Model 975/2274 Syringe Pump Series Manual
Contents
1. ring syringe is preferrerd Binding of glass syringes may sometimes occur when the syringe plunger is extended to its maximum Satisfactory results can be obtained by using a larger capacity syringe at less than maximum volume A wide variety of plastic glass and stainless steel syringes as well as stopcocks and Luer adapters are available from Harvard Apparatus Consult our Harvard Bioscience Full Line Catalog Flow Rate 975 BG EP pqym z7 975 amp 2274 Syringe Pump User s Manual Harvard Apparatus Model The flow rate table on the pump shows the flow rate per syringe expressed in cc minute The gear box provides 30 positions each numbered To shift gears push the flow selector C refer to figure page 4 tow ard the syringe plate and slide itto the desired position See step 2 Until you become accustomed to handling the flow selector accuracy can be assured by checking the high and low flow selector positions notches by looking through the slot It is not necessary to turn off the pump when shifting from one flow rate to another Because of the involvement of many gears the pump may require one or two minutes of build up time for procedures carried out at lower speeds It is advisable to turn the pump on and allow it to run at the desired flow rate setting while syringes are being filled and placed in the syringe holder D A lead time of a couple of minutes may be nec essary when the gear position is2. attention is the drive 26 50 ml 104 screw This should be oiled one or two drops of light oil approximately twice a year Flow Rate 2274 Continued 12 PP TY AHA w Flow Rates l min for Harvard Classic Mechanical 2274 Syringe Pump z Gear Syringe Size Harvard Gas Tight Microliter Hamilton Series 1000 5 Position 10 ul 25 pl 50 pl 100 pl 250 ul 500 ul 1ml 2 5 ml ir 1 0 126 0 315 0 630 1 26 3 15 6 30 12 6 31 5 5 2 0 0900 0 225 0 450 0 900 2 25 4 50 9 00 22 5 3 0 0643 0 161 0 321 0 643 1 61 3 21 6 43 16 1 4 0 0459 0 115 0 229 0 459 1 15 2 29 4 59 115 ka 5 0 0328 0 0820 0 164 0 328 0 820 1 64 3 28 8 20 0 6 0 0234 0 0586 0 117 0 234 0 586 1 17 2 34 5 86 C 7 0 0167 0 0418 0 0835 0 167 0 418 0 835 1 67 4 18 Joong 0 0299 foosa7 Jono 0299 0597 119 299 n 9 0 00854 0 0213 0 0426 0 0854 0 213 0 426 0 854 2 13 x 10 0 00610 0 0152 0 0304 0 0610 0 152 0 304 0 610 1 52 a 11 0 00436 0 0109 0 0217 0 0436 0 109 0 217 0 436 1 09 12 0 00311 0 00778 0 0155 0 0311 0 0778 0 155 0 311 0 778 n 13 0 00222 0 00556 0 0111 0 0222 0 0556 0 111 0 222 0 556 ph 14 0 00159 0 00397 0 00792 0 0159 0 0397 0 0792 0 159 0 397 F 15 0 00114 0 00283 0 00566 0 0111 0 0283 0 0566 0 114 0 283 ps 16 0 000811 0 00202 0 00404 0 00811 0 0202 0 0404 0 0811 0 202 5 17 0 000580 0 00145 0 00289 0 00580 0 0145 0 0289 0 0580 0 145 4 18 0 000414 0 00103 0 00206 0 004
3. between 15 and 30 At higher pump ing rates below 15 pumping action will be virtually instantaneous Flow Rates for Non Standard Syringes To find the correct Flow Selector setting for a desired flow rate when using a syringe other than one of the four standard B D size calibrated on the side of the Compact Syringe Pump carefully measure the diame ter of the syringe plunger and refer to the next two pages Harvard Classical Mechanical 975 Syringe Pump Nominal Flow Rate ml min Syringe Size Position 5 ml 1 8 04 23 6 2 5 74 16 9 3 4 10 12 1 4 2 93 8 61 5 2 09 6 15 6 1 49 4 39 7 1 07 3 14 8 0 763 2 24 9 0 545 1 60 10 0 389 1 14 11 0 278 0 816 12 0 198 0 583 13 0 142 0 416 14 0 101 0 297 15 0 0724 0 212 16 0 0517 0 152 17 0 0369 0 108 18 0 0264 0 0774 19 0 0188 0 0553 20 0 0135 0 0395 21 0 00961 0 0282 22 0 00687 0 0201 23 0 00490 0 0144 24 0 00350 0 0103 25 0 00250 0 00734 26 0 00179 0 00525 27 0 00128 0 00375 28 0 000912 0 00268 29 0 000651 0 00191 30 0 000465 0 00136 46 3 33 1 23 6 16 9 12 1 8 64 6 17 4 41 3 15 2 25 1 61 1 15 0 821 0 586 0 419 0 299 0 214 0 153 0 109 0 0779 0 0556 0 0397 0 0284 0 0203 0 0145 0 0104 0 00743 0 00531 0 00379 0 00271 11 4 55 3 39 0 28 0 20 2 14 4 10 3 1 34 5 25 3 75 2 68 1 91 1 36 0 975 0 696 0 497 0 355 0 254 0 181 0 129 0 0925 0 0661 0 0472 0 0337 0 0241 0 0172
