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1. LSS Calibrating with the electrode outside the flow cell When calibrating the electrode out of the flow cell and changing from one buffer to another rinse the electrode tip with distilled water and dab it carefully with a soft tissue to absorb the remaining water Do NOT wipe the electrode as this may charge it and give unstable readings The steps below describe the procedure used with the electrode removed from the flow cell Note The user interface of the monitor must be unlocked if connected to a UNICORN control system 1 Remove the pH electrode from the flow cell and immerse the electrode in the first standard buffer solution normally pH 7 0 Set Parameters 2 From main menu 1 turn the dial four steps clockwise to enter menu Set Parameters Press OK Set Parameters 3 Select Set Parameters pH Press OK Calibrate pH 4 Select menu Calibrate pH Current calibration values are displayed 7 00 12 00 buffer 1 buffer 2 Buffer 1 fixed lower calibrated pH value Range 0 00 14 00 Buffer 2 fixed higher calibrated pH value Range 0 00 14 00 Note The pH values for buffer 1 and 2 must differ by at least 1 pH unit Calibr pH Buffer 1 5 Press OK to access the settings menu The order of calibration buffer 1 or buffer 2 is optional Press OK to start with buffer 1 or turn the dial to start with buffer 2 In this example we start with buffer 1 Calibr pH Buffer 1 6 This text disappe
2. See next page Setup Setup for sub menus Setup Language Set Clock Setup Unit Number Set Timer z 18 34 52 00 00 00 Setup Display Angle Set Alarm 00 32 21 Alarm Timer 12 30 52 Alarm Timer off Buzzer 08 23 34 48 Monitor UPC 900 User Manual 18 1125 55 Edition AG Reference information 3 B 5 Menu overview continued Continued from previous page Setup Setup Adjust Temp Temp sub menus 7 Warning This will Setup Adjust Temp Setup Show Temp on Setup Show Temp on on off Setup Cond Temp Comp 0 0 0 0 Setup Cond Ref Temp 25 0 25 0 Cond sub menus Warning This will Setup Cond Temp Comp change cell calib Setup Adjust Cond Setup Cond Ref Temp gramm 83 53mS cm 86 6 Setup Adjust Cond a2 53 m lt lem Warning This will Setup Adj Cell Const change cell calib Setup Adj Cell Const a cond 83 56mS cm 86 6 Setup Show Cond on on off pH sub menus Setup pH Temp Comp Setup pH Temp Comp loff on off of Setup Show pH Setup Show pH on on on off UV sub menus Set Averaging 1 35 0 64 Set Averaging Setup L
3. AS Inserting the pH electrode Note Handle the pH electrode with care CAUTION The tip of the pH electrode consists of a thin glass membrane Protect it from breakage contamination and drying out or the electrode will be destroyed Always store the electrode with the end cover filled with a 1 1 mixture of pH 4 buffer and 2 M KNO Do NOT store in water only 1 Unpack the pH electrode Ensure that it is not broken or dry 2 Before using the electrode remove the electrode end cover and immerse the glass bulb in buffer for 30 minutes To module pH probe h 3 Remove the dummy electrode from the flow cell and store it in the flow socket J cell holder 4 Carefully insert the electrode in the flow cell Tighten the nut by hand to End secure the electrode Note Ifthe flow cell is full of liquid it is not possible to insert the electrode If so loosen the inlet connection while inserting the electrode to allow the liquid to run out from the flow cell Remember to re tighten the connector Flow cell Note Ifthe electrode is not fully inserted the system will leak and a dead Flow cell holder volume will occur in the holder holder 5 Connect the pH electrode cable to the socket pH Probe on the rear of the module Monitor UPC 900 User Manual 18 1125 55 Edition AG 7 Installation 2 6 Connecting electrical signal cables The sockets for electrical signals are located on the re
4. The electrode glass membrane may be cracked If so replace the electrode Clean the pH electrode according to section 4 8 and recalibrate If the problem remains replace the pH electrode Check the electrode glass membrane If it is contaminated clean the electrode following the instructions in section 4 8 Cleaning the pH electrode If the membrane has dried out the electrode may be restored by soaking it in buffer overnight Clogged liquid junction Refer to section 4 8 Cleaning the pH electrode Check that the electrode cable is connected properly to the rear of the module Check that the pump operates correctly Check that the electrode is correctly inserted in the flow cell and if necessary hand tighten the nut If air in the flow cell is suspected tap the flow cell carefully or tilt it to remove the air Alternatively flush the flow cell with buffer at 20 ml min for 1 2 min Use a flow restrictor after the pH electrode Check that the pH electrode is not broken Check that the pH electrode is calibrated Check the slope see section 3 10 Calibrating the pH electrode If it is outside the range 80 105 or if the asymmetry potential deviates more than 60 mV from O mV clean the pH electrode Recalibrate If the problem persists replace the pH electrode Clean the pH electrode if required see section 4 8 Cleaning the pH electrode Compare the response of the pH electrode with that of another pH electrode If the
5. The length should be 170 mm when using the 5 mm flow cell and 230 mm when using the 2 mm flow cell 5 Connect the other end of the tubing to the conductivity flow cell or to another appropriate unit 2 4 Installing the conductivity flow cell 1 Place the conductivity cell in its cell holder or in a suitable location as close to the optical unit column as possible The cell can be placed up to 1 5 m from the monitor housing Secure the cell with the clamp Note When the conductivity flow cell is used in conjunction with the pH electrode place the conductivity flow cell and select its flow direction so that the screw head end of the flow cell faces the pH flow cell 2 Connect the conductivity cell to the socket Conductivity Flow Cell on the rear panel of the module 3 Connect the tubing with fingertight connectors Monitor UPC 900 User Manual 18 1125 55 Edition AG Installation 2 5 Installing the pH flow cell and electrode Mounting the flow cell holder In AKTA design systems the pH electrode is optional 1 Hook the flow cell holder on the right hand side of the housing Secure it with the slide clamp If the flow cell holder is not used the flow cell must still be installed at an angle of 30 from the vertical with the outlet placed higher than the inlet to prevent air bubbles being trapped in the cell The flow direction is marked on the flow cell Flow direction 2 Connect the tubing with fingertight connectors
