Confocal Wire Myograph System - 120CW
Contents
1. 13 2 2 OCIS UP S SLC DD u Suasana aakasqasqawqasqayqasayawpuqawaqwquqqaqaaqaqqanQhuaquqkaayawaqkasuaqQhaqpaaqku qusa anqasstysqaussuiqayQunau qasqa 13 2 3 The Store transducer calibrati u ULU ii lili 14 Chapter 3 The Myo Interfaces J UU u u UU KE U U Q Q J J 15 O Tte Onhe VINO TPM La 15 sine DD i eU Mmm 15 Chapter 4 The confocal Wire Myograph unit J eren enne n nnam nhan a eaa u RES SNR RRRR IRR RR Q U J J T 18 4 L Adustiment of SuUDDOP E ated 18 4 A Macc netted cette Hm 19 Pe We Cie We Ar n nie RETEA EEEE 22 4 4 Changing myograph WI dow 1 66 u aa DN daisi rias kad 29 ESTE al u u u A A AN E E A Xr IAM qpuawupa asspaspakasawaqaqu 23 Appendix 1 Fuse replacement2. sesseasoosooseoseooseosseossaossosoossosoossos ON OFF switch RENO Se e Power connector REGUEATOR RS232 MYOGRAPH Regulator port for electronic Connection port for 120CW valve optional Myograph unit RS 232 port for serial connection to PC Connection port for pH Meter optional USE part as e 12VDC port Figure 1 3 Myo Interface rear panel 12 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL CHAPTER 2 SETTING UP 2 1 The complete Confocal Wire Myograph System 120CW PC data acquisition and analysis Power supply PowerLab data acquisition system software optional Myo Interface front panel i optional B T BNC Cables Myo Interface rear panel i Power supply pH meter optional Myograph unit Vacuum pump Electronic valve optional o optional Suction bottle Connection to oxygen supply Figure 2 1 The complete Confocal Wire Myograph System 120CW 2 2 Setting up step by step The chapter contains a complete step by step description of how to set up a complete myograph system as illustrated in figure 2 1 above 1 Myograph unit Myo Interface connection Connect the myograph 120CW unit to the Myo Interfa
3. J U U FER VM EM EMI EN CF u Q J J J J J 26 Appendix 2 System specifications sblesucesniwubiiessvandsbninutunts 27 4 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL INTRODUCTION The Confocal Wire Myograph System 120CW is specially designed to provide very close optical access to the mounted artery segment High resolution images of fluorescent dyes or markers within the living tissues such as those by laser scanning micros copy LSM become possible Combining LSM technology with myography allows simultaneous measurement of isometric force and dynamic intracellular events such as changes in intracellular Ca or pH occurring in living cells in the wall of isolated blood vessels The unique design of the myograph combines the precision and stability of our conventional wire myographs with the added fea ture of precise Z axis movement with a micrometer This optimizes the use with different LSM s and various high magnification and high numerical aperture objective lenses The conical bath design on the underside of the chamber allows the high numerical aperture lenses used on inverted micro scopes and also direct immersion lenses used on standard upright microscopes to come directly in cont
4. and use the correct type See the table below Voltage Line plug according to standard 110 125 V UL817 and CSA C22 2 No 42 220 230 V CEE 7 page VII SR section 107 2 D1 IEC 83 page C4 240 V BS 1363 of 1984 Specification for 13A fused plugs and switched and unswitched socket outlets Protect the power cord from being walked on or pinched particularly at power plugs and the point where they connect to the apparatus Refer all servicing to qualified service personnel Servicing is required when the apparatus has been damaged in any way such as the power supply cord or plug is damaged liquid has spilled onto or objects have fallen into the apparatus the apparatus has been exposed to rain or moisture does not operate normally or has been dropped 6 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL EMC EMI This equipment has been tested and found to comply with the limits for a Class B Digital device pursuant to part 15 of the FCC rules These limits are designed to provide reasonable protection against harmful interference in residential installations This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instructions may cause harmful interference to radio communications However there is no guarantee that interference will not occur in a particular installation If this equipment does cause harmful interference to radio or television reception which
5. required and without any notice Copyright Danish Myo Technology A S DANISH MYO TECHNOL Oct TRADEMARKS 3 CONTENTS 5 COM AEM vw su Rea RR EZ 3 MUNN ON oyua 1 uuu re sae eee ates E EEN ie nc we eee cto ie ee RON 5 27 027 u uwa I o Po 6 EMC 7 m 7 PADOVA e 4 Certificate of Conformmiy 2 uU ia RE AE EVER EV RR DN ERE ADR 8 ADOUT this manuak Meme 9 Unpacking the myograph system U U U U nnmnnn nnmnnn na 10 Chapter te Sys e Ai TIT E EEE 11 L 1 COontocal Nd esce E mmc 11 1 2 Myo intoriace Mont pane a ista 12 1 3 Myo Interrace rear panel 12 Cha pir z s UIO i u u u 13 2 1 The complete Confocal Wire Myograph System 120CW
