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Cell Biology - Harvard Apparatus

1. _ Specialized Tools For Bioresearch BS4 64 0952 Yes 1 ERW F10P BS4 64 0953 12 ERW F12P GUES por BS4 64 0954 15 ERW F15P E BS4 64 0955 2 0 ERW F20P Vented models are standard with the Warner OC 725 Oocte Clamp M34 11 000 Specialty Products to Enhance Your Bioresearch Electrode Holders E Series Holders with Handle Holders include a 6 3 mm diameter X 6 3 cm long handle for mount ing in a micropositioner Handles are screwed together except for vented models which are joined by a pin and jack for easy removal from the handle E SERIES with Handle Catalog No Wire Pellet Connector Model BS4 64 1023 Pellet 2mm Jack E45P F10NH BS4 64 1024 E45P F12NH BS4 64 1025 E45P F15NH BS4 64 1026 E45P F20NH BS4 64 1039 Yes E45P F10PH BS4 64 1040 E45P F12PH BS4 64 1041 E45P F15PH BS4 64 1042 E45P F20PH BS4 64 1019 Wire 2 mm Jack E45W F10NH BS4 64 1020 E45W F12NH BS4 64 1021 E45W F15NH BS4 64 1022 E45W F20NH BS4 64 1035 E45W F10PH BS4 64 1036 E45W F12PH BS4 64 1037 E45W F15PH BS4 64 1038 E45W F20PH A BS4 64 1051 E45W F10VH oO BS4 64 1052 E45W F12VH BS4 64 1053 E45W F15VH wN BS4 64 1054 E45W F20VH Vented models are standard with the Warner OC 725 Oocte Clamp Oc lt U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardappara
2. E OS Gi VE a PL Di oN So Perfusion Inlet Port 3 8 S 17 0 mm lt _ L74 4 gt d pD D Wick Slot r ra Ye gt A gt G abe q Volume 143 ul D 4 h dT Volume is for 1 mm solution D ha Model RC 21BDW Chamber for 25 mm Round Coverslip 143 pl The RC 21BDW is an open chamber using the popular 25 mm round coverslip Constant solution level and quiet opera tion are assured with the use of a wick between the bath and suction well The RC 21BRW is used with either the P 2 or 7 A 2 CED Chamber for 25 mm Round Coverslip RC 21BRW 240 pl The RC 21BRW is a new addition to the Series 20 family It is an open chamber using the popular 25 mm round coverslip Constant solution level and quiet operation are assured with the use of a wick between the bath and suction well RC 21BRW is used with either the PH 2 Platform Bath volume is P 2 or PH 2 platform see page M112 Bath volume is 240 pl for 143 pl for 1 mm solution height Standard accessories supplied are 1 mm solution height 25 mm 1 coverslips and PE 160 Tubing 10 ft Catalog No Model Product Catalog No Model Product BS4 64 0227 RC 21BRW Open Chamber for 25 mm Round BS4 64 0362 RC 21BDW Open Chamber for 25 mm Round Coverslip Coverslips BS4 64 0278 P 2 Platform Accessories Replacement Parts BS4 64 0285 PH 2 Heater Platform BS4 64 0705 CS25
3. M19 Respirometry Specialized Tools For Bioresearch M20 C Microcathode Oxygen Electrode Model 1302 m This is a Clark type e May be used with or without stirring polarographic elec the medium trode with a 22 micron diameter platinum cathode and __ silver silver chloride anode con nected by a buffered potassium chloride electrolyte solution In the normal configuration the cathode is cov ered with a relatively low permeability polypropylene membrane in order for the electrode to be used in unstirred solutions or where minimal stirring is required This membrane gives a relatively slow response time For fast response as required for rapidly respiring enzyme preparations a thin FEP membrane used with a special electrode jacket BS4 69 3011 is used Rapid stirring of the medi um is then necessary The electrodes are not temperature compen sated and require controlled temperature environments within 0 1 C They should always be used in the electrode holder of one of the Strathkelvin accessories so that only the tip of the electrode is exposed to the medium e High stability of output e Very low rate of oxygen consumption e Compatibility with all Strathkelvin accessories Specifications Response Time at 37 C Polypropylene Membranes FEP Membranes Oxygen Consumption Polypropylene Membranes c 18 sec for 90 change c 6 sec for 90 change c 0 5 3 0 x 10 mg O min Temperat
4. Discussion Each heating strategy discussed above has strengths and weakness es making it an incomplete solution for issues encountered with heating However most experimental difficulties can be solved by using these strategies in combination For example platform heating alone is not sufficient to stably heat a perfusing solution but is necessary if solution flow is to be inter rupted for any reason In addition the most effective method of heating a perfusion solution is via an in line heater Therefore an optimal combination for experiments where tight thermal control is desired under variable very slow or interrupted flow conditions would be a combination of in line and platform heating If solutions are stored cold and used before reaching operating temperature or if the temperature is changed during an experi ment then outgassing at the sample can be a significant problem This is addressed by including reservoir preheating in the experi mental setup However reservoir heating is unable to supply heat ed solutions to the chamber and is best used in combination with an in line solution heater Of course all three approaches can be used in combination to pro vide efficient thermal regulation under virtually any condition Warner Instrument Corporation provides high quality single and dual channel temperature controllers as well as solution reservoir chamber platform and in line solution heaters We invite you to call
5. The PLI 100 is very robust in constant use in our lab Most importantly the PLI 100 valves prevent cytosol and yolk backflow into my pipette after oocyte injection It s very easy to control The PLI 100 is heavily used on a daily basis and works great I like the fact that I can use the PLI 100 with TTL pulses It works great in conjunction with my imaging system The balance pressure is a good option My injection pipettes rarely clog Easy to Use Inject with the touch of a button or a tap of the foot switch it s as easy as that Reproducible Pressure to the pipette is controlled precisely through a multi turn regulator and is reported digitally for easy repeat Injection time is digitally controlled in 10 msec steps between 0 01 to 0 99 seconds and in 1 second steps between 1 to 99 seconds Pipette tip diame ter and hence delivered volume are easy determinations once the timing and pressure are known Versatile Deliver volumes from femtoliters to microliters with the same instru ment resulting in a wide range of applications Pressure Capabilities The PLI 100 features two negative and three positive pressure pneumat ic capabilities The negative or vacuum functions allow the user to 1 Fill micropipettes from their tips reducing wastage of valuable injectables 2 Provide a means to secure and manipulate a cell using a holding pipette The positi
6. All of the above factors enhance the precise delivery of sample e Membrane key pad e Improved micropipette holder e Two speed injection fill rates e Auto Home capability Volumes for the Nanoject II range from 2 3 nl to 69 nl Although previous models of the Nanoject series are excellent for Xenopus injections the smaller tips and smaller injection volumes of the Nanoject II allow users to perform injections into other specimens applications that previously could not be performed Positive displacement technology and the use of precision micropipettes eliminate the need for tedious calibration when the viscosity of the sam ple changes Contaminants in the sample do not hinder or change the injection volume as with some other injectors An optional universal mounting adapter provides easy mounting of the Nanoject II to a variety of micromanipulators and stereotaxic frames U S Toll Free 800 272 2775 e Fax 508 429 5732 Online www harvardapparatus com Universal Adapter AZoj01g 2D For the PLI 90 and PLI 100 Pico Injectors see pages M71 to M73 For HBIO Warner s Oocyte Clamping Products see pages H20 to H26 M77 Microinjection and Microperfusion M78 Micropipettes e deal for cell research e Compatible with all micromanipulator systems e Available in five bend angles e Gamma irradiated sterilization e Disposable These Micropipettes are ideal for s
7. C of the heating element and consequenily the perfusion solution as long as the feedback loop is sufficiently optimized Warner Instrument temperature controllers are provided with user selectable feedback response time constants allowing the instrument to accommodate different operating conditions 11 000 Specialty Products to Enhance Your Bioresearch Methodology In Line Solution Heating The simplest and most direct approach for the application of heat to a sample is to preheat the perfusion solution immediately prior to its delivery to the chamber The warmed perfusate washes over the sample and maintains a uniform temperature at the sample even with variable flow rates This method works well as long as solu tion continues to flow through the heater into the chamber A number of considerations are important when using this approach First it is important to minimize the distance between the heater and chamber If too much heat is lost en route the user can attempt to raise the heater controller set point above the boil ing point of the solution In addition care must be taken to main lain flow rates such that the solution temperature reaches equilib rium prior to leaving the heater Chamber Platform Heating Another strategy is to heat the chamber platform This is usually achieved by using a resistive element and this approach is provided for in Warner chambers A slightly more sophisticated tactic would be to supply heat to
8. Catalog No Product BS4 69 0075 Protelyte Reference Electrolyte Solution 100 ml BS4 69 0076 Electrode Storage Solution 500 ml BS4 69 0077 Electrode Connecting Cable BNC to AS7 1 m 3 3 ft BS4 69 0078 Electrode Connecting Cable DIN to AS7 1 m 3 3 ft BS4 69 0079 Electrode Connecting Cable Metrohm Lemo to AS7 Type F 1 m 3 3 ft BS4 69 0080 Electrode Connecting Cable Metrohm Lemo to AS7 Type H 1 m 3 3 ft BS4 69 0081 Electrode Connecting Cable Radiometer to AS7 1 m 3 3 ft BS4 69 0082 Electrode Connecting Cable Standard US to AS7 1 m 3 3 ft For DURACAL Buffer Solutions see page M27 For the AMANI series of LEAK FREE pH Electrodes see pages M24 For Microminiature pH Electrodes O Electrodes and CO Electrodes see pages M23 and M25 11 000 Specialty Products to Enhance Your Bioresearch pH and Reference Electrodes DURACAL Buffers Sas 0k PAs Accurate stable buffers are critical for the calibration of pH and se T redox electrodes to achieve accurate measurements particularly with modern pH meters incorporating GLP functions for recording elec trode performance DURACAL pH and Redox Buffer solutions are the buffers you can trust They offer you many advantages over tradi tional buffer solutions guaranteed five year stability excellent stabil ity of even pH 9 21 in air see Figure 1 automatic buffer recognition so you use the correct buffer to calibrate All of this in a unique tim
9. Dimensions L x D 25 4 x 0 9 mm 1 x 0 035 in Product Combination pH Electrode in 20 ga Needle Catalog No BS4 69 0493 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 2D M23 pH and Reference Electrodes Specialized Tools For Bioresearch M24 AMANI LEAK FREE Microcombination pH Electrodes LEAK FREE AMANI 650 1000 1000L Series pH Sensor AMANI IV 18 5 ga Duck Head Stainless Steel Needle Duck Head The AMANI LEAK FREE series of non glass micro pH electrodes are the latest development in our combination built in reference pH electrode line The new technologies used in these electrodes provide superior sensitivity and stability The LEAK FREE non porous reference junction eliminates the problems of clogged junction and adsorption of proteins and surfac tants They also have high resistance to chemical including hydrofluoric acid solutions With tip diameters starting as low as 650 pm they are one of the smallest combinations pH electrodes on the market today The new DUCK HEAD design of the AMANI IV has been devel oped to offer complete protection for the electrode s tip during insertion without compromis ing the electrodes performance The electrode is built into a custom 18 5 ga 1 2 mm nee dle and can be used to measure the pH of solid samples such meats fruits and vegetables without sample p
10. Electroincorporation Long duration electroporation consistent with the ECM 630 plat form has resulted in higher expression levels in COS 7 EDR3 DG44 and CV1 cell lines The ECM 630 will duplicate the five fold increase in transfection efficiency in comparison to calcium phos phate precipitation for COS 7 reported by Ishmael et al as well as the murine T Lymphoma results reported by Sundback et al Bacterial and Yeast Electroporation The ECM 630 is the most flexible bacterial and yeast electroporator available and will reproduce protocols by Beggs et al for Mycobacterium avium Sanders et al for Haemophilus influen za and Haemophilus somnus and Faber et al for Hansenula polymorpha 11 000 Specialty Products to Enhance Your Bioresearch Electroporation Products ECM 630 Exponential Decay Wave Electroporation System Plant Applications Intact plant tissue and protoplasts may be transformed using the ECM 630 including the species Agrostis palustris creeping bentgrass and Lycopersicon esculentum tomato as well as tobacco pollen trans formation Other Cell Types Exponential decay electroporation has been demonstrated to be superior to microinjection for medaka oocytes Yee et al transfect ed and expressed firefly Luciferase in Giardia lamblia In addi tion Quillan et al electrotransfected Xenopus laevis melanophores and fibroblasts Specialty Electrode Applications The BTX Choice Takac
11. Physiol 79 6 2050 2057 6 Olano M Song D Murphy S Wilson D E and Pastuszko A 1995 Relationships of dopamine cortical oxygen pressure and hydroxyl radicals in brain of newborn piglets during hypoxia and posthypoxic recovery J Neurochem 65 1205 1212 7 Cerniglia GJ Wilson D E Pawlowski M Vinogradov S A and Biaglow J 1997 Intravascular oxygen distribution in subcutaneous 9L tumors and radiation sensitivi ty J Appl Physiol 82 6 1939 1945 8 Rumsey WR Abbott B Lo L W Vinogradov S A and Wilson D E 1997 Imaging of oxygen in the surface and deep areas of the kidney Adv Exptl Med Biol 411 591 596 Specifications Detection Range Sensitivity Temporal Resolution Temperature Dependence Power Dimensions Weight Catalog No BS4 69 0502 BS4 69 0503 BS4 69 0504 BS4 69 0505 BS4 69 0506 BS4 69 0507 BS4 69 0508 BS4 69 0509 BS4 69 0510 BS4 69 0511 BS4 72 6180 BS4 72 6181 Air Saturation to lt 0 1 Torr 1 part in 10 Minimum 20 msec with measurements repeatable each 1 2 sec Approximately 3 per degree Celsius Wall Transformer 100 230 VAC 50 60 Hz 26 x 33 7 x 14 cm 10 25 x 13 25 x 5 5 in 5 kg 11 Ibs Product PMOD 2000 Phosphorescence Oxygen Measurement System Cool Color Lamp Amber 592 nm maximal intensity 115 VAC 60 Hz Cool Color Lamp Amber 592 nm maximal intensity 230 VAC 50 Hz Cool Color Lamp Red 638 nm maximal intensity 115 VAC
12. Respiratory inhibitors or other solutions can be injected directly into the cell via this slot using a fine bore needle included The volume of the cells may be set between 1 and 3 ml by rotating the knurled collar of the holders The rear of the bath contains a further 6 cells which are used to park the electrode hold ers and maintain them at a constant temperature while changing the con tents of the respiratory cells For easy removal and cleaning the cells are sealed into the water bath by O rings Diameter of Cells 16 mm 1 to 3 ml adjustable Cell Precision bore glass e Integral unit complete with magnetic stirrer e Good visibility of cell contents e Variable cell volume Volume of Cells Electrode Holder Bath Magnetic Stirrer Black acetal with peek tip Clear and black acrylic 6 position 12 VDC from external plugtop power supply 100 230 V 47 63 Hz Model RC 650 Product 6 Electrode Respirometer Catalog No BS4 69 3021 Respirometry Respiration Cells RC 300 and RC 350 EJ These respiration cells are for e small sample volume small volume measurements of oxygen uptake rate by microbial suspensions cell suspensions mitochondria or enzyme prepa rations The electrode holder supplied with the cell slides into one of two glass tubes fused into the glass water jacket The second tube is used to park the electrode holder when changing solutions A fine slot 1 x 1 mm in the side of the hold
13. equilibrating pressure can be maintained to offset the effects of capillary action A conveniently situated release button on top of the screw control is provided to achieve equilibration Product screw Actuated Air Syringe Catalog No BS4 69 0105 Screw Actuated Micrometer Driven Hamilton Syringe This screw actuated syringe is a micrometer actuated syringe mechanism mounted on a small e deal for oil filled injection aspiration e Easy to use base This syringe is for e Hamilton syringe oil air assisted micro injections Incorporated and micro aspirations Its com e Accurate pact dimensions enable it to be ergonomically positioned next to the control lever of a micromanip ulator to facilitate single handed operation of both instruments e Sensitive control e Low dead air volume e High suction pressure e Easy to exchange or replace the syringe Suction injection is achieved by using the rotary actuator with a fine micrometer thread which has a 15 mm movement range A Hamilton 1 ml Gastight glass syringe with a Luer taper is incorporated A Luer taper hypodermic needle is mounted on the taper of the Hamilton syringe The syringe is connected by hard polyethylene tubing to a micropipette which in turn may be fitted to a micromanipulator or similar instrument The 1 ml Hamilton syringe can be easily exchanged for any other Hamilton 1700 syringe series including the 1000 500 200 100 and 50 pl sizes see
14. ference between the two The amplifier measures H x W x D 10 8 x 26 7 x 22 2 cm 4 25 x 10 5 x 8 75 in and weighs 1 6 kg 3 5 lbs It oper ates on 120 V AC current but can set for 220 V operation at the factory Product Dual Channel Oxygen Amplifier Catalog No BS4 56 5077 Electrode Chlorider Our chlorider simplifies the process of electrically depositing a new layer of Ag AgCl on the pure silver tip of our electrodes This layer can become contaminated and will wear off under normal operating conditions To rechloride an electrode simply fill the stainless steel cup with KCl solu tion and dip the electrode The chlorider also includes extra electrode O rings an O ring installation tool and various polishing papers Catalog No Product BS4 61 0138 Chlorider and Accessory Kit Oxygen System Replacement Parts BS4 61 0249 Miniature Magnetically Coupled Stirring Kit Compatible with BS4 56 4930 Batch Cell Chamber controlled by any Instech Stirring Controller Includes 12 mm Motor Assembly Mounting Clip and Teflon Stirring Bar BS4 56 5028 Accessory Kit for Electrode Maintenance Includes 15 O Rings O Ring Installation Tool 24 in Polishing Paper 24 in Scotch Brite and 72 in Membrane Material BS4 56 5036 Electrode Cable 107 cm 42 in for Instech Amplifiers BS4 61 0139 Electrode Cable 107 cm 42 in for YSI Amplifiers Replaces YS 5358 BS4 61 0140 Output Cable 107 cm 42 in for Instech Amplifiers
15. needle locating collar Alternatively the cell can be used as a micro flowcell by connecting the outflow from a respiring preparation The sample leaves the chamber through a glass Luer fitting to which a small bore flexible drainage tube can be connected The sample chamber is completely surrounded by water circulated through the glass water jacket from a constant temper ature bath This provides good temperature regulation while providing excellent visibility of the contents of the chamber The water jacket screws into an acetal base section and can be easily removed for clean ing The microcathode electrode fits into the central electrode holder and the membrane at its tip forms the floor of the sample chamber Sample Chamber 70 ul Overall Height 106 mm Jacket and Cell Glass Base Section Black acetal Catalog No Model Product BS4 69 3016 MC 100 MicroCell Model ci Flow Cell FC 100 e For continuous in line oxygen monitoring or for connection to flow through respirometer chamber e Easily disassembled for cleaning e Good visibility of flowline The acrylic chamber of the flowcell screws onto the elec trode holder Only the tip of the electrode projects into the water flow The inlet and outlet tubes are made from stainless steel The electrode holder is fitted with a col lar for attachment to a vertical or horizontal 1 4 to 3 8 inch diameter laboratory rod to enable the flowcell to be immersed below the surface of
16. ple matter Fluid is delivered to a perfusion inlet port and removed from the chamber by means of a height adjustable aspirator Volume 110 ul Volume 220 ul Chambers are available to fit the Willco Wells and the Corning or Falcon 35 mm dishes The chamber is compatible with Warner s DH 35 Culture Dish Heater and the DH 35i Culture Dish Incubator Volumes are for 1 mm solution height Catalog No Model Product BS4 64 0347 RC 37W Chamber Insert for Willco Corning 35 mm Dishes BS4 64 0348 RC 37F Chamber Insert for Falcon Corning 35 mm Dishes U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M89 Chambers for 35mm Cell Culture Dishes Models RC 37WC and RC 37FC Closed Bath Chambers Top Coverslip Perfusion Retainer Outlet Port Perfusion Inlet Port RC 37FC O RC 37WC e Designed for glass chamber inserts makes bottom 35 mm dishes perfusion of cell culture dishes a m e Permits imaging and simple matter The closed bath s perfusion in the cell promotes an even and A culture dish continuous solution exchange k e Incorporates Warner s and eliminates changes in focus i diamond fluidics due to alterations in bath height A j o flat OTSA INE Studies involving physiological N bicarbonate buffers can be E performed with no gas loss at T the chamber interface Fluid is delivered to a 18 gauge perfusion inlet port and removed from the chamber
17. 11 000 Specialty Products to Enhance Your Bioresearch BS4 69 0153 8 Channel Pressure Injection System e Multiple function system for micro injection and perfusion e Up to 8 injection perfusion outputs one hold cell output and one synchro nized drain out channel e Programmable timers counters and step sequences which can be stored for reuse e Manual control or automatic sequential step cycle operation e Front panel display for pressures and program ming information Microinjection amp Microperfusion Multi Channel Pressure Injection Systems aA ee The PM 4 and PM 8 programma ble 4 and 8 Channel Pressure Injector systems are designed for one to eight channel intracellular injection and extracellular perfu sion These microinjection and perfusion engines are especially designed for pharmacological drug testing molecular biological DNA RNA transferring intracytoplasmic sperm injection and cell electro physiological applications Combining an advanced micro controller with precision pneu matic components these systems can simultaneously control up to eight injection micropipettes or eight perfusion vials and one cell hold ing pipette and one drain pipette The four PM 4 or eight PM 8 injection perfusion output ports can be controlled separately or com H D oE N HAD y DoE Benoa jig CE bined together to perform actions such as injection capillary action bal ancing suction
18. 120 mm Hg It is only below a so called critical pO here at 1 mm Hg that the oxygen con sumption decreases with decreasing available oxygen Electrical stimu lation of the cells at a frequency of 9 Hz produces a 3 fold increase in oxygen consumption rhombus Here again the oxygen consumption remains constant over a wide pO range The critical pO is raised to 10 mm Hg Below this pO the oxygen consumption decreases with decreasing available oxygen Using the Oxystat system it could thus be shown that stimulation not only produces an increase in oxygen con sumption but also that the critical oxygen value below which the cells are in oxygen deficit is displaced towards higher partial O pressures References D Stumpe T and Schrader J Phosphorylation potential adenosine formation and criti cal PO2 in stimulated rat cardiomyocytes Am J Physiol 273 H756 H766 1997 Respirometry NO io S amp S O VO nmol min e mg Protein Figure 4 Oxygen consumption VO of isolated cardiomyocytes at different values of available oxygen Oxystat System For a complete Oxystat System please order one each of the following components BS4 73 0117 BS4 73 0118 or BS4 73 0119 BS4 73 0120 BS4 73 0122 BS4 73 0124 C BS4 50 1932 or BS4 50 1940 Catalog No Product og BS4 73 0117 Oxystat Chamber Thermostated Volume 10 ml o with Built In Stirrer Requires BS4 73 0118 or BS4 73 0119 Power Supply f
19. 16 gauge adjustable suction tube An agar bridge well has been added to accommodate a bath ground electrode All chambers mount in the QE 1 Quick Exchange Platform Chambers are supplied with one box of 1 5 thickness 25 mm cov erslips and spare O rings Additionally RC 47FSLP is supplied with 10 feet of PE 160 Polyethylene Tubing U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com BATH DIMENSIONS All chambers use 25 mm coverslips RC 46SNLP 4mm Volume RC 46SLP Volume RC 47FSLP Volume 118 pl Agar Bridge Well ASoj01g 2D Volumes are for 1 mm solution height Catalog No Model Product BS4 64 0372 RC 46SLP Quick Change Chamber Slotted Bath BS4 64 0373 RC 46SNLP Quick Change Chamber Narrow Slotted Bath BS4 64 0374 RC 47FSLP Quick Change Chamber w Field Stimulation Accessories Replacement Parts BS4 64 0375 QE 1 Quick Exchange Heated Base for RC 40 Series BS4 64 0705 CS25R Coverslips 25 mm Dia 1 Box 100 BS4 64 0715 CS25R15 Coverslips 25 mm Dia 1 5 Box 100 BS4 64 1424 SIU 102 Stimulus Isolation Unit BS4 64 1425 CC 102 Cable male banana to 1 mm jacks for use with Warner field stimulation chambers M85 Micro Incubation Model QE 1 Quick Exchange Heated Platform with Perfusion Quick Exchange Platform The Warner QE 1 Quick Exchange Platform is a versatile base for the RC 40 Series chambers as well as many popular 35 mm glass bot tom
20. 1A from external plugtop power supply 100 230 VAC 47 63 Hz Dimensions 185 x 135 x 105 mm 7 25 x 5 25 x 4 125 in Catalog No Model Product BS4 69 3001 782 1 2 Channel Oxygen Meter 11 000 Specialty Products to Enhance Your Bioresearch C Respirometry Software The software used in the 782 meter and the 928 system is essential ly similar The main difference is that the 928 displays up to 6 traces while the 782 has a maximum of two The program has been written so that it is extremely user friendly and quick to learn It can be used for closed cell respirometry with the MT200 RC300 RC350 or RC650 or flow respirometry using the FC100 or MC100 or for monitoring dissolved oxygen using the FC 100 or MC100 When used for respirometry experiments it fol lows the familiar logical sequences of operations from electrode calibration to recording and analysis The program follows a sequence of 3 main stages Setting up Recording and Analysis Setup The setup screen is used for entering all of the details which the com puter needs in order to record in the correct units and to express the respiration rate normalized to biomass units if required in the desired units All experimental details entered here are transferred through to the final calculated results report page During setup the cell volumes for closed cell and flow rate electrode positioning for flow respirometry temperature of the experiment scrolling speed o
21. 867 870 1998 2 Mir et al PNAS 96 4262 4267 1999 3 Personal Communication Dr Georg Widera Genetronics Inc 1999 4 Salford et al Biochemical and Biophysical Research Communications 194 2 938 943 1993 5 Yamaguchi et al J Exp Med 172 291 293 1994 6 Nishi etal Cancer Research 56 1050 1055 1996 7 Muramatsu et al Biochemical and Biophysical Research Communications 233 45 49 1997 11 000 Specialty Products to Enhance Your Bioresearch Electroporation Electrodes CD Genepaddles Specifications Voltage Range 0 to 200 V DC Do not use AC current Pulse Length Range 1 to 99 msec in PBS Pulse Number Range 1 to 99 depending on voltage and pulse length settings Model 542 Paddle Dimensions 3x 5mm Paddle Configuration Rectangular Paddle Thickness 1mm Gold Plating Thickness 0 04 mm Model 543 Paddle Dimensions 3x 7mm The BTX Genepaddles are paddle style reusable electrodes suitable ie oa fia for a variety of applications Genepaddles come in two models each Gold Plating Thickness saan model consisting of a pair of electrodes The Model 542 Generator com patibility ECM 830 2001 and 630 Genepaddles feature rectangular 3 x 5 mm paddles while the Model 543 Genepaddles feature rectangular 5 x 7 mm paddles The models vary to suit the size of the target electroporation area These electrodes are connected to a Model 645 Square post Cable and Catalog No Model Product interfaced with a pu
22. F10ML MEW F12ML MEW F15ML MEW F20ML MEW F10FL MEW F12FL MEW F15FL MEW F20FL MEW F10T MEW F12T MEW F15T MEW F20T MEP F10A MEP F12A MEP F15A MEP F20A MEP F10B MEP F12B MEP F15B MEP F20B MEP F10ML MEP F12ML MEP F15ML MEP F20ML ASojolg 129 M41 www harvardapparatus com Electrode Holders ME Series Holders PE SERIES Holders Continued Glass OD Catalog No Wire Pellet Body Style Port Connector mm Model BS4 64 1188 female Luer 1 0 MEP F10FL BS4 64 1189 ez MEP F12FL BS4 64 1190 1 5 MEP F15FL BS4 64 1191 2 0 MEP F20FL BS4 64 1196 threaded MEP F10T BS4 64 1197 1 4 28 MEP F12T BS4 64 1198 MEP F15T BS4 64 1199 MEP F20T MP Series Holders Pe a ZAT Specifications Applications Ad R Body Material Acrylic d e Icroinjection sh Body Style Straight Port Ye barbed 1 tubing gt ID 2 barbed c m Pressure Ports tubing ID male Luer AA Say female Luer and 28 A Accepts 1 5 mm 1 16 tubing female threaded on B Accepts 2 4 mm 3 32 tubing Standard Lin ML Accepts female Luer fitting een pet noel FL Accepts male Luer fitting Handle Ae rae E T Accepts 1 4 28 male thread een ie ww fitting MP SERIES Holders a Paj Glass OD om Catalog No Body Style Port mm Model BS4 64 1261 Straight 1 16 barb MP S10A a gt B84 64 1262 MP S12A B54641263 MP S15A So BS4 64 1264 MP S20A BS
23. Interface Input Charge Time Voltage Range LV Mode HV Mode Pulse Length Range LV Mode LV Mode LV Mode HV Mode Internal self test upon start up Digital User Interface 110 V 220 V Universal 5 sec maximum without delay 5 to 500 V 1 V resolution 30 to 3000 V 5 V resolution 10 usec to 999 usec 1 usec resolution 1 msec to 999 msec 1 msec resolution 1 sec to 10 sec 0 1 sec resolution 10 to 600 usec 1 usec resolution Voltage dependent internally controlled Multiple Pulsing Pulse Interval Programmability Arc Control Safety Capacitance Amperage Dimensions H x W x D Weight Display Controls Monitoring Remote Operation Warranty 2 Years Catalog No Model Product BS4 45 0052 ECM830 ECM 830 Generator Only BS4 45 0002 8300 ECM 830 Electroporation System 1 to 99 100 msec 10 sec Storage for 2 setups V t n interval Arc Quenching Generator short circuit proof 4000 UF 500 A limit at 10 msec 14 x 31 8 x 31 1 cm 5 5 x 12 5 x 12 25 in 15 Ibs 6 8 kg 20 x 4 character LCD Single Rotary Encoder with push button toggle between all set parameters Additional on off Power and Start switches Monitoring and display of V t n interval Footswitch available Includes ECM 830 Generator BS4 45 0052 Cuvettes 1 mm 2 mm 4 mm pack of 30 10 each Cuvette Safety Stand BS4 45 0207 and Cuvette Rack BS4 45 0208 ASoj01g 129 Call for your local BTX Dealer or visit www btxonl
24. J Biol Chem 262 5476 5482 1987 2 Wilson D E Rumsey WL Green TJ and Vanderkooi JM 1988 The oxygen depend ence of mitochondrial oxidative phosphorylation measured by a new optical method for measuring oxygen J Biol Chem 263 2712 2718 3 Robiolio M Rumsey WR and Wilson D E 1989 Oxygen diffusion and mitochondr ial respiration in neuroblastoma cells Amer J Physiol 256 C1207 C1213 4 Shonat R D Wilson D E Riva C E and Pawlowski M 1992 Oxygen distribution in the retinal and choroidal vessels of the cat as measured by a new phosphorescence imaging method Applied Optics 31 3711 3718 5 Vinogradov S A and Wilson D E 1995 Metallotetrabenzoporphyrins New phospho rescent probes for oxygen measurements J Chem Soc Perkin Trans II 2 103 111 6 Vinogradov S A Lo L W Jenkins WT Evans S M Koch C and Wilson D E 1996 Non invasive imaging of the distribution of oxygen in tissue in vivo using near infrared phosphors Biophys J 70 1609 1617 7 Lo L W Koch C J and Wilson D E 1996 Calibration of oxygen dependent quench ing of the phosphorescence of Pd meso tetra 4 carboxyphenyl porphine a phosphor with general application for measuring oxygen concentration in biological systems Analy Biochem 236 153 160 8 Lo L W Vinogradov S A Koch C J and Wilson D E A new water soluble phosphor for oxygen measurements in vivo Adv Exptl Med Biol 428 651 65
25. M20P E45P F10N E45P F12N E45P F15N E45P F20N E45P F10P E45P F12P E45P F15P E45P F20P E45W M10N E45W M12N E45W M15N E45W M20N E45W M10P E45W M12P E45W M15P E45W M20P E45W F10N E45W F12N E45W F15N E45W F20N ASojo1g 12D M33 Electrode Holders E Series Holders E SERIES 45 Body Holders Continued Glass Catalog No Wire Pellet Connector OD mm Model BS4 64 0920 E45W F10P BS4 64 0921 E45W F12P BS4 64 0922 E45W F15P BS4 64 0923 E45W F20P Vented models are standard with the Warner OC 725 Oocte Clamp E SERIES 90 Body Holders Glass Catalog No Wire Pellet Connector OD mm Model BS4 64 0940 Pellet 2mm Pin 1 0 ERP M10N BS4 64 0941 1 2 ERP M12N BS4 64 0942 1 5 ERP M15N BS4 64 0943 ERP M20N BS4 64 0944 ERP M10P BS4 64 0945 ERP M12P BS4 64 0946 ERP M15P BS4 64 0947 7 ERP M20P BS4 64 0932 2mm Jack ERP F10N BS4 64 0933 ERP F12N eii BS4 64 0934 ERP F15N BS4 64 0935 l ERP F20N BS4 64 0936 Yes la ERP F10P BS4 64 0937 ERP F12P Eu BS4 64 0938 ERP F15P BS4 64 0939 ERP F20P BS4 64 0956 Wire 2 mm Pin ERW M10N E BS4 64 0957 ERW M12N BS4 64 0958 ERW M15N BS4 64 0959 ERW M20N BS4 64 0960 Yes la ERW M10P gu BS4 64 0961 ERW M12P BS4 64 0962 ERW M15P BS4 64 0963 ERW M20P BS4 64 0948 2 mm Jack ERW F10N BS4 64 0949 ERW F12N GWE BS4 64 0950 ERW F15N z BS4 64 0951 ERW F20N
26. MOS 96 200 Advanced Electroporation System includes ECM 830 Generator 2 x 96 Well Advanced EP Plates HT 200 Plate Handler and instructions MOS 96 100 Advanced Electroporation System includes ECM 830 Generator 2 x 96 Well Advanced EP Plates HT 100 Plate Handler and instructions 96 Well Disposable Electroporation Plate with clear bottom 250 ul capacity per well 4mm gap 25 Well Disposable Optimizer Plate with clear bottom 250 ul capacity per well 4mm gap Multi Well Plate Handler with Auto Sense Track Switching includes con nectors for standard BTX pulse gen erators Multi Well Plate Handler with Manual Track Switching includes connectors for standard BTX pulse generators ECM 830 Square Wave Electroporation System see pages M56 and M57 ECM 830 Generator Only ECM 630 Exponential Decay Wave Electroporation System see pages M58 and M59 ECM 630 Generator Only ASojo1g 129 MSS Electroporation Products Specialized Tools For Bioresearch M56 MOS 25 amp MOS 96 Plate Handlers Ce To complete the MOS 25 Systems there are two plate handlers avail able HT 200 and HT 100 The advanced HT 200 Plate Handler fea tures Auto track sensing which automatically switches columns in the Multi well plate during electroporation The Auto track sensing allows rapid electroporation of an entire plate by setting up the electrical parameters for all columns and pressing one button The HT 200
27. Other custom configuration of the leak free reference electrode are available please consult with Harvard Apparatus Technical Sales Department Low Leakage Reference Electrodes The low leakage reference electrodes are for use in applications where contamination would interfere with the application The electrode junc tions are resistant to most commonly used organic solvents They offer excellent resistance to acetonitrile DMSO THE MEK acetone dichloromethane esters alcohols and ketones Hydrofluoric acid or common dilute acids and bases have no effect on the on these electrodes Two low leakage reference electrodes are available One is 2 mm 0 08 in in diameter and the second is 5 mm 0 2 in in diameter Other cus tom configurations of the low leakage reference electrodes are available please call the Harvard Apparatus Technical Sales Department LEAK FREE Reference Electrodes 1 mm 0 04 in 2 mm 0 08 in 100 mm 3 9 in 65 mm 2 6 in PEEK PEEK 0 0 3 M KCI 3 M KCI 1 mm gold plated pin 1 mm gold plated pin lt 10K QF lt 10K QT 5 to 80 C 5 to 80 C Distilled water Distilled water BS4 69 0053 BS4 69 0023 This miniature flexible reference electrode is 3 inches long and has an outside diameter of 2 mm It comes complete with a 4 foot long cable and is terminated with an alligator clip connector Each unit comes pre filled with the reference solution 3 0 M NaCl in 25 Glycerol and it is stored dry If re
28. PH Anal 32 BIT Factory Calibration SVC for FOXY Sensors Fiber Optic Oxygen Probe Polyimide Probe 600 um core D Fiber Optic Oxygen Probe Aluminum Probe 500 um OD Fiber Optic Oxygen Probe Rugged 1 um D Silica Core Fiber in 0 25 in OD Stainless Steel Ferrule with Screw On Light Shield Bifurcated Optical Fiber Assembly 200 um Bifurcated Optical Fiber Assembly 400 um Harvard Apparatus owns Hugo Sachs Elektronik The HSE isolated organ baths set the industry standard They can be found in the Isolated Organ and Tissue Section K 11 000 Specialty Products to Enhance Your Bioresearch Dissolved O LICOX McB Universal Oxygen and Temperature Monitor e Used to measure oxygen partial pressure pO in gases fluids suspensions and biological media e Determination of saturation or pressure of dissolved oxygen in fluid e Small battery operated and water resistant e deal for Cell culture media for controlling microbial growth conditions in science and pharmaceutical industry Living tissue and body fluids for biomedical research and veterinary medicine Plants fruits and meat for food science and technology Mud silt and soil for toxicological and environmental monitoring The LICOX MCB is made for oxygen measurements with the flexible REVOXODE catheter micro probes The temperature is compensated by thermocouple microprobes that have a built in flexible polyethylene or metal tube for more
29. Pumps Section A Product Screw Actuated Micrometer Driven Hamilton Syringe Catalog No BS4 69 0107 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Micro Incubation Specialized Tools For Bioresearch Heating of Imaging and Recording Chambers Application Fundamentals Why Temperature Control Researchers have long understood the importance of temperature regulation in the study of cellular function For example a Medline search using the keywords temperature and cell produced over 18 000 references since 2003 most addressing the effect of tempera ture on the biophysical and metabolic function of cells In addition the performance of imaging system optics are exquisitely sensitive to temperature as revealed by a shifting focal plane as the temperature of the objective is varied Therefore for the scientist studying the func tion of cells in a recording environment the importance of tight ther mal control cannot be overstated A number of issues confront the researcher attempting to maintain temperature control These include maintaining a constant and uniform bath temperature under various flow rates and controlling for changes in the ambient temperature of the environment In addition effects due to the addi tion of cold drugs or solution to the perfusion path and heat sinks due to microscope optics and stage adapters must be addressed General Issues Sources of Hea