6. AU Cond Tc pH Lamp on Autozero UV Eventmark pH 12 50 22 4 Set Parameters Check Setup Alarm Timer 00 22 24 Lamp on Lamp on on off Operation 3 3 Main menu overview Main menu 1 This menu is accessed from all positions by pressing ESC Lamp control This menu is accessed by turning the dial one step counter clockwise from main menu 1 It is used to manually switch the lamp on off Autozero Used to zero the UV signal from the monitor Eventmark Used to set eventmarks on the UV curve Main menu 2 Used to display pH temperature in the conductivity flow cell and conductivity in mS cm as well as in percentage of full range Parameter menus Used to set measurement parameters for Cond pH and UV Check menus Checking internal operating values See Reference information section B 1 Setup menus Setting up language unit number etc See Reference information section B 2 Alarm Timer menus Setting different timer options See Reference information section B 3 3 4 Controlling the UV lamp The Lamp menu is accessed by turning the dial one step counter clockwise from main menu 1 and then pressing OK We recommend the lamp be switched off to conserve lamp operating time when no measurement is being made A warm up time of 60 minutes is required to achieve full specifications However in most cases a warm up time
7. Reference information Conductivity measurement Conductivity range 1 uS cm to 999 9 mS cm Reproducibility short term Max 1 or 5 uS cm whichever is greater long term Max 3 or 15 uS cm whichever is greater Noise Max 0 5 of full scale calibrated range Response time lt 3 s 0 95 of step Temperature sensor accuracy 2 0 C drift 0 5 C per 10h Flow rate sensitivity 1 within 0 100 ml min Conductivity flow cell Flow rate 0 100 ml min Max pressure 5 MPa 50 bar 725 psi Generated backpressure Max 0 01 MPa at 100 ml min pH measurement pH range 0 to 14 spec valid between 2 and 12 Accuracy temperature compensated 0 1 pH within 4 to 40 C not temperature compensated 0 2 pH within 15 to 25 C 0 5 pH within 4 to 15 C and 25 to 40 C Response time lt 10 s 0 95 of step Long term stability Dev max 0 02 pH h measured at pH 4 0 Flow rate sensitivity Dev max 0 1 pH units pH flow cell Flow rate 0 1 100 ml min Max pressure 0 5 MPa 5 bar 72 psi Generated back pressure Max 0 02 MPa 0 2 bar 2 9 psi at 100 ml min 52 Monitor UPC 900 User Manual 18 1125 55 Edition AG C 2 Physical data Control Degree of protection housing Power requirements Power consumption Functions UV lamp cable length Optical unit cable length pH electrode cable length Cond cell cable length Tubing connections Analogue outputs Display Dimensions H x W x D Weight Wetted
8. 01234567 YM 012345 UPC 900 V1 00 Date of Maintenance Buzzer Test Setup Temp Cond pH UV Setup Temp Cond pH UV Setup Adjust Temp 22 3 C Warning This will change temp calib Setup Adjust Temp 22 3 C 223 40 ED Reference information 3 The service telephone number is displayed press OK 4 The service contract number is displayed press OK 5 The module serial number is displayed press OK 6 The module name and software version are displayed press OK 7 The date of the last service is displayed press OK 8 Atest of the module s buzzer is performed press OK B 2 Setup menus B 2 1 Selecting setup sub menus There are setup sub menus for temperature conductivity pH and UV absorbance 1 Select menu Setup and press OK 2 Select sub menus Temp Cond pH or UV with the dial and press OK B 2 2 Setup adjust temperature Calibration of the temperature sensor in the conductivity flow cell is only necessary if the monitor is used in high accuracy measurement or if the conductivity flow cell is replaced 1 Place the flow cell together with a precision thermometer inside a box or empty beaker to ensure that they are not exposed to draught Leave them for 15 minutes to let the temperature stabilise 2 Read the temperature on the thermometer 3 Select sub menu Setup Temp and press OK 4 Select sub menu Setup Adjust Temp a
9. Filter wheel cpl 1 A 18 0647 01 Conductivity flow cell complete 1 A 18 1111 05 pH electrode round tip 1 C 18 1111 26 pH flow cell round tip including 1 A 18 1112 92 dummy electrode Dummy electrode round tip 1 A 18 1111 03 pH electrode complete round tip incl 1 C 18 1134 84 pH electrode flow cell and holder Flow cell holder UPC 900 1 A 18 3055 87 Clamp Conductivity flow cell 1 A 18 1111 14 PEEK tubing i d 0 50 mm o d 1 16 G 2m A 18 1113 68 PEEK tubing i d 0 75 mm o d 1 16 2m A 18 1112 53 Union 1 16 female M6 male PEEK 6 A 18 1112 57 Fingertight connector 1 16 or PEEK tubing o d 1 16 10 A 18 1112 55 Union M6 female 1 16 male PEEK 8 A 18 1112 58 Union 1 16 male 1 16 male or 1 16 o d tubing PEEK 10 A 18 1120 92 Monitor UPC 900 User Manual 18 1125 55 Edition AG 55 D Reference information 56 Tubing cutter U wrench M6 U wrench 1 4 Signal cable 6 pin mini DIN open Chart Recorder REC 111 1 channel Chart Recorder REC 112 2 channel al A 18 1112 46 ol A 19 7481 01 1 A 18 1112 45 al A 18 1110 64 1 A 18 1132 32 1 A 18 1132 33 A accessories C consumables Monitor UPC 900 User Manual 18 1125 55 Edition AG Short instructions Short instructions Calibrating Lamp AU Cond Tc pH 0 00002 015 0 12 50 Autozero UV Eventmark Calibrate pH Setup Adjust Cond The following short instructions
10. 436 and 546 nm through filter change e Two alternative flow cells with pathlengths of 2 mm and 5 mm e Fast response e High accuracy and reproducibility e Flow cells with low dead volume e Accurate and reliable monitoring through self test and self calibration e Flow cells can be connected close together minimising band broadening and time delay between detectors QO The monitor and the flow cells are separate units All flow cells are connected to the rear of Monitor UPC 900 Monitor UPC 900 User Manual 18 1125 55 Edition AG 1 Introduction Bee DDD 12 Safety IMPORTANT Monitor UPC 900 is intended for laboratory use only not for clinical or in vitro use or for diagnostic purposes The module is designed for indoor use only Do not use in a dusty atmosphere or close to spraying water Operate in accordance with local safety instructions WARNING The module must be connected to a grounded mains socket WARNING Always disconnect the power supply before attempting to replace any item on the module during maintenance WARNING The module must not be opened by the user It contains high voltage circuits that can deliver a lethal electric shock WARNING When lamp power is on the lamp socket carries a dangerous voltage Do not connect disconnect with the monitor switched on WARNING The module uses high intensity ultra violet light Do not remove the UV lamp w
11. Monitor UPC 900 User Manual 18 1125 55 Edition AG 47 ED Reference information B 5 Menu overview Lamp on Lamp AU Cond Tc pH 0 00002 015 0 12 50 I Autozero UV 1 Calibrated Electrode Eventmark Calibr pH Buffer 2 10 00 Set Cond Full Scale en E E Card eA eE Calibr pH Buffer 2 10 00 Set Cond Zero Level Calibr pH Buffer 2 Set parameters Set Cond Zero Level Calibr pH Buffer 1 7 00 Set Cond Analog Out Set Cond Analog Out Calibr pH Buffer 1 7 00 Calibrate pH Calibr pH Buffer 1 Set parameters Set pH Analogue Out Set pH Analogue Out Set UV Analogue Out Set UV Analogue Out Set pH Zero Level Set UV Range Set pH Zero Level Set UV Range Set pH Full Scale Set UV Zero Level Set pH Full Scale Set UV Zero Level Check Check Autozero AZ 0 0356 AU Check Lamp Run Time Lelephone Service Contract Number Check Lamp Intensity 0 22456722001 Serial Number yee MAA N19740 UPC 900 Check Service Mode V Date of Maintenance Check Recorder Buzzer Test
