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Agilent 1200 Series High Performance Autosamplers & Micro Well

1. Figure 3 Bypass Position The standard injection starts with draw sample from vial In order to do this the needle moves to the desired sample vial position and is lowered into the sample liquid in the vial to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle is then raised again and moved onto the seat to close the sample loop In case of an injector program several steps are interspersed at this point Figure 4 Drawing the Sample 1200 Series Autosamplers User Manual 13 1 14 Introduction Flush the Needle Before injection and to reduce the carry over for very sensitive analysis the outside of the needle can be washed in a flush port located behind the injector port on the sampling unit As soon the needle is on the flush port a peristaltic pump delivers some solvent during a defined time to clean the outside of the needle At the end of this process the needle returns to the injection port Inject and Run The final step is the inject and run step The six port valve is switched to the main pass position and directs the flow back through the sample loop which now contains a certain amount of sample The solvent flow transports the sample onto the column and separation begins This is the beginning of a run within an analysis In this stage all major performance influencing hardware is flushed internally by the solvent fl
2. to waste to column Figure 16 Hydraulic Connections 50 1200 Series Autosamplers User Manual Installing the Autosampler 3 Installing the Sample Tray Press the bottom on the right side to release the front door Lift the front door Load the sample tray with sample well plates and vials as required Aa O N Slide the sample tray into the autosampler so that the rear of the sample tray is seated firmly against the rear of the sample tray area 5 Press the front of the sample tray down to secure the tray in the autosampler If the tray pops out of position the air channel adapter is not correctly inserted 1200 Series Autosamplers User Manual 51 52 Installing the Autosampler Figure 17 Installing the Sample Tray 1200 Series Autosamplers User Manual Installing the Autosampler 3 Transporting the Sampler When moving the autosampler inside the laboratory no special precautions are needed However if the autosampler needs to be shipped to another location via carrier ensure e The transport assembly is in the park position Use the Lab Monitor and Diagnostic software or the Instant Pilot for this command e The vial tray and the sample transport mechanism is secured with the transport protection foam 1200 Series Autosamplers User Manual 53 3 Installing the Autosampler Transporting the Sampler 54 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual e 4 U
3. gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the power cord designed for your region Use of unsupplied cables The use of cables which haven t been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 1200 Series Autosamplers User Manual 25 2 Site Requirements and Specifications Bench Space The autosampler dimensions and weight see Table 3 on page 27 allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 2 5 cm 1 0 inch of space on either side and approximately 8 cm 3 1 inches at the rear for the circulation of air and room for electrical connections Ensure the autosampler is installed in a horizontal position The thermostatted sampler dimensions and weight see Table 3 on page 27 allow the instrument to be placed on almost any laboratory bench The instrument requires an additional 25 cm 10 inches of space on either side for the circulation of air and approximately 8 cm 8 1 inches at the rear for electrical connections Ensure the autosampler is installed in a horizontal position If a complete Agilent 1200 Series system is to be installed on the bench make sure that the benc
4. 2 pl volume Measured with injections of benzylalcohol Up to 600 bar 8700 psi 0 2 5 cp 2 x well plates MTP 10 x 2 ml vials 108 x 2 mL vials in2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 0mL in 2 x 27 Eppendorf tube plate Also compatible with the Agilent 1200 Series sample capacity extension for further expansion of the sample capacity 1200 Series Autosamplers User Manual 29 2 30 Site Requirements and Specifications Table5 Performance specifications Agilent 1200 Series High Performance Autosampler SL Injection cycle time Carry over Typically lt 17 s using following standard conditions Default draw speed 100 pl min Default eject speed 100 pl min Injection volume 5 ul Typically lt 0 004 using the following conditions Column Agilent Zorbax SB C18 21x50mm 1 8um P N 827700 902 Mobile Phase A H20 0 05 TFA B ACN 0 045 TFA Gradient 0 1min 10 B 3 1 min 90 B 3 2 min 90 B 3 21 10 B 4 5min stop Flow rate 0 5 ml min Temperature 25C Wavelength 257 nm Sample 1200 ng ul Chlorhexidine disolved in H20 with 0 1 TFA Jul injected and measure both on Agilent 6410 QQQ and G1315C DAD Wash solution H20 with 0 1 TFA 5 sec Table6 Performance Specifications Agilent 1200 Series Micro Well Plate Autosampler Type Specification GLP features Communications
5. Draw speed pl Eject speed pl High performance autosampler Default value 200 200 Minimum 10 10 Maximum 1000 1000 High performance autosampler SL Default value 100 100 Minimum 4 4 Maximum 1000 1000 Micro Well Plate Autosampler with 8 pl loop capillary Default value 4 10 Minimum 0 7 0 7 Maximum 20 100 Micro Well Plate Autosampler with 40 pl loop capillary Default value 4 10 1200 Series Autosamplers User Manual 77 78 5 Optimizing Performance Table 19 Draw and eject speed Draw speed pl Eject speed pl Minimum 0 7 0 7 Maximum 250 250 1200 Series Autosamplers User Manual Optimizing Performance 5 Choice of Rotor Seal Vespel Seal The standard seal has sealing material made of Vespel Vespel is suitable for applications using mobile phases within the pH range of 2 3 to 9 5 which is suitable for the majority of applications However for applications using mobile phases with pH below 2 3 or above 9 5 the Vespel seal may degrade faster leading to reduced seal lifetime Tefzel Seal For mobile phases with pH below 2 3 or above 9 5 or for conditions where the lifetime of the Vespel seal is drastically reduced a seal made of Tefzel is available Tefzel is more resistant than Vespel to extremes of pH however is a slightly softer material Under normal conditions the expected lifetime of the Tefzel seal is shorter than the Vespel seal however Tefzel may have the longer lifetime under more extrem
6. Features Increased pressure range up to 600 bar enabling the use of today s column technology sub two micron columns with the Agilent 1200 Rapid Resolution LC System Increased robustness by optimized new parts high speed with lowest carry over by flow through design increased sample injection speed for high sample throughput increased productivity by using overlapped injection mode and flexible and convenient sample handling with different types of sample containers such as vials and well plates Using 384 well plates allows you to process up to 768 samples unattended The G1377A Agilent 1200 Series Micro Well Plate Autosampler is designed to perform capillary LC with injection of sample volumes ranging from nL to uL 8 1200 Series Autosamplers User Manual Introduction 1 Features A micro Rheodyne valve and the optimized design of the needle seat loop and seat capillaries minimize dispersion A high resolution metering device offers resolution ten times better than a standard autosampler bypass operation facilitates low delay volume increased sample injection speed for high sample throughput flexible and convenient sample handling with different types of sample containers Using 384 well plates allows to process up to 768 samples unattended Technical Principle The well plate sampler transport mechanism uses an X Z theta robot to optimize the positioning of the sampling arm on the well plate Once the sampling arm is positi
7. Safety features Injection range Precision Pressure range Sample viscosity range Sample capacity Early maintenance feedback EMF electronic records of maintenance and errors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 01 8 ul in 0 01 pl increments with the small loop capillary 0 01 40 ulin 0 01 pl increments with the extended loop capillary Typically lt 0 5 RSD of peak areas from 5 40 ul Typically lt 1 RSD from 1 5 ul Typically lt 3 RSD from 0 2 1 pl up to 400 bar 5880 psi 0 2 5 cp 2 x well plates MTP 10 x 2 ml vials 108 x 2 mL vials in 2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 0 mL in 2 x 27 Eppendorf tube plate 1200 Series Autosamplers User Manual Site Requirements and Specifications 2 Table6 Performance Specifications Agilent 1200 Series Micro Well Plate Autosampler Type Specification Injection cycle time Carry over Typically lt 30 s using following standard conditions Default draw speed 4 pl min Default eject speed 10 pl min Injection volume 0 1 pl Typically lt 0 05 using the following conditions Column 150 x 0 5 mm Hypersil ODS 3 pm Mobile phase Water
8. 150 1200 Series Autosamplers User Manual Index Index A accessory kit micro well plate sampler 134 accessory kit high perf autosampler 133 advanced operating modes 20 Agilent Lab Monitor amp Diagnostic Software 84 Agilent on internet 150 150 air circulation 26 algae 149 149 autosampler accessory kit contents 35 autosampler accessory kit contents 134 129 30 autosampler accessory kit 35 autosampler accessory kit 35 battery safety information 145 bench space 26 bench space 26 bypass 12 C cable connecting modules 39 capillaries 49 choice of vials and caps 76 cleaning 100 condensation 26 26 D delivery checklist 34 34 draw speed 76 76 DRAW 76 E ejectspeed 76 76 EJECT 76 EMF counters 103 setting 104 using 104 environment 24 26 26 error messages 85 ESD electrostatic discharge strap 99 F failure 85 flow connections 49 flow cell 149 solvent information 149 fuses 24 G G1373A 137 l information on lithium batteries 145 injection valve 16 injection volume precision 76 injection volumes less than 2 ul 76 injection sequence 12 injection valve 15 8 injection volume 76 injection volumes 76 installing the autosampler 1200 Series Autosamplers User Manual flow connections 49 interface cables 42 power cable 42 safety 42 sample trays 56 installing the sample tray 51 installing the thermostatted autosampler interface cables 45 power cable 45 preparation 46 safe
9. Change Needle Seat function can be found under the Tools icon Open the front door and remove the side door Remove the plate tray from the tray base 4 Disconnect the seat capillary from the injection valve port 5 with the 1 4 inch wrench Use the flat head screwdriver to remove the needle seat Insert the new needle seat assembly G1367 87101 Press it firmly in position Connect the seat capillary to the injection valve port 5 with the 1 4 inch wrench Replace the plate tray in the tray base Re install the side door and close the front door In the user interface close the Change Needle Seat function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found under the Tools icon 1200 Series Autosamplers User Manual Maintenance 7 Exchange the Needle Seat G1367 87105 on the G1367D Exchange the Needle Seat G1377 87101 on the G1377A When When the seat is visibly damaged When the seat capillary is blocked Tools required e 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Flat screwdriver Parts required Partnumber Description G1367 87105 Needle Seat without capillary for G1367D G1367 87303 Optional Seat Capillary 150 mm 0 12 mm ID for G1367 87105 Needle Seat G1367 87302 Optional Seat Capillary 150 mm 0 17 mm ID for G1367 87105 Needle Seat G1377
10. This is useful during troubleshooting where close observation of each of the sampling steps is required to confirm a specific failure mode or verify successful completion of a repair Each injector step command actually consists of a series of individual commands that move the autosampler components to predefined positions enabling the specific step to be done Table 21 Step Commands Step Action Comments Valve Bypass Plunger Home Needle Up Move to Location Needle into sample Draw Needle Up Needle into Seat 1200 Series Autosamplers User Manual Switches injection valve to the bypass position Moves the plunger to the home position Lifts the needle arm to the upper position Move the needle arm to the vial location on the plate Lowers the needle into the vial Metering device draws the defined injection volume Lifts the needle out of the vial Lowers the needle arm into the seat Command also switches the valve to bypass if it is not already in that position Command lifts the needle and lowers the needle into sample Command can be done more than once maximum draw volume of 40 100 5000 ul cannot be exceeded Use Plunger Home to reset the metering device 91 6 92 Troubleshooting and Diagnostics Table 21 Step Commands Step Action Comments Valve Mainpass Switches the injection valve to the mainpass position Needle Up Mainpass Lifts the needl
11. 4 Push the loop capillary into the loop capillary protection tube until it comes out on the sampling unit side 5 Tighten the loop capillary fitting to the analytical head Install the loop capillary corrugated waste tube over the loop capillary 7 Pinch the holder clamp and reinsert the needle assembly into the needle carrier 8 Lock the needle tighter lock system 108 1200 Series Autosamplers User Manual Maintenance 7 9 Push the black chain into the needle assy until the stop 10 Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the autosampler is calculated from the needle pusher position 11 Remove the silicon safety tube from the needle 12 Replace the plate tray in the tray base Re install the side door and close the front door 13 In the user interface close the Change Needle Seat function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found in the Tools icon 1200 Series Autosamplers User Manual 109 7 Maintenance Removing the Needle Carrier Assembly When When the needle carrier is defect Tools required 2mm hex key 8710 2438 supplied in accessory kit Parts required Partnumber Description 1 G1367 60010 Needle Carrier assembly
12. 40 to 70 C 4 to 158 F Humidity lt 95 at 25 to 40 C 77 to 104 F Non condensing Operating Altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For storing the autosampler Safety standards IEC CSA UL Installation Category II Pollution Degree 2 1200 Series Autosamplers User Manual 27 2 Site Requirements and Specifications Performance Specifications Table 4 Performance specifications Agilent 1200 Series High Performance Autosampler Type GLP features Communications Safety features Injection range Precision Pressure range Sample viscosity range Sample capacity Injection cycle time Carry over Specification Early maintenance feedback EMF electronic records of maintenance and errors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 100 pl in 0 1 pl increments Up to 1500 ul with multiple draw hardware modification required Typically lt 0 25 RSD from 5 100 ul Typically lt 1 RSD from 1 5 pl variable volume 400 bar 5880 psi 0 2 5 cp 2 x well plates MTP 10 x 2 ml vials 108 x 2 mL vials in2 x 54 vial plate plus 10 additional 2 mL vials 30 x 6 mL vials in 2 x 15 vial plate plus 10 additional 2 mL vials 54 Eppendorf tubes 0 5 1 5 2 0mL in 2 x 27 Epp
