User Manual ADAM-MC
Contents
1. Coo 0 NON VIABLE _ NON vIABLE JX 74 Product Description 2 1 Packing List The ADAM is shipped with the following components Once you receive your instrument please check that all items listed below were shipped If any items are missing or damaged contact your local distributor or sales digital bio com Item Quantity Main device 1 AccuChip Kit Starter Kit Instruction Manual External video monitor Optional Installation CD Key Pad Power Cord USB cable Fuse N e e e e e FS FH After receiving ADAM examine it carefully for any damage incurred during transit Any damage claims must be filed with the carrier AN CAUTION Neglecting to remove any or all shipping brackets or foams prior to operation may result in damage to the equipment The shipping brackets or foam inserts must be reinstalled prior to shipping the unit to prevent damage to the equipment 8 si Digital Bio 10 Yt i fel wae Product Description 2 2 Identification of System Components Fig 1 View of the ADAM Control Button Door External video monitor ae a az I EE LCD Key Pad 1 Control buttons A Eject Ejects the chip holder from the Adam gt Run Start Performs all procedures of automatic counting Parking Protects the alignment of stage from external shock when the ADAM is moved to the other places is It is strongly recommended to park ADAM before2. 16 4 5 MENU setting lt lt lt lt lt lt lt roco oeooooiookoo oooiock c oinokosokoceER NSRSK 17 4 6 Result Analysis 20 4 7 _ MaIlNCENANMNCE lt Fosoioo gt o RGG GRE ae oo RRC RGG GARE 23 Section 5 Hardware 8 Software Installation 5 1 Connection between ADAM and computer 24 5 2 ADAM Report Software Installation 25 Section 6 ADAM Report Program Guide 6 1 ADAM Report Program Introduction 26 6 2 ADAM Report Program Function Guide 27 Appendix A Trouble Shooting 31 Appendix B Warranty gt a s nisnosetom cztaneccacec feUR nono Raan 32 Appendix C Technical Specifications 32 APPEND Product iS tases A ORA RAR AA Ga RR RA 34 Contact Iniommalo lt c srco lt F RFhoesEnechas oh oca aerate ease seas 35 8 si Digital Bio 7 Xadi 5 Introduction 1 1 Technology Mechanical Until now cell counting and viability measurement for many types of cells have been performed manually using hemocytometer with Trypan Blue exclusion method which is to distinguish viable cells from non viable cells One drawback of this method however is the propensity for the staining of artifacts another drawback is tha
3. 18 75 counting results of each frame that ADAM ka captures will be shown here Ti count 21 7 Ti count 2 2 Ti Auto Count 1030504 Ti error Frame 0 Ti Total 1604 Mi count 1 95 Mi count 2 64 Mi count 3 6 Mi count 4 92 Mi count 5 Mi count G x l Digital Bio 29 J ledle aid ADAM Report Program Guide Graph NONVIABLE 2 l iie INF III Mil WII III Wn EAE lhe ee ee ee ee L HH H RRARRRAARAAL th This section shows information of cell size distribution and counting results of each frame that ADAM captured Through cell size graph you can figure out whether there are cell clumps or aggregates In case of counting evenly distributed cells without any aggregation there should be a single peak on distribution of cell size The size of cell in graph is not real size of cell It is the size of pixels in fluorescence image captured by ADAM And the size information is only to judge if there is a lot of aggregated cells Vertical red line indicates the cut off size Counting results between first and second red line are counted as single cell and those between ill second and third line are counted as 2 cell and so on Any results before first red line will not be counted as cell Digital Bio 30 XK a a Trouble shooting Troubleshooting Table Problem ADAM does not power up Inaccurate result Software does not work When e
4. Korea Tel 82 2 6220 7728 Fax 82 2 6220 7721 NanoEnTek Inc USA 8 Europe 300 Washington St STE 416 Newton MA 02458 Tel 1 617 965 1414 Fax 1 617 965 1415