12. are intended as a guide to users who are fully familiar with the safety precautions and operating instructions described in this manual These short instructions assume that the module is installed according to the installation instructions Select 1 Switch on the module with the mains switch on the rear panel 2 Switch the lamp on off 3 The main operating menu displays measured parameters 4 Autozero the module by pressing OK 5 Set Eventmark by pressing OK 6 Calibrate the pH electrode before use and or daily by using 2 buffers with known pH values 7 The conductivity flow cell does not normally need to be calibrated 83 53m 83 56mS cm Setup Adj Cell Const 86 6 Set parameters 8 To connect a chart recorder set the Analogue output scaling 0 100 for measured parameters 9 Always store the pH electrode in a 1 1 mixture of pH 4 buffer and 2 M KNO when not in use For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com protein purification imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company AKTA Drop Design and UNICORN are trademarks of GE Healthcare companies Purification and preparation of fusion proteins and affinity peptides comprising at least two adjacent histidine residues may require a licens
13. conductivity cell Clean the flow cell and recalibrate 5 Ifthe problem remains change the conductivity cell ERROR key OK 1 The key was pressed during self test or is faulty Switch off the module 3 Switch on the module ERROR key Esc ERROR key OK Esc BAER nine na 1 Switch off the module 2 Check all connections 3 Switch on the module ERROR number 109 113 ERROR number 120 121 ERROR number 106 108 1 Switch off the module 2 Check all UniNet 1 and UniNet 2 connections 3 Switch on the module ERROR number 118 1 Switch off the module Check all connections 3 Switch on the module Exc x y in ab c Exc DIV O in ab c c instr in ab c m x Exc address in ab c x Monitor UPC 900 User Manual 18 1125 55 Edition AG 33 Trouble shooting 34 Monitor UPC 900 User Manual 18 1125 55 Edition AG Reference information A Description A 1 Module Monitor UPC 900 is an on line monitor for measurement of UV absorption pH optional in KTA design and conductivity The monitor can work with standard glass pH electrodes with a built in liquid filled reference electrode and a BNC connector LDIECDBDECDECDICDICDIEDEDEDEDED 9 eee Optical Anclque Conductivity O O ie ande Unit outoav Uninet 1 O7 D6D00 Conductivity measurement has a dynamic range from 1 uS to 999 9 mS cm and is suitable for a
14. current filter constant is shown Valid values are 0 02 0 04 0 08 0 16 0 32 0 64 1 3 2 6 5 and 10 seconds 3 Change the setting if desired and press OK Monitor UPC 900 User Manual 18 1125 55 Edition AG Setup Temp Cond pH UV Setup Lamp Run Time Hg 2000h Setup Lamp Run Time Hg 2000h 000 Setup Temp Cond pH UV Setup Show UV on Setup Show UV on on off Setup Language GB GB DE FI Reference information 3 B 2 12 Setup lamp run time When the UV lamp is replaced the Lamp Run Time counter should be reset 1 Select sub menu Setup UV and press OK 2 Select sub menu Setup Lamp Run Time and press OK 3 Set the Lamp Run Time counter to zero with the dial Press OK to acknowledge B 2 13 Setup show UV Normally UV absorbance is displayed in main menu 1 If not required the UV absorbance display can be set to off 1 Select sub menu Setup UV and press OK 2 Select sub menu Setup show UV and press OK The current status for showing UV is displayed If on is shown the current UV value is displayed in main menu 1 If off is shown no UV value is displayed in main menu 1 3 Change the setting if desired and press OK B 2 14 Setup language The language used on the display can be changed 1 Select main menu Setup and press OK 2 Select sub menu Setup Language and press OK 3 Select the desired language GB English D German
15. default Adjust the compensation factor setting as necessary and press OK B 2 5 Setup conductivity reference temperature 1 Select sub menu Setup Cond and press OK 2 Select sub menu Setup Cond Ref Temp and press OK The current reference temperature value is shown 25 0 C is the default setting The range is 0 00 99 9 C 3 The current reference temperature value is displayed as default Adjust the reference temperature value setting as necessary and press OK B 2 6 Setup adjust conductivity Normally it is not necessary to adjust the cell constant as the flow cell is pre calibrated on delivery Adjustment is only necessary when replacing the conductivity flow cell with a flow cell whose cell constant is unknown We recommend that the conductivity flow cell is recalibrated after cleaning When adjusting the cell constant from UNICORN select System Control System Calibrate and then select CondCalib Note The conductivity temperature compensation must not be used when adjusting the cell constant Set the Setup Cond Temp Comp to 0 see section B 2 4 The temperature sensor must be calibrated before adjusting the cell constant see section B 2 2 Monitor UPC 900 User Manual 18 1125 55 Edition AG 41 ED Reference information 1 Prepare a calibration solution of 1 00 M NaCl 58 44 g l Let the solution stand until it is at room temperature This is important for exact measurements 2 Fill the flow cell completely with the calibrati
16. electrodes in the flow cell may be contaminated and require cleaning To clean the flow cell 1 Pump 15 ml of 1 M NaOH at 1 ml min through the flow cell either by using a pump or a syringe 2 Leave it for 15 minutes 3 Rinse thoroughly with 50 ml distilled water Note If the flow cell is totally blocked the blockage can be removed using a needle or a wire with a diameter less than 0 8 mm 4 7 Changing the conductivity flow cell The conductivity flow cell can be changed when required Make sure the monitor is switched off before disconnecting connecting the cell from the rear of the monitor housing If the cell is replaced with a new flow cell the monitor must be calibrated with the new cell constant value written on the flow cell package See section B 2 in Reference information If the cell constant is not known it can be determined see also section B 2 in Reference information Monitor UPC 900 User Manual 18 1125 55 Edition AG 25 Maintenance 4 8 Cleaning the pH electrode Note The pH electrode has a limited lifetime and should be replaced every six months or when the response time is slow or the slope is out of range lt 80 WARNING HCI and NaOH are injurious to health Avoid spillage WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and