13. 5183 4504 5183 4507 Clear glass 2 5182 0546 5183 4505 5183 4508 write on spot Amber glass 2 5182 0545 5183 4506 5183 4509 write on spot Table 14 Screw Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0714 5183 2067 5183 2070 Clear glass 2 5182 0715 5183 2068 5183 2071 write on spot Amber glass 2 5182 0716 5183 2069 5183 2072 write on spot 1200 Series Autosamplers User Manual 59 4 Using the Autosampler Table 15 Crimp Caps Description Septa 100 Pack Silver aluminum Clear PTFE red rubber 5181 1210 Silver aluminum Clear PTFE red rubber 5183 4498 1000 Pack Blue aluminum Clear PTFE red rubber 5181 1215 Green aluminum Clear PTFE red rubber 5181 1216 Red aluminum Clear PTFE red rubber 5181 1217 Table 16 Snap Caps Description Septa 100 Pack Clear polypropylene Clear PTFE red rubber 5182 0550 Blue polypropylene Clear PTFE red rubber 5182 3458 Green polypropylene Clear PTFE red rubber 5182 3457 Red polypropylene Clear PTFE red rubber 5182 3459 Table 17 Screw Caps Description Septa 100 Pack Blue polypropylene Clear PTFE red rubber 5182 0717 Green polypropylene Clear PTFE red rubber 5182 0718 Red polypropylene Clear PTFE red rubber 5182 0719 Blue polypropylene Clear PTFE silicone 5182 0720 Green polypropylene Clear PTFE silicone 5182 0721 Red polypropylene Clear PTFE silicone 5182 0722 60 1200 Series Autosamplers User Manual Using the
14. Autosampler 4 Configure Well Plate Types If the plate you are using is not found on the List of Recommended Plates and Closing Mat on page 57 you may configure a custom plate Measure the exact dimensions of the plate as marked below and enter the values in the plate configuration table of the ChemStation tas column y KF w gt D gt gt SE a S LRQA r IRS A E A B 7 NOTOW S J gt Ne a 4 p D S Figure 19 Well Plate Dimensions straight 1200 Series Autosamplers User Manual 61 4 62 Using the Autosampler y kF h E Ws nee B H a e J gt t lt t T pS Figure 20 Well Plate Dimensions staggered Table 18 Well Plate Dimensions Location Description Definition Limits Rows Number of rows on the plate up to 16 Columns Number of columns on the plate up to 24 Volume Volume in pl of a sample vessel A Row distance Distance in mm between the center of two rows B Column distance Distance in mm between the center of two columns C Plate length X size in mm at the bottom of the 127 75 0 25 mm plate SBS Standard 1200 Series Autosamplers User Manual Using the Autosampler 4 Table 18 Well Plate Dimensions Location Description Definition Limits D Plate width Y size in mm at the bottom of the 85 50 0 25 mm SBS plate Standard E Plate height Size in mm from the bottom to the up to 47 mm top of the plate F Row offset Distance in mm from the back
15. Autosamplers User Manual Installing the Autosampler Flow Connections to the Sampler Parts required Preparations Description 1 Parts from the accessory kits see Accessory Kits on page 34 Sampler is installed in the LC system WARNING When opening capillary or tube fittings solvents may leak out The handling of toxic and hazardous solvents and reagents can hold health risks 3 gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Connect the pump outlet capillary to port 1 of the injection valve 2 Connect column compartment inlet capillary to port 6 of the injection valve 3 Connect the corrugated waste tube to the seat adapter and the solvent waste from the leak plane 4 Ensure that the waste tube is positioned inside the leak channel 5 Drive the tube from the peristaltic flush pump to the solvent bottle in the solvent cabinet 6 Seat capillary see recommendations in Choice of Seat Capillary on page 80 1200 Series Autosamplers User Manual 49 3 Installing the Autosampler Flow Connections to the Sampler from pump Corrugated tube CT Loop capillary waste tube z gt Loh oth 4 t18 su
16. Maintenance 7 Exchange the Seat Capillary on the G1377A and G1367D When When the seat is visibly damaged When the seat capillary is blocked Tools required 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Flat screwdriver Parts required Partnumber Description G1375 87317 Seat Capillary 150 mm 0 10 mm ID for G1377 87101 Needle Seat G1375 87316 Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat G1375 87300 Seat Capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat G1377 87101 Optional Needle Seat without capillary for G1377A G1367 87303 Seat Capillary 150 mm 0 120 mm ID for G1367 87104 Needle Seat G1367 87302 Seat Capillary 150 mm 0 170 mm ID for G13767 87104 Needle Seat G1367 87105 Optional Needle Seat without capillary for G1367D n c E 1 Disconnect the seat capillary from the injection valve port 5 with the 1 4 5 16 inch wrench 2 Remove the needle seat see Exchange the Needle Seat G1367 87105 on the G1367D Exchange the Needle Seat G1377 87101 on the G1377A on page 113 3 Use the seat capillary mounting tool provided in the accessory kit and replace the seat capillary from the seat with the 4 mm wrench 4 Install the seat assembly in its location and reconnect the capillary to the injection valve port 5 5 Follow the procedure in Exchange the Needle Seat G1367 87105 on the G1367D Exchang
17. Seal and Plunger on page 119 See Removing the Loop Capillary on page 121 See Peristaltic Pump on page 125 1200 Series Autosamplers User Manual 105 7 Maintenance Removing the Needle Assembly When Tools required Parts required When the needle is visibly damaged When the needle is blocked Two 1 4 inch 5 16 inch wrenches 8710 0510 supplied in accessory kit 4 mm open end wrench 8710 1534 supplied in accessory kit Partnumber Description 1 1 G1367 87202 Needle assembly for G1367B D G1377 87201 Needle assembly for G1377A Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful when you remove the needle assembly gt Use the silicon safety tube supplied with every new needle 106 In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Seat function can be found in the Tools icon Open the front door and remove the side door 3 Remove the plate tray from the tray base gt on oI Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new needle over the needle Unlock the needle tighter lock system Loosen the loop capillary fitting on the analytical head side Remove the loop capillary corrugated waste tube Pinch the holder clamp pull back and remove the
18. WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be carefull when you remove the needle carrier assembly gt Use the silicon safety tube supplied with every new needle 1 In the user interface start the maintenance mode and select the Change Needle Carrier function In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Carrier function can be found in the Tools icon 2 Open the front door and remove the side door 3 Remove the plate tray from the tray base 4 Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new needle over the needle 5 Pinch the holder clamp pull back and remove the needle assembly from the needle carrier 6 Unplug the flex board on the sample transport 7 Unscrew the three holding hex screws with the 2 mm hex key Remove the needle carrier assy 110 1200 Series Autosamplers User Manual Maintenance 7 Installing the Needle Carrier Assembly When When the needle carrier is defect Tools required 2mm hex key 8710 2438 supplied in accessory kit Parts required Partnumber Description i G1367 60010 Needle Carrier assembly Install a new needle carrier G1367 60010 on place Install the three holding hex screws with the 2 mm hex key Plug in the flex board on the sample transport Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new need
19. after gradient is flushed out Having optimized these parameters further reduction of cycle times can be obtained if column equilibration is short compared to injector preparation or if automated column regeneration is configured Overlapped Injection during run mode decreases this time between runs But note that carry over and discrimination may increase dramatically doing so 1200 Series Autosamplers User Manual Optimizing Performance 5 Overlapped Injection after gradient is flushed out In this process the injection can be done in parallel to column equilibration phase without compromising any of the Autosampler s specifications This mode has one parameter The time when to start the overlapped injection defined as time after begin of run Considering a composition gradient that ends after 1 min with reestablishing starting conditions the overlap time has to be set to somewhat above 1 min to let the pump fill also the Autosampler s loop with start conditions Overlapped Injection during Run In this process as soon as the sample has reached the column the injection valve is switched back to bypass and the next injection cycle is performed except for switching the injection valve to mainpass This is done after actual run is finished and next analysis is started Doing so the sample preparation time is saved as parallel to the run Switching the valve into the bypass position reduces the system delay volume
20. available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 1200 Series Autosamplers User Manual 143 9 Appendix Safety Symbols Table 33 Safety Symbols Symbol Description A f A The apparatus is marked wi
21. by the complete Autosampler s flow path volume e g 270ul for G1367B Here the mobile phase is directed to the column without passing sample loop needle and needle seat capillary This can help to have faster cycle times especially if low flow rates have to be used like it is mandatory in narrow bore and micro bore HPLC Having the valve in bypass position can increase the carry over in the system The injection cycle times also depend on the injection volume In identically standard condition injecting 100 ul instead of 1 ul increase the injection time by approximately 8 sec In this case and if the viscosity of the sample allows it the draw and eject speed of the injection system has to be increased 1200 Series Autosamplers User Manual 75 5 Optimizing Performance Precise Injection Volume 76 Injection Volumes Less Than 2 pl When the injection valve switches to the BYPASS position the mobile phase in the sample loop is depressurized When the syringe begins drawing sample the mobile phase is further subjected to decreasing pressure If the mobile phase is not adequately degassed small gas bubbles may form in the sample loop during the injection sequence When using injection volumes lt 2 ul these gas bubbles may affect the injection volume precision For best injection volume precision with injection volumes lt 2 ul use of the Agilent 1200 Series degasser is recommended to ensure the mobile phase is adequately degass
22. directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the autosampler is calculated from the needle pusher position 10 Remove the silicon safety tube from the needle 11 Replace the plate tray in the tray base Re install the side door and close the front door 1200 Series Autosamplers User Manual 123 7 Maintenance 12 In the user interface close the Change Loop Capillary function and exit themaintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Loop Capillary function can be found under the Tools icon 124 1200 Series Autosamplers User Manual Maintenance 7 Peristaltic Pump When Tools required Parts required Tubing blocked or broken sand paper Partnumber Description 1 5065 4445 Peristaltic pump with Pharmed tubing The peristaltic pump is a replaceable unit The tubing inside the pump is not replaceable Aa O N Remove the corrugated leak tubing Press the two clips on the front of the peristaltic pump Pull the pump forward off the motor shaft Disconnect the tubing leading to the wash port and the tubing coming from the solvent bottle Connect the wash port tubing to the upper tubing of the new pump use sandpaper to get a good grip on the tubing Connect the tubing coming from the solvent bottle to the lower tubing of the new pump 7 Push the pump onto the motor shaft
23. front door 6 Position 7 Ports 1200 Series CAN valve Assay Reorder G1156 60001 number for repairs only Stator for G1156A valve 0101 1410 1 Groove Rotor seal for G1156A valve 0101 1411 Wellplate Autosampler Purge Accessory kit not orderable G1373 68705 separately includes items 6 22 2x Solvent bottle clear glass 11 9301 1420 2x Bottle head assembly G1311 60003 Tubing Set 100 cm 2x for solvent connection from degasser G1373 67300 to the purge valve 2x PEEK Adapter for tubing from degasser to valve port 0100 1847 Blanking Nut SST 01080 83202 Flexible Capillary 0 25 mm ID length 320 mm 5065 9980 1 16 front ferrules for SST capillaries 1 16 back ferrules 5065 4454 for SST capillaries Fitting Screw long pack of 10 3 groove Rotor seal for max 600 bar G1367D WPS injection 0101 1409 valve purge kit setup Restriction capillary for injection valve waste outlet G1373 87300 CAN cable 1 m long for 1200 Series module to module CAN 5181 1519 connections DC CAN cable to supply power to the G1156A valve frome g 5181 1533 the G1367B WP ALS Rheotool socket wrench 8710 2391 1200 Series Autosamplers User Manual 137 8 Parts and Materials for Maintenance Table 31 Injector Purge Kit Parts for G1367B Autosamplers Description Part Number 9 64 Hex key driver 8710 2394 Priming Syringe for solvent inlet tubing reorder number 5062 8534 pack of 10 Syringe Adapter for priming syringe 9301 1337 C
24. injector program When the automated needle wash is used the needle is moved into the wash port after the sample is drawn By washing the needle after drawing a sample the sample is removed from the outer surface of the needle immediately As the flush port is automatically refilled with fresh wash solvent this option should be used routinely Using wash vials is usually not necessary but available for special applications Using a wash vial If a wash vial is used it should be considered not to cap the vial Otherwise small amounts of sample remain on the surface of the septum which may be carried on the needle to the next sample Using the Flush Port During the injection process when the sample is in the loop and when the valve still is in Bypass the outside of the needle can be washed in a flush port located behind the injection port on the sampling unit During the wash cycle as soon the needle is in the flush port a peristaltic pump fills the flush port with fresh solvent during a defined time The volume of the flush port is about 680 ul and the pump delivers 6 ml min Setting the wash time to 10 seconds is sufficient to refill 2 times the flush port In most cases this is enough to clean the needle outside Additionally after the needle left the flush port the flush pump keeps running runs for 6 sec to ensure refill with fresh solvent At the end of this flush process if injection with needle wash is selected the needle r
25. low carry over for very sensitive analysis The bottle containing the mobile phase for the wash procedure will be located in the solvent bottle cabinet Produced waste during this operation is channeled safely away through a waste drain The six port only 5 ports are used injection valve unit is driven by a high speed hybrid stepper motor During the sampling sequence the valve unit bypasses the autosampler and connects flow from the pump to the column directly During injection and analysis the valve unit directs the flow through 1200 Series Autosamplers User Manual 9 1 10 Introduction the autosampler which ensures that all of the sample is injected onto the column and that the metering unit and needle are always free from sample residue before the next sampling sequence begins All the injection valves have different stator heads and different rotor seals The volume of each valve is different Control of the vial plate temperature in the thermostatted autosampler is achieved using an additional Agilent 1200 Series module the Agilent 1200 Series thermostat for ALS FC Spotter The thermostat contains Peltier controlled heat exchangers A fan draws air from the area above the sample vial tray of the autosampler It is then blown through the fins of the cooling heating module There it is cooled or heated according the temperature setting The thermostatted air enters the autosampler through a recess underneath the special design
