DEN-1B - User manual
Contents
1. a repair and maintenance technician Batteries Use alkaline preferred or rechargeable AA type batteries A ATTENTION DANGER risk of explosion and burns e The batteries must be inserted correctly with respect to polarity by following the diagram in the battery case If one battery is reversed two poles or two poles in contact with each other a chemical reaction is produced in minutes that releases explosive gasses and extremely corrosive liquid In case of doubt turn off the unit immediately and check the polarity Protect your eyes in case a leakage is detected Cover the battery case with a rag before opening it to avoid contact with any discharge Page 4 e In case of contact with liquid from the batteries rinse the affected area immediately with clear water and getimmediate medical attention e Donotmix brands of batteries e Do not mix new and used batteries e Remove the batteries from the unit for prolonged storage e Donotrecharge alkaline batteries e Donotshort circuit the batteries as this can cause burns e Donotattemptto open or dismantle batteries e Donotput used batteries ina fire e Keep batteries out of reach of children e Keep water out of the battery case e Follow the disposal instructions and properly dispose of the used battery DURING OPERATION Do not operate the unit in environments with aggressive or explosive chemical mixtures Do not operate the unit if it is faulty or ha2. does not cover in transit damage 3 2 Complete set Package contents Standard set DEN 1B Densitometer suspension turbidity detector 1 piece JAA LYP6 Dale A a iia 3 pieces external power supply unit ooooonnnccccnnninnocccccncnnnnccccncnannnncccnnnnannccnnninnnes 1 piece Operating Manual Certificate oooooccnnnnnicicininnooccccnnnnnoccccncnonnnnccnnnnno 1 copy Optional accessories A 16 adapter for tUe Skene ei Ee ETES on request CKG16 calibration kit for glass tubes 16 mm in diameter on request CKG18 calibration kit for glass tubes 18 mm in diameter on request 3 3 Set up e Battery setup Insert a flat sharp pin into the small socket according to the fig 1 1 on the underside and open the battery compartment Insert the batteries inside as shown on the installation scheme in the battery compartment e Place the unit on the horizontal even working surface e Alternatively connect the external power supply unit to the socket fig 2 2 on the rear side of the unit Fig 1 Bottom panel Page 7 3 4 Calibration The device is pre calibrated at the factory for operation with the glass tubes 18 or 16 mm in external diameter see the label on the bottom side of the unit at temperature range from 15 C to 25 C and saves calibration data when being switched off 3 4 1 3 4 2 3 4 3 3 4 4 3 4 5 3 4 6 3 4 7 Page 8 Note F
3. equipment claim shall be compiled certified and sent to the local distributor address Please visit www biosan lv Technical support section to obtain the claim form 7 4 The following information will be required in the event that warranty or post warranty service comes necessary Complete the table below and retain for your records Model Suspension turbidity detector Densitometer DEN 1B Serial number Date of sale Page 13 8 Declaration of Conformity Declaration of Conformity Equipment name DEN 1B Type of equipment Densitometer Directive EMC Directive 2004 108 EC Low Voltage Directive 2006 95 EC RoHS 2011 65 EC WEEE 2002 96 EC 8 2012 19 EU Manufacturer SIA BIOSAN Ratsupites 7 build 2 Riga LV 1067 Latvia Applied Standards EN 61326 1 Electrical eguipment for measurement control and laboratory use EMC reguirements General reguirements EN 61010 1 Safety reguirements for electrical eguipment for measurement control and laboratory use General reguirements We declare that this product conforms to the reguirements of the above Directive s Signature Svetlana Bankovska Aleksandr Shevchik Managing director Engineer of R amp D 12 06 2013 12 06 2043 Page 14 Biosan SIA Ratsupites 7 build 2 Riga LV 1067 Latvia Phone 371 6742 6137 Fax 371 6742 8101 http www biosan lv Version 1 04 October 2013
4. switching the unit on or off e It is recommended to keep the unit switched on for 15 min before starting the operation in order to stabilize itin the working mode e If flat bottomed tubes are used the solution level should be higher than 7 mm from a tube bottom if round bottomed tubes are used the level should be higher than 12 mm from a tube bottom e The display goes off if there is no tube in the socket during one minute Press the On key fig 3 3 to activate the unit Fig 3 General view Page 9 4 1 Ifan external power supply unitis used connect it to electric circuit 4 2 Switch ON the unit using the Power switch Fig 2 1 on the rear panel position 4 3 The following indication may be shown on the display fig 3 2 0 00 means that the unit is calibrated and ready for operation if no tube inserted CC flashing means that the unit is not calibrated Calibrate the unit LOW BATTERY means that change the batteries following the instructions of the Safety Precautions section concerning batteries 4 4 Shake the tube with the solution it is recommended to use a vortex for shaking e g V 1 plus personal vortex and insert it into the socket of Densitometer fig 3 1 The McFarland value forthe solution will be shown on the display fig 3 2 4 5 Glass and transparent plastic tubes 16 or 18 mm in external diameter can be used for work with densitometer An A 16 adapter must be inserted in the sock