17. in the flow cell Check that the flow restrictor gives a back pressure of 0 2 MPa If there is a lot of air in the water degas the buffer before use Check the connections of the optical unit Clean the UV cell see sections 4 3 and 4 4 Locking nut in optical unit not properly tightened Turn the locking nut to the stop position Pr oo WM Check that there is no air in the eluents Degas if necessary Clean the system in accordance with the KTA design System Manual Clean the column in accordance with the column instructions Check that the mixer is functioning correctly and that the correct chamber volume is being used PWN PrP Monitor UPC 900 User Manual 18 1125 55 Edition AG 27 Trouble shooting SS Fault Action Low sensitivity 1 Aging lamp Check the lamp and replace if necessary 2 Wrong lamp position Check that the lamp position and the wavelength used filter position fit together 3 Dirty on line filter Clean or replace the filter Error in external 1 Check the chart recorder in accordance with its manual chart recorder Test the recorder function by selecting recorder test according to section B 1 in Reference Information pH measurement optional in AKTA design systems No response to pH changes Small response to pH changes Slow pH response or calibration impossible Incorrect unstable pH reading 28 1 Check that the electrode cable is connected properly to the rear of the module
18. maintenance of the system gt gt Use one of the following procedures to clean the electrode to improve the response e Salt deposits Dissolve the deposit by immersing the electrode first in 0 1 M HCI then in 0 1 M NaOH and again in 0 1 M HCI Each immersion is for a 5 minute period Rinse the electrode tip in distilled water e Oil or grease films Wash the electrode tip in a liquid detergent and water If the film is known to be soluble in a particular organic solvent wash with this solvent Rinse the electrode tip in distilled water e Protein deposits Dissolve the deposit by immersing the electrode in a 1 pepsin solution in 0 1 M HCI for five minutes followed by thorough rinsing with distilled water If these procedures fail to rejuvenate the electrode the problem is most likely a clogged liquid junction 1 Heat a 1 M KNO solution to 60 80 C 2 Place the electrode tip in the heated KNO solution 3 Allow the electrode to cool while immersed in the KNO solution before re testing If these steps fail to improve the electrode response replace the electrode 4 9 Changing the pH electrode See section 2 4 Installing the pH flow cell and electrode 4 10 Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information co
19. of 15 minutes is sufficient Switching the lamp on off 1 Switch the lamp on off by moving the cursor with the dial and then pressing OK Monitor UPC 900 User Manual 18 1125 55 Edition AG 13 Operation Ge d Tc pH gt Absorbance value Autozero UV Eventmark Set Parameters Set Parameters Set Cond Analog Out 14 3 5 Reading the UV absorbance value The main menu 1 shows the absorbance value with four digits for the chosen wavelength This menu is reached from any other menu by pressing ESC If the lamp is off Lamp Off is displayed instead 3 6 Autozeroing UV The autozero function in the UV part of the module sets the detected absorbance to zero when OK is pressed Using autozero is recommended at the start before the sample is injected 1 From main menu 1 turn the dial one step clockwise to select Autozero UV then press OK The normal absorbance value display is then shown In UNICORN Autozero is set with the instruction AutoZeroUV in System Control Manual Alarm amp Mon 3 7 Creating eventmarks Eventmarks can be set and displayed as spikes on the UV curve for example when the sample is injected The spikes are 10 of the full scale AU setting 1 From main menu 1 turn the dial two steps clockwise to select Eventmark then press OK A spike on the UV curve is created 3 8 Setting Cond pH and UV analogue outputs This settings menu is
20. the flow cell 2 Insert the flow cell into the detector housing from above Note The flow cell can only be placed in one correct position 3 Secure the flow cell by turning the locking nut until the stop position Note If the locking nut is not tightened sufficiently the monitor will function poorly e g drifting base line 4 Place the protective cover around the flow cell to protect the electronics inside the optical unit from liquid spillage Note Avoid spillage for prolonged monitor lifetime 5 To remove the flow cell reverse the procedure Note Ensure that the Hg lamp position and the filter is selected according to the wave length to be used This is described in the Changing lamp assembly section below Connecting the optical unit to the module 1 Place the optical unit in the holder or in a suitable location as close to the column as possible The optical unit can be placed up to 1 5 m from the monitor housing 2 Secure it by tightening the screw in the holder 3 Connect the lamp cable to the socket Lamp on the rear panel of the module 4 Connect the signal cable to the socket Optical unit on the rear panel of the module Monitor UPC 900 User Manual 18 1125 55 Edition AG Installation Changing lamp assembly WARNING The module uses high intensity ultra violet light Do not remove the UV lamp while the monitor is running Before changing a defective lamp ensure that the lamp cable is disconnected fro
21. 3 14 Storage and shut down 3 15 Restart after power failure Monitor UPC 900 User Manual 18 1125 55 Edition AG V Contents 4 Maintenance 4 1 Periodic maintenance 4 2 Cleaning and checking the module 43 Cleaning the UV flow cell in place e 4 4 Cleaning the UV flow cell off line e 45 _ Cleaning the conductivity flow cell in place 24 4 6 Cleaning the conductivity flow cell off line 24 4 7 Changing the conductivity flow cell 24 4 8 Cleaning the pH electrode 25 49 Changing the pH electrode 26 4 10 RECYCIINY dns 26 5 Trouble shooting 51 VH EN TO ennemies 52 REGY 5 3 Faults and actions 5 4 M TTOTIM SSAUES nent Reference information A Descriptions IEERIRIS mag 34 Al Module 34 A2 UV optical Unit nes 35 A 3 pH electrode 36 AAs Condeutiviiy cellsseskeanissesniisenieene 36 B Mende B 1 Check menus B 1 1 Checking autozero level 37 B 1 2 Checking lamp run time 37 B 1 3 Checking lamp intensity 37 B 1 4 CHECKING recorder 37 B 1 5 Checking service mode 37 VI Monitor UPC 900 User Manual 18 1125 55 Edition AG Contents B 2 Setup FMS IOS sesessscssszcssseassscsssescaznnassatseavsaassseonsaassasncdoansssottseactsee 38 B 2 1 Selecting setup sub menus 38 B 2 2 Setup adjust temperature 38 B 2 3 Setup show temperature 39 B 2 4 Setup conductivity temperature COMPENSATION creme 39 B 2 5 Setup conductivity referen