30. adapters are also available call our technical support department for details See page M114 for Series 20 Stage Adapters Catalog No Model Product BS4 64 0349 DH 35i Culture Dish Incubation System DH 35i Accessories Replacement Parts BS4 64 0351 AR 4 Replacement Adapter Ring Kit for DH 35i set of 4 BS4 64 0100 TC 324B Heater Controller Single Channel BS4 64 0101 TC 344B Heater Controller Dual Channel BS4 64 0106 CC 28 Cable Assembly for Heater Platforms BS4 64 0102 SH 27B Solution Heater 11 000 Specialty Products to Enhance Your Bioresearch Chambers for 35mm Cell Culture Dishes RC 37 Cell Culture Dish Perfusion Chamber Insert Aspirator Agar Bridge Well gt Perfusion Inlet Port Culture Dish Perfusion TOP VIEW Designed for glass bottom 35 mm culture dishes Permits imaging recording and perfusion in the culture dish Open and closed bath designs Incorporates Warner s diamond fluidics Ideal for rapid screening assays The introduction of thin glass bot tom culture dishes has expanded the use of these popular tools dramatically Applications such as live cell imaging confocal microscopy and time lapsed pho tography are now possible The ability to exchange the extra cel lular environment by perfusion within 35 mm dishes has been difficult at best until now The RC 37 Perfusion Chamber Insert for cell culture dishes makes perfusion within these dishes a sim
31. amiNO 2000 amiNO 600 Note Room Temperature Sensitivities The above selection guide is based on design sensitivity and economy For example amiNO FLAT and amiNO IV are the most sensitive but are also the most expensive The amiNO 600 is less sensitive but is more economical U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M3 Nitric Oxide E Specialized Tools For Bioresearch M4 C inNO T Nitric Oxide Measurement System Current Scale 2 500 000 0 pA BS4 69 0013 Electrolyte Concentration Scale 1 000 000 0 nM BS4 69 0014 Replacement Membranes for BS4 69 0012 Resolution 0 01 nM 0 1 pA pkg of 4 Screen Meter 7 5 digit meter BS4 69 0016 SensoReady Electrode Polarizer Recording Speed 1 to 1000 sec div BS4 69 0029 Adapter LIMO Connector to amiNO Sensor Power Replaceable battery Connector Dimensions HxWxD 76 x 229 x 203 mm 3 x 9 x 8 in Note inNO and inNO T are battery operated Voltage selection is for Magnetic Stirrer Call Harvard Apparatus Customer Service for Nitrate Reduction Products Temperature Range 0 to 50 C inNO T only Temperature Resolution 0 01 C inNO T only Temperature Sensor lt 0 50 mm tip 120 mm long inNO T only amiNO Sensor Performance Comparison and Ordering Information Catalog No Quantity Model Size Typical Sensitivity Competitor Typical Sensitivity BS4 69 0008 2 amiNO 7 7 um 0 5 3 pA nM typical 1 5 pA nM n a BS4 69 0009 2 amiNO 30 30 um 2
32. an electrode is produced manually using the start stop key All further electrodes are then prepared automatically according to the manual setting by pressing the repeat key The end of the chloridation process is indicated by a beep and the current source is automatically switched off The counter electrode cathode used is a carbon glass ceramic crucible This material has a very high electrical conductivity is very largely inert physiologically and resistant to oxidation and corrosion There is therefore no contamination of the AgCI film through free metal ions as is the case when using stainless steel as a cathode for example Chloridation Current Electronically controlled constant current source with current monitoring facility and with low medium high selection by switch corresponding to approx 5 10 15 mA respectively Chloridation Voltage Max driving voltage of constant current source is approx 15 V and is therefore completely safe for user Start Stop Manual operating mode start stop for chloridation of single electrodes Repeat Automatic repetition of manually set chloridation procedure Dimensions HxWxD 15x6x 12 cm 5 9 x 2 4 x 4 7 in Weight 750 g 1 7 Ib Catalog No Product BS4 73 0004 HSE Auto Chloridizer Model 863 115 VAC 60 Hz BS4 73 0005 HSE Auto Chloridizer Model 863 230 VAC 50 Hz BS4 73 0006 Spare Crocodile Clips BS4 73 0007 Maintenance Kit for Silver Electrodes Emery Paper and Scotch Brite Cleaning Sponge
33. are usually calibrated within humid chamber in protection cover which is delivered with sterile probe Cover is inserted into BS4 69 0188 calibration device This is not required for BS4 69 0201 REVOXODE because temperature probe can be directly inserted sterile into oxygen probe U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M11 Dissolved O E Specialized Tools For Bioresearch M12 LICOX MCB Universal Oxygen and Temperature Monitor Classification Principle Probe Input Display Oxygen Range Temperature Analog Signal Output Oxygen Temperature Calibration Accuracy and Reproducibility Oxygen Channel Temperature Channel Power Supply Case Dimensions HxWxD Case Weight Net Lab equipment C electrical safety meets IEC 61010 1 EMI 61000 4 part 2 to 6 EN 50204 EME EN 55011 Polarographic oxygen probe circuit with temperature compensation and type K thermocouple amplifier 0 to 10 000 nA oxygen 1 to 8 mV temperature both circuits isolation amplifiers breakdown voltage gt 1 5 kV 1 0 to 199 9 mmHg kPa or 2 0 to 1999 mmHg hPa or 199 9 to 199 9 useful range 40 C to 199 C 0 to 5 V 5 mV display unit 0 4 to 2 V 10 mV C Adjustment of amplifier gain to sensitivity of REVOXODE when placed in humid chamber of known temperature and known or reference oxygen content offset calibration not required for REVOXODE calibrati
34. dishes LU CSD MSC TD or MSC PTD 0 5 to 2 0 L min Ambient to 50 C when used with TC 202A 0 2 C when used with TC 202A 0 3 C gas flow of 1 5 L min 3 5 ml media volume Recommended Gas Flow Temperature Range Temperature Stability Temperature Gradient Across Chamber Heating Element 2 0 Q Nichrome Coil Dimensions H x D 16 x 90 mm 0 63 x 3 54 in overall Weight 150 g 5 3 oz Microscope Stage Mounting Call Harvard Apparatus Inc for options Catalog No Product BS4 65 0040 LU CB1 Leiden Micro Incubator with BSC TH Tissue Pins and 3 Alignment Rings Nikon Olympus and Zeiss and Output Cable BS4 65 0045 TC 202A Bipolar Temperature Controller BS4 65 0100 Output Cable TC 202A to Microincubator For pH Controller see page M144 M97 Special Chambers Systems M98 LU CPC CEH Leiden Closed Perfusion Micro Incubator BS4 65 0041 LU CPC CEH Micro Incubator This micro incubator features a short optical path between coverslips to allow single cell flu orescence measurements under maximum objective magnifica tion using either an inverted or upright microscope Media access is flexible Four symmetri cally placed ports are provided with plugs which are designed so that direct streaming of perfusate onto the cells is avoided Plug rotation creates different degrees of mixing There are four additional ports for inserting special purpose probes e g temperature pO or hypodermic needles to introd
35. dishes The platform is at home on both upright and invert ed microscopes and includes resistive heating elements The base has a 25 mm diameter aper ture Magnetic stainless steel along the perimeter of the QE 1 permits the use of Warner s MC 1 Magnetic Clamp Kit which includes a variety of holding tools Removable perfusion and suction tubes allow perfusion in any of the RC 40 Imaging Chambers or 35 mm dishes Suction and perfusion tubes also feature set screw locks for the horizontal location and adjustable presets on the vertical for setting the bath height These QE 1 with Serfes 40 Chamber adjustments permit easy and exact positioning and repositioning of and Stage Adapter the suction and perfusion tubes A redesigned suction tube elimi nates tidal action and fluid flutter making aspiration very quiet A Resistive heating of the QE 1 is provided by means of our Perfusion Q P y Tube TC 324B TC 344B Temperature Controllers and a CC 28 Cable A a a Heating of perfusate is achieved using our SH 27B or SF 28 In Line Solution Heaters Adapter rings for Corning Falcon and Willco 35 mm dishes are sup plied to ensure a close fit in the QE 1 Stage adapters are available for all major microscopes Custom or modified stage adapters are also available call our Technical Support Department for details Catalog No Model Product Ag D BS4 64 0375 QE 1 Quick Exchange Heated Base ements w Perfusion and Adapter Ring K
36. easy insertion or replacement of the platinum iridium or other filament materi al forge element The vertical and horizontal position of the heating element can be precise ly controlled by the two controls mounted on the operating head and main electrode casting Two controls on the stage are used to position the vertical and horizontal movement of the microtool The electrode holder can be positioned either vertically or horizontally and can easily be replaced The electrode holder and heating fila ment can be pivoted as a unit horizontally in a short arc causing the electrode to move paral lel to the optical axis about 1 2 inch Two curved air jets are also mounted on the adjustable hold er system and are equipped with an adjustable valve These jets direct airflow to the wire heat ing filament to provide spot cooling of the heat ed element Adjusting the current to the elec trode controls filament heat The heat at a given setting will vary depending on the size and type of filament wire the amount of air blown against the filament and the amount of heat conducted away from the filament by the tool The system is supplied with platinum iridium wire for the electrode and has the ability to uti lize other types and gauges of forging fila ments The apertures of the electrode pins will accommodate up to 22 gauge 0 0253 inch diameter filament wires The following items are also included with the system 4 microtool rea
37. for Cleaning Silver Electrode before Chloridation Microelectrode Starter Kit This starter kit makes entry into microelectrode production technology easy for researchers who have not yet produced any microelectrodes themselves It contains all the neces sary aids components and consumables A puller must be purchased separately see page M52 In addition to manual dexterity which is always necessary the user requires a binocular transmitted light microscope with eyepiece eraticule and up to 500X magnification for optical checking of the tip diameter The kit is suitable for producing intracellular microelec trodes using borosilicate glass capillaries with an integral filament see pages M45 to M49 The filament makes micro electrode filling easy Special filling devices such as vacuum filling equipment are therefore not required In addition to a transport and storage container for completed microelec trodes the kit also includes a specially developed electrolyt ic chloridation unit for producing an AgCl film on silver elec trodes see to the left Controlled application of the AgCl film through the selection of current density and chloridation period in the case of the electrolytic chloridation method together with the good reproducibility of the AgCl film read ily justify the increased cost of purchasing the electrical chloridation equipment By contrast chemical chloridation involves less equipment but produces poorer chlor
38. in one compact battery powered unit When used with the amiNO series of NO sensors this unit provides an NO detection sensitivity increase of up to 100 times that of any other electrochemical detection system on the market today lts state of the art bat lery powered design allows the unit to run continuously for up to 5 years making it ideal for any electrically isolated and or portable application The inNO T system is the first available nitric oxide system to include tempera ture compensation which further enhances the detection limits and data reliability by virtually eliminating base line drift caused by temperature changes A temperature probe is supplied with each system Its internal 24 bit ana log to digital converter and built in dig ital notch filtering allows for accurate noise free detection of Nitric Oxide levels of less than 0 01 nM The 24 bit A D tech nology also gives the unit a dynamic range in excess of 16 000 000 to 1 Its sophisti cated control and analysis software can be linked to any computer system that utilizes Windows 95 98 2000 or NT via the inNO s internal RS 232 interface amiNO Series of Nitric Oxide Sensors e All NO sensors are combination sensors no external reference electrode required e Mechanically rugged membrane e All sensors covered with proprietary triplecoat gas permeable membrane to guarantee selectivity and very fast response time e All
39. incubated isolated cardiomyocytes in Oxystat at available oxygen concentration of 90 mm Hg Curve 1 added vol ume Curve 2 chamber pO Arrows Instants of sampling for biochemical analysis Typical Recording Fig 3 shows an original recording Isolated cardiomyocytes were incu bated at a pO of 90 mm Hg and seven samples were withdrawn con secutively for biochemical analysis Curve 2 is the record of the pO electrode The electrode was calibrated with aerated solution 155 mm Hg After the cells were added the pO in the chamber drops steeply When the pO falls below 90 mm Hg the controller starts the pump and aerated solution is added curve 1 until the pO has returned to its set value The pump is operated more or less frequent ly depending on the O consumption of the cells From the solution volume added in unit time i e the slope of curve 1 it is possible to calculate the oxygen consumption of the isolated cardiomyocytes After taking the samples 0 8 ml each arrows the total quantity of cells in the chamber is reduced and less solution is required in order to main tain the chamber pO at 90 mm Hg The slope of curve 1 is corre spondingly reduced Typical Application The relationship between available oxygen and oxygen consumption of isolated cardiomyocytes has been investigated under different forms of stimulation Fig 4 Resting cardiomyocytes triangles have a constant oxygen consumption over a wide pO range 1 to
40. is ideal for a busy research laboratory running many daily samples The Model HT 100 requires manual switching from column to col umn and is ideal for laboratories doing basic electroporation research or fewer samples Both accept either the 96 Well or 25 Well Optimization Plates and provide a safe and reliable working area for processing the Electroporation Plates Both Plate Handlers have pulse switching technology integrated into the package and gold plated contacts to mate with the Electroporation Plate The Plate Handlers were designed to operate in space constrained areas such as laminar flow hoods Catalog No Model Description BS4 45 0401 HT 200 Multi Well Plate Handler with Auto Sense Track Switching includes con nectors for standard BTX pulse gen erators BS4 45 0400 HT 100 Multi Well Plate Handler with Manual Track Switching includes connectors for standard BTX pulse generators Call for your local BTX Dealer or visit wwwbtxonline com Gi ECM 830 Square Wave Electroporation System e Superior square wave technology and bipolar pulsing lead to higher viability and efficiency e Low voltage mode 1 V discrimination and 0 5 accuracy allows for greater reproducibility for low voltage applications such as In Vivo gene therapy In Ovo gene transfer and nuclear transfer applications e Monitoring of voltage pulse duration pulse interval and pulse number allows for optimization of protocols trou bleshootin
41. leads are approximately 16 cm long and terminated with 1 mm diameter pins The RC 27N and RC 27NE chambers use a 24 x 50 mm coverslip on the bottom Both models are used with either P 6 or PH 6 heater platforms see page M112 RC 27N and RC 27NE Material Polycarbonate Bath Dimensions Length x Width 37 x 8 mm Specimen Area 28 x 8 mm Volume mm of Solution Height 305 ul per mm of solution PE 160 Tubing 10 ft 24 x 50 mm 1 Coverslip pkg of 50 P 6 Plain Platform PH 6 Heating Platform Standard Accessories Supplied Platforms Used With Product Narrow Bath Recording Chamber Catalog No Model BS4 64 0239 RC 27N BS4 64 0240 RC 27NE Narrow Bath Recording Chamber with Field Stimulation Note RC 27N and RC 27NE Chambers used with either P 6 or PH 6 Heater Platforms and Stage Adapter see pages M112 to M115 For our complete line of Platforms and Adapters see pages M112 to M115 Slice Hold Downs available in Kits see page M117 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M107 Special Chambers Systems Large Bath Chamber RC 27L Specialized Tools For Bioresearch M108 Bath Area 17 5 mm Suction Well Perfusion Inlet Perfusion Spreader Volume 560 ul SIDE VIEW Suction Tube Slot a 3mm ES i E A 4 mm P Volume is for 1mm solution For a complete listing of individual Slice Hold Downs and kits see pages M116 and M1
42. mm 3mm 0 to 60 C BS4 69 0071 Spintrode P 180 mm 3mm 0 to 60 C BS4 69 0072 Freezetrode 120 mm 12 mm 20 C to 80 C BS4 69 0073 Flushtrode P 120 mm 12 mm 10 to 50 C BS4 69 0074 Tiptrode 25 mm 6 mm 0 to 80 C Minitrode P The small size of this sensor makes it ideal for pH measurement of sample in microplates and microcentrifuge tubes The sensor tip is 3 mm diameter with a usable length of 100 mm 3 9 inches Freezetrode This sensor is ideal for measuring the pH of samples stored in cold rooms Select this sensor if your sample is between 20 to 80 C It has a 12 mm diameter tip Flushtrode P If your sample contains between 5 to 85 water this is the ideal sen sor for you It is good for determining pH of buffer solutions It has a 12 mm diameter tip Tiptrode This pH sensor was designed for pH measurements in applications requiring penetration of solid and semi solid materials including foods gelatinous materials and viscous buffers It has a 6 mm tip Spintrode P Use the SPINTRODE to determine the pH of samples in NMR tubes The 3 mm diameter 180 mm 7 inch long sensor allows you to deter mine the pH without transferring the sample from the NMR tube These pH Electrodes are supplied individually and have S7 type connec tors To ensure the most accurate pH measurements calibrate your electrodes with DURACAL Buffer Solutions see next page for more information
43. oe Q Coaxial Cable The Genetrodes may be positioned in parallel at a prede nesses A a C termined gap within a tissue using the Model 515 Genetrode Holder j gr Generator Compatibility ECM 830 2001 and 630 General Applications Genetrode Holder Interelectrode Gaps 1 to 10 mm in 1 mm increments vo Genetrodes may be used for many in vivo and in ovo electroporation O applications including drug and gene delivery The electrodes are Catalog No Model Product l om placed into a tissue following injection of the molecule of interest and 854 45 0113 508 Straight Electrode 5 mm Gold Tip eS an electroporation pulse is delivered using a BTX Flectroporation BS4 45 0114 510 Straight Electrode 10 mm Gold Tip k System such as the ECM 830 2001 and 630 The electric field intro BS4 45 0115 512 Bent L shaped Electrode 5 mm Gold Tip 2 duced by the Genetrodes causes transient pores to form in the cells of BS4 45 0116 514 Bent L shaped Electrode 3 mm Gold Tip m the tissue allowing uptake of the molecules into the cells Genetrodes gs4 45 0117 516 Bent L shaped Electrode 1 mm Gold Tip ae may be cleaned with a mild detergent and sterilized with ethanol or eth BSA 45 0203 515 Genetrode Holder ylene oxide Properly maintained Genetrodes have a life span of approx l BS4 45 0201 465 Square Post Micrograbber Cables m imately 1500 pulses and are compatible with most BTX Electroporation Systems BS4 45 0210 5343 Banana Coaxial Connection Cable
44. of LICOX for experimental biomedical research in brain cerebrospinal fluid muscle connective lissue myocardium tumors gut liver and other internal organs please contact Harvard Apparatus Customer Service 11 000 Specialty Products to Enhance Your Bioresearch a BS4 61 01 eel Dual Chamber Oxygen Measuring System The BS4 61 0130 is a complete flexible sys tem designed for oxygen measurement in small samples as is frequently required in biological research The system is designed around our miniature Clark style polaro eraphic electrode Measuring only 1 8 of an inch in diameter 3 2 mm it is ideal for metabolic studies of mitochondria cell sus pensions and enzyme assays This system includes everything you need for oxygen measurement on two independent channels a dual channel amplifier two oxygen elec trodes and two chamber systems It also includes accessories to simplify electrode maintenance The chambers can be set up in two basic configurations Flow Cell Mode For in line measurement of oxygen in solu tions flowing past the electrode This set up is often used to measure oxygen uptake of per fused tissues by measuring inflow pO with one channel and comparing it with the out flow pO as measured by the second channel Batch Cell Mode For measuring oxygen consumption in a sealed 600 pl sample The batch cell chamber includes a magnetically coupled stirring sys tem connections for circulating wate
45. on chamber and contains stirrer with motor and upper stimulation electrode U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M15 Respirometry _ Specialized Tools For Bioresearch M16 HSE HA Oxystat System Type 1000 Cannula for Adding Stirrer Air Saturated Solution Stimulation Electrode Platinum Water Jacket 37 Oxygen Electrode F lt Sampling Mi 4 Fiber Optics Filter Electrical Screen Stimulation Electrode Platinum Outlet Figure 1 Oxystat Chamber Oxystat System Components The Oxystat 1000 System includes the following components e Oxystat Incubation Chamber with Stirrer BS4 73 0117 e Power Supply for Stirrer BS4 73 0118 or BS4 73 0119 e pO Electrode BS4 73 0120 e Control Unit Model 719 with amplifier and Pump BS4 73 0122 e Stimulator BS4 73 0124 e Thermocirculator BS4 50 1932 or BS4 50 1940 All of these components must be purchased separately In addition to the above equipment a chart recorder or data acquisition will permit recording of data for further analysis The Oxystat chamber is an incu bation chamber with a volume of 10 ml Figure 1 Mechanical dam age to the cells during stirring of cell suspensions was an important disadvantage of previous systems special attention has therefore been paid here to a very gentle stirring action The cells are introduced into the chamber and the chamber is filled w
46. our technical support staff and we will be glad to aid you in determining the best application of these heating strategies to your experimental setup U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 2D M81 Micro Incubation The Series 40 Family of Imaging Chambers M82 i N he a k 7 o CS i i E E y np a mT A i e ae 4 I i ey f s r T S bA a e one BA 2 gt e w ON A s A E IC i 4 0 tO T ure arrow Bath bS w x P i e lt eee ee se a RC 40 Quick Change Imaging Chambers Warner Instruments is pleased to introduce a new family of cham bers designed to meet the need for rapid screening of cells grown on coverslips The innova tive design uses O rings to seal the coverslip to the polycarbon ate chamber Therefore little or no silicone grease is needed and mechanical fasteners are not required for coverslip mounting Chambers are available for use with 15 18 and 25 mm round coverslips Round low profile chambers maximize the viewing area and provide optimal access for electrodes Slotted bath chambers allow rapid solution exchange A version which includes platinum wires may be used to study cardiac myocytes and other applications requiring field stimulation The RC 40HP High Profile Chamber allows for a deep bath The Series 40 chambers are designed to be compatible
47. pipette There is no need for any manual rota tion or any inconsistent timing interrupt control Exclusive Optical Digital Taper Measurement Instead of a mechanical tip length setting as on other brands of pipette pullers the PMP 102 and PMP 107 use an exclusive optical digital ruler to perform precise taper length setting real time measuring and controlling With this feature a user can handle the taper pulling of pipette tips precisely and very easily Equipped with a powerful computerized tip sensing function the PMP 102 and PMP 107 can automatically finish the tip pulling BS4 69 0150 CE Computerized Heater Control The PMP 102 and PMP 107 use an advanced microcontroller design to perform real time heater monitoring and pulling control Thus they will always provide precise heating power despite multiple consecutive pipette pullings or changes in thermal electrical characteristics Programmable Sequences for Creation and Reproduction There are 25 user programmable pulling sequences with 18 steps in each sequence Users can start the pulling sequence by just pressing the Start button Factory Installed Preset Programs for Major Different Pipette Pulls Every pipette puller is pre installed with a number of different pipette pulling programs With just a few modifications to these steps or parameters new programs can be configured Pneumatic Pulling Force Both pullers use a precise controlled pneu matic pressur
48. replaced The electrode is stored wet Response Time 10 sec Slope 55 mV per pH range of 1 to 14 Impedance 200M Q Stability 0 05 pH per day Dimensions L x D 31 7 cm x 3 mm 1 25 x 0 1 in Product Combination pH Electrode in 11 ga SS Tube Catalog No BS4 69 0497 Combination Gastroesophageal pH Electrode Luer Cap LE Reference Electrolyte pH Electrode Plastic Holder 1 6 mm Flex Tube 1 i Coax Cable 4 ft Reference Electrode Tee Fitting This miniature combination pH reference electrode has a long 3 foot long flexible tube to fit into the esophagus It does not require an external reference electrode The reference solution can be replaced The electrode is stored wet Response Time 10 sec Slope 55 mV per pH range of 1 to 14 Impedance 200 M Q Stability 0 05 pH per day Electrode OD 1 6 mm Dimensions L x D 91 4 cm x 1 6 mm 3 ft x 0 06 in Product Combination Gastroesophageal pH Electrode Catalog No BS4 69 0495 Flexible Combination pH Electrode for Free Flap Surgery Luer Cap L Reference Electrolyte Plastic Holder pH Electrode 1 6 mm Flex Tube 1 m long Coax Cable 4 ft Tee Fitting Reference Electrode This miniature combination pH reference electrode has been specifi cally designed to be used in free flap surgery It does not require an external reference electrode The reference solution can be replaced The electrode is stored wet Response T
49. report page The results may be exported to a spreadsheet word processing or statistics package The Analysis screen and the Report page may be printed if required tie Alfozan Cytotoxicity 2 Wed Jul 19 2000 ime 16 21 03 37 0 C Comments Effects of 1 244 ppm Alfozan added cumulatively on BG625 02 Filename C Strathkidata Ccelldem dat Biomass mg Vo frat Chamber A 0 79 24 Chamber B 0 83 24 Chamber C 081 24 Chamber D 0 89 24 Chamber A Chamber B Horm Rate Horm Rate moki mg umoth my 27 837 26 251 22 969 21 811 16 161 15 78 Start Length hhmmiss hiimmss 1 No teatment 2 1ppm Alfozan 3 2ppm Alfozan 0 00 41 0 03 12 0 05 35 0 02 06 0 02 02 0 02 31 When the software is used for dissolved oxygen monitoring the Analysis screen which displays the recorded traces and the oxygen values at flagged points or at set times can be transferred to the Results and report page The program requires a computer with a minimum specification of Pentium processor 1 free serial port 64 Mb RAM Windows 95 or later and 4 MB hard disk space A comprehensive Instruction Manual is supplied which will be particularly valuable to anyone who is not familiar with respirometric work This software is supplied with the 6 Channel Oxygen System Model 928 and the 1 2 Channel Oxygen Meter It is not available separately U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 2D
50. than one year of use Every probe is delivered sterile and can be easily calibrated within its sterile protection cover For measurements with LICOX MCB a REVOXODE oxygen probe and a type K thermocouple are simultaneously used Temperature and oxygen are displayed on LCD illuminated in the dark the analog outputs can be used for recording the data with the data collection system LICOX DAQ or a paper chart recorder The REVOXODE probe is a new electro chemically reversible type of a polarographic microprobe It has a built in flexible polyethylene tube allowing for over 120 hours of continuous use The probe is remarkably accurate in long term use in particular at low oxygen levels In the oxygen circuit of LICOX MCB the temperature is specifical ly compensated for REVOXODE probes The oxygen display of LICOX MCB is calibrated by ampli fier gain adjustment while the probes are exposed to the reference atmosphere or fluid Thus the display can be adjusted to absolute pO values in mmHg or hPa if the pO of the calibration chamber is calculated from a formula given in the instruction manual or to relative values where the oxygenation in the calibration chamber is set as 100 The LICOX MCB system includes BS4 69 0189 and BS4 69 0190 probe cables BS4 69 0197 and BS4 69 0198 out put cables BS4 69 0185 BS4 69 0186 or BS4 69 0187 power supply BS4 69 0183 rechargeable batteries and BS4 69 0201 probe ASojolg 129 Probes
51. the constant temperature bath containing the respirometer chamber Specifications Material Electrode Holder Black acetal Collar Black acetal Chamber Clear acrylic Inlet Outlet 6 mm OD and 3 mm ID stainless steel Catalog No Model Product BS4 69 3023 FC 100 Flow Cell i General Electrode Holder EH 100 E e For use with any of your own custom respiration chamber designs The electrode holder is a black acetal tube in which the electrode is located so that its tip projects from the end The holder is used for the insertion of the electrode into your own respiration chamber An acrylic collar containing an O ring on its inside surface is also provided This can be cemented over a 13mm hole drilled in the wall of the respiration chamber to facilitate insertion of the electrode holder The electrode should only be used in an electrode holder to avoid ground leak damage O Electrode Holder Acrylic Collar Dimensions 13 x 85 mm D x L 26 x 10 mm D x L Holder Material Black acetal Clear acetal Catalog No Model Product BS4 69 3024 EH 100 Electrode Holder 11 000 Specialty Products to Enhance Your Bioresearch pH and Reference Electrodes Microminiature CO and pH Electrodes This series of micro pH 0 and CO electrodes has been specifically designed for use in various area of bioscience research Their micro miniature design and small sensing area allows them to be used for small area measurements This series of
52. the platform using either a Peltier or liquid based heating element Regardless of the method employed this strategy is best suited for conditions where the solution flow rate is extremely slow or nonexistent A number of considerations are important when using this approach First the perfusing solution receives heat energy only after entering the chamber thus generating a temperature gradi ent within the bath Second since heat energy is only applied to the edges of the chamber by the platform the rate of heat transfer to the solution is very slow However this approach excels for condi tions where solution flow is nonexistent such as maintaining a constant temperature while loading cells with indicator dyes Solution Reservoir Heating Heating the solution reservoir is not generally considered a success Jul strategy for maintaining temperature in the bath since most if not all of the heat energy is lost en route to the chamber However this technique is important if outgassing of solutions is a problem Since the gas load of a solution is dependent on partial pressure and tem perature preheating the solution at atmospheric pressure before delivery to the final heater will minimize the occurrence of bubbles in the bath even if the solution is allowed to cool en route Therefore solution reservoir heating ts best used in combination with other heat ing techniques as described below Microscope Objective Heating While nece
53. thickness are also available The bath has a large viewing area with an aperture of 17 7 mm D The chamber bottom is beveled to permit maximum access to the bottom coverslip The chamber is reversible and may be mounted in the stage adapter to accommodate both upright and inverted microscopes The RC 30HV is an optional heater version which includes a pair of resistive heaters mounted to the chamber base Heaters are compatible with Warner TC324B TC344B Heater Controllers see page M126 These Confocal Imaging Chambers are supplied with six pre cut 250 ym thick gaskets two large baths 133 pl volume two slotted baths 25 6 pl volume two blank for customized coverslips 22 x 30 mm 1 and 22 x 40 mm 1 pkg of 50 each and PE 90 Tubing 3 3 m Physical Dimensions 9 5 x 82 mm D Weight lt 90 g assembled with optional heaters Base and Pressure Plate Aluminum black anodized Top Coverslip Plate Polycarbonate Gaskets Silicone Rubber Input Output Tubing PE 90 Coverslips 1 Top 22 x 30 mm Bottom 22 x 40 mm Catalog No Model Product BS4 64 0320 RC 30 Confocal Imaging Chamber BS4 64 0321 RC 30HV Confocal Imaging Chamber with Heaters Optional Accessories BS4 64 0322 SA 30NIK Stage Adapters for Nikon Diaphot TE200 TE300 BS4 64 0323 SA 30TMS8 Stage Adapters for Nikon TMS with 8 x 12 cm Cutout BS4 64 0324 SA 30TMS9 Stage Adapters for Nikon TMS with 9 x 13 cm Cutout BS4 64 0325 SA 30 OLY2 Stage Adapters for Olympus
54. to Enhance Your Bioresearch Electroporation Products cap ECM 2001 SLECTAD CELL WAMULATON ECM 2001 The ECM 2001 is a versatile electro cell manipulation instru ment It generates a proprietary AC wave form for benign dielec trophoretic alignment of cells With only microsecond switch over time from AC to DC effi cient fusion can take place After fusion AC reapplication keeps cells together for the rounding off process The DC square pulse section can be used as a stand alone powerful electroporation system with a wide range of voltages and pulse lengths capable of operating into low impedance loads The 1 MHZ AC pulse aligns cells in real time and saves time faster in comparison to PEG Electrofusion process takes seconds PEG takes hours Researchers can change parameters voltage or time to get the maximum number of hybrids aligned in real time This helps in the alignment of blastomere to oocyte at the correct position for better fusion accuracy for embryo cloning The researcher is able to watch the fusion process under microscope which makes opti mization much easier The 3000 Volt DC square wave pulse delivers a high voltage square wave electroporation pulse All 800 Series protocols can be used with this system The researcher will own two complete systems electroporation and electrofusion in one making the ECM 2001 more versatile A pulse length of 1 99 psec allows for an extensive range of
55. 0 g each and 10 cat cats weighing 3 kg each experiments using the PMOD instrument Lyophilized porphyrin contains approx 9 Pd meso tetra 4 carboxyphenyl porphine which is bound to 80 BSA clinical grade low fatty acid 8 NaCl and 3 TRISMA buffer Accessories include one 1 10 cc syringe three 3 syringe filters 25 x 22 micron and three 3 4 0 mL amber glass vials with foil caps Pd meso tetra 4 carboxyphenyl porphyrin dendrimer Oxyphor R2 This new water soluble phosphor has significant advan tages over the parent compound for experiments in vivo The quench ing properties are similar to those of the parent compound with respect to the effect of albumin However because it is highly water soluble it can be injected into the blood as a simple solution in phys iological saline The pH dependence of Oxyphor R2 is also very small over the physiological range see Lo et al 1997 Oxyphor R2 is largely excreted in the urine in mice and other animals In mice excretion occurs with a half time of less than 2 hours Pd tetra 4 carboxyphenyl tetrabenzoporphyrin dendrimer Oxyphor G2 This is a water soluble phosphor that has an absorption band near 636 nm and emission near 800 nm and can be used for O measurements in tissue up to 3 cm thick U S Toll Free 800 272 2775 e Fax 508 429 5732 Online www harvardapparatus com ASoj01g 29 M7 Dissolved O Specialized Tools For Bioresearch Phosphor
56. 000 Specialty Products to Enhance Your Bioresearch Electrode Holders ME Series Holders Body Material 9 3 mm diameter acrylic Body Style Straight Connector 2 mm jack MEW FxxT Coupling Ag AgCl pellet or Ag Wire Pressure Ports Standard Glass Sizes 1 0 1 2 1 5 and A Accepts 1 5 mm 1 16 tubing 2 0 mm Handle 6 3 mm diameter B Accepts 2 4 mm 3 32 tubing ML Accepts female Luer fitting x 6 3 cm long threaded to attach to holder MEW FxxFL FL Accepts male Luer fitting T Accepts 1 4 28 male thread fitting Holders with the T port are compatible with the Picospritzer system made by General Valve Corp PE SERIES Holders Glass OD Connector mm Model Catalog No BS4 64 1220 BS4 64 1221 BS4 64 1222 BS4 64 1223 BS4 64 1224 BS4 64 1225 BS4 64 1226 BS4 64 1227 BS4 64 1232 BS4 64 1233 BS4 64 1234 BS4 64 1235 BS4 64 1228 BS4 64 1229 BS4 64 1230 BS4 64 1231 BS4 64 1236 BS4 64 1237 BS4 64 1238 BS4 64 1239 BS4 64 1180 BS4 64 1181 BS4 64 1182 BS4 64 1183 BS4 64 1184 BS4 64 1185 BS4 64 1186 BS4 64 1187 BS4 64 1192 BS4 64 1193 BS4 64 1194 BS4 64 1195 U S Toll Free 800 272 2775 Fax 508 429 5732 Online Wire Pellet Body Style Port 1 16 barb 1 32 barb male Luer female Luer threaded 1 4 28 1 16 barb 1 32 barb male Luer MEW F10A MEW F12A MEW F15A MEW F20A MEW F10B MEW F12B MEW F15B MEW F20B MEW
57. 00TF 4 2 0 1 56 100mm 125 BS4 64 0770 ene A 0 69 150mm 350 BS4 64 0809 G200TF 6 2 0 Vale iS alin ze BS4 64 0771 G150 3 1 5 0 86 75mm 225 Premium Theta Glass BS4 64 0772 G150 4 1 5 0 86 100mm 225 BS4 64 0773 G150 6 11 5 0 86 150mm 225 Premium Theta Glass 100 pcs pkg BS4 64 0774 G200 3 2 0 1e aS mm 25 Catalog No Model OD mm Septum Length BS4 64 0775 G200 4 2 0 1 16 100mm 125 BS4 64 0810 TG150 4 1 5 1 0 0 2mm 100mm BS4 64 0776 G200 6 2 0 1 16 150mm 125 BS4 64 0811 TG200 4 2 0 1 4 0 2mm 100mm U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M45 Capillary Glass Specialized Tools For Bioresearch M46 Patch Clamp Glass Choosing patch clamp glass is generally based on noise perform ance and the ability to form and maintain a seal No one type of glass works best in all applications and some trial and error is usu ally required to find the type yielding optimum results in your experiment Patch glass is manufactured without an inner fila ment This glass ts offered in both the standard and premium mod els The premium glass has fire polished ends and is cleaned with deionized water before being packed in dust free containers Custom 8520 Patch Glass a Now Available in Premium Line The custom patch glass from Clark was introduced in 1997 as a sub stitute for the Corning 7052 glass a favorite amongst researchers per forming patch clampin
58. 04 BS4 64 0868 BS4 64 1079 BS4 64 1080 BS4 64 1081 BS4 64 0974 BS4 64 1082 BS4 64 1357 BS4 64 1358 BS4 64 1359 BS4 64 1360 BS4 64 1361 BS4 64 1362 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com p a 1 0 12 1 5 U 1 5 ile 2 0 1 7 2 0 QRW B10N QRW B12N QRW B15N QRW B17N QRW B20N QRW B10P QRW B12P QRW B15P QRW B17P QRW B20P QSH B15N QSH B17N QSH B20N QSH B15P QSH B17P QSH B20P __ SECS port ASojo1g 129 M39 Electrode Holders PE Series Holders E Applications ammm e y e Microperfusion perfusion at the pipette tip Tooo OO O o l p p pip p SAT em i _ Body Material Polycarbonate Fp ie Ae Body Style Straight Connectors 1 mm Pin 1 mm Pin Threaded and BNC Coupling Ag Wire Port 2 mm diameter Polycarbonate Perfusion Port 30 port accepts tubing up to 1 mm diameter Standard Glass Sizes 1 0 1 2 1 5 1 7 and 2 0 mm PESW TxxP Patch Clamp Compatibility Connector For Use With 1 mm Pin Warner Patch Clamp Models PC 501 amp PC 505 Axon Patch Clamps prior to 2 1 95 BNC List Heka and Dagan Patch Clamps For Microperfusion perfusing inside the micropipette Perfusion at the electrode tip is possible with the PE Series holders They include an additional port at 30 to the glass bore allowing for the insertion of micro bore tubing into the pipette This port includes a threaded cap and seal f