5. MD Medical Biological Research amp Technologies Densitometer DEN 1B suspension turbidity detector Operating Manual for version Certificate V 1AW Contents Safety Precautions General Information Getting Started Operation Specifications Maintenance Warranty and Claims 0 NOV PO DMN Declaration of Conformity Page 3 1 Safety Precautions The following symbol means Caution Make sure you have fully read and understood the present Manual before using the equipment Please pay special attention to sections marked by this symbol GENERAL SAFETY Use only as specified in the Operating Manual provided The unit should be saved from shocks and falling After transportation or storage keep the unit under room temperature for 2 3hrs before connecting it to the electric circuit Use only cleaning and decontamination methods recommended by the manufacturer Do not make modifications in design of the unit ELECTRICAL SAFETY Connect only to a external power supply unit with voltage corresponding to that on the serial number label Use only the external power supply unit provided with this product Ensure that the power switch and external power supply are easily accessible during use Disconnect the unit from the external power supply unit before moving If liquid penetrates into the unit disconnect it from the external power supply unit and have it checked by
6. McFarland unit range if necessary Both commercial standards e g produced by BioMerieux Lachema etc and the cell suspensions prepared in the laboratory can be used for calibration Table 1 Interpretation of McFarland Standard results into corresponding numeric values of bacterial suspension concentration and their optical density at 550 nm McFarland Composition Interpretation Standard Concentration Bacterial Theoretical optical BaSO concentration density at 550 nm 0 5 2 40x10 mol l 150x10 cells ml 0 125 1 4 80x10 mol l 300x10 cells ml 0 25 2 9 60x10 mol l 600x10 cells ml 0 50 3 1 44x10 mol l 900x10 cells ml 0 75 4 1 92x10 mol l 1200x10 cells ml 1 00 5 2 40x10 mol l 1500x10 cells ml 1 25 6 2 88x10 molll 1800x10 cells ml 1 50 Bacterial concentration depends on microorganism size The numbers represent an average value valid for bacteria For yeasts which are larger in size these numbers should be divided by about 30 Values correspond to optical densities of bacterial suspensions The BaSO solutions optical density values differ because the particle size and form differ from those of bacteria and lightis diffracted differently Page 6 3 Getting started 3 1 Unpacking Remove packing materials carefully and retain them for future shipment or storage ofthe unit Examine the unit carefully for any damage incurred during transit The warranty
7. er u em entered 3 x batteries AA type 914 Weight ii i ss 0 7 kg Accurate within 10 Optional Description Catalogue number accessories A 16 Adapter for tubes 16 mm in external diameter BS 050102 AK CKG16 Calibration kit for glass tubes BS 050102 BK 16 mm in diameter CKG18 Calibration kit for glass tubes BS 050102 CK 18 mm in diameter Biosan is committed to a continuous programme of improvement and reserves the right to alter design and specifications of the equipment without additional notice Page 11 6 Maintenance 6 1 If the unit requires maintenance disconnect the unit from the electric circuit and contact Biosan or your local Biosan representative 6 2 All maintenance and repair operations must be performed only by qualified and specially trained personnel 6 3 Standard ethanol 75 or other cleaning agents recommended for cleaning of laboratory equipment can be used for cleaning and decontamination ofthe unit Page 12 7 Warranty Reclamation information 7 1 The Manufacturer guarantees the compliance of the unit with the requirements of Specifications provided the Customer follows the operation storage and transportation instructions 7 2 The warranted service life of the unit from the date of its delivery to the Customer is 24 months Contact your local distributor to check availability of extended warranty 7 3 If any manufacturing defects are discovered by the Customer an unsatisfactory
