complete This button ends the current manual tiling process Then the following crop window appears Manual Ting Completion When the complete button is clicked the final image is generated in a new window P120727_19 55 04 f r Lyd ently y _ l Note Only one image could be added into the workspace at once Therefore if there is
60. is same as specifying a rectangle The result data are width and height Count This object measures the number of points created by clicks The number of counting points are not fixed The result datum is the number of counted points Properties general The properties of basic measurement objects are set here When an image is loaded or captured this page works If no object is selected the property is applied to all objects created after the property changes If any objects are selected then the property is applied to the ones If the Apply button is clicked the property is applied without closing property dialog Property Line Width Line Color Font Font Size Bold Oblique Underline Properties Angle The properties of angles are set here When an image is loaded or captured this page works The application policy is same as that of general properties Property Cirde Relation 1 8 Internal angle Exterior angle Properties Circle The properties of circles are set here When an image is loaded or captured this page works The application policy is same as that of general properties Property Object Angle Circle Relation 4 Properties Relation object The properties of combination measurement objects are set here When an image is loaded or captured this page works The application policy is same as that of general properties Property
61. istance between two horizontal lines line This object measures the length of a line which are created by specifying two points start point and end point by mouse clicking If you keep pushing a shift key when drawing a line then the line is allowed to have the following degrees of inclinations only 0 45 90 135 180 225 270 315 The result data are the length the position of the middle point x y position of the start point and the end point the angle between the line and x axis FA LN 1 175 66 pixel Polyline The number of point to create a polyline is not fixed Dragging during creating a polyline adds them to the polyline all points where mouse moves A polyline has total length and the x y position of the middle point as data Rectangle This object measures the area of a rectangle which are generated by specifying two points upper left and bottom right Keep pushing a shift key when drawing a rectangle generate a square The result data are the area circumference length x y position of the middle point RT 3 20655 86 pixeF Polygon The number of point to create a polygon is not fixed Dragging during creating a polygon adds them to the polygon all points where mouse moves A polygon has the area circumference length and the x y position of the middle point as data r P P y EA A o MA o d W OL a 2 point circle This object is created by specifying two p
62. ked 4 Set Memory Push L tM botton to memorize the current postion then the tag of M appears in the Navigator 5 Set BL Bottom Limit Push sete button to set the current position as the BL then the tag of BL appears in the Navigator Push Gear BL button to clear the BL then the tag of BL disappears When BL is set the head could not move down below the BL 11 2 2 EDF EDF captures images scanning along z range set by a user and synthesizes a 3 dimensional image EDF When _EDF button is clicked EDF menu appears under the z controller E Absolute LI s T Show Surface viewer 1 Move the microscope head head to the top position of the sample using z controller 2 Push button of Top to set the current position as the upper limit of the head movement This position is shown as T in the Navigator 3 Move the microscope head head to the lowest position of the sample using z controller 4 Push button of Bottom to set the current position as the low limit of the head movement This position is shown as B in the Navigator 5 Set the number of capture images in the Step box The more capture image is the more accurate image is but the more time it takes Panasis recommends 20 30 images 6 Push button to start EDF Push button to stop the process If Show Surface Viewer is checked Panasis screen changes into 3D Measurement after the EDF process Note If
63. key Shift key or mouse drag lt Remove This button moves images chosen in Selected Frame into Available Images This button is activated when an image in Selected Frames chosen You could move multiple images with Ctrl key Shift key or mouse drag a Remove All This button moves all images chosen in Selected Frame into Available Images This button is activated when there is at least one image in Selected Frame 3 Topdown if this button is shown Panasis treats the first image in Selected Frame list as the first image Bottom up if this button is shown Panasis treats the first image in Selected Frame list as the last image ON lUP DN movement This button moves the selected image up and down in the Selected Frame list This button is activated when an image in Selected Frame is chosen Start Execute Manual Multi focus During multi focusing the following progress bar shows up If you push the cancel button the process will stop There are the option list for multi focusing Focus analysis options Max Local Contrast This method considers areas with high maximum contrast to be in focus Pixels are analyzed in the area around the current pixel location The pixel from the plane with the largest variance or local contrast is selected Max Stack Contrast The mean value of the pixels is calculated The pixel from the plane with the largest variance from the mean is