59. 17 The RC 27L chamber is designed for slice studies where solution above and below the slice is desired The large bath size and platform design also provide good access for immersion optics and electrodes The slice rests on a support 0 5 mm above the coverslip and is held in place by a slice anchor hold down Both support and hold down use fine lycra thread to secure the slice in place allowing maximum access to the preparation Thread spacing is 1 mm on the slice sup port Choice of Slice Hold Down Two hold down styles are available a stainless steel harp and a rectangular plastic frame While the weight of the metal harp holds the slice in place the plastic frame relies on a close apposition between itself and the side walls of the bath Hold downs are available with thread spacing of 1 1 5 and 2 mm and are sold separately See accessoreis for selection of slice hold down Material Polycarbonate Bath Dimensions Size L x W 38 x 18 mm Specimen Area 23 x 18 mm Volume 560 ul per mm of solution height Std Accessories Supplied 1 SS 3 Slice Support 2 PE 160 Tubing 10 3 24 x 50 mm 1 Coverslip pkg of 50 Platforms Used With 1 P 6D Plain Platform 2 PH 6D Heater Platform Catalog No Model Product BS4 64 0241 RC 27L RC 27L Chamber Accessories Spare or Replacements BS4 64 0273 SS 3 SliceSupport BS4 64 0256 SHD 27LH 10 Metal Slice Hold Down 1 mm BS4 64 0257 SHD 27LH 15 Metal Slice Hold Down 1 5 mm BS4
60. 2 6512 BS4 72 6492 BS4 72 6502 20 BS4 72 7584 BS4 72 7589 BS4 72 6488 BS4 72 6513 BS4 72 6493 BS4 72 6503 30 BS4 72 7585 BS4 72 7590 BS4 72 6489 BS4 72 6514 BS4 72 6494 BS4 72 6504 35 BS4 72 7586 BS4 72 7591 BS4 72 6490 BS4 72 6515 BS4 72 6495 BS4 72 6505 11 000 Specialty Products to Enhance Your Bioresearch Microinjection amp Microperfusion Screw Actuated Air Syringes These air assisted microinjec tion micro aspiration syringes provide the user with sensitive control and an extremely low dead air volume The capacity of the syringe is 10 ml When required the syringes can be used to generate high pressure or suction e For air assisted micro injection and micro aspiration e Uncomplicated and easy to use e Accurate e Sensitive control e High suction pressure The screw actuated syringe is a small compact unit incorporated into a heavy circular base Its con venient dimensions enable it to be ergonomically positioned next to the control lever of a micromanipulator to facilitate single handed operation of both instruments The syringe is connected by hard polyethylene tubing to a micro pipette which in turn may be fitted to a micromanipulator or positioning device Suction injection is obtained by turning the metal colored actuator screw control on the top of the syringe The syringe provides a solution to the problem of capillary action when working with small micropipettes A small balance
61. 3 mm 0 23 mm 0 23 mm 0 30 mm 0 30 mm 0 30 mm Septum 0 17 mm 0 17 mm 0 17 mm 0 22 mm 0 22 mm 0 22 mm 75 mm 100 mm 150 mm 75 mm 100 mm 150 mm Quantity 100 100 100 100 100 100 100 100 100 For Microforges see pages M53 and M54 For our full line of Pipette Pullers see pages M51 and M52 11 000 Specialty Products to Enhance Your Bioresearch Clark Capillary Glass Borosilicate Multi Barrel Borosilicate Multi Barrel Catalog No BS4 30 0003 BS4 30 0001 BS4 30 0002 BS4 30 0007 BS4 30 0008 BS4 30 0012 BS4 30 0013 BS4 30 0014 BS4 30 0015 079 1 5 mm 1 5mm 1 5mm 1 2mm 1 2 mm 1 2mm 1 2 mm 1 0 mm 1 0 mm 0 86 mm 0 86 mm 0 86 mm 0 69 mm 0 69 mm 0 69 mm 0 69 mm 0 58 mm 0 58 mm Borosilicate Glass Rod Borosilicate Glass Rod Catalog No BS4 30 0087 BS4 30 0085 BS4 30 0086 BS4 30 0088 1 0 mm 1 0 mm 1 0 mm 2 0 mm of Septum 75mm 100 mm 150 mm 100 mm 150 mm 100 mm 150 mm 100 mm 150 mm 75mm 100 mm 150 mm 100 mm Barrels Quantity N N OO O88 WO WD PTO WW PS 100 100 100 100 100 65 65 60 60 Quantity 500 500 500 125 Capillary Glass Aluminosilicate Capillaries In recent years there has been a developing interest in fabricating micropipettes from aluminosilicate glass Like silicon aluminum combines with oxygen to form tetrhbedral networks and the Al O bonds are very strong In comparison with borosilicate glass a
62. 30 0035 1 0mm 0 78mm 100mm 500 BS4 30 0016 1 0mm 058mm 100mm 500 BS4 30 0036 1 0mm 0 78mm 150mm 500 BS4 30 0017 1 0mm 058mm 150mm 500 BS4 30 0049 1 2mm 094mm 75mm 350 BS4 30 0043 1 2mm 069mm 75mm 350 BS4 30 0047 12mm 0 94mm_ 100mm 350 BS4 30 0042 1 2mm 0 9mm 100 mm 350 BS4 30 0048 1 2mm 0 94mm_ 150mm 350 BS4 30 0041 1 2mm 0 69mm_ 150mm 350 BS4 30 0064 1 5mm 1 147mm 50mm 225 BS4 30 0056 15mm 0 86mm 75mm 225 BS4 30 0065 iSmm diay mime 75mm 225 BS4 30 0054 15mm 0 86mm 110 mm 225 BS4 30 0062 1 5 mm 1 47mm 100 mm 225 BS4 30 0053 1 5mm 0 86mm 100mm 225 BS4 30 0063 15mm imm 150mm 225 BS4 30 0055 15mm 0 86mm 150mm 225 BS4 30 0073 20mm 1 16mm 75mm 125 BS4 30 0070 2 0mm 1 146mm_ 100mm 125 BS4 30 0071 2 0 mm 1 16mm 150 mm 125 BS4 30 0127 3 0mm 1 62mm 75mm 55 BS4 30 0080 3 0mm 1 62mm 100mm 55 BS4 30 0081 3 0mm 1 62mm_ 150mm 55 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M47 Capillary Glass m Specialized Tools For Bioresearch M48 Borosilicate with Filament Catalog No BS4 30 0034 BS4 30 0032 BS4 30 0033 BS4 30 0021 BS4 30 0019 BS4 30 0020 BS4 30 0046 BS4 30 0044 BS4 30 0045 BS4 30 0060 BS4 30 0057 BS4 30 0058 BS4 30 0076 BS4 30 0074 BS4 30 0075 BS4 30 0084 BS4 30 0082 BS4 30 0083 BS4 30 0040 BS4 30 0038 BS4 30 0039 BS4 30 0052 BS4 30 0050 BS4 30 0051 BS4 30 0068 BS4 30 0066 BS4 30 0067 BS4 30 012
63. 4 0378 111 KIT Silicone Grease Kit with Brushes and Pallets M90 11 000 Specialty Products to Enhance Your Bioresearch Chambers for 35mm Cell Culture Dishes Models RC 37WS and RC 37FS Perfusion Chamber Inserts with Field Stimulation 7 E E e e gt lt k Platinum Agar Bridge Well e Designed for glass bottom 35 mm dishes e Permits imaging and perfusion in the cell culture dish e Platinum field stimulation electrodes e deal for rapid screening assays Perfusion Outlet Port RC 37WS AAAA ae J Wires 7 i a al d rd r RC 37FS The latest member of the RC 37 family of perfusion chamber inserts is designed for applica tions requiring field stimulation and includes a pair of platinum electrodes Both versions feature a slotted bath to promote a laminar solution flow and to ensure an even electrical field These models include a perfusion inlet port and a 16 gauge height adjustable aspirator An agar bridge well has been added to accommodate a bath ground electrode A thin coating of silicone grease forms the seal between dish and chamber The RC 37WS fits in the Willco Wells dishes the RC 37FS fits the Falcon Corning 35 mm dishes Warner s DH 35 Culture Dish Heater may be used to heat the Corning and Wilco Wells 35 mm culture dishes while Falcon 35 mm dishes are compatible with Warner s DH 35i Culture Dish Incubator or the QE 1 Quick Change Heated Base Both chambers are
64. 4 64 1265 1 32 barb MP S10B BS4 64 1266 MP S12B BS4 64 1267 MP S15B BS4 64 1268 MP S20B BS4 64 1273 male Luer MP S10ML BS4 64 1274 MP S12ML BS4 64 1275 MP S15ML BS4 64 1276 MP S20ML BS4 64 1269 female Luer MP S10FL BS4 64 1270 MP S12FL BS4 64 1271 MP S15FL BS4 64 1272 MP S20FL BS4 64 1278 threaded l MP S10T BS4 64 1279 1 4 28 2 MP S12T BS4 64 1280 MP S15T BS4 64 1281 MP S20T M42 11 000 Specialty Products to Enhance Your Bioresearch Theta Glass Holders m SEEE Specifications Applications AEE E Body Material e Microinjection or microiontophoresis with Body Style theta glass Coupling Connector Port Standard Glass Sizes Handle Theta Glass Holders Catalog No Handle Electrode Holders Acrylic Straight Ag wire 2 2 mm jack THS FxxP 2 mm diameter polycarbonate 1 5 and 2 0 mm 6 3 mm diameter x 6 3 cm long threaded to attach to holder Glass OD mm Model Port BS4 64 1164 No No 1 5 THS F15 BS4 64 1172 ee C THS F15H BS4 64 1173 2 0 THS F20H BS4 64 1168 Yes No 1 5 THS F15P BS4 64 1169 2 0 THS F20P BS4 64 1176 Yes 1 5 THS F15PH BS4 64 1177 2 0 THS F20PH Electrode Manifold Holders MHH 25 MHH 38 Specifications The MHH 25 and MHH 38 Holders permit convenient mounting of manifolds and elec trode holders or other devices with 6 3 or 9 5 mm diameters The holder head can be pivoted 90 from the axial position and rotated 360 about the axis Friction holds the hea
65. 4 69 0130 Nanoject II Nanoliter Injector 115 V 60 Hz US Plug BS4 69 0131 Nanoject II Nanoliter Injector 230 V 50 Hz European Plug BS4 69 0132 Nanoject II Nanoliter Injector 230 V 50 Hz UK Plug Replacement Parts BS4 69 0133 Replacement Glass 88 9 x 1 14 x 0 053 mm 3 5 x 0 045 x 0 021 in L x OD x ID Vial of 100 BS4 69 0134 Replacement Glass 177 8 x 1 14 x 0 053 mm 7 x 0 045 x 0 021 in L x OD x ID Vial of 100 BS4 69 0135 Flared Glass 90 mm 3 5 in Vial of 100 The Nanoject II is the latest devel BS4 69 0136 Standard O Ring Soe UE opment in the Nanoject family of B54 69 0137 Special Flared O Ring capabiiiy microinjectors One of the unique I l BS4 69 0138 Replacement Wire Plunger e Positive displacement features of the Nanoject II is the BE S etn Tee ology use of a higher torque motor than i l paray 0 UREA MRAN a 2 3 to 69 0 nl range in previous models This new Acessories oryorlines motor is smoother in operation 85469 0140 Footswitch e Minimal vibration resulting in substantially reduced BS4 69 0141 Support Base e New improved high tip movement A new collect con BS4 69 0142 Universal Mounting Adapter see below photo torque motor figuration holds the micropipette more securely thereby reducing air infiltration and oil leakage The two speed fill inject mode will allow the user to fill and inject at half speed facilitating the use of smaller micropipettes The handling of more viscous samples will also be improved
66. 5 microseconds to 1 5 milliseconds Measurements can be carried out either at constant frequency or constant phase shift This technique differs from other methods of O detection in that this method is total ly noninvasive with the fiber optic bundle outside of the tissue in which the O is being measured The PMOD 2000 has a very high accuracy throughout the physiological range and provides real time measurements with high temporal resolution 1 sec measure ment The phosphors that are used with this system have absolute calibration and follow Stern Volmer behavior Once a phosphor is calibrated for a given temperature and solvent conditions these val ues are stable eliminating the possibility of measurement drift and the requirement for re calibration at the time of the measurement The system has two channels sources of excitation light The stan dard system comes with one wavelength set to 524 nm suitable for the porphyrin based phosphors and 635 nm suitable for the tetrabenzo porphyrin based phosphors Other combinations of excitation wave lengths are available Please call our technical sales department for fur ther information Phosphorescence emission is in the range of 660 to 760 nm porphy rins or 760 to 900 nm tetrabenzoporphyrins Since bright ambi ent light can saturate the detector it is recommended that the work area be lighted with DC powered monochromatic light sources The Cool Colors LED lamps are spe
67. 6 1997 9 Vinogradov S A Lo L W and Wilson DE 1999 Dendritic polyglutamic porphyrins probing porphyrin protection by oxygen dependent quenching of phosphorescence Chem Europ J 5 9 1388 1347 Selected Application Reports selected from more than 60 D Yonetani M Huang Ch Ch Lajevardi N Pastuszko A Delivoria Papadopoulos M and Wilson DE 1994 Effect of hemorrhagic hypotension on extracellular level of dopamine cortical oxygen pressure and blood flow in brain of newborn piglets Neuroscience Letters 180 247 252 2 Rumsey WXL Pawlowski M Lejavardi N and Wilson DE 1994 Oxygen pressure distribution in the heart in vivo and evaluation of the ischemic border zone Am J Physiol 266 H1676 1680 3 Huang Ch Ch Yonetani M Lajevardi N Delivoria Papadopoulos M Pastuszko A and Wilson D E 1995 Comparison of post asphyxial resuscitation with 100 and 21 oxygen on striatal dopamine metabolism in newborn piglets J Neurochemistry 64 292 298 4 Song D Olano M Wilson D E Pastuszko A Tammela O Nbo K and Shorr R G L 1995 Comparison of the efficacy of blood and polyethylene glycol bemoglobin in recovery of newborn piglets from hemorrhagic hypotension effect on blood pressure cortical oxygen and extracellular dopamine in the brain Transfusion 35 552 558 5 Poole D C Wagner PD and Wilson D E 1995 Diaphragm microvascular plasma PO measured in vivo J Appl
68. 6 pA nM typical 4 pA nM n a BS4 69 0010 2 amiNO 100 100 um 25 to 50 pA nM typical 35 pA nM No Equivalent BS4 69 0011 2 amiNO 600 600 um 150 to 250 pA nM typical 200 pA nM No Equivalent BS4 69 0012 1 amiNO 2000 2mm Typical 1 pA nM Typical 1 pA nM BS4 69 0006 2 amiNO IV 600 ym 150 to 250 pA nM typical 200 pA nM No Equivalent BS4 69 0007 1 amiNO FLAT See diagram 250 to 700 pA nM typical 400 pA nM No Equivalent inNO T Nitric Oxide Measurement Systems Premium System 1 Premium System 2 Premium System 3 Premium System 4 Sensors and Accesories 115 VAC 230 VAC 115 VAC 230 VAC 115 VAC 230 VAC 115 VAC 230 VAC Catalog No System Components 60 Hz 50 Hz 60 Hz 50 Hz 60 Hz 50 Hz 60 Hz 50 Hz BS4 69 0012 amiNO 2000 Nitric Oxide Sensor 2mm Tip BS4 69 0010 amiNO 100 Nitric Oxide Sensor 100 um Tip pkg of 2 BS4 69 0011 amiNO 600 Nitric Oxide Sensor 600 um Tip pkg of 2 BS4 69 0006 amiNO IV Nitric Oxide Sensor In Vivo 600 Micrometer Tip pkg of 2 BS4 69 0007 amiNo FLAT Nitric Oxide Sensor Flat 25 x 25 mm pkg of 1 BS4 69 0013 Electrolyte for BS4 69 0012 BS4 69 0014 Replacement Membranes for BS4 69 0012 pkg of 4 BS4 69 0037 Nitrite Standard 50 ml BS4 69 0038 Calibration Vials pkg of 5 BS4 69 0016 SensoReady Electrode Polarizer BS4 72 0460 Spin Bar Magnetic Stir Bar 5 x 2mm pkg of 5 BS4 72 1920 Magnetic Stirrer 115 VAC BS4 72 1921 Magnetic Stirrer 230 VAC BS4 72 1922 Electrode Holder a
69. 60 Hz Cool Color Lamp Red 638 nm maximal intensity 230 VAC 50 Hz Cool Color Lamp Green 524 nm maximal intensity 115 VAC 60 Hz Cool Color Lamp Green 524 nm maximal intensity 230 VAC 50 Hz Phosphor Pd meso tetra 4 carboxyphenyl porphyrin 1 gm Vial Oxyphor RO Phosphor Pd meso tetra 4 carboxyphenyl porphyrin dendrimer 1 gm Vial Oxyphor R2 Phosphor Pd tetra 4 carboxypheny tetrabenzoporphyrin dendrimer Oxyphor G2 20 mg Phosphor Pd tetra 4 carboxypheny tetrabenzoporphyrin dendrimer Oxyphor G2 50 mg Phosphor Pd tetra 4 carboxypheny tetrabenzoporphyrin dendrimer Oxyphor G2 100 mg 11 000 Specialty Products to Enhance Your Bioresearch FO ton BS4 69 0450 FO ton Fiber Optic Oxygen Sensing System HARVARD f Fofon T RARD Foren The FO ton oxygen sensing system detects oxygen pO in both solutions or in air through the use of a ruthenium fluorescence and Fiber Optic Technology The FO ton sys tem offers high sensitivity reversability and stability in the detection of oxygen in numer ous applications Its low power require ments and small package size makes it ideal for remote monitoring field applications and other applications where size and or portability are required The thin film ruthenium technology used in the probe tips for detecting oxygen does not consume oxygen allowing the sensor tip to be in continuous contact with the sample FO ton Oxygen Sensors work
70. 64 0258 SHD 27LH 2 Metal Slice Hold Down 2 mm BS4 64 0259 SHD 27LP 10 Plastic Slice Hold Down 1 mm BS4 64 0260 SHD 27LP 15 Plastic Slice Hold Down 1 5 mm BS4 64 0261 SHD 27LP 2 Plastic Slice Hold Down 2 mm Note RC 27L Chambers require Platform P 6D of PH 6D see page M112 and Microscope Stage Adapter see pages M114 and M115 11 000 Specialty Products to Enhance Your Bioresearch Special Chambers Systems Excised Patch Chamber RC 28 TOP VIEW Dam Reservoir Outlet END VIEW Dam Reservoir C Small Bath a Large Bath Perfusion Spreader Perfusion Inlets Dam Reservoir Inlet Suction Wells SIDE VIEW 4 me i i o 50 mm _ This chamber is designed for electrophysiological recordings of excised patches The dual bath construc tion permits exchange of the solution surrounding the membrane patch without contamination of the entire population The chamber consists of two independent bath compartments separated by a dam reservoir which functions as a gate and barrier to prevent mixture of the solutions The composition and exchange rate of each bath can be controlled independently After patching onto a cell in the larger bath compart ment the dam reservoir is flooded with solution estab lishing a solution bridge between the two compart ments This enables secure transfer of the electrode attached cell to the smaller compartment The dam reservoir is then emptied by vacuum suction thus separating the
71. 7 to M49 for mm 4 in L 30 ga 305 ym 0 012 in OD the industry standard capil BS4 69 0448 AK15 Glass head pipettes 3 mm OD pkg of 100 lary glass BS4 69 0394 MF 11 Accessory Kit without weights BS4 69 0395 MF 12 Filament Holder Assembly with Center Rod 6 4 x 51 mm 0 25 x 2 in OD x L M54 11 000 Specialty Products to Enhance Your Bioresearch Electroporation Products MOS 25 amp MOS 96 Advanced Electroporation Systems HARVARD APPARATUS Molecular Delivery Systems Features and Benefits e Methods optimization e Multi well plates can be electroporated in less than 10 minutes e Integrated electrodes e Fast and precise molecular delivery e Rapid and easy experimental optimization e High throughput approach to experimentation e Multiple experiments in the sample plate e Rapid analysis in a matter of minutes research 96 experimental parameters e Disposable For ease of use and no carryover Applications Include e Transfection of Mammalian Cells e Transformation of Bacteria and Yeast e cDNA Library Screening e Drug Screening The Future of Electroporation Is Here BTX the leader in electroporation products and specialty electrodes has developed a new and innovative product line for experiment optimization and large volume electroporation The Advanced Electroporation System is a breakthrough in Molecular Delivery The days of researcher s struggling with tedious multi step procedures that typic
72. 8 BS4 30 0077 BS4 30 0078 0 1 0 mm 1 0 mm 1 0 mm 1 0 mm 1 0 mm 1 0 mm 1 2 mm 1 2 mm 1 2mm 1 5mm 1 5mm 1 5mm 2 0 mm 2 0 mm 2 0 mm 3 0 mm 3 0 mm 3 0 mm Borosilicate Thin Wall with Filament 1 0 mm 1 0 mm 1 0 mm 1 2mm 1 2 mm 1 2 mm 1 5mm 1 5mm 1 5mm 2 0 mm 2 0 mm 2 0 mm Clark Capillary Glass Borosilicate with Filament Borosilicate Standard Wall with Filament 0 50 mm 0 50 mm 0 50 mm 0 58 mm 0 58 mm 0 58 mm 0 69 mm 0 69 mm 0 69 mm 0 86 mm 0 86 mm 0 86 mm 1 16 mm 1 16 mm 1 16 mm 1 62 mm 1 62 mm 1 62 mm 0 78 mm 0 78 mm 0 78 mm 0 94 mm 0 94 mm 0 94 mm 1 17 mm 1 17 mm 1 17 mm 1 56 mm 1 56 mm 1 56 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm 75mm 100 mm 150 mm MOTTE TULA 500 500 500 500 500 500 350 350 350 225 225 225 125 125 125 55 55 55 500 500 500 350 350 350 225 225 225 125 125 125 Borosilicate Double Barrel Special Catalog No BS4 30 0004 BS4 30 0005 BS4 30 0006 OD 15 15 15 Borosilicate Theta Glass Catalog No BS4 30 0116 BS4 30 0114 BS4 30 0115 BS4 30 0119 BS4 30 0117 BS4 30 0118 OD 1 5 mm 1 5 mm 1 5 mm 2 0 mm 2 0 mm 2 0 mm Borosilicate Double Barrel Special 0 86 mm 75 mm 0 86 mm 100 mm 0 86 mm 150 mm Borosilicate Theta Glass 0 2
73. BS4 61 0250 Teflon Stirring Bars 2 x 7 mm pkg of 3 BS4 61 0248 Dual Channel Precision Precision Stirring Controller Stirring Controllers Our BS4 61 0247 and BS4 61 0248 Stirring Controllers are used to control the magnetic stirring assembly in the batch cell chamber Each controller has an on off switch a power on LED and a dial to precisely regulate the motor speed from 200 to 5000 RPM The controllers use a unique pseudo tachometer feedback cir cuit which reduces electromagnetic interference The BS4 61 0248 dual channel is a lower cost per channel option than the BS4 61 0247 It is also the most frequently ordered model since many oxygen experiments require two channels Product Single Channel Precision Stirring Controller Catalog No BS4 61 0247 BS4 61 0248 Dual Channel Precision Stirring Controller 11 000 Specialty Products to Enhance Your Bioresearch Respirometry HSE HA Oxystat System Type 1000 Stimulation Electrode Connection Stirrer l Motor Application The oxygen consumption VO of small cell assemblies e g papillary muscle or of isolated cells has usu ally in the past been calculated from the reduction in the oxygen partial pressure pO in a closed system By contrast the HSE HA Oxystat Operates as an open system with feedback The pO as the con trolled variable is maintained con stant by the addition of solution with known pO VO can be calculate
74. CS18R15 BS4 64 0715 CS25R15 Volume 308 pl Input Output Tubing PE 90 a Product Quick Change Chamber High Profile Quick Change Chamber Closed Bath Quick Exchange Heated Base for RC 40 Series Coverslips 18 mm Dia 1 Box 100 Coverslips 25 mm Dia 1 Box 100 Coverslips 18 mm Dia 1 5 Box 100 Coverslips 25 mm Dia 1 5 Box 100 11 000 Specialty Products to Enhance Your Bioresearch Micro Incubation Series 40 Slotted Bath Low Profile Chambers for 25 mm Coverslips RC 46SNLP RC 46SLP Low profile design permits low entry angle of electrodes Slot shaped bath for applications requiring rapid perfusion Platinum electrodes for applications requiring field stimulation When used with QE 1 Quick Exchange Platform both temperature control and perfusion are possible RC 47FSLP The RC 46SLP RC 46SNLP and RC 47FSLP Chambers use a 25 mm round coverslip in a slotted bath design to provide rapid perfusion exchange O rings seal the coverslip to the polycar bonate chamber and hold the chamber securely in the alu minum base The low volume of these slotted bath chambers and the natural laminar flow allows rapid exchange of solution facilitating drug application studies The RC 47FSLP includes a pair of platinum wires placed onto the parallel sides of the oval shaped bath The connecting wires are ter minated in 1 mm pins This model includes a perfusion inlet port and a
75. Cell Biology NEN Nitric Oxide Measurement Systems Cc Oxygen Measurement Systems CD Respirometry Systems e pH and Reference Electrodes 6 Channel Oxygen System Model 928 see page M18 Microelectrode Holders e Capillary Glass e Micropipette Pullers RN NEN Electroporation Products Electroporation Electrodes and Accessories MOS 25 and MOS 96 Advanced Electroporation Systems see page M55 Microinjection and Microperfusion Systems Micro Incubation Products Chambers for 35mm Cell Culture Dishes Cc Special Chambers Systems Cc Chamber Platforms and Stage Adapters Cw Temperature Controllers Temperature Controller Peripherals Cc Perfusion Controllers NEN Microscopes and Illuminators WENN Tissue Sampling Systems NEN Diffusion Ussing Chambers EWN Voltage Current Clamps Cc Micromanipulation Products SC 20 Dual In Line Solution Heater Cooler see page M130 e Cell Biology Books Dovetail Slide Micromanipulators see page M191 U S Toll Free 800 272 2775 Fax 508 429 5732 Online see pages M2 M5 see pages M6 M14 a see pages M15 M22 see pages M23 M31 see pages M32 M44 see pages M45 M49 see pages M50 M54 see pages M55 M61 see pages M62 M69 see pages M70 M79 see pages M80 M86 see pages M87 M93 see pages M94 M111 see pages M112 M125 see pages M124 M129 see pages M130 M134 see page
76. Chamber Slides and Chambered Coverglass from the Nunc Lab Tek series and the Falcon Cultureslide series from Beckton Dickinson The CSMI in combination with a matching low noise TC 202A temperature controller facilitates long term maintenance of tissue or cell cul tures on a microscope stage This allows optical monitoring of dyes micro injection electrical record ing and micro manipulation for many hours or even days The CSMI not only facilitates the precise regulation and manipula tion of bath temperature but also supports multi channel perfusion up to 4 channels and gas atmosphere maintenance such as CO super fusion for pH control The CSMI is based on a successful design of the Medical Systems PDMI 2 micro incubator for 35 mm Petri dishes The CSMI utilizes a Peltier thermo electric device to regulate temperature over a wide range both above and below ambient levels Lower temper atures approximately 10 C below ambient may be obtained by using an optional cooling tube The CSMI fits on the stage of inverted microscopes from major micro scope manufacturers including Zeiss Nikon Olympus and Leica Harvard Apparatus offers custom made rectangular adapters to these microscopes Harvard Apparatus Inc offers a comprehensive line of versatile Micro Incubators and cell tissue holding chambers for use on the stages of microscopes These Micro Incubators allow researchers to control temperature and pH thus s
77. E BS4 45 0160 508 KIT Includes Straight 5 mm Electrode Gold In Vivo Applications Model 508 and Model 510 Tip BS4 45 0113 Holder BS4 45 omm The Model 508 Genetrode with a sharp 5 mm electrode tip is a general 0203 and Cables BS4 45 0201 and in vivo electrode The Model 510 is the same electrode but with a 10 mm AE 0 oO tip Similar electrodes have been used for a variety of applications 854 45 0161 510 KIT Includes Straight 10 mm Electrode Yo Salford used an electrode of this style with a 600 Series to deliver Gold Tip BS4 45 0114 Holder BS4 e ook l re 45 0203 and Cables BS4 45 0201 bleomycin into rat gliomas doubling animal survival time Yamaguchi and BS4 45 0210 N used a similar electrode with an 800 Series to deliver bleomycin into seu aie ear roed Cann subcutaneously grafted mouse bladder carcinoma resulting in complete Electrode Gold Ti a BS 4 45 0115 regressions if the tumorous tissue Nishi used this type of electrode with Holder BS4 45 0203 and Gables a BTX Generator to deliver a plasmid DNA construct into rat gliomas BS4 45 0201 and BS4 45 0210 resulting in expression of beta galactosidase in the area between the BS4 45 0163 514KIT Includes Bent L shaped 3 mm electrodes Muramatsu used similar electrodes with a BTX Generator in Electrode Gold Tip BS4 45 0116 his studies of LIVGET techniques in mouse testis concluding that elec Holder BS4 45 0203 and Cables troporation is superior to micr
78. G200F 3 2 0 116 75mm 125 mal stress It is also easy to work Dielectric Constant 4 6 BS4 64 0796 G200F 4 2 0 1 16 100mm 125 O with and suitable for a wide BS4 64 0797 G200F 6 2 0 1 16 150mm 125 range of applications Corning 7740 glass is offered in a choice sa oo of diameters in standard or thin walled format and either with Premium Thin Wall Borosilicate Q m o or without inner filament they are additionally available in with Filament Theta style Single barrel glass is available in 3 lengths 75 100 Premium Thin Wall Borosilicate with Filament Oc and150 mm Theta glass is offered in 100 mm lengths only Catalog No Model OD mm ID mm Qty Pkg BS4 64 0798 G100TF 3 1 0 0 78 75mm 500 Premium Standard BS4 64 0799 G100TF 4 1 0 0 78 100mm 500 Wall Borosilicate BS4 64 0800 GIO00TF 6 1 0 0 78 150mm 500 BS4 64 0801 G120TF 3 1 2 0 94 75mm _ 350 Premium Standard Wall Borosilicate BSA 64 0802 G120TF 4 1 2 0 94 400mm 350 CatalogNo Model OD mm ID mm Length Qty Pke B54 64 0803 G120TF 6 1 2 094 150mm 350 BS4 64 0765 G100 3 1 0 058 75mm 500 BS4 64 0804 G150TF 3 1 5 1 17 75mm 225 BS4 64 0766 G100 4 1 0 0 58 100mm 500 BS4 64 0805 G150TF 4 1 5 1 17 100mm 225 BS4 64 0767 G100 6 1 0 0 58 150 mm 500 BS4 64 0806 G150TF 6 1 5 1 17 150mm 225 BS4 64 0768 Gi20 3 1 2 0 69 75mm_ 350 BS4 64 0807 G200TF 3 2 0 1 56 175 mm 1125 BS4 64 0769 G120 4 11 2 0 69 100mm 350 BS4 64 0808 G2
79. IMT 2 1X50 1X70 BX50WI BS4 64 0326 SA 30LZ w Stage Adapters for Zeiss Axiovert 211 x 230 Specimen Stage BS4 64 0327 SA 30KZ w Stage Adapters for Zeiss Axiovert 85 x 130 Mechanical K Stage BS4 64 0326 SA 30LZ Stage Adapters for Leica DMIRB E with Plane Stage BS4 64 0328 SA 30L3P Stage Adapters for Leica MIRB E with 3 Plate Mechanical Stage BS4 64 0329 SA 30PLI For Prior amp Ludi Motorized Stage BS4 64 0330 GS 30L 10 Replacement Gaskets for Large Bath 250 um Thickness pkg of 10 BS4 64 0331 GS 30L 15 Replacement Gaskets for Large Bath 375 um Thickness pkg of 10 BS4 64 0332 GS 30S 10 Replacement Gaskets for Slotted Bath 250 um Thickness pkg of 10 BS4 64 0333 GS 30S 15 Replacement Gaskets for Slotted Bath 375 um Thickness pkg of 10 BS4 64 0334 GS 30B 10 Replacement Gaskets for Blank 250 um Thickness pkg of 10 BS4 64 0335 GS 30B 15 Replacement Gaskets for Blank 375 um Thickness pkg of 10 BS4 64 0341 GS 30B 4 Replacement Gaskets for Blanks 150 um Thickness pkg of 10 BS4 64 0342 GS 30L 4 Replacement Gaskets for Large Bath 150 um Thickness pkg of 10 BS4 64 0343 GS 30S 4 Replacement Gaskets for Slotted Bath 150 um Thickness pkg of 10 Note see pages M114 and M115 for Stage Adapter drawings Exterior dimensions for corresponding models SA 20 vs SA 30 are identical 11 000 Specialty Products to Enhance Your Bioresearch Special Chambers Systems CD Model RC 50 Imaging Chamber for Transepith
80. Online www harvardapparatus com M65 Electroporation Electrodes Specialized Tools For Bioresearch M66 CD Caliper Electrodes Specifications Sheet The BTX Caliper Flectrodes are in vivo style re usable electrodes that are used for a variety of applications Caliper Electrodes come in two models with each model consisting of a caliper and a pair of end plates Model 384 are used for smaller ani mals with a smaller surface area while model 384L is used to target slightly larger surface areas The end plates of the Caliper Electrode may be adjusted by using the black roller mounted on the caliper Caliper Electrodes may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide Properly maintained Caliper Electrodes have an unlimited life and are compatible with most BTX Electroporation Systems Applications Caliper Electrodes may be used for many electroporation applications including drug and gene delivery The electrodes span the target area following injection of the molecule of interest and electroporation pulses are delivered using a BTX Electroporation System such as the ECM 630 or ECM 830 The electric field introduced by the Caliper Electrodes cause transient pores to form in the cells of the tissue allowing uptake of the molecules into the cells Zhang et al used the Caliper Electrodes to deliver molecules to skin for gene therapy studies on skin aging Mir et al used the Caliper Electro
81. PLI IHA and PLI 90 100 Gas Input Brass Screw Fitting Type Old Gas Input Hose Tygon Tubing 9 ft L x 0 250 in 6 35 mm OD x 0 125 in 3 18 mm ID x 0 062 in 1 57 mm Wall Thickness Connects to PLI IHA and PLI 90 100 Gas Input Quick Connect Disconnect Type New Output Hose Tygon Tubing 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders Brass Screw Fitting Type Old Output Hose Tygon Tubing 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 90 100 Gas Output Quick Connect Disconnect Type New Holding Hose with Cell Release Bulb 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 100 Hold Output Brass Screw Fitting Type Old Holding Hose with Cell Release Bulb 9 ft L x 0 071 in 1 80 mm OD x 0 039 in 1 mm ID x 0 016 in 0 41 mm Wall Thickness Connects to MSC Pipette Holders and PLI 100 Hold Output Quick Connect Disconnect Type New Harvard Apparatus now OWNS oeme Clark Electromedical See pages M45 to M49 for industry standard capillary glass Catalog No Model Product Pipette Holder Adapter Parts BS4 65 0013 BS4 65 0014 BS4 65 0017 BS4 65 0015 BS4 65 0018 BS4 65 0019 BS4 65 0020 BS4 65 0021 BS4 65 0022 Optional Easy Insert Acrylic
82. Pipette Holder J _ memg _______ 8 mm 0 312 in Se Capillary Glass OD Catalog No BS4 69 0867 BS4 69 0868 BS4 65 0023 BS4 69 0869 PLI PH1 Stainless Steel Pipette Holder 130 mm L for 1 to 1 5 mm OD Glass Pipettes PLI PH1A Stainless Steel Pipette Holder 80 mm L for 1 to 1 5 mm OD Glass Pipettes Silicone Rubber Gasket Replacement for use with PLI PH1 and PLI PH1A 10 2 cm 12 in Silicone Rubber Gasket Replacement for use with 1 5 mm Glass Pipettes PLI SRG PLI SRG1 5 PLI SSB Stainless Steel Bushing Replacement for use with PLI PH1 and PLI PH1A Pipette Holders PLI PC Stainless Steel Pipette Cap Replacement for use with PLI PH1 and PLI PH1A Pipette Holders Stainless Steel Hose Nut Replacement for use with PLI PH1 and PLI PH1A Pipette Holders PLI HN PLI PH KIT Pipette Holder Hardware Replacement Kit for use with PLI PH1 and PLI PH1A Pipette Holders Includes 1 each PLI SSB PLI PC PLI HN and PLI SRG Stainless Steel Pipette Holder PLI PH series Adapter for Eppendorf ECET FEMTOTIP PLI PHA Barrel Diameter G T Tubing Diameter 2 mm 0 08 in ASoj01g 129 Product Acrylic Pipette Holder for 1 0 mm Pipettes Model AO16 1 0 AO16 1 2 Acrylic Pipette Holder for 1 2 mm Pipettes PLI PPH Acrylic Pipette Holder for 1 5 mm Pipettes AO16 1 2 Acrylic Pipette Holder for 2 mm Pipettes Harvard Apparatus now owns the NaviCyte line of Ussing and D
83. Q and PE Series holders with mae threaded T connector Glass Seal Compression Caps BS4 64 1293 2 QC 10 Compression cap for 1 0 mm glass holders BS4 64 1294 2 QC 12 Compression cap for 1 2 mm glass holders BS4 64 1295 2 QC 15 Compression cap for 1 5 mm glass holders QC xx BS4 64 1375 2 QC 17 Compression cap for 1 7 mm glass holders BS4 64 1296 2 QC 20 Compression cap for 2 0 mm glass holders Connector Pins and Jacks BS4 64 1283 3 HC 10M 1 mm pin for Q and PE holders with A or T connectors BS4 64 1284 3 HC 13M 1 3 mm pin for Q and PE holders with B BNC connector BS4 64 1285 3 HC 20M 2 mm threaded pin for E ME and theta holders BS4 64 1286 3 HC 21F 2 mm jack assembly for all series holders BS4 64 1287 3 HC 22M 2 mm pin for Q and PE holders with M connector Ea Specialized Tools For Bioresearch M44 11 000 Specialty Products to Enhance Your Bioresearch Premium Capillary Glass Warner capillary glass is known worldwide for its consistent high quality The glass is precision drawn to insure reliability and con sistency from batch to batch The full line of glass capillaries listed here is stocked for fast shipment Standard Wall Thin Wall Tubing is available in two wall thickness standard wall and thin wall Additionally a variety of diameters is offered to cover most needs of micropipette and microelectrode research Capillaries with Filament Premium Thin Wall Borosilicate Capillary Glass Premiu
84. R Coverslips 25 mm Dia 1 Box of 100 M102 11 000 Specialty Products to Enhance Your Bioresearch Special Chambers Systems Series 20 Open Bath Recording Imaging Chambers RC 22 RC 22C TOP VIEW Suction Reservoir Perfusion Inlet Port Agar Bridge Well SIDE VIEW Suction Tube Slot mall m Volume 113 wl Volume 138 jl BATH DIMENSIONS RC 22 RC 22C ieee a o Volumes are for 1 mm solution Recording Chambers for Tissue Slice RC 22 113 pl amp RC 22C 138 pl Small bath chambers for tissue and brain slice studies Slices are held down with stainless steel anchors with lycra threads at 1 5 mm spacing Anchor weight is 300 milligrams One anchor is supplied with each chamber Bottom coverslip size is 22 x 40 mm Order with P 1 or PH 1 platform on page M112 Catalog No Model Product BS4 64 0228 RC 22 Recording Chamber for Tissue Slice with SHD 22L 2 Anchor BS4 64 0229 RC 22C Recording Chamber for Tissue Slice with SHD 22CL 2 Anchor Additional Anchors BS4 64 0246 BS4 64 0247 RC 22L 2 RC 22CL 2 Slice Anchor for RC 22 1 5 mm Slice Anchor for RC 22C 1 5 mm Ss RC 24 RC 24E TOP VIEW Suction Reservoir Suction Agar Bridge Well Tube Slot Perfusion Inlet Port Bath SIDE VIEW a a Jy amp cc r 138mm BATH DIMENSIONS mm 15mm Volume 40 ul Volume 50 ul Volumes are for 1 mm solution Fast Exchange Recording Chamber RC 24 40 pl amp RC 24E 50
85. SP M15P ESP M20P ESP F10N ESP F12N ESP F15N ESP F20N ESP F10P ESP F12P ESP F15P ESP F20P ESW M10N ESW M12N ESW M15N ESW M20N ESW M10P ESW M12P ESW M15P ESW M20P Wire Pellet Connector i m m 11 000 Specialty Products to Enhance Your Bioresearch E Series Holders E SERIES Straight Body Holders Continued Catalog No BS4 64 0999 BS4 64 1000 BS4 64 1001 BS4 64 1002 BS4 64 1003 BS4 64 1004 BS4 64 1005 BS4 64 1006 BS4 64 1007 BS4 64 1008 BS4 64 1009 BS4 64 1010 Wire Pellet Connector Wire 2 mm Jack 1 0 1 2 15 Vented models are standard with the Warner OC 725 Oocte Clamp E Series 45 Body Holders Catalog No BS4 64 0908 BS4 64 0909 BS4 64 0910 BS4 64 0911 BS4 64 0912 BS4 64 0913 BS4 64 0914 BS4 64 0915 BS4 64 0900 BS4 64 0901 BS4 64 0902 BS4 64 0903 BS4 64 0904 BS4 64 0905 BS4 64 0906 BS4 64 0907 BS4 64 0924 BS4 64 0925 BS4 64 0926 BS4 64 0927 BS4 64 0928 BS4 64 0929 BS4 64 0930 BS4 64 0931 BS4 64 0916 BS4 64 0917 BS4 64 0918 BS4 64 0919 Wire Pellet Connector E U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Electrode Holders Glass OD mm Model ESW F10N ESW F12N ESW F15N ESW F20N ESW F10P ESW F12P ESW F15P ESW F20P ESW F10V ESW F12V ESW F15V ESW F20V Glass OD mm Model E45P M10N E45P M12N E45P M15N E45P M20N E45P M10P E45P M12P E45P M15P E45P
86. Signal Output Ports Power Consumption Four PM 4 or eight PM 8 injection perfusion outputs one synchronized drain out and one cell hold channel 30 to 100 p s i Same as input pressure 0 to 3 p s i regulated 0 05 to 60 p s i regulated 0 05 to 10 p s i regulated 0 to 24 in Hg regulated 0 to 30 in of water regulated 0 to 30 in of water regulated 0 02 p s i 0 05 p s i for pressure 0 2 in for vacuum lt 2 in H O 10 msec to 327 67 sec 10 msec resolution for whole range 1 to 255 16 sequences 32 steps for each sequence DB9 connector for remote key pad foot switch jack and BNC for negative TTL trigger jack connector for injection foot switch Eight BNCs for injection signal on output port 1 to 4 100 120 or 220 VAC 35 W Cat No Qty Model Product BS4 69 0152 1 PM4 4 Channel Pressure Injection System BS4 69 0153 1 PM8 8 Channel Pressure Injection System BS4 69 0155 OP1 Output Tubing with Connector and 4 5 ml Vial BS4 69 0158 PM KP Remote Key Pad BS4 69 0160 RK 2 Rack Mounting Kit with Handles BS4 69 0152 Only BS4 69 0165 HOLD1 Holder for Single Barrel Pipette for BS4 69 0152 and BS4 69 0153 BS4 69 0166 HOLD4 Holder for 4 Barrel Pipette for BS4 69 0156 Output Tubing with Connector for use with BS4 69 0152 see above BS4 69 0153 see above BS4 69 0150 see page M52 BS4 69 0151 see page M52 BS4 69 0155 Output Tubing with Connector and 4 5 ml Vial for use with BS4 69 0152 see a
87. TUBE PDMI 2 Copper Cooling Tube Coil Product PDMI 2 PSMI Alignment Ring for Nikon Diaphot Old or 300 200 TMD Stage 4 242 x 071 in D x Thickness PDMI 2 PSMI Alignment Ring for Olympus 1X50 70 or IMT 2 Fixed Stage 4 320 x 071 in D x Thickness PDMI 2 PSMI Alignment Ring for Zeiss Axiovert Gliding or Rotary Stages 4 046 x 071 in D x Thickness PDMI 2 PSMI Fixing Platform for Leica DAS Mikroskop DMIL and DMIRB E with Attachable Mechanical Stage PDMI 2 PSMI Fixing Platform for Zeiss Axiovert with Attachable Mechanical Stage M Type Stage LU CB 1 Fixing Platform for Leica DAS Mikroskop DMIL and DMIRB E with Attachable Mechanical Stage LU CB 1 Fixing Platform for Zeiss Axiovert with Attachable Mechanical Stage M Type Stage Catalog No Model Alignment Rings and Fixing Platforms BS4 65 0059 PDMI ARN BS4 65 0060 PDMI ARO BS4 65 0061 PDMI ARZ BS4 65 0062 PDMI FPL BS4 65 0063 PDMI FPZ BS4 65 0064 LU FPL BS4 65 0065 LU FPZ BS4 65 0093 PDMI ARL U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Alignment Ring for Leica Microscope 88 x 1 8 mm OD x Thickness Micro Incubator Replacement Parts BS4 65 0066 BS4 65 0067 BS4 65 0068 BS4 65 0069 BS4 65 0070 BS4 65 0071 BS4 65 0072 D ORING 1 D ORING 2 CS GLASS 1 CS GLASS 2 MI T LU CPC P TC CA Shaped to Conform to Fin Channel with Open Ends Cover Slip Dish O Rings Replacement fo
88. VIEW 2 5 mm PIN BATH DIMENSIONS RC 21BRFS l pu 6 3 mm Volume 263 Volumes are for 2 5 mm solution height Imaging Chamber with Field Stimulation RC 21BRFS 263 pl 2 5 mm RC 21BRFS is an imaging chamber with field stimulation electrodes A pair of platinum wires is epoxied into the parallel sides of the oval shaped bath The connecting wires are terminated in 1 mm pins The chamber uses a pair of 25 mm round coverslips Bath volume is 260 pl for 2 5 mm of solution The additional port at the chamber per fusion input may be used for removing bubbles or injecting a sub stance At the bath output a length of tubing is used as a back pressure control to insure a smooth perfusion flow Order with P 2 or PH 2 plat form see page M112 Model Product RC 21BRFS Imaging Chamber with Field Stimulation Catalog No BS4 64 0226 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M101 Special Chambers Systems Series 20 Open Bath Recording Imaging Chambers Open bath models are designed for electrophysiological recording and or optical imaging and are used in applications including patch clamp oocyte clamp and physiological measurements on cell cultures and tissue preparations The low profile design permits good electrode access to the bath Agar bridge wells for reference electrodes are provided TOP VIEW Suction Tube A Qy ACA a rc a a I e pa