6. supports is performed using the following step by step procedure Please note that the amount of force on the screws should be very little to avoid breaking the threads The procedure is illustrated in figure 4 1 on next page 1 Carefully loosen screw A on the top of the support connected to the force transducer Align the horizontal support and carefully tighten the screw again 2 Loosen screw B on the top of the support connected to the linear slide Align the horizontal Support matching the force transducer connected support as carefully as possible and gently tighten the screw again 3 Loosen screw C on the linear slide to roughly match the linear slide support to the force transducer support in the horizon tal plane as illustrated in figure 4 1 on next page Tighten the screw before proceeding with step 4 4 The plate on which the linear slide is mounted is balanced on top of a small stainless steel ball making it possible to finely adjust the linear slide Support in all vertical and horizontal planes using the four Allen screws D Use the four Allen screws to make the final horizontal and vertical figure 4 1 on next page adjustments to match the linear slide Support to the force transducer support The correct matching of the supports is illustrated in figure 4 1 1 on next page IMPORTANT BE CAREFUL NOT ONLY TO CONTINUOUSLY TIGHTEN THE ALLEN SCREWS WHEN DOING THE FINAL ADJUSTMENTS ALWAYS REMEMBER TO LOOSEN THE ALLEN SCREW PLACE
7. take care of the problems as soon as possible If the packing material appears damaged please retain it until a possible claim has been settled We recommend that you store the packing material for any possible future transport of the Confocal Wire Myograph System In case of transport and the original packing material is unavailable please contact DMT Sales Department for advice and packing instructions After unpacking your new Confocal Wire Myograph System please use the following list to check that the system is complete 1 Myo Interface unit e Myograph connection cable with a temperature probe Power cord The shape of the AC plug varies by country be sure that the plug has the right shape for your location e Automatic voltage selector 2 Confocal wire myograph unit Calibration kit including bridge balance and 2 g weight Chamber cover e Vacuum valve manual 3 Accessories roll of 40 um stainless steel wire e tube of high vacuum grease e 1tube of grease for linear slides e 4 spare screws for mounting of jaws e 10 extra myograph window glasses e 2 Allen keys e 1 small screwdriver e 40 mm funnel e Serial cable 4 Manuals e 1 CD with user manuals for Wire Myograph Systems e 1 CD with the manual Procedures for investigation of small vessels using small vessel myograph by Professor M J Mulvany Department of Pharmacology Aarhus University Denmark and the video Dissection and mounting of small vessels in
8. the F keys to proceed with the following options or press Info again to return to the Main menu F1 Change to the next main menu Recorder F2 Opens the Valve Activation submenu An optional electronic vacuum valve is available for the system which can be activated here F3 Opens the Zero Force submenu press F1 to zero the force or F4 to cancel F4 Displays a condensed Main menu Press F4 to toggle between the condensed and the normal view CHAPTER3 15 Recorder menu The 120CW Myo Interface has four analog output ports on the front panel for connection to a data acquisition system These default settings for these output ports are Rec 1 Myograph force FORCE output Rec 2 Temperature TEMP output Rec 3 pHreading output if installed Rec 4 No output The output order can be programmed to whatever order you desire Make the recorder line of interest active uppermost in dis play and use the Enter key to toggle between the available signals The full scale output from the Myo Interface is one volt In the Recorder menu it is possible to change the associated values for each analog output that correspond to O V lo and 1 V hi Timer menu The Timer menu makes it possible for the Myo Interface to act as a timer the interface can sound an alarm after a predefined countdown To set the countdown time move the line to be programmed hour minute or second to the top of the display Use the numeric keys to enter the time value