64. m The data copied to Clip board could be attached to an image by Paste function Note Only for the image data not measurement objects and annotation objects it is possible to copy the data to Window programs 4 5 Paste Ctrl V This function attaches the data in Clip board brought by Copy and Cut to the activated window Since the data still remains after pasting it is possible to paste the same data several times Note It is possible to paste the data in the Window Clip board brought by Windows system to images 4 6 Delete Del This function deletes the selected measurement objects and annotation objects in the activaed window 4 7 Select All Ctrl A This function selects the selected measurement objects annotation objects and scale bar or image data in the activated window When mouse mode is Select mode Select All makes all measurement objects and annotation objects selected state When mouse mode is ROI mode Select All sets the ROI region to the whole image 4 8 Select mode F1 Select Mode is the default mouse mode In this mode mouse could select measurement objects and annotation objects Click Ctrl Click Dragging the last two are used for multi selection are used to select objects Their operations are same as they are in Windows system 4 9 Pan Mode F2 In this mode mouse dragging panning moves the view region of the activated image Panning works when the activat
65. nected to pc by COM port 1 Combine an RS232 cable and USB converter which are involved in Panasis PKG 2 Connect the RS232 cable to an RS232 port backside of the microscope and USB converter to user s pc In case of 3D Profiler connect RS232 cable to the RS232 port backside of a 3D Profiler control box 3 open File Environemntal Option HUVITZ Upright Microscope page an koe E available ports should be upto two Otherwise please check the cable connections If there select available port If there is no problem to the step 3 the number of is no problem in the cable connection please install usb converter driver in the CD involved in the usb converter package Connect button 5 Intialize COM Port by pushing If COM Port is initialized the following dialog window appears hvlOControl A COM port initialized If not the following dialog windows appear hvlOControl Failed to open the serial port COM1 115200bps yf ERRORCODE 2 hvlOControl A COM port initialize failed A If COM port could not be initialized when there is no problem to the step 4 please check the power switch of the microscope If power switch is OK but COM Port is still not initialized turn off the microscope and 3D profiler control box and shut down user s pc Then turn on the microscope and 3D Profiler control box Restart Panasis Try COM Port initialization several times 6 If Y Connect on startup is ch
66. nerated RGB Split When this option is selected Panasis splits a image into three gray scaled images 8 bit each are corresponding to R red G green B blue channel images If Maintain Channel Color is checked Panasis gives colors to the result images This does not mean the result images have real colors They are just shown colored Split image Gagan Prewe or mode RGB HS Jl Mantan Channel Color HSV Split When this option is selected Panasis splits a image into three gray scaled images 8 bit each are corresponding to H hue S saturation V value channel images If Maintain Channel Color is checked Panasis gives colors to the result images This does not mean the result images have real colors They are just shown colored Split Image ee ee Previer Mantain Channel Color 3 6 Merge This command merges three one channel images 8bit to create one thee channel image 24bit Panasis supports RGB HSV color models for merging Merge Image list Available images Selected frame P120727 2 P120727_3 P120727 4 Color model 8 RGB E HSV Cancel Available Images this list shows all opened 8bit images If there is an image in the Selected Frame the Available Images list is filtered by the image size of the image Selected Frame this list shows all selected images for merging Up to three images the list could contain If three image
67. not activated when an image is not open Resize Image size ean mar Distortion Permit Image quality Interpolation nearest neighbor ESSE Reset This button restores the default setting original size ok OK This button executes the Resize Cancel cancel This button cancels the Resize and closed the dialog 1 Image size Please input the size of image you want Panasis provides two units pixels and the ratio to the original size 2 Distortion Permit This check box decides whether the ratio of the width to the height are kept after resizing 3 Interpolation This combo box selects the interpolation method in the followings nearest neighbor nearest neighbor interpolation bilinear bilinear interpolation area area interpolation cubic cubic function interpolation The lower method goes the better the image quality is and the longer time the process takes Note Resize keeps the original image 7 2 Rotate This menu provides image rotations and image flips 90 Clockwise 90 Counterclockwise Vertical Flip This button flips an image vertically Horizontal Flip This button flips an image horizontally 90 degree Clockwise This button rotate an image clockwise by 90 90 degree Counterclockwise This button rotate an image counterclockwise by 90 Note Rotate does not concern ROL After Rotate is applied zoom magnification is set to 100 7 3 Graysc
