QuickStep™2 384-Well PCR Purification Kit
Contents
1. A EdgeBio QuickStep 2 384 Well PCR Purification Kit Product Catalog Purifications QuickStep2 384 Well PCR 39231 768 Purification Kit 2 Plates QuickStep2 384 Well PCR 89815 3840 Purification Kit 10 Plates Description The QuickStep2 384 Well PCR Purification Kit delivers highly purified DNA that can be immediately used for further amplification cloning and sequencing reactions The kit consists of two purifying reagents an improved patented SOPE Solid phase Oligo Protein Elimination Resin that binds primers ssDNA enzymes and other proteins and the PERFORMA 384 Well Plate that eliminates up to 99 of salts buffers dNTPs and other small molecules Best results are obtained when using PCR reaction volumes of 5 15 ul Kit Components 39231 89815 SOPE Resin 3 5 ml 12 ml PN 4050173 PN 4050174 Performa 384 Well 2 plates 10 plates Plate 2x PN 4050183 10x PN 4050183 Equipment and Materials Required 1 Variable speed centrifuge benchtop or floor model capable of 850 x g 2 Rotor and microplate carriers for above 384 well flat bottom plate 4 384 well V bottom plate Storage Condition Store the SOPE Resin and the Performa 384 Well Plates at 4 C Do not freeze Quality Control Tested for DNA recovery primer protein and salt removal DNA recovered is tested to assure functionality in sequencing reactions Warning This product is intended for research use only It is not to be used fo2. r diagnostic purposes in humans or animals Recommended Protocol 1 2 10 11 Briefly vortex the SOPE Resin to mix Add 1 5 volume relative to the volume of the PCR reaction of SOPE Resin directly to the PCR reaction mixture as in the table below SOPE Volume Reaction Volume 1 ul 5 ul 2 ul 10 ul 3 ul 15 ul Mix well Let the suspension stand at room temperature while preparing the Performa 384 Well Plate Remove adhesive plate sealers from the top and bottom of the Performa 384 Well Plate Stack the Performa 384 Well Plate on top of the 384 well flat bottom receiver plate Place the assembly in a cushioned centrifuge plate carrier Centrifuge for 2 minutes at 850 x g e Note See Additional Notes for determination of RPM from RCF or visit our website at www edgebio com and click on Technical Support Transfer the SOPE PCR reaction mixture by slowly pipetting directly to the wells of the Performa 384 Well Plate Be sure fluid runs into the gel matrix Stack the Performa 384 Well Plate on top of the 384 well V bottom receiver plate Place the assembly in a cushioned centrifuge plate carrier Centrifuge for 5 minutes at 850 x g Retain eluates Page 1 of 2 v 2 Additional Notes Conversion of RCF to RPM Calculation An accurate determination of the centrifugation speed is very important The relative centrifugal force RCF specified in the protocol is converted to revolu
3. tions per minute RPM using the following formula 2 RCF sad aN 1000 The radius r is equal to the distance in millimeters between the axis of rotation and the bottom of the gel bed when the plate is placed in the plate carrier in the centrifuge bucket After measuring the radius for the specific centrifuge and accessories to be used the proper RPM setting is calculated as follows RPM 1000 nr 1 12r To achieve RCF 850 x g RPM 27 500 ft P Warning This product is intended for research use only Itis not to be used for diagnostic purposes in humans or animals Page 2 of 2
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