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1. Version1 0 rev 18 Feb 2015 18 Rotr ANDOR company APPENDIX A MECHANICAL DRAWINGS Dimensions in mm inches 135 7 5 34 189 4 7 46 ee Version1 0 rev 18 Feb 2015 19 ANDOR company APPENDIX B COMMANDS GET FILTER WHEEL STATUS Bee BRR Response When requesting the wheel status the device responds with the received command CC hexadecimal immediately and requests configuration information from a daisy chained wheel The status information of the whole chain is returned after a 1 4s timeout Byte 1 0xCC Bytes 0 10 pee n Byte 0 0xCC VVheel position VVheel speed 0000 0 Position 0 000 0 Speed 0 0001 1 Position 1 001 1 Speed 1 0010 2 Position 2 010 2 Speed 2 0011 3 Position 3 011 3 Speed 3 0100 4 Position 4 100 4 Speed 4 0101 5 Position 5 101 5 Speed 5 0110 6 Position 6 110 6 Speed 6 0111 7 Position 7 111 7 Speed 7 1000 8 Position 8 1001 9 Position 9 Version1 0 rev 18 Feb 2015 20 ANDOR company Byte 2 WBST Wheel B Speed and Position H BINI VVheel position Bit Description Byte 4 WCST Wheel C Speed and Position Description c Version1 0 rev 18 Feb 2015 Wheel speed 000 0 Speed 0 001 12. Micro Manager 3 3 ROTR FILTER WHEEL FUNCTIONS Rotr filter wheel offers exceptionally high switching speeds and is mounted at an angle between the CSU and camera for optimum performance with no back reflections The filter wheel has the following functions Houses powers and controls up to 10 filters of 25mm diameter Is controlled by PC based software via a RS 232 cable supplied Controls the filter position and speed of switching Allows the control of up to 3 filter wheels by daisy chaining the devices te Version1 0 rev 18 Feb 2015 11 ANDOR company SECTION 4 INSTALLATION 4 1 INSTALLING THE ROTR FILTER WHEEL WARNING This product is supplied as part of a system and must be installed by an Andor Installation Technician or a trained Andor Systems distributor The following is only provided to augment this 4 1 1 LOCATION AND MOUNTING e Temperature and humidity must meet the specifications shown in Section 7 2 Operating Conditions e Operational vibrations should be eliminated Ensure the stand is fully clamped to the optical table using 2 screws per clamp ring where possible e Power cabling and control cables should be routed to prevent accidents damage and accidental unplugging Installation into a Revolution System by an Andor Installation Technician or trained Andor Systems di
3. ANDOR an Oxford Instruments company Rotr Version 1 0 rev 18 Feb 2015 Rotr ANDOR Hardware Guide Andor Technology 2015 ANDOR company TABLE OF CONTENTS Sad 10 R TON o E ee ee n s SA 3 Safety and Warning Information ccccccccccesescccuecccuscccceesceuecscenecseeesseeuesseuuseseueceeeuessueueseeeueeseueseseueceseuessneneseeeueeeees 4 SECTION 1 Sounooveotedeowadeassvistedeewaceuates 5 II 11606 SUD DOU 5 w2 DEA 6 1 T m s n 6 SECTION 2 PRODUCT OVERVIEW baa aa daaa daaa aaa a D bd sad d pi 7 21 dord ST L a a o Ban n 7 2 Ptr hEe L b EE 8 SECTION 3 FEATURES AND FUNCTIONALITY OF THE ROTR ssssssssssssssssssssssssssssssssssesseessessseesessesseeaeeas 10 251 Aboutthe Revolution tema ala R EAEE 10 22 REU 0005 o il ee 11 3 3 Rotr Filter ay pa 11 SECTION 4 INSTALLATION a a Oe a aaa a papa R s 12 OA Installing 12 4 1 1 CAA Bey At IV OE WN steerer a l n 12 4 1 2 cami E EA 13 4 1 3 Power Connection and Protective 13 21 ee 13 4 2 1 P werand h Es b b n inn 13 10 117 1 liE 13 SECTION 5 USING THE ROTR FILTER VUF EEL L na aaa bada