22. B 1 2 Checking lamp run time The lamp run time can be checked to determine the need for lamp replacement Run times for both Hg and Zn lamps are monitored Check Lamp Run Time 1 Select menu Check and press OK Hg 2300h Zn 340h 2 Select sub menu Check Lamp Run Time B 1 3 Checking lamp intensity The lamp intensity can be checked to determine the status of the lamp used hack Len Mens 1 Select menu Check and press OK 2 Select sub menu Check Lamp Intensity B 1 4 Checking recorder The function of a connected chart recorder can be tested Check Recorder 1 Select menu Check and press OK 2 Select sub menu Check Recorder and press OK 3 Start the test by selecting on and press OK Check Recorder Please wait The test will ramp the signal on each channel Signal to recorder up to 1 V and then decrease the signal in 10 steps back to 0 V The test is run continuously Compare the diagram of the chart recorder ov with the figure 4 Stop the test by pressing OK or ESC B 1 5 Checking service mode Service information relevant to the module can be checked Information may not be available in all menus 1 Select main menu Check and press OK Check Service Mode 2 Select sub menu Check Service Mode and press OK Monitor UPC 900 User Manual 18 1125 55 Edition AG 39 Telephone Service 012345678901 Contract Number 2345678901 oO Serial Number
23. F French E Spanish Italian Monitor UPC 900 User Manual 18 1125 55 Edition AG 45 ED Reference information B 2 15 Setup unit number The unit number is the identification the Monitor UPC 900 has on the UniNet bus It should correspond to the number set in UNICORN for the Monitor UPC 900 The number should be set to 0 if one UPC 900 is used If more than one UPC 900 monitor is used they must all have different numbers 1 Select main menu Setup and press OK Sawo Uni Number 2 Select sub menu Setup Unit Number and press OK 3 Select unit number 0 25 and press OK B 2 16 Setup display angle Sets the display angle to compensate for different viewing heights 1 Select main menu Setup and press OK Set Display Angle 2 Select sub menu Set Display Angle and press OK 3 Select viewing angle gt Up gt Mid or gt Down 46 Monitor UPC 900 User Manual 18 1125 55 Edition AG Alarm Timer 12 30 52 Set Alarm 12 32 21 0 00 00 00 Set Timer 18 34 52 00 00 00 Alarm Timer 12 35 16 Buzzer 00 33 00 00 00 29 13 08 45 I Alarm time I Alarm time Set Clock 12 26 53 12 36 53 Alarm Timer off Buzzer 18 34 52 Insert Access Code Reference information 3 B 3 Setting and using the alarm timer You can set the alarm function to either a fixed alarm time or use a count down timer You cannot set both an ala
24. GE Healthcare AKTA design Monitor UPC 900 User Manual KTA Important user information All users must read this entire manual to fully understand the safe use of AKTA monitor UPC 900 Workstation WARNING The WARNING sign highlights instructions that must be followed to avoid personal injury It is important not to proceed until all stated conditions are met and clearly understood WARNING Read the instruction to avoid hazardous conditions CAUTION The Caution sign highlights instructions that must be followed to avoid damage to the product or other equipment It is important not to proceed until all stated conditions are met and clearly understood Note The Note sign is used to indicate information important for trouble free and optimal use of the product CE Certifying This product meets the requirements of applicable CE directives A copy of the corresponding Declaration of Conformity is available on request The CE symbol and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Recycling This symbol indicates that the waste of electrical X and e
25. a slope of 95 102 and an asymmetry potential within 30 mV As the electrode ages the slope decreases and the asymmetry potential increases As a rule when an electrode has an asymmetry potential outside of 60 mV and a slope lower than 80 and no improvement can be achieved by cleaning it should be replaced An electrode is still usable at lower slopes and higher asymmetry potentials but the response will be slower and the accuracy diminished Calibrating with the electrode in the flow cell When calibrating with the electrode fitted in the flow cell in AKTA design follow the above procedure but let at least 30 35 ml with 2 ml mixer of standard buffer solution be pumped through the system to stabilize pH Leave the pump running while calibrating Switch to the second standard buffer solution and repeat the procedure For a description of calibration from UNICORN with the electrode fitted in the flow cell see Chapter 6 in the UNICORN User Manual Monitor UPC 900 User Manual 18 1125 55 Edition AG Operation mi 3 11 Filtering noise in the UV signal To filter the noise in the UV signal a moving average filter is used The averaging time is the time interval used for calculating the moving average of the absorbance signal A long averaging time will smooth out noise efficiently but it will also distort the peaks Peaks narrower than the minimum peak width value according to the table below may be dist
26. aling and pH scaling see section 3 8 Setting the Cond analogue output and section B 1 4 Checking recorder No text on the front 1 Check that the mains cable is connected and the power switch is display in ON position 30 Monitor UPC 900 User Manual 18 1125 55 Edition AG Message Trouble shooting 5 4 Error messages If the suggested actions do not correct the fault call GE Healthcare Action 34 Start up failed Retry Call service 35 WARNING wrong averaging time set 50 Electrical error Call for service 57 Electrical error Call for service 75 Electrical error Call for service 70 Lamp disconnected If not call service 71 WARNING low light intensity 72 Change lamp or call for service 76 Change lamp or call for service 73 WARNING Too much straylight leaks in 77 WARNING Autozero Out of range 1 Perform a new start up The preceding message may tell more about the cause 2 If not call service 1 Wrong value for averaging time set See section 3 11 on how to set the averaging time relative to peak width 1 Call for Service 1 Connect the lamp or call for service 1 Check connection between optical unit and monitor 2 Check that lamp and filter position correspond 3 Change lamp If warning persists call for service 1 If used in cold room additional warm up might be needed 2 If problem remains ch
27. amp Run Time Setup Lamp Run Time Hg 2a00bi Hg 2000h 3000 Setup Show UV lon Setup Show UV on on off EEE Monitor UPC 900 User Manual 18 1125 55 Edition AG 49 ED Reference information 50 Monitor UPC 900 User Manual 18 1125 55 Edition AG C Technical specifications Reference information The full specifications apply only after at least 1 hour warm up C 1 Operating data UV measurement Absorbance range Autozero range Baseline adjust Wavelengths Hg lamp fixed by changing filter Zn lamp Linearity 5 mm cell Static noise short term long term Static drift Flow sensitivity UV flow cell 2mm Flow rate Max pressure Back pressure Liquid temperature range Optical pathlength Cell volume UV flow cell 5 mm Flow rate Max pressure Back pressure Liquid temperature range Optical pathlength Cell volume Monitor UPC 900 User Manual 18 1125 55 Edition AG 0 01 5 AU full scale 0 2 2 0 AU Adjustable 0 100 of full scale 254 and 280 313 365 405 436 and 546 nm 214 nm lt 3 up to 2 AU at 254 nm lt 5 up to 1 AU at 280 nm 40x10 6 AU at 254 nm typically 6x10 6 AU at 254 nm 40x10 6 AU at 254 nm 100x10 6 AU h at 254 nm 2x10 4 AU min ml 0 100 ml min 4 MPa 40 bar 580 psi Max 0 05 MPa at 100 ml min 4 to 60 C 2mm 2 ul 30 ul detector volume 0 20 ml min 4 MPa 40 bar 580 psi Max 0 02 MPa at 20 ml min 4 to 60 C 5mm 6 ul 10 ul detector volume 51