5. A1 1997 A2 2001 CCD camera B W CCD Filter Excitation filter Dichroic filter Emission filter Weight 9 Kg Size WxLxH 220 x 375 x 250 mm Degree of protection IPXO AccuChip Measuring range 5x 104 to 4 x 10 cells mL Analysis time 2 2 5 min test Loading sample vol 20 uL for AccuChip 2 12 uL for AccuChip 4 test Measuring vol 8 5 uL for AccuChip 2 3 uL for AccuChip 4 test AccuStain Solution T N PI propidium iodide staining of total cells an d non viable cells Accessories Power cord 1 5 m Fuse 250 VAC 3 A F3 15AL250V Keypad External video monitor optional Environment Condition 5 lt T lt 30C Altitude lt 2000 m 33 Aa Da Product List Cat No Adam MC ADM 001 AD2K 200 AD4K 200 ADR 1000 8 e Digital Bio Product Adam External video monitor optional AccuChip2 Kit Max 400 tests kit AccuChip Kit Max 800 tests kit AccuStain Max 1 000 tests kit Contents Quantity Main device 1 7 LCD Monitor 1 200 pcs AccuChip2 1 12 5 ml AccuStain solution T 2 1 12 5 ml AccuStain solution N 200 pcs AccuChip 1 12 5 ml AccuStain solution T 2 1 12 5 ml AccuStain solution N 12 5 ml AccuStain solution T 4 12 5 ml AccuStain solution N 2 34 Contact Information E Mail sales digital bio com Internet www digital bio com NanoEnTek Inc HQ 12F Ace High end Tower 235 2 Guro 3 Dong Guro gu Seoul 152 711
6. designed to manage and report all results from ADAM All measurement results are automatically saved on the memory of ADAM The user can download the data from the memory of ADAM and export it to Excel xls format The user can delete data from memory of ADAM or can save captured images into Desktop or Laptop hard drive The data list window consists of the sample number chip date time total nonviable viable viability counting result in CAUTION Before running the program check the connection of USB cable between the Adam and the laptop or desktop computer Image Number Ch Digital Bio rad rao Agra Qa h a fo r CX A Ci x h hs JE JE TE 25 25 JE dE 28 JE JE ab AE J ELA EPOK A cya ge 2003 01 08 2003 oF 2009 01 07 2003 01 06 2009 01 06 2009 01 06 2009 01 06 2009 01 06 2009 01 06 200 OK 2003 01 0E 2003 OK 2003 01 0E 2003 DE 2009 01 06 2005 DE JE HK be fe S S S Oo ki fo i io ta EE p D m oo uj 0 T 1 ro 1 D DI a 1 0 Nanviable 2 FSE5 Zee 3 53E5 Ar 4 98E5 ODED 0 00E0 ODECD viable L48E5 BE 17E4 4 0NIE4 D ODEGD O 00E0 1 20E3 ETSER Saved experiment 68 Read data Auto Save Delete data OFF Image path Temp viability count 15 79 46 x count 16 686 a7 count 17 9 21 m count 16 76 count 19 FF count 20 75 trzl 72 kute Count 1052000 error Frame O otal 163 Wi count 1 101
7. turning it off 2 Door Chip holder comes out here 3 LCD Displays the process and the result 4 Keypad Inputs the sample number and Enter button Less than 3 characters 5 External video monitor To see the actual cell shape and check if any clumped cell through this monitor 8 e Digital Bio u Jln Product Description 2 3 Identification of System Components continued Fig 2 Rear view of the ADAM Fan Power Switch Key Pad Port Power Plug RS232C Port USB Port Video Port Fan Adam s cooling fan Power switch Main power on off control Power plug Connect the ADAM power cord to wall outlet Video Port External video monitor port USB port Connect the USB serial cable to computer RS 232C serial port Not Connected Port for only QC and Service Key pad port Connect the Keypad NO OUT B W N 8 e Digital Bio 12 Tetley c System Installation 3 1 Environmental Requirements To insure correct operation and stable performance over an extended period of time install the ADAM in a location which meets the following conditions e Room temperature between 20 and 35 C Not recommended for cold room use 4 C CAUTION At low temperature 10C please warming up the system for 10 min e Not exposed to direct sun light e Not subject to direct or continuous vibration e Not subject to intense magnetic or electromagnetic fields e Relative humidity b
8. 8 e Digital Bio no N U O tora J __rorat__CJirz LOLON 4 iil Y NON VIABLE ll KW NON VIABLE N2 AccuChip4X 20 JX General Operation gt Error Messages 125 IEG DES L c Ee c OE 2 channel 4 channel This error message will show in case when frames with errors are E over 50 of total counting frame Frame with error is a frame that contains cells whose diameter is larger than 30um This error message will show in case when there are cells more than O 4X10 cells ml Over detection range will be shown on Report program This error message will show in case when there are cells more than H 2X10 cells ml High concentration cells will be shown on Report program This error message will show in case when there are cells less than L 4X10 gt cells ml Low concentration cells will be shown on Report program This error message will show in case when there are cells less than U 5X10 cells ml Under detection range will be shown on Report program 8 e Digital Bio 21 Jade General Operation Sample image of error message in Report program 2009 01 08 2009 01 08 2009 01 08 2009 01 08 2009 01 08 2009 01 08 Digital Bio Image The number of cells 1 0466 Cells mL The number of excepted images 0 The number of cells 1 0366 Cells mL The number of excepted images 9 Th