4. cord is required ensure replacement is of same type and rating 11 While running an experiment try to keep room temperature as stable as possible 12 Performance of the system may be adversely affected by rapidly changing environmental conditions or operation outside of the operating conditions specified in Technical Specifications 13 This product is not designed to provide protection from ionising radiation Any customer using this product in such an application should provide their own protection 14 This is a Class A product In a domestic environment this product may cause electromagnetic interference in which case the user may be required to take adequate measures 15 This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a non residential environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case the user will be required to correct the interference at their own expense 16 Shielded I O cables must be used when operating this equipment 17 Pollution Degree 2 Normally only non cond
5. na yaaa bala anada n B 14 51 Emergency DISCOMMC 14 5 TUCLORBUrTU Ce aaa an ii 14 5 3 Power Up SC r ib baban 14 Dit POWEFDOWM 14 SECTION 6 15 ul Cleaning and Decontam na hu 15 Ce 2 711 b r si 15 63 Arnnual Electrical Cecen aaa 15 075 141 CAN s 15 SEL U L SEE Lo yr daya ada eee a 17 2 1 1701770100 s E 17 Version1 0 rev 18 Feb 2015 2 ANDOR company Operating Go a 6 0 9 eee au ne ee ee eee eer 17 5 IVE CAC A aaa abad 18 714 amrnspofte Storage Se Ci Ca 10R Dil 18 APPENDIX A MECHANICAL DRAWINGS 52255225 222 19 APPENDIX BCOMMANDS a an n 20 Get Filter ui n sils 20 G LET heecliconi2i r A al 23 BU WV CSI PO tiOf ste a a aaa 25 7000 100 o b 0 0 26 Bee r m i s n 26 mi 27 28 Terms and Conditions of Sale and Warranty 28 Waste E
6. ABLING Refer to Sections 2 2 and 7 1 4 3 INSTALLING THE SOFTWARE Refer to the Software Guide supplied with your software for details e Version1 0 rev 18 Feb 2015 13 ANDOR company SECTION 5 USING THE ROTR FILTER WHEEL WARNING If the equipment is used in a manner not specified by Andor the protection provided by the equipment may be impaired 5 1 EMERGENCY DISCONNECTION In case of emergency the disconnecting device is the mains lead This will either be the mains lead connected to the product or in the case of a cabinet based system the mains lead to the cabinet SWITCH OFF THE MAINS SOCKET AND REMOVE THE MAINS LEAD FROM THE PRODUCT 5 2 INSTRUCTIONS FOR USE The main operation of this product is as part of the wider Revolution XD system and this is covered in the applicable software User Guide and by the training given by your Andor Installation Technician or trained Andor Systems distributor 5 3 POWER UP SEQUENCE 1 Power up the filter wheel The motor should be audible as the filter wheel is turned to confirm the 0 position filter 2 Wait 1 minute to allow the Rotr device to stabilise 3 Power up the workstation 4 Wait for 5 minutes to allow the system to fully boot up and stabilise 5 Enable the control software Please refer to software user manual for operating protocols 6 Wait for a minimum of 5 minutes before commencing with the experiment 7 Whilst
7. AND FUNCTIONALITY OF THE ROTR 3 1 ABOUT THE REVOLUTION SYSTEM The Revolution XD and WD Systems provide a framework for Andor s laser spinning disk live cell confocal microscopy solutions which combine our award winning iXon Electron Multiplying CCD EMCCD camera with the renowned Yokogawa CSU X1 and CSU W1 The typical Revolution XD or WD System shown below encompasses a range of complementary components both hardware and software that fit seamlessly together creating a complete confocal microscopy solution A flexible component focus also allows us to provide key pieces of hardware as stand alone N x 0 o 2 oc O gt o 5 8 O Zo o id D co 2 2 2 Q x 8 2B 2 oN 5 N oo D oh s O O gt A O lt oO i 0 D EL 5 D A OQ D 2 YO amp m be D 2 o o Oo lt 5 gt o o 9 a WER 2 x 5 Yi 1 ya it ip 7 Z o o 5 O 0 Figure 6 Overview of Rotr integrated into a typical system Version1 0 rev 18 Feb 2015 10 ANDOR company 3 2 REQUIRED SOFTVVARE To control the Rotr you will need one of the following software packages e Q available from Andor e MetaMorph