4. 0 0123 0 00877 0 00626 0 00447 Flow Rate Non Standard Syringes 9 PPR ee w Flow Selector Position 975 Diameter of Plunger Flow Rates cc min o 1 8 3 16 1 4 5 16 3 8 7 16 1 2 9 16 5 8 z 1 59 1 35 2 39 3 75 5 41 735 9 57 12 1 15 0 2 421 964 1 71 2 68 3 86 5 25 6 83 8 64 10 7 Y 3 301 688 1 22 191 2 78 3 75 4 88 6 17 7 64 4 215 491 871 1 36 1 97 2 68 3 49 4 41 5 46 3 5 154 351 622 971 141 191 2 49 3 15 3 90 a dj 6 110 251 444 693 101 1 36 1 78 2 25 2 79 7 0786 179 317 495 121 971 1 27 1 61 1 99 Ta 8 0561 128 226 354 515 693 907 115 1 42 A 9 0401 0914 161 253 368 495 648 114 1 01 10 0286 0653 115 181 263 354 463 510 121 N a 11 0204 0466 0821 129 188 253 331 364 515 12 0146 0333 0586 0921 134 181 236 260 368 jk 13 0104 0238 0419 0658 0957 129 169 186 263 14 00743 0170 0299 f 0470 0683 0927 121 133 188 15 00531 0121 0214 1 0336 0488 0658 0864 0950 134 16 00379 00864 0153 f 0240 0349 f 0470 0617 0678 0957 17 00271 00617 0109 0171 0249 1 0336 0441 0484 0683 18 00195 00441 00778 0122 1 0178 f 0240 0315 0346 0488 19 00139 00315 00556 00871 0127 1 0171 7 0225 0247 0349 20 000993 00225 f 00397 00622 00907 0122 0161 0176 0249 21 000709 00161 00284 00444 00648 00871 0115 0126 0178 22 000
5. 0196 00221 00249 00279 00315 00346 Maintenance 1 975 amp 2274 Syringe Pump User s Manual Harvard Apparatus Model Should a significant amount of fluid be spilled into the mechanism return pump to fac tory for servicing The Compact Syringe Pump has its own electric fuse located near the entrance of the power cord With the power ON if the pump motor does not run and the pilot light does not light examine the fuse If blown replace it If it blows again return the unit to Harvard Apparatus for servicing Power Consumption 60 Watts max Fuse 1 4 Amp Slo Blo all models Flow Rate 2274 2274 Microliter Rates Harvard Classical Mechanical To find the correct Flow Selector setting for a PUSIT desired flow rate when using a syringe between 500hl to 50 ml See Example to right Nominal Flow Rate ml min Gear 2274 Backlash HR F A The Model 2274 pump contains many gears Position Syringe Size Flow Rate which reduce the speed of the drivetrain to 13 500 ul 111 extremely slow speeds There is a finite time for 15 1 0 ml 114 gears to catch up backlash For best opera 8 25 mi 03 tion particularly at low speeds the pump 1 5m 1 should be turned on and the proper gear posi 20 5 ml 105 tion selected well before the onset of pumping 2 10 ml 108 30 60 minutes is not unusual at slow rates Backlash increases with the higher gear position 24 20 ml 0993 numbers 25 30 ml 0949 The only area requiring
6. 14 0 0103 0 0206 0 0414 0 103 19 0 000300 0 000738 0 00147 0 00300 0 00738 0 0147 0 0300 0 0738 a 20 0 000211 0 000527 0 00105 0 00211 0 00527 0 0105 0 0211 0 0527 A 21 0 000151 0 000376 0 000752 0 00151 0 00376 0 00752 0 0151 0 0376 lt 22 0 000108 0 000269 0 000537 0 00108 0 00269 0 00537 0 0108 0 0269 23 0 0000769 0 000192 0 000384 0 000769 0 00192 0 00384 0 00769 0 0192 gt 24 0 0000550 0 000137 0 000274 0 000550 0 00137 0 00274 0 00550 0 0137 8 25 0 0000393 0 0000930 0 000196 0 000393 0 000980 0 00176 0 00393 0 00980 26 0 0000281 0 0000700 0 000140 0 000281 0 000700 0 00140 0 00281 0 00700 27 0 0000200 0 0000500 0 0000998 0 000200 0 000500 0 000998 0 00200 0 00500 28 0 0000143 0 0000357 0 0000713 0 000143 0 000357 0 000713 0 00143 0 00357 29 0 0000102 0 0000255 0 0000509 0 000102 0 000255 0 000509 0 00102 0 0025 o o 0 00000073 0 0000182 0 0000364 0 000073 0 000182 0 000364 0 00073 0 000182