9. ADAM A New Standard of Automatic Cell Counter INSTRUCTION MANUAL p Digital Bio Developed and Manufactured by NanoEnTek Inc E Mail sales digital bio com Webpage http www digital bio com NanoEnTek Inc HQ 12F Ace High end Tower 235 2 Guro 3 Dong Guro gu Seoul 152 711 Korea Tel 82 2 6220 7728 Fax 82 2 6220 7721 NanoEnTek Inc USA 8 Europe 300 Washington St STE 416 Newton MA 02458 Tel 1 617 965 1414 Fax 1 617 965 1415 Copyright 2008 by NanoEnTek Inc All rights reserved Published in Korea H a Digital Bio All the materials in this manual are protected by Korean and international copyright laws They cannot be reproduced translated published or distributed without the permission of the copyright owner ADAM MC User s Manual Homepage www digital bio com E mail sales digital bio com Manufactured by m NanoEnTek Inc 12F Ace High end Tower 235 2 Guro 3dong Guro gu Seoul 152 711 Korea Tel 82 2 6220 7911 Fax 82 2 6220 7721 E Mail sales digital bio com The information in this manual is described as correctly as possible and is applicable to the latest firmware and software versions but it may be changed without prior consent or notification Copyright 2008 by NanoEnTek Inc All rights reserved Published in Korea Documentation NESMU AMC 002E V 1 5 Revision History Date October 2008 Date February 2009 Date June 2009 Date Se
10. Data list Information 26 Jade io ADAM Report Program Guide 6 2 ADAM Report Program Function Guide Image Auto Save OFF Image path ae Data list Information ys Digital Bio D Image frame Image captured by ADAM will be shown here Q Function Buttons Start cell counting saving images exporting data and all function of Report Program are handled by using these buttons see p 28 for more detailed information of each button 3 Data List All saved data in ADAM will be loaded and shown in data list section 4 Information Operation and counting results of each frame will be displayed here Graph Analysis of results including cell size and frame by frame counting will be shown in graph section XK Digital Bio 27 SJal a ADAM Report Program Guide Function Buttons Start cell counting mm Park Lock stage of ADAM Loads the experiment data from the memory of the main device Eject chip holder out of ADAM Deletes all of the loaded data and memory of the main device Transfers the data list to Excel format and saves it Auto Save Turn on or off automatic image save option OFF Default image save folder is C Program Files Digital Bio ADAM I mages Images will be saved until your hard drive has no more capacity to save Be sure to set Auto Save off unless you need to save images Example of saved image file 081221 yymmdd 203482 hh
11. Easy Total cells Q 3 ste ps l EELZLUE N Bogi Bio 8 e Digital Bio 16 Jad a General Operation 4 5 Menu setting You can set the menu as you press the button on the keypad from the screen for inputting cow numbers You can find the setting modes by selecting the number from the Menu 4 5 1 Chip Selection User can select the 2 kinds of chip type One is the two channel chip AccuChip2 Another is the four channel chip AccuChip Press 1 or 2 and Enter After pressing the Enter key the screen will return to the Menu screen automatically 8 i Digital Bio 17 Jade General Operation 4 5 2 Counting method You can change the capture frame mode Configuration U Counting Method Chip selection Counting method LCD contrast System info Method 1 1 High precision 2 High speed Counting Method 1 High precision 2 High speed Value is rerecorded Press i or 2 and Enter Method 1 2CH High Precision 60 frames Capture High Speed 30 frames Capture 4CH High Precision 22 frames Capture High Speed 11 frames Capture You can select the 60 or 30 frames in 2CH mode and 22 or 11 frames in 4CH mode for counting as well After pressing the Enter key the screen will return to the Menu screen automatically 8 e Digital Bio 18 XK XXa General Operation 4 5 3 LCD con