9. the wire and the gap in the support Make sure that absolutely no force is applied on the force transducer by checking that the tip of the transducer arm is not touching either the wire or jaw Also check that the relative force reading in the display is stable Press F3 to proceed with calibrating Carefully place the 2 g weight on the pan as illustrated in figure 4 3 on previous page and figure 4 4 above The force applied on the force transducer should mimic the stretch created by the contraction of a mounted ring preparation Wait until the relative force reading is stable Press F3 to finish the calibration Press Esc and go to the Main menu on the Myo Interface The force reading on the Myo Interface should now be very close to 9 81 mN Carefully remove the weight and proceed from step 3 to calibrate the other myograph If the force reading is different from 9 81 mN then try to calibrate the force transducer once again starting with step 3 After calibrating carefully remove weight balance and calibration bridge The myograph is now ready for use 4 3 Checking the force transducer The myograph force transducer is a strain gauge connected in a Wheatstone bridge The force transducer is placed in a separate compartment on top of the Wire myograph unit The separate compartment provides some mechanical protection for the force transducer but the transducer is still very vulnerable to applied forces exceeding 1 newton 100 gram or fluid runn
10. D DIAGONAL TO THE ALLEN SCREW BEING TIGHTENED OTHERWISE THERE IS A HIGH RISK OF DAMAGING THE MYOGRAPH FRAME Special designed jaws The myograph are supplied with jaws for inverted scopes but can be used with normal stainless steel jaws to mount small vessels on an upright system For experimental work requiring electrical field stimulation a set of plastic jaws with attached platinum electrodes is available Chamber The chamber has a conical shape and can thus be used with a small amount of buffer liquid The chamber can be customized to operate with volumes of 3 mL or less The conical milling on the bottom outside of the chamber allows objectives on inverted microscopes to be raised to the thin glass window to accommodate very low working distance objectives 18 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL A Screw on support connected to the force transducer C Screw on the linear slide E D Four Allen screws 2 B Screw on support connected to the linear slide Figure 4 1 Illustration of how to adjust the supports Figure 4 1 1 Illustration of the reverse supports CHAPTER4 19 4 2 Calibration of force transducer As a part of the general maintenance of the myograph DMT recommends that the myograph is weight calibrated at least once every month DMT also recommends that the myograph is weight calibrated every time the system has been moved or has not been used for a long period of time 4 2 1 Weight cal
11. DANISH MYO TECHNOL Ost CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW 3 pne Tm 1 no an E Da IBN t L User Manual Version 3 5 2 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW TRADEMARKS PowerLab and LabChart are registered trademarks of ADInstruments Pty Ltd The names of specific recording units such as PowerLab 4 25 are trademarks of ADInstruments Pty Ltd Pentium is a registered trademark of the Intel Corporation Windows Windows 95 Windows 98 Windows ME Windows NT Windows 2000 Windows XP and Vista are registered trademarks of Microsoft Corporation All other trademarks are the properties of their respective owners DMT reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately This documentation is provided with the DMT Confocal Wire Myograph System Model 120CW Document Number 120CW UG3 3A All rights reserved No part of this manual may be reproduced or transmitted in any form or by any means without the written permission of Danish Myo Technology A S Every attempt is made to ensure accurate information misprints construction and specification changes can occur Danish Myo Technology A S reserves the right to alter change content as
12. act with the cover slip of the chamber window Also by using special mounting supports designed specifically for vertical positioning an isolated blood vessel can be positioned directly above or on the chamber window This permits use of lenses with working distances smaller than 250 um on an inverted LSM This may be advantageous for simultaneous electrophysiological measurements The chambers are supplied with custom covers for inverted or upright microscope systems with connections for suction gassing or measurement electrodes pH NO O tension The myograph are supplied with jaws for inverted scopes but can be used with conventional jaws on an upright system For experimental work requiring electrical field stimulation a set of plastic jaws with at tached platinum electrodes is available During the experiment the circumference of the vessel is kept constant i e the vessel is examined under isometric conditions Compounds are added directly to the chamber and the resulting contractile changes in vessel force tension are measured While on the LSM data acquired from the myograph such as force and temperature can be recorded continuously either through the serial interface output or with the Myo Interface analog outputs connected to an external data acquisition system INTRODUCTION 5 SAFETY The confocal wire myograph system has been designed for use only in teaching and research applications It is not intended for clinical or critical li