89. a or elongated b orifice is formed within the capillary Piercing a Glass Wall a a projecting tips b b flat tip c passage of a metal wire through an aperture as a then sealing it by fusion A flat plane may be per forated in the same manner Model E Spatula Piece of triangular glass whose corners have been fire polished and then affixed to a glass rod J Type of Scalpel A small globule of glass is affixed to filament Then a platinum wire embedded in a glass capillary enters the surface of the heated glass The heat is turned off instrument pulled away from a circular cutting edge Product MF 1 115 Microforge without Optics 115 VAC 60 Hz MF 1 230 Microforge without Optics 230 VAC 50 Hz Replacement Parts l BS4 69 0385 MF 2 Electrode Filament Holder Vertical 7 6 mm 0 3 in Horizontal 15 2 mm 0 6 in BS4 69 0387 MF 4 Hammering Rod SS 3 16 x 3 75 in Focus 12 7 mm 0 5 in 95 mm D x L Weight 17 25 kg 38 Ibs BS4 69 0388 MF 5 Bulb Frosted for Microforge Illuminator Dimensions HxWxD 38 1 x 27 9 x 40 6 cm 15 x 11 x 16 in without optics BS4 69 0389 MF 6 Tool Holder Clamp Power 100 120 VAC 60 Hz 220 240 VAC 50 Hz BS4 69 0390 MF 7 Fuse ACG 5 1 4 x 1 1 4 in BS4 69 0391 MF 8 Filament Wire Platinum 20 IRID 102 Harvard Apparatus now mm 4 in L 31 ga 229 um 0 009 in OD owns Clark Electromedical BS4 69 0392 MF 9 Filament Wire Platinum 20 IRID 102 See pages M4
90. ally limited the number of samples and cell types they could test are all about to change The Methods Optimization Systems MOS 25 amp MOS 96 allow you to boost your productivity by optimizing your conditions and increasing your yields and viability The MOS 25 system takes less then 5 min utes to run while the MOS 96 less then 10 minutes These systems allow the user to quickly and efficiently optimize the electrical and biological parameters associated with any electroporation experi ment Now entire libraries can be screened rapidly and efficiently Both the MOS 25 and MOS 96 Advanced Electroporation Systems come complete with disposable multi well Optimizer Plates a Plate Handler and an ECM 830 Generator see pages M56 and M57 for complete details on the generator U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Catalog No BS4 45 0410 BS4 45 0411 BS4 45 0420 BS4 45 0421 BS4 45 0452 BS4 45 0462 BS4 45 0401 BS4 45 0400 BS4 45 0002 BS4 45 0052 BS4 45 0001 BS4 45 0051 Model MOS 25 200 MOS 25 100 MOS 96 200 MOS 96 100 HT P96 4 HT P25 4 HT 200 HT 100 8300 ECM830 6300 ECM630 Description MOS 25 200 System includes ECM 830 Generator 6 x 25 Well Optimizer Plates HT 200 Plate Handler and instructions MOS 25 100 System includes ECM 830 Generator 6 x 25 Well Optimizer Plates HT 100 Plate Handler and instructions
91. alters the potential energy of the cadmium electrons enhancing the rate of their transfer to nitrate Even though this design has been used successfully for many years it has two main drawbacks The loss of its catalytic activity after using it on a number of samples requires that the reductor be reactivated The second problem deals with the difficulty in handling and utilizing the beads for use with very small sample volume containers to avoid diluting the sample because these beads are normally packed in a column Nitric Oxide Our nitrate reducing wire is based on a multi metal alloy structure This multi metallic structure preserves the activity of the reductor and enhances the rate of elec tron transfer in these chemical reactions M gt Mt 2e NO 2e H 0 gt NO 2 0H This alloy structure completely eliminates the need to ever reactivate the nitrate reductor The design of the reductor in the form of a wire enables it to be used on very small sample vol umes and removes the restriction of having to move your sample to a specialized container The reductor is available in two different kit forms The manual kit includes 6 reductors that are used to reduce nitrate in 6 samples simul taneously The motorized kit is a combination of a reductor and a mixer The mixer is the reductor The reductor is the mixer It is ideal for use in sample containers where stirring is difficult The motorized reductor reduces nitrat
92. and narrower bath of the RC 20 insures a linear flow and fast solution exchange while the larger RC 20H provides a larg er viewing area The additional port at the bath input may be used to inject sub stances into the bath or to remove gas bubbles Order with P 5 or PH 5 platform see page M112 Catalog No Model Product BS4 64 0222 RC 20 small Volume Imaging Chamber 48 ul BS4 64 0223 RC 20H small Volume Imaging Chamber 90 ul BS4 64 0276 CR 15 Replacement Retaining Ring Series 20 Imaging Chambers use coverslips at both top and bottom to enclose the bath area The closed bath promotes an even and continuous solution exchange and eliminates changes in focus due to alterations in bath height Studies involving physiological bicarbonate buffers can be performed with no gas loss at the chamber interface The closed design allows the use of per meable supports With a laminar flow minimal distortion of the support will occur Closed cham bers have been used in fluorescent calcium and time lapse imaging studies All series 20 chambers equipped with a stainless steel suction tube now include a suction tube clamp This insures the adjustable tube will not move once the solution height is set For Series 20 Chamber replacement parts see page M117 TOP VIEW Suction Tube Access Secondary Inlet Port Suction Reservoir SIDE VIEW 15 mm Coverslip 15 mm Sample Coverslip BATH DIMENSIONS Volume 48 pl Volume 90 pl l 11 5
93. and regulating the temperature of the perfusing solution Techniques for regulating the temperature of perfusing solutions include preheating the solution reservoir and or using inline solution heaters in the perfusion path way Each strategy has its strengths and weaknesses and are dis cussed below How Temperature is Sensed In general temperature is measured using a thermistor or similar device and fed back into a temperature controller which adjusts power to the heating element When regulating temperature it is important to account for the response time of the feedback loop since this can cause the system to oscillate Control and Regulation Warner Instruments temperature controllers utilize two thermistors per channel to provide monitoring and feedback information to the controller One thermistor is placed within the heated element e g chamber platform in line heater etc to measure the temperature of the heater block Information from this thermistor is fed back to the control mechanism which applies sufficient power to the heater to maintain the controller set point The second thermistor is usually placed in the solution path close to the sample under study and measures the temperature at the sam ple Information from this thermistor is used by the researcher to readjust the set point of the controller and to monitor the perform ance of the system This approach is very effective in providing tight thermal control 0 1
94. ap 1 0 mm Field Type Homogeneous Sample Visibility Yes Minimum Volume 20 ul Maximum Volume 90 ul Catalog No Model Product BS4 45 0207 630B Cuvettes BS4 45 0208 660 Cuvette Rack BS4 45 0125 BS4 45 0126 620 640 Blue Yellow 2 0 mm 4 0 mm Homogeneous Homogeneous Yes Yes 40 ul 80 pl 400 ul 800 ul Cuvette Safety Stand adjustable gap for use with disposable CD Generator Footswitch for ECM 830 The BTX 1250Fs Footswitch is designed to allow hands free operation of the ECM 830 Generator The footswitch functions identical to the Start button on the front panel of the instrument Once all the param eters are dialed in simply press and release the footswitch or Start button to activate pulse delivery or abort a pulse sequence The BTX Generator Footswitch is designed for used with BTX ECM 830 Square Wave Generator Versions 1 08 and beyond have software that supports the footswitch opera tion so no further modification of the gen erator is required If you have a version prior to V1 08 please contact BTX Customer Service and please have the instrument seri al number on the back panel ready to expedite the process Applications The BTX Generator Footswitch is especially useful when conducting in vivo gene delivery in ovo gene delivery and nuclear transfer cloning applications These types of experiments often require the researcher to use both hands for other manipulations gen erating the n
95. around various types and sizes of cells with accuracy to 0 1 micron The process of extruding substances through Pipette Pullers pages M51 and M52 these micropipettes is accomplished through the use of either direct hydrostatic pressure Pressure Injection or by moving charged ions that are the result of an applied electric field lIontophoresis Micromanipulators pages M186 to M201 without the use of hydraulic flow Warner and Harvard Apparatus Clark Capillary Glass pages M45 to M49 Applications of microinjection range from assisted In vitro cell fertil ization techniques to the transport of molecular and cellular elements These substances are typically injected into the cell to manipulate and or monitor the fundamental biochemistry of a specific living cell Substance that can be injected include cellular organelles kinases his tochemical markers such as horseradish peroxidase or lucifer yellow proteins metabolites microbeads ions antibodies genes molecular biology mRNA and DNA etc The precise delivery microperfusion of small volumes picoliter to milliliter of various agents and drugs to a cell or group of cells for applications such as pharmacological drug lesting can also be accomplished using these techniques In order for the researcher to conduct any of the above experiments and get meaningful results the tools equipment used in these experiments must provide not only the specific functionality but they als
96. ate a homogenous field when facing one another The Electrode Adaptor is used to attach the Flat Electrode Chamber to the BTX instrument Applications This Flat Electrode Chamber can be used for both electroporation and electrofusion Product Flat Electrode Divergent or homogeneous field product 484 1 0 mm gap 0 5 ml requires Electrode Adapter BS4 45 0202 and Cable BS4 45 0210 Electrode Adapter product 466 for use with Model 484 Catalog No BS4 45 0108 BS4 45 0202 11 000 Specialty Products to Enhance Your Bioresearch Electroporation Accessories iD Disposabale Electroporation Cuvettes Plus and Safety Stand BIX Cuvettes Plus The BTX Cuvettes Plus are designed for use in electroporation of bacteria yeast mam malian and plant cells Each sterilized Cuvettes Plus package includes a disposable cuvette and a bonus pipette which is used for removal of the sample after electropora tion The cuvettes are molded with embedded aluminum electrodes washed prior to pack aging and gamma irradiated for guaranteed sterility Three electrode gap sizes are avail able 4 mm 2 mm and 1 mm for high field strengths up to 25 0 kV cm Round caps are leak resistant and allow for quick and easy one finger removal BTX disposable Cuvettes Plus require no maintenance Simply discard carefully after use For research use only Specifications Catalog No BS4 45 0124 Model 610 Cap Color Gray G
97. ate chamber insert and an aluminum platform holder The platform clamps the chamber providing a seal between the chamber and coverslip Heater type platforms are used where chamber warming is required The design of the platforms per mits quick removal of the chamber for exchange of the coverslip A variety of bath sizes and shapes are offered to provide a ready solution to many research requirements Most models are stocked in depth for fast delivery Single Coverslip Design A common feature of Series 20 chambers is the use of a disposable coverslip as the bottom or floor of the chamber In many cases this same coverslip will con lain the cells to be studied Thus when used with an inverted microscope the image is viewed through a single 1 coverslip thickness nominally 0 15 mm Laminar Flow Fast Exchange A diamond shape bath is used in many Series 20 chambers models thru RC 26 to provide a linear solution flow Studies performed at Yale and Stanford Universities showed that this shape produced a lami nar flow across the bath The general shape is shown below and specific dimensions are provided in the following pages In general the lower the ratio of bath width W to length L the better the flow character istics Models with small bath volumes can have exchange times measured in seconds even with flow rales less than 1 ml min Newer models starting with RC 27 use a slotted bath shape They are designed for slice stud
98. ators with no resistor settings or unlimited resistance Capacitance Range of 1 uF 25 u F to 3275 uF is the widest capacitance range available for low voltage mode applications Approximately 400 BTX Protocols simplify electroporation steps ECM database with approximately 4000 reference papers give researchers the data needed to develop their own protocols ECM 630 _ The ECM 630 is a state of the art exponential decay wave electropo ration system designed for in vitro and in vivo electroporation appli cations This system incorporates features that make it the most technically advanced exponential decay electroporation system cur rently available The generator utilizes the new BTX Power Platform Technology design and novel digital user interface The revolution ary Precision Pulse System provides the researcher unparalleled power in controlling the time constant With the ability to deliver a maximum of 6000A in the Low Voltage Mode the ECM 630 is the most powerful generator in its class Over 200 ECM 600 protocols may be duplicated with this instrument The additional ECM 630 resistor selection none will allow researchers to reproduce protocols from competitive systems lack ing resistor settings or reporting unlimited resistance Indeed the ECM 630 will perform the widest range of electroporation applica tions among commercial electroporators on the market Application Mammalian Cell Transfections Protein Drug
99. bers are constructed as a new design featuring a three ply solid sandwich construction of stainless steel and mylar plastic Flatpack Chambers are gamma ster ilized in individual packages They are pro vided in sets of 50 and may be used in the Safety Stand along with the Flatpack Slider Applications The Flatpack is used for prokaryotic appli cations It is a unique flow through design with a 0 56 mm gap and a volume from 10 to 85 pl This design provides the unique combination of small sample volumes with field strength as high as 40 kV cm Fase of use and safety features required for the high efficiency transformation of certain gram positive organisms such as E coli Product Flatoack Chambers product 485 1 83 mm Gap 1 5 ml Sterile pkg of 50 Flatpack Chambers product 486 0 56 mm Gap 80 ul Sterile pkg of 50 Catalog No BS4 45 0109 BS4 45 0110 Reference 1 Dr Steven Mayfield Scripps Clinic and Research Foundation Molecular Biology La Jolla Californi CD Flat Electrode The Flat Electrode Chambers are made of two rectangular parallel plates of high grade stainless steel that is press fitted into a poly sulfone base They generate fields depend ing on the orientation of the grooved elec trodes Orientating the grooved sides of each electrode so that they face one another gen erates a divergent field for use in electrofu sion experiments The flat sides of each elec trode will gener
100. bes With this combination a full spectral analysis of dissolved and gaseous oxygen concentration can be achieved The high sensitivity fiber optic spectrometer can be used in a stand alone mode to meas ure fluorescence absorbance reflectance and radiance and other types of standard spectrometric measurements through the use of optional fiber optic accessories Please call our Technical Sales Department for details Dissolved O e Oxygen sensing based on Ruthenium fluorescence detection and fiber optic technology e Does not consume oxygen allowing for continuous contact with the sample e Wide variety of fiber optic sensors sizes available e Compact system combines a high sensitivity soectroohotometer computer interface USB and FO ton Windows 98 NT compatible software e Small size and 12 VDC power makes it ideal for portable and field use e Frequently asked questions sheet available Is the probe influenced by ambient light The probe will be affected by ambient light All probe types should be shielded from ambient light by installation in a closed ves sel or by shielding the probe with a dark housing Our FOXY T1000 probe has a screw on cap to block the optical field of view of the fiber while allowing easy access to the probe tip An overcoat of silicone can be applied to the probe at no charge to help reduce ambient light effects The overcoat slows the response of the probe to 30 50 seco
101. bove BS4 69 0153 see above BS4 69 0150 see page M52 BS4 69 0151 see page M52 BS4 69 0164 and BS4 69 0163 Pulled Pipettes for use with BS4 69 0152 see above BS4 69 0153 see above BS4 69 0160 Rack Mounting Kit with Handles for use with BS4 69 0152 see above BS4 69 0153 see above BS4 69 0150 see page M52 BS4 69 0151 see page M52 BS4 69 0153 and BS4 69 0152 BS4 69 0154 Input Tubing with Connector for use with BS4 69 0152 see above BS4 69 0153 see above BS4 69 0150 see page M52 BS4 69 0151 see page M52 BS4 69 0157 Foot Switch Actuation for use with BS4 69 0152 see above BS4 69 0153 see above BS4 69 0150 see page M52 BS4 69 0151 see page M52 BS4 69 0158 Remote Key Pad to Control for use with BS4 69 0152 see above BS4 69 0153 see above BS4 69 0150 see page M52 BS4 69 0151 see page M52 11 000 Specialty Products to Enhance Your Bioresearch Microinjection amp Microperfusion Nanoject II Auto Nanoliter Injector Micromanipulator and Specifications Stand sold separately Operation Automatic Glass Properties Softening point 780 C Glass Dimensions 1 14 x 0 053 mm 0 045 x 0 021 in OD x ID Injection Speed Fast 46 nl sec Slow 23 nl sec Fill Speed Fast 46 nl sec Slow 23 nl sec Empty Speed 92 nl sec Volume Range 2 3 nl to 69 0 nl Change Volume Dip switches 1 to 4 Change Inject Speed Dip switch 5 Weight 99 2 g 3 5 oz Catalog No Product BS
102. by means of an outlet port of the same gauge The top coverslip is held in place with a retainer and the dish becomes aremm oe mm the chamber bottom A thin coating of silicone grease forms the seal Volume 113 ul Volume 185 pl The distance between top and bottom coverslips is 1 mm The RC Volumes are for 1 mm solution height 37WC uses 15 mm top coverslips and the RC 37FC works with 18 mm coverslips Catalog No Model Product The RC 37WC fits in the Willco Wells dishes while the RC 37FC fits 854 64 0363 RC 37WC Chamber Insert Closed for Willco the Falcon and Corning 35 mm dishes Warner s DH 35 Culture Dish a MM DINGS Heater may be used to heat the Corning and Willco Wells 35 mm B54 64 0364 RC 37FC Chamber Insert Closed for culture dishes Falcon 35 mm dishes are compatible with Warner s PaO goring os Mi Dienes DH 35i Culture Dish Incubator or the QE 1 Quick Change Accessories Replacement Parts Heated Base BS4 64 0703 CS15R Coverslips 15 mm Dia 1 Thickness Box of 100 Both chambers are supplied with PE 160 Polyethylene Tubing p64 64 0384 CS18R Coverslips 18 mm Dia 1 Thickness 10 ft and tools to remove the top coverslip retainer The RC37WC Box of 100 is supplied with one box of 1 thickness 15 mm coverslips and the BS4 64 0758 D3522P Glass Bottom Cell Culture Dishes RC37FC is supplied with one box of 1 thickness 18 mm coverslips pouch of 20 BS4 64 0762 D3522B Glass Bottom Cell Culture Dishes pkg of 120 BS4 6
103. cal recording and or optical imaging and are 1 mm solution height ga lt used in applications including patch clamp oocyte clamp and phys iological measurements on cell cultures and tissue preparations The low profile design permits good electrode access to the bath Catalog No Model Product The RC 40LP 411P and 42LP Chambers use 25 18 and 15 mm B94 64 0367 Rees uier hange Ghamberas mm i Low Profile round coverslips respectively in a round bath design to provide maximum viewing area The chambers work equally well with both 354 64 0368 eae Guies Change Chamber 18 mm Pa eaa Low Profile water and oil immersion objectives BS4 64 0369 RC 42LP Quick Change Chamber 15 mm The RC 40LP 41LP and 42LP Chambers are supplied with one Low Profile package of 1 5 coverslips each 25 18 and 15 mm round Accessories Replacement Parts respectively and spare O rings BS4 64 0375 QE 1 Quick Exchange Heated Base for RC 40 Series BS4 64 0703 CS15R Coverslips 15 mm Dia 1 Box 100 BS4 64 0384 CS18R Coverslips 18 mm Dia 1 Box 100 BS4 64 0705 CS25R Coverslips 25 mm Dia 1 Box 100 BS4 64 0713 CS15R15 Coverslips 15 mm Dia 1 5 Box 100 BS4 64 0714 CS18R15 Coverslips 18 mm Dia 1 5 Box 100 BS4 64 0715 CS25R15 Coverslips 25 mm Dia 1 5 Box 100 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M83 Micro Incubation RC 40 Series Closed Bath and High Profile C
104. chniques either sepa rately or in tandem contact fusion or melting distant or contact stretching fracturing micro forging and microglass blowing Employing a variety of movements and temperature con trols which permit fabrication of microtools from simple to more complex micro instru ments such as calibrated micropipettes microscalpels electrodes and micromanome ters for use in measuring devices for turgor pressure in single cells This microforge is an optical electrical forge system For superior stability the entire optical electrical forge assembly is securely bolted to the cast aluminum main housing This housing contains the transformer function switches Pipette Pullers and the operating controls for the adjusting fil ament temperature and light intensity A circu lar disk or mechanical stage is mounted in front of the lamp housing A pivoting spindle cylindrical vice with clamp for supporting the microtool being made is mounted at the periphery of this disk Both disk and spindle may be rotated about their own axes and preci sion screws can adjust the entire assembly ver tically or horizontally The light intensity direct ed to the microscope objectives may also be adjusted The electrical forge sub assembly is mounted on an adjustable electrode holder sys tem directly in front of the microscope objec tives and to the left of the microtool being fab ricated The spring loaded filament holder provides for
105. cifically designed for this pur pose When an appropriate lamp is used this light does not interfere with the phosphorescence measurements e Two built in light sources one for surface 0 5 to 1 mm and one for deep tissue 2 to 3 cm measurements e Noninvasive oxygen measurements in tissue in vivo or in vitro e Linear relationship between 1 lifetime and pO e Stable over time no sensitivity or baseline drift e Absolute calibration calibrate only once e Windows operating environment e Temporal resolution lt 1 2 sec measurement e Evaluate a wide range of tissue physiology and pathology Vascular and cardiopulmonary function Tumor oxygenation Status of skin grafts Diabetic vascular complications Ischemia reperfusion injury etc e Visible and near infrared phosphors for different tissue depth measurements e Measurements in vitro in sealed glass chambers no oxygen exchange with the environment Types of Phosphors and Phosphor Kits Available Pd meso tetra 4 carboxyphenyl porphyrin This phosphor Oxyphor RO is dissolved in the presence of 0 5 or ereater of bovine serum albumin It provides excellent oxygen sensi tivity and has been accurately calibrated see Lo et al 1996 This phosphor has an absorption band near 524 nm and emission near 690 nm and can be used for surface O measurements in tissues Lyophilized Porphyrin Kit 1 2 g approx amount needed for approx 30 rat rats weighing 30
106. d Glass Catalog No Wire Pellet Connector OD mm Model BS4 64 1075 1 0 QRW A10P BS4 64 1076 12 QRW A12P E ES BS4 64 1077 15 QRW A15P BSA 64 0973 17 QRW A17P pon BS4 64 1078 2 0 QRW A20P BS4 64 1351 Hybrid Straight 1 5 QSH A15N BS4 64 1353 2 0 QSH A20N BS4 64 1354 Yes 14 QSH A15P BS4 64 1355 i7 QSH A17P BS4 64 1356 2 0 QSH A20P Compatible with Axon Patch Clamps and Microelectrode Amps post 2 1 95 NOTE Holders without ports are not suitable for patch clamp recording Q SERIES Holders with 1 mm Pin Threaded Collar Glass Catalog No Wire Pellet Connector OD mm Model BS4 64 1121 Wire Straight 1 0 QSW T10N BS4 64 1122 12 QSW T12N BS4 64 1123 1 5 QSW T15N BS4 64 0749 I7 QSW T17N BS4 64 1124 QSW T20N BS4 64 0837 QSW T10P BS4 64 0838 QSW T12P BS4 64 0839 QSW T15P BS4 64 1097 QSW T17P BS4 64 0840 QSW T20P BS4 64 0857 45 Q45W T10N BS4 64 0858 Q45W T12N BS4 64 0859 Q45W T15N BS4 64 1102 i Q45W T17N BS4 64 0860 Q45W T20N BS4 64 1071 Yes Q45W T10P BS4 64 1072 Q45W T12P BS4 64 1073 Q45W T15P BS4 64 0972 Q45W T17P BS4 64 1074 Q45W T20P BS4 64 0877 l QRW T10N BS4 64 0878 QRW T12N BS4 64 0879 QRW T15N BS4 64 0744 QRW T17N BS4 64 0880 QRW T20N U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M37 ASoj01g 29 Electrode Holders Specialized Tools For Bioresearch M38 Q Series Holders Q SERIES Holders wit
107. d Unique open system with feedback for measuring oxygen consumption of cells cardiomyocytes hepa tocytes endothelial cells Measures down to 0 1 mmHg Two operating modes possible Closed system oxygen consumption calcula ted from pO reduction Open system measurement at from the volume added and the pO 2 he It is an important advantage of the ee ee 5 Sih file ZA method that the pO is maintained of solution with virtually constant during the experi known pO ment In this way the oxygen con e Electrical stimulation sumption can be measured under capabilities steady state conditions at accurately e Fitting for fiber optics for defined pO values while at the same easy Ca concentration or redox potential Port for Oxygen Electrode time also measuring metabolic with Shielding Electrode parameters Port for Fiber Optics QIN E TE CRAG Connecting ports of the Oxystat chamber for a working system it must be equipped with a thermostat the pO measurement electrode the con troller and the pump optionally with fiber optics The stimulation elec trodes are built in Chamber Cover with Stirrer and Grid Stimulation Electrode Main Cell Chamber ASojo1g 2D Lower Inset with Grid Stimulation Electrode and Filter Holder Main parts of Oxystat System Lower inset can easily slide out it receives filter paper and contains lower stimulation electrode Upper cover is clipped
108. d conditioning Sub millimeter sensors are available by special order Specialized Tools For Bioresearch O 0 to 100 PPM 0 to 20 Current Range 0 to 2000 nA Resolution 0 1 PPM 0 1 Accuracy 1 Recorder Output Analog output 0 to 5 V Electrode Response Time Under 10 sec 90 response in stirred solution Display 3 5 digit LCD Power 120 240 VAC Dimensions 20 3 x 22 9 x 76 2 cm 8 x 9 x 3 in Shipping Weight 2 lb 1 5 kg Catalog No Product BS4 69 0026 Oxygen Meter with Temperature Compensation and 2 mm Oxygen Electrode BS4 69 0028 Replacement O Membranes pkg of 4 BS4 72 1920 Magnetic Stirrer 115 VAC BS4 72 1921 Magnetic Stirrer 230 VAC BS4 72 1922 Electrode Holder and Stand M6 11 000 Specialty Products to Enhance Your Bioresearch Dissolved O Phosphorescence 0 Measurement System BS4 69 0502 PMOD 2000 The PMOD 2000 uses a noninvasive optical method for detecting the concentration of O in tissue The operating principle for the unit is based on the quenching of phosphorescence by oxygen in phospho rs that have been injected into the blood To determine the concen tration of O in the area of tissue being measured phosphorescence lifetime measurements are taken using a sinusoidally modulated light source for excitation and frequency lock for phosphorescence detec tion The PMOD operates over a frequency range from 200 to 20 000 Hz allowing measurements of phosphorescence lifetimes from lt 1
109. d firmly in the set position Holder head and coupler are made from Delrin The anodized aluminum handle is 6 3 mm diameter x 6 3 cm long and will fit most positioners MHH 38 and MHH 25 ASoj01g 129 Device Compatibility Catalog No Description BS4 64 0218 MHH 25 E and Q Series Electrode Holders BS4 64 0219 MHH 38 PE Series and Theta Electrode Holders MP and MPP Series Manifolds A Diameter Diameter MHH 25 6 3 mm 1 4 MHH 38 9 5 mm 3 8 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M43 Electrode Holders Electrode Manifold Holders MHH 25 MHH 38 Microelectrode Holder and Replacement Parts Catalog No Qty Pkg Description Coupling Elements BS4 64 1297 3 SP 1M Molded Ag AgCl pellet assembly C TE BS4 64 1282 10 AG25 10 Ag wire 0 25 mm diameter x 70 mm long SP 1M BS4 64 1288 1 HP 205 Hybrid Ag AgCl pellet Ag wire assembly AG 25 Pipette and Wire Seals BS4 64 1289 10 PS 10 Pipette seal for 1 0 mm diameter glass BS4 64 1290 10 PS 12 Pipette seal for 1 2 mm diameter glass BS4 64 1291 10 PS 15 Pipette seal for 1 5 mm diameter glass 6 G6 BS4 64 1374 10 PS 17 Pipette seal for 1 7 mm diameter glass PS xx WS 1 BS4 64 1292 10 PS 20 Pipette seal for 2 0 mm diameter glass BS4 64 1298 10 WS 1 Wire seal for E ME MP and theta holder BS4 64 1299 10 WS 2 Wire seal for Q and PE Series holders WS 3 BS4 64 1300 10 WS 3 Wire seal for
110. de for in vivo skeletal muscle work as well as subcutaneous tumor electrochemotherapy applications Caliper Electrodes can potentially be used for intact plant work as well Caliper Electrodes complement BTX Tweezertrodes Model 520 522 BTX Genetrodes Model 508 S and 510 S and BTX 2 Needle Array Electrodes Model 530 531 532 533 as part of the BTX in vivo electrode line Caliper Electrodes can be used to reproduce applications requiring tweezertrodes transdermal and Genetrodes 2 Needle Array electrodes in vivo muscle This product meets the requirements of the European Communities EC and complies with EMC Directive 89 336 EEC and 73 23 EEC for product safety Voltage Range 0 to 500 V depending on electrode gap Pulse Length Range Model 384 Electrode Dimensions Electrode Material Model 384L Electrode Dimensions Electrode Material 1 usec to 99 msec multiple pulsing permitted 1 0 x 1 0 cm brass 1 5 cm x 1 5 cm 2 0 cm x 2 0 cm spare pair included stainless steel Generator Compatibility ECM 830 600 630 T 820 Electrode gap 0 1 to 13 cm Catalog No Model Product BS4 45 0101 384 1 0 x 1 0 cm brass end plates BS4 45 0102 384L 1 5 x 1 5 cm stainless steel end plates References 1 Zhang Lei et al Biochemical and Biophysical Research Communications 220 633 635 1996 2 Mir et al PNAS 96 4262 4267 1999 3 Mir etal British Journal of Cancer 77 12 2336 2342 1998 4 Dev et al US Paten
111. e saving money saving dispensing bottle DURACAL Buffers are guaranteed for 5 years from the date of produc tion During this time they retain a high buffer capacity for fast stable calibrations The pH 7 00 buffer is less sensitive to ions while the pH L 9 21 buffer provides stability superior to conventional buffers See Figure 1 for a comparison of j N 5 year buffer DURACAL and conventional buffer stability when subjected to prolonged air exposure e Unique time saving CALPACK The unique easy to use CALPACK dispensing bottle allow electrode calibration in the buffer bot dosing bottle tle without contamination In 4 easy steps calibration is complete see Figure 2 It s that simple No more searching for suitable No more searching for a suitable container no more cleaning no more risk of contamination containers and no more wasted buffer from overfilling a container The composition of DURACAL Buffers has been optimized so the temperature function is similar to that of conventional buffers This guarantees that the automatic buffer recognition of most pH meters can be used without any e Economical and easy to dispense problems only the required quantity of buffer for calibration no wasted buffer No more cleaning No more risk of contamination Duracal Buffers e Automatic buffer recognition with a majority of pH meters Specifications pH 7 00 pH 9 21 pH 10 01 Redox 475 mV Certified traceab
112. e as the pulling force rather than gravity or magnetic fields used by other manufacturers This technique provides more controlled even and consistent dragging characteristics allowing the units to pull an ideal injection tip or microelectrode tip easi ly A precision micro linear ball bearing rail and advanced pneumatic components are used to provide no fault pulling movement Programming A simple 4x4 keypad and full information dis play allows the user to easily control and read all pulling parameters Programming functions include sequences steps time timing heater level heater control and tip length and actions These units are supplied with input tubing and connector spare heater filament tweezers power cord and manual Pulling Force Pneumatic Multi Barrel Pipette 4 or 7 barrel BS4 69 0150 only Each Barrel Pipette 1 mm OD Heater Nichrome coil Heater Control Microcontroller Heating 74 general heat levels 24 to 99 54 auto heat levels 45 to 98 Number of Sequences 25 Steps in Each Sequence 18 Taper Length Setting 0 5 to 20 mm Pressure 1 Regulator 0 1 to 10 p s i Pressure 2 Regulator 0 1 to 60 p sii Pressure Gas Input 30 to 60 p s i Actions Pull 1 pull 2 pull 2 cool rotation BS4 69 0150 only cool air return Display 20 x 4LCD Power Input 110 240 VAC Power Consumption Max 150 W Dimension HxWxD 17 8 x 27 9 x 35 6 cm Weight BS4 69 0150 6 8 kg 18 lbs BS4 69 0151 5 9 kg 13 Ibs Catal
113. e electrode itself has been used for the transfection of 3T3 L1 rat adipose cells SF295 human neuroglioblastoma and Cos 7 cells Plant Yeast and Bacterial Electroporation Applications Applications involving plant yeast and bacterial electroporation may be carried out using the Petri Pulser Scale up and multiple sample processing This product meets the requirements of the European Communities EC and com plies with EMC Directive 89 336 EEC and 73 23 EEC for product safety Voltage Range 0 to 300 V Electrode Thickness 0 5 mm Pulse Length Range 1 to 35 msec in PBS Electrode Material Gold Volume Range 0 5 to 3 0 ml Generator Compatibility ECM 830 630 399 Weight 6 oz Gap Size 2mm Number of Electrodes 13 Catalog No Model Product BS4 45 0130 PP35 2P Petri Pulser Electrode References 1 Marrero et al Journal of Biological Chemistry 270 26 15734 15738 1995 2 Scheiffer et al Journal of Biological Chemistry 271 17 10329 10333 1996 3 Marrero et al Journal of Biological Chemistry 272 39 24684 24690 1997 4 Fukai Masuko Emory University Personal Communication 1998 5 Fukai Masuko Emory University Personal Communication 1998 6 BTX Protocols PRO165 and PRO330 1998 7 BTX Protocols PRO290 PROO286 PRO359 PRO360 PRO364 1998 Gs Microslides The BTX Microslides are used primarily with the ECM 2001 Electro Cell Manipulator The Microslide fits on the microscope allowing observat
114. e faster than the manual kit version The motorized version has a removable reductor and comes with a micro sleeve enabling the user to utilize it as a micro mixer in the lab Catalog No Product BS4 69 0054 Manual Nitrate Reductor Kit pkg of 6 BS4 69 0055 Motorized Nitrate Reductor Kit U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 2D MS Dissolved O C Dissolved Oxygen Meter and Electrodes BS4 69 0026 Dissolved Oxygen Meter shown with 2 mm Oxygen Electrode Stirrer and Electrode Stand not included 70mm 2 mm Diameter Cable and Connector 3 5mm x 6 ft D x L Handle 6 4 x 50 mm D x L Tip Stainless steel 2 x 70 mm D x L with flat sensing tip Cat No Product BS4 69 0027 Oxygen Electrode 2 mm BS4 69 0028 Replacement O Membranes pkg of 4 This dissolved oxygen measuring system uti For more Oxygen Detection lized the latest technologies in electronic cir Systems see pages M7 M 14 cuitry and chemical sensors Using the 2 mm stainless steel tip electrode oxygen can be measured in either liquid solution or gas phase The BS4 69 0027 2 mm stainless steel elec trode see to the right is supplied with each sys tem with two replacement membranes and electrolyte The meter can display current per centage or PPM oxygen The meter offers an activation function to restore the activity of the sen sor without the need of polishing an
115. e in patch experiments Composition 68 SiO 18 B O 9 K O 3 Al O 1 Li O 1 Na O Softening Temperature 718 C Dielectric Constant 5 7 Premium Corning 7056 Patch Glass Cat No Model OD mm IDmm Length BS4 64 0812 G75150S 4 1 50 0 75 100 mm 225 BS4 64 0813 G75150T 4 1 50 1 10 100 mm 225 BS4 64 0814 G75165T 4 1 65 1 20 100 mm 190 Premium Corning 8161 Potash Rubium Lead Patch Glass a This glass pulls at a lower temperature and is easily shaped It is recommended for low noise recordings However the lead content should be considered for the intended application Specifications Composition 51 PbO 39 SiO 6 K O 2 BaO Softening Temperature 600 C Dielectric Constant 8 3 Premium Corning 8161 Potash Rubium Lead Patch Glass Model OD mm IDmm Length Qty G86150T 4 1 50 1 10 100 mm 225 G86165T 4 1 65 1 20 100 mm 190 Cat No BS4 64 0815 BS4 64 0816 For Patch Clamps see pages H2 to H5 11 000 Specialty Products to Enhance Your Bioresearch Capillary Glass Clark Capillary Glass Discounts on Quantity Puchases Discount apply to only single part number quantities no mixing 1to4 none Clark Electromedical Instruments was acquired by Harvard Apparatus Lid Harvard Apparatus UK Sto9 5 Harvard Apparatus Ltd Acquires Clark Electromedical Instruments subsidiary located in Edenbridge England Clark Electromedical Instruments manufactures m
116. e times are needed to ablate the zona resulting in increased embryo viability The ZonaDrill is the safest laser on the market today and is 100 accurate guaranteed The Unique Safety Circles based on independ ent medical research gives the user a visual indication of the Safety Zone for the protection of critical cells This laser system features a unique hand held control a pilot laser targeting system and a digital ly controlled pulse time Additionally the ZonaDrill requires no spe cial needles pipettes and is portable between microscopes Use ZonaDrill for trouble free Cell Nuclear Transfer ICSI and stem cell removal ZonaDrill can also be used in conjunction with fluorescence Available with the Cronus software suite for cell embryo analysis and digital target generator Ultra Focused Laser Beam Solid State Diode Laser 1480 nm 300 mW Power Density at Focus 4 5 mWicm Laser Focus Diameter 2 um Non Mercury Pilot Laser 635 nm Spot Targeting Solid State Diode Laser Objective Lens 40X Catalog No Product BS4 72 6475 ZonaDrill for Olympus 1X50 1X70 BS4 72 6476 ZonaDrill for Olympus 1X51 1X71 1X81 BS4 72 6477 ZonaDrill for Nikon Eclipse TE 200 300 BS4 72 6478 ZonaDrill for Nikon Eclipse TE 2000 BS4 72 6479 ZonaDrill for Nikon Diaphot 200 300 BS4 72 6480 ZonaDrill for Zeiss Axiovert 200 BS4 72 6481 ZonaDrill for Zeiss Axiovert 100 135 BS4 72 6482 ZonaDrill for Leica DMIRB
117. e which is attached to the electrode and sealed to pre vent salt contamination a sleeve to use the electrode in our chamber system a sleeve to use the electrode outside the chamber extra membrane material and o rings a membrane installation tool and polishing paper and Scotch Brite for maintaining the electrode This electrode is compatible with the BS4 56 5077 amplifier and YSI s model 5300 oxygen monitor the cables are different so specify the appropriate part number We also supply a special Thurman style electrode for existing liver perfusion set ups Product Standard Electrode with Cable Standard Electrode with YSI Cable Replaces YSI Part Numbers 5357 and 5358 Thurman Style Electrode Catalog No BS4 56 5002 BS4 61 0134 BS4 61 0136 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M13 Dissolved O Harvard Instech Biological Oxygen Monitoring Systems Specialized Tools For Bioresearch M14 Batch Flow Chamber System Each chamber system consists of an anodized aluminum chamber block with connections for circulating water to keep the electrode and sample at a constant temperature The chamber can be set up for oxy gen measurement in a fixed 600 pl sample by inserting the Kel F batch cell cup into the chamber block The batch cell chamber has separate ports for filling overflow and additions made with a micropipette or syringe A magnet