8. HL1 WHLO 17 SHFUN1 SHFUNO Wheel selection Byte 1 2 open Description 10 2 wheel A 3 open on ext trigger 11 3 wheel B 4 close Response Byte 1 command byte as received returned immediately Byte 2 OxOD after task completion RESET Byte 1 0xFB Response 0x0D Version1 0 rev 18 Feb 2015 26 ANDOR company EXAMPLES Hexadecimal Functionality 00000000 Wheel A moves to position at maximum speed 10000000 us VVheel B moves to position 0 at maximum speed 11111100 Wheel C moves to position 0 at maximum speed 00000000 00110110 Wheel A moves to position 6 at speed 3 10101010 a Shutter A opens 10101100 AC 172 Shutter A closes 10111010 Shutter B opens ti Version1 0 rev 18 Feb 2015 27 ANDOR company APPENDIX C OTHER INFORMATION TERMS AND CONDITIONS OF SALE AND WARRANTY INFORMATION The terms and conditions of sale including warranty conditions will have been made available during the ordering process The current version may be viewed at http VVVVVV andor com pdfs literature Andor Standard VVarraniy pdf VVASTE ELECTRONIC AND ELECTRICAL EQUIPMENT REGULATIONS 2006 VVEEE The company s statement on the disposal of WEEE can be found in the Terms and Conditions Version1 0 rev 18 Feb 2015 23
9. Speed 1 010 2 Speed 2 011 3 Speed 3 100 4 Speed 4 101 5 Speed 5 110 6 Speed 6 111 7 Speed 7 VVheel speed 000 0 Speed 0 001 1 Speed 1 010 2 Speed 2 011 3 Speed 3 100 4 Speed 4 101 5 Speed 5 110 6 Speed 6 111 7 Speed 7 0000 0 Position 0 0001 1 Position 1 0010 2 Position 2 0011 3 Position 3 0100 4 Position 4 0101 5 Position 5 0110 6 Position 6 0111 7 Position 7 1000 8 Position 8 1001 9 Position 9 0000 0 Position 0 0001 1 Position 1 0010 2 Position 2 0011 3 Position 3 0100 4 Position 4 0101 5 Position 5 0110 6 Position 6 0111 7 Position 7 1000 8 Position 8 1001 9 Position 9 21 ANDOR company Byte 5 SAST Shutter A 010 1 2 Open on ext trigger 100 3 Closed 010 1 Open 2 Open on ext trigger 100 3 Closed Shutter Mode 011 3 Not Connected 100 4 Normal operation Shutter Mode 011 3 Not Connected 100 4 Normal operation Version1 0 rev 18 Feb 2015 22 ANDOR company GET FILTER WHEEL CONFIGURATION Byte 1 0xFD Response When requesting the wheel configuration the device responds with the received command FD hexadecimal immediately and requests configuration information from a daisy chained wheel The configuration information of the whole chain is returned after a 1 4s timeo
10. ged 6 4 INSTALLING FILTERS 1 Access to the filters is through the access port as indicated Using a flat head screwdriver or similar tool unscrew the cover anti clockwise 3 Carefully insert the filter noting the orientation of the filter to suit the image direction normally arrow toward camera side ed Version1 0 rev 18 Feb 2015 15 ANDOR company 4 Re install the retaining ring using a Thorlabs SPVV602 filter tool Be careful not to over tighten once the ring is clamping the filter into the installed position 5 Ensure the filter is full 6 Re install the access panel using a flat head screwdriver or similar tool and screwing clockwise ed Version1 0 rev 18 Feb 2015 16 ANDOR company SECTION 7 SPECIFICATIONS 7 1 POWER SUPPLY This product must be powered via the rear 3 pin IEC C14 connector from an a c mains power supply with the following ratings Voltage Range 24V DC Frequency Range 50 60 Hz Power Rating max 120W Note that this is a technical term to say that the product will expect to be plugged into a typical mains socket and experience the typical transient voltages that appear there 7 2 OPERATING CONDITIONS Location Indoor use Operating Temperature 27955 Storage Temperature 0 50 C Operating Relative Humidity 70 non condensing Note that this is a