13. and press Enter to confirm To control the timer function press the Info key and the display shows the F key options for the Timer menu Choose one of the F keys to proceed with the following options or press Info again to return to the Main menu F1 Proceed to the Setup menu F2 Start the countdown the time values are entered in the Timer menu F3 Stop or pause the countdown FA Reset the entered time values or to turn off the alarm F1 MAIN MENU Force OLS nN Acttemp 36 8 C RECORDER MENU Rec 1 SAORGE Recllo 20 Reclhi 20 Rec 2 TEMP Rec2lo 0 Rec2hi 50 Rec 3 Nouse Rec3lo Rec3hi Rec 4 Nouse Rec4Alo Rec4hi SETUP Calibrate FORCE Valve dly 5 Contrast 35 Baudrate 9600 Option 0 Settemp 37 0 C Heatis ON Change menu Start timer Stop Pause Reset Info FA F1 Change menu Change menu F2 Valve ON F3 Zero force F4 Condensed menu Calibrate Force 2 Activate Valve F3 Yes F1 No 4 FA CALIBRATE FORCE F3 Apply no force Push F3 when stable F Zero force MS 2 Yes F1 No F4 Force 0 15 mN Acttemp 36 8 C CALIBRATE FORCE Place 2 gram on pan Push F3 when stable Force 1880 Settemp 37 0 ON Calibrate Force DONE Push Esc Force zero 17157 Force gain 168 9 Figure 3 1 Menu overview the gr
14. block 3 Remove the existing fuses 4 nsert the new fuses 5 Replace the fuse block back into the voltage selector block NOTE ENSURE THAT THE CORRECT VOLTAGE FOR YOUR COUNTRY IS DISPLAYED Voltage selector block Red fuse block e APPENDIX 1 25 APPENDIX 2 SYSTEM SPECIFICATIONS Technical specifications Vessel size Vessel alignment Chamber Chamber material Chamber volume Chamber suction Chamber cover Chamber gassing Force range Force resolution Micropositioners Weight calibration Heating Temp range Temp resolution Temp probe Output reading Analogue output Serial output Voltage Ambient temp gt 60 um Manually X Y amp Z settings Single bath conical shape Acid resistant stainless steel Max 10 ml NO With connections for suction and gassing Yes 100 to 200mN O 1mN Manually operated precision micrometers Manual Built into chamber independent of superfusion Ambient temp 50 C 0 1 C External Force mN Up to four outputs 1 full scale for all acquired signals user defined Serial interface RS232 RS485 100 to 240 VAC auto 50 60Hz 15 30 C Optional accessories Enable pH meter on the interface range pH temp correction 0 14 0 50 C 26 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL NOTES NOTES 27 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 00 mee a
15. can be determined by monitoring the interference while turning the equipment off and on the user is encouraged to correct the interference by one or more of the following measures e Reorient or relocate the receiving antenna e Increase the separation between the equipment and receiver e Connect the equipment into an outlet on a circuit different to that which the receiver is connected to e Consult the dealer or an experienced radio TV technician for help APPROVALS Complies with the EMC standards 89 336 EEC EN 61326 2 6 2005 EN 61000 3 2 Certified with the safety standards Directive 2006 95 EC EN 61010 1 2001 EN 61010 1 Corr 1 2003 EN 61010 1 Corr 1 2003 EN 61010 2 101 2003 EMC EMI 7 8 CERTIFICATE OF CONFORMITY DMT A S Skejbyparken 152 8200 Aarhus N Denmark hereby declares its responsibility that the following product Confocal Wire Myograph System Model 120CW is covered by this certificate and marked with CE label conforms with the following standards EN 61010 1 2001 EN61010 1 Corr 1 2003 EN 61010 1 Corr 1 2003 EN 61010 2 101 2003 EN 61326 2 6 2005 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements Safety requirements for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD medical equipment Electrical equipment for measurement cont
16. ce using the grey 25 pin connection cable The end of the cable with the temperature probe is placed into the myograph chamber to monitor actual temperature 2 Myo Interface PC connection Data acquisition is possible either by connecting the Myo Interface directly to a PC or through a PowerLab data acquisition and analysis system optional l Direct PC connection Connect the Myo Interface to one of the COM ports on the PC using the serial cable delivered with the Confocal wire myograph 120CW system Il PowerLab optional Connect the Myo Interface to the PowerLab unit using BNC cables Connect Rec 1 on the Myo Interface to Input 1 on the PowerLab Rec 2 to Input 2 and so forth Connect the PowerLab unit to one of the USB ports on the PC using the USB cable delivered with the PowerLab system CHAPTER2 13 3 Oxygen supply Connect the small pipes on the myograph chamber cover to an adjustable oxygen supply using small silicone tubing inter nal diameter 1 5 mm 4 Suction connection Connect the large pipe on the myograph chamber cover to a vacuum pump via a suction bottle and the vacuum valve as illustrated in figure 2 2 below The internal diameter of rubber tubes for connection to the pipes on the chamber cover is 2 mm An electronic vacuum valve is available from DMT 5 Electrode optional Connect the pH electrode to the pH port on the backside of the Myo Interface and install the software flash update as de scribed in t