26. operation to avoid discrimination of samples 1200 Series Autosamplers User Manual Optimizing Performance 5 e Notice backpressure of a new column an increase of 10 over time may cause an 10 fold increase in carry over due to the column e Blank vials can be used at least 30 injections Specific recommendations if bypass operation is performed Bypass operation can severely impact carry over performance due to the fact that during gradient operation the sample path is not flushed with organic mobile phase This may cause sample discrimination and or adsorption of especially lipophilic components in loop needle and seat flow path The term bypass operation in this context describes all cases where the Autosampler is switched to the Bypass Mode so that the exposure of the Autosampler s internal flow path parts to the solvent flow coming from the pump may become too short This may be the case e when overlapped Injection with option when sample is flushed out is selected e when the delay volume is minimized by using Automated Delay Volume Reduction This mode is not recommended as there may arise two sources for carry over The outlet groove of the injection valve may be contaminated with sample This is the minor issue and can be resolved by performing steps for cleaning the injection valve by method or by injector program The much more problematic issue is that there may remain portions of sample in the sample
27. performance All Agilent 1200 series High Performance Autosampler are considering low carry over Nevertheless the Agilent 1200 series High Performance Autosampler SL is best performing regarding carry over General recommendations for lowest carry over Issues in setting up the experiments e Use a flush solvent which dissolves the sample well e g aqueous organic acidic for basic samples set needle wash time to at least 10 sec Use a sample solvent which is compatible with sample and mobile phase Organic sample solvents e g DMSO injected into aqueous mobile phase often cause samples to partially deposit on surfaces causing high carry over Chlorhexidine for example dissolved as a free base in methanol and injected into an acidic aqueous mobile phase shows increased carryover than if it is dissolved in 0 1 TFA Since it dissolves slowly but well in acidic aqueous solvents it partially deposits on surfaces during the injection cycle Take care at loop capillary change Push loop capillary forward when tightening the fitting to the needle ensuring gapless transition from loop to needle A replaced needle seat pair may need 100 200 injections for best carry over results Routinely work Prime flush pump for 30 sec with appropriate solvent previous to the first run after usage outage Ensure that the needle seat is not contaminated For cleaning the needle seat see chapter Cleaning the needle seat Use mainpass
28. until the clips click into place 8 Reinstall the corrugated leak tubing 1200 Series Autosamplers User Manual 125 7 Maintenance Installing Interface Board When For all repairs inside the sampler or for installation of the board Tools required Flat head screwdriver Parts required Description 1 Interface board CAUTION Electrostatic discharge at the interface board The interface board is sensitive to electrostatic discharge gt Always use the ESD strap when handling electronic boards 1 Switch off the autosampler at the main power switch 2 Disconnect all cables from the existing interface board Then loosen the interface board holding screws and slide the board out of its holding rails 3 Identify the interface board slot cover Loosen the two holding screws and remove the cover 4 Carefully insert the new interface board into the holding rails and push the board into the slot Make sure the board plugs into the socket correctly 5 Reconnect all cables to the new interface board Switch on the sampler 126 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual e 8 Parts and Materials for Maintenance Sampler Main Assemblies 128 Vial Trays 131 High Performance Autosampler and SL Version Accessory Kit G1367 68705 133 Micro Well Plate Autosampler Accessory Kit G1377 68705 134 Multi Draw Kit G1313 68711 Only For G1367B 136 G1373A Injector Purge Kit Parts 137 Thermostat
29. use them see LMD Lab Monitor and Diagnostic software documentation 1200 Series Autosamplers User Manual 21 1 Introduction Electrical Connections Electrical Connections CAN cable to previous module Mialn mber output EEE o a f _ ontrol of Thermostat game L 2 Jez Lamy of ed m Teo 0 mo oo ie casa m o ise Remote RS232C DC Out CAN bus CAN cable to next module Figure 7 Autosampler Electrical Connections 22 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual e 2 Site Requirements and Specifications Site Requirements 24 Power Consideration 24 Power Cords 25 Bench Space 26 Environment 26 Physical Specifications 27 Performance Specifications 28 ee Agilent Technologies 23 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimum performance of the autosampler Power Consideration The autosampler power supply has wide ranging capability see Table 3 on page 27 Consequently there is no voltage selector in the rear of the autosampler There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply The thermostatted autosampler comprises two modules the sampler G1367B D or G1377A and the thermostat G1330B Both modules have a separate power supply and a power plug for the line connections The two modules are connected by a control
30. 2 let s the needle move above the seat and switches the valve to mainpass so that the pump s solvent is directed through the loop and the needle onto the seat The liquid leaves the seat via the drainage for the flush port The offset can be used for getting a kind of blast pipe effect Generally an offset of 0 0mm is a proper value After the flush time here 90sec the valve is switched to bypass Line 3 moves the needle back into the seat and switches valves back to main pass to restore hydraulic flow as it was before the cleaning process The pump s time table can be used if special solvents for cleaning are connected to the pump or if the flushing flow should be adjusted Here s an illustrating example Time B Flow Max Press 1 0 00 100 0 2 0 10 0 500 3 0 11 3 000 4 0 70 100 0 3 000 5 0 71 0 0 0 500 Along with upper injector program this time table uses solvent B for cleaning the seat with an even higher flow rate than the perhaps limiting 0 5ml min for column flow To ensure that the flow rate isn t applied to the column and that column doesn t come in contact with flush solvent in this case solvent B choose a considerably long time in this case 90sec for flushing the seat in the injector program Manual procedure If the automatic procedure doesn t succeed there is the semi automatic seat back flushing Preparation e Move the needle to home position e Set pump flow to zero 1200 Series Autosamplers U
31. 4 or higher and the ChemStation revision to A 08 04 or higher Y Perform an auto alignment without any plates in place V Change the tray G2258 60011 see service note G1367 007 1200 Series Autosamplers User Manual 95 6 Troubleshooting and Diagnostics Needle Centering Over the Vial or the Well 96 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual e 7 Maintenance Introduction to Maintenance and Repair 98 Simple Repairs Maintenance 98 Exchanging Internal Parts Repairs 98 Warnings and Cautions 98 Using the ESD Strap 99 Cleaning the module 100 Overview of Main Repair Procedures 101 Maintenance Functions 102 Early Maintenance Feedback EMF 103 EMF Counters 103 Using the EMF Counters 104 Setting the EMF Limits 104 Maintenance Procedures 105 Removing the Needle Assembly 106 Installing the Needle Assembly 108 Removing the Needle Carrier Assembly 110 Installing the Needle Carrier Assembly 111 Exchange the Needle Seat Assembly G1367 87101 on the G1367B Samplers 112 Exchange the Needle Seat G1367 87105 on the G1367D Exchange the Needle Seat G1377 87101 onthe G1377A 113 Exchange the Seat Capillary on the G1377A and G1367D 115 Stator Face 116 Rotor Seal 117 Metering Seal and Plunger 119 Removing the Loop Capillary 121 Installing the Loop Capillary 123 Peristaltic Pump 125 Installing Interface Board 126 ott Agilent Technologies 97 7 Maintenance Introduction to Maintenanc
32. 67 87302 Needle Seat assy for G1377A without capillary G1377 87101 Seat cap 0 10 mm ID for G1377 87101 Needle Seat G1375 87317 Seat cap 0 075 mm for G1377 87101 Needle Seat G1375 87316 Seat cap 0 05 mml for G1377 87101 Needle Seat G1375 87300 8 Plate Tray base G2258 60011 9 Needle assy for G1367B D G1367 87202 Needle assy for G1377A G1377 87201 10 Needle Carrier assembly G1367 60010 11 Power supply assembly not visible 0950 2528 12 Autosampler Main Board MTP G1367 66520 Exchange Assembly MTP board G1367 69520 13 Ribbon Cable from ST to MTP G1364 81601 Ribbon Cable from SLS to MTP not visible G1367 81600 14 Loop capillary for G1367B G1367 60007 Loop capillary for G1367D G1377 87310 Loop capillary for G1377A 8pl injection volume G1375 87315 Loop capillary for G1377A 40ul injection volume G1377 87300 1200 Series Autosamplers User Manual 129 8 Parts and Materials for Maintenance Table 24 Autosampler Main Assemblies Item Description Part Number 15 Illumination assembly for sampler Sampler TCC cap 380 mm 0 17 mm id for G1367 68A Sampler TCC cap 500 mm 0 05 mm id for G1377 78A Fan not visible Fan exhaust not visible BCD board not visible G1367 60040 01090 87306 G1375 87304 3160 1017 3160 4097 G1351 68701 130 1200 Series Autosamplers User Manual Parts and Materials for Maintenance 8 Vial Trays Vial Trays Table 25 Autosampler Vial Trays and Tray
33. 8710 2391 8710 2392 8710 2394 8710 2438 9300 1408 G1329 43200 G1367 87304 G1367 60006 G1377 44900 Table8 Micro well plate Autosampler Accessory Kit Contents G1377 68705 Description Quantity Part Number 96 well plate 0 5 ml PP pack of 10 1 5042 1386 Tubing assembly 1 5063 6527 1200 Series Autosamplers User Manual 35 3 Installing the Autosampler Table8 Micro well plate Autosampler Accessory Kit Contents G1377 68705 Description Quantity Part Number Filter kit 1 5064 8240 CAN cable 1m 1 5181 1519 Vials screw cap 100 pk 1 5182 0716 Blue screw caps 100 pk 1 5182 0717 Hex key 9 64 inch for injection valve screws 1 8710 0060 Wrenches 1 4 5 16 inch 2 8710 0510 Wrench 4 0 mm open end 1 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 2391 Hex key 4 0 mm 15 cm long T handle 1 8710 2392 Hex key 9 64 inch 15 cm long T handle 1 8710 2394 Hex key 2 5 mm 15 cm long straight handle 1 8710 2412 Hex key 2 0 mm 1 8710 2438 ESD wrist strap 1 9300 1408 Torque adapter 1 G1315 45003 Air channel adapter 1 G1329 43200 Capillary sampler column 500 mm 0 05 mm ID 1 G1375 87304 40 pl Loop capillary 1 G1377 87300 WPS leak kit 1 G1367 60006 Seat capillary 150 mm 0 075 mm ID 1 G1367 87316 Tool for micro seat capillary 1 G1377 44900 36 1200 Series Autosamplers User Manual Installing the Autosampler 3 Optimizing the Stack Configuration If your autosampler is part of a system you can ensure optimum perfo
34. 87101 Needle Seat without capillary for G1377A Optional G1375 87317 Seat Capillary 150 mm 0 10 mm ID for G1377 87101 Needle Seat 1 G1375 87316 Optional Seat Capillary 150 mm 0 075 mm ID for G1377 87101 Needle Seat 1 G1375 87300 Optional Seat Capillary 150 mm 0 05 mm ID for G1377 87101 Needle Seat Ht 1 In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Software the change needle seat function can be found under the Tools icon 2 Open the front door and remove the side door Remove the plate tray from the tray base A Disconnect the seat capillary from the needle seat with the 4 mm open wrench Use the flat head screwdriver to remove the needle seat Insert the new needle seat Press it firmly in position Connect the seat capillary to the needle seat with the 4 mm open wrench on ol Replace the plate tray in the tray base Re install the side door and close the front door 9 In the user interface close the Change Needle Seat function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the change needle seat function can be found under the Tools icon 1200 Series Autosamplers User Manual 113 7 Maintenance Maintenance Procedures NOTE The seat capillary can be exchanged alone if the needle seat is not damaged 114 1200 Series Autosamplers User Manual
35. 87315 Loop capillary injection volume up to 8 pl for the G1377A G1377 87300 Loop capillary injection volume up to 40 ul for the G1377A Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful when you remove the loop capillary gt Use the silicon safety tube for the needle If the loop capillary is not broken and no solvent has leaked into the loop capillary tube the solvent draw up steps using the syringe can be skipped steps 5 6 8 oF WwW N In the user interface start the maintenance mode and select the Change Loop Capillary function In the Agilent Lab Monitor amp Diagnostic Software the Change Loop Capillary function can be found under the Tools icon Open the front door and remove the side door Remove the plate tray from the tray base Push the silicon safety tube over the needle Remove the corrugated loop capillary waste tube and introduce the small tubing from the leak kit into the loop capillary protection tube 6 Draw up the liquid with the syringe 7 Unlock the needle tighter lock system 8 Draw up the rest of the solvent from the loop capillary protection tube 1200 Series Autosamplers User Manual 121 7 122 Maintenance 9 Loosen the loop capillary fitting on the analytical head side 10 Pinch the holder clamp pull back and remove the needle assembly with the loop capillary from the needle carrier 11 Attach the 5 16 inch wrenc
36. Acetonitrile 85 15 Column Flow rate 13 pl min Injection volume 1 pl caffeine 25 ng caffeine 1 ul water to test carryover Outside wash of needle before injection 20 sec with water using flush port 1200 Series Autosamplers User Manual 31 2 Site Requirements and Specifications Performance Specifications 32 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual e 3 Installing the Autosampler Unpacking the Sampler 34 Damaged Packaging 34 Delivery Checklist 34 Accessory Kits 34 Optimizing the Stack Configuration 37 Installing the Autosampler 42 Installing a Thermostatted Autosampler 45 Flow Connections tothe Sampler 49 Installing the Sample Tray 51 Transporting the Sampler 53 ee Agilent Technologies 33 3 Installing the Autosampler Unpacking the Sampler 34 If you need to ship the autosampler at a later date always use the shipping protection foam parts see Transporting the Sampler on page 53 Damaged Packaging Upon receipt of your autosampler inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for completeness and the autosampler has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carrier s inspection Delivery Checklist Ensure all parts and mater
37. Agilent 1200 Series High Performance Autosamplers amp Micro Well Plate Autosampler User Manual ott Agilent Technologies Notices Agilent Technologies Inc 2006 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61367 90011 Edition 02 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that
38. Analytical head Technical Data Standard 100 pl High Pressure 40pl Micro 40 pl G1367 60003 G1377 60023 G1377 60013 Number of steps 15000 15000 60000 Volume resolution 14 nl motor step 5 6 nl motor step 1 4 nl motor step Maximum stroke 100 ul 40 pl 40 pl Pressure limit 400 bars 600 bars 400 bars Plunger material Sapphire Sapphire Sapphire Injection Valve A high pressure 6 port 2 position valve to direct streams of mobile phase and sample to different directions e g via loop to column or directly to column The two position 6 port injection valve is driven by a stepper motor Only five of the six ports are used port 3 is not used A lever slider mechanism transfers the movement of the stepper motor to the injection valve Two microswitches monitor switching of the valve bypass and mainpass end positions The injection valve has a ceramic stator Vespel rotor seal Tefzel 1200 Series Autosamplers User Manual Introduction 1 seal available and stainless steel head Three screws hold the head and internal components in place No valve adjustments are required after replacing internal components Table 2 Injection Valve Technical Data Standard 0101 0921 Micro 0101 1050 High pressure 0101 1422 Motor type 4V 1 2 A stepper motor 4 V 1 2 A stepper motor 4 V 1 2 A stepper motor Seal material Vespel or Tefzel Vespel PEEK Stator material Ceramic PEEK Head coated SST Ultralife Number of ports 6 6 6