118. eed for footswitch activation The BTX Generator Footswitch complies with EC Safety Directive EN61010 1 1993 A2 95 for product safety Dimensions HxWxD 2x4 5x3 5in Weight lt 2 b Cord Length 10 ft Model Product 1250S Catalog No BS4 45 0211 Footswitch for ECM 830 Generator U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 2D M69 Microinjection amp Microperfusion Introduction to Microinjection The use of based techniques for intracellular extracellular microinjection and perfusion has become a popular procedure in numerous areas of exper imental biology research e g In vitro fertilization transgenics etc These techniques can best be described as micro surgical procedures that are conducted on a single cell using either a single or multiple barrel glass micropipette a precision positioning device micromanipulator and a microinjector or microperfusor The micropipettes used in these procedures are formed using a Pipette Puller The capillary glass tubing is heated to its softening point and pulled to create the proper size tip diameter and taper for the desired application The small tip diameters as low as 0 2 um of these micropipettes combined with the high precision of the Micromanipulator and allows for precise and accurate delivery This precision allows for accurate and repeatable injections down to the sub picoliter liter range into or
119. electroporation pulse lengths The ECM 2001 is the only square wave electroporation system commercially available that allows this range of pulse lengths The researcher can optimize the experimental conditions to obtain the best results possible Post fusion AC pulse allows for compression of the cells following application of the electrofusion pulse Hybrids do not fall apart resulting in a higher number of hybrids The manual start allows for real time visual optimization to obtain maximum number of hybrids Best results are obtained for fusing blastomere to oocyte for embryo cloning The automatic start allows the researcher to start the align ment fusion and compression process by pressing a single button A remote control allows easy operation of the instrument from outside the hood while the number of repeats allows for expansion of parameters U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Specifications AC Parameters Alignment Proprietary Non Sinusoidal Wave Shape Frequency 1 MHz Voltage zero to peak 0 to 75 V Duration 0 to 99 sec Post Fusion AC 1 10 of prefusion DC Pulse Parameters Fusion Electroporation High Voltage Mode Voltage 10 to 3000 V Pulse Length 1 to 99 usec Low Voltage Mode Voltage 10 to 500 V Pulse Length 0 01 to 0 99 msec General Amplitude 1 to 99 msec Post Fusion Ramp 1 to 9 sec Pause between AC DC 50 usec Number of Pulses 1 to 99 Sequence Auto
120. elial Studies f The RC 50 is a low profile hori zontally mounted Ussing cham ber incorporating special fea tures for using confocal imag ing The chamber can be config ured as a closed perfusion sys tem using either gravity or pump feed The apical upper surface of the chamber can be used in an open or closed configuration The basolateral chamber has been designed to facilitate place ment and stretching of hard to handle tissue such as trachea Suture pins are provided to act as anchor points for suture lines A 1 glass coverslip forms both the top and bottom of the chamber allowing it to be used with both inverted and upright microscopes While the chamber bottom is beveled to permit maximum access to the bottom coverslip the chamber body can also be inverted to allow access to the other side The viewing aperture in the chamber baso lateral section is 17 7 mm in diameter and the viewing aperture in the chamber apical section is 22 8 mm The RC 50 includes a pair of resistive heater elements mounted to the chamber base Heater elements are compatible with Warner TC 324B and TC 344B Heater Controllers Specifications 12 4 mm H x 82 mm D lt 110 g assembled with heaters Physical Dimensions Weight Platform and Pressure Plate Aluminum black anodized Coverslip Plates Polycarbonate PE 90 Top 25 mm Bottom 22 x 40 mm Input Output Tubing Coverslips 1 Pressure Plate Wrench Pressure Plate Cov
121. enepaddles causes transient pores to form in the cells of the tissue allowing uptake of the molecules into the cells Itasaki used a 3 x 5 mm Genepaddle style electrode with a BTX Generator to deliver DNA to mouse embryo in vitro His success demonstrates the potential of electroporation to transfect different types of tissues or embryos besides the classical avian embryo ASojolg 129 In Vivio Gene Delivery Paddle style electrodes have also been used for in vivo applications Miyasaka used an electrode of this style with a BTX Generator to deliver DNA in vivo into the cerebral corical anlage of E15 rat embryos He demonstrated high efficiency gene transfer into intact neural tissue using electroporation Electroporation has the benefits of being less labor intensive and more economical than competitive methods for in vivo gene transfer such as viral methods and biolis tics Electroporation also produces greater viability and efficiency than competitive methods for in vivo gene transfer References 1 Ttasaki et al Shocking developments in chick embryology electroporation and in ovo gene expression Nature Cell Biology 1 E203 E207 1999 2 Miyasaka et al Foreign gene expression in an organotypic culture of corical Call f or your local B TX Dealer anlage after in vivo electroporation NeuroReport 10 2319 2323 1999 or v i S it WWW b txon lin e com U S Toll Free 800 272 2775 Fax 508 429 5732
122. er allows air to escape when the holder is inserted into the cell It may also be used to introduce other solutions during the course of a respiration run and a fine bore needle with luer fitting included A PTFE covered magnetic spinbar is provided and the cell should be located on a magnetic stirrer unit not provided For fast reactions it is recommended that FEP mem branes used with electrode jacket BS4 69 3011 are used e Good visibility of cell contents Model RC 300 RC 350 Cell Diameter 13 mm 16 mm Cell Volume 0 3 to 1 0 ml 1 0 to 3 0 ml Cell Glass Electrode Holder Black acetal Catalog No Model Product BS4 69 3018 RC 300 Respiration Cell BS4 69 3019 RC 350 Respiration Cell BS4 69 3027 Replacement Glass for BS4 69 3018 BS4 69 3028 Replacement Glass for BS4 69 3019 BS4 69 3032 Spinal Needles for use with BS4 69 3018 amp BS4 69 3019 pkg of 3 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 129 M21 Respirometry m Specialized Tools For Bioresearch M22 CD MicroCell Model MC 100 a For pO measurement of blood or other fluids or for micro flowcell use e Small Sample Volume e Flow through or spot measurement e Exceptional visibility e Easy acess for cleaning The all glass sample chamber has a capacity of approximately 70pl Samples are injected into the chamber through a 1mm stain less steel tube fitted with a fine pv c
123. er and 1 2 mm inner diameter pre pulled to 2 mm formation as shown The design of the micropipette readily lends itself to electrical connection by inserting Ag wire or to pressure tube attachment for pneumatic use The overall length of the blank assembly is 10 centimeters The micropipette is supplied as either pre pulled blanks or fully pulled to a micron tip as shown The micron tip is not bumped and therefore can be finalized prior to use Features e The orifice ratio of each barrel is 0 80 and thus is optimized for minimal tip resistance when pulled to micron size e Omegadot tubing permits easy filling e Fanned out outer barrel assembly provides separation thus avoiding leakage due to spills and any cross coupling between barrels e The 22 mm long twisted segment of the blank assembly assures singular tip formation during pulling action thus provides flexibility in a selection of a desired tip configuration Cat No Model Micropipettes BS4 65 0207 MS 7PB Micropipette 7 Barrel 1 5 mm OD Borosilicate Glass Pre Pulled Blank pkg of 20 BS4 65 0208 MS 7MT Micropipette 7 Barrel 1 5 mm OD Borosilicate Glass Fully Pulled approx 20 um Tip pkg of 10 For the Neuro Phore BH 2 System for iontophoretic applications see pages L4 to L8 11 000 Specialty Products to Enhance Your Bioresearch Pipette Pullers Introduction to Pipettes and Pullers Needle micropipette fabrication devices include instruments in
124. eries 20 Open Bath Recording Imaging Chambers RC 26 RC 26G TOP VIEW Suction Well Perfusion Inlet Port Agar Bridge Well SIDE VIEW Suction Tube Slot gp BATH DIMENSIONS RC 26G 15mm ee Volume 234 pl gt 25mm Volume 170 pl Volumes are for 1 mm solution height Large Bath Recording Chamber RC 26 170 pl amp RC 26G 234 pl These chambers accommodate larger slices and preparations and permit excellent access for electrodes The larger baths also allow for insertion of a round coverslip from the top a 12 mm in RC 26 and a 15 mm in RC 26G Optional slice hold down anchors are offered for both models The anchors are stainless steel frames with lycra threads at 2 mm spacing and weigh approximately 500 milligrams Both models use a 22 x 40 mm cov erslip on the bottom Order with P 1 or PH 1 platform on page M112 Catalog No Model Product BS4 64 0234 RC 26 Large Bath Recording Chamber BS4 64 0235 RC 26G Large Bath Recording Chamber Optional Slice Anchors BS4 64 0252 SHD 26H 2 Slice Anchor for RC 26 2 mm BS4 64 0255 SHD 26GH2_ Slice Anchor for RC 26G and RC 26GLP 2 mm U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com RC 26GLP TOP VIEW Perfusion Inlet Port Agar Bridge Well Suction Tube Slot SIDE VIEW 20 mm 2 mm i l T BATH DIMENSIONS N OT DSH z Volume 234 pl A Volumes are for 1 mm solution height nv Low Pr
125. erslip Retaining Ring Port Suture Pins ae Basolateral Chamber Platform Catalog No BS4 64 0605 Optional Accessories BS4 64 0322 BS4 64 0323 BS4 64 0324 BS4 64 0325 BS4 64 0326 BS4 64 0327 BS4 64 0326 BS4 64 0328 BS4 64 0329 Model RC 50 SA 30NIK SA 30TMS8 SA 30TMS9 SA 30 OLY2 SA 30LZ SA 30KZ SA 30LZ SA 30L3P SA 30PLI Top Coverslip 25 mm Apical Chamber Basolateral Perfusion Port Bottom Coverslip 22 x 40 mm Product Confocal Imaging Chamber Stage Adapters for Nikon Diaphot TE200 TE300 Stage Adapters for Nikon TMS with 8 x 12 cm Cutout Stage Adapters for Nikon TMS with 9 x 13 cm Cutout Stage Adapters for Olympus IMT 2 1X50 1X70 BX50WI Stage Adapters for Zeiss Axiovert 211 x 230 Specimen Stage Stage Adapters for Zeiss Axiovert 85 x 130 Mechanical K Stage Stage Adapters for Leica DMIRB E with Plane Stage Stage Adapters for Leica DMIRB E with 3 Plate Mechanical Stage For Prior amp Ludl Motorized Stage U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASojolg 129 M95 Special Chambers Systems Harvard Medical Systems Microscope Stage Incubators Specialized Tools For Bioresearch M96 BS4 65 0101 CSMI Micro Incubator CSMI Chambered Slide Micro Incubator The CSMI is a versatile micro scope stage incubator for cell tissue culture work It is designed to accommodate new rectangular disposable
126. escence 0 Measurement System The Cool Colors LED lamps are specifically designed for this pur pose When an appropriate lamp is used this light does not interfere with the phosphorescence measurements The Windows compatible software included with the PMOD 2000 sys tem is used to configure the system s parameters and to display and store the measurement frequency signal amplitude phase and time of measurement as well as the oxygen pressure The time course of phosphorescence lifetime or oxygen pressure can be continuously plotted on screen as the data are taken All of the displayed signal parameters can be saved to named files for further analysis etc The files are in ASCII and are easily read by other programs such as Excel Sigma Plot etc Systems come complete with a 6 mm OD bifurcated optical fiber cable Other optical fiber configurations are also available please call our technical sales department for addi tional information Computer Requirements The operating software is for DOS or Windows 95 98 NT and requires an IBM compatible PC Only the Windows NT version will allow determination of the distribution of phosphorescence lifetimes In any case communication with the computer requires a 16550 UART serial port or later Method Reference Publications D Vanderkooi JM Maniara G Green TJ and Wilson D E An optical method for meas urement of dioxygen concentration based on quenching of phosphorescence
127. et al used Tweezertrodes to deliver a Green Fluorescent Protein GFP gene into rat liver The authors demonstrated success ful gene transfer and expression in situ Tweezertrodes complement BTX Models 508 510 Caliper Electrode Models 384 and 384L 2 Needle Array Models 530 and 532 as the newest addition to our in vivo electrode line The Tweezertrodes can be used to reproduce applications requiring the caliper electrodes including gene therapy transdermal drug delivery and electropora tion therapy ASojolg 129 References 1 Suzuki et al Direct Gene Transfer into Rat Liver Cells by in vivo Electroporation FEBS Letters 425 436 440 1998 2 BTX Electroporation Protocol PRO363 1998 3 Zhang et al In vivo transdermal delivery of large molecules by pressure mediated elec troincorporation and electroporation a novel method for drug and gene delivery Bioelectrochemistry and Bioenergetics 42 283 292 1997 4 Dev Giordano and Brown In vivo delivery of gene to rabbit carotid artery by electro poration Third US Japan Symposium on Drug Delivery Systems Abstr 38 1995 5 Dev Killing cancer cells with a combination of pulsed electric fields and chemothera peutic agents Cancer Watch 3 12 14 1994 6 BTX Electroporation Therapy Protocols ECT 001 ECT 005 1995 Call for your local BTX Dealer or visit www btxonline com U S Toll Free 800 272 2775 Fax 508 429 5732 Online www har
128. f Power and Start switches R Pia o l l Monitoring Monitoring and display of Vp and RC Time Constant 1 Bodwell et al Journal of Steroid Biochemistry and Molecular Biology 68 77 92 1999 Warranty 2 Years 2 Ishmael et al Journal of Neurochemistry 67 4 1500 1510 1996 3 Sundback et al Journal of of Immunology 5971 5978 1998 Catalog No Model Product 4 B t al l of Bacteriology 177 17 4836 4840 1995 ete ss ee BS4 45 0051 ECM630 ECM 630 Generator Only 5 Sanders et al FEMS Microbiology Letters 154 2 251 258 1997 6 Faber et al Current Genetics 25 4 305 310 1994 BS4 45 0001 6300 ECM 630 Electroporation System 7 Lin etal Plant Physiology and Biochemistry 35 12 959 968 1997 8 Smith et al Plant Science 104 49 58 1994 9 Chen et al Zoological Studies 34 4 215 234 1995 10 Yee and Nash T E PNAS 92 12 5615 5619 1995 11 Quillan et al PNAS 92 2894 2898 1995 12 Peterfy et al methods in Molecular and Cellular Biology 5 6 353 362 1995 13 Parham et al Cytotechnology 28 1 9 1999 14 Cui Journal of the American College of Cardiology 29 201a 1997 15 Zhang et al Bioelectrochemistry and Bioenergetics 42 283 292 1997 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Includes ECM 630 Generator BS4 45 0051 Cuvettes 1 mm 2 mm 4 mm pack of 30 10 each Cuvette Safety Stand BS4 45 0207 and Cu
129. f the traces during recording and the instruction to print the recording screen to printer if hard copy is required are all entered Many of these will not change from one experiment to the next and it is only necessary to enter changes Recording Clicking the Start button initiates the recording process and traces then scroll across the screen If substrates inhibitors or other solu tions require to be added during the run the position where this occurs can be recorded with a flag and the nature of the addition is recorded on a drop down menu At the termination of the experi ment the data are saved to a data file and may be analyzed straight away if required Analysis The data file recorded traces and event marker flags are recalled to the screen If the recording has exceeded one screen width it can be contracted to fit the screen if required Alternatively it is possible to scroll along long recording traces Selector lines may be dragged to enclose specific parts of the trace Thus if the traces show both con trol respiration and changed respiration after adding a solution the selector lines would be dragged to the control rate first and then to the changed rates Respirometry In each case respiration rates normalized if so designated are cal culated automatically by clicking the Calculate button The calculat ed rates are tabulated together with the experimental details entered during Setup on the
130. fer compo sition This product meets the requirements of the European Communities EC and complies with EMC Directive 89 336 EEC and 73 23 EEC for product safety Reference 1 C Baum et al An Optimized Electroporation Protocol Applicable to a Wide Range of Cell Lines BioTechniques vol 17 6 1058 1062 1994 Call for your local BTX Dealer or visit www btxonline com Specifications Operational Status Interface Charge Time Voltage Range LV Mode HV Mode Capacitance LV Mode HV Mode Resistance LV Mode HV Mode Safety Dimensions H x W x D Weight Display Controls Monitoring Warranty Catalog No BS4 45 0050 BS4 45 0000 BS4 45 0212 Internal Self Test Upon Start up Digital User Interface 5 sec maximum 2 to 500 V 2 V resolution 10 to 2 500 V 10 V resolution 1050 pF 36 pF 150 Q 150 Q Short circuit and arc proof 10 9 x 23 1 x 19 6 4 3 x 9 1 x 7 7 in 7 b 16 character LCD backlit Single rotary encoder for voltage selection Additional on off power and start switches Monitoring and display of Vp and RC time con stant 2 Years Product ECM 399 Generator Only 399 ECM 399 Electroporation system Includes ECM 399 Generator BS4 45 0050 Cuvettes 1 mm 2mm 4 mm pack of 30 10 each PEP BLU BS4 45 0212 and Cuvette Rack BS4 45 0208 Personal Electroporation Pak plug in cuvette module Model ECM399 PEP BLU 11 000 Specialty Products
131. filling is ever required reference solution can be injection into the side of the tee fitting and flexible tube portion of the electrode Product Flexible Reference Electrode Catalog No BS4 69 0490 11 000 Specialty Products to Enhance Your Bioresearch Reference Electrodes Auto Chloridizer 863 Kit The electrolytic chloridation unit Auto Chloridizer 863 provides a simple means for the chloridation of Ag AgCl electrodes It covers the surface of the silver electrode with an AgCl film insoluble in water Unlike a purely chemical procedure electrolytic chloridation does not involve the use of substances endanger ing the environment It is normal practice to work with physiological saline solution NaCl as the electrolyte The electrolytic chloridation method also offers excellent reproducibility of the AgCl film at constant current density and chloridation time The auto chloridizer operates with an electronically controlled constant current source with current monitoring and error indication through an LED and error beep During the chloridation of a single Ag AgCl electrode the unit is operated manually through the start stop key For routine production of several identical electrodes such as Ag AgCl wires for glass microelectrodes the unit offers a simple programming feature for the chloridation period in the repeat mode First the learning mode is initiated by pressing the repeat key for longer than 1 second and then
132. g Initial tests showed the 8520 glass to be equal to the 7052 in noise performance More importantly seals were formed faster and maintained for longer periods gt 10 SiO gt 10 B O gt 1 Al 0 gt 1 K O lt 1 Na O lt 1 Li O lt 1 ZnO lt 1 As O lt 1 TiO lt 1 ZrO 720 C Dielectric Constant 4 6 Composition Softening Temperature Premium Custom 8520 Patch Glass Cat No Model OD mm IiDmm Length BS4 64 0817 G85150T 3 1 50 1 16 75mm 225 BS4 64 0818 G85150T 4 1 50 1 16 100 mm 225 BS4 64 0819 G85165T 3 1 65 1 28 75mm 190 BS4 64 0820 G85165T 4 1 65 1 28 100 mm 190 Clark Custom 8520 Patch Glass Catalog No OD Quantity BS4 30 0091 1 2mm 0 93 mm 75 mm 350 BS4 30 0089 1 2mm 0 93 mm 100mm 350 BS4 30 0090 1 2mm 0 93 mm 150mm 350 BS4 30 0094 15mm 1 16 mm 75 mm 225 BS4 30 0092 15mm 1 16 mm 100mm 225 BS4 30 0093 i5 mm 1 16 mm 150mm 225 BS4 30 0097 1 65mm_ 1 28 mm 75mm 190 BS4 30 0095 1 65mm_ 1 28 mm 100mm 190 BS4 30 0096 1 65mm 1 28 mm 150mm 190 Premium Corning 7056 Alkali Barium Borosilicate Patch Glass O Now Available in Premium Line Corning 7056 glass is now offered in place of the 7052 glass last melted in 1992 and no longer available The 7056 formula has also been discontinued by Corning last melt in 1995 but stocks of this material should be available for a few more years The properties of the 7056 glass are similar to the 7052 as is its performanc
133. g and documentation of parameters e Arc quenching minimizes damage caused by arcing e Generator will not be damaged in the event of a short circuit e External safety stand protects generator from damage caused by spills or splashes e All digital user interface design allows for simple operation e Single rotary dial control simplifies parameter settings e Voltage Range of 5 V to 3000 V is the basis for a generator capable of electroporating ALL cell types e Pulse duration range of 10 us to 10 sec gives unparalleled flexibility for varied applications and optimization range e Footswitch compatible for hands free operation The ECM 830 is a Square Wave Electroporation System designed for all in vitro and in vivo electroporation applications The generator utilizes the BTX Power Platform Technology and a digital user interface The ECM 830 possesses key features including finer voltage discrimination and 0 5 V accuracy Arc Quenching the monitoring of all key parame ters and the control of pulse intervals Applications Mammalian Cell Transfections Gene Therapy The use of square wave pulses for the transfection of mammalian cells is well established Fakhrai has used a BTX 800 Series in the technique of Electro Immuno Therapy The ECM 830 will enable transfection of mammalian cells while maintaining higher cell viability For many mammalian cells higher transfection efficiencies will also be obtained Mammalian Cell Prote
134. h 1 mm Pin Threaded Collar Continued Glass Catalog No Wire Pellet Connector OD mm BS4 64 1091 1 0 BS4 64 1092 1 2 BS4 64 1093 1 5 BS4 64 0977 i BS4 64 1094 2 0 BS4 64 1363 Hybrid Straight 1 5 ae BS4 64 1365 2 0 BS4 64 1366 Yes 15 BS4 64 1367 i BS4 64 1368 20 Model QRW T10P QRW T12P QRW T15P QRW T17P QRW T20P QSH T15N QSH T17N QSH T20N QSH T15P QSH T17P QSH T20P ea a gt port Compatible with Heka List and Dagan Patch Clamps NOTE Holders without ports are not suitable for patch clamp recording Q SERIES Holders with BNC Connector Glass Q45 BxxP Model OD mm Catalog No Wire Pellet Connector BS4 64 1109 Wire Straight 1 0 QSW BION BS4 64 1110 1 2 QSW B12N BS4 64 1111 15 QSW B15N er BS4 64 0746 17 QSW B17N 6 BS4 64 1112 QSW B20N BS4 64 0825 QSW B10P pr BS4 64 0826 QSW B12P BS4 64 0827 QSW B15P KE T BS4 64 0979 QSW B17P 6 BS4 64 0828 QSW B20P BS4 64 0845 45 Q45W B10N BS4 64 0846 Q45W B12N BS4 64 0847 Q45W B15N BS4 64 1099 Q45W B17N BS4 64 0848 Q45W B20N BS4 64 1059 Yes Q45W B10P BS4 64 1060 Q45W B12P BS4 64 1061 Q45W B15P BS4 64 0969 Q45W B17P BS4 64 1062 Q45W B20P 11 000 Specialty Products to Enhance Your Bioresearch Q Series Holders Q SERIES Holders with BNC Connector Continued Catalog No Wire Pellet Connector Port Glass OD mm Model Electrode Holders BS4 64 0865 BS4 64 0866 BS4 64 0867 BS4 64 11
135. h Series 20 chambers The platform functions as a base for the jacket chamber sandwich and provides the clamping pressure to make a tight seal Mounting plat forms are machined from black Delrin and are compatible with all Series 20 stage adapters Ordering Information Assembly of Series 20 chamber with cooling jacket and stage adapter Aperture Catalog No For Chamber Model Size BS4 64 0354 PHC 1 RC 22 22C 24N 17 0 x 37 mm RC26 26G 26GLP 26Z RC 27 27N 27NE RC 28 RC 27L RC 29 BS4 64 0355 PHC 2 RC 22 22C 24N 15 mm Dia RC 26 26G 26Z 26GLP BS4 64 0356 PHC 3 RC 27 27N 27NE 8 0 x 25 mm U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M113
136. hambers for 25 mm Coverslips The RC 40HP uses 25 mm cov erslips to form the chamber bot tom Tapered 6 5 mm interior sides provide clearance for pipettes injectors etc and allow for a greater bath depth than the 40LP Series The RC 40HP Chamber is sup plied with one package of 1 5 coverslips 25 mm diameter The RC 43C is the closed bath member of the Series 40 family The closed bath promotes an even and continuous solution exchange and eliminates changes in focus due to alterations in bath height Studies involving physiologi cal bicarbonate buffers can be performed with no gas loss at the chamber interface Specialized Tools For Bioresearch Fluid is delivered to a 20 gauge stainless steel perfusion inlet port and removed from the chamber by means of a similar outlet port The top coverslip is held in place with a retainer a thin coating of silicone grease forms the seal The distance between top and bottom coverslips is 1 mm The RC 43C Chamber is supplied with one package each of 1 5 coverslips 25 and 18 mm round respectively and spare O rings Additionally the RC 43C is supplied with 10 feet of PE 160 and PE 90 Polyethylene Tubing M84 BATH DIMENSIONS 25 mm coverslip 18 mm top coverslip Volumes are for 1 mm solution height Catalog No Model BS4 64 0370 RC 40HP BS4 64 0371 RC 43C Accessories Replacement Parts BS4 64 0375 QE 1 BS4 64 0384 CS18R BS4 64 0705 CS25R BS4 64 0714
137. han intramuscular DNA injection alone Mir et al 1999 used various types of electrodes to deliver genes into skele tal muscles of various species rat mice rabbits monkeys resulting in 2 to 4 log fold increase in expression DNA vaccination by direct in vivo administration of plasmid based DNA vectors has proven to be very effective in animal models Electroporation enhances gene expression in muscle greatly making it possible to induce immune response in large animals without using large amounts of DNA Therefore in vivo electroporation is proven to be especially useful at increasing effective ness of DNA vaccinations in large animals Other Applications Electrodes similar to the 2 Needle Array have been used for a variety of applications Salford used an electrode of this style with a 600 Series to deliver bleomycin into rat gliomas doubling animal survival time Yamaguchi used a similar electrode with an 800 Series to deliver bleomycin into subcutaneously grafted mouse bladder carcinoma resulting in complete regressions of the tumorous tissue Nishi used this type of electrode with an 800 Series to deliver a plasmid DNA construct into rat gliomas resulting in expression of beta galactosidase in the area between the electrodes Muramatsu used similar electrodes with an 800 Series in his studies of LIVGET techniques in mouse testis concluding that electroporation is superior to microparticle bombardment To Use Wh
138. highly con ductive lt 10 K Q but not porous There is no solution migrating Specifications Low Leakage Reference Electrodes Diameter 2 mm 0 08 in 5 mm 0 2 in Length 130 mm 5 1 in 130 mm 5 1 in Construction PEEK PEEK Leakage Rate lt 5 x 10 12 l min 5 x 10 12 l min Filling Electrolyte 3 M KCl 3 M KCI Electrical Connection 2 mm pin on 30 in lead 2 mm pin Junction Resistance lt 2 5K OF lt 1K Ot Temperature Range 5 to 80 C 5 to 80 C Storage 3 M KCI 3 M KCI Catalog No BS4 69 0024 BS4 69 0025 Polyetherether Ketone tin 1M potassium chloride Flexible Reference Electrode E Luer Cap Reference Solution Flexible Tubing Plastic Holder 2 in long 0 7 mm OD Ag AgCl Porous Junction Tee Luer Fitting For Ag AgCl Reference Electrodes see pages M30 and M31 through the junction in either direction The reference is totally leak free The junction is resistant to most commonly used organic solvents It offers excellent resistance to acetonitrile DMSO THE MEK acetone dichloromethane esters alcohols and ketones hydrofluoric acid or common dilute acids and bases Unlike conventional reference elec trodes there is no need to store them in concentrated chloride solu tion If the electrode is left dry for a long period of time it should be immersed in water for few hours to recover Two sizes of LEAK FREE reference electrodes are available see ordering chart below for details
139. ic stirring assembly couples with a 2 x 7 mm stir bar in the cup to keep fresh solution moving past the electrode tip Alternatively the chamber can be set up for in line oxygen measure ment by inserting the appropriate flow cell into the chamber block Separate high and low flow cells cover a range of flow rates Catalog No Product BS4 56 4930 Chamber Block with Batch and Flow Cells BS4 61 0137 Chamber Block with Flow Cells Only Note Electrode stirring assembly and stirring controller not included If needed order separately or order part number BS4 61 0131 Instech Oxygen Electrodes Single Chamber Systems with Electrodes For customers that already have an amplifier we offer the following Catalog No Product BS4 61 0131 Batch Flow Chamber System for Instech Amplifiers BS4 61 0132 Batch Flow Chamber System for YSI Amplifiers BS4 61 0134 Standard Electrode with YSI Compatible Cable BS4 61 0133 Batch Flow Chamber System for Diamond General Chemical Microsensor BS4 61 0135 Standard Electrode with Diamond General Compatible Cable Dual Channel Oxygen Amplifier This amplifier generates the potential that operates our standard elec trodes BS4 56 5002 and also converts the nanoamp electrode current into a voltage to measure pO It contains two independent channels each with gain and zero offset controls and analog outputs A 3 5 digit LED dis play can be set with a selector switch to display either channel or the dif
140. ick BS4 64 1305 E201 Pellet 2 0 x 4 mm D x Thick BS4 64 1313 E210 Pellet 2 0 x 2 mm D x Thick BS4 64 1306 E202 Disc 4 0 x 1 mm D x Thick BS4 64 1307 E203 Disc 8 0 x 1 mm D x Thick BS4 64 1308 E204 Disc 12 5 x 1 mm D x Thick Ag AgCl Pellet and Wire Electrodes Pellet electrodes have cylindrical pellet embed ded on 25 x 70 mm D x L wire Wire electrodes have a uniform coating of Ag AgCl over the last 10 mm of a 0 25 x 70 mm D x L wire Maximum diameter of the Ag AgCl matrix is 0 8 mm Note All electrodes suitable for probing tissues Cat No Model Product BS4 64 1309 E205 Pellet Electrode E205 E206 E255 1 0 mm D BS4 64 1310 E206 Pellet Electrode 2 0 mm D BS4 64 1317 E255 Wire Electrode 0 8 mm D Disc Electrodes With Lead Wires These disc type electrodes are made with a flexible 20 cm lead wire attached to the back The connection is encapsulated so that only the surface and edges of the electrode can make contact These elec trodes can be used as direct contact skin electrodes or subcuta neously in temporary applications on research animals Catalog No Model Product BS4 64 1314 E242 Disc Electrode 4 0 mm D BS4 64 1315 E243 Disc Electrode 8 0 mm D BS4 64 1316 E244 Disc Electrode 12 5 mm D Flat Tip Probes Ag AgCl cylinders encapsulated aE207 in sturdy insulating epoxy tubes these probes are ideal for use in ionic solutions They can be resurfaced frequently with fine sandpaper Termi
141. icro Wes wes pipettes and micro electrodes used primarily in intra cellular research Clark Electromedical will con tinue to trade under the Clark brand name however all manufacturing marketing and sales support has been relocated to the Harvard Apparatus Edenbridge facility Borsilicate Capillaries The properties of borosilicate glass make it the most popular materi al among researchers for the fabrication of electrodes and micro pipettes Its low softening temperature combined with its mechanical strength chemical durability high electrical resistivity and its ability to withstand thermal stress make these capillaries the most widely used in the world Clark Borosilicate Capillaries are offered in a variety of diameters and wall thickness standard or thin walled with or without an inner filament They are available in fused multi barrel configurations as well as in Theta style Single barrel glass is available in 75 mm 3 in 100 mm 4 in and 150 mm 6 in lengths Specifications Composition 80 9 SiO 12 9 B O 4 4 Na O 1 8 Al O Softening Temp 815 C Dielectric Constant 4 7 Borosilicate Standard Wall without Filament Borosilicate Thin Wall without Filament o a O Borosilicate Thin Wall without Filament Borosilicate Standard Wall without Filament Catalog No 07D Quantity Catalog No 07D ID Quantity J BS4 30 0037 1 0mm 0 78mm 75mm 500 BS4 30 0018 1 0mm 0 58mm 75mm 500 BS4
142. idation results and has negative effects on the environment The kit includes Materials Pack of 1 5 mm OD glass capillaries with fila ment 10 m pure silver wire 0 2 mm dia fine solder wire emery paper Scotch Brite clean ing sponge KCl and NaCl powder connecting pins for the microelectrodes and sealing wax Tools 18 W 230 V miniature curved soldering tweezers straight soldering tweezers fixation tweezers clamping stand as aid for soldering the micro pins electrolytic chloridation Auto Chloridizer transport and storage container for micro electrodes special microelectrode holder with miniature socket and illustrated book about the microelectrode technology Product Microelectrode Starter Kit 115 VAC 60 Hz Microelectrode Starter Kit 230 VAC 50 Hz Catalog No BS4 73 0008 BS4 73 0009 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 129 M29 Reference Electrodes Reference Cells amp Ag AgCl Electrodes Specialized Tools For Bioresearch M30 Why Silver Silver Chloride Ag AgCI Ag AgCl electrodes have long been recognized for their superiority in sensing bioelectric signals The electrochemical properties of Ag AgCl are such that biopotential waveforms are faithfully reproduced The main feature that makes them superior to other metals is their low offset potential In a biomedical situation the offset potential may be unstab
143. ies and other applications requiring a larger bath size Perfusing the Chambers Perfusion can be a pump driven or gravity fed sys lem Flow rates of 10 ml min or greater are possible but flows of 0 5 to 5 ml min are the norm Standard PE 160 polyethylene tubing or equivalent inserts directly into the input port Solutions are removed by suction with either a vacuum source or pump Open bath chambers are equipped with a stainless steel suc tion tube which is adjustable for control of solution height Multiple perfusion lines can be connected to the chambers by using one of the MP or MPP Series manifolds see page M140 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASojolg 129 M99 Special Chambers Systems Series 20 Closed Bath Imaging Chambers Avi RC 20 in P 5 RC 21B with RC 21BRFS with Platform P 2 Platform P 2 Platform New Improved Suction Tube Holder Small Volume RC 20 RC 20H A Imaging Chambers RC 20 48 pl amp RC 20H 60 pl RC 20 and RC 20H are very small volume imaging chambers featur ing fast perfusion exchange and short working distance Two 15 mm diameter coverslips enclose the bath Distance between top and bottom cover slips is 1 mm The top coverslip is held in place with a retainer The sample coverslip is placed on the chamber bottom and the assembled chamber is placed in the chamber platform Two bath sizes are available The smaller
144. iffusion Chambers See pages M166 to M170 for complete details on those products and many others M73 Microinjection amp Microperfusion Specialized Tools For Bioresearch M74 ca ZonaDrill The New ZonaDrill is a class 1 Laser System that incorporates the latest in fiber optic technolo ey to provide the most compact laser system currently available The ZonaDrill laser system is mounted via the epifluorescence port or side port of all popular brands of inverted microscope It can also be used in conjunc tion with all brands of micromanipulators and microscopes Rapid Zona removal is possible using the non contact laser Laser ablation is much faster than mechanical methods such as piezo electric devices and manual micromanipulation Improve your effi ciency and effectiveness in the lab with this market leading product A visible pilot laser is used to target the main invisible ablation laser and the digital display informs the user when the laser is primed and ready to fire The pulse duration of the laser is adjusted according to the hole size required The new digital display and compact hand control are unique features of this Laser system allowing easy and quick operation of the laser The latest ZonaDrill laser system now incorporates a new laser objective which provides a very high quality high resolution image It has also been designed for high transmission of the laser so that shorter puls
145. ignificantly increasing the viability of the tissue culture Whether you have an inverted or upright microscope it is possible to achieve media heating and or cooling under static or dynamic conditions We also have Micro Incubators that work in either an open or gas tight environment Four different Micro Incubators and three different companion chambers are described on the following pages including our new CSMI micro incubator for rectangular slides In addition we offer a fifth micro incubator system our model PSMI for customers in need of the shallow depths and low angles of attack typically required for patch slice studies The TC 202A temperature controller see page M125 is avail able which is perfectly matched to the heating and cooling requirements of all the Micro Incubator systems Specifications Temperature Range 5 to 10 C below ambient to 50 C without water cooling Disposable Chambers Accommodated 1 Nalge Nunc chambered slides amp cover slips 2 Becton Dickinson Falcon chambered slides Microscopes 1 Zeiss Axiovert with attachable mechanical stage Accommodated 2 Leica DAS Microscope DMIL and DMIRB E with attached mechanical stage 3 Nikon Diaphot 4 Olympus IX50 70 or IMT 2 fixed stage Peltier TED Current Rating 6 ADC max 100 kQ at 25 C YSI 44011 26 AWG Teflon capillary up to 4 lines can be installed simultaneously Built In Plate Thermistor Perfusion Inlet Lines Perfusion Rate 3 ml min n
146. ile grasping the Needle Array handle simply position the handle over a needle array assembly and push to secure the needle array onto the handle Attach the handle to a BIX pulse generator via the high voltage banana cables Remove the needle safety shield place into the tissue and deliver the pulse Discard the needle array and prepare for the next experiment Specifications Voltage Range 0 to 500 V 1 usec to 99 msec in PBS Pulse Length Range Electrode Handles Model 530 Handle Length 8 cm 3 2 in Handle Material Delrin Electrode Diameter 10 mm Model 532 Handle Length 8 cm 3 2 in Handle Material Delrin Electrode Diameter 5 mm Two Needle Array Assemblies Model 531 Needle Length 20 mm Needle Material Stainless Steel Needle Diameter 10mm Model 533 Needle Length 20 mm Needle Material Stainless Steel Needle Diameter 5mm Generator Compatibility ECM 830 630 and 399 Catalog No Model Product BS4 45 0205 530 2 Needle Array Handle 10 mm requires BS4 45 0120 BS4 45 0120 531 2 Needle Array 10 mm pkg of 6 requires BS4 45 0205 BS4 45 0206 532 2 Needle Array Handle 5 mm requires BS4 45 0121 BS4 45 0121 533 2 Needle Array 5 mm pkg of 6 requires BS4 45 0206 BS4 45 0167 530 KIT Includes 2 Needle Array 10 mm BS4 45 0120 and Handle 10 mm BS4 45 0205 BS4 45 0168 532 KIT 2 Needle Array 5 mm BS4 45 0121 and Handle 5 mm BS4 45 0206 References 1 Aihara and Miyazaki Nature Biotechnology 16