17. cium deposits and other salt build up Use a swab stick to mechanically clean all chamber surfaces 2 Remove the acetic acid and wash the myograph chamber and jaws several times with double distilled water If any kind of hydrophobic reagent have been used which might be difficult to remove using step 1 and 2 then try incubating the chamber and jaws with 96 ethanol or a weak detergent solution 4 To remove more resistant or toxic chemicals incubate the myograph chamber and glass cannulas with 1M for up to 1 hour In exceptional cases incubate the chamber and supports with a up to 3M HNO solution for about 15 minutes 5 Wash the myograph chamber and supports several times with double distilled water IMPORTANT NOTES BE VERY CAREFUL USING STEP 3 AND 4 REPEATEDLY AS STRONG REAGENTS CAN CAUSE EXTREME DAMAGE TO THE MYOGRAPH UNIT BE VERY CAREFUL NOT TO EXERT ANY FORCE ON THE MYOGRAPH JAWS DURING THE CLEANING PROCEDURE AFTER CLEANING ALWAYS CHECK THAT THE GREASING AROUND THE TRANSDUCER PIN IS SUFFICIENT TO KEEP OUT THE BUFFER SOLUTION FROM THE TRANSDUCER COMPARTMENT CHAPTER4 23 In cases of red or brown discolorations appearing on the chamber sides the following cleaning procedure will work in most cases 1 Incubate the myograph chamber for 30 minutes with 20yl of a 2 mM T 1210 Tetrakis 2 pyridylmethyl ethylenediamine solution dissolved in double distilled water 2 Use swabestick to mechanically clean a
18. e General navigation The following controls are used to display the various menus choose varying menu options and change values F1 Change to the next menu F2 F4 Have varying functions depending on the current active menu Info Push this key in any menu to display all specific associated F key functions Numeric keys Enter data or setting values Enter Confirm entered numeric values or change between available choices Esc Delete incorrectly entered numeric values In some submenus used to return to main menu Y Scroll up and down through the display as only four lines are capable of being displayed at a time Being in the top line of a submenu use to change to the previous main menu The active line in the menu is indicated by a gt symbol Main menu The Main menu displays the current values from the force transducer the actual temperature probe reading and heating setting in C the heating status as well as the pH probe reading optional Temperature To change the temperature setting move the Set Temp line to the top of the display the gt symbol is displayed in that line Use the numeric keys to enter a new temperature setting and press Enter to confirm Heating To turn on the myograph heating move the Heat is line to the top of the display and use the Enter key to switch between on and off Press the Info key and the display shows the F key options associated with the Main menu Choose one of
19. een panels indicate the menu appearance options when the optional pH meter is installed 16 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL Setup menu Calibrate Displays the signal to be calibrated either force or pH Valve dly The time in seconds that the electronic valve optional to the system remains open when activated Contrast The actual contrast setting in the display Baudrate Data transmission rate from the Myo Interface to a PC via the RS 232 Option The option line allows access to submenus using specific access codes Use the numeric keys to change the valve delay or display contrast settings and press Enter to confirm Press the Info key and the display shows the F key options available for the Setup menu Press Info again to return to the Setup menu or one of the F keys to proceed with one of these options F1 Press F1 to proceed to the Main menu FA Calibrate Force or pH Having chosen in the Setup menu which signal to calibrate press F4 to initiate the calibration process Press Esc to return to the Setup menu if you do not wish to calibrate Force calibration This procedure is described in detail in Chapter 4 pH calibration optional Having chosen to calibrate the pH settings press F4 to initiate the calibration In the Calibrate pH Offset display the first line shows the value of the offset buffer first buffer solution which is always 7 00 The second line shows the temperature of the buffer solutio