7. 2 in Weight 5 4 hg 12 lb Pump 2274 Type Mechanical multiple syringe infusion only Motor 30 speed 100 times slower than pump 975 Syringe Size Glass and Plastic Holder for up to 2 Syringes 10 ml to 100 ml Adjustable Holder for up to 3 Syringes 20 ml Only Holder for up to 4 Syringes 5 ml Only Dimensions H x W x D Weight 335 x 171 x 89 mm 14 x 6 3 4 x 3 1 2 in 5 4 hg 12 lb Catalog No Description 55 1689 975 115 VAC 60 Hz 55 1697 975A 230 VAC 50 Hz 55 1705 975B 115 VAC 50 Hz 55 3206 2274 115 VAC 60 Hz 55 3214 2274A 230 VAC 50 Hz Pump Layout a7 rr 975 amp 2274 Syringe Pump User s Manual Harvard Apparatus Model Thumb Syringe ON OFF Switch Release Pusher Syringe Clamp ilot Li Pilot Light mn Syringe Holder i Flow Selector Getting Acguainted With Your Compact Syringe Pump Before using the Compact Syringe Pump we suggest you run through the following familiarization procedure Step 1 Set the Compact Syringe Pump on a table with the Flow Chart facing you Plug the cord into an electrical outlet and snap the ON OFF switch to ON Listen to the sound of the pump running Note that the Pilot Light is on Caution This pump must always be use with a 3 wire grounded electrical outlet If your location has only 2 wire receptacles consult you electrician for proper grounding procedures Step 2 Push the Flow Selector away from you and slide it lat
8. 3 2 89 11 622 743 871 101 117 133 150 167 168 2 06 12 444 531 1 622 721 836 950 107 119 134 1 47 13 317 379 444 515 597 678 764 850 957 1 05 14 226 271 317 368 426 484 546 607 683 150 15 161 194 226 263 304 346 390 434 488 536 975 amp 2274 Syringe Pump User s Manual 16 115 1 139 161 188 217 f 247 279 310 349 383 17 0821 0993 115 134 155 176 199 221 249 214 18 0586 0709 0821 0957 111 126 142 158 178 196 19 0419 0506 0586 0683 0793 0900 101 113 127 140 20 0299 0361 1 0419 0488 0566 0643 0721 0807 0907 100 21 0214 0258 0299 0349 0404 0459 0515 0576 0648 0714 22 0153 0184 0214 0249 0289 0328 0368 0411 0463 0510 23 0109 0131 1 0153 0178 0206 0234 0263 0294 0331 0364 24 00778 00935 0109 0127 0147 0167 0188 0210 0236 0260 25 00556 00668 00778 00907 0105 0119 0134 0150 0169 0186 Harvard Apparatus Model 26 00397 00477 00556 00648 00750 00850 00957 0107 0121 0133 27 00284 00341 00397 00463 00536 00607 00683 00764 00864 00950 28 00203 00244 00284 00331 00383 00434 00488 00546 00617 00678 29 00145 00174 00203 00236 00274 00310 00349 00390 00441 00484 30 00104 00124 f 00145 00169 0
9. 506 00115 f 00203 00317 00463 00622 00821 00900 0127 23 000361 000821 00145 00226 00331 00444 00586 00643 00907 24 000258 000586 00104 00161 00236 00317 00419 00459 00648 25 000184 000419 000742 00115 00169 00226 00299 00328 00463 Harvard Apparatus Model 26 000131 000299 000530 000821 00121 00161 00214 00234 00331 27 0000936 000214 000379 000586 000864 00115 00153 00167 00236 28 0000668 000153 000271 000419 000617 000821 00109 00119 00169 29 0000477 000109 000194 000299 000441 000586 000778 000850 00121 30 0000341 0000778 000139 000214 000315 000419 000556 000607 000864 Flow Rate Non Standard Syringes 10 CA Flow Selector Position 975 Diameter of Plunger Flow Rates cc min 11 16 3 4 13 16 7 8 15 16 f 1 11 16 1 1 8 1 13 16 1 1 4 1 18 1 216 253 294 33 7 383 43 2 484 54 0 59 7 2 129 154 7181 7210 241 274 309 346 38 6 42 6 3 921 11 0 129 150 172 196 22 1 247 27 6 30 4 4 658 786 921 107 123 140 158 176 19 7 21 7 5 470 561 658 764 878 100 1 3 126 141 15 5 6 336 401 74 70 546 627 714 807 900 10 1 11 1 7 239 286 73 36 390 448 510 576 643 7 21 1 93 8 171 204 240 279 320 364 411 459 5 15 5 66 9 122 146 171 199 229 260 294 328 3 68 4 04 10 871 104 122 142 164 186 210 234 26