39. Base Item Description Part Number 1 Tray for 2 plates 10 x 2 ml vials G2258 60011 2 Screws for springs 0515 0866 3 Spring G1313 09101 4 Spring stud 0570 1574 5 Tray base includes items 4 5 6 G1329 60000 6 Adapter air channel G1329 43200 Plug channel not shown G1367 47200 Figure 25 Vial trays and Tray Base 1200 Series Autosamplers User Manual 131 8 Parts and Materials for Maintenance Table 26 Recommended plates and closing mat Description Rows Columns Plate height Volume pl Part Number Package 384 Agilent 16 24 14 4 80 5042 1388 30 384Corning 16 24 14 4 80 No Agilent PN 384Nunc 16 24 14 4 80 No Agilent PN 96Agilent 8 12 14 3 400 5042 1386 10 5042 1385 120 96Agilent conical 8 12 17 3 150 5042 8502 25 96CappedAgilent 8 12 47 1 300 5065 4402 1 96Corning 8 12 14 3 300 No Agilent PN 96CorningV 8 12 14 3 300 No Agilent PN 96DeepAgilent31mm 8 12 31 5 1000 5042 6454 50 96DeepNunc31mm 8 12 31 5 1000 No Agilent PN 96DeepRitter41mm 8 12 41 2 800 No Agilent PN 96Greiner 8 12 14 3 300 No Agilent PN 96GreinerV 8 12 14 3 250 No Agilent PN 96Nunc 8 12 14 3 400 No Agilent PN Closing mat for all 96 8 12 5042 1389 50 Agilent plates Table 27 Recommended Vial plates Description Part Number Vial plate for 54x2ml vials 6 pk Vial plate for 15x6ml vials 1 pk Vial plate for 27 Eppendorf tubes 1 pk G2255 68700 5022 6539 5022 6538 132 1200 Series Autosamplers User Manual Parts and Materials for Main
40. Head 5063 6586 Metering Plunger for the G1367 60003 100 ul Analytical Head 5022 2175 Metering Seal pack of 1 for the G1377 60013 40 pl Analytical Head 5064 8293 Metering Plunger for the G1377 60013 40 pl Analytical Head In the user interface start the maintenance mode and select the Change Piston function In the Agilent Lab Monitor amp Diagnostic Software the Change Piston function can be found under the Tools icon Open the front door Remove the corrugated leak tubing 4 Remove the two capillaries from the analytical head Use a 1 4 inch wrench oo N OOQ if you have a SST capillary or a 4 mm wrench if you have a fused silica capillary Unscrew alternately the two fixing screws with the 4 mm hex key and remove them Pull the analytical head away from the sampling unit Remove the two fixing screws from the base of the analytical head Remove the head body Using the piston carefully remove the metering seal Clean the chamber and ensure all particular matter is removed Installing the Metering Seal When poor injection volume reproducibility When leaking metering device 1200 Series Autosamplers User Manual 119 7 Maintenance Tools required Parts required 120 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4 mm open end wrench 8710 1534 supplied in accessory kit 4 mm 15 cm long T handle hex key 8710 2392 supplied in accessory kit Small flat head screwdriver Partnumber Desc
41. Injector program capability is offered with the standard instrument Active Needle Wash The active needle wash mode allows also the flushing of the outer surface of the needle This results in an additional decrease of sample carry over Duration of the procedure is setable Overlap Injection Cycle Overlapped injection is the mode where the autosampler runs the injector program for the next analysis during the current analysis without injecting After the sample has reached the column the valve is switched back to bypass and the next injection cycle starts but waits with switching to main pass until the actual run is finished This mode allows it to increase the sample throughput Low Delay Volume Mode This mode is especially interesting for gradient elution with small bore or capillary columns The injection valve is switched back to bypass after the sample is eluted beyond the injection valve port 6 This decreases the delay volume because the gradient needs not to pass the metering device and the loop capillary 1200 Series Autosamplers User Manual Introduction 1 Early Maintenance Feedback EMF The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled For details on EMF counters and how to
42. N 384Nunc 16 24 14 4 80 No Agilent PN 96Agilent 8 12 14 3 400 5042 1386 10 5042 1385 120 96Agilent conical 8 12 17 3 150 5042 8502 25 96CappedAgilent 8 12 47 1 300 5065 4402 1 96Corning 8 12 14 3 300 No Agilent PN 96CorningV 8 12 14 3 300 No Agilent PN 96DeepAgilent31mm 8 12 31 5 1000 5042 6454 50 1200 Series Autosamplers User Manual 57 4 Using the Autosampler Table 10 Recommended plates and closing mat Description Rows Columns Plate height Volume pl Part Number Package 96DeepNunc31mm 8 12 31 5 1000 No Agilent PN 96DeepRitter41mm 8 12 41 2 800 No Agilent PN 96Greiner 8 12 14 3 300 No Agilent PN 96GreinerV 8 12 14 3 250 No Agilent PN 96Nunc 8 12 14 3 400 No Agilent PN Closing mat for all 96 8 12 5042 1389 50 Agilent plates Table 11 Recommended Vial plates Description Part Number e Vial plate for 54 x 2 ml vials 6 pk G2255 68700 e Vial plate for 15 x 6 ml vials 1 pk 5022 6539 e Vial Plate for 27 Eppendorf tubes 5022 6538 58 1200 Series Autosamplers User Manual Using the Autosampler 4 List of Recommended Vials and Caps Table 12 Crimp Top Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5181 3375 5183 4491 Clear glass 2 5182 0543 5183 4492 5183 4494 write on spot Amber glass 2 5182 3376 5183 4493 5183 4495 write on spot Table 13 SnapTop Vials Description Volume ml 100 Pack 1000 Pack 100 Pack silanized Clear glass 2 5182 0544
43. Switching time lt 150 ms lt 150 ms lt 150 ms Needle Flush Station A needle flush station to wash the outer surface of the injection needle and a peristaltic pump to deliver fresh solvent to the wash station The reservoir for the solvent is located in the solvent cabinet the waste is channeled by a separate flex tube to a waste bottle Needle Lock A needle lock is used to support the needle carrier in its function making a firm seal of the needle in its seat The needle lock arm is driven by a stepper motor connected to the spindle assembly by a toothed belt 1200 Series Autosamplers User Manual 17 1 Introduction Needle Sample Transport Assembly Needle Sample Transport Assembly a 3 y fo J C A qn g Needle assembly Reflective light Ia switches X Figure 6 Needle Sample Transport Assembly The needle sample transport is a multifunctional module capable of moving the needle into various positions such as different wells in two different plates different vials needle wash position and the needle seat position The active movable axes are the X axis the Z axis and the theta axis the 18 1200 Series Autosamplers User Manual Introduction 1 vial plate pusher is an additional passive axis All axes are stepper motor driven and encoder controlled in order to have tight feedback for the axes position The theta and Z axes have spring loaded belt tensioner Reflectiv
44. and components Electronic boards and components are sensitive to electrostatic discharge ESD gt In order to prevent damage always use an ESD protection when handling electronic boards and components Using the ESD Strap 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 23 Using the ESD Strap 1200 Series Autosamplers User Manual 99 7 Maintenance Cleaning the module The module case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and mild detergent Do not use an excessively damp cloth as liquid may drip into the module WARNING Liquid shall not touch the module electronics It could cause shock hazard and it could damage the module gt Do not let liquid drip into the module 100 1200 Series Autosamplers User Manual Maintenance 7 Overview of Main Repair Procedures Overview of Main Repair Procedures MTP board Transport assembly Illumination Kit Loop capillary SLS board Power supply Analytical head Needle carrier Peristaltic pump Needle Needle seat Injection valve 1200 Series Autosamplers User Manual 101 7 Maintenance Maintenance Functions Some
45. ation for Lowest Carry Over Carry over CO on 1100 1200 Agilent system is not only a topic for injection systems but may have multiple sources Hardware related sample loop needle outside needle inside needle seat seat capillary injection valve flush time wash vials fittings column carry over depends on frit design material blockage surface activity of frits capillaries Chemistry Physics related suitable sample solvent has to be compatible with mobile phase suitable wash solvent suitable mobile phase column packing material e g interaction of basic sample with silanols of stationary phase The autosampler continuous flow through design ensures that sample loop needle inside seat capillary and the mainpass of the injection valve is always in the flow line These parts are continuously flushed during an isocratic and also during a gradient analysis The residual amount of sample remaining on the outside of the needle after injection may contribute to carry over in some instances When using small injection volumes or when injecting samples of 1200 Series Autosamplers User Manual 67 5 68 Optimizing Performance low concentration immediately after samples of high concentration carry over may become noticeable Cleaning the needle in the flush port or using the automated needle wash enables the carry over to be minimized and prevents also contamination of the needle seat Module dependant carry over
46. b Monitor amp Diagnostic Software 84 The Agilent Lab Monitor amp Diagnostic Software LMD is an application independent tool that provides diagnostic capabilities for all Agilent 1200 Series HPLC modules It includes tests calibrations and tools such as injector steps and maintenance positions In addition to these features of the LMD Basic edition the Advanced edition of LMD provides Guided Diagnostics which interactively supports users of LC instruments to resolve typical issues Based on chromatographic symptoms Guided Diagnostics helps users to identify the root cause of their problem and leads them through the necessary steps to resolve it LMD also allows users to monitor the status of their LC instruments The Early Maintenance Feedback EMF feature helps to carry out preventive maintenance In addition users can generate a status report for each individual LC instrument The tests and diagnostic features as provided by the Agilent Lab Monitor amp Diagnostic Software may differ from the descriptions in this manual For details refer to the LMD help files This manual provides lists with the names of Error Messages Not Ready messages and other common issues 1200 Series Autosamplers User Manual Troubleshooting and Diagnostics 6 Overview of the Sampler s Indicators and Test Functions Status Indicators The autosampler is provided with two status indicators which indicate the operational state prerun not
47. cable and both are turned on by the sampler module The thermostat power supply has two externally accessible fuses Damaged electronics Disconnecting or reconnecting the sampler to thermostat cable when the power cords are connected to either of the two modules will damage the electronics of the modules gt Make sure the power cords are unplugged before disconnecting or reconnecting the sampler to thermostat cable Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your autosampler to the specified line voltage 24 1200 Series Autosamplers User Manual CAUTION Site Requirements and Specifications 2 Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable Power Cords Your autosampler is delivered with a power cord which matches the wall socket of your particular country or region The plug on the power cord which connects to the rear of the instrument is identical for all types of power cord Electric shock The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit
48. conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION
49. ction Volume 76 Choice of Rotor Seal 79 Choice of Seat Capillary 80 Troubleshooting and Diagnostics 83 Agilent Lab Monitor amp Diagnostic Software 84 Overview of the Sampler s Indicators and Test Functions 85 Status Indicators 87 Maintenance Functions 89 High Performance Autosampler Step Commands 91 Troubleshooting the G1367B D and G1377A Autosampler 94 Needle Centering Over the Vial or the Well 95 Maintenance 97 Introduction to Maintenance and Repair 98 Overview of Main Repair Procedures 101 Maintenance Functions 102 Early Maintenance Feedback EMF 103 Maintenance Procedures 105 Parts and Materials for Maintenance 127 Sampler Main Assemblies 128 VialTrays 131 High Performance Autosampler and SL Version Accessory Kit G1367 68705 133 Micro Well Plate Autosampler Accessory Kit G1377 68705 134 Multi Draw Kit G1313 68711 Only For G1367B 136 G1373A Injector Purge Kit Parts 137 Thermostat for ALS FC Spotter 139 Appendix 141 General Safety Information 142 Lithium Batteries Information 145 The Waste Electrical and Electronic Equipment Directive 146 Radio Interference 147 Sound Emission 148 Solvent Information 149 1200 Series Autosamplers User Manual Contents Agilent Technologies on the Internet 150 1200 Series Autosamplers User Manual Contents 6 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual 1 Introduction Introduction to the 1200 Series Samplers Sampling Seque
50. e Autosampler 3 Air channel adapter gt Figure 14 Installation of Thermostat and Autosampler 11 Re install the tray 12 Ensure the power switch on the front of the sampler is OFF and the power cables are disconnected 13 Connect the cable between the sampler and the thermostat see Figure 15 on page 48 14 Connect the power cables to the power connectors 15 Connect the CAN cable to other Agilent 1200 series modules 16 If a Agilent ChemStation is the controller connect the LAN connection to the LAN interface 17 Connect the APG remote cable optional for non Agilent 1200 Series instruments 18 Ensure the side panel is correctly installed 19 Turn ON power by pushing the button at the lower left hand side of the sampler 20 Close the front door The exhaust fan will turn ON and remove the vapor from the tray compartment After 1 2 minutes the sampler will start tile hardware initialisation process At the end of this process the status LED should be off 1200 Series Autosamplers User Manual 47 3 Installing the Autosampler Installing a Thermostatted Autosampler Sampler Thermostat cable CAN bus AC Power Figure 15 Connection at the rear of thermostatted Autosampler NOTE The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The sampler is turned OFF when the line power switch is protruding and the green light is OFF 48 1200 Series
51. e and Repair Simple Repairs Maintenance The autosampler is designed for easy repair The most frequent repairs such as changing a needle assembly can be done from the front of the instrument with the instrument in place in the system stack These repairs are described in Maintenance Procedures on page 105 Exchanging Internal Parts Repairs Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the autosampler from the stack removing the covers and disassembling the autosampler Warnings and Cautions ime Repair work at the autosampler can lead to personal injuries e g shock hazard when the autosampler cover is opened and the instrument is connected to power gt Remove the power cable from the instrument before opening the autosampler cover gt Do not connect the power cable to the autosampler while the covers are removed 98 1200 Series Autosamplers User Manual Maintenance 7 WARNING Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary or tube fittings solvents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used CAUTION Electrostatic discharge at electronic boards
52. e arm to the upper position and Switches the injection valve to the mainpass position Troubleshooting If the autosampler is unable to perform a specific step due to a hardware failure an error message is generated You can use the step commands to perform an injection sequence and observe how the autosampler responds to each command Table 22 on page 92 summarizes the step commands and lists the error messages and probable causes associated with each possible failure Table 22 Step Failures Step Function Probable Failure Modes Valve Bypass Valve not connected Defective injection valve Plunger Home Defective or dirty sensor on the sampling unit flex board Defective metering drive motor Needle Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor Draw Sum of all draw volumes exceeds 100 pl or 40 pl Defective metering drive motor Needle Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle drive motor 1200 Series Autosamplers User Manual Troubleshooting and Diagnostics 6 Table 22 Step Failures Step Function Probable Failure Modes Valve Mainpass Valve not connected Defective injection valve Needle Up Mainpass Blockage in the sample loop or needle no solvent flow Defective or dirty sensor on the sampling unit flex board Sticking needle arm assembly Defective needle dr