147. illary through which solutions may be added during the course of a respiration run Stainless steel magnet ic spinbars are supplied together with a special 1 pl syringe supplied with MT200 and a 5 pl syringe with the MT200A for solution addi tions An alternative version with a large volume 0 3 0 5 and 1 0 ml and a PTFE coated stirbar is also available Catalog No Model Product BS4 69 3033 MT200 Mitocell Miniature Respirometer 50 100 ul BS4 72 6186 MT200A Mitocell Miniature Respirometer 0 3 0 5 1 0 ml BS4 72 6187 SK205 Replacement Glass Plunger for MT200 BS4 72 6188 SK206 Replacement Glass Plunger for MT200A 11 000 Specialty Products to Enhance Your Bioresearch 6 Electrode Respirometer Model RC 650 E For use with the 928 6 channel oxygen e Ability to carry out replicate experiments utilizing the advanced recording and analysis features of the Strath or kelvin 928 system system for replicate respiration measurements of cell suspensions microbial suspensions mitochondria enzyme preparations The respirometer consists of a sealed water both containing six respirome ter cells located above a six position magnetic stirrer Electrode holders with a hole in their tips through which the membrane of the 1302 electrode protrudes make a sliding fit into the cells As the holder is inserted into the preparation the sloping face at its tip gathers the air into the slot which runs the complete length of the holder
148. ime 10 sec Slope 55 mV per pH range of 1 to 14 Impedance 200 M Q Stability 0 05 pH per day Dimensions L x D 91 4 x 1 6 mm 3 x 0 06 in Product Combination pH Electrode for Free Flap Surgery Catalog No BS4 69 0496 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 29 M25 pH and Reference Electrodes Specialized Tools For Bioresearch M26 Protein Resistant pH Electrodes Minitrode P Measuring the pH of biological samples containing proteins is diffi cult Proteins in solution can com bine with silver ions from the elec trolyte to clog the pH electrode diaphragm This leads to long response times inaccurate pH measurements and eventually dis carded electrodes These protein resistant electrodes are designed for rapid accurate and repro ducible pH measurements of pro tein containing solutions Unique to these pH electrodes is the specially formulated Protelyte reference electrolyte Protelyte electrolyte prevents the precipitation of proteins on the electrode diaphragm You get faster more accurate reproducible pH measurements of your samples Select from five specialized electrodes The best electrode for you depends upon your applications physical size limitations and temper ature range Each has a pH range of 2 to 11 Specifications Outer Catalog No Electrode Diameter Temp Range BS4 69 0070 Minitrode P 100
149. in Drug Electroincorporation Square wave pulses have been used to electroincorporate a variety of molecules into mammalian cells Tsong has used square wave to load drugs into erythrocytes4 while Marrero has used a BTX 800 Series and BIX Model 366 Petri Dish Electrode to incorporate biologically active antibodies into RASM cells 11 000 Specialty Products to Enhance Your Bioresearch Electroporation Products ECM 830 Square Wave Electroporation System In Vivo Applications Recent advances by BTX have allowed researchers to perform in vivo electroporation In vivo Electro Gene Therapy has significant advantages over viral mediated gene transfer biolistics and the use of cationic Lipids Nuclear Transfer BIX square wave systems have been used by the experts in mammalian embryo manipulation techniques in nuclear transfer for applications ranging from pharmaceutical production to organ generation Plant Applications Intact plant tissue and protoplasts may be transformed using electropo ration and references indicate that square wave is indeed superior to exponential decay for plant protoplast transformation Bacterial and Yeast Electroporation The ECM 830 is capable of reproducing 800 Series bacterial protocols including all four coli electrotransformation protocols referencing transformation efficiencies equivalent or superior to exponential decay results Square wave pulses are used to transform yea
150. in various sam ple types including viscous samples Since this technology is immune to interference caused by pH or salinity change or from changes in ionic strength they can be used in a wide range of experiments The sensors are designed to have a high resistance to bio foul ing Temperature compensation via an exter nal temperature probe provides added accu racy and stability to the measurement of oxy gen is samples were temperature may vary The FO ton Fiber Optic Oxygen Sensing System uses the fluorescence of an organic ruthenium based dye to measure the absolute concentration of oxygen Optical fiber carries excitation light produced by an integrated blue LED light source to the thin film rutheni um dye coating at the fiber optic probe tip Fluorescence generated at the tip is collected by the probe and carried back by the fiber to a high sensitivity spectrometer When oxygen in the gas or liquid sample diffuses into the thin film ruthenium coating the fluorescence is quenched The degree of quenching corre lates to the concentration of oxygen Ruthenium tipped sensors are available in dif ferent sizes and styles of fiber optic sensors to meet a number of application The FO ton Fiber Optic Oxygen Sensing System consists of a high sensitivity miniature fiber optic spectrophotometer light source USB interface to connect the system to an IBM compatible benchtop or portable computer and a selection of various size pro
151. ine com M57 Electroporation Products CD ECM 630 Exponential Decay Wave Electroporation System _ Specialized Tools For Bioresearch M58 CE Precision pulse provides more reliable and reproducible results Over 8300 RC time constants in low voltage mode allows unparalleled performance for precise optimization of plant and mammalian protocols 126 RC time constants in high voltage mode allows unparal leled performance for precise optimization of bacteria and yeast protocols Low voltage mode 1 V discrimination allows for greater repro ducibility for low voltage applications including mammalian cell transfections 6000 A limit low voltage mode 3000 A limit high voltage mode allows for unparalleled power to drive exclusive BTX specialty electrodes Monitoring of peak voltage and time constant for optimization of protocols troubleshooting and documentation of parameters Generator will not be damaged in the event of a short circuit External safety stand protects generator from damage caused by spills All digital user interface design allows for improved operation Single dial control simplifies parameter settings Voltage range of 10 to 2500 V is the basis for a generator capa ble of electroporating all cell types Unparalleled resistance range of 25 to 1575 Q in 25 Q incre ments and discrimination in both LV and HV Resistance setting on None in LV mode reproduce protocols from competitive electropor
152. ion of the alignment and fusion process The Microslide 450 is composed of a glass microslide and two strips of 0 5 mm stainless steel tubing which functions as electrodes to provide a diver gent field These wires are set apart at different gaps to provide various field strengths and volumes The Micrograbber is used to connect the Microslide 450 to the Coaxial Connection Cable which will then be hooked up to the ECM 2001 generator Microslide 453 has two stainless steel square bars that are set apart at various gaps to provide homogeneous fields The Square Post Cable is used to connect the Microslide to the Coaxial Connection Cable which will be hooked up to the ECM 2001 generator Applications The Microslides have been used for embryo manipula tion and cell or protoplast fusion Advantage of the Microslide is that the process of dimmer formation can be observed under the microscope Catalog No Model Product BS4 45 0103 450 Microslide 20 ul pkg of 10 0 5 mm gap BS4 45 0104 450 1 Microslide 40 ul pkg of 10 1 0 mm gap BS4 45 0105 453 Microslide 3 2 mm gap pkg of 1 BS4 45 0106 453 10 Microslide 10 mm gap pkg of 1 BS4 45 0210 5343 Coaxial Banana Plug Connection Cable 10 ft BS4 45 0200 464 Micrograbber Cables for use with Microslide BS4 45 0103 and BS4 45 0104 BS4 45 0201 465 Square Post Cables for use with Microslide BS4 45 0105 and BS4 45 0106 References 1 BTX Protocol PRO117 Personal communicati
153. isit our website at www harvardappa ratus com 11 000 Specialty Products to Enhance Your Bioresearch pH and Reference Electrodes Microminiature pH Electrodes Combination pH Electrode in 18 Gauge SS Tube Luer Cap Reference Electrolyte l Reference ee SS Tube 3 in long 1 25 mm OD Coax Cable BNC 4 ft pH bulb Plastic Holder This miniature combination pH reference electrode is mounted in an 18 gauge 3 inch x 1 25 mm OD stainless steel tube The stainless steel tube provides protection to damage of the internal miniature glass pH sensor It does not require an external reference electrode The refer ence solution can be replaced The electrode is stored wet Tip length is 1 5 mm Response Time 10 sec Slope 55 mV per pH range of 1 to 14 Impedance 200 M Q Stability 0 05 pH per day Dimensions L x D 76 x 1 25 mm 3 x 0 005 in Catalog No Product BS4 69 0492 Combination pH Electrode in 18 ga SS Tube Model IC 201 Combination pH Electrode in 11 Gauge SS Tube Luer Cap Reference Electrolyte Reference Electrode Coax Cable BNC 3 5 ft f Plastic Holder 1 25 in long 3 mm OD pH bulb This miniature combination pH reference electrode is mounted in an 11 gauge 1 25 inch x 3 mm OD stainless steel tube The stainless steel tube provides protection to damage of the internal miniature glass pH sensor It does not require an external reference electrode The refer ence solution can be
154. it Accessories Replacement Parts BS4 64 0351 AR 4 Replacement Adapter Ring Kit for 35 mm Dishes BS4 64 0100 TC 324B Single Channel Heater Controller BS4 64 0101 TC 344B Dual Channel Heater Controller BS4 64 0106 CC 28 Cable Assembly for Heater Platforms BS4 64 0102 SH 27B Solution Heater BS4 64 0357 MCK 1 Magnetic Clamp Kit M86 11 000 Specialty Products to Enhance Your Bioresearch Chambers for 35mm Cell Culture Dishes PDMI 2 Open Perfusion Micro Incubator PDMI 2 Micro Incubator Typical Setup The PDMI 2 operates equally well under static or perfused condi tions When operated with perfu sion the micro incubator s unique design places flexible tubes carrying inflowing perfusate in contact with a temperature reg ulated Peltier heat pump driven plate thus heating or cooling the incoming fluid to a command temperature This eliminates the need for auxiliary preheating or cooling The Peltier plate heats or cools the cell carrying central chamber uniformly when no perfusate is flowing Heats and cools 6 to 50 C e Maintains required cell environment for hours gt Piirand O gt temperate Peltier heat pump Flexible chamber option Easily change perfusion fluid Makes temperature cycle experiments easy Flexible Temperature Range The operating temperature range of the PDMI 2 Open Perfusion Micro Incubator is nominally 5 to 50 C when used with the TC 202A Temperature Controller see
155. ith medium so as to be free from air bubbles the venting cannula is used to remove air bubbles from the system The pO electrode measures the fall in chamber pO due to the oxygen consumed by the cells Since pO measurement is seriously affected by temperature the chamber is provided with opti mal thermostating Operation of the Oxystat The HSE HA Oxystat can operate like conventional setups as a closed system The oxygen consumption is calculated from the slope of the recorded pO reduction Since the chamber is very well screened against oxygen diffusion it is possible to measure pO down to 0 1 mmHg The really new application of the Oxystat is however an open system with controlled addition of oxygen through the solution so that O consumption can be measured at constant pO pO Measurement Ea Compares Measurement with Setpoint Recorder Continuous Recording of pO VO and Fluorescence Figure 2 Oxystat System The operation of the Oxystat as an open system is indicated in Fig 2 The pO signal is examined by the controller if it differs from the preset value a pump is operated to introduce oxygen rich solution into the chamber This raises the pO in the chamber back to the set value and the pump is then stopped again As a result a flow equilibrium is estab lished through which despite the oxygen consumption of the incubated cells the chamber pO is maintained close to the set value 5 The
156. lastic Holder pH Electrode 0 3 mm Coax Cable BNC 4 ft oax Cable ey 25 ga Needle 5 8 in L 0 5 mm OD This is a microminiature pH electrode mounted in the lumen of a fine hypodermic needle This configuration allows for easy penetration and insertion in all types of tissue for on line real time monitoring of pH This pH electrode does not contain an external reference To use this electrode and external reference electrode such as the BS4 69 0490 or similar reference electrode must be used The electrode is stored dry Specifications Response Time 15 sec Slope 55 mV per pH range of 1 to 14 Impedance 1500 M Q Stability 0 05 pH per day Product Single pH Electrode in 25 ga Needle Catalog No BS4 69 0491 BS4 69 0490 Flexible Reference Electrode see page M28 Combination pH Electrode in 20 Gauge Needle Luer Cap Reference Electrolyte Plastic Holder Reference Electrode Coax Cable BNC 4 ft 20 ga Needle 1 in pH Electrode 0 5 mm This is a miniature combination pH reference electrode that is mount ed in a 20 gauge 1 inch x 0 5 mm OD stainless steel tube The stain less steel tube provides protection to damage of the internal miniature glass pH sensor It does not require an external reference electrode The reference solution can be replaced The electrode is stored wet Tip length is 1 8 mm Response Time 10 sec Slope 55 mV per pH range of 1 to 14 Impedance 200 M Q Stability 0 05 pH per day
157. le or unpredictable Long term changes in offset potential appear as baseline drift and short term changes as noise on the trace Thus it is desirable that this potential be as low as possible The electrode that shows minimum offset is the Ag AgCl electrode As the silver chloride is ionized chloride ions from the electrode are exchanged for similar ions in the tissue or electrolyte The silver chloride is rela tively stable regardless of the direction of the polarizing current flow To a great extent the performance of Ag AgCl electrodes depends on their formulation Our electrodes are made from very high purity materials A careful proprietary process results in a fine grain homogeneous mixture of silver and silver chloride A careful sintering process strengthens and stabilizes the entire electrode assembly Typical values of the key parameters measured in 0 9 saline between pairs of electrodes the same size are 1 DC Offset Voltage 180 pVolis 2 Drift 25 pVolts hour 3 Noise 1 pVolt peak to peak 0 1 1000 Hz with 50 60 Hz eliminated Ag AgCl Pellet and Disc Electrodes These six electrodes are ideally suited for making custom assemblies of skin electrodes and other bioelectric recording and stimulation apparatus All have a pure silver wire 25 mm diameter x 7 mm embedded in the Ag AgCl matrix This wire can be easily soldered or twisted to other wires Catalog No Model Product BS4 64 1304 E200 Pellet 1 5 x 3 mm D x Th
158. le to N I S T pr eee aye ve ave a Solution Color Red Green Blue Yellow Yellow 1 Bottle 500 ml BS4 69 0083 BS4 69 0084 BS4 69 0085 BS4 69 0086 BS4 72 7140 Multipack 500 ml x 3 BS4 69 0088 BS4 69 0089 BS4 69 0090 BS4 69 0091 oO a a 2 5 Catalog No Product ps4 69 0092 Buffer Set One 500 ml Bottle of 4 01 7 00 and 9 21 J BS4 72 7141 Buffer Set One 500 ml Bottle of 4 01 7 00 and 10 01 yea 72 Figure 1 mi H ih D t aha bs E m kad ri Step 1 Unscrew cap to access Step 2 Squeeze CALPAK bottle to Step 3 Insert electrode for calibra Step 4 Turn CALPAK bottle on its calibration compartment force buffer into compartment tion side to dispose of used buffer Figure 2 Calibrating Electrodes in the CALPAK Dosing Bottle U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M27 Reference Electrodes Reference Electrodes m Specialized Tools For Bioresearch M28 B34 69 0025 eT BS4 69 0023 LEAK FREE Reference Electrodes The LEAK FREE reference electrodes were developed to eliminate the problems associated with the use of conventional porous junction based reference electrodes such as clogging sample contamination electrolyte loss changing of ionic strength of the sample and the difficulties with organic based solvents They utilize a unique junction that is
159. lse generator via a Model 5343 Banana Coaxial B84 45 0122 542 Cable The Genepaddles may be positioned in parallel at a Genetrode Holder Inter Electrode Gaps 1 to 10 mm in 1 mm increments All Genepaddle electrodes are gold plated noninvasive electrodes Genepaddles 3 x 5 mm pra BS4 45 0123 543 Genepaddles 3 x 7 mm predetermined gap within a tissue using the Model 515 Genetrode BSAA U a ere Holder Genepaddles may be cleaned with a mild detergent and sterilized with ethanol or ethylene oxide Properly maintained 59445 0201 a99 i o al Genepaddles have a life span of approximately 200 sets of pulses BS4 45 0210 5343 Banana Coaxial Connection Cable and are compatible with most BTX Electroporation Systems BS4 45 0169 542 KIT Includes Genepaddle 3 x 5 mm joe BS4 45 0122 Holder Applications BS4 45 0203 and Cables In Vitro Embryo Gene Delivery BS4 45 0201 and BS4 45 0210 Genepaddles were designed for In Vitro Embryo Gene Delivery BS4 45 0170 543 KIT Includes Genepaddle 3 x 7 mm IVEGD in mouse embryo The electrodes are placed anterior and BS4 45 0123 Holder posterior to the embryo following injection of the molecule of inter BS4 45 0203 and Cables est and an electroporation pulse is delivered using a BTX PON belie a ull Note All Genepaddle models require Genetrode Holder BS4 45 0203 and Cables Electroporation System such as the ECM 830 2001 and 630 B54 45 0201 and BS4 45 0210 The electric field introduced by the G
160. luminosilicate provides increased hardness improved chemical durability reduced electrical conductivity and a lower coefficient of thermal expansion Also while the original ratio of a borosilicate capillary inner to outer diameter will remain unchanged over its total taper length alumi nosilicale glass demonstrates a marked tendency to thin out as it is drawn to a tip This bebavior allows extremely fine tips to be formed For more information see 1 Na H Exchange Vaughan Jones RD Grinstein Press Ch 1 p 8 2 Effects of intracellular and extracellular pH on contraction in isolated mammalian cardiac muscle Bountra C amp Vaughan Jones R D Journal of Physiology Volume 418 1989 Composition 51 9 SiO 22 0 Al O 7 8 P O0 7 7 MgO 6 9 CaO 2 1 B O 1 4 BaO and 0 2 AsO Softening Temperature 950 C Dielectric Constant 6 2 Aluminosilicate Capillaries with Filament Aluminosilicate Capillaries with Filament Catalog No 0 9 Quantity BS4 30 0110 10mm 0 53mm 75mm 500 BS4 30 0108 10mm 053mm 100mm 500 BS4 30 0109 1 0mm 053mm 150mm 500 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 29 M49 Pipettes Pipette Sterilization and Storage Container This container is a convenient and easy to use pipette storage container It is ideal for both stor age and dry heat sterilization of micropipettes In most applica tion pipettes m
161. lve Lubricant amp Sealant retains viscosity at elevated temperatures better than others we tested The 111 sealant is particularly recommend for heated perfusion systems Product Silicone Lubricant Catalog No BS4 64 0275 11 000 Specialty Products to Enhance Your Bioresearch Series 20 Chamber Platform and j e View thru single coverslip e Laminar perfusion flow e Small volumes fast exchange e Use with most microscopes e Open and closed bath types Special Chambers Systems Series 20 Perfusion Chambers for Imaging and Recording o Clamp Screw Adapter S Side Clamp Heating Element Perfusion Flow Assembly of Chamber The chamber is prepared by applying a thin bead of vac uum grease to the chamber bottom The sample cover slip sample side up is then attached to the chamber This assembly is then placed in the platform and clamped to provide a seal between coverslip and chamber Mounting on the micro scope is done with the appropriate stage adapter The installation is complet ed by connecting the perfu sion lines and any heater cabling required Assembly of the closed type chambers includes installa tion of the top coverslip which precedes the steps outlined above Modular Design Series 20 chambers are a Jamily of models designed to fill a large number of imaging and recording needs The modular design consists of two parts a polycarbon
162. m Thin Wall Borosilicate Catalog No BS4 64 0777 BS4 64 0778 BS4 64 0779 BS4 64 0780 BS4 64 0781 BS4 64 0782 BS4 64 0783 BS4 64 0784 BS4 64 0785 Model G100T 3 G100T 4 G100T 6 G120T 3 G120T 4 G120T 6 G150T 3 G150T 4 G150T 6 OD mm ID mm Length 1 0 10 1 0 l2 12 ile 15 15 1 5 Premium Standard Wall Borosilicate with Filament Premium Standard Wall Borosilicate 0 78 0 78 0 78 0 94 0 94 0 94 1 17 Wee 1 17 A small diameter filament is fused to the glass inside diameter to Catalog No Model OD mm ID mm facilitate rapid solution filling through capillary attraction This is BS4 64 0786 G100F 3 1 0 0 58 75mm 500 especially important for the very small diameter of sharp electrodes BS4 64 0787 G100F 4 1 0 0 58 100mm 500 typically used for intracellular studies and microiontophoresis BS4 64 0788 G100F 6 1 0 0 58 150mm 500 Premium Corning Type 7740 Pyrex BS4 64 0789 G120F 3 1 2 0 69 75mm 350 Corning 7740 Borosilicate is the a BS4 64 0790 G120F 4 1 2 0 69 100 mm 350 most commonly used glass in BS4 64 0791 G120F 6 1 2 0 69 150mm 350 electrode fabrication because of Composition St BS4 64 0792 G150F 3 1 5 0 86 75mm 225 its mechanical strength chemical 4 Na20 _ BS4 64 0793 G150F 4 1 5 0 86 100mm 225 durability electrical resistivity 2 Al203 BS4 64 0794 G150F 6 1 5 0 86 150mm 225 and its ability to withstand ther Softening Temp 821 C BS4 64 0795
163. m x 40 mm coverslip forms the chamber bottom e 54 ul bath volume for 1 mm solution height e Suction tube clamp Order with P 1 or PH 1 Platform Standard accessories supplied are 22 x 40 mm 1 coverslips and PE 160 Tubing 10 ft Catalog No Model Product BS4 64 0381 RC 24N Fast Exchange Recording Chamber Accessories Replacement Parts BS4 64 0707 CS 22 40 Coverslips 22 x 40 mm 1 Box of 50 RC 25 RC 25F TOP VIEW Round Coverslip Suction Reservoir fits on bottom Perfusion Inlet Port Suction Agar Bridge Well Tube Slot SIDE VIEW Lal 133 pl mm RC 25F NOT Sor 90 pl mm RC 25 gt 25 mm BATH DIMENSIONS Volumes are for 1 mm solution Round Coverslip Chambers RC 25 90 pl amp RC 25F 133 pl The bottom of these chambers is formed from standard size round coverslips a widely used culture support for cells destined for phys iological recording and or imaging RC 25 works with either 12 or 13 mm diameter coverslips and RC 25F uses 15 mm coverslips RC 25 is ordered with P 3 or PH 3 platform RC 25F is ordered with P 4 or PH 4 platform see page M112 for platform information These chambers are not designed for coverslip insertion from the top Catalog No Model Product BS4 64 0232 RC 25 Round Coverslip Recording Chamber 90 pl BS4 64 0233 RC 25F Round Coverslip Recording Chamber 133 pl 11 000 Specialty Products to Enhance Your Bioresearch Special Chambers Systems S
164. matic or manually controlled Number of Sequence Repeats 1to9 Dimensions H x W x D 27 9 x 43 2 x 39 4 cm 11 x 17 x 15 5 in Warranty 2 years Power 100 115 VAC single phase 230 VAC single phase Fuse 7A slow blow Weight 47 lbs Catalog No Model Product A BS4 45 0053 ECM2001 ECM 2001 Cell Fusion Embryo q Manipulator Generator Only BS4 45 0003 2001EP ECM 2001 Electroporation System W Includes 2001 Generator BS4 45 0053 O Cuvettes 1 mm 2 mm 4 mm pack of 30 10 each Cuvette Safety Stand BS4 45 0207 and Cuvette Rack BS4 45 0208 ga BS4 45 0005 2001EM ECM 2001 Embryo Manipulation System lt Includes 2001 Generator BS4 45 0053 Microslides BS4 45 0103 BS4 45 0104 BS4 45 0105 Micrograbber Cables BS4 45 0200 BS4 45 0201 and Coaxial Connection Cable BS4 45 02103 BS4 45 0007 2001EF ECM Electro Cell Fusion System Includes 2001 Generator BS4 45 0053 Microslides BS4 45 0103 BS4 45 0104 BS4 45 0105 Micrograbber Cables BS4 45 0200 BS4 45 0201 Meander Fusion Chamber BS4 45 0107 Flat Electrode Divergent Field BS4 45 0108 Electrode Adapter BS4 45 0202 Coaxial Connection Cable BS4 45 02103 Electroporation chambers Cuvettes 1 mm 2 mm 4 mm pack of 30 10 each Cuvette Safety Stand BS4 45 0207 and Cuvette Rack BS4 45 0208 M61 Electroporation Electrodes CD Genetrodes Specifications 0 to 200 V 1 u sec 99 msec in PBS Voltage Range Pulse Length Range In Viv
165. ment Catalog No Model Product C015 Cabe 2 BS4 64 0106 CC 28 Cable Assemblies with Connector for TC 324B 344B Assembly E SN BS4 64 0303 CC 15 Cable Assemblies with Tinned Leads at Connector End ar A in m BS4 64 0304 PU 28 Platform Upgrade Kit with CC 28 Cable Assembly BS4 64 0305 PU 15 Platform Upgrade Kit with CC 15 Cable Assembly M112 11 000 Specialty Products to Enhance Your Bioresearch Series 20 Chamber Platforms amp Stage Adaptors Model PHC 3 shown with mounting platform and mounted in a Nikon stage adapter The Warner PHC Heater Cooler Jackets are designed to bring heating and cooling to our clas sic Series 20 Imaging and Recording Chambers Accurate temperature control from 5 to 50 C can be achieved using the PHC jackets in concert with the oe SC 20 Dual In line Solution Clamps a Heater Cooler Heated or chilled water flows from the SC 20 into Jacket Inlet tube the PHC jacket in direct thermal contact with the chamber bot tom coverslip mR The PHC 1 is used for upright microscopes and provides a complete a Adapter thermal barrier between the chamber forming coverslip and the local environment The PHC 2 and PHC 3 are designed for inverted microscopes and provide either rectangular or round openings See ordering information for chamber jacket compatibility ASojo1g 129 Heater Cooler Jackets are provided with a mounting platform which replaces the standard platforms used wit
166. ment of gold plated 1 mm plugs and jacks with or without wires Useful for grounding shielding etc Catalog No BS4 64 1325 BS4 64 1326 BS4 64 1322 BS4 64 1323 BS4 64 1324 BS4 64 1328 BS4 64 1327 Model WC1 10 WC2 5 PJ1 10 PJ2 5 TJ1 3 WA30 5 WA10 5 Product 1 mm Pin pkg of 10 2 mm Pin pkg of 5 1 mm Jack Uninsulated pkg of 10 2 mm Jack Uninsulated pkg of 5 1 mm Jack Teflon Insulated pkg of 3 1 mm Pin with 30 cm L 26 ga Insulated Wire pkg of 3 1 mm Pin with 10 cm L 25 mm D Bare Silver Wire pkg of 2 ASoj01g 129 M31 Electrode Holders E Series Holders M32 ERW FxxN E45 MxxP Lo Applications e Intracellular Recording E45 FxxN ba e Microiontophoresis Body Material Acrylic Body Styles Straight 45 and 90 Connectors 2 mm Pin or 2 mm Jack Coupling Ag Wire or Ag AgCI Pellet Port 2 mm diameter Polycarbonate Vent 0 8 mm diameter Hole Standard Glass Sizes 1 0 1 2 1 5 and 2 0 mm ERP MxxP E SERIES Straight Body Holders Catalog No BS4 64 0992 BS4 64 0993 BS4 64 0994 BS4 64 1260 BS4 64 0995 BS4 64 0996 BS4 64 0997 BS4 64 0998 BS4 64 0980 BS4 64 0981 BS4 64 0982 BS4 64 0983 BS4 64 0984 BS4 64 0985 BS4 64 0986 BS4 64 0987 BS4 64 1011 BS4 64 1012 BS4 64 1013 BS4 64 1014 BS4 64 1015 BS4 64 1016 BS4 64 1017 BS4 64 1018 Glass OD mm Model ESP M10N ESP M12N ESP M15N ESP M20N ESP M10P ESP M12P E
167. mers wax inducing instrument capillary glass tubing hammering rod 3 sticks sealing cement 4 80 mm microtool holders 15 watt bulb weight set 0 5 to 17 gm in 1 gm incre ments 31 ga heating element wire 1 pkg 2 x 150 mm pyrex glass rod forceps and 2 angu lar microtool holders U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M53 Pipette Pullers De Fonbrune Microforge Fabrication Examples A Cupping glass Bent at an angle for positioning in a moist chamber F Various types of micro needles with plain and pediculated points Hook and needle with eye K Spatula loop Light coming through the opening is darkened by a small film of transparent lacquer Specifications Tool Movement Vertical Horizontal m Specialized Tools For Bioresearch Electrode Movement B Three types of cupping glasses a rounded tip b rounded tip slightly constricted c blown cupping glass G Retaining hook L Micro gauge The horizontal portion of this gauge is made with a hollow cylinder 12 7 mm 0 5 in 10 1 mm 0 4 in C Orifices of capillary tubes a Sharp edge b Smooth edge c Constricted orifice H Different types of micro pipettes a b c terminal orifice d and e lateral orifice M Probe a and cannula b BS4 69 0384 BS4 69 0438 Catalog No D Local constrictions of a capillary tube A circular
168. ml min 17 x 152 mm 0 67 x 6 0 in overall 0 5 kg 17 9 oz Dimensions H x Dia Weight Product PSMI Patch Slice Micro Incubator with PS CSD Copper Cooling Tube for PSMI Catalog No BS4 65 0044 BS4 65 0109 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com e Ambient to 50 C The LU CB1 Leiden Micro Incubator provides stable culture conditions temperature pH aseptic isolation and osmolarity during continuous or intermit tent observations when media temperatures between ambient and 50 C are desired e Designed by top teachers at Leiden University e Proven design in use for nearly two decades e Open design for easy cell access The LU CBI is used primarily for static no perfusion chamber media applications It features excellent mechanical and optical access to cells or tissue Measuring probes temperature pH etc recording electrodes and micropipettes can easily be manipulated in and out of the chamber Aseptic isolation of the culture media can be provided by using a layer of non toxic mineral oil A gas flow is directed over the oil to control the micro environment including pH and to minimize temperature gradients across the chamber It is possible to use the LU CB1 as a per fused system by preheating the perfusate close to the desired temper ature before it enters the chamber ASojolg 129 Specifications Chamber Options Coming 35 mm Petri
169. mm 12 5 mm RC 20 RC 20H Volumes for 1 mm solution height M100 11 000 Specialty Products to Enhance Your Bioresearch Top Coverslip Retainer Special Chambers Systems Series 20 Closed Bath Imaging Chambers Large Bath Imaging Chambers RC 21B amp RC 21BR TOP VIEW Sealant Groove Secondary Inlet Port RC 21B Both Sides or Air Trap Release Perfusion Inlet Port Suction Reservoir Back Pressure Tubing connects between these two points SIDE VIEW BATH DIMENSIONS RC 21B amp RC 21BR ba 17mm Volume 360 pl Volumes are for 2 5 mm solution height Large Bath Imaging Chambers RC 21B amp RC 21BR 360 pl 2 5 mm These chambers provide a large viewing area and good access for microscope optics at chamber top and bottom The chambers are identical except for the coverslips used 22 mm square for RC 21B and 25 mm round for RC 21BR Distance between top and bottom coverslip is 2 5 mm Bath volume is 360 pl for 2 5 mm of solution An additional port at the bath input may be used to inject substances or for removing bubbles At the bath output a length of tubing is used as a back pressure control to insure a smooth perfusion flow Order with P 2 or PH 2 platform see page M112 Catalog No Model Product BS4 64 0224 RC 21B Large Bath Imaging Chamber BS4 64 0225 RC 21BR Large Bath Imaging Chamber RC 21BRFS 1 mm PIN TOP VIEW Secondary Inlet Port Perfusion Inlet Port 1mm PIN Suction Well SIDE
170. mm 12 mm BS4 64 0758 D3522P 35x 9 9 mm 22 mm BS4 64 0759 D5030P 50x 7 2 mm 30 mm BS4 64 0760 D5040P 50x7 2mm 40 mm Blister Pack Dishes Individual Packaged Sterile Dishes Case of 120 BS4 64 0761 D3512B 35x 9 9mm 12 mm BS4 64 0762 D3522B 35x9 9mm 22mm BS4 64 0763 D5030B 50x 7 2mm 30 mm BS4 64 0764 D5040B 50x 7 2mm 40 mm Note 35 mm Dishes supplied with Triple Vented Lids for optimum ventilation 50 mm Dishes have low side walls to facilitate microinjection 11 000 Specialty Products to Enhance Your Bioresearch Chambers for 35mm Cell Culture Dishes Micro Incubators Accessories Catalog No Model Product Zeiss Fixing Miscellaneous metiorm BS4 65 0047 LU ASP Leiden Aspirator Cover Slip Dish Fluid Level Setting Device 38 mm H BS4 65 0052 MI M Mini Magnets for Top Surface of Micro Incubators LU CB 1 PDMI 2 or PSMI set of 4 BS4 65 0053 PS CSD Patch Slice Cover Slip Dish for PSMI BS4 65 0054 LU PD Sleeve of 35 mm Petri Dishes pkg of 20 BS4 65 0055 BSC TH Magnetic Thermistor Holder for LU CB 1 Thermistors BS4 65 0057 BSC T3 Bath Thermistor for Use with PDMI 2 PSMI LU CB1 and TC 202A BS4 65 0056 BSC T3A Bath Thermistor for use with i LU CPC CEH and TC 202A a BS4 65 0098 BSC T2 Replacement Bath Thermistor for Ad apters Obsolete TC 102 ome BS4 65 0016 BSC T2A Replacement Bath Thermistor for Use Bie E pa offers a i range 7 alignment rings as ar with LU CPC CEH and TC 102 rm a ACA a a a a E gejnge PDMI
171. mm P 2 BS4 64 0278 PH 2 BS4 64 0285 RC 21BRFS RC 25 95mm P 3 BS4 64 0279 PH 3 BS4 64 0286 RC 25F 13 0 mm P 4 BS4 64 0280 PH 4 BS4 64 0287 RC 20 20H 12 0 mm P 5 BS4 64 0281 PH 5 BS4 64 0288 i RC 27 27N 27NE RC 28 18 9 x 35 6 mm P 6 BS4 64 0282 PH 6 BS4 64 0289 RC 27L RC 29 18 9 x 35 6 mm P 6D BS4 64 0283 PH 6D BS4 64 0290 hy Ti nt A PA Heater Platform Cable Assemblies The CC 28 cable assembly is used to connect any Warner heater platform to a TC 324B or TC 344B heater controller The cable has two thermistors at the platform end the control thermistor which attaches to the platform and a bead thermistor for monitoring the bath tem perature The CC 28 is provided with a 9 pin din connector to plug into the heater controller An alternative cable the CC 15 is available with no inboard connector for users who choose to use another power source for 7 t T d d Ba a E i y m i y p Np p X i Heater Platform Upgrade Kit Specialized Tools For Bioresearch Non heater platforms can be converted to heater platforms with an upgrade kit consisting of a pair of resistive heaters heat transfer pads and mounting screws Tapped holes for the heaters are included in all plain platforms The only tool required for installation is a small Philips screwdriver The kit is ordered with either the CC 28 or CC 15 cable assembly Note Older models may not be upgradeable Consult our technical support depart
172. mosphere control DH 35i Incubators work well with Willco Wells Corning and Falcon glass bottom 35 mm cul ture dishes Adapter rings are supplied to ensure a close fit for the supported dish Dishes can be placed in the holder with or without dish covers The holder is machined in three pieces The midsection and top are easily removed for dish replacement and enables the positioning of electrodes at low angles Resistive heating of the DH 35i is provided via the optional Warner TC 324B Temperature Controller and a CC 28 cable Clamps are provided to ensure good contact between the dish and the heated surface and to prevent unwanted movement The removable midsection is magnetically secured and has several ports to allow multi channel perfusion atmospheric maintenance and aspiration of the dish The base of the holder has a 19 mm aperture and the removable cover features a 36 mm glass aperture DH 35i Micro Incubation System for 35 mm Cell Culture Dishes i w r gi m Adapter rings for Corning Falcon and Willco 35 mm dishes When combined with the Warner RC 37 Cell Culture Perfusion Chamber Insert a complete micro environmental system is achieved This system is compatible with Warner s SH 27B and SF 28 In line Solution Heaters Included with the system are an MP 2 Perfusion Manifold and a set of 4 culture dish adapter rings Stage adapters are available for all major microscopes Custom or modified stage
173. n of heat to just the chamber platform Since the platform applies heat only along the edge of a chamber a temperature gradient will be established due to the heat capacity of water coupled with flow of relatively cold solution into the chamber Uneven heating in the temporal regime can be generated by vary ing the rate cool perfusion solution is introduced into a heated plat form or by introducing drugs or solution into the perfusion path at a temperature different than that currently being perfused These considerations will be addressed in greater detail below Outgassing Another significant issue related to the application of heat to perfu sion solutions is termed outgassing Under standard conditions atmospheric gas in contact with a liquid solubilizes into solution and tends towards an equilibrium determined in part by the gas partial pressure and the temperature of the liquid Generally speak ing cold liquids are capable of solubilizing a greater volume of gas than a warm liquid Thus as the temperature of the liquid increas es dissolved gasses come out of solution resulting in the appearance of bubbles Heating Methods A number of approaches are used by investigators to regulate the temperature of a sample under study These include warming the chamber platform and or microscope optics using resistive Peltier or liquid based application devices channeling warm air across the imaging chamber and or optical components
174. nated with a 2 mm tinned copper wire for connection to your leads E208 Catalog No Model Product BS4 64 1311 E207 Sensor Tip 2 x 4 mm D x Thick Epoxy Tube 3 5 x 25 mm D x L BS4 64 1312 E208 Sensor Tip 2 x 4 mm D x Thick Epoxy Tube 5 x 50 mm D x L 11 000 Specialty Products to Enhance Your Bioresearch Reference Electrodes Reference Cells amp Ag AgCl Electrodes Reference Cells Ag AgCl electrodes are epoxy encapsulated in a plastic shell They can be used as preparation reference or ground electrodes Around 4 5 mm D they are available with 2 mm pin 2 mm jack and wire connection All have a Luer taper for convenient mounting in cham bers and other devices Catalog No Model BS4 64 1301 REF 1L BS4 64 1302 REF 2L BS4 64 1303 REF 3L Silver Wire Product Reference Cell with 2 m L Wire Reference Cell with 2 mm Pin Reference Cell with 2 mm Jack Made of annealed silver 99 99 pure Supplied for the convenience of researchers to fashion their own electrodes Catalog No Model BS4 64 1318 AG8W BS4 64 1319 AG10W BS4 64 1320 AG15W BS4 64 1321 AGT10W U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Product Silver Wire 0 20 mm 008 D 2 m L No Insulation Silver Wire 0 25 mm 010 D 2 m L No Insulation Silver Wire 0 37 mm 015 D 2 m L No Insulation Silver Wire 0 25 mm 010 D 2 m L Teflon Insulation Plugs and Jacks An assort
175. nd Stand Systems 1 Ch inNO System with inNO T BS4 69 3050 BS4 69 3051 BS4 69 3052 BS4 69 3053 BS4 69 0048 BS4 69 0049 BS4 69 0050 BS4 69 0051 Meter with Temp Compensation 2 Ch inNO System with inNO T BS4 69 3054 BS4 69 3055 BS4 69 3056 BS4 69 3057 BS4 69 3058 BS4 69 3059 BS4 69 3060 BS4 69 3061 Meter with Temp Compensation 11 000 Specialty Products to Enhance Your Bioresearch Cc Nitrate Reductor Nitrate The measurements of nitrate and nitrite anions in biological samples have gained more interest due to their association with the nitric oxide NO pathway Since these ions are the oxidation products of NO their presence can be used as an indicator of NO production activities There are many methods available for both direct and indirect measurement of nitrite Unfortunately this is not the case for nitrate due to its different chemical reactivity Therefore most of the analytical procedures involving nitrate include its conversion to nitrite using either enzymatic reduction or cadmium based reductors These conver sion procedures are tedious time consum ing expensive require multiple sample transfer and suffer from diminished activity Nitrite Our NEW Nitrate Reductor is specifically designed to overcome these shortcomings Principle of Operation Conventional bimetallic composition based reductors are based on copper plated cad mium beads The presence of copper metal
176. nds in liquids and 20 30 seconds in gases Harvard Apparatus now owns the Navicyte line of Ussing and Diffusion Chambers See pages M166 to M170 for complete details on those products and many others U S Toll Free 800 272 2775 e Fax 508 429 5732 Online www harvardapparatus com ASojolg 129 M9 Dissolved O FO ton Fiber Optic Oxygen Sensing System E Specialized Tools For Bioresearch M10 State S W Display Minimum Detectable Limits Dynamic Range Resolution Interference Resolution High O Concentration Low O Concentration Stability Drift Response Time Compensation Calibration Effect of Error in Calibration on Answers Replaceable Probe Probe Reconditioning Re Calibration Probe Temperature Range Storage Conditions Probe Lifetime Chemical Compatibility Dissolved Oxygen Gaseous Oxygen mmHg ppm mmHg ppm 0 44 mmHg 0 023 ppm 0 44 mmHg 0 06 0 to saturation level 0 to 100 0 11 to 0 37 mmHg for dissolved oxygen partial pressure 0 015 to 0 045 0 11 to 0 37 mmHg for oxygen partial pressure 0 to 160 mmHg gas 0 to 160 mmHg No interference from pH change or salinity No interference from CH acetone moisture or CO 0 02 ppm 0 4 0 003 ppm 0 01 lt 0 02 ppm per day lt 0 05 per day lt 1 sec lt 30 seconds with silicone overcoat lt 1 sec lt 30 sec with silicone overcoat For temperature only For temperature only 2 point calibration First