10. Model 975 2274 Syringe Pump Series Users Manual Model 975 Syringe Pump 55 1689 Model 2274 Syringe Pump 55 3206 Table of Contents 1 i T aaa nG GB GT Warranty and Repair Information 0 2 Specifications sisscissc ss akn s ems asa esmkumus mkan susaguniaaaa 3 Pump Layout ee 4 5 DESCHPUOM AA 6 Syringe ClaMPS una nananana aaa 7 SI AA AA 7 Flow Rate 975 saaan 8 Flow Rate Non Standard Syringes 9 10 Maintenance ee 11 Flow Rate 2274 ss saska 11 Harvard Apparatus Model 975 amp 2274 Syringe Pump User s Manual Warranty and Repair Information IRE 975 amp 2274 Syringe Pump User s Manual Harvard Apparatus Model Serial Numbers All inquires concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the chassis Calibrations All electrical apparatus is calibrated at rated voltage and frequency While the flow will stay calibrated the peak will vary Warranty Harvard Apparatus warranties this instrument for a period of one year from date of purchase At its option Harvard Apparatus will repair or replace the unit if it is found to be defective as to workmanship or material This warranty does not extend to damage resulting from misuse neglect or abuse nor mal wear and tear or accident This warranty extends only to the original customer purchaser IN NO EVENT SHALL HARVARD APPARATUS BE LIABLE FOR INCIDENTAL OR CONSEQUE
11. NTIAL DAMAGES Some states do not allow exclusion or limitation of incidental or conse quential damages so the above limitation or exclusion may not apply to you THERE ARE NO IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR USE OR OF ANY OTHER NATURE Some states do not allow this limitation on an implied warran ty so the above limitation may not apply to you If a defect arises within the one year warranty period promptly contact Harvard Apparatus Inc 84 October Hill Road Building 7 Holliston Massachusetts 01746 1371 using our toll free number 1 800 272 2775 Goods will not be accepted for return unless an RMA returned materials authorization number has been issued by our cus tomer service department The customer is responsible for shipping charges Please allow a reasonable period of time for completion of repairs replacement and return If the unit is replaced the replacement unit is covered only for the remainder of the original warranty period dating from the purchase of the original device This warranty gives you specific rights and you may also have other rights which vary from state to state Repair Facilities and Parts Harvard Apparatus stocks replacement and repair parts When ordering please describe parts as completely as possible preferably using our part numbers If practi cal enclose a sample or draw ing We offer a complete reconditioning service CAUTION This pump is not registered wit
12. erally back and forth Release the Flow Selector and feel it drop into one of the 30 numbered slots The slot you choose determines the speed that the Syringe Pusher advances the syringe plunger Note that when you choose any of the odd numbered slots such as 1 the fastest speed the How Selector drops only about half way down When you choose any of the even numbered slots such as 2 the Flow Selector drops all the way down The position of each number on the scale shows you whether that slot position is halfway down or all the way down Lever Up Lever Down Pump Layout C 7 7laaar rr 975 amp 2274 Syringe Pump User s Manual Harvard Apparatus Model Step 3 Grasp the Syringe Plunger push down on the Thumb Release and slide the Syringe Pusher back and forth Note that when you let go of the Thumb Release the Syringe Pusher stays in place Step 4 Turn the pump on Position the Syringe Pusher to the right as far as it will go This is its position after the pump has emptied a syringe Position the Flow Selector in slot number 1 the fastest pumping speed Listen to the pump Immediately you will hear a rapid clicking This is perfectly normal and occurs whenever the Syringe Pusher movement is obstructed At slower pumping speeds the clicks occur less fre quently At the slowest speed Flow Selector position number 30 a single click occurs only once every hour or so Step 5 The Compac