53. e light switches detect the presence and type of different trays The X slide carries the antenna and electronics of a RF sensor This device has multiple functions It allows to read and write information from a tag located in the new tray It allows to increase the number of different trays e It allows to read the revision and other data tags of the needle sample transport assembly and sampling unit Complex flex boards make the electrical connection to the various motors sensors and the MTP board The needle carrier has an integrated plate vial pusher with an additional linear encoder to sense vials and the presence of plates The needle and the loop capillary are user exchangeable The back of the needle sample transport assembly has a cover to protect the electronics from potential solvent vapor 1200 Series Autosamplers User Manual 19 1 Introduction Advanced Operating Modes 20 Multi Draw Mode Optional The multi draw mode provides injection volumes up to 1500 ul In this case a capillary which holds the additional volume is assembled between seat and valve Then the aspirated sample is pushed into the enlarged seat capillary before repetitive aspiration starts After the last aspiration took place the injection valve switches and the mobile phase transports sample towards column Injector Program A sequence of all available single sampling steps can be tailored to customer needs for special applications
54. e mobile phase conditions PEEK Seal With the High Performance SL Autosampler a PEEK rotorseal is used This warrants a leak tight system at high pressures and allows the usage of solvents ranging from pH 2 3 to 12 The PEEK material may show a reduced lifetime if used with following solvents e Methylene chloride e DMSO e THF e High concentrations of sulfuric acid e High concentrations of nitric acid 1200 Series Autosamplers User Manual 79 5 Optimizing Performance Choice of Seat Capillary 80 Different models of seat capillaries are available for the high performance autosampler the SL version and the micro well plate autosampler For the high performance autosampler The needle seat assembly includes the needle seat and the seat capillary The part number for this assembly is G1367 87101 For the high performance autosampler SL The needle seat assembly is made up of two parts Needle seat G1367 87105 Seat capillary G1367 87303 0 12 mm 150 mm G1367 87302 0 17 mm 150 mm The capillary pre installed in the high performance autosampler SL is the G1367 87302 For the micro well plate sampler The needle seat assembly is made up of two parts 1200 Series Autosamplers User Manual Optimizing Performance 5 Needle seat G1377 87101 Seat capillary G1375 87317 100 um 150 mm G1375 87316 75 um 150 mm G1375 87300 50 um 150 mm part number G1375 87317 100 pm is the capillary preinstalled
55. e stator screws from the stator head with the 9 64 inch wrench 4 Remove the stator head the stator face and the stator ring 5 Remove the rotor seal and isolation seal if required 6 Install the new rotor seal and isolation seal if required The metal spring inside the isolation seal must face toward the valve body In other words the metal spring should not be visible when the isolation seal is installed 1200 Series Autosamplers User Manual 117 7 Maintenance 7 Reinstall the stator ring 8 Place the stator face on the stator head The pins on the stator face must engage in the holes on the stator head 9 Install this stator head face assy on the injection valve Tighten the screws alternately with the 9 64 inch wrench until the stator head is secure 10 Reconnect all the capillaries to the injection valve ports with the 1 4 inch wrench 11 Close the front cover 118 1200 Series Autosamplers User Manual Maintenance 7 Metering Seal and Plunger When Tools required Parts required When Removing the Metering Seal When poor injection volume reproducibility When leaking metering device 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4 mm open end wrench 8710 1534 supplied in accessory kit 4 mm 15 cm long T handle hex key 8710 2392 supplied in accessory kit Small flat head screwdriver Partnumber Description 5063 6589 Metering Seal pack of 2 for the G1367 60003 100 pl Analytical
56. e the Needle Seat G1377 87101 on the G1377A on page 113 to finish the installation Choose the seat capillary diameter as function of the column and the application you run on the system See Choice of Seat Capillary on page 80 1200 Series Autosamplers User Manual 115 7 Maintenance Stator Face When Tools required Parts required CAUTION When poor injection volume reproducibility When leaking injection valve 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 9 64 inch 15 cm long T handle hex key 8710 2394 supplied in accessory kit Partnumber Description 1 0100 1851 Stator Face Damage of the stator face The stator face is held in place by the stator head When removing the stator head the stator face can be damaged gt When removing the stator head ensure the stator face does not fall out of the valve 116 This procedure is only for the injection valve on the G1367B samplers The injection valve on the G1367D and the G1377A samplers have no ceramic stator face 1 Open the front door 2 Remove all capillaries from the injection valve ports with the 1 4 inch wrench 3 Unscrew and remove the three stator screws from the stator head with the 9 64 inch wrench 4 Remove the stator head and stator face Place the new stator face 0100 1851 on the stator head The pins on the stator face must engage in the holes on the stator head 6 Install this stator head face as
57. ed Also using the automated needle wash see Using the Automated Needle Wash on page 70 between injections reduces carry over to a minimum improving injection volume precision further Draw and Eject Speed Draw Speed The speed at which the metering unit draws sample out of the vial may have an influence on the injection volume precision when using viscous samples If the draw speed is too high air bubbles may form in the sample plug affecting precision The default draw speed is suitable for the majority of applications however when using viscous samples set the draw speed to lower speed for optimum results A DRAW statement in an injector program also uses the draw speed setting which is configured for the autosampler 1200 Series Autosamplers User Manual Optimizing Performance 5 Eject Speed The default draw speed is suitable for the majority of applications When using large injection volumes setting the eject speed to a higher value speeds up the injection cycle by shortening the time the metering unit requires to eject solvent at the beginning of the injection cycle when the plunger returns to the home position An EJECT statement in an injector program also uses the eject speed setting which is configured for the autosampler A faster eject speed shortens the time required to run the injector program When using viscous samples a high eject speed should be avoided Table 19 Draw and eject speed
58. ed sample tray The air is then distributed evenly through the sample tray ensuring effective temperature control regardless of how many vials are in the tray In cooling mode condensation is generated on the cooled side of the Peltier elements This condensed water is safely guided into a waste bottle for condensed water 1200 Series Autosamplers User Manual Introduction 1 Sampling Sequence Sampling Sequence MTP board Sample transport Loop capillary Illumination Kit Sampling unit Power supply Analytical head Peristaltic pump Vial tray Switching valve Needle a S NP Needle seat Figure 1 Overview of the autosampler The movements of the autosampler components during the sampling sequence are monitored continuously by the autosampler processor The processor defines specific time windows and mechanical ranges for each movement If a specific step of the sampling sequence is not completed successfully an error message is generated Solvent is bypassed from the autosampler by the injection valve during the sampling sequence The needle moves to the desired sample vial position and is lowered into the sample liquid in the vial to allow the metering device to draw up the desired volume by moving its plunger back a certain distance The needle is then raised again and moved onto the seat to close the sample loop Sample is applied to the column when the injection valve returns to the mainpass position a
59. edge bottom to the center of the first hole A1 G Column offset Distance in mm from the left edge bottom to the center of the first hole A1 H Column shift Offset in mm to Y when the rows Well diameter J Well depth are not straight but staggered Diameter in mm of the well at least 4 mm Distance in mm from the top ofthe upto 45mm plate to the bottom of the well The distances need to be measured with high precision It is recommended to use calipers 1200 Series Autosamplers User Manual 63 4 Using the Autosampler Turn ON and Initialization Steps 64 StatusLED Power LED A successful turn on initialization takes about 3 5 minutes and consists of five steps 1 WPS turn on begins when the main power button is pushed ON Power indicator turns green Front cover latch activates immediately Main fan and exhaust fan turn on immediately Main board self test begins Status indicator tests red green and yellow then goes to yellow This takes about 20 seconds from turn on The status indicator remains yellow until the initialization process is complete The user interface indicates initializing during this period The vapor blowout period begins This lasts for about 2 minutes 5 WPS sample transport and sampling unit initialization begins at the 2 minutes mark from turn on if the front cover is closed If the front cover is open at the 2 minutes mark initialization will start o
60. endorf tube plate Also compatible with the Agilent 1200 Series sample capacity extension for further expansion of the sample capacity Typically lt 30 s using following standard conditions Default draw speed 200 pl min Default eject speed 200 l min Injection volume 5 ul Typically lt 0 01 using the following conditions Column 125 x 4 mm Hypersil ODS 5 pm Mobile phase Water Acetonitrile 80 20 Flow rate 1 ml min Injection volume 1 pl caffeine 1 mg ml 5 pl water to test carryover Outside wash of needle before injection 20 sec with water using flush port 28 1200 Series Autosamplers User Manual Site Requirements and Specifications 2 Table5 Performance specifications Agilent 1200 Series High Performance Autosampler SL Type GLP features Communications Safety features Injection range Precision Pressure range Sample viscosity range Sample capacity Specification Early maintenance feedback EMF electronic records of maintenance and errors Controller area network CAN RS232C APG remote standard optional four external contact closures and BCD vial number output Leak detection and safe leak handling low voltages in maintenance areas error detection and display 0 1 40 pl in 0 1 pl increments Up to 100 pl with extended injection volume hardware modification required Typically lt 0 25 RSD from 5 40 ul Typically lt 0 5 RSD from 2 5 ul Typically lt 0 7 RSD from 1
61. eries 145 saftey class 142 sample trays 56 numbering of vial positions 57 sampling sequence 11 sampling unit 15 seals 79 seat capillary 80 shipping containers 34 shipping 53 site requirements 24 solvent information 149 solvents 149 sound emission 148 specifications 27 28 stack configuration rearview 39 rearview 41 thermostatted autosampler 41 stator 16 status indicator 85 status indicators 87 step functions 86 step commands 91 storage 26 T Tefzel seal 79 temperature 28 26 thermostat 139 theta axis 18 transport assembly 18 transport mechanism 8 transport 53 transporting the autosampler 53 tray combinations 57 trays 56 U unpacking the autosampler 34 V valve capillaries 49 Vespelseal 79 vial contents temperature 28 vial numbering 56 vial tray 53 vial racks 8 vials 8 viscous samples 76 76 Ww weight 26 X X axis 18 Z Z axis 18 1200 Series Autosamplers User Manual Index 1200 Series Autosamplers User Manual 153 www agilent com In This Book This manual contains user information about the Agilent 1200 Series High Performance Autosamplers amp Micro Well Plate Autosampler The manual describes the following e introduction to the sampler e site requirements and specifications e installing and using the sampler e optimizing performance e troubleshooting and diagnostics e maintenance e parts and materials e safety and legal information Agilent Technolog
62. eturns to the injection port the injection valve is switched to the mainpass position and directs the pump flow back through the sample loop 1200 Series Autosamplers User Manual Optimizing Performance 5 For further information on how to reduce carry over see Optimization for Lowest Carry Over on page 67 Recommended Wash Solvents e water e ethanol e methanol e water acid especially for basic compounds e water base espeecially for acidic compounds e water acetonitrile The life time of the tubing in the peristaltic pump is shortened by the usage of organic solvents Cleaning the needle seat If flush port has run out of solvent or the option needle wash hasn t been used for several injections or in case the needle seat has got contaminated the needle seat may be contaminated and carry over is significantly higher than expected For cleaning the needle seat there is an automatic procedure using the mobile phase s solvents If that doesn t work a manual cleaning has to be done the following procedure can be used to clean the needle seat Automatic procedure There s an injector command for flushing the seat So a cleaning method can be set up using an injector program Injector program e INJECT e FLUSH SEAT for 90 0 sec 0 0 mm offset 1200 Series Autosamplers User Manual 71 5 72 Optimizing Performance e VALVE mainpass Line 1 starts the run so the pump s time table is started Line
63. for ALS FC Spotter 139 ot Agilent Technologies 127 Parts and Materials for Maintenance Sampler Main Assemblies Sampler Main Assemblies oO gt A 15 A N i IK L KA an eer a S O FPS gt G hI sm Figure 24 Autosampler Main Assemblies Table 24 Autosampler Main Assemblies Part Number Description Item G1313 81602 Ribbon Cable from SU to MTP G1367 60019 Sample Transport assembly for G1367B D Sample Transport assembly for G1377A G1377 60009 1200 Series Autosamplers User Manual 128 Parts and Materials for Maintenance 8 Table 24 Autosampler Main Assemblies Item Description Part Number 3 Sampling Unit assembly for G1367B G1367 60008 Sampling Unit assembly for G1367D G1367 60028 Sampling Unit assembly for G1377A G1377 60008 The assy comes without injection valve and analytical head 4 SLS board not shown G1367 66505 5 Analytical Head assembly 100 ul for G1367B G1367 60003 Analytical Head assembly 40 ul for G1367D G1377 60023 Analytical Head assembly 40 ul for G1377A G1377 60013 6 Injection Valve assembly for G1367B 0101 0921 Injection Valve assembly for G1367D 0101 1422 Micro Injection valve assembly for G1377A 0101 1050 7 Needle Seat assy for G1367B G1367 87101 Needle Seat assy for G1367D without capillary G1367 87105 Seat cap 0 12 mm for G1377 87104 Needle Seat G1367 87303 Seat cap 0 17 mm for G1377 87104 Needle Seat G13