68. oints a center point and a point on the circumference It has a label with a radius or a diameter The result data are the radius diameter area circumference length x y position of the center point 2 CR 5 102 51 pixel 3 point circle This object is created by specifying three points on the circumference It has a label with a radius or a diameter The result data are the radius diameter area circumference length x y position of the center point x CR 6 104 12 pixel Ellipse This object is created by specifying three points on the circumference It has a label with the length of major axis or minor axis The result data are the length of the major axis the length of the minor axis area circumference length x y position of the center point EL 1 142 14 81 43 bs Arc This object measures the length of an arc which are created by specifying three points on the arc The result data are the length the radius the diameter x y position of the center point Angle This object measures the angle between two lines which are created by specifying three points an intersection point and two other points for defining two lines The result datum is an angle E r ANG 3 40 59 deg X Y width This object measures the width and the height between two vertical lines and two horizontal lines which are created by specifying two points of two opposite corners which
69. on is possilbe select objects holding a control key pressed 3 Select a measurement tool in the measurement tool box and measure a profile Measurement tool Description This measures width height length and angle between x axis K 2 vertical 1 Vertical The intersections between a profile line and a Length measurement line are used as measurement points 2 vertical 2 horizontal A line Measurements are excuted regardless of a profile line This measures an area of the region defined by the relations between a profile line and the user define rectangle i peak This measures the area defined Area by the intersection between a profile line and a rectangle drawn by a user valley This measures the area of the region which is inside of a rectangle drawn by a user and outside of a profile line This measures an angle between intersection points of measurement lines and a profile line Angle A 9 j 3 point angle This measures an angle between three intersection points of measurement lines and a profile line This measures a curvature of the profile line using Curvature three intersection points of measurement lines and a profile line This measures the width between two intersection points of a horizontal threshold line and a profile line E pa Auto Width Set the threshold by dragging the horizontal threshold line Measure the width using vertical measurement lines Panasis automat
70. ons of axis interval and range in Profile option Axis Config Max Y 16 0761 um 1 1 Min Y l um Interval of Auto um Auto Cancel Max Y this sets the maximum Y height value of the profile graph to show Min Y this sets the minimum Y height value of the profile graph to show Interval of Y this sets the Y height axis interval on the profile graph e Auto Panasis automatically sets the Y axis interval 1 1 this sets the ratio of X axis to Y axis to 1 1 Default Setting this button resets the configurations of axises back to the default setting this button applies the configurations to the profile graph this button closes the Axis Config dialog without applying the configurations Note In the default setting the maximum value of a profile line is same as the Max Y and the minimum value of a profile line is same as the Min Y 5 10 2 2 Height Measurement Height measurement measures the height differences between specified two points or several points Panasis provides the following three measurement themes for height measurement 1 Height Measurement image 2 Height Measurement Profile 3 Height Measurement Histogram 5 10 2 2 1 Height Measurement Image Height Measurement Image measures the height difference between points which are specified on an image 1 Select Height Measurement Image from Measurement theme 2 Select an option in the compare opt
71. opy Cut Paste Delete of measuring objects annotation objects and images Image processing image rotation Note Multiple Undo applications are allowed 4 2 Redo Ctri Y To the activated image this command does an action which undo was last applied to The functions which Redo could be applied to are the followings Addition Edition Delete of measuring objects and annotation objects ROI Copy Cut Paste Delete of measuring objects annotation objects and images Image processing image rotation Note Multiple Redo applications are allowed 4 3 Copy Ctrl C This function copies image data or selected measurement objects and annotation objects of the activated image When mouse mode is Select mode Copy copies selected measurement objects and annotation objects to Clip board When mouse mode is ROI mode Copy copies the image data in ROI to Clip board The data copied to Clip board could be attached to an image by Paste function Note Only for the image data not measurement objects and annotation objects it is possible to copy the data to Window programs 4 4 Cut Ctrl X This function cuts image data or selected measurement objects and annotation objects from the activated image When mouse mode is Select mode Cut copies selected measurement objects and annotation objects to Clip board and delete them When mouse mode is ROI mode Cut copies image data of ROI to Clip board and delete the