20. f the myograph chamber to remove any remaining old grease 3 Carefully apply a small amount of high vacuum grease around the edge using a wood stick or similar equipment Take a new window glass using forceps and gently push it into place Before starting a new experiment make sure that the complete edge is sealed up with high vacuum grease to keep the myograph window waterproof 4 5 Myograph maintenance The Confocal Wire Myograph System 120CW is a very delicate and sophisticated piece of research equipment In order to keep it working at its best DMT recommend that the following sections are read carefully and the instructions are followed at all times 4 5 1 Myograph chamber pipes To prevent the pipes from being blocked by buffer salt deposits after an experiment use the chamber cover to remove the clean ing solutions used and described later in this Chapter Afterwards remove the cover from the myograph chamber and turn on the vacuum pump for about 10 seconds Wait to turn off the oxygen supply until turning off the vacuum pump Wipe off any buffer remaining on the outside of the pipes using a piece of paper 4 5 2 Force transducer The force transducer is the most delicate and fragile component of the myograph system Therefore careful handling is neces sary One of the jaws is connected to the transducer pin To prevent the buffer from running into the transducer house the hole is filled with high vacuum grease As part of the daily
21. fe care use and should never be used for these purposes nor for the prevention diagnosis curing treatment or alleviation of disease injury or handicap Do not open the unit the internal electronics pose a risk of electric shock Do not use this apparatus near water To reduce the risk of fire or electric shock do not expose this apparatus to rain or moisture Objects filled with liquids should not be placed on the apparatus Do not block any ventilation openings Install in accordance with the manufacturer s instructions Do not install near any heat sources such as radiators heat registers stoves or other apparatus that produce heat Only use attachments and accessories specified by the manufacturer Unplug this apparatus during lightning storms or when unused for long periods of time This apparatus must be grounded Use a three wire grounding type cord similar to the one supplied with the product Do not defeat the safety purpose of the polarized or grounding type plug A polarized plug has two flat blades one being wider than the other A grounding type plug has two blades and a third round grounding pin The wide blade or the third prong is provided for your safety If the provided plug does not fit into your outlet consult an electrician for replacement of the obsolete outlet Be advised that different operating voltages require the use of different types of line cord and attachment plugs Check the voltage in your area
22. he provided procedure Perform a pH calibration as described in chapter 3 pee eene Inlet for adding drugs Suction connection Oxygen supply Spare connection for chamber superfusion CEE Figure 2 2 Chamber cover 2 3 The first force transducer calibration Prior to the shipment of the Confocal Wire Myograph System has gone through two days of continuous testing including a force transducer calibration However in order to ensure that the myograph is operating at an optimum DMT recommends that a new force transducer calibration is performed before the Confocal Wire Myograph System is used The force transducer calibration procedures are described in detail in chapter 4 14 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL CHAPTER 3 THE MYO INTERFACE 3 1 Turning on the Myo Interface When the 120CW Myo Interface is switched on the display shows that the system is starting up and initializing after which the start up message depicted the right is shown MODEL 120CW VERSION 3 30 28 02 2008 The display automatically shows the Main menu after several seconds Otherwise press F1 to proceed to the Main menu When the start up message is active the A and Y keys can be used to adjust the display contrast setting 3 2 Menus and submenus The layout and relationship between the various menus and associated submenus in the Myo Interface are depicted in the sche matic figure 3 1 on next pag