64. h is designed to carry the weight of all the modules For a complete system including the thermostatted sampler it is recommended to position the modules in two stacks see Figure 8 on page 38 Make sure that in this configuration there is 25 cm 10 inches space on either side of the thermostatted sampler for the circulation of air Environment Your autosampler will work within specifications at ambient temperatures and relative humidity as described in Table 3 on page 27 CAUTION Condensation within the autosampler Condensation will damage the system electronics gt Do not store ship or use your autosampler under conditions where temperature fluctuations could cause condensation within the autosampler gt If your autosampler was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 26 1200 Series Autosamplers User Manual Site Requirements and Specifications 2 Physical Specifications Table 3 Physical Specifications sampler G1367B D G1377A Type Specification Comments Weight 15 5 kg 34 2 Ibs Dimensions height x width x depth 200 x 345 x 440 mm 8 x 13 5 x 17 inches Line voltage 100 240 VAC 10 Wide ranging capability Line frequency 50 or 60 Hz 5 Power consumption apparent power 300 VA Maximum Power consumption active power 200 W Maximum Ambient operating temperature 4 to 55 C 41 to 131 F Ambient non operating temperature
65. h to hold position at the needle assembly Use the 4 mm wrench to loosen the fitting of the loop capillary 12 Pull the loop capillary out from the needle assembly 1200 Series Autosamplers User Manual Maintenance 7 Installing the Loop Capillary When Capillary blocked Capillary broken Tools required Two 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit Parts required Partnumber Description G1367 87300 Loop capillary injection volume up to 100 pl for the G1367B G1377 87310 Loop capillary injection volume up to 40 pl for the G1367D G1375 87315 Loop capillary injection volume up to 8 pl for the G1377A G1377 87300 Loop capillary injection volume up to 40 ul for the G1377A Pull the new loop capillary in the needle assembly 2 Attach the 5 16 inch wrench to hold position at the needle assembly Use the second wrench to tighten the loop capillary fitting 3 Push the loop capillary into the loop capillary protection tube until it comes out on the sampling unit side 4 Reinstall the loop capillary corrugated waste tube over the loop capillary 5 Retighten the loop capillary fitting on the analytical head 6 Pinch the holder clamp and reinsert the needle assembly into the needle carrier 7 Push the black chain into the needle assembly until the stop 8 Lock the needle tighter lock system Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several
66. hemStation CD ROM Rev B 01 03 or higher for the use of N A this kit Installation Note this note N A 138 1200 Series Autosamplers User Manual Parts and Materials for Maintenance 8 Thermostat for ALS FC Spotter Thermostat for ALS FC Spotter Table 32 Thermostat for ALC FC Spotter Item Description Part Number 1 Thermostat exchange assembly G1330 69040 Figure 26 Thermostat for ALS FC Spotter 1200 Series Autosamplers User Manual 139 8 Parts and Materials for Maintenance Thermostat for ALS FC Spotter 140 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual e 9 Appendix General Safety Information 142 Lithium Batteries Information 145 The Waste Electrical and Electronic Equipment Directive 146 Radio Interference 147 Sound Emission 148 Solvent Information 149 Agilent Technologies on the Internet 150 This appendix provides general safety and environmental information ee Agilent Technologies 141 9 Appendix General Safety Information 142 General Safety Information General The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Aligent Technologies assumes no liability for the customer s failure to comply with
67. hesive tape which covers the side and front doors 1200 Series Autosamplers User Manual y OE Cf fF Q 8 9 Installing the Autosampler 3 Open the front door and remove the left side door Remove the transport protection foam Re install the corrugated waste tube in the plastic port Re install the left side door take care of the magnet at the back Place the autosampler in the stack or on the bench in all horizontal position Ensure the power switch at the front of the sampler is OFF Connect the power cable to the power connector at the rear of the sampler 10 Connect the CAN cable to the other Agilent 1200 modules 11 If a Agilent ChemStation is the controller connect the LAN connection to the LAN interface 12 Connect the APG remote cable optional for non Agilent 1200 Series instruments 13 Ensure the side panel is correctly installed 14 Turn ON power by pushing the button at the lower left hand side of the sampler 15 Close the front door The exhaust fan will turn ON and remove the vapor from the tray compartment After 1 2 minutes the sampler will start the hardware initialisation process At the end of this process the status LED should be off 1200 Series Autosamplers User Manual 43 3 Installing the Autosampler Installing the Autosampler CAN cable to previous module Seen ee ee ee g G Relaycontacts gt z k oj gt D i gt ontrol of Thermostat Ge Ge c KAEA Jalg a
68. ials have been delivered with the autosampler For this compare the shipment content with the checklist included in each instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office Accessory Kits Each shipment contents an Accessory kit with the necessary tools to install the system and to have an operating system e The Accessory kit G1367 68705 shown in Table 7 on page 35 is shipped with the G1367B D high performance autosampler and SL version e The Accessory kit G1377 68705 shown in Table 8 on page 35 is shipped with the G1377A micro well plate autosampler 1200 Series Autosamplers User Manual Table 7 High Performance Autosampler and SL version Accessory Kit Contents G1367 68705 Installing the Autosampler 3 Description Capillary sampler column 380 mm 0 17 mm ID 96 well plate 0 5 ml PP pack of 10 Tubing assembly Filter kit CAN cable 1 m Vials screw cap 100 pk Blue screw caps 100 pk Hex key 9 64 inch for injection valve screws Wrench 4mm both ends Rheotool socket wrench 1 4 inch Hex key 4 0 mm 15 cm long T handle Hex key 9 64 inch 15 cm long T handle Hex key 2 0 mm ESD wrist strap Air channel adapter Capillary WPS to column 250 mm 0 17 mm ID WPS leak kit Tool for micro seat capillary Quantity 1 1 1 Part Number 01090 87306 5042 1386 5063 6527 5064 8240 5181 1519 5182 0716 5182 0717 8710 0060 8710 1534
69. ies 2006 2008 Printed in Germany 02 08 G1367 90011 fe Agilent Technologies
70. ignal to recorder 3 to LC ChemStation Figure 11 Recommended Stack Configuration Thermostatted Autosampler Rear View 1200 Series Autosamplers User Manual 41 3 Installing the Autosampler Installing the Autosampler Parts required Preparations Description 1 Sampler Power cord Locate bench space Provide power connections Unpack the sampler Instruments are partially energized when switched off The power supplies still use some power even if the power switch on the front panel is turned off gt To disconnect the thermostatted autosampler from line power unplug the power cord from the autosampler and the ALS thermostat gt Make sure that it is always possible to access the power plug Personal injury To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not attempt to insert or remove a vial or a plate when the needle is positioned CAUTION Defective on arrival problems If there are signs of damage to the autosampler please do not attempt to install the autosampler Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiate appropriate actions 42 1 Install the LAN interface board in the sampler if required 2 Remove the ad
71. in the micro well plate autosamplers upon delivery This capillary is recommended for applications with a 0 3 mm column or higher It provides less plugging of the capillary in general and especially with biological samples For small K this capillary can provide a higher peak width for isocratic analysis part number G1375 87316 75 pm is available as a spare part and is recommended for applications with a 0 3 mm column or smaller This capillary gives full chromatographic performance part number G1375 87300 50 pm is available as a spare part and is recommended for applications with a 0 3 mm column or smaller This capillary gives full chromatographic performance Due to the small diameter this capillary can show some blockage 1200 Series Autosamplers User Manual 81 5 Optimizing Performance Choice of Seat Capillary 82 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual e 6 Troubleshooting and Diagnostics Agilent Lab Monitor amp Diagnostic Software 84 Overview of the Sampler s Indicators and Test Functions 85 Status Indicators 87 Power Supply Indicator 87 Instrument Status Indicator 87 Maintenance Functions 89 Sample Transport Self Alignment 90 High Performance Autosampler Step Commands 91 Troubleshooting 92 Troubleshooting the G1367B D and G1377A Autosampler 94 Needle Centering Over the Vial or the Well 95 ee Agilent Technologies 83 6 Troubleshooting and Diagnostics Agilent La
72. instrument This may damage the instruments electronics gt Make sure that the condensation tube is always above the liquid level in the vessel 1200 Series Autosamplers User Manual 45 3 Installing the Autosampler ime To avoid personal injury keep fingers away from the needle area during autosampler operation gt Do not attempt to insert or remove a vial or a plate when the needle is positioned 1 Place the thermostat on the bench 2 Remove the front cover and route the condensation drain tube to the waste bottle Condensation drain tube Waste bottle j Figure 13 Condensation leak outlet Install the LAN interface board in the sampler if required Remove the adhesive tape which covers the side and front doors Open the front door and remove the left side door Remove the transport protection foam Re install the corrugated waste tube in the plastic port Re install the left side door take care of the magnet at the back oo N OO ol FF amp Place the sampler on top of the thermostat Make sure that the sampler is correctly engaged in the thermostat locks 10 Remove the tray and the plastic cover from the tray base place the air channel adapter into the sampler tray base Make sure the adapter is fully pressed down This assures that the cold airstream from the thermostat is correctly guided to the tray area of the well plate sampler 46 1200 Series Autosamplers User Manual Installing th
73. ive motor Valve not connected Defective injection valve 1200 Series Autosamplers User Manual 93 6 Troubleshooting and Diagnostics Troubleshooting the G1367B D and G1377A Autosampler Gather Information About the Problem e When did the problem start e What was done changed prior to the start of the problem In the Agilent Lab Monitor amp Diagnostic Software the Instrument Status Report generates a report This report includes the Instrument configuration with the instrument serial numbers and the firmware revisions the instrument error history the EMF editor the result of the guided diagnostic and the method parameter optional 94 1200 Series Autosamplers User Manual Troubleshooting and Diagnostics 6 Needle Centering Over the Vial or the Well The positioning of the needle is very precise You have to take no action if the needle hits in the safe area metal border Septum Safe area to hit Figure 22 Vial cap If the diameter for the safe area is approximately 7mm smaller than the diameter of the septum no corrective action is necessary Action to Take if the Needle Does Not Hit in the Safe Area V Check if the right vials or plates are used see or see List of Recommended Vials and Caps on page 59 V Make sure the needle is correctly installed It should be pushed into the needle carrier as far forward as possible and centered in the vial pusher V Upgrade the firmware revision to A 04 1
74. le over the needle Aa O N 5 Pinch the holder clamp and reinsert the needle assembly into the needle carrier 6 Check the alignment of the needle in the needle pusher of the needle carrier by viewing from several directions to see that it is aligned in the center of the needle pusher The needle must be centered in the needle pusher as all alignment by the autosampler is calculated from the needle pusher position 7 Remove the silicon safety tube from the needle 8 Replace the plate tray in the tray base 9 Re install the side door and close the front door 10 In the user interface close the Change Needle Carrier function and exit the maintenance mode The instrument will reset In the Agilent Lab Monitor amp Diagnostic Software the Change Needle Carrier function can be found in the Tools icon 1200 Series Autosamplers User Manual 111 7 Maintenance Exchange the Needle Seat Assembly G1367 87101 on the G1367B Samplers When Tools required Parts required 112 When the seat is visibly damaged When the seat capillary is blocked 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 4 mm open end wrench 8710 1534 supplied in accessory kit Flat screwdriver Partnumber Description G1367 87101 Needle Seat assy 0 17 mm ID 2 3 pl for G1367B In the user interface start the maintenance mode and select the Change Needle Seat function In the Agilent Lab Monitor amp Diagnostic Softwarethe
75. losionsfare ved fejlagtig h ndtering Udskiftning ma kun ske med batteri af samme fabrikat og type gt Lever det brugte batteri tilbage til leverand ren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten gt Brukt batteri returneres appararleverandoren is Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1200 Series Autosamplers User Manual 145 9 Appendix The Waste Electrical and Electronic Equipment Directive Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all electric and electronic appliances starting with 13 August 2005 This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control Instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 146 1200 Series Autosamplers User Manual Appendix 9 Radio Interference Never use cables other than the ones
76. m if m Tawo moo casa m o m Remote 7 Tf RS232C DC Out m C C CAN bus E C CD OC C cS OC Cc ce papa CAN cable to next module Figure 12 Cable Connections NOTE The sampler is turned ON when the line power switch is pressed and the green indicator lamp is illuminated The sampler is turned OFF when the line power switch is protruding and the green light is OFF 44 1200 Series Autosamplers User Manual Installing the Autosampler 3 Installing a Thermostatted Autosampler Parts required Description 1 Sampler and thermostat Power cord Preparations Locate bench space Provide power connections Unpack the sampler and the thermostat WARNING Instrument is partially energized when switched off The power supply still uses some power even if the power switch at the front of the panel is turned off gt To disconnect the sampler from the line unplug the power cord WARNING Damaged electronics Disconnecting or reconnecting the sampler to thermostat cable when the power cords are connected to either of the two modules will damage the electronics of the modules gt Make sure the power cords are unplugged before disconnecting or reconnecting the sampler to thermostat cable WARNING Damage through condensation If the condensation tube is located in liquid the condensed water cannot flow out of the tube and the outlet is blocked Any further condensation will then remain in the
77. maintenance procedures require the needle arm metering device and needle carrier to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position For details refer to Maintenance Functions on page 89 102 1200 Series Autosamplers User Manual Maintenance 7 Early Maintenance Feedback EMF Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user setable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The autosampler provides four EMF counters Each counter increments with autosampler use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The autosampler provides the following EMF counters Injection Valve Counter This counter counts the number of valve switching EF4512 since the last reset of the counter Needle Assembl