11. heel with filters Figure 1 View of the Rotr showing the main hardware features MOTOR The enclosed motor rotates the filters between the required filter positions FILTER WHEEL The filter wheel contains up to 10 filters STAND The stand enables the Rotr to be set to the correct optical height and secured to the optical table te I 1 Version1 0 rev 18 Feb 2015 7 ANDOR company 2 2 FILTER WHEEL INTERFACE The filter wheel interface panel is located on the rear panel of the Rotr Figure 2 Rear view of the Rotr showing the location of the connections A description of these connections is outlined below Figure 3 Rotr connections panel Pwr POWER For connection to the 24 V DC power supply unit AUX I O For connection to an approved suitable shutter using pins 4 and 7 Figure 4 AUX I O pinouts OUT RS 232 connection for connection to additional filter wheels By using the RS 232 connection you can control up to three high speed filter wheels by daisy chaining the devices as shown below e000 8 Version1 0 rev 18 Feb 2015 ANDOR company I Ir PC Rotr A Rotr B Rotr C Figure 5 Outline view showing connections for up to 3 Filter wheels IN RS 232 connection for connection to the control PC cee eee OO Version1 0 rev 18 Feb 2015 9 ANDOR company SECTION 3 FEATURES
12. lectronic and Electrical Equipment Regulations 2006 WEEE 28 REVISION HISTORY C 21 Jan 2013 Initial Release Presentation updated to current Formatting enhanced throughout Version1 0 rev 18 Feb 2015 3 ANDOR company SAFETY AND VVARNING NFORMATION A WARNING PLEASE READ THIS SAFETY INFORMATION BEFORE USING THIS PRODUCT 1 If the equipment is used in a manner not specified by Andor the protection provided by the equipment may be impaired 2 Before using the system please follow and adhere to all warnings safety manual handling and operating instructions located either on the product supplied documentation or in this guide Keep this guide in a safe place for future reference There are no user serviceable parts inside the product and the enclosure must not be opened Only authorised service personnel may service this equipment This equipment has not been designed and manufactured for the medical diagnosis of patients Only the correctly specified mains supply should be used Replacement fuse must be of same type and rating Only the AC DC external power supply provided with the product should be used Only the power supply cord provided with the product should be used Should this not be correct for your geographical area contact your local representative 10 Make sure the power supply cord is located so that it will not be subject to damage If replacement of the detachable power supply
13. ration and maintenance of the Rotr filter wheel This product forms part of the Revolution XD system and therefore this manual should be read in conjunction with all relevant manuals supplied with your system Please not that some of the equipment described in this manual may not be included in the set you have purchased 1 1 HELP AND TECHNICAL SUPPORT If you have any questions regarding the use of this equipment please contact the representative from whom your system was purchased or Europe Andor Technology 7 Millennium Way Springvale Business Park Belfast BT12 7AL Northern Ireland Tel 44 0 28 9023 7126 Fax 44 0 28 9031 0792 Asia Pacific Andor Technology Japan 4F NE Sarugakucho Building 2 7 6 Sarugaku Cho Chiyoda Ku Tokyo 101 0064 Japan Tel 81 3 3518 6488 Fax 81 3 3518 6489 o o Version1 0 rev 18 Feb 2015 USA Andor Technology 425 Sullivan Avenue Suite 3 South Windsor CT 06074 USA Tel 860 290 9211 Fax 860 290 9566 China Andor Technology Room 1213 Building B Luo Ke Time Square No 103 Huizhongli Chaoyang District Beijing 100101 P R China Tel 86 0 10 5129 4977 Fax 86 0 10 6445 5401 ANDOR company 1 2 DISCLAIMER Andor Technology assumes no liability for the use of the information contained in this document or the software which it describes The user assumes all risks There is no warranty of fitness for a particular purpose either expre