23. ibration procedure The section contains a complete step by step description of how to weight calibrate the force transducer L Fill the myograph chamber with double distilled water and move the jaws apart Mount a 40 um stainless steel wire on the jaws connected to the force transducers Place the calibration bridge balance and weight randomly on the myograph unit allowing it to be warmed up together with the myograph unit Turn on the heating in the Main menu on the Myo Interface After approximately 20 30 minutes the whole system will have reached the target temperature normally 37 C Place the warmed calibration bridge and balance on the myograph unit as illustrated in figure 4 3 below NOTE THE WEIGHT SHOULD NOT BE PLACED ON THE BALANCE YET Make sure that the tip of the transducer arm on the balance is placed in the gap between the wire and the jaw as illustrated in figure 4 4 on next page Carefully move the calibration bridge until the tip of the transducer arm is placed freely in the gap which means it does not touch either the wire or the jaw Go to the Setup menu on the Myo Interface and choose to calibrate the myograph force transducer Press F4 to start calibra tion Figure 4 3 Weight calibration setup showing placement of the calibration bridge and balance with weight in place 20 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL 6 Figure 4 4 Illustration of how to fit the balance between
24. ing into the transducer compartment due to insufficient greasing of the transducer pinhole This section describes how to check the force transducer for any kind of damage 4 3 1 Simple force transducer check L If the force reading on the Myo Interface appears unstable then first check that the Myo Interface and the Confocal Myo graph Unit are properly connected through the 25 pin grey cable If the force reading still appears unstable then perform a new weight calibration of the force transducer as described earlier in this chapter During the weight calibration monitor the relative force reading values in the Calibration menu on the Myo Interface If the value is O or above 6500 then the force transducer is broken and needs to be changed If the reading is between 1 499 or 3001 6250 then contact Danish Myo Technology for further instructions CHAPTER4 21 4 4 Changing myograph window glass The glass in the myograph chamber window is fixed in place and kept waterproof by a thin layer of high vacuum grease on the circular edge between the glass and the myograph chamber base Daily use of the myograph objectives that require water or other immersion solvents will mean that the glass needs to be replaced daily prior to mounting The following procedure describes how to change the myograph window glass 1 Carefully loosen the glass from the myograph chamber edge using small forceps or similar tool 2 Clean the edge at the bottom o
25. la B vb bd OL www dmt dk salesedmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554 www dmt asia com sales dmt asia com support dmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China Tel TES O 21 A25 1S Fax 86 0 21 5877 0063 www dmt asia com sales dmt asia com Support dmt asia com DMT USA Inc 201 East Liberty Street Suite 6 Ann Arbor MI 48104 USA Tel 1 770 612 8014 1 678 302 7013 www dmt usa com sales dmt usa com support dmt usa com
26. ll the affected surfaces during the last 15 minutes of the incubation period 3 Wash the myograph chamber several times with double distilled water 4 Incubate the myograph chamber with 96 ethanol for 10 minutes while continuing the mechanical cleaning with a swab stick 5 Remove the ethanol solution and wash a few times with double distilled water Incubate the myograph chamber with an 896 acetic acid solution for 10 minutes and continue the mechanical cleaning with a swab stick 6 Wash the myograph chamber several times with double distilled water IMPORTANT NOTES IN EXCEPTIONAL CASES IT MAY BE NECESSARY TO REMOVE THE SUPPORTS FOR INDIVIDUAL CLEANING TO MAKE SURE THAT ALL SURFACES ARE CLEAN Figure 4 8 The top part of the myograph can be tilted for easier access when cleaning 24 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL APPENDIX 1 FUSE REPLACEMENT The main fuse of the myograph system is placed inside the power inlet on the Myo Interface If the fuse blows it is easily changed using the following procedure When a fuse blows and needs to be changed it is imperative that the replacement fuse is equal to the one blown The 120CW system uses T1 6A 250 V 6 3 x 32 mm DMT recommends that both fuses in the fuse block are changed at the same time as it can be difficult to determine which fuse is blown To replace the fuses 1 Usea small screwdriver to open the voltage selector block 2 Remove the red fuse
27. maintenance it is very important to inspect the greasing of the transducer hole before starting any experi ment Insufficient greasing causes damage and malfunction of the force transducer IMPORTANT DMT RECOMMENDS THAT THE HIGH VACUUM GREASE SEALING THE TRANSDUCER PINHOLE IS CHECKED AND SEALED AT LEAST ONCE A WEEK ESPECIALLY IF THE MYOGRAPH SYSTEM IS USED FREQUENTLY DMT TAKES NO RESPONSIBILITIES FOR THE USE OF ANY OTHER KINDS OF HIGH VACUUM GREASE THAN THE ONE TO BE PURCHASED FROM DMT DMT TAKES NO RESPONSIBILITIES FOR ANY KIND OF DAMAGE APPLIED TO THE FORCE TRANSDUCER Figure 4 6 Transducer pin hole to be sealed up with high vacuum grease 22 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL 4 5 3 Linear slides Check the linear slides underneath the black cover for grease at least once a week In case of insufficient lubrication grease the slides with the original enclosed grease for linear slides at the places marked by the arrows in figure 4 7 below Figure 4 7 Greasing points on the linear slides NOTE only use the grease marked with Grease for linear slides 4 5 4 Myograph cleaning DMT STRONGLY RECOMMENDS THAT THE MYOGRAPH CHAMBER AND SURROUNDINGS BE CLEANED AFTER EACH EXPERI MENT After an experiment use the following procedure to clean the myograph chamber and supports 1 Fill the myograph chamber to the edge with an 8 acetic acid solution and allow it to stand for a few minutes to dissolve cal