8. et when working with tubes which have external diameter 16 mm Note The unit must be calibrated each time a tube type e g with different outer diameter bottom shape or different material transparent plastic tubes is changed 4 5 After finishing the operation switch OFF the unit using the Power switch position O Ifthe external power supply unitis used disconnect it from electric circuit Page 10 5 Specifications The unit is designed for operation in cold rooms incubators and closed laboratory rooms at ambient temperature from 4 C to 40 C and maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C Ov LIGNESOUFGE nee LED 52 Wavelength Ren a i a a Ta i ss A 565 15nm 5 3 McFarland unitrange u en ina een 0 0 15 0 5 4 Display resolution us444244400nnnnnnnnnnnnnnnnnnnnnnnnannnnnnnnnnnnnnnnannnn 0 01McF 5 5 Accuracy of the Tull SCale acosada 3 5 6 Measurementtime LL Laikas ias sti vaika osios sk ii io ei 1sec Sample VOlUME Fica iii ela rege 2 ml minimum 5 8 Recommended external diameter of tube ooooocccinocccnonacccnonncconanccnnnnnnnannnno 18 mm or using A 16 adapter 16 mm SN A O LCD 5 10 Dimensions ds sai 165x115x75 mm 5 11 Input current power CONSUMPTION uunesssnnnessnnnensnnnensnnnnen nn 12V 7mA 0 1W 5 12 External power supply unit input AC 100 240 V 50 60 Hz output DC 12 V 9 19 Batl
9. or work with other type tubes e g with different outer diameter bottom shape or different material transparent plastic tubes it is necessary to perform calibration in the specified tubes Perform calibration in the following sequence from the lower calibration value to the higher values 0 0 0 5 1 0 2 0 3 0 4 0 5 0 6 0 Use at least 2 points for calibration Attention Make sure thatthe tube socketis empty If the external power supply unit is used connectit to electric circuit Switch ON the unit using the Power switch Fig 2 1 on the rear panel position I Press the Select button fig 2 3 on the backside of the unit use a thin pin with maximum diameter of 2 mm to press the Select and Install buttons A flashing indication will be shown on the display showing that the unit is ready to save calibrations value ofthe first calibration point the empty socket Press the Install button fig 2 4 the empty socket calibration value will be saved in the unit memory and the next calibration value is displayed flashing figure 0 00 Note Shake the tube with the standard solution if necessary it is recom mended to use vortex e g Personal Vortex V 1 plus for shaking Insert the tube with the standard solution corresponding to the calibration point value into the socket fig 3 1 of Densitometer Press the Install button This calibration curve value will be saved in the memory and the next calibration value
10. s been installed incorrectly Do not use outside laboratory rooms The Select and Install buttons are used only for calibration of the unit according to p 3 4 Do not press the buttons in other cases as this can cause calibration reset and recalibration will be needed BIOLOGICAL SAFETY Itis the user s responsibility to carry out appropriate decontamination if hazardous material is spilt on or penetrates into the equipment Page 5 2 General Information DEN 1B Densitometer is designed for solution turbidity measurement in the range of 0 0 6 0 McFarland units 0 cells ml 1800x10 cells ml DEN 1B is capable of measuring solution turbidity in a wider range 6 0 15 0 McFarland units however it is necessary to remember that in this case the standard deviation values increase DEN 1B Densitometer is used for 1 determining concentration of cells bacterial yeast cells in the fermentation process 2 detection of susceptibility of microorganisms against antibiotics 3 identification of microorganisms with various test systems 4 measuring optical density at fixed wavelength and 5 quantitative evaluation of concentration of dyed solutions that absorb green light The operation principle is based on optical density measurement with digital result representation in McFarland units The unit is calibrated at the factory and saves calibration data when being switched off However in can be calibrated by 2 8 points in 0 0 6 0
11. will be displayed Note It is recommended to calibrate O value and as many points as possible to obtain precise results The minimum requirement is to calibrate 2 points closest to the working range limits e g O and 6 0 for operation in O 6 0 McF unitrange Repeat steps 3 4 5 3 4 6 until the calibration is complete 1 7 times i e as 12 34 Fig 2 Rear panel many times as many points the chosen calibration curve has 3 4 8 If a standard is not available press the Select button for the next calibration value to be displayed 3 4 9 After installing the last standard value 6 0 or skipping it by pressing the Select button the will exit the calibration mode automatically The unit is ready for operation Note If pressing the Install button during the calibration process does not cause switching to the next standard value it means that the currently inserted in the densitometer socket standard has lower turbidity value than the previous standard The reason is that the inserted standard solution turbidity does not correspond to the required value the standard is to be shaken or replaced 3 5 10 After finishing the calibration switch OFF the unit using the Power switch position O If an external power supply is used disconnect the external power supply unit from electric circuit 4 Operation Recommendations during operation e Remove the tube with the solution being measured before
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