13. h the FDA and is not for clinical use on human patients A CAUTION Not for clinical use on human patients Specifications 3 975 amp 2274 Syringe Pump User s Manual Harvard Apparatus Model This is the pump that made Harvard s reputation for reliability and precision The DC motor and gear box provide low flow rates and a complete absence of pulsation Its synchronous motor is regulated by the supply line frequency giving the ultimate accu racy 0 01 Its thirty speed mechanical gear box is an engineering marvel that will give years of service The pump has speed accuracy of 0 01 and a 30 speed mechanical gear box with positive locking action The ratio between adjacent settings has been held to only 1 4 to 1 The range over the 30 gear settings is 17 000 to 1 A gear overload clutch stops pumping at a back pressure of about 25 p s i This Pump s syringe holder can hold either one or two plastic or glass syringes of any size from 2 ml to 100 ml Two additional syringe holders are supplied and can be eas ily installed The first will hold up to three 20 ml syringes and the second up to four 5 ml syringes Specifications Pump 975 Type Mechanical multiple syringe infusion only Motor 30 speed Syringe Size Glass and Plastic Holder for up to 2 Syringes 2 ml to 100 ml Adjustable Holder for up to 3 Syringes 20 ml Only Holder for up to 4 Syringes 5 ml Only Dimensions H x W x D 335 x 171 x 89 mm 14 x 6 3 4 x 3 1
14. sion rates are possible The maximum rate using two 100 ml syringes is 154 8 ml per minute while the slow est rate using one 1 cc tuberculin syringe is 000077 cc per minute Syringe Clamps J 975 amp 2274 Syringe Pump User s Manual Harvard Apparatus Model Standard syringes are held in a universal syringe holder plate as explained in Step 5 The syringe pusher B can then be adjusted against the extended plunger of the syringe by pushing down on the thumb release lever G and sliding the syringe push er When the syringes have emptied the built in overload clutch will begin to click The pump will not automatically turn itself off The clutch mechanism will disengage and make a clicking sound with each revolution of the drive screw Binding in the syringes or clogging of the media will also cause the clutch to disengage pumping action For convenience in carrying out repetitive infusion procedures it is possible to refill the syringes without their removal from the pump by use of a three way stopcock F connected to the media reservoir Syringe The pump has been calibrated on the basis of Becton Dickinson multifit glass syringes although it will handle other types Plastic disposable syringes are excellent for procedures requiring minimum leakage Check actual delivery rates when using any other brand or type of syringe In general it has been found that when the media is viscous highly volatile or quick drying a plastic O
15. t Syringe Pump has a place for two syringes The picture below shows how a syringe fits in the two vee notches and how it is held in place by a Syringe Clamp The syringe barrel flange should be snug against the Syringe Holder as illus trated so that the barrel cannot move forward during pumping action Lift one of the syringe clamps and feel the spring pressure Turn it and observe how easily you can position it so that it holds the syringe in place 6 975 amp 2274 Syringe Pump User s Manual Harvard Apparatus Model Description The Compact Syringe Pump is designed for infusion emptying syringes only using glass disposable plastic stainless steel or glass O Ring Robb syringes from 100 to 1 cc ON OFF Switch E Drive Screw A N Syringe Pusher B Syringe J Stopcock F goes Thumb here Release G Flow Selector C Syringe Holder D The pump consists of a synchronous motor of 100 R PM a 30 step parallel shaft gear box clutch drive screw and syringe holder As illustrated the syringe J is drive by a drive screw A which moves a spring loaded half nut connected to the pusher plate B The 30 position gear box permits the instantaneous selection of any speed of over 17 000 to 1 range The ratio between adjacent rates is 1 4 to 1 The close coupled gear box permits exact changes in flow rate all at synchronous speeds Between the 30 speeds and the use of one or two syringes a total of 2400 infu
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