12. cumentation associated with its components Any use of the equipment in an unspecified manner is strongly discouraged and may result in damage or injury as cautioned by signed warnings 8 si Digital Bio Safety Symbols The following symbols are found on the instrument and this document Study the meaning of the symbols and always use the equipment in the safest possible manner A Caution amp Warning Oo Protective earth Ground S This instrument and consumables conforms to the Declaration of Conformity Caution Biohazard Protective measures must be used in dealing with biologically hazardous materials such as carcinogenic reagents 8 si Digital Bio Warnings Item Battery inside device Cover Manual Sample handling Waste Operator 8 e Digital Bio Warning Risk of explosion if battery is replaced by an incorrect type This battery is not replaceable by a user Referto a qualified personnel Do not remove a cover or dissemble a case There is no adjustable components inside the instrument If malfunction is found refer to a service personnel Do not attempt to service the equipment unless this manual has been consulted and is understood This manual is available in English only Failure to heed this warning may result in injury to service provider operator from electric shock mechanical or other hazards Wear gloves during sampling Users sample may have the infectious biohaza
13. e number of cells 1 01E6 Cells mL The number of cells 1 07E6 Cells mL The number of excepted images 0 number number number number number number number Saved esperiment 74 Data list of cells 1 04E6 Cells mL of excepted images 0 ot cells 1 03ZE6G6 Cells mL of excepted images 9 of cells 1 01EG Cells mL of cells 1 07E6 Cells mL of excepted images 0 Read data Delete data ath n Files Digital Bio ADAM Browse We LIIL M2 count Mz count Mz count ME count Me count MZ count ME count Me count Mz count NE Kuko Count 1074799 Ne error Frame 0 M2 Total 1646 Information Jade General Operation 4 7 Maintenance and Cleaning ADAM does not need regular maintenance ADAM has no replacement of consumable materials Clean the exposed outer surface of ADAM using a soft cloth and isopropyl alcohol or deionizes water A CAUTION Dispose of wipes in an appropriately labelled solvent contaminated waste container 8 e Digital Bio 23 X ed Hardware 8 Software Installation 5 1 Connection between ADAM and computer The following steps will guide you to connect USB cable 1 Connect the USB cable to ADAM 2 Connect the USB cable to Desktop or Laptop computer 3 Turn on ADAM and Desktop computer F r ia Key pad port XQ sin 8 e Digital Bio 24 Xadi Hardware amp Software Insta
14. e thoroughly by vortexing 3 This subsequent cell lysate 20 ul for AccuChip 2X 12 ul for AccuChip 4X is loaded into the N1 or N2 channel When you load 12 ul of the cell lysate into the N channel please be careful not to make bubbles T channel is the upper part of chip and N channel is the lower part of the chip as below ST T1 TOTAL IU TOTAL JL T2 gt HJ m Onnee onv QJ N2 AccuChip2 AccuChip 8 si Digital Bio 15 Jade General Operation 4 4 Operating the ADAM 1 Press EJECT button on the main device to eject the chip holder 2 Insert the Chip loaded with the sample onto the chip holder Please be careful not to make bubbles 3 Press the P Run button on the main device 4 Automatic Focus will be carried out at the first time the device is booted Once ADAM have done the Auto Focus process and on the following time focusing process will be skipped 5 After calculating the cell number the chip will be ejected automatically Then chip can be removed 6 The calculated cell number per iml will be displayed automatically 7 For another experiment repeat the process from steps 1 5 8 Operator should remember the above procedure to perform the automatic cell counting with ADAM 2x 1 Mix sample with dye solution 7 amp loading the sample 2 Channel Chip 20 pl 4 Channel Chip 12 ul L 3 Quick result GI m ple amp
15. etween 0 95 e Area free from corrosive gases or other corrosive substances e Area with very little dust or other airborne particles e Allow a 10 cm minimum space around the instrument for proper air flow e Not allow to put heavy material on top of ADAM 3 2 Power on and Initial Display 1 Check the connection of the main device power cord 2 Make sure that the main power switch is in the I ON position On the rear side of the main device When you turn on the ADAM it will go through self diagnostic tests including all optical components If a problem is detected please contact your local distributor or sales digital bio com If boot up is successful and no errors are detected the home screens will be displayed as below a B H TD ID2 ADAM MC Digital Bio Press A to load eootine Wy unload Press gt to start 8 si Digital Bio 13 JX c System Installation 3 3 Icon Functions A ba in EF H LHOU gt Display error message E E ID1 123 ID2 456 Display a status of the performance such as eee eject run parking or insert Ti 1 12 E6 Ni 0E6 Status of the Chip Holder T2 1 12 E6 N2 T1I N1 V T2 N2 V Display menu setting Icon Features Shows when cell counting is running After you press the run button Shows the Chip Holder is ejected After you press the eject button Shows the Chip Holder is parked After you press the park button Shows t