14. running an experiment try to keep room temperature as stable as possible 8 If the system is not required for a while it should be powered down 5 4 POWER DOWN SEQUENCE 1 Close down the controlling software please refer to software user manual for protocols 2 Wait for software tab to disappear from workstation start bar 3 Power down the workstation 4 Power down the Rotr filter wheel o o o Version1 0 rev 18 Feb 2015 14 ANDOR company SECTION 6 MAINTENANCE PLEASE NOTE the following f the equipment is used in a manner not specified by Andor the protection provided by the equipment may be impaired e Incase of emergency the disconnecting device is the mains lead This will either be the mains lead connected to the product or in the case of a cabinet based system the mains lead to the cabinet See Section 5 1 Emergency Disconnection 6 1 CLEANING AND DECONTAMINATION To clean the product only use a damp lint free cloth Do not wet the connectors Do not use solvents cleaning agents or aerosols 6 2 REGULAR CHECKS The state of the product should be checked regularly especially the integrity of the enclosure and the mains cable e Do not use equipment that is damaged 6 3 ANNUAL ELECTRICAL CHECKS t is advisable to check the integrity of the insulation and protective earth of the product on an annual basis e g U K PAT testing e Do not use equipment that is dama
15. ss or implied The information contained in this document is assumed to be correct but in no event shall Andor Technology be held responsible for typographical errors or changes in the software not reflected in this document The specifications contained in this document are subject to change without notice Any changes or modifications not expressly approved in this manual could void your authority to operate this equipment If you require further information about this unit you may contact your local representative The latest contact details for your local representative may be found at our website www andor com 1 3 TRADEMARKS Andor and the Andor logo are trademarks of Andor Technology Andor Technology is an Oxford Instruments company All other marks are property of their owners All other trademarks mentioned in this document are the property of their respective owners and are used herein solely for informational purposes only Changes are periodically made to the product and these will be incorporated into new editions of the manual New releases of the manual are available through MyAndor http my andor com login aspx eo Version1 0 rev 18 Feb 2015 6 ANDOR company SECTION 2 PRODUCT OVERVIEW 2 1 HARDVVARE DESCRIPTION An outline of the basic components of the Rotr are outlined below Once installed the Rotr is controlled through the software RoOtr ANDOR Motor Filter W
16. stributor will include the following steps 1 Attach the Rotr to the CSU using 2 off M4 grub screws Version1 0 rev 18 Feb 2015 12 ANDOR company 4 Attach adapters to the Rotr housing as per Andor Assembly drawing ENI 00766 For adapter part numbers refer to the sales order BOM da SS 5 Install the power cable and data cable per the connector IDs on the back of the filter wheel Section 2 2 4 1 2 ASSEMBLY This product itself requires no assembly Cables connections are covered in Section N N 4 1 3 POWER CONNECTION AND PROTECTIVE EARTHING See Section 5 1 Emergency Disconnection Before connection check that the mains power socket used can provide the power as specified Rotr Section 7 1 Power Supply Always switch off power before connecting disconnecting cables from the product A mains power cable is provided with this product but ensure that it satisfies local regulations for safety Mains power is connected via the 3 pin IEC C14 connector on the rear panel of the product e An integral part of protection against electric shock in the case of a fault is the protective earth provided via the earth conductors in the mains cable It is therefore vital that the earth system of the building and in particular the socket is constructed properly to provide suitable protection when needed e Do not pull cables by the sheath Use the connector body 4 2 INSTALLING CABLES 4 2 1 POWER AND SIGNAL C