177. nt panel foot switch or external TTL pulse BNC Pressure Units p s i KPa switch selectable Pressure Monitor BNC connector 10 mV p s i Pressure Readout Inject balance clear output port Line Voltage 100 110 220 240 VAC Power Usage 220 W Meter Accuracy 0 1 full scale Foot Switches Inject fill hold and gated provided in plus and deluxe pkgs Weight 6 8 kg 15 Ib Dimensions HxWxD 11x 38x 25 5 cm 5 x 15 x 10 in Accessories Supplied Input output and holding hoses Model PLI 100 Product PLI 100 Basic Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord and Instruction Manual PLI 100 Plus Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord Instruction Manual Foot Switch Pipette Holder and Input Hose Adapter PLI 100 Deluxe PLI 100 Deluxe Pico Injector with Injection Balance Clear Filling and Holding Pressures Comes with Input Hose Output Hose Holding Hose Power Cord Instruction Manual Two Foot Switches Two Pipette Holders and Input Hose Adapter Catalog No BS4 65 0001 BS4 65 0002 PLI 100 Plus BS4 65 0003 Harvard Medical Systems PLI 90 Picoliter Injector The PLI 90 Pico Injector like the reliable PLI 100 Pico Injector see following pages controls the precise and reproducible regulation of injec
178. nt to 50 C without water cooling Temperature Stability 0 2 C with TC 202A at 37 C with 1 ml min perfusion Temperature Gradient 2 C with 1 5 ml media volume 1 ml min perfusion Across Chamber Built In Temperature Sensor Thermistor 100 k Q at 25 C YSI 44011 Peltier Device Current Rating 6 A max Media Perfusion Rates Up to 3 0 ml min Overall Dimensions H x D 17 x 152 mm 0 67 x 6 0 in Weight 0 5 kg 17 9 oz Microscope Stage Mounting Call Harvard Apparatus for options Catalog No Product BS4 65 0043 PDMI 2 Open Perfusion Micro Incubator comes with LU PD and LU ASP Adapter not included see page M93 BS4 65 0058 Copper Cooling Tube for PDMI 2 For TC 202A Bipolar Temperature Controller see page M125 For CO pH Controller see page M144 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 29 M87 Chambers for 35mm Cell Culture Dishes oD Gre a laa cn i d A Oh gt a wn re x gt a w oF w O V A Fa rara N B a mi U 1 amp D Dp L pean iS x a 3 ail Modular Design The introduction of glass bot tomed 35 mm culture dishes extended their use into areas such as confocal microscopy flu orescence microscopy and high resolution image analysis The DH 35i Culture Dish Incubator is a system designed for imaging live cells in an open culture dish for easy access or in a closed dish for gas at
179. o Model Product The BTX Tweezertrodes are reusable tweezer style in vivo electrodes for drug or gene delivery in animals Tweezertrodes consist of a stan Eepee HE i P BEOCE I MMUCIAN dard 11 5 cm tweezer that has been modified with stainless steel cir u cular or disk electrodes at the tip The gap between the electrode BOFS ae he a V tam disks may be adjusted from under 1 mm to over 2 cm Tweezertrodes men come in two models Model 520 has an electrode disk diameter of 7 25449 0204 i TEESE OEREO Ane mm and Model 522 has a diameter of 10 mm Tweezertrodes may be 984 45 0165 520 KIT Includes Tweezertrode 7 mm BS4 cleaned with a mild detergent and sterilized with ethanol or ethylene OME aNd UANG BSAA RUR oxide These electrodes are connected to a pulse generator with the B94 45 0166 522 KIT Includes Tweezertrode 10 mm BS4 45 0119 and Cable BS4 45 0204 Note All Tweezertrode models require Connection Cable BS4 45 0204 Model 524 Connection Cable and are compatible with most BTX Electroporation Systems Applications Tweezertrodes may be used for many in vivo applications including gene and drug delivery Following localized or systemic injection of the molecule of interest the Tweezertrode electrode disks are used to grasp the tissue of interest An electroporation pulse is then given initiating pore formation and incorporation of the molecule into the cells of the tissue in direct contact with the electrode disks Suzuki
180. o Genetrodes In Vivo Genetrode tips are invasive gold coated and 0 5 mm in diameter The shafts are straight and 0 8 mm in diameter Model 508 Electrode Length 5mm Shaft Configuration long The BTX Genetrodes are needle style reusable electrodes that are used Model 510 for a variety of applications Genetrodes come in five models with each Electrode Length 10 mm model consisting of a pair of electrodes The Model 508 and 510 Shaft Configuration long In Ovo Genetrodes In Ovo Genetrode tips are non invasive gold coated and 0 5 mm in diameter The shafts are 0 8 mm in diameter and L shaped Genetrodes are straight electrodes with gold tips The Model 512 514 and 516 Genetrodes are bent L shaped electrodes with gold tips These electrodes are connected to a Model 465 Square Post Micrograbber M62 The Model 512 514 and 516 electrodes are all suitable for in ovo gene transfer studies Funahasi has used an electrode similar to Model 516 with an 800 Series for small area gene transfer in ovo An electrode sim ilar to Model 514 has been used with a BTX generator by Muramatsu in his in ovo studies He concludes that electroporation is superior to both lipofection and microparticle bombardment Note All Genetrode models require Genetrode Holder BS4 45 0203 and Cables BS4 45 0201 and BS4 45 0210 Model 512 Electrode Length 5mm Cable and interfaced with a pulse generator via a Model 5343 Banana Model 514 Electrode Lenath
181. o need to be of the highest quality and provide the necessary reliability accuracy and repeatability to insure proper results Harvard Apparatus Inc Harvard Apparatus manufactures and sells the complete range of product that are needed Medical Systems i fully microinject and or microperfuse lcci Meat NEAR an p pages M71 and M72 Harvard Apparatus Medical Systems NeuroPhore pages L2 to L8 Pressure Injection Systems pages M75 and M76 M70 11 000 Specialty Products to Enhance Your Bioresearch Microinjection amp Microperfusion Harvard Medical Systems PLI 100 Pico Injector es ng BS4 65 0001 PLI 100 Pico Injector The PLI 100 Pico Injector reliably delivers a wide range of volumes through micropipettes by applying a regulated pressure for a digitally set period of time Compressed gas allows the user to deliver desired volumes from femtoliters to micro liters while simultaneously holding a cell Whether you need to do large injections into capillaries or very small injections into mam malian nuclei the PLI 100 is well suited for your experiment The PLI 100 has become a favorite of prestigious national microinjec tion workshops like Cold Spring Harbor Laboratories and other researchers worldwide Other companies have tried to design similar systems but the PLI 100 remains unparalleled in terms of ease of use durability precision and cost ost Published Injector Researchers Say
182. oReady Electrode Polarizer Tip Volume 100u Designed specially for measuring NO released from Oo monolayer cultured cells Please refer to the guide below to soe you O 600 um tip diameter and large flat tissues select the best sensor for your application gt For additional assistance please call Exceptional sensitivity and Rugged membrane Harvard Apparatus Technical Support J baseline stability Highest sensitivity The selection guide below is based on design sensi tivity and economy For example amiNO FLAT and amiNO IV are the most sensitive but are also the most expensive The amiNO 600 is less sensitive but is more economical Very rugged membrane Low background flexible body Very durable Tip Volume 4ul amiNO Sensor Selection Guide Application First Choice s Second Choice s Measuring NO released from cells large flat tissue amiNO FLAT amiNO 600 or amiNO IV In vivo measurements and other insertion applications amiNO IV amiNO 600 Measuring NO in small volumes or restricted space amiNO 100 amiNO 7 amiNO 30 or amiNO 600 Measuring micro molar levels of NO amiNO 2000 amiNO 100 or amiNO 30 Following the decomposition of an NO producing compound amiNO 600 or amiNO 100 amiNO 2000 or amiNO IV Measuring very low levels of NO below 50 nM amiNO 600 or amiNO IV amiNO 100 Indirect measurement of Nitrate and Nitrite amiNO 600 depending on amiNO 100 or amiNO 2000 expected level Training new staff or students
183. ofile Large Bath Recording Chamber RC 26GLP 234 pl RC 26GLP is a low profile version of the RC 26G The bath area of the chamber has been milled to a height of only 2 mm The lower profile provides extra clearance for upright microscopes Bottom coverslip size is 22 x 40 mm Order with P 1 or PH 1 platform see page M112 Catalog No Model Product BS4 64 0236 RC 26GPL Low Profile Large Bath Recording Chamber Optional Slice Anchors BS4 64 0252 BS4 64 0255 SHD 26H 2 Slice Anchor for RC 26 2 mm SHD 26GH2 Slice Anchor for RC 26G and RC 26GLP 2 mm Other Anchor Weights and Spacings available see page M116 M105 Special Chambers Systems Series 20 Open Bath Recording Imaging Chambers TOP VIEW Reservoir Perfusion Inlet Port 9 Agar Bridge Wells SIDE VIEW L T Ty BATH DIMENSIONS ee 12mm enn oe Volume 170 pl Volumes are for 1 mm solution height Large Bath Chamber for Oocyte Recording RC 26Z 170 pl Similar to the RC 26 this chamber has two additional features for oocyte recording the dam height in the suction well is increased to 2 5 mm to provide an adequate solution level in the bath and a second agar bridge well has been added to accommodate bath clamp electrodes Bottom coverslip size is 22 x 50 mm Ordered with P 1 or PH 1 platform see page M112 Catalog No Model Product BS4 64 0237 RC 26Z Large Bath Oocyte Recording Chamber RC 26Z Perfusion Inlet Port Suction Rese
184. og No Product BS4 69 0150 PMP 107 Programmable Multi Barrel Pipette Puller BS4 69 0151 PMP 102 Programmable Single Barrel Micropipette Puller BS4 69 0154 Input Tubing with Connector BS4 69 0155 Output Tubing with Connector and 4 5 ml Vial BS4 69 0156 Output Tubing with Connector Only BS4 69 0157 Foot Switch BS4 69 0158 Remote Key Pad BS4 69 0160 Rack Mounting Kits with Handles BS4 69 0161 Unpulled 7 Barrel Pipette for BS4 69 0150 Only BS4 69 0162 Unpulled 4 Barrel Pipette for BS4 69 0150 Only BS4 69 0165 Pipette Holder for Single Barrel Pipettes BS4 69 0166 Holder for 4 Barrel Pipettes BS4 69 0167 Holder for 7 Barrel Pipettes Note see page M76 for details 11 000 Specialty Products to Enhance Your Bioresearch De Fonbrune Microforge Forge Assembly Unit shown without Stereo Zoom Microscope The De Fonbrune is the oldest and most respected name in microforging The De Fonbrune type microforge is the latest in a time honored series based on Dr De Fonbrune s original device It complements the precision applications of the de Fonbrune pneumatic micromanipulator pipette pullers and microinjection systems by producing a wide variety of microtools adaptable to micro biological cytological genetic microsurgical microphysical and similar investigations and tools as precise as your procedure It can be used to fabricate a wide range of microtools employing a variety of te
185. olled temperature you select The perfusant is removed via user supplied suction by an included height adjustable aspirator LU ASP Two independent channels of perfusant flow through the PSMI into the chamber via replaceable plastic tubing of low volume lt 100 pl to allow rapid switching of media via an upstream valve for pharmacological studies A separate gas inlet provides temperature controlled gas across the dish The TC 202A provides temperature control 5 to 50 C using the micro incubator warming cooling plate as the control point Lower temperatures approximately 10 C below ambient may be obtained by using an optional cooling tube The cover slip dish PS CSD consists of two 22 mm diameter cover slips held in a circular frame The slice sits on the top cover slip with the immersion objective reaching the fluid surface The bottom cover slip prevents condensation for optimal illumination and viewing To enable placement of the patch electrode entry angles as small as 15 can be achieved for slice access Specifications Chamber Type PS CSD Cover Slip Dish 0 2 C with TC 202A at 37 C with 1 ml min perfusion Across chamber 2 C with 1 5 ml media volume 1 ml min Temperature Stability Temperature Gradient Temperature Range 5 10 C below ambient to 50 C without water cooling Thermistor type 100 KQ at 25 C YSI 44011 Current rating 6 A max Built In Temp Sensor Peltier Device Media Perfusion Rates Up to 3 0
186. olt Input Connector Output Connector Power Requirements Physical Size H x W x D Shipping Weight Warranty 1 mA minimum Minimum Pulse Width 100 us Maximum Pulse Width 100 s Bipolar Mode Only BNC Banana Jacks 90 to 270 VAC 50 60 Hz 10 VA 8 9 x 20 x 25 4 cm 3 kg Two years parts and labor U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASojo1g 2D M111 Series 20 Chamber Platforms amp Stage Adaptors Series 20 Platforms Series 20 Platforms The platform functions as a base for the chamber and provides clamping to make a seal between the chamber and coverslip They are machined from aluminum and finished with a black anodize With the exception of the model P 2 and PH 2 platforms Series 20 platforms feature sliding hold down clamps which permit rapid replacement of the chamber The model P 2 and PH 2 platforms use a one piece clamp as shown above Sliding clamps used on most models permit easy removal of the chamber to change the coverslip Model P 2 see to left uses a one piece clamp secured with 4 screws Heater platforms are powered from a single channel of a Warner heater controller see page M126 Connection to the controller is made with the CC 28 cable assembly see below Series 20 Platforms Aperture Plain For Chamber Model Size Platform Model Heater RC 22 22C 24 24E 17 8 mm P 1 BS4 64 0277 PH 1 BS4 64 0284 26 26G 26GLP 26Z RC 21B 21BR 17 5
187. ominal total Perfusate Outlet LU ASP aspirator Gas Port 1 16 in barb Gas Superfusion Rate 0 5 to 2 0 L min Weight 17 9 oz 0 5 kg Overall Dimensions 6 5 x 4x 1 in 16 5 x 10 2 x 2 5 cm Catalog No Product BS4 65 0101 CSMI Chambered Slide Micro Incubator BS4 65 0045 TC 202A Bipolar Temperature Controller see page M125 BS4 65 0103 Copper Cooling Tube for CSMI For TC 202A Bipolar Temperature Controller see page M125 For pH Controller see page M144 11 000 Specialty Products to Enhance Your Bioresearch Special Chambers Systems LU CB1 Leiden Micro Incubator PSMI Patch Slice Micro Incubator 5 n rS RRE Sr NT aed in BS4 65 0044 PSMI T maT se ies ade SAt in AS The Patch Slice Micro Incubator System provides a complete solu tion to the challenge of electro physiological study of synaptic slice connections at physiological temperatures using low noise whole cell patch recording either with or without water immersion of the microscope objective in the recording medium e Small entry angles ideal for patch slice recordings e Shallow fluid level mini mizes pipette capacitance e Heats amp Cools me 5 to 50 C e Peltier heat pump e Maintains cell conditions for hours This micro incubator is a shallow annular assembly which surrounds the central slice holding dish Room temperature perfusant is deliv ered to the incubator which in turn delivers it to the dish at a precise ly contr
188. on of temperature channel and probe by manufacturer Values determined if using probes BS4 69 0201 and BS4 65 0426 in pO range of 0 to 150 mmHg at probe temperatures between 15 C and 48 C and at instrument temperatures between 15 C and 30 C Offset lt 1 mmHg offset drift lt 1 mmHg 120 hr Probe sensitivity drift lt 5 24 hr and lt 10 120 hr Temperature compensation error lt 5 Linearity error lt 2 mmHg Absolute error lt 0 5 C 9 V DC max 700 mA during fast battery charging EU and UK 240 VAC 50 Hz USA 110 VAC 50 60 Hz Water protected metal case IP64 3 x 11 x 24 cm 1 2 x 4 3 x 9 5 in 750 g 26 5 oz net LICOX MCB Oxygen and Temperature Probes Cat No Length of Sensitive Tip Area to Sensitive Area Model REVOXODE Oxygen Probes Distance from Tip BS4 65 0415 BS4 65 0418 BS4 65 0416 BS4 69 0199 BS4 69 0200 BS4 69 0201 Thermocouple Temperature Probe BS4 69 0202 C1 5 mm 7 mm C1 R 1mm 2mm Cia 5mm 7mm C1 G2 7mm 5mm C1 SB 5mm 5mm C1 G3 4mm 5mm C8 B 3mm 4mm Catalog No BS4 69 0180 BS4 69 0181 BS4 69 0182 BS4 69 0183 BS4 69 0184 BS4 69 0187 BS4 69 0185 BS4 69 0186 BS4 69 0188 BS4 69 0189 BS4 69 0190 BS4 69 0191 BS4 69 0192 BS4 69 0195 BS4 69 0196 BS4 69 0197 BS4 69 0198 BS4 65 0445 of Catheter Near to Tip lt 0 46 mm lt 0 46 mm lt 0 46 mm lt 0 8mm lt 0 65 mm lt 0 8 mm lt 0 8 mm of Catheter Near
189. on with Dr James Robl University of Massachusetts 2 Saunders JA and Bates G W Guide to Electroporation and Electrofusion Academic Press p 477 479 1991 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 129 M67 Electroporation Electrodes Specialized Tools For Bioresearch M68 Meander Fusion Chamber The BTX Meander Fusion Chamber is a microslide with a novel design specifically used for electro cell fusion This chamber is constructed of a conductive metal alloy which has been deposited in a finger like projection array on a glass microscope slide This method of manufacturing creates a highly precise working area The configu ration is designed to give direct viewing of a surface area This can be used for viewing dimmer formation during alignment while under a microscope The gaps are set at 0 2 mm Application The Meander Fusion Chamber generates a homogenous field and is used for cell fusion of microorganisms plant fungi yeast and mammalian cells Product Meander Fusion Chamber product 454 0 2 mm Gap on Glass Microslide pkg of 4 requires Cables BS4 45 0202 and BS4 45 0210 Square Post Micrograbber Cables product 465 Coaxial Connection Cable 10 ft product 461 Catalog No BS4 45 0107 BS4 45 0202 BS4 45 0210 Call for your local BTX Dealer or visit www btxonline com qu Flatpack Chambers Flatpack Cham
190. oparticle bombardment Aihara and BS4 45 0201 and BS4 45 0210 Miyazaki used these electrodes with an 800 Series to transfer genes into BS4 45 0164 516 KIT Includes Bent L shaped 1 mm murine muscle tissue significantly increasing expression levels over Electrode Gold Tip BS4 45 0117 DNA injection alone Holder BS4 45 0203 and Cables BS4 45 0201 and BS4 45 0210 In Ovo Applications Model 512 514 and 516 References 1 Salford et al Biochemical and Biophysical Research Communications 194 2 938 943 1993 2 Yamaguchi et al J Exp Med 172 291 293 1994 3 Nishi et al Cancer Research 56 1050 1055 1996 4 Muramatsu et al Biochemical and Biophysical Research Communications 233 45 49 1997 5 Aibara and Miyazaki Nature Biotechnology 16 867 870 1998 7 Muramatsu Mizutani and Okumura Anim Sci Technol Jpn 67 10 906 909 1996 8 Muramatsu et al Biochemical and BioPhysical Communications 230 376 380 1997 11 000 Specialty Products to Enhance Your Bioresearch Electroporation Electrodes CD Tweezertrodes Specifications Voltage Range 0 to 1 0 kV Pulse Length Range 10 usec to 99 msec in PBS Model 520 Tweezertrode Length 11 5 cm 14 5 in Electrode Diameter 7mm Electrode Material Stainless steel Model 522 Tweezertrode Length 11 5 cm 4 5 in Electrode Diameter 10 mm Electrode Material Stainless steel Generator Compatibility ECM T 820 630 830 2001 Catalog N
191. or Stirrer BS4 73 0118 Power Supply for Stirrer with Adjustable Voltage gs 115 VAC 60 Hz BS4 73 0119 Power Supply for Stirrer with Adjustable Voltage 230 VAC 50 Hz BS4 73 0120 PO Electrode Type 2000 100 BS4 73 0122 Oxystat Measuring System Controller Model 719 Includes Controller and to Connect pO Electrode with Recording Outputs for pO pO Drift and Volume 115 230 VAC 60 50 Hz BS4 73 0124 Square Wave Stimulator G270 for Biphasic Stimulation 115 230 VAC 60 50 Hz BS4 50 1932 Harvard Apparatus Thermocirculator 115 VAC 60 Hz BS4 50 1940 Harvard Apparatus Thermocirculator 230 VAC 50 Hz Optional Accessories BS4 72 2987 2 Channel Flatbed Recorder Economy Version 250 mm Chart Width BS4 73 1523 Plugsys Minicase BS4 73 0210 OPPM Oxygen Partial Pressure Amplifier Module For the direct detection of oxygen see page M6 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M17 Respirometry Precision Dissolved Oxygen Measurement and Respirometry Specialized Tools For Bioresearch M18 Strathkelvin Instruments dissolved oxygen respirometry systems are based on ultra precise microcathode oxygen electrodes The oxygen meters and interfaces are supplied with dedicated respirometry software A choice of respirometer cells with volumes from 50 microlitres to 3 ml are available to meet most biomedical research applications CED 6 Channel Oxygen System Model 928 J The 928 i
192. or clear up The cell holding output port can produce an adjusted gentle suction to hold a cell eject a pressure to push a cell or completely clear the holding pipette Another drain output port can simultaneously produce an adjusted suction to drain and clear out liq uid before the next channel micro perfusion The PM 4 8 can deliver different quantity agents and drugs from picoliters to continuous perfu sion All output pressures and vacuums can be regulated and can be dis played real time on the front panel display Previous pressure readings can also be recovered on the display for setting comparison Besides programmable timers and counters there are 16 programmable and savable injection perfusion sequences with 32 programmable steps in each sequence Each sequence can be repeated continuously or triggered manually The interval time between steps can be programmed for auto matic sequential cycle or manual trigger There are two foot switch con nectors for sequential step trigger and injection trigger A digital remote port can be connected to an optional remote key pad or be controlled by a computer With exceptional versatility and extremely precise control either the PM 4 or 8 is an ideal multi channel injection perfusion engine Connection Diagram for Extracellular Micro Perfusion and Intracellular Micro Injection Systems PM 4 8 Programmable Pressure Injector PM4 4 Channe Injection PMB 8 Channel Injection Pressure Outpu
193. or up to 1 mm Pin with Axon Patch Clamps and Microelectrode 1 mm diameter tubing See the reports referenced below for information on the threaded collar Amps prior to 2 1 95 microperfusion technique PE SERIES Holders Glass OD Catalog No Wire Pellet Body Style Port Connector mm Model BS4 64 1144 Wire Straight 1 mm Pin PESW A10P BS4 64 1145 PESW A12P BS4 64 1146 PESW A15P BS4 64 1371 PESW A17P BS4 64 1147 PESW A20P BS4 64 1148 BNC PESW B10P BS4 64 1149 PESW B12P BS4 64 1150 PESW B15P BS4 64 1369 PESW B17P BS4 64 1151 PESW B20P BS4 64 1160 1 mm pin PESW T10P BS4 64 1161 Threaded i PESW T12P BS4 64 1162 Collar i PESW T15P BS4 64 1373 PESW T17P BS4 64 1163 PESW T20P _ Specialized Tools For Bioresearch References D Nebr E and Eckert R Fast patch pipette internal perfusion with minimum solution flow Grinnel A D Armstrong D and Jackson M B Calcium and Ion Channel Modulation Plenum Press N Y 2 Cull Candy S G Miledi R and Parker I Single glutamate activated channels recorded from locust muscle fibers with perfused patch clamp electrodes J Physiology 32 195 210 3 Pusch M and Nebr E 1987 Kinetics of loading small cells with various compounds by use of patch pipettes Pflugers Archives Spring Meeting of the Physiol Ges 4 Tang JM Wang J and Eisenberg R S 1992 Perfusing patch pipettes Methods in Enzymol 207 176 181 M40 11
194. oration The high voltage cables are connected with a BTX Pulse Generator and the electrode head inserted into the dish or well containing the sample An electroporation pulse may then be delivered The entire applicator may be cleaned with mild detergent and the electrodes may be sterilized with ethanol and dried with acetone Applications The Petri pulser PP35 2P is designed for the electroporation of adherent cells in situ or as an alternative to cuvette electroporation for larger volumes or multiple cell samples The use of the electrode for the electroporation of adherent cells in situ eliminates the need for trypsinization or mechanical removal of cells from their erowth substrate This eliminates associated problems including low plating efficiencies following electroporation interruption of cell cycle and interruption of intercellular communication Mammalian Cell Protein Drug Electroincorporation The Petri Pulser PP35 2P supplements our Model 366 Petri Dish Electrode designed for 100 mm petri dishes as the ideal electrode for in situ electroincorporation of protein into mammalian cells This electrode has been used for electroincorpo ration of peptides and proteins in CHO cells4 and vascular smooth muscle cells Mammalian Cell Transfections Gene Therapy The Petri Pulser PP35 2P is capable of supporting transfection protocols developed for our larger Model 366 Petri Dish Electrode including portocols for BHK and HUVEC Th
195. pH electrodes have sim ilar response characteristics to standard size glass pH electrodes making them compatible with any standard pH meter The pH series of electrodes come in a variety of configurations to fit every need Micro CO Electrode Luer Cap Oxygen Solution SI i Electrode eeve ove 3 mm Steel Shaft Plastic Holder vate COz Membrane Tee Fitting This CO electrode is connected to the BNC connector of a standard pH meter by developing a calibration curve CO vs mV using the mV mode on the pH meter The calibrations curve can be generated by using two known concentration standards Product Micro CO Electrode Catalog No BS4 69 0499 Combination pH Electrode for Micro Wells Luer Cap Reference Electrolyte Plastic Holder Reference Electrode Coax Cable BNC 4 ft Shaft 1 5 in L 0 5 mm OD pH Electrode Tee Luer Fitting 0 75 mm OD This a miniature combination pH reference electrode has been designed to fit small perfusion chambers without effecting fluid exchange rates It does not require an external reference electrode The reference solution can be replaced The electrode is stored wet Response Time 10 sec Slope 55 mV per pH range of 1 to 14 Impedance 200 M Q Stability 0 05 pH per day Dimensions L x D 38 1 x 0 75 mm 1 5 x 0 03 in Product Combination pH Electrode for Micro Wells Catalog No BS4 69 0494 Single pH Electrode in 25 Gauge Needle P
196. page M125 Lower temperatures can be achieved approximately 10 below ambient by using an optional cool tube water carrying coil which attaches to the perimeter of the PDMI 2 Temperatures stabilize quickly throughout the operat ing range temperature uniformity across the chamber is excellent Optimal and Flexible Perfusion Two temperature controlled perfusion lines deliver perfusates through the micro incubator into the chamber a nominal rate of 3 ml per minute each Fluid level is maintained by placing an aspirator model LU ASP in the chamber Fluid height is easily adjusted via a simple screw mech anism a cleverly designed suction port eliminates fluid flutter thus mak ing aspiration quiet a magnetic base secures the aspirator to the micro incubator at a user selectable convenient location B PDMI 2 with TC 202A Temperature Controller Nis Not Included Excellent pH Control The PDMI 2 also provides gas flow over the top of the chamber for pH control and improved temperature uniformity The open design provides excellent mechanical and optical access to the media Low Noise Conditions A teflon well is provided with an Ag AgCl electrode at its base to allow for the formation of a salt bridge when making low noise electrophysiological recordings Specifications Chamber Options Corning 35 mm Petri dishes LU CSD MSC TD or MSC PTD Recommended Gas Flow 0 5 to 2 0 L min Temperature Range 5 to 10 C below ambie
197. pl The bath volume in these two models is the smallest of the open style chambers This design allows very fast solution exchange for a variety of applications including patch clamp and studies in electro physiology The small bath volumes are especially useful when using drugs in small quantities Both models are used with 22 mm square or 22 mm x 40 mm coverslips Order with P 1 or PH 1 platform see page M112 Catalog No Model Product BS4 64 0230 RC 24 Fast Exchange Recording Chamber BS4 64 0231 RC 24E Fast Exchange Recording Chamber For Patch Clamps see pages H2 to H5 For Oocyte Clamps see pages H20 to H26 Slice Anchor Kits available see pages M116 and M117 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 2D M103 Special Chambers Systems Series 20 Open Bath Recording Imaging Chambers M104 Recording Chamber TOP VIEW Agar Bridge Well Suction Tube Slot Bath Perfusion Inlet Port Suction Reservoir Volume 54 ul for 1 mm solution height Model RC 24N Fast Exchange Recording Chamber 54 pl e Redesigned to improve flow characteristics This chamber features the small est bath volume of the open style chambers This design allows very fast solution exchange for a variety of applications including patch clamp and studies in elec trophysiology Small bath vol umes are especially useful when using drugs in small quantities A 22 m
198. point in oxygen free gas or liquid second point in air or air saturated water or liquid 1 error in reading of 0 concentration standard 1 6 mmHg error in low DO concentration 0 mmHg error in high end 1 error in reading of high concentration standard 4 mmHg error in high concentration 0 mmHg error in low end Yes Yes Yes Yes Only when probe is replaced 80 to 80 C 110 C briefly No specific requirements Only when probe is replaced 80 to 80 C 110 C briefly No specific requirements 1 year 1 year 1 Unaffected by pH lt 10 or salinity changes 2 Safe with 50 methanol hexane CH4 sodium sulfite acids 3 Not recommended with strong bases pH gt 10 ethanol acetone acetonitrile and HF and BTEX solvents 160 mmHg or 8 4 ppm for water saturated with air 760 mmHg or 41 ppm at 25 C for water saturated with oxygen gt 760 mmHg for water supersaturated with oxygen Catalog No BS4 69 0479 Replacement Parts BS4 69 0452 BS4 69 0458 BS4 69 0459 BS4 69 0463 BS4 69 0464 BS4 69 0465 BS4 69 0476 BS4 69 0477 Accessory Parts BS4 69 0453 BS4 69 0454 BS4 69 0455 BS4 69 0456 BS4 69 0457 Product FO ton Fiber Optic Oxygen Sensing System 115 230 VAC 50 60 HZ Fiber Optic Oxygen Probe Stainless Steel Probe 1000 um core D Bifurcated Optical Fiber Assembly 600 um Splice Bushing Temperature Sensor Fiber Optic Spectrometer for Fluorescence Blue Light Source for USB F O SP OOI Sensors SW O amp
199. power cord Weight 6 8 kg 15 Ib Dimensions HxWxD 11x 38x 25 5 x cm 5 x 15 x 10 in Catalog No Model Product BS4 65 0004 PLI 90 BASIC Pico Injector with Injection Balance and Clear Pressures Includes Input Hose PLI IHN and Output Hose PLI OHN Handle Power Cord and Inustruction Manual Applications Basic setups that do not need suction BS4 65 0005 PLI 90 PLUS Pico Injector Basic Model and Acces sories Shown Above Plus One Each PLI FS Foot Switch PLI PH1 Pipette Holder and PLI IHA Input Hose Adapter 11 000 Specialty Products to Enhance Your Bioresearch Catalog No Model Accessories BS4 65 0029 PLI FS BS4 65 0030 PLI RM Hosing BS4 65 0006 PLI IHA BS4 65 0007 PLI IHO BS4 65 0008 PLI IHN BS4 65 0009 PLI OHO BS4 65 0010 PLI OHN BS4 65 0011 PLI HHO BS4 65 0012 PLI HHN U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Microinjection amp Microperfusion Harvard Medical Systems PLI 100 and PLI 90 Accessories Product Footswitch for Inject Hold Fill or Gate Order more than one if frequent use of footswitch is needed for more than one of these functions Frame to Mount PLI 90 or PLI 100 in 19 in Wide Instrument Rack Input Hose Adapter 1 4 in Male BS4T Pipe Thread Gas Bottle Regulator Fitting Connects with PLI IHO N Gas Input Hose Tygon Tubing 9 ft x 0 250 in 6 35 mm x 0 125 in 3 18 mm x 0 062 in 1 57 mm L x OD x ID x Wall Thickness Connects to
200. pullers Programmable Pipette Pullers see page M52 To reshape the pipette or to fuse the tip into a different geometry a microforge system is used A microforge uses a heating element microscope illumination micromanipula tors and microtools to change the shape of or contact fuse other elements to the micropipette pipette by heating certain sections of the micropipette to either soften for bending or melting fusing the micropipette Some types of microforges can also be fabricate metal and glass microtools Harvard Apparatus Inc manufactures and sells the complete range of high quality equipment and supplies to fabricate muicropipettes Microforges pages M53 to M54 For Premium and Clark Capillary Glass see pages M45 to M49 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com M51 Pipette Pullers Specialized Tools For Bioresearch M52 Programmable Pipette Pullers BS4 69 0151 CE The PMP 102 is a state of the art micro processor controlled horizontal pipette puller that has been designed to pull two identical single barrel pipettes at the same time The pipettes can be used as patch clamp elec trodes intracellular electrodes injection micropipette or micro needles The PMP 107 is similar to the PMP 102 but is designed to pull a single 4 or 7 barrel pipette Under program control the PMP 107 can auto matically heat twist and pull a multi barrel
201. r 3 PE 160 Tubing 10 4 24 x 50 mm 1 Coverslip pkg of 50 Platforms Used With 1 P 6D Plain Platform 2 PH 6D Heater Platform Catalog No Model Product TOP VIEW END VIEW BS4 64 0244 RC 29 RC 29 Chamber with Slice Support Accessories Spare or Replacements BS4 64 0273 SS 3 Slice Support BS4 64 0256 SHD 27LH 10 Metal Slice Hold 50 mm Down 1 mm BS4 64 0257 SHD 27LH 15 Metal Slice Hold x Down 1 5 mm Bath A 17 5 mm mm ikiii i BS4 64 0258 SHD 27LH 2 Metal Slice Hold Down 2 mm BS4 64 0259 SHD 27LP 10 Plastic Slice Hold Down 1 mm BS4 64 0260 SHD 27LP 15 Plastic Slice Hold Down 1 5 mm Perfusion Inlet BS4 64 0261 SHD 27LP 2 Plastic Slice Hold Suction Tube Hole Down 2mm Note RC 29 Chambers require Platform P 6D or PH 6D and Microscope Stage Adapter see pages M112 to M115 Ea Specialized Tools For Bioresearch Slice Hold Downs available in Kits see page M117 M110 11 000 Specialty Products to Enhance Your Bioresearch Special Chambers Systems Gi SIU 102 Stimulus Isolation Unit Bipolar High Current Designed for use with Warner imaging chambers with field stimulation electrodes the SIU 102 features optical isolation at the input and transformer isolation at the output Timing pulses can be controlled by any device capable of generating a TTL level positive pulse a computer with data acquisition for example Positive or negative pulses positive or negative DC and bipolar pul
202. r LU CSD or MSC TD 1 Small approx 23 80 mm OD and 1 Large approx 29 80 mm OD set of 2 Cover Slip Dish O Rings Replacement for MSC PTD 1 Small approx 20 80 mm OD and 1 Large approx 29 80 mm OD set of 2 Glass Cover Slips 1 for LU CSD or MSC PTD 0 13 to 0 17 x 25 mm Thickness x D pkg of 100 Glass Cover Slips 2 for LU CSD or MSC PTD 0 18 to 0 25 x 25 mm Thickness x D pkg of 100 Perfusion Aspiration Teflon Tubing Replace ments for PDMI 2 PSMI 2 ft L x 26 AWG pkg of 8 Silicone Rubber Plugs for LU CPC or LU CPC CEH set of 4 Replacement Output Cable to Connect TC 102 Temperature Controller to LU CB 1 or LU CPC CEH ASoj01g 29 M93 Special Chambers Systems Specialized Tools For Bioresearch M94 Confocal Imaging Chamber RC 30 Pressure Plate Wrench Pressure p Plate a Locating Holes Top Cover Slip 22 x 30 mm Bottom Cover Slip 22 x 40 mm Locating The RC 30 is a low profile chamber with special features for confocal imaging The chamber bath has a variable volume and fast exchange and is formed with a silicone gasket sandwiched between the top and bottom cover slips Pre cut 250 pm thick gasket forms are supplied one wide cut to use the full aperture window 17 7 mm D and a slot ted version for faster solution exchange Blank gasket forms are also supplied permitting customized bath shape required for the applica tion Gaskets with 375 pm
203. r to maintain a constant temperature and a unique window valve for filling rinsing or injecting solutions into the chamber For con tinuous flow of water use our 66 77 Peristaltic Pumps see section A Stronghold stand an Monitori 1g System shown with uded see pages 022 027 e Sample sizes as small as 600 ul e deal for biological applications e Measures oxygen inline or in sealed sample Catalog No BS4 61 0130 System Components BS4 56 5077 BS4 56 5002 BS4 56 4930 BS4 61 0249 BS4 61 0248 BS4 61 0138 BS4 56 5028 Component Dual Chamber Oxygen Measuring System Dual Channel Amplifier qty of 1 Standard Oxygen Electrodes qty of 2 Batch Flow Chamber Systems qty of 2 Magnetic Stirring Kits qty of 2 Dual Channel Stirring Controller qty of 1 Chlorider and Accessory Kit qty of 1 Electrode Accessory Kit qty of 1 Dissolved O Harvard Instech Biological Oxygen Monitoring Systems Oxygen Electrodes Our Clark style polarographic electrode measures 41 3 mm 1 625 in long by 3 2 mm 0 125 in diameter The cathode is an exposed section of 0 1 mm 0 005 in pure platinum wire in a fused glass seal To prepare the electrode a sheet of polyethyl ene membrane material is sealed with an O ring over a drop of KCI solution on the electrode tip The temperature coefficient is 4 1 C so tem perature stability in your system is important Each electrode includes a cabl
204. rdings is therefore possible Selection Guidelines The PLI 100 is available in three packages Plus Basic and Deluxe The basic unit is supplied with an input hose output hose holding hose power cord and instruction manual The plus unit also includes a foot switch pipette holder and input hose adapter The deluxe unit includes all the previously mentioned accessories plus an additional foot switch and one pipette holder For Clark Electromedical Capillary Glass see pages M47 to M49 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 2D M71 Microinjection amp Microperfusion Specialized Tools For Bioresearch M72 Harvard Medical Systems PLI 100 Pico Injector Specifications Input Gas Pressure 70 to 105 p s i 480 to 720 kPa 0 2 to 60 p s i 413 kPa regulated multi turn control Injection Pressure Balance Pressure 0 1 to 3 5 p s i 68 9 kPa regulated multi turn control other ranges available upon request Fill Vacuum Internally produced 12 0 p s i 82 kPa unregu lated Internally produced 0 to 3 in H O 0 to 0 75 kPa or 0 to 0 1 p s i regulated Holding Vacuum Clearing Pressure Input gas pressure unregulated Injection Timer 1 sec steps Pulse Width Injection Count Display 0 01 to 0 99 sec in 10 msec steps 1 to 99 sec in Digital 0 through 9999 Duration Mode Internally timed or externally gated Time Trigger Fro
205. reparation Custom configurations of the AMANI series of pH combination electrodes are also available please contact Harvard Apparatus Technical Sales Department for further details Specifications Model Tip Diameter Tip Length Construction Depth of Immersion Min Sample Volume Response Time Temperature Range Internal Reference Slope pH Range Storage Connector Electrode Holder OD x Length Lead Wire Catalog No AMANI 650 650 um 20 mm 0 8 in All Plastic lt 100 um 250 nL lt 3 sec 90 response 0 to 100 C Ag AgCl Nernstian 0 to 14 Wet or dry BNC 4 8 x 75mm 0 19 x 2 9 in 0 9 m 3 tt BS4 69 0020 AMANI 1000 1000 um 20 mm 0 8 in All Plastic lt 100 ym 500 nL lt 3 sec 90 response 0 to 100 C Ag AgCl Nernstian 0 to 14 Wet or dry BNC 4 8 x 75 mm 0 19 x 2 9 in 0 9 m 3 ft BS4 69 0021 AMANI 1000L 1000 um 75 mm 3 in All Plastic lt 100 um 500 nL lt 3 sec 90 response 0 to 100 C Ag AgCl Nernstian 0 to 14 Wet or dry BNC 4 5 x50 mm 0 18 x 2 in 0 9 m 3 tt BS4 69 0022 AMANI IV 1200 um 37 mm 1 5 in Plastic Stainless Steel 1000 um n a lt 3 sec 90 response 0 to 100 C Ag AgCl Nernstian 0 to 14 Wet or dry BNC 4 8 x50 mm 0 19 x 2 in 0 9 m 3 ft BS4 69 0019 Harvard Apparatus sells a complete line of pH Meters and Magnetic Stirrers See the General Laboratory Section O or v