16. he Chip Holder is inserted Shows that performance setting is high precision ADAM scans 22 fields in each cham ber representing a total volume of 3 1 ul Shows that ADAM reads 4 Channel chip 8 si Digital Bio 14 Testes General Operation 4 1 Preparing cell 1 Cultivate the required number of cells 2 Aspirate the media and rinse the flasks using PBS or DBPS 3 Aspirate the PBS and add Trypsin EDTA 4 Neutralize the trypsin by adding medium containing serum 5 Add an appropriate volume of growth media or PBS to dilute to a final concentration of 5 x 104 cells ml to 4 x 10 cells ml zs Concentration out of this range will result in errors Refer to page 21 or more information on errors 4 Thoroughly mix the cell pellet by vortexing 5 Check visually if any cell clumps or agglomerates are remained 4 2 Counting Total Cell 1 The cell sample 50 ul should be mixed with an equal volume of AccuStain Solution T 50 ul for total cell counting 1 to 1 dilution 2 Mix the sample thoroughly by vortexing 3 This subsequent cell lysate 20 ul for AccuChip 2 12 ul for AccuChip 4 is loaded into the T1 or T2 channel When you load 12 ul of the cell lysate into the T channel please be careful not to make bubbles 4 3 Counting Non viable Cell 1 The cell sample 50 ul should be mixed with an equal volume of AccuStain Solution N 50 ul for Non viable cell counting 1 to 1 dilution 2 Mix the sampl
17. llation 5 2 ADAM Report Software Installation To install the Adam Report software follow the directions as below 1 Insert the installation CD ROM into the computer Then open the file Setup_ADAM_v1 x x x exe Report program can be installed in Windows 2000 XP or higher version The start up dialogue of the software as shown below will appear Click Next to start installation 2 If you want to change installation folder click Browse and choose the location that you want After choosing installation folder click Install to proceed with the installation The computer activates the Installation of the Software nitial installation folder is C Program Files Digital Bio ADAM 3 Report Program will be installed automatically 4 Click Ok to finish the installation If the installation was successful the report program can be found at Start gt All Program gt ADAM A x INST ADAM Operation software vie U0 peta yare v1 0 0 0beta LO K This will install ADAM Operation Software v1 0 0 0 beta on your computer It is recommended that you exit all other programs before proceed with installation Click lt Next gt to continue installation Or click lt Cancel gt to cancel installation Copyright c Digital Bio Technology 2006 XK Digital Bio 25 Jestes s ADAM Report Program Guide 6 1 ADAM Report Program Introduction This Report Program is
18. mmss N1 channel name 002 bmp Choose folder to save images automatically 8 e Digital Bio 28 Jade io ADAM Report Program Guide 3 Data List Humber Chip Pate l Te L Ta _ Nonviable EE e eTA 5 34 58 BS TES 2 606 4 Foose 1 2rfi4d 5 34 58 s r Ee r 15 E4 z00zrizri1i 08 05 i f GES 2 r1ES z00zyrizrii 08 05 3 1 7E5 5 39E4 2002 12 03 FAE AEA 26E Cie AED 0038 12 03 2O 23 0 4 05E5 6 OOE4 200g aes of 20 20 15 4 29E 3 65E5 20 20 15 4 02E5 6 fobs 20 17 30 Z 45E C JE su 20 17 30 4 01E5 7 16E4 2O 14 44 3 96E Z JGES 20 14 44 4 1 3E5 6 96E4 2008 3 12 09 ia As oe ES A Si Cig ay lee 2008 12 03 20 11 12 4 09E5 E 16E4 2 oosfizfog 70 09 31 7 69E 3 06E5 2008 gt of 20 09 31 4 z 65 1864 20 07 49 oP gni i 3 08E5S 2008 ZE oo 20 07 49 zZ005 12Z 03 z006 08 EA Rt ea Ie TET re AD et Oe OL Da Re a Naa et se a ae Ul a PANE EEE experiment 120 Data list Data list shows data stored in ADAM memory Total amount of stored results are indicated at bottom of list as Saved experiment Up to 200 counting results are automatically saved to ADAM memory When memory of ADAM is full new counting result will replace old data These data can be exported as Excel Sheet xls and stored in personal computer or can be erased from ADAM memory 4 Information Se ate This section shows information regarding T1 count zp RA EE operation of ADAM If cell counting is K started through Report Program the Ti count