28. n The temperature is an important parameter in the calibration formula and is obtained automatically by placing the myograph temperature probe in the buffer solution The third line shows the output from the pH probe as raw data from the A D converter Place the pH meter electrode and temperature probe in the offset buffer solution and turn on stirring When the relative pH output in the bottom line is stable press F3 to proceed The first line now displays the value of the slope buffer second buffer solution which is always 4 00 Place the pH and temperature probes in the slope buffer solution and turn on stirring When the relative pH output in the bottom line is stable press F3 to proceed The pH calibration is now finished The parameters are stored in the internal memory of the Myo Interface Press F3 to return to the Setup menu CHAPTER 3 17 CHAPTER 4 THE CONFOCAL WIRE MYOGRAPH UNIT Chapter 4 contains a complete explanation of how to adjust calibrate and maintain the Confocal wire myograph unit to ensure the equipment is always performing to its highest standard 4 1 Adjustment of supports A successful mounting of any kind of tubular tissue segment in the confocal wire myograph is very dependent on perfectly matching supports The supports are matched prior to the shipment but daily use of the myograph system and greasing of the transducer pinhole will over time create a need for an adjustment of the supports Adjustment of the
29. rol and laboratory use EMC requirements Part 2 6 Particular requirements In vitro diagnos tic IVD medical equipment With reference to regulations in the following directives 2006 95 EC 89 336 EEC CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL ABOUT THIS MANUAL This manual contains a complete list of procedures describing how to install maintain and using the Confocal Wire Myograph System model 120CW Chapter 1 provides an overview of the construction and basic features of the Myo Interface and the Confocal Wire Myograph unit Chapter 2 describe step by step how to set up a complete Confocal Wire Myograph System including connection to data acquisi tion Chapter 3 is a complete manual to the Myo Interface The chapter describes in detail the construction of the menu system and how to all the features of the Confocal Wire Myograph System Chapter 4 contains procedures describing general as well as daily maintenance of the myograph unit e g adjustment of sup ports weight calibration of the force transducer and cleaning instructions Appendix contain additional information such as fuse replacement and system specifications ABOUTTHIS 9 MANUAL UNPACKING THE MYOGRAPH SYSTEM Please take a few minutes to carefully inspect your new Confocal Wire Myograph System for damage which may have occurred during handling and shipping If you suspect any kind of damage please contact us immediately and we will
30. wire myographs 10 CONFOCAL WIRE MYOGRAPH SYSTEM MODEL 120CW USER MANUAL CHAPTER 1 SYSTEM OVERVIEW 1 1 Confocal wire myograph unit e 25 serial cable for communication with Myo Interface Vertical micrometer screw Horizontal micrometer Allen screws for fine alignment of the myograph jaws 2 Myograph jaw connected to horizontal Myograph jaw connected eese p micrometer force transducer N Force transducer s Il1 Window in the base of the chamber for imaging Figure 1 1 The confocal wire myograph unit with close up detail of the chamber CHAPTER 1 11 1 2 Myo Interface front panel Analog recorder output Connection to external data acquisition system optional e Up and down arrow keys Scroll and down in the menus Myo Interface display e I Serle 2x s F keys Have different but specific func Numeric keys tions dependent of the active main menu Enter data or setting values INFO key Push this key in any menu to i ifi Enter key Confirm entered numeric values or display all specific F key functions change between available choices P ns e Esc key Delete incorrectly entered numeric values In some submenus used to return to main menu Figure 1 2 Myo Interface front panel 1 3 Myo Interface rear panel 115 230 V 50 60 Hz automatic voltage selector
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