206. rodes The base of the holder has a 19 mm diameter aperture Stage adapters are available for many microscopes see pages M114 and M115 DH 35 is used with the CC 28 Cable Assembly see page M126 For 35 mm glass bottom culture dishes see right Catalog No Model Product BS4 64 0110 DH 35 35 mm Culture Dish Heater BS4 64 0106 CC 28 Cable Assembly BS4 64 0350 AR 1 Adapter Rin for Willco Dish Stage Adapters for DH 35 Stage Adapter Microscope BS4 64 0291 SA NIK Nikon Diaphot TE200 amp TE300 BS4 64 0295 SA OLY 2 Olympus IMT 2 IX amp BX50WI BS4 64 0296 SA 20LZ Zeiss Axiovert BS4 64 0296 SA 20LZ Leica DMIRB amp DMIL For other microscopes please consult our technical support department For Single and Dual Channel Heater Controllers see page M126 e Highest quality construction sterilized e 1 Cover glass for microscope imaging e Glass covers complete bottom e Compatible with DH 35 Dish Heater see left Cover Glasses Model Catalog No Pouch Pack Dishes 20 Sterilized Dishes Pouch Glass Bottom Cell Culture Dishes High quality polystyrene dishes with No 1 cover glass bottoms are available in 35 mm and 50 mm diameters They are avail able in both pouch pack 20 pouch and individual blister pack 120 case All dishes are sterilized before packaging The 35 mm sizes are compatible with the DH 35 Dish Heater see left Dish Dimensions D x H Glass Aperture BS4 64 0757 D3512P 35 x 9 9
207. rvoir BATH DIMENSIONS 38 mm a mm I 12 5 mm Volume 413 pl Volume is for 1 mm solution height Very Large Bath Chamber RC 27 413 pl This chamber and its evolutions on the following pages feature a slotted shaped bath The rectangular bath of the RC 27 will accommodate large specimens or the insertion of coverslips up to 12 x 24 mm typically used for organotypic slice culture studies An adjustable spreader plate at the bath input provides a uniform solution flow across the bath The bottom coverslip size is 24 x 50 mm Order with P 6 or PH 6 platform see page M112 Slice anchor kit available see page M117 Product Very Large Bath Recording Chamber Model RC 27 Catalog No BS4 64 0238 11 000 Specialty Products to Enhance Your Bioresearch Special Chambers Systems Series 20 Open Bath Recording Imaging Chambers RC 27N RC 27NE ee VIEW TE H Bath Area T Perfusion Inlet Port Suction Well Perfusion Spreader Surface Inclined to Bath Both Sides SIDE VIEW Narrow Bath Chamber RC 27N and Field Stimulation Chamber RC 27NE Model RC 27N is a modified version of the RC 27 chamber with a narrower bath and smaller bath volume Laminar solution flow is more stable than in the wider bath models Model RC 27NE is for applications requiring field stimulation and is supplied with a pair of platinum electrodes The electrodes are installed along the two parallel sides of the bath Electrode
208. s M135 M148 see pages M149 M159 see pages M160 M165 see pages M166 M175 see pages M176 M185 see pages M186 M201 see page M202 www harvardapparatus com Nitric Oxide Specialized Tools For Bioresearch M2 NEW Dual Channel System Uses amiNO sensors which are up to 100 times more sensitive than others on the market today inNO T system is the first available NO system to have temperature compensation inNO T system virtually eliminates base line drift due to temperature changes Combination NO meter and data acquisition system Battery powered benchtop portable system 5 year battery life 24 bit A D converter for the widest dynamic range 16 000 000 to 1 User friendly recording playback and analysis Software compatible with Windows 95 98 2000 and NT Continuous recordings over 1 month System shipped complete with NO meter software 2 types of NO sensors and calibration kit Auto zero Computer chart recorder RS 232 interface Digital filter Menu driven commands C inNO T Nitric Oxide Measurement System BS4 69 3054 Dual Channel inNO T System BS4 69 3050 a 1 Channel inNO T System inNO T Nitric Oxide Measurement System The inNO T system is the most technolog ically advanced nitric oxide electrochemi cal detection system on the market today lt combines a nitric oxide configured potentiostat and a sophisticated software controlled data acquisition system
209. s a complete system for replicate e For closed chamber respirometry measurements of dissolved oxygen together with data recording and analysis by comput er Use of this system will result in consider able time saving in both respiration experi ments and in dissolved oxygen monitoring situations The system is comprised of a microprocessor based interface which accepts up to six 1302 microcathode oxygen electrodes and dedicated Windows software All operations are controlled from the computer screen and output of recordings in chart recorder format to a printer is available e Flow respirometry e Dissolved oxygen monitoring The interface provides the polarizing voltage to the electrodes amplifies the low level signals makes the A D conversion and feeds the resulting data stream to the computer The system is a virtual instrument therefore there are no front panel controls All opera tions are controlled by the computer An illuminated front panel dis play provides information on the activity of the interface and displays readings from the electrodes when they are in use The 928 Oxygen System may be used with the Model RC 650 6 elec trode Respirometer see page M21 Details of the software are shown on the facing page Software included Resolution with 1302 Electrode 0 1 of air saturated water Polarizing Voltage 400 900 mV anode connected to ground Power Requirements 100 to 250 VAC 50 or 60 Hz Dimen
210. s et al directed the electroporation of COS 7 in a 96 well plate format while Parham et al optimized transient gene expression in CHO CV 1 COS 7 HEK 293 and NSO Applications Exponential decay generators may be used for a variety of animal in vivo applications including drug delivery to pig carotid artery and transdermal delivery of large molecules Specifications Operational Status Interface Input Charge Time Voltage Range LV Mode HV Mode Capacitance Range LV Mode HV Mode Resistance Range HV amp LV Modes LV Mode Precision Pulse LV Mode HV Mode Internal self test upon start up Digital User Interface 110 V 220 V Universal 5 sec maximum 10 to 500 V 1 V resolution 50 to 2500 V 5 V resolution 1 uF 25 uF to 3275 uF 25 uF resolution 25 UF 50 uF 25 Q 1575 Q 25 Q resolution None setting to simulate alternative systems lacking resist ance control Over 8300 RC Time Constants 126 RC Time Constants HV Mode Safety Short Circuit Proof Maximum Pulse Delay 10 sec Amperage LV Mode HV Mode Dimensions H x W x D 6000 A limit 3000 A limit 14 x 31 8 x 31 1 cm 5 5 x 12 5 x 12 25 in This product meets the requirements of the European Communities Weight ne eae EC and complies with EMC Directive 89 336 EEC and 73 23 EEC Display A0 eee LED for product safety Controls Single Rotary Encoder with push button toggle between all set p i parameters Additional on of
211. sensors completely shielded from environmental electrical noise e All sensors work the inNO T meter as well as many other currently available meters e One calibration procedure simple easy NO expensive chemicals Using the latest state of the art combination NO sensor design the amiNO series of nitric oxide sensors increases the sensitivity of electrochemical nitric oxide measure ment by up to 100 times that of any existing electrochemical sensor on the market today They are the first NO sensors to break the 100 pA nM barrier see chart on page M4 This higher sensitivity greatly reduces any measurement errors due to baseline drift associated with temperature changes This baseline drift has been a particular concern with older technology sensors that do not have the enhanced sensitivity characteristics of the amiNO sensors Additionally the new amiNO series adds newly designed sensor types for application involving in vivo and large surfaces cultured cells Customized sensors are available upon request to meet specialized applications call Harvard Apparatus Customer Service for details Harvard Apparatus offers 4 different Nitric Oxide Measurement Systems Each system includes either a 1 channel or 2 channel inNO T Temperature compensated meter plus an BS4 69 0012 amiNO 2000 sensor BS4 69 0016 SensoReady Electrode Polorizer and a temperature probe for a complete listing of system components see char
212. ses are possible in con stant current or constant voltage delivery modes For bipolar delivery a positive pulse during the input pulse is followed immediately by a negative pulse of equal duration and amplitude Bipolar pulse widths from 100 ps to 100 seconds per each polarity are possible Output amplitude is set using a range mode switch in conjunction with a ten turn potentiome ter to set a percentage of the selected range Indicator lights keep the user apprised of the following High voltage Lights red when output voltage above 30 volts Compliance Lights when impedance across output is too high for current setting T gt 100 Sec Lights when pulse width input is longer than 100 seconds in Bipolar mode Ext Trigger Lights when a valid input is applied to the Pulse In BNC Catalog No Model Product BS4 64 1424 SIU 102 Stimulus Isolation Unit BS4 64 1425 CC 102 Cable male banana to 1 mm jacks for use with Warner field stimulation chambers Specifications Output Waveform Output Current Ranges Output Voltage Ranges Output Impedance Output Compliance Output Polarity Amplitude Control DC Current Pulse or Voltage Pulse 1 10 and 100 mA 10 and 100 V 10 V Range 1 KQ Voltage Mode 100 V Range 10 KQ 100 V Selected by Mode Switch Red connector is positive in any positive mode Red connector is negative in any negative mode Multi turn dial Input Pulse Requirements TTL level positive pulse 3 v
213. sions 275 x 258 x 117 mm 11 x 11 x 4 5 in Catalog No Model Product BS4 69 3000 928 6 Channel Oxygen System CED 1 2 Channel Oxygen Meter a This versatile new Oxygen Meter acts as both a stand alone oxy gen meter displaying and stor ing oxygen values or as an interface to respirometry or monitoring software running on a PC It accepts inputs from either one or two Strathkelvin 1302 microcathode oxygen electrodes and shows the oxy gen values on a large liquid crystal display The display will show units of either PO mm Hg torr KPa or concentration pmol l pg l mg l pl ml ml ppm or saturation When connected to the software supplied which is installed on a PC it can be used for either closed cell respirometry using the MT200 mitocell RC300 or RC350 respira tion cell or for flow respirometry using the FC100 flowcell or MC100 microcell Alternatively the software can be used to monitor dissolved oxygen as in isolated organ preparations Details of the software are shown on the facing page Software included e Operates with 1 or 2 oxygen electrodes e Interfaces to respirome try monitoring software or use as stand alone meter Use any unit of PO or oxygen concentration e Closed cell flow through respirometry and O monitoring software included 0 01 of full scale 0 01 Resolution Repeatability Logging Capacity 2000 readings Computer Interface USB1 Power Requirements 5V
214. ssary the use of immersion objectives in imaging pres ents a difficult problem for the researcher attempting to maintain temperature at the sample Since the objective is in direct contact with the solution or chamber and is placed immediately above or below the sample it presents a significant conduction pathway within the system Micro Incubation Heated objective collars are commercially available and are designed to minimize heat flux through the optics However many researchers indicate that small variations in temperature as the collar cycles off and on induces an oscillating shift in the focus plane In addition many researchers express concerns about the effects of deep cycling an objective between significantly different temperatures between uses Some investigators address this issue by maintaining the objective at a constant temperature even when not in use Heated Enclosures Another strategy currently in use is to encase the imaging chamber and microscope optics within a heated enclosure The main advan lage of this approach is that both the chamber and objective are main tained at a stable and uniform temperature Heat is usually applied to the environment by the introduction of warmed air to the enclo sure Some disadvantages are slow responses to changes in the ther mal set point possible introduction of airborne contaminates from the blower system and the requirement for bulky equipment on or about the imaging system
215. st species at high efficiencies relative to exponential decay electroporation This product meets the requirements of the European Communities EC and complies with EMC Directive 89 336 EEC and 73 23 EEC for product safety References 1 Fakbrai Het al PNAS 93 2909 2914 1996 2 Pasco David University of Mississippi Natural Products Center Personal Communication and Protocols PRO296 0298 PRO345 349 PRO351 355 1998 3 Takahashi M et al Leukemia Research 15 6 507 513 1991 4 Tsong T et al Biblthca Haemat 51 108 114 1985 5 Marrero M et al The Journal of Biological Chemistry 270 26 15734 15738 1995 6 Miramatsu et al International Journal of Molecular Medicine 1 55 62 1998 7 Nishi T et al Cancer Research 56 1050 1055 1996 8 Heller R et al FEBS Letters 389 225 228 1996 9 Cambell K PPL Therapeutics Roslin Scotland Personal Communication 1998 10 Cibelli Jose et al Science 280 1256 1258 1998 11 Meng J et al Biology of Reproduction 57 454 459 1997 12 Lin C et al Plant Physiol Biochem 35 12 959 968 1997 13 Saunders J Guide to Electroporation and Electrofusion 227 247 1991 14 BTX Protocols PRO182 PRO183 PRO336 PRO342 1998 15 Costaglioli P et al Current Genetics 27 1 26 30 1994 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Specifications Operational Status
216. supplied with 10 feet of PE 160 Polyethylene Tubing U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com Perfusion ee Inlet Port TOP VIEW 27 4mm Volume 105 ul Volumes are for 1 mm solution height Catalog No Model BS4 64 0365 RC 37WS BS4 64 0366 RC 37FS Accessories Replacement Parts BS4 64 0758 D3522P BS4 64 0762 D3522B BS4 64 0378 111 KIT BS4 64 1424 SIU 102 BS4 64 1425 CC 102 34 0 mm Volume 144 ul Product Chamber Insert Closed for Willco 35 mm Dishes Chamber Insert Closed for Falcon Corning 35 mm Dishes Glass Bottom Cell Culture Dishes pouch of 20 Glass Bottom Cell Culture Dishes pkg of 120 Silicone Grease Kit with Brushes and Pallets Stimulus Isolation Unit Cable male banana to 1 mm jacks for use with Warner field stimulation chambers M91 Chambers for 35mm Cell Culture Dishes Glass Bottom Cell Culture Dishes 35 and 50 mm Specialized Tools For Bioresearch M92 cu Culture Dish Heater DH 35 DH 35 heaters work with Corning 35 mm tissue culture dishes Corning p n 25000 and are designed for mounting on most microscopes The holder is machined in two pieces The top half is easily removed for replacement of dishes Dishes can be placed in the holder with or without dish covers The holder is supplied with two additional holder covers one of which has openings to allow for perfusion or insertion of probes or elect
217. t 4 Barrel Pipette Geran eee oR wz 7 E ee Fame Drain Out Liquid Hold Cell Pipette Pipette Single Barrel Injection Pipette Tested Cell Extracellular Micro Perfusion Connection db Injection Time Signals of 4 or 8 Channels Hold Cell Pipette ingle Barrel Injection Pipette Intracellular Micro Injection Connection U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASoj01g 2D M75 Microinjection amp Microperfusion Multi Channel Pressure Injection Systems Specialized Tools For Bioresearch M76 BS4 69 0152 4 Channel Pressure Injection System Below are the components supplied with each of the Pressure Injection Systems BS4 BS4 Pressure Injection System 69 0152 69 0153 Cat No Product Qty Qty BS4 69 0154 IP1 Input Tubing with Connector 1 1 BS4 69 0156 OP2 Output Tubing with Connector Only 6 10 BS4 69 0157 FSW Foot Switch 1 1 BS4 69 0164 PP 4 Pulled 4 Barrel Pipette 1 BS4 69 0166 HOLD4 Holder for 4 Barrel Pipette 1 BS4 69 0163 PP 7 Pulled 7 Barrel Pipette 1 BS4 69 0167 HOLD Holder for 7 Barrel Pipette 1 Power Cord 1 1 User s Manual 1 1 Output Channels Input Gas Pressure Clearing Pressure Clear Hold Pressure Injection Pressure Balance Pressure Fill Vacuum Hold Vacuum Drain Vacuum Repeatability Display Accuracy Timer Setting Range Counter Setting Range Savable Sequences Programmable Steps Remote Control Ports
218. t 5 859 327 Jan 12 1999 cu Petri Dish Electrode The Petri Dish Electrode is designed to be used with a 100 mm petri dish which functions as the electroporation chamber To perform electroporation or electrofusion the samples are placed in the petri dish and the electrode assembly is lowered into the dish The electrode assembly is available in a 2 mm gap size It contains parallel stainless steel electrodes which produce homogeneous fields The electrodes are designed to electroporate volumes ranging from 4 to 50 ml The Petri Dish Electrode can be used with all BTX electroporation or electrofusion systems Applications The 2 mm gap Petri Dish Electrode can be used for electroporation of tissues grown right in the petri dish Simply add the exogenous molecule of interest mix and electroporate Product 100 mm Petri Dish Electrode 2 mm gap 50 ml Catalog No Model BS4 45 0100 366 Call for your local BTX Dealer or visit www btxonline com 11 000 Specialty Products to Enhance Your Bioresearch Electroporation Electrodes qu Petri Pulser The Petri Pulser PP35 2P is a reusable electroporation applicator designed to fit into each single well of a 6 well plate or an individ ual 35 mm diameter petri dish The PP35 2P consists of an electrode assembly embedded in a polyurethane holder with high voltage electrode cables The thin electrodes are gold plated and designed to maximize the surface area of electrop
219. t Flux Heat flux pathways can be generally characterized as either conduc tive convective or radiative Traditionally the greatest effort is directed towards controlling conductive pathways followed by con vective pathways We define a conduction heat pathway as one wherein heat energy is transmitted through a continuous interven ing material These include the microscope stage or objective the perfusion solution the chamber platform and other physical ele ments of the working environment Heat flux through convective heat pathways are generally less significant but include evaporation or condensation and heat transfer due to the movement of air across the imaging chamber Radiative pathways are in compari son easily controlled and will not be considered here Space and Time Considerations in Uneven Heat Conduction We first consider sources of uneven heat distribution in the spatial regime These include poor heat conduction across the chamber bath or from the platform to the chamber Poor heat conduction across the bath is revealed as a temperature gradient from one location to another within the bathing solution A gradient of this type is usual ly induced by an immersion objective placed directly under the sample Heat flux through this pathway can be significant resulting in as much as a 10 C temperature change within 3 5 mm Another less dramatic temperature gradient can be generated in the bath by restricting the applicatio
220. t on page M4 Each system is offered in either 115 or 230 VAC A gas phase NO sensor is currently under development please call for details 11 000 Specialty Products to Enhance Your Bioresearch Nitric Oxide C inNO T Nitric Oxide Measurement System 20 mm Max 12 Max _ _ _ _ es 70mm 0 6 mm Diameter 2 mm Diameter 60 mm __ 60 mm Sharp Tip Leama en 1 5 mm Diameter 1 mm Long 7 um tip diameter Flat 2 mm tip diameter 600 um tip diameter Ideal for single cell Stainless steel aluminum body Very sharp metallic tip for measurements insertion penetration of tissue Metal alloy fiber Longest life NO electrode no extra needles or catheters Tip Volume 500ul user replaceable membranes are needed sensor is the needle Tough enough for training Higher sensitivity up to 100 new staff and students times more sensitive than other 30 um tip diameter Used to measure high levels commercially available sensors of NO as in drug solutions Sub nano molar detection limit For space restricted and macrophages measurements Special membrane survives Tip Volume 250ul i Metal alloy fiber numerous insertions Tip Volume 10ul Very stable low background Current Tip Volume 6ul 100 um tip diameter a i T Extremely stable background 0 5cm 0 5cm Resists breakage To be used in restricted areas and in vivo measurements Very consistent amp reproducible BS4 69 0016 Sens
221. tem cell research The Chemical Drilling Micropipettes are specially designed for use with PiezoDrills These disposable Micropipettes are individually sterilized and available in five bend angles They are manufactured in an independently validated clean room to FED STD 209E class 10 000 All processes are controlled to BSEN46002 and ISO 9002 resulting in ultra clean and uni form micropipettes Each Micropipette is individually packed in a Twista Pak that ensures easy damage free removal of the micropipette from its packaging Five Twista Paks are then sealed into a blister pack to ensure that the micropipettes remain sterile and protected during freight Micropipettes pkg of 5 Bend Angle Cell Holding Pipettes Cell Removal Pipettes 1 15 pm 20 pm 35 pm 0 BS4 72 6496 BS4 72 6506 BS4 72 7592 BS4 72 7597 BS4 72 7602 BS4 72 7607 BS4 72 6497 BS4 72 6507 BS4 72 7593 BS4 72 7598 BS4 72 7603 BS4 72 7608 BS4 72 6498 BS4 72 6508 BS4 72 7594 BS4 72 7599 BS4 72 7604 BS4 72 7609 BS4 72 6499 BS4 72 6509 BS4 72 7595 BS4 72 7600 BS4 72 7605 BS4 72 7610 BS4 72 6500 BS4 72 6510 BS4 72 7596 BS4 72 7601 BS4 72 7606 BS4 72 7611 Chemical Bend Angle Cell Injection Pipettes Stem Cell Injection Pipettes Drilling Pipettes 1 5 pm 8 pm 14 17 pm 18 21 pm 22 25 pm 10 pm 0 BS4 72 7582 BS4 72 7587 BS4 72 6486 BS4 72 6511 BS4 72 6491 BS4 72 6501 20 BS4 72 7583 BS4 72 7588 BS4 72 6487 BS4 7
222. tended to pull bevel or forge various types and styles of glass micropipette needles for ICSI IVE transgenics patch recording or other similar procedures involving the use of micropipettes in electrophysiological recording microinjec tion and microperfusion techniques The first step in the process involves the initial fabrication of the micropipette itself using either single or multibarrel capillary tubing This tubing typically ranges in size from between 1 and 2 mm OD and is usually made from borosilicate glass although other materials such as aluminosilicate or quartz are also used in some applications The capillary tubing is then placed in a micropipette puller where a point on the capillary tubing is brought to its softening point through the use of a heating device such as an electrical filament laser or gas flame Once the capillary lubings softening point temperature is reached a mechanical parallel pulling force is applied to each end of the capillary tube and it is pulled to the desired tip diameter and profile Depending on a number of preset variables such as capillary material type temperature filment type and pulling force numerous types of tip profiles and tip diameters can be fabricated Using this technique tip sizes as small as 0 02 um can be achieved Pipette pullers can range in complexity from a simplistic spring counter weight mechanical puller to highly sophisticated programmable microprocessor controlled
223. tion pressure and time Simplicity Because the PLI 90 features only the injection balance and clearing positive pres sures it is a lower cost alternative to the PLI 100 This simplicity makes it even eas ier to use than the PLI 100 Selection Guidelines The PLI 90 is ideal for the user who does not require vacuum for filling pipette barrels or if using a holding pipette for holding cells is not essential to your application The PLI 90 is available in two packages Basic and Plus The basic includes an input and output hose handle power cord and instruction manual The plus model is supplied with all of the same parts as the basic model but also includes a Footswitch Pipette Holder and Input Hose Adapter Specifications Input Gas Pressure 70 to 105 p s i 480 to 720 kPa Injection Pressure 0 2 to 60 p s i 413 kPa regulated multi turn control Balance Pressure 0 1 to 3 5 p s i 68 9 kPa regulated multi turn control other ranges available upon request Clearing Pressure Input gas pressure unregulated Injection Time 0 01 to 0 99 sec in 10 msec steps 1 to 99 sec in 1 sec steps Pressure Display Digital three and a half digits Duration Mode Internally timed or externally triggered Trigger Mode Foot or panel switch Pressure Readout Inject balance clear output port Line Voltage 100 110 220 240 VAC Power Usage 220 W Foot Switch es Optional inject and gating Accessories Supplied Input hose output hose and
224. to Root lt 0 6 mm lt 0 6 mm lt 0 6 mm 0 8 mm 0 95 mm 0 8 mm 0 8 mm Product LICOX MCB Oxygen Monitoring System 115 VAC USA LICOX MCB Oxygen Monitoring System 230 VAC Europe LICOX MCB Oxygen Monitoring System 230 VAC UK Rechargeable NiMH Batteries pkg of 4 Tough Protection Pouch for Outdoor Measurement Hand Made Real Leather Bag with Front Panel Flap Accessory Pocket Cable Protection and Strap Power Supply USA 110 VAC 40 60 Hz Power Supply Europe 240 VAC 40 60 Hz Power Supply UK 240 VAC 40 60 Hz Calibration Device for REVOXODE Oxygen Probes Double Metal Barrel with Temperature Sensor Not required if using BS4 69 0201 Probe Oxygen Probe Cable Blue 2 2 m 7 2 ft Temperature Probe Cable Green 2 2 m 7 2 ft Extension Cable for O Probe Blue 1 5 m 4 9 ft Extension Cable for Temp Probe Green 1 5 m 4 9 ft Analog O Signal Output Cable with BNC Plug Analog Temp Signal Output Cable with BNC Plug Analog O Signal Output Cable with 4 mm Plugs Analog Temp Signal Output Cable with 4 mm Plugs pO Probe Offset Test Kit 1 Glass Capillary with 10 Ampoules pO Zero Solution Probe Length Probe Description 200 mm Standard Use 200 mm Short Tip High Resolution 300 mm Standard Use Long 460 mm Long and Very Robust 150 mm PE Tube 3 cm Steel Tube Shaft 330 mm Y Connector for Thermocouple 126mm PE Tube 3 cm Steel Shaft For further information on the use
225. tus com M35 Electrode Holders Specialized Tools For Bioresearch M36 Q Series Holders QSW Bxxp QRW AxxN Q45W AxxP Compatible with Warner Patch Clamps PC 501 amp PC 505 and Axon Patch Clamps older style prior to 2 1 95 NOTE Holders without ports are not suitable for patch clamp recording Q SERIES Holders with 1 mm Pin Catalog No BS4 64 1105 BS4 64 1106 BS4 64 1107 BS4 64 0745 BS4 64 1108 BS4 64 0821 BS4 64 0822 BS4 64 0823 BS4 64 0978 BS4 64 0824 BS4 64 0841 BS4 64 0842 BS4 64 0843 BS4 64 1098 BS4 64 0844 BS4 64 1055 BS4 64 1056 BS4 64 1057 BS4 64 0968 BS4 64 1058 BS4 64 0861 BS4 64 0862 BS4 64 0863 BS4 64 1103 BS4 64 0864 Wire Pellet Connector i 11 000 Specialty Products to Enhance Your Bioresearch Glass OD mm 1 0 12 15 1 7 Model QSW A10N QSW A12N QSW A15N QSW A17N QSW A20N QSW A10P QSW A12P QSW A15P QSW A17P QSW A20P Q45W A10N Q45W A12N Q45W A15N Q45W A17N Q45W A20N Q45W A10P Q45W A12P Q45W A15P Q45W A17P Q45W A20P QRW A10N QRW A12N QRW A15N QRW A17N QRW A20N Body Styles Connectors Coupling Port Body Material Polycarbonate Straight 45 and 90 1 mm Pin 1 mm Pin Threaded and BNC Ag Wire or Ag AgCl Hybrid 2 mm diameter Polycarbonate Standard Glass Sizes 1 0 1 2 1 5 1 7 and 2 0 mm ME Electrode Holders Q Series Holders Q SERIES Holders with 1 mm Pin Continue
226. two baths Material Polycarbonate Small Bath Dimensions Length x Width 28 x 3 8 mm Specimen Area 20 x 3 8 mm Volume 111 pl per mm of solution height Large Bath Dimensions Size L x W 28 x 9 5mm Specimen Area 21x 9 5mm Volume 234 ul per mm of solution height Standard Accessories Supplied Platforms Used With 1 PE 160 Tubing 10 ft L 2 24 x 50 mm 1 Coverslip pkg of 50 1 P 6 Plain Platform 2 PH 6 Heater Platform Product Excised Patch Recording Chamber Note The RC 28 is used with either P 6 or PH 6 Heater Platform on page M112 and one of Stage Adapters on pages M114 and M115 Model RC 28 Catalog No BS4 64 0243 For Patch Clamps see pages H2 to H5 For Patch Clamp Capillary Glass see page M46 U S Toll Free 800 272 2775 Fax 508 429 5732 Online www harvardapparatus com ASojo1g 129 M109 Special Chambers Systems Slice Chamber with Atmospherics RC 29 This chamber is designed for slice studies requiring the presence of gasses in fixed proportion It is modeled after the RC 27L This chamber is supplied with a slice support It has two ports at the top of the chamber for the input of gasses You must select a slice hold down to complete your assembly See listing below Material Polycarbonate Bath Dimensions Size L xW 38 x 18 mm Specimen Area 23 x 18 mm Volume 629 ul per mm of solution height Std Accessories Supplied 1 SS 3 Slice Support 2 Chamber Cove
227. uce reagents rapidly The Leiden closed perfusion micro incubator uses a nichrome heating element which surrounds the chamber It requires the TC 202A Temperature Controller Micro incubator supplied with output cable and temperature probe Specifications Cover Slips 1 or 2 25 mm D Model CS GLASS 1 or CS GLASS 2 Distance Between 14 mm 0 55 in Cover Slips Temperature Range Ambient to 50 C with TC 202A Heating Element 2 0 Nichrome heating coil Fluid Inlets Outlets 4 0 83 X 2 36 in Other Inlets for Probes 4 0 83 X 2 36 in Dimensions H x D 21 x 60 mm 0 83 x 2 36 in Weight 60 g 2 1 02 Dish Material Eriflon and Stainless Steel Product LU CPC CEH Leiden Closed Perfusion Micro Incubator Chamber with Heating Catalog No BS4 65 0041 Silicone Grease Kit We have determined that an effective method of applying silicone lubricant used to form a seal between the coverslip and chamber is an artist s acrylic brush With that in mind we have put together a convenient package that includes a tube of Dow Corning 111 Valve Lubricant amp Sealant 2 and 4 brushes and several pallets to aid in applying the grease Catalog No Model Product BS4 64 0378 111 Kit Silicone Grease Kit with Brushes and Pallets Silicone Lubricant Sealing between coverslip and chamber is most often done with a high purity silicone lubricant such as stopcock or vacuum grease The Dow Corning 111 Va
228. ure Coefficient c 2 per C Catalog No Model Product BS4 69 3006 1302 Microcathode Oxygen Electrode BS4 69 3007 Oxygen Electrode Service Kit BS4 69 3008 Electrolyte Solution 125 ml BS4 69 3009 Polypropylene Membranes Box of 15 BS4 69 3010 FEP Membranes pkg of 15 BS4 69 3011 Electrode Jacket with Hole for FEP Membranes BS4 69 3012 Electrode Jacket without Hole for Polypropylene Membranes BS4 69 3013 O Rings Box of 25 1130 GD Mitocell Miniature Respirometer Model MT200 and MT200A e Miniature respirometer for mitochondria and cell suspensions e Integral magnetic stirrer e Glass chamber with volumes of 50 100 ul MT200 or 0 3 0 5 1 0 ml MT200A and good visibility of contents e Transparent polycarbonate plunger e Substrate and inhibitors may be injected directly into chamber This new Mitocell Miniature Respirometer has a chamber volume of only 50 pl and was introduced for measurements on mitochondria isolated from biopsy samples It can be used in any situation where sample size is limited The base section contains an integral solid state fixed speed magnetic stirrer The 1302 microcathode electrode BS4 69 3006 is inserted from beneath the unit and its projecting tip forms the base of the respirometer chamber The glass chamber unit is surrounded by a water jacket through which constant temper ature water is circulated The respiration cell has a polycarbonate plunger with a central cap
229. ust be free from contamination at all times By storing completed pipettes and partially made pipettes between oper ations the complete cleanliness of the micropipettes can be assured Because the covers are made of Pyrex glass the complete container with pipettes in situ can be dry heat sterilized ensuring contamina tion free pipettes The base accepts 21 pipettes Select from containers that holds either 1 0 1 2 1 5 or 2 mm OD pipettes Catalog No Product BS4 69 0114 Pipette Sterilization and Storage Container for 1 0 mm Pipettes BS4 69 0316 Pipette Sterilization and Storage Container for 1 2 mm Pipettes BS4 69 0317 Pipette Sterilization and Storage Container for 1 5 mm Pipettes BS4 69 0318 Pipette Sterilization and Storage Container for 2 0 mm Pipettes Specialized Tools For Bioresearch Harvard Apparatus now owns the Navicyte line of Ussing and Diffusion Chambers See pages M166 to M170 for complete details on those prod ucts and many others Harvard Apparatus now owns Hugo Sachs Elektronik The HSE isolated organ baths set the industry standard They can be found in the Isolated Organ and Tissue Section K M50 7 Barrel lontophoresis Micropipette Pre pulled Blank Fully pulled Micron tip The MS 7P Micropipette has been designed for iontophoretic use as well as pneumatic ejection of drugs The standard blank consists of seven barrels pyrex Omegadot tubing 1 5 mm outer diamet
230. vardapparatus com M63 Electroporation Electrodes Specialized Tools For Bioresearch M64 a 2 Needle Array Electrode e Y l i k 1 l The BTX 2 Needle Array Electrode is an in vivo style electrode specifi cally designed for muscle gene delivery The electrode consists of a reusable delrin 2 Needle Array Handle and sterile disposable two nee dle array assemblies The needle array assemblies are conveniently packaged in Six Packs allowing for guaranteed sterility and designed to fit conveniently in a sterile hood The needles themselves are made of medical grade stainless steel The 2 Needle Array comes in 5 mm and 10 mm electrode spacings The Model 530 Handle with the Model 531 10 mm gap 2 needle array assembly are recommended for larger muscle masses such as rat gas tronemus The Model 532 Handle with Model 533 5 mm gap 2 needle array assembly are recommended for smaller muscle masses such as mouse tibialis Other species as well as other tissues may be electropo rated with the 2 Needle Array Applications Muscle In vivo Gene Delivery IVGD Among the non viral techniques for gene transfer in vivo the direct injection of plasmid DNA into muscle is simple inexpensive and safe Aihara and Miyazaki 1998 showed that by combining electropora tion with DNA injection expression was enhanced 100 fold Their results demonstrated that gene transfer into muscle by electroporation in vivo is more efficient t
231. ve pressures allow the user to 1 Eject precise amounts of fluids 2 Create a balance pressure which prevents backflow into the micropipette following an injection 3 Clear a micropipette of material in it Unique Features The PLI 100 s important Balance Hold and Clear functions are fea tures not found on other microinjection units Balance In addition to the ejection pressure the Pico Injector offers a secondary balance pressure This adjustable pressure keeps a positive pressure on the injection pipette before and after injections This eliminates dilution caused by capillary action and aids in the prevention of clogging Wasteful continuous injection which often occurs when no separate balance pressure is offered by an injection device is avoided Fill Hold There are two built in vacuum generators to fill a micropipette from the tip and to hold suspended cells Filling the pipette from the tip is easier than back filling Suspended cells can be held with a second holding pipette The holding vacuum s range accommodates most cell types Clear A high pressure pulse can be used for clearing a pipette should it hap pen to clog This is particularly useful when working with pipettes sized for smaller volumes Electrical Connectors BNC type connectors are available at the front panel to ease integrating the Pico Injector with other equipment Synchronization of injections to other stimulations or reco
232. vette Rack BS4 45 0208 ASoj01g 2D Call for your local BTX Dealer or visit www btxonline com M59 Electroporation Products Specialized Tools For Bioresearch M60 CD ECM 399 Electroporation System The ECM 399 is an electroporation system specifically designed to produce the precise field strengths and pulse lengths required for the transformation of bac terial and yeast cells and transfection of some mammalian cells The ECM 399 utilizes the BTX Power Platform Technology design and novel digital user inter face The single dial encoder simplifies the operation of ECM 399 Portable con venient and user friendly are major features of this unit Applications Bacteria Yeast Transformation In the High Voltage HV Mode the ECM 399 is optimized to electroporate F coli and other bacteria and yeast for construction of cDNA libraries or mutagenesis Mammalian Cell Transfection In the Low Voltage LV Mode the ECM 399 is designed to deliver a pulse length of approximately 26 msec using the 4mm gap cuvette at a volume of 400 pl using conductive media such as PBS This protocol has been found to be ideal for transfection of lymphoid myeloid glial fibroblast and Cos 7 cells Protocols for these and other mammalian cells can be optimized by varying the voltage and or the pulse length Adjusting the voltage adjustment knob varies the voltage while the pulse length may be changed by varying the sample volume or buf
233. volume in the chamber is maintained constant since the additional vol ume flows away through an outlet filter The volume introduced into the Oxystat to maintain the set pO is used to calculate the oxygen consump tion of the cells A cannula permits withdrawal of samples for biochem ical analysis without interfering with the measurement of oxygen con sumption As an option there is provision for fitting a fiber optic into the chamber for simultaneous measurement of additional parameters such as intracellular Ca concentration or redox potential Two platinum mesh fittings inside the chamber can be used for electrical stimulation of muscle cells to experimentally increase the O consumption A sepa rate stimulator see below is required for this application Oxystat Measuring System Model 719 This system includes the Oxystat control unit and the pump It con sists of a titrator connected to the pO meter The volume is displayed and can be recorded Square Wave Stimulator G 270 It has been developed for the stimulation of isolated cardiomyocytes in suspension The main specifications are e Output voltage 50 to 250 V e Maximum output current 7 A e Square wave biphasic stimulation pattern e Stimulation width 10 to 255 psec e Stimulation frequency 0 1 to 20 0 Hz 11 000 Specialty Products to Enhance Your Bioresearch HSE HA Oxystat System Type 1000 pO2 mmHg S Iu peppy wnoA Time min Figure 3 Original recording from
234. with the Warner QE 1 Quick Exchange Platform and the DH 35i Culture Dish Incubator J Dr z TF 3 sn PeF d a Po X F Yi f j Pa J E E g r j F T 5 J d d r The QE 1 Quick Exchange Platform The Warner QE 1 Quick Exchange Platform is a versatile base for the RC 40 Series chambers as well as many popular 35 mm glass bot tom dishes Removable perfusion and suction tubes allow solution exchange in any of the RC 40 Series open bath chambers or 35 mm dishes The platform may be heated using any of the Warner temper ature controllers The QE 1 can be mounted on most microscopes by using the Warner Series 20 stage adapters See page M86 for more information on the QE 1 11 000 Specialty Products to Enhance Your Bioresearch Micro Incubation Series 40 Low Profile Chambers for 15 18 and 25 mm Coverslips BATH DIMENSIONS 40 mm RC 40LP 25 mm coverslip RC 41LP 18 mm coverslip Series 40 Low Profile RC 42LP Open Bath Imaging Chambers The Series 40 family of cham 13 3 mm bers was designed for research requiring fast exchange of cov erslips The chamber design consists of two parts a polycar bonate chamber and a 40 mm diameter aluminum base O 15 mm coverslip rings are used to seal the cover O slip to the polycarbonate cham ber and to hold the chamber 10 5 mm Bg securely in the aluminum base S Open bath models are designed Volumes are for for electrophysiologi

Cell Biology - Harvard Apparatus

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