72. point the button is activated when more than two points are selected This button measures the centroid of selected objects Division point This button is activated when only one object having length such as line polyline rectangle is selected The number under the button means the number of division User could assign the starting division point for rectangle circle and ellipse TAE sok AE o a 2i arr 8 30842 156 292 fit SHA PY Ms E N i EONA AA E Os E ARAS A a eal gt a RT ag ate ae Be ya i de E Ths SIC E ad aX FS IPT a 500 52 249 217 PT 7 36450 20890 een SS Node position This button is activated when a line and a line or a line and a polyline or a line and a polygon or a line and a circle or a line and a arc are selected This finds a point where a line meets the other selected object PT 4 209 64 492 12 y E Combination distance There are four options center to center Max Min Perpendicular distances This button is activated when at least one of above options is available It has data the same as line has Combination Distance Max Distance Min Distance Center to Center Distance This button is activated when two objects having centers are selected It measures the distance between two centers of the objects GR 1 84 48 pixel DT 3 174 10 pixel as LN 2 197 33 pixel Max distance Min distance These
73. r png 100 25 EY Savd S Save As A amp Close Q Close AlI A 5 9 Surface Viewer This command shows a 3 dimensional image and saves it loaded from 2 dimensional images or a 3 dimensional image E __ Surface Viewer 3D Open 2D Open i 3D Save 2D Capture Option 1 000 u 1 0 500 HA 0 000 3D Open 2D Open 3D Save 2D Capture Option 5 9 1 3D Open This command loads a 3 dimensional image which has a Panasis own file extension p3d These images does not need to set the height width height information which are needed when Panasis opens them as 2D Open needs 3D Load em om Look in O Surface View ay amp g E gear p3d Ed LareqWheel del El LaregWheel p My Recent E small wheel p3d Documents 13 Desktop My Documents PE My Computer a File name My M etwork Files of type Panasis 3D File pad m Cancel Surface Viewer 3D Open 2D Open 3D Save i 1 000 u Note 3D files are generated by 3D Save 5 9 2 2D Open This function shows a 3D image from 2D images Max Height 1 0 Min Height 0 0 StartX pos 0 0 Start Y pos 0 0 X Range 20 0 YRange 15 0 m _ C Wusers panasis Documer Panasis images WiSurface Scale unit Micrometer 1 Panasis Wimages Y Surface View LargeWheelT opo bmp View WLargeWheelT exture bmj Smooth very slow due to bilate
74. rial filtering oe Load Height map This button loads an image as a height information Panasis recognizes the gray scaled image as a height information Height map could be generated by checking the topographic map check box in Manual multifocus Load Color map This button selects a image as a texture image Max Min Height User inputs the maximum height and the minimum height in the image Start X pos X Range User inputs the minimum x position of the image and the x range of the image Start Y pos Y Range User inputs the minimum y position of the image and the y range of the image Scale unit User selects the scale unit used in the image Smooth This button decides how to generate 3D image If this button is checked the quality of 3D image is enhanced but it takes long Open This button creates 3D image as set above Cancel This button cancels Surface Viewer 5 9 3 3D Save This command saves the current 3D image as Panasis own 3D file format p3d Surface Viewer Lo E e 3D Open D Open 2D Capture 3D Filter 30 Save Save in C Surface View E gear p d E LaregWheel p3d My Recent E small_wheel p3d Documents Desktop My Documents as My Computer a File name Y My Network Save as type Panasis 30 File p3d Ea Cancel 5 9 4 2D Capture This command captures the image shown in the Surface Viewer window as a 2D image la Surface View
75. s are selected then OK button is activated Element shows the specific channels Add All This button moves all images in Available Images into Selected Frame This button is always activated If there are three images in the Selected Frame this button is deactivated Add This button moves images chosen in the Available Images into the Selected Frame This button is activated when an image in Available Images is chosen You could move multiple images with Ctrl key Shift key or mouse drag If there are three images in the Selected Frame this button is deactivated lt Remove This button moves images chosen in Selected Frame into Available Images This button is activated when an image in Selected Frames chosen You could move multiple images with Ctrl key Shift key or mouse drag lt lt E E Remove All this button moves all images chosen in Selected Frame into Available Images This button is activated when there is at least one image in Selected Frame iTopdown if this button is shown Panasis treats the first image in Selected Frame list as the first channel element Bottom up if this button is shown Panasis treats the first image in Selected Frame list as the last channel element on UP DN movement This button moves the selected image up and down in the Selected Frame list This button is activated when an image in Selected Frame is chosen Image merging result