78. nce 11 Injection Sequence 12 Sampling Unit 15 Analytical Head 16 Injection Valve 16 Needle Flush Station 17 Needle Lock 17 Needle Sample Transport Assembly 18 Advanced Operating Modes 20 Early Maintenance Feedback EMF 21 Electrical Connections 22 8 ee Agilent Technologies 1 introduction Introduction to the 1200 Series Samplers Three models of Agilent 1200 Series samplers are available that handle vials well plates and Eppendorf tubes e G1367B Agilent 1200 Series high performance autosampler e G1367D Agilent 1200 Series high performance autosampler SL e G1377A Agilent 1200 Series micro well plate autosampler The G1367B Agilent 1200 Series high performance autosampler adds maximum flexibility and fast injection cycles whenever high sample throughput and speed of analysis are required Features Increased sample injection speed for high sample throughput overlapped injections for increased productivity minimal delay volumes for rapid gradients and fast equilibration when bypassing the autosampler after sample injection flexible and convenient sample handling with different types of sample containers Using 384 well plates allows to process up to 768 samples unattended The G1367D Agilent 1200 Series High Performance Autosampler SL is specifically designed for the Agilent 1200 Series Rapid Resolution LC system for increased analysis speed with uncompromised sensitivity resolution precision and lowest carry over
79. needle assembly with the loop capillary from the needle carrier Attach the 5 16 inch wrench to hold position at the needle assembly Use the 4 mm wrench to loosen the fitting of the loop capillary 1200 Series Autosamplers User Manual Maintenance 7 Maintenance Procedures NOTE Do not bend the sheet metal of the needle 10 Pull the loop capillary out from the needle assembly 1200 Series Autosamplers User Manual 107 7 Maintenance Installing the Needle Assembly When When the needle is visibly damaged When the needle is blocked Tools required Two 1 4 inch 5 16 inch wrenches 8710 0510 supplied in accessory kit 4mm open end wrench 8710 1534 supplied in accessory kit Parts required Partnumber Description 1 G1367 87202 Needle assembly for G1367B D 1 G1377 87201 Needle assembly for G1377A WARNING Risk of injury by uncovered needle An uncovered needle is a risk of harm to the operator gt Be careful when you remove the needle assembly gt Use the silicon safety tube supplied with every new needle 1 Push the silicon safety tube supplied in the WPS leak kit G1367 60006 and with every new needle over the needle 2 Pull the loop capillary in the new needle assembly G1367 87202 or G1377 87201 3 Attach the 5 16 inch wrench to hold position at the needle assembly Use the 4 mm wrench to tighten the fitting of the loop capillary Do not hold the needle during this step to avoid to bending it
80. ng recommendations on the use of solvents e Brown glass ware can avoid growth of algae e Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid and nitric acid especially at higher temperatures if your chromatography method allows replace by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCly O gt 2COCI 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solvents containing strong complexing agents e g EDTA Mixtures of carbon tetrachloride with 2 propanol or THF 1200 Series Autosamplers User Manual 149 9 Appendix Agilent Technologies on the Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will also provide the latest firmware of the Agilent 1200 series modules for download
81. nly when the front cover is closed Initialization takes about 1 5 minutes When initialization is complete the needle is in the needle seat the needle lock is down and the status indicator is off Figure 21 Instrument LED indicator 1200 Series Autosamplers User Manual 1200 Series Autosamplers User Manual e 5 Optimizing Performance Optimizing Performance 66 Optimization for Lowest Carry Over 67 Using the Automated Needle Wash 70 Using the Flush Port 70 Cleaning the needle seat 71 Fast Injection Cycle and Low Delay Volume 74 Precise Injection Volume 76 Draw and Eject Speed 76 Choice of Rotor Seal 79 Choice of Seat Capillary 80 ee Agilent Technologies 65 5 Optimizing Performance Optimizing Performance 66 Autosamplers are more and more used in HPLC to improve the productivity in the laboratories and the consistency and accuracy of analytical results The informations below will help you on how to optimize some parameters to achieve best results for e lowest carry over for reliable quantitative data e Fast injection cycles for high throughput e Low delay volume for fast gradient e Precise injection volume Specific information for optimizing the performance of the Agilent 1200 series high performance autosampler SL can be found in the Agilent 1200 Series Rapid Resolution LC System User Manual 1200 Series Autosamplers User Manual Optimizing Performance 5 Optimization for Lowest Carry Over Optimiz
82. notice until the indicated condi tions are fully understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1200 Series Autosamplers User Manual Contents Contents Introduction 7 Introduction to the 1200 Series Samplers 8 Sampling Sequence 11 Sampling Unit 15 Needle Sample Transport Assembly 18 Advanced Operating Modes 20 Early Maintenance Feedback EMF 21 Electrical Connections 22 Site Requirements and Specifications 23 Site Requirements 24 Physical Specifications 27 Performance Specifications 28 Installing the Autosampler 33 Unpacking the Sampler 34 Optimizing the Stack Configuration 37 Installing the Autosampler 42 Installing a Thermostatted Autosampler 45 Flow Connections to the Sampler 49 Installing the Sample Tray 51 Transporting the Sampler 53 Using the Autosampler 55 Sample Trays 56 List of Recommended Plates and Closing Mat 57 List of Recommended Vials and Caps 59 Configure Well Plate Types 61 Turn ON and Initialization Steps 64 Optimizing Performance 65 Optimizing Performance 66 Optimization for Lowest Carry Over 67 1200 Series Autosamplers User Manual Contents Fast Injection Cycle and Low Delay Volume 74 Precise Inje
83. ntenance Procedures Maintenance 7 The procedures described in this section can be done with the autosampler in place in the stack These procedures can be done on a more frequent basis Table 23 Simple Repair Procedures Procedure Typical Frequency Notes Exchanging the needle assembly Exchanging the needle carrier assembly Exchanging the needle seat assembly Exchanging the stator face Exchanging the rotor seal Exchanging the metering seal Exchanging the loop capillary Exchanging the peristaltic pump When the limit in the needle into seat counter in the EMF is exceeded When needle shows indication of damage or blockage When the needle carrier is defective When the limit in the needle into seat counter in the EMF is exceeded When needle seat shows indication of damage or blockage When the valve performance shows indication of leakage or wear When the limit in the injector valve switches counter in the EMF is exceeded When the valve performance shows indication of leakage or wear When autosampler reproducibility indicates seal wear When loop capillary blocked or broken When tubing broken See Removing the Needle Assembly on page 106 See Removing the Needle Carrier Assembly on page 110 See Exchange the Needle Seat Assembly G1367 87101 on the G1367B Samplers on page 112 See Stator Face on page 116 See Rotor Seal on page 117 See Metering
84. oned over the programmed sample position the programmed sample volume is drawn by the metering device into the sampling needle The sampling arm then moves to the injection position where the sample is flushed onto the column The autosamplers employ a vial plate pusher mechanism to hold down the vial or the plate while the needle is drawn back from the sample vessel a must in the case a septum is used This vial plate pusher employs a sensor to detect the presence of a plate and to ensure accurate movement regardless of plate used All axes of the transport mechanism x z theta robot are driven by stepper motors Optical encoders ensure the correct operation of the movement The standard metering device for the G1367B provides injection volumes from 0 1 100 ul A multi draw kit extends the range up to 1500 ul A 0 1 40ul injection volume metering device is installed in the G1367D with the option to install an extended volume metering device and loop capillary with up to 100ul injection volume The micro metering device for the G1377A provides injection volumes from 0 01 8 ul with the standard loop capillary installed and from 0 01 40 ul with the extended loop capillary The entire flowpath including the metering device is always flushed by the mobile phase after injection for minimum internal carry over An additional needle flush station with a peristaltic pump is installed to wash the outside of the needle This reduces the already
85. ow For standard applications no additional flushing procedure is required 1200 Series Autosamplers User Manual Introduction 1 Sampling Unit Sampling Unit The sampling unit consists of subsystems as well The main carrier part is a die casting part which carries the following functional elements Needle drive Needle lock Analytical head Flush port Needle seat Peristaltic pump Injection valve Figure 5 Sampling unit 1200 Series Autosamplers User Manual 15 1 16 Introduction Analytical Head The analytical head is driven by the stepper motor connected to the drive shaft by a toothed belt The drive nut on the spindle converts the circular movement of the spindle to linear motion The drive nut pushes the sapphire plunger against the tension of the spring into the analytical head The base of the plunger sits on the large bearing of the drive nut which ensures the plunger is always centered A ceramic ring guides the movement of the plunger in the analytical head The home position of the plunger is sensed by an infra red sensor on the sampling unit flex board while the sample volume is determined by counting the number of steps from the home position 7 nl motor step The backward movement of the plunger driven by the spring draws sample from the vial To reduce potential user mistakes different versions of analytical heads are recognized by RF tags sitting on the exchangeable assembly Table 1
86. problem which affects correct operation of the instrument Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis 1200 Series Autosamplers User Manual Troubleshooting and Diagnostics 6 Maintenance Functions Some maintenance procedures require the needle arm metering device and needle carrier to be moved to specific positions to enable easy access to components The maintenance functions move these assemblies into the appropriate maintenance position In the ChemStation the sampler maintenance positions can be selected from the Maintenance menu in the Diagnosis display In the Control Module the functions can be selected in the Test screens of the autosampler In the Agilent Lab Monitor amp Diagnostic Software the maintenance positions can be selected from the Tools icon Maintenance Functions The maintenance functions moves the arm assembly in a specific position in order to enables easy access for maintenance operations Home Position The home position function moves the arm to the right side for better access and exchange of the trays Park Position The park position function moves the arm to the left side of the tray In this position it is possible to secure the sample transport mechanism with the protection foam The sample transport is than ready for transporting Change Piston The change piston function draws
87. r Especially if the sample and sample solvent doesn t fit to the mobile phase an arbitrarily large amount of sample may miss the column but stay in the main pass The Injector Purge Kit was developed for this purpose During run the syringe is used as purge pump and afterwards exchanges the purge solvent with gradient start conditions Using this kit decreases the poor bypass carry over significantly Eventually main pass performance may be obtained But the kit does not resolve the problem of discriminating sample compounds Contraindication for usage e If run times are below 2 3min the purge kit won t help as the purge step with reasonable purge volume lasts at least 2min e In highly carry over sensitive applications purge kit is not recommended as best performance is got when the Autosampler stays in mainpass the full solvent gradient 1200 Series Autosamplers User Manual 69 5 70 Optimizing Performance Control software support for the Purge kit is provided and fully operated and controlled through the ChemStation or and G4208A Instant Pilot via the Autosampler Settings Requires Agilent ChemStation B 01 03 or higher and Firmware A 06 01 and higher on ALL Agilent 1200 Series modules that are part of the system and Firmware B 04 01 for the Control Module Using the Automated Needle Wash The automated needle wash can be programmed either as injection with needle wash or the needle wash can be included into the
88. ready run and error states of the instrument The status indicators provide a quick visual check of the operation of the autosampler see Status Indicators on page 87 Error Messages In the event of an electronic mechanical or hydraulic failure the instrument generates an error message in the user interface For details on error messages and error handling please refer to the Agilent Lab Monitor amp Diagnostic Software Maintenance Functions The maintenance functions position the needle assembly the needle carrier the sample transport assembly and the metering device for easy access when doing maintenance see Maintenance Functions on page 89 Sample Transport Self Alignment The sample transport self alignment with the sampling unit and the well plate tray is required to compensate for larger deviations in positioning the needle carrier The sample transport self alignment is required after disassembling the system or when you exchange the sample transport the sampling unit the tray or the MTP main board This function is in the diagnose screen of the Chemstation or the Control Module 1200 Series Autosamplers User Manual 85 6 Troubleshooting and Diagnostics Step Commands The step functions enable execution of each step of the sampling sequence individually The step functions are used primarily for troubleshooting and for verification of correct autosampler operation after repair see High Perfo
89. renches 1 4 5 16 inch 2 8710 0510 Wrench 4 0 mm open end 1 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 2391 Hex key 4 0 mm 15 cm long T handle 1 8710 2392 Hex key 9 64 inch 15 cm long T handle 1 8710 2394 Hex key 2 5 mm 15 cm long straight handle 1 8710 2412 Hex key 2 0 mm 1 8710 2438 ESD wrist strap 1 9300 1408 Torque adapter 1 G1315 45003 Air channel adapter 1 G1329 43200 Capillary sampler column 500 mm 0 05 mm ID 1 G1375 87304 40 ul Loop capillary 1 G1377 87300 WPS leak kit 1 G1367 60006 134 1200 Series Autosamplers User Manual Parts and Materials for Maintenance 8 Table 29 Micro Well Plate Autosampler Accessory Kit Contents G1377 68705 Description Quantity Part Number Seat capillary 150 mm 0 075 mm ID 1 G1367 87316 Tool for micro seat capillary 1 G1377 44900 1200 Series Autosamplers User Manual 135 Parts and Materials for Maintenance Multi Draw Kit G1313 68711 Only For G1367B 136 Table 30 Multi Draw Kit Item Description Part Number 1 2 3 Seat capillary 500 pl 0 5 mm id Seat capillary 1500 pl 0 9 mm id Union G1313 87307 G1313 87308 0100 0900 1200 Series Autosamplers User Manual Parts and Materials for Maintenance 8 G1373A Injector Purge Kit Parts Table 31 Injector Purge Kit Parts for G1367B Autosamplers Description Part Number Plastic cover cabinet kit with rail includes top and side 5064 1533 covers and the bolt carrier for mounting the