19. ptember 2009 Date January 2010 lt lt lt lt lt oS We O O V1 O CO 8 si Digital Bio Safety Precautions 1 Always ensure that the power supply input voltage match the voltage available in your location For operation environment See Appendix C Technical Specification This machine is air cooled so its surfaces become hot during operation When installing it leave a spaces of more than 10 cm 4 inches around it 4 Never insert metallic objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage 5 Always set the main switch on the power supply unit to C OFF before connecting the power cord to the wall outlet 6 Always ensure that the grounding terminal of the instrument and that of the wall outlet are properly connected The power cord should be connected to a grounded 3 conductor power outlet 7 To avoid potential shock hazard make sure that the power cord is properly grounded 8 Do not position the equipment so that it is difficult to operate the disconnecting device 9 Be sure to set the main switch to OFF unplug the power cord and lock the stage before moving 10 If the instrument is broken or dropped disconnect the cord and contact a authorized service person Do not disassemble the instrument 11 Use only authorized accessories 12 Use this equipment only as specified in this manual and as specified in any do
20. rdous substance After using Accuchips appropriately dispose it as biohazardous waste Do not reuse the Accuchips Must have the general knowledge of cell counting procedure and bio safety to handle the sample that may have the infectious biohazardous substance Date Aug 01 2008 Aug 01 2008 Aug 01 2008 Aug 01 2008 Aug 01 2008 Aug 01 2008 Table of Contents Page Section 1 Introduction 1 1 Technology Mechanical 1 2 Technology Viability Measurement 9 Section 2 Product Description 2 1 Packing List 10 2 2 Identification of System Components view of the ADAM 11 2 3 Identification of System Components rear view of the ADAM 12 Section 3 System Installation 3 1 Environmental Requirements 13 3 2 Power on and Initial Display 13 523 CON FUNCUIIONS lt F lt eeos FG F OGG GN GG ERZGSAWENEGKA SE RAR SAN KORZGGAGEGARKAK 14 Section 4 General Operation 4 1 Preparing cell mixture 15 4 2 Counting Total Cell 15 4 3 Counting Non viable Cell 15 4 4 Operating the ADAM
21. rror message is shown For information on each error message see page 19 8 e Digital Bio Cause Power switch in off position No power from outlet Bad power cord Cell number may be out of range AccuStain Solution has expired Too high clumped cells PC setup incorrect wrong instruct mode Cable s not fully connected wrong adaptor When there are too many frames with errors Error message E When too many cells are loaded Error message O High concentration of cells Error message H Low concentration of cells Error message L Too few cells are loaded Error message U Solution Check power switch on back of unit Check power source Replace Adjust the number of cells between 5X104 4X10 cells ml refer to page 21 Check the expired data Try again after vortexing the cells Check program setup Check all connections Check the suspension of cells if all cells are fully dissociated into single cells If contaminants except cells are found prepare sample again Check if concentration of cell is too high Dilute the sample and count again Check if concentration of cell is high or not Dilute sample and count again Check if concentration of cell is low or not Use concentrated sample and count again Check if concentration of cell is too low Use concentrated sample and count again 31 s 2 11 9 7 Warran
22. t the naked eye can only differentiate between cells in a limited concentration range in the hemocytometer chamber This combined with the potential problem of cell aggregation and limited sample volume leads to the common variation of counts normally associated with this method To address these problems Digital Bio has developed the ADAM which is based on a fluorescent microscopy technique for counting cells The ADAM utilizes sensitive fluorescence dye staining LED optics and CCD detection technologies to make the cell analysis more accurate and reliable To count cells using ADAM the cells are mixed with a Propidium Iodide PI stain and directly pipetted on to a disposable plastic chip The chip is then loaded onto a precision stage An ADAM system is automatically focused onto the chip and cells that have been stained are recorded by a sensitive CCD camera The image results are automatically processed generating the cell count which is displayed on the front of the instrument Simple Fast Accurate Reliable Ach Chip gt Objective lens ITI Excitation filter _S 5 Dichroic filter 2 filter CCD camera Green LED Mirror 8 si Digital Bio Xadi i Introduction 1 2 Technology Viability Measurement Adam is based on staining mammalian cell DNA with a fluorescent dye Propidium Iodide PI PI does not enter cells with intact cell membranes or active metabolism In contrast cells wi