76. s in the lie profile window If the mouse hits the guide lines mouse cursor changes into a horizontal arrow Then user could move the guide line by mouse dragging Line profile Live measurement Color G B I n t e n 3 i t Y 300 Distance pixel Color This check box turns on off channel profile graphs R This check box turns on off the red channel profile graph G This check box turns on off the green channel profile graph B This check box turns on off the blue channel profile graph I This check box turns on off the gray channel profile graph Info This box shows the profile information of the selected channel between cyan and pink lines X1 the X position of cyan line Y1 the Y position of cyan line X2 the X position of pink line Y2 the Y position of pink line CM Y1 Y2 Y1 Y2 Y1 Y2 Gray channel value Save Data This button saves the profile data as a csv file Save Image This button saves the current line profile image as a bmp file 9 8 Scale bar Scale bar changes when calibration data or calibration unit changes Scale bar could be moved by dragging Also the length of scale bar changes when user drags the end of the scale bar Scalebar Horizontal Text size 12 le Color Thickness 1 x Division Norn Position 8 Upper left Upper right E Lower left C Lower right Horizontal If this box is checked Panasis shows a
77. selected Pixel pixel Pixel pixel Nanometer nm Micrometer um Centimeter cm Meter m Inch inch Mil rit 9 2 Basic Measurement Mouse cursor is different for each measurement item Measurement objects are created by left click and they could be created in succession after the end of an object creation In the case of that it is fixed the number of points which consist of an object right click cancels the creation of the object and Panasis gets back to selection mode where the shape of the mouse cursor Is an arrow Otherwise e g counting right click complete the object creation and Panasis continues object creation mode All procedures of creating deleting and editing for measurement objects supports Undo Redo function Also You can copy and paste them The followings are basic measurement tools Basic Meas YES point This object measures the position of a chosen pixel By one click a point object is created The datum of a point consists of the positions of x and y 4 Y Distance between vertical lines this object measures the distance between two vertical lines which are created by one click for each line The result datum is the distance between two vertical lines W 2 107 91 pixel Distance between horizontal lines this object measures the distance between two horizontal lines which are created by one click for each line The result datum is the d
78. t Report includes the measurement data Measurement statistics Report includes the statistics data Collect result Report includes collected measurement data Collect statistics Report includes the statistics data of collected data When OK button is clicked the following Excel exporting window shows up Excel export Excel document Export target Document Bookt Sheet Sheet 3 nens Jl Open This button opens the already saved file New This button creates a new Excel file Document This box selects an Excel file among the opened Excel files Sheet This box selects a sheet in the sheets of the selected document New Sheet This button creates a new sheet Export This button starts to export data to an Excel file Note If a name is written in the blank next to New Sheet button then a sheet the the name is created 12 2 Export csv data This function saves data from the measurement result and data collector as a csv file Export options Option Measurements Vv Measurement result Measurement statistics Collect _ Collect result Collect statistics ETE Measurement result Report includes the measurement data Measurement statistics Report includes the statistics data Collect result Report includes collected measurement data Collect statistics Report includes the statistics data of collected data OK This button exports the selected data to a csv
79. te 5 10 2 3D Measurement Panasis provides the follwoing measurement themes 1 Profile Measurement 2 Height Measurement 3 Volume Surfcae Measurement 5 10 2 1 Profile Measurement Profile Measurement measures lengths areas angles and curvatures on a profile graph a curved line that describes the surface of a sample 1 Profile Measurement 2 Profile Option 5 10 2 1 1 Profile Measurement When a user draws a featue on an image using profile tools a profile line the cross section line cut by the feature appears in the profile window Profile measurement measures lengths areas angles curvatures and auto widthes on the profile line 1 Select Profile Measurement on Measurement Theme 2 Select a profile tool and draw a feature to create a profile line Place a horizontal line on a specified Horizontal line Bi o position to get a profile line Click two points to generate a line for El Place a vertical line on a specified Vertical line o o position to get a profile line Line Pp a profile line e Whenever a profile tool button is pushed Panasis generates a new feature corresponding to the button e When delete button is pushed activated objects including profile lines and measurement tools are deleted e The activated profile lines are red colored The other profile lines are blue e Only activated profile lines are displayed in the profile window e Multiple object selecti