28. ange the lamp 3 If problem remains call for service 1 Check that filter wheel cover is closed 2 Check that non transparent tubings are used at UV flow cell inlet and outlet 3 Check that optical unit is not exposed to direct sunlight If problem remains call service 1 Auto is not allowed from a level above 2 AU Check buffers 3 Clean UV cell Monitor UPC 900 User Manual 18 1125 55 Edition AG 31 Trouble shooting 83 WARNING temp_ca will be changed 84 WARNING cond_cal will be changed 85 WARNING condsca 0 100 lt 0 1mS oO 86 WARNING cond_cell bad not connected 87 WARNING pH probe bad not connected 88 Electrical error Call for service 89 Electrical error Call for service 90 ATTENTION set lt 0mV first 91 WARNING bad pH ad value 92 WARNING electrode slope lt 70 or gt 110 93 pH_cal failed check electrode 94 WARNING lt 1pH unit between cal_buff 1 amp 2 95 Temp cal failed check cond cell 97 WARNING pH scale 0 100 lt 1pH unit 32 Press OK to accept the change Press ESC to skip the change The difference between 0 and 100 must be at least 0 1 mS cm Increase the span between zero and full scale setting See section 3 8 Check that the conductivity cell is connected Recalibrate temperature If problem remains replace the conductivity cell Check t
29. ar panel Ca Me Ca Gs sms zum ns mi es ns 0 aus aus au aus aus aus ame am am am am Optical Ancogue Conductivity oO ni outos ni Flow Cell Unit it 0 1 UniNet 1 O7 0600 Connecting to chart recorder if used The external chart recorder outputs for UV pH and conductivity from the monitor are 0 1 V 1 Connect the chart recorder to the mini DIN socket Analogue out 0 1 V using the cable supplied Pin designations for the signals are as follows Pin no Signal Range Value full scale 1 UV 0 1V 0 001 5 au 2 signal ground OV 3 conductivity 0 1V 0 1 mS cm 999 9 mS cm 4 signal ground OV 5 pH 0 1V 0 14 6 signal ground OV Note The signal cable is delivered with protective covers on each wire Do not remove the protective covers from unused connections as a short circuit may disturb the measurements 2 Set the recorder to 0 1 V input full scale 2 7 Connecting to a communication link CAUTION The mains power to AKTA design must be switched OFF before connecting the module to the UniNet 1 link The monitor can be used as a stand alone module or in AKTA design As a stand alone module it is controlled from the front dial and display In AKTA design the monitor is controlled from a PC running UNICORN version 3 0 or higher via UniNet 1 cables or manually from the front dial and display Monitor UPC 900 User Manual 18 1125 55 Edition AG Installation A C
30. ars when the reading is stable and the following text is then shown Calibr pH Buffer 1 7 Adjust the pH value in the display using the dial so that it corresponds to the known pH value of the first buffer solution and press OK Calibr pH Buffer 2 8 Turn the dial clockwise to access the buffer 2 calibrating menu Rinse the electrode tip with distilled water and then immerse the electrode in the second buffer solution e g pH 4 0 or 9 0 and press OK A Monitor UPC 900 User Manual 18 1125 55 Edition AG 17 Operation Calibr pH Buffer 2 18 9 This text disappears when the reading is stable and the following text is hen shown Calibr pH Buffer 2 10 Adjust the pH value in the display using the dial so that it corresponds to he known pH value of the second buffer solution and press OK 11 When the two point calibration is performed successfully the following ext is shown for one second Calibration OK 12 From the Calibrate pH Buffer 2 menu turn the dial one step clockwise o access sub menu Calibrated Electrode Slope This menu shows the slope of the calibration curve where 100 corresponds to 59 16 mV per pH step at 25 C The asymmetry potential at pH 7 is shown as a mV value Press ESC to return to the Set Parameters menu Voltage mV 500 mV Asymmetry potential at pH 7 0 mv 500 mV 13 Before use rinse the electrode using distilled water A new electrode typically has
31. be air in the flow cell Use a flow restrictor after the flow cell and check that the flow restrictor gives a back pressure of 0 2 MPa Check for leaking tubing connections Check that the column is equilibrated If necessary clean the column Check the operation of the mixer and the pump Clean the flow cell according to the procedure in section 4 5 or 4 6 ne wem pa Clean the flow cell according to the procedure in section 4 5 or 4 6 2 The ambient temperature may have changed Use a temperature compensation factor see section B 2 4 Setting up conductivity to change temperature compensation in Reference information 1 Turn the flow cell so the end with the screws is facing the pH flow cell Recalibrate the conductivity cell 3 Calibration solution 1 00 M NaCl not correctly prepared Prepare a new calibration solution and recalibrate the conductivity cell Monitor UPC 900 User Manual 18 1125 55 Edition AG 29 Trouble shooting FF a Fault Action Ghost peaks appear 1 Acharged sample has been detected e g protein in the gradient profile 2 Air bubbles are passing through the flow cell Check for loose tubing connections If necessary use a flow restrictor after the conductivity flow cell Other problems Error in external pH or 1 Check the chart recorder in accordance with its manual Cond chart recorder 2 Test the recorder function and input voltage which should be 1 V full scale 3 Check the conductivity sc
32. ce temperature 39 B 2 6 Setup adjust conductivity 39 B 2 7 Setup adjust cell constant ec cecccssccseeeeeeeee 41 B 2 8 Setup show conductivity 41 B 2 9 Setup pH temperature compensation 42 B 2 10 Setup show pH 42 B 2 11 Setup UV averaging filter constant 42 B 2 12 Setup lamp run time 43 B 2 13 Setup show UV 43 B 2 14 Setup language 43 B 2 15 Setup unit nUMbEr 44 B 2 16 Setup display angle 44 B 3 Setting and using the alarm timer 45 B 4 Service displays B 5 Menu overvieW c sssssssssssssecccccennsssssescscegceensssssesceeegeennnses Technical SPECIFICATIONS essssssesscsscscssssssssesssssccssssssnueesescenssssnneesees 48 CL Operating AO nn 48 C2 Physical datant 49 Accessories and Consumables 0 eeccssssssssssssssssssssnnsseetsssesseeeeeeeees 51 Sh rtinstr cti ns neeeunaikannie a 57 About this manual This manual comprises two parts a practical part sections 1 5 and a reference part sections A D Sections 1 5 contain the necessary information for operating the instrument Monitor UPC 900 User Manual 18 1125 55 Edition AG VII Introduction 1 Introduction 1 1 General Monitor UPC 900 is a high precision on line monitor for the combined measurement of UV absorption pH and conductivity in liquid chromatography The UPC 900 features e Fixed wavelengths of 254 280 nm Hg lamp or 214 nm Zn lamp e Other wavelengths of 313 365 405