23. th damaged membranes or with inactive metabolism are unable to prevent PI entering the cell As a result the nuclei of non viable cells will only be stained The Adam provides two kinds of staining solutions AccuStain Solution T for the total cell counting is composed of the fluorescent dye PI and lysis solution AccuStain Solution N for the non viable cell counting is composed of the fluorescent dye and PBS In order to measure the total concentration of cells the plasma membranes of all the cells must be disrupted to stain all the Nuclei with PI The process of disrupting and staining is achieved by treatment with AccuStain Solution T In the second solution live cells remain intact and are not stained Only the non viable cells are stained and detected After treatment the prepared cells will be loaded into the chip The viability will be automatically calculated in the Adam MC software after each measurement of the total cells and the non viable cells Lysis soln i Viable cell e l Lysis soln e PI Non viable cell Viable cell Non viable cell 8 e Digital Bio PI stained Nuclei PI stained Nuclei Viable cell PI stained Cell Total Cell Count A TOTAL l TOTAL AJ p 1 4 4 A ID NON VIABLE NON VIABLE I A B X 100 Viability A Non viable Cell Count B IY TOTAL Ee ToTaL r
24. trast Press the number 4 key to adjust the brightness of the LCD screen from the MENU ES Adjust LCD contrast if the letters do not appear clearly on the screen Press the Enter key after you input the three digits The range is from 600 to 750 After pressing the Enter key the screen will return to the Menu screen automatically 4 5 4 System Information The device versions and date which have been installed in the device can appear when the number 5 key is selected from the MENU After pressing the Enter key the screen will return to the Menu screen automatically Digital Bio 19 feidfe s General Operation rc Press the key after menu setting Once inputted the screen will return to the counting mode automatically 4 6 Result Analysis A ID1 EHH 123 ID2 456 T1 1 10 E6 Ni 5 50 E5 T2 2 20 E6 N2 5 50 E5 TI N1 5 50E5 V 50 T2 N2 1 65E6 V 75 Sample name ID1 123 ID2 456 T2 2 20E6 N2 5 50E5 75 1 10E6 1 10 X 10 cells ml Number of Number of Viabilit Total cell Non Viable cell y T1 1 10E6 N1 5 50E5 50 The viability will be automatically calculated by the Adam software after each measurement of the total cells and the non viable cells First the total cell number and second non viable cell number were measured and then the cell viability is calculated as subtracting non viable cell counting numbers from total cell counting ID 1
25. ty Digital Bio warrants that the ADAM will be free from defects in material and workmanship for a period of one 1 year from date of purchase If any defects occur in the ADAM during this warranty period Digital Bio will repair or replace the defective parts at its discretion without charge The following defects however are specifically excluded 1 2 NO O71 A W Defects caused by improper operation Repair or modification done by anyone other than Digital Bio or an authorized agent Damage caused by substituting alternative parts Use of fittings or spare parts supplied by anyone other than Digital Bio Damage caused by accident or misuse Damage caused by disaster Corrosion caused by improper solvent or sample For your protection items being returned must be insured against possible damage or loss Digital Bio cannot be responsible for damage incurred during shipment of a repair instrument It is recommend that you save the Original packing material in which the instrument was shipped This warranty should be limited to the replacement of defective products For any inquiry or request for repair service contact sales digital bio com or your local distributor 8 si Digital Bio 32 M el dle Technical Specifications 8 e Digital Bio ADAM Voltage AC100 240 V 50 60 Hz Current max 1 8 A max 100 W Fuse F3 15AL250V Objective lens 4 X LED 4W Green LED IEC 60825 1 1993
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