17. technical term to say that normally only non conductive pollution occurs occasionally however a temporary conductivity caused by condensation must be expected e This product has not been designed to operate correctly in direct sunlight so it should be avoided The term indoor use also implies that it is not designed to expect water spills e Whilst running an experiment try to keep room temperature as stable as possible ee Version1 0 rev 18 Feb 2015 17 ANDOR company 7 3 MECHANICAL SPECIFICATIONS VVidth 136 mm Excluding connected cables at rear t Protection against fingers or other object not greater than 80 mm in length and 12 mm in diameter when installed into a Revolution system Not protected from harmful entry of various forms of moisture e g dripping spraying submersion etc 7 4 TRANSPORT amp STORAGE SPECIFICATIONS e The packaging used for the original delivery should be retained for further use This product must be shipped in accordance with the international standard ETS 300119 Class 2 1 System functional performance shall be maintained following exposure to a Storage Transportation temperature range of 0 C to 50 C The packaging must be protected from excesses of weather Relative humidity must not exceed 90 non condensing Shipped Dimension 520 x 370 x 220 mm 20 5 x 14 5 x 8 5 inches Approximate Shipped eo
18. uctive Pollution occurs Occasionally however a temporary conductivity caused by condensation must be expected 18 Your product is a precision scientific instrument containing fragile components Always handle it with care 19 Do not wet or spill liquids on the product and do not store or place liquids on the product 20 If spillage occurs on the product switch off power immediately and wipe off with a dry lint free cloth 21 If any ingress of liquids has occurred or is suspected unplug the mains cables do not use and contact Customer Support 22 Do not expose the product to extreme hot or cold temperatures 23 Do not expose the product to open flames 24 Do not allow objects to fall on the product 25 Refer to Section 6 1 for Cleaning information 7 go p g o o i Version1 0 rev 18 Feb 2015 4 SECTION 1 INTRODUCTION ANDOR company Thank you for purchasing an Andor Rotr 10 position filter wheel You are now in possession of a high speed filter wheel unit designed for the most challenging microscopy applications This manual contains useful information and advice to ensure you get the optimum performance from your system If you have any questions regarding your Roir please feel free to contact Andor directly or via your local representative or supplier The Rotr is designed to be used in research laboratories and other controlled scientific environments This manual is aimed at covering the ope
19. ut Bytes 0 4 WHLACFG Wheel A configuration NC Not Connected ER Error 25 zomm 32 32mm Bytes 10 14 VVheel B configuration NC Not Connected ER Error 25 25mm 32 32mm Version1 0 rev 18 Feb 2015 23 ANDOR company Bytes 15 19 Wheel C configuration NC Not Connected ER Error 25 25mm 32 382mm Bytes 20 24 24 Shutter A Type VS Vincent Shutter Bytes 25 29 790 s Shutter B Type S Vincent Shutter Bytes 30 ee Version1 0 rev 18 Feb 2015 24 ANDOR company SET WHEEL POSITION Byte 1 Byte 2 Name SPD2 SPD1 SPDO POS3 POS2 POS1 POSO Description Wheel position Wheel speed 0000 0 Position 0 000 0 Speed 0 0001 1 Position 1 Wheel 001 1 Speed 1 0010 2 Position 2 selection 5 2 Speed 2 0011 3 Position 3 J 011 3 Speed 3 0100 4 Position 4 1 Wheel 100 4 Speed 4 0101 5 Position 5 B 101 5 Speed 5 0110 6 Position 6 110 6 Speed 6 0111 7 Position 7 111 7 Speed 7 1000 8 Position 8 1001 9 Position 9 Response Byte 1 command byte as received returned immediately Byte 2 OxOD after task completion Version1 0 rev 18 Feb 2015 25 ANDOR company SET SHUTTER STATE Open on Ext Trig Close Wheel A OxAB 51575 712 771 177217197 ae W
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