33. e under US pat 5 284 933 and US pat 5 310 663 including corresponding foreign patenst assigne Hoffman La Roche Inc All third party trademarks are the property of their respective owners 1998 2010 General Electric Company All rights reserved First published Apr 1998 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 18 1125 55 AG 10 2010
34. een pH and the output signal from the pH electrode is temperature dependent For accurate measurements during temperature changes the pH measurement can be temperature compensated In normal applications when the temperatures of the buffers and calibration buffers are identical temperature compensation is not necessary When using temperature compensation it is important that the temperature of the pH electrode is the same as that of the conductivity flow cell since that is where the temperature is measured 1 Select sub menu Setup pH and press OK 2 Select sub menu Setup pH Temp Comp and press OK The current status for showing pH is displayed If on is shown Te is displayed in main menus 1 and 2 If off is shown default Te is not displayed in the main menus 3 Change the setting if desired and press OK B 2 10 Setup show pH Normally the pH is displayed in the main operating menus see section 3 13 Reading pH temperature and conductivity values If not required the pH display can be set to off 1 Select sub menu Setup pH and press OK 2 Select sub menu Setup Show pH and press OK The current status for showing pH is displayed If on is shown current pH is displayed in main menus 1 and 2 If off is shown no pH is displayed in the main menus 3 Change the setting if desired and press OK B 2 11 Setup UV averaging filter constant 1 Select sub menu Setup UV and press OK 2 Select sub menu Set Averaging and press OK The
35. ence information 3 B 2 7 Setup adjust cell constant After replacing the flow cell the cell constant has to be set The cell constant is shown on the packaging 1 Select sub menu Setup Cond and press OK Select sub menu Setup Adjust Cell Const and press OK The current cell constant is shown A warning message is shown until confirmed by pressing OK The current cell constant value is displayed as default Enter the new cell constant as read from the packaging and press OK The range is 0 1 300 0 cm 1 When entering the cell constant from UNICORN select System Control System Calibrate and select Cond_Cell B 2 8 Setup show conductivity 1 Select sub menu Setup Cond and press OK Select sub menu Setup Show Cond and press OK The current status for showing conductivity is displayed If on is shown current conductivity is displayed in main menus 1 and 2 If off is shown no conductivity is displayed in the main menus Change the setting if desired and press OK Monitor UPC 900 User Manual 18 1125 55 Edition AG 43 Setup Temp Cond pH UV Setup pH Temp Comp off Setup pH Temp Comp off on off Setup Temp Cond pH UV Setup Show pH on Setup Show pH on on off Setup Temp Cond pH UV Set Averaging 1 35 13 Set Averaging 1 35 0 64 44 ED Reference information B 2 9 Setup pH temperature compensation The relationship betw
36. escribed in the User Manual e connected to other CE labeled GE Healthcare modules or other products as recommended This product meets the requirement of the Low Voltage Directive LVD 73 23 EEC through the following harmonized standards e EN 61010 1 e IEC 61010 1 e CAN CSA C22 2 No 61010 1 e UL61010 1 This device meets the requirements of the EMC Directive 89 336 EEC through the following harmonized standards e EN 61326 emission and immunity e EN 55011 GR 2 Class A emission e This device complies with part 15 of the FCC rules emission Operation is subject to the following two conditions 1 This device may not cause harmful interference 2 This device must accept any interference received including interference that may cause undesired operation Monitor UPC 900 User Manual 18 1125 55 Edition AG Reference information Cr D Accessories and spare parts Item Quant pack A C Code no Hg optics with 254 280 nm filters 1 A 18 1128 20 Zn optics with 214 nm filter 1 A 18 1128 21 Hg lamp amp housing complete 1 C 18 1128 22 Zn lamp amp housing complete 1 18 1128 23 UV flow cell 5 mm 1 18 1128 24 UV flow cell 2 mm 1 18 1128 25 Filter 214 nm 1 C 18 0622 01 Filter 254 nm 1 C 8 0620 01 Filter 280 nm 1 C 8 0621 01 Filter 313 nm 1 C 18 0623 01 Filter 365 nm 1 C 18 0624 01 Filter 405 nm 1 C 8 0625 01 Filter 436 nm 1 C 18 0626 01 Filter 546 nm 1 C 18 0627 01
37. evel value Press OK to acknowledge Press ESC twice to return to menu Set Parameters The module has an automatic over range function If the monitor signal reaches the full range value during a peak the signal will drop instantly to 0 V and give an accurate display of the peak starting from this position 3 9 Calibrating conductivity The cell constant for the particular flow cell is written on the flow cell packaging Refer to section B 2 in Reference information for how to enter the cell constant Adjustment of the cell constant is only necessary when the monitor is to be used to determine conductivity with high accuracy The procedure is described in Reference information section B 2 Calibration can also be performed from UNICORN Select System Control System Calibrate 3 10 Calibrating the pH electrode A good laboratory routine is to calibrate the module once a day when the electrode is replaced and if the ambient temperature changes The pH electrode is calibrated using standard buffer solutions in a two point calibration The two buffer solutions can have any pH value as long as the difference between them is at least 1 pH unit Calibration can also be performed from UNICORN In UNICORN select System Control System Calibrate Select the pH monitor The calibration procedure can be done with the pH electrode either fitted in or removed from the flow cell Monitor UPC 900 User Manual 18 1125 55 Edition AG Operation
38. he pH electrode connection Clean the pH electrode If problem remains change the pH electrode Factory calibration for pH is lost The monitor can still be used but may not meet specifications for pH measurement Call for service Factory calibration for conductivity is lost The monitor can still be used but may not meet specifications for conductivity measurement Call for service Only visible to service personnel Electrode slope is out of range Check buffers and recalibrate Clean the pH electrode and recalibrate If the message remains replace the pH electrode The difference between the pH of the buffers used during calibration must be at least 1 pH unit Check that the conductivity cell is connected Recalibrate The measured temperature value differs from the reference value by more than 5 C or actual temperature is lower than 8 C Recalibrate If the message remains replace the conductivity cell The difference between the zero level and full scale must be at least 1 pH unit Increase the span between zero and full scale settings See section 3 8 Monitor UPC 900 User Manual 18 1125 55 Edition AG Trouble shooting mi 98 Cal failed Cell 1 Conductivity cell constant is out of range Constantino 015300 2 Wrong solution used during calibration Use 1 00 M NaCl and recalibrate 3 Air in conductivity cell during calibration Flush the flow cell with calibration solution and recalibrate 4 Dirty
