SPE-04 Brochure
Contents
1. E 04 2 Specifications Maximam volume of sample Pre condition load sample elution with 4 solvents blow dry of sorbent fraction collection Pump flow rate 1 to 20 mL min Power supply Method functions Current Weight Dimension cm 34 x 42 x 35 cm width x depth x height 3 Ordering Information Part Number Price US SPE 04 01 Includes SPE 04 mainframe 24V power supply and ae user manual www promochrom com 8 PromoChrom Instruments SPE 05 SPE 05 multi function SPE SPE 05 multi function SPE is a flexible and versatile platform for automatic sample preparation It can perform multiple tasks offline SPE online SPE normal sample injection and online derivatization with controlled temperature 1 Features 1 1 Offline SPE It can work like above described SPE 04 for offline column cleanup and fraction collection When working in offline mode computer is not necessary Users can set up the instrument quickly using the 7 buttons 1 2 Online SPE In online mode the collected fraction is directly injected into an HPLC or LC MS for final determination The control software for online SPE is user friendly and is compatible with most HPLC site C1 with 1 elute C1 with 2 software load sample to C1 elute C1 with 2 blow air elute C1 with 3 The software uses methods and aa sequences for the automation It has Paa similar structure as Agilent pa Inject reactant Chemstation Users o2. PromoChrom Instruments SPE 04 SPE 04 Cleanup Station SPE 04 cleanup station is designed for automatic cleanup of biological samples Compared to SPE O1 SPE 04 has smaller sample volume and fraction volume and can process much larger number of samples per batch 1 Features 1 1 Easy operation The operation of SPE 04 is similar to SPE O1 It involves only 7 buttons Below are typical routine operation procedures e Select method e place sample columns and receiving containers on the tray e Press start stop button 1 2 Small footprint and computer free operation Like other SPE instrument from PromoChrom SPE 04 has a very small footprint and does not need a computer The elution solvents are placed on the top of the instrument The compact design helps to save precious laboratory space 1 3 Different flow rate for each elution steps Different flow rates can be set for column conditioning sample loading and elution Procedures that are not sensitive to flow rate e g conditioning and blow dry can use higher flow rate to save time 1 4 Automatic needle wash Sample needle is thoroughly washed before processing each sample The tubing is also continuously washed to avoid cross contamination 1 5 Upgradeable to advanced functions SPE 04 can be upgraded to add online SPE and online derivatization function It can also add function for auto sample injection for HPLC www promochrom com 7 PromoChrom Instruments SP
3. f HPLC can start LC clean needle easily understand the SPE 04 software The software can perform overlapped injection When HPLC is performing an HPLC run SPE 04 can start processing the next sample default txt In online mode SPE 05 can perform derivatization under controlled temperature This function is very useful for analysis of amino acids hormones and some pesticides www promochrom com 9 PromoChrom Instruments SPE 05 1 3 Direct sample injection SPE 05 can work as an auto sampler Therefore the HPLC does not need to have another sample introduction device This feature can reduce the cost of an HPLC system considerably 2 Working principle The following diagrams describe the structure and typical working procedures of the 3 in 1 model Wash Change a ondition Load needle sample cartridge solvents Typical Collect fraction Elute with working Offline SPE 4 1 2 solvents proce dures Inject to HELE Derivatize with 1 or 2 Collect fraction trigger to start reagents at controlled Online SPE HEL temperature Optional The plunger for SPE column can seal the column well It can work with SPE columns from most suppliers There is no need for a special cap or adapter 3 Application example Direct analysis of hormone in plasma sample 1 Dilute plasma sample with 1 phosphoric acid at 1 1 ratio 2 Precondition a 3 mL 500 mg C18 SPE column with 2 mL
4. methanol followed by 2 mL water 3 Load 2 mL sample and wash with 4 mL water methanol 80 20 4 Wash SPE column using methanol and collect 1 mL fraction O O So 60 C pH10 OC No H C CH www promochrom com 10 Estrone Dansyl chloride PromoChrom Instruments SPE 05 5 Derivatize the fraction with dansyl chloride at 60 C 6 HPLC analysis using a PCTsil C18 column and UV or fluorescence detection 4 Specifications Pre condition load sample elution with 5 solvents blow Method functions dry of sorbent mix fraction with 1 or 2 derivatization reagents heat up to 80 C Temperature for derivatization Ambient to 80 C Type of derivatization reagent 2 Dimension cm 5 Ordering Information Part Number Price US Includes SPE 05 mainframe sample injection SPE 05 01 module for HPLC control software remote cable for HPLC 24V power supply and user manual Includes SPE 05 mainframe sample injection SPE 05 02 function for HPLC online derivatization module and control software remote cable for HPLC 24V power supply and user manual www promochrom com 11
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