90. ription 5063 6589 Metering Seal pack of 2 for the G1367 60003 100 pl Analytical Head 5063 6586 Metering Plunger for the G1367 60003 100 ul Analytical Head 5022 2175 Metering Seal pack of 1 for the G1377 60013 40 pl Analytical Head 5064 8293 Metering Plunger for the G1377 60013 40 pl Analytical Head Install the new metering seal Press it firmly into position Reassemble the analytical head Press the plunger assembly into the seal Put the two fixing screws in place and reinstall the analytical head to the sampling unit 4 Tighten alternately the two fixing screws with the 4 mm hex key Connect the two capillaries to the analytical head Use a 1 4 inch wrench if you have a SST capillary or a 4 mm wrench if you have a fused silica capillary Reinstall the corrugated leak tubing Close the front door In the user interface close the Change Piston function and exit the maintenance mode In the Agilent Lab Monitor amp Diagnostic Software the Change Piston function can be found under the Tools icon 1200 Series Autosamplers User Manual Maintenance 7 Removing the Loop Capillary When Tools required Parts required i a m lm Capillary blocked Capillary broken Two 1 4 inch 5 16 inch wrenches 8710 0510 supplied in accessory kit Part number Description G1367 87300 Loop capillary injection volume up to 100 pl for the G1367B G1377 87310 Loop capillary injection volume up to 40 pl for the G1367D G1375
91. rmance ensuring minimum delay volume by installing the following configuration Figure 8 on page 38 and Figure 9 on page 39 show the configuration recommended for the sampler Figure 10 on page 40 and Figure 11 on page 41 show the configuration recommended for the thermostatted sampler 1200 Series Autosamplers User Manual 37 3 Installing the Autosampler Optimizing the Stack Configuration Solvent cabinet Vacuum degasser Instant Pilot Autosampler Column compartment Detector Figure 8 Recommended Stack Configuration Well Plate Autosampler Front View 38 1200 Series Autosamplers User Manual Installing the Autosampler Optimizing the Stack Configuration AC power Remote cable AC power CAN Bus cable AC power AC power AC power GPIB or LANto control software Figure 9 Recommended Stack Configuration Well Plate Autosampler Rear View 1200 Series Autosamplers User Manual 3 39 3 Installing the Autosampler Optimizing the Stack Configuration Detector Instant Pilot Column Compartment 1 Solvent Cabinet Autosampler Degasser ALS Thermostat Pump Figure 10 Recommended Stack Configuration Thermostatted Autosampler Front View 40 1200 Series Autosamplers User Manual Installing the Autosampler Optimizing the Stack Configuration GPIB or LAN Analog signal to recorder CAN bus cable Autosampler Thermostat cable Remotecable Analog s
92. rmance Autosampler Step Commands on page 91 For details on step commands please refer to the Agilent Lab Monitor amp Diagnostic Software 86 1200 Series Autosamplers User Manual Troubleshooting and Diagnostics 6 Status Indicators Two status indicators are located on the front of the autosampler The lower left indicates the power supply status the upper right indicates the autosampler status Status indicator green yellow red Pa Line power switch with green light Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions 1200 Series Autosamplers User Manual 87 88 Troubleshooting and Diagnostics When the status indicator is OFF and power switch light is on the instrument is in a prerun condition and is ready to begin an analysis A green status indicator indicates the instrument is performing an analysis run mode A yellow status indicator indicates a not ready condition The instrument is in a not ready state when it is waiting for a specific condition to be reached or completed for example front door not closed or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the instrument has detected an internal
93. ser Manual Optimizing Performance 5 e Connect the seat capillary with the pump capillary using a zero dead volume fitting Flushing e Increase pump flow The seat is flushed backwards solvent bubbles over the seat and leaves the seat via the drainage for the flush port Reconfigure system e Set pump flow to zero e Connect the pump outlet capillary to port 1 of injection valve e Connect the seat capillary to port 5 of the injection valve e Reset the injector 1200 Series Autosamplers User Manual 73 5 Optimizing Performance Fast Injection Cycle and Low Delay Volume Fast Injection Cycle and Low Delay Volume General 74 Short injection cycle times for high sample througput is one of the main issues in analytical laboratories Shortening cycle time starts with e shortening column length e high flow rate e steep gradient The detector balance may be set to OFF recommendations for Fast Injection Cycle Times As described in this section the first step to provide short cycle times is optimizing the chromatographic conditions Then the following Autosampler related issues should be considered e Use proper solvent for needle wash to decrease the wash time e Reduce injection volume e Increase eject speed e Increase draw speed if the viscosity of the sample and the solvent in Autosampler s flow path allows it e Do injection preparation in parallel with column equilibration section Overlapped Injection
94. sing the Autosampler Sample Trays 56 List of Recommended Plates and Closing Mat 57 List of Recommended Vials and Caps 59 Configure Well Plate Types 61 Turn ON and Initialization Steps 64 ee Agilent Technologies 55 4 Using the Autosampler Sample Trays Supported Trays for an Autosampler Table9 Trays for an Autosampler G2258 60011 Tray for 2 well plates or vial plates and 10 x 2 ml vials Pos P2 A1 Vial 10 Pos P2 B1 S Plate 2 Pos P2 P24 Pos P1 A1 Pos P1 B1 S Plate 1 Pos P1 H12 Vial 1 Figure 18 Numbering of vial and well plate position 56 1200 Series Autosamplers User Manual List of Recommended Plates and Closing Mat WARNING Explosive gas mixtures Using the Autosampler 4 There is a risk of building explosive gas mixtures in the instrument if flammable solvents are used gt Cover the plates gt Remove the plates from the sampler after turning it OFF WARNING Contamination with adhesives Closing mats with adhesive can give some contamination in the system The adhesive is soluble in most of the solvents used in HPLC gt In general do not use closing mats with adhesive The sampler has no prepunch needle therefore the adhesive will clog the needle after several injections Table 10 Recommended plates and closing mat Description Rows Columns Plate height Volume pl Part Number Package 384 Agilent 16 24 14 4 80 5042 1388 30 384Corning 16 24 14 4 80 No Agilent P
95. supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 1200 Series Autosamplers User Manual 147 9 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 148 1200 Series Autosamplers User Manual Appendix 9 Solvent Information Flow Cell To protect optimal functionality of your flow cell e Avoid the use of alkaline solutions pH gt 9 5 which can attack quartz and thus impair the optical properties of the flow cell Ifthe flow cell is transported while temperatures are below 5 degree C it must be assured that the cell is filled with alcohol e Aqueous solvents in the flow cell can built up algae Therefore do not leave aqueous solvents sitting in the flow cell Add a small of organic solvents e g acetonitrile or methanol 5 Use of Solvents Observe the followi
96. sy on the injection valve Tighten the screws alternately with the 9 64 inch wrench until the stator head is secure 7 Reconnect all the capillaries to the injection valve ports with the 1 4 inch wrench 8 Close the front cover 1200 Series Autosamplers User Manual Maintenance 7 Rotor Seal When Tools required Parts required CAUTION When poor injection volume reproducibility When leaking injection valve 1 4 inch 5 16 inch wrench 8710 0510 supplied in accessory kit 9 64 inch 15 cm long T handle hex key 8710 2394 supplied in accessory kit Partnumber Description 0100 1853 Vespel Rotor Seal for 0101 0921 injection valve G1367B 0100 1849 Tefzel Rotor Seal for 0101 0921 injection valve G1367B 0100 2231 PEEK Rotor Seal for 0101 0921 injection valve G1367B 0100 2088 Vespel Rotor Seal for 0101 1050 injection valve G1377A 0101 1416 PEEK Rotor Seal for 0101 1422 injection valve G1367D ae ae a l Damage of the stator face The stator face is held in place by the stator head When removing the stator head the stator face can be damaged gt When removing the stator head ensure the stator face does not fall out of the valve The injection valve 0101 1050 for the G1377A sampler has no stator face The injection valve 0101 1422 for the G1367D sampler has no stator face 1 Open the front door 2 Remove all capillaries from the injection valve ports with the 1 4 inch wrench 3 Unscrew and remove the thre
97. t the end of the sampling sequence 1200 Series Autosamplers User Manual 11 1 introduction The standard sampling sequence occurs in the following order The injection valve switches to the bypass position The plunger of the metering device moves to the initialization position The needle lock moves up The needle moves to the desired sample vial position The needle lowers into the vial The metering device draws the preset sample volume The needle lifts out of the vial The needle is then moved onto the seat to close the sample loop soo N OOo F OMN The needle lock moves down 10 The injection cycle is completed when the injection valve switches to the mainpass position If needle wash is required it will be done between step 7 and 8 Injection Sequence Before the start of the injection sequence and during an analysis the injection valve is in the mainpass position Figure 2 on page 12 In this position the mobile phase flows through the autosampler metering device sample loop and needle ensuring all parts in contact with sample are flushed during the run thus minimizing carry over Figure 2 Mainpass Position 12 1200 Series Autosamplers User Manual Introduction 1 When the sample sequence begins the valve unit switches to the bypass position Figure 3 on page 13 Solvent from the pump enters the valve unit at port 1 and flows directly to the column through port 6
98. tenance 8 High Performance Autosampler and SL Version Accessory Kit G1367 68705 Table 28 High Performance Autosampler and SL version Accessory Kit G1367 68705 Description Quantity Part Number Capillary sampler column 380 mm 0 17 mm ID 1 01090 87306 96 well plate 0 5 ml PP pack of 10 1 5042 1386 Tubing assembly 1 5063 6527 Filter kit 1 5064 8240 CAN cable 1 m 1 5181 1519 Vials screw cap 100 pk 1 5182 0716 Blue screw caps 100 pk 1 5182 0717 Hex key 9 64 inch for injection valve screws 1 8710 0060 Wrench 4 mm both ends 2 8710 1534 Rheotool socket wrench 1 4 inch 1 8710 2391 Hex key 4 0 mm 15 cm long T handle 1 8710 2392 Hex key 9 64 inch 15 cm long T handle 1 8710 2394 Hex key 2 0 mm 1 8710 2438 ESD wrist strap 1 9300 1408 Air channel adapter 1 G1329 43200 Capillary WPS to column 250 mm 0 17 mm ID 1 G1367 87304 WPS leak kit 1 G1367 60006 Tool for micro seat capillary 1 G1377 44900 1200 Series Autosamplers User Manual 133 8 Parts and Materials for Maintenance Micro Well Plate Autosampler Accessory Kit G1377 68705 Table 29 Micro Well Plate Autosampler Accessory Kit Contents G1377 68705 Description Quantity Part Number 96 well plate 0 5 ml PP pack of 10 1 5042 1386 Tubing assembly 1 5063 6527 Filter kit 1 5064 8240 CAN cable 1 m 1 5181 1519 Vials screw cap 100 pk 1 5182 0716 Blue screw caps 100 pk 1 5182 0717 Valve catalog 1 5988 2999 Hex key 9 64 inch for injection valve screws 1 8710 0060 W
99. th this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up A WARNING alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION A caution alerts you to situations that could cause loss of data and damage of equipment gt Do not proceed beyond a caution until you have fully understood and met the indicated conditions 144 1200 Series Autosamplers User Manual Appendix 9 Lithium Batteries Information Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IMDG is not allowed Danger of explosion if battery is incorrectly replaced gt Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries gt Replace only with the same or equivalent type recommended by the equipment manufacturer Lithiumbatteri Eksp
100. the piston away from the home position relieving the tension on the spring In this position the analytical head assembly can be removed and reinstalled easily after maintenance This position is also used to change the analytical head plunger and metering seal 1200 Series Autosamplers User Manual 89 6 Troubleshooting and Diagnostics Table 20 Maintenance positions Function Arm position in X Arm position in Arm Position in Z Note Theta Change Needle Left side Straight Up No current on Theta Change Carrier assembly Left side Straight Middle No current on the ST Change Loop capillary Middle Left Up Home position Right side Left rear Up Park arm Left side Right rear Up Sample Transport Self Alignment The sample transport alignment with the sampling unit and the well plate tray is required to compensate for larger deviations in positioning the needle carrier This function is in the diagnose screen of the Chemstation or the Control Module In the Agilent Lab Monitor amp Diagnostic Software this function is in the Calibration icon The sample transport self alignment is required after disassembling the system or when you exchange e The sample transport e The sampling unit e The MTP main board e The autosampler tray base 90 1200 Series Autosamplers User Manual Troubleshooting and Diagnostics 6 High Performance Autosampler Step Commands Each movement of the sampling sequence can be done under manual control
101. these requirements This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuseholders must be avoided 1200 Series Autosamplers User Manual Appendix 9 CAUTION Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy
102. ty 42 45 installing the thermostatted autosampler tray cover and front cover 53 instrument status indicator 87 internet 150 150 introduction to the autosampler 8 L low volume injections 76 M mainpass 12 maintenance functions 89 85 maintenance procedures 105 maintenance functions 102 step commands 91 maintenance procedures 21 metering device 76 metering device 15 missing parts 34 multi draw option 8 needle flush station 17 needle lock 17 151 Index needle drive 15 needle sample transport assembly 18 numbering of vials 56 0 operating modes 20 optimizing performance automated needle wash 76 delay volume 76 delay volume adjustment 76 early maintenance 76 inject valve seal 76 low volume capillary kit 76 P park transport assembly 53 parts and materials 34 vial trays and tray base 131 parts and materials accessory kitSL 133 accessory kit 136 maintenance kit 136 multi draw kit 136 136 thermostat 137 transport assembly 128 parts and meterials accessory kit micro well plate 134 Peek seal 79 performance specifications 28 physical specifications autosampler 27 power cords 25 25 power supply indicator 87 Power Consideration 24 power requirements 24 radio interference 147 152 repair procedures 101 repairs maintenance procedures 105 metering plunger 119 metering seal 119 needle assembly 106 rotorseal 117 using the ESD strap 99 rotor seal 79 S safety information on lithium batt
103. y Counter This counter counts the total number of needle into seat movements used for the needle life time EF4510 since the last reset of the counter Seat Assembly Counter This counter counts the total number of needle into seat movements used for the seat life time EF4511 since the last reset of the counter Peristaltic Pump This counter gives the accumulates active pump time in units seconds EF4513 1200 Series Autosamplers User Manual 103 7 Maintenance Using the EMF Counters The user setable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of autosampler components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EMF Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When instrument performance indicates maintenance is necessary make note of the values displayed by the injection valve and needle movements counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 104 1200 Series Autosamplers User Manual Mai

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Agilent 1200 Series High Performance Autosamplers & Micro Well