80. the statistics of that How to collect data 1 Push Settings button and select the measurement items to gather 2 Do measurement 3 Push Collect button in Measurement result window Collect Result Data collector gathers only data chosen in settings If there is no chosen item in the setting Data collector collects nothing Data Collector Collect Result Row Block Length Measur Radius Measur Area Measure Statistics 2 0 18 0 03 0 00 0 13 0 10 This button delete all data in the last gathered block This button sets what measurement items will be collected Statistics This table shows the statistics of gathered data Data Collector Collect Result Statistics Length Measur Radius Measur Area Measure Statistics Min E 0 03 0 00 Max de Mean Std Dev sum Samples Blocks Note Data Collector window is a DockingPane so that it could be located anywhere When the window is overlapped to other DockingPane each DockingPane is distinguished by Tab 9 6 1 Data Collector Settings This function decides what measurement items to collect The items include not only the measurement values but also statistical values minimum maximum average standard deviation sum number of samples Data Collector Settings Measurements Length Measurements Value Radius Measurements Value Area Measurements Value Angle Count Start X Pos Start Y Pos End X Pos
81. to ROI region or to the whole image region Save Data This button saves the data as a csv file Save image This button saves the image of the histogram as a bmp file Note Intensity 03R 0 59G 0 11B Note Histogram is useful to control White Balance WB If the image of a white paper sample is correctly white balanced the histogram curves of R G B are almost same Note Since the Histogram window is DockingPane the window could be attached anywhere When the window is overlapped to other DockingPane each DockingPane is distinguished by Tab 5 Navigator Navigator shows the thumbnail image of the activated image and the view region displayed now as a red rectangle With the white rectangle in the bottom right corner of the red rectangle it is possible to zoom in or zoom out the image Also it is possible to change the view region by dragging the red rectangle Note Since the Navigator window is DockingPane the window could be attached anywhere When the window is overlapped to other DockingPane each DockingPane is distinguished by Tab 5 8 Tab Tab shows the names of opened images as tabs If a tab is selected the corresponding image is activated If mouse right button is clicked on a tab the save close menu would appear ang 86 65 X El Kimberly png 100 11 x El mosquito_1_f01 jpg 10 x El Panasis jpg E Blobs png 92 75 x El checke
82. urement data of measurement objects Label and value both shows labels and measurement values all Label only shows labels only Value only shows values only None does not show labels neither values Decimal places decides to what decimal places to show Note decimal places determined in measurement configuration 3 Management Service You can change crash error reporting set up and password Preference Management Service General Camera Crash error report Shortcut Layout Disable crash error report HUVIT Upright Microscope Measurement Change password Current password po Crash error report This box sets up whether you will automatically report the error to developers when Panasis ended abnormally before Change password This box changes the password for Environmental option and backup Initial password is 0000 3 8 Exit Alt F4 Exit quits Panasis If there are unsaved images Panasis asks whether you will save the images or not 4 Edit Menu m Redo Ctrl Y m Copy Ctrl C m Cut Ctrl X m Paste Ctrl V Delete Del m Select All Ctrl A m Select Mode F1 m Pan Mode F2 m ROI Mode F3 4 1 Undo Ctrl Z This function restores an activated image to the image before the last executed function was applied The functions which Undo could be applied to are the followings Addition Edition Delete of measuring objects and annotation objects ROI C
83. us indicator snap measurement and annotation e The snapped image has the calibration of that of Live Measurement window Also measurement objects annotations and scale are snapped too Live measurement 71 19 i s CR 1 0 03 5 N 9 5 Measure result Measured data are listed in Measure result table Measurements This table show all measurement data of the activated window If an item is selected in the table the corresponding measuring object is selected too Vice versa if a measuring object is selected in an image the corresponding data are selected too Measure Result Measurement item Y Pos Length Radius Diameter Area Start XP Rui 36 0 07 0 18 0 03 0 06 LN 2 Br 0 13 0 19 LN 3 E t 0 10 0 27 RT 4 E 0 22 This button collect all measurement data of the activated window to data collector Statistics This table shows statistics of the measurement data in the activated window Measure Result Measurements Statistics X Pos Y Pos Length Radius Diameter Area Start XP as Min 3 0 06 Max B 0 11 Mean d 0 08 Std Dev Oi 0 02 Sum k 0 34 Samples 1 4 00 Note Measurement result window is a DockingPane so that it could be located anywhere When the window is overlapped to other DockingPane each DockingPane is distinguished by Tab 9 6 Data Collector This function collects the measured data and shows the data and

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