39. hile the monitor is running Before changing a UV lamp ensure that the lamp cable is disconnected from the monitor to prevent injury to eyes If the mercury lamp is broken make sure that all mercury is removed and disposed of according to national and local environmental regulations WARNING When using hazardous chemicals all suitable protective measures such as protective glasses must be taken WARNING HCI and NaOH are injurious to health Avoid spillage WARNING Hg lamps contain small amounts of mercury and must be handled with care and disposed of according to national or local environmental regulations WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe opera tion and maintenance of the system Monitor UPC 900 User Manual 18 1125 55 Edition AG Installation 2 Installation 2 1 Unpacking Unpack the module and check the items against the supplied packing list Inspect the items for obvious damage that may have occurred during transportation Note It is important that the filters flow cells and lamps are not handled during unpacking For protection of these items they should remain in their packing materials until required for use We recommend that all packing materials be retained if onward transport of the module is e
40. l on the filter wheel for the filter to be used A click will indicate that the filter is in position Note The wavelength and the flow cell type should also be entered in the Questions menu in UNICORN Filter change The Hg optics with 254 and 280 nm filters and the Zn optics with the 214 nm filter are delivered with filters installed If other filters are to be used install the new filters as described in 2 10 Installing optical filters optional Monitor UPC 900 User Manual 18 1125 55 Edition AG 5 Installation Protective cover 5 mm 2mm flow cell flow cell PET Holder Conductivity t cell e Clamp I PET Connection to the column 1 Fix the optical unit directly under the column if possible Note Always position the optical unit with the filter wheel cover facing upwards 2a Connect the column outlet tubing directly onto the top a of the optical unit for the 5 mm flow cell using a fingertight connector 2b Connect the column outlet tubing onto the bottom b of the optical unit for the 2 mm flow cell using a fingertight connector Note The inlet port of the 5 mm UV cell is above the optical unit The inlet port of the 2 mm UV cell is below the optical unit 3 Screw to fingertightness 4 Connect the optical unit outlet tubing onto the opposite hole in the flow cell Use fingertight connectors If no outlet tubing exists cut a piece of PEEK tubing i d 0 5 mm o d 1 16
41. lectronic equipment must not be disposed as unsorted municipal waste and must be mmm collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment WARNING This is a Class A product In a domestic environment this product may cause radio interference in which case the user may be required to take adequate measures Contents UV Contents 1 Introduction 1 1 GRNCTO EE ne nenne 1 E E E a et A seate 2 2 Installation 21 Uppa ennen aaa 3 2 2 General precautiDns 3 2 3 Installing the optical unit 4 2 4 Installing the conductivity flow cell 6 2 5 Installing the pH flow cell and electrode 6 2 6 Connecting electrical signal cables 8 2 7 CONNECTING to a communication link 8 2 8 Connecting to supply voltage 9 2 9 Preparing the module for use 9 2 10 Installing optical filters optional 10 3 Operation 3 1 Onl lteseseeaern ee 11 3 2 Menu selection and settings 11 3 3 Main menu OVEFVIEW re 13 3 4 Controlling the UV lampes 13 3 5 Reading the UV absorbance value 14 3 6 Autozeroing UV 3 7 Creating eventmarks 3 8 _ Setting Cond pH and UV analogue outputs eee 14 3 9 Calibrating conductivity seen 16 3 10 Calibrating the pH electrode 16 3 11 Filtering noise in the UV signal 19 312 Ehanging UVIOWCell seusseeee eat 19 3 13 Reading pH temperature and conductivity values 20
42. m the monitor to prevent injury to eyes If the mercury lamp is broken make sure that all mercury is removed and disposed of according to national and local environmental regulations Lamp housing end plate 1 Use a Philips screwdriver to detach the end plate by removing one and loosening the other of the two holding screws on the lamp housing to be removed 2 Slide the lamp housing off the filter housing 3 Detach the end plate as in step 1 above from the lamp housing to be fitted to the optical unit 4 Slide the lamp housing onto the filter housing The lamp and signal cables should be on the same side As you slide the lamp housing into position depress the two pressure pads on the filter housing in sequence to facilitate the installation 5 Refit the lamp housing end plate Dot on lamp housing 6 Slide the lamp housing firmly into place There will be a faint click when the housing is positioned correctly The Hg lamp housing can take up two positions one for 280 nm marked g by on the filter housing and N Symbols on the other marked by for all other wavelengths The Zn lamp housing filter housing has only one position es 7 Set the wavelength to be used by selecting lamp position indicated by below lid a dot on the lamp housing in combination with the appropriate filter i e the dot on the lamp housing should be adjacent to the symbol on the filter housing corresponding to the symbo
43. main operating menu 1 shows UV absorption conductivity in percentage of full range and pH If the pH value is not stable or is changing an asterisk is displayed i e pH This menu is reached from any other menu by pressing ESC If temperature compensation is switched on the display will show Cond Tc instead of Cond see section B 2 4 Setup conductivity temperature compensation in Reference information By turning the dial three steps clockwise main operating menu 2 is shown instead This display shows pH temperature in the conductivity flow cell and the actual conductivity in mS cm together with the percentage value If temperature compensation is switched on Te is shown in the display The display of pH temperature and conductivity can be disabled see Section B 2 of Reference information 3 14 Storage and shut down Storage of the UV flow cell CAUTION Do not allow solutions which contain dissolved salts proteins or other solid solutes to dry out in the flow cell Do not allow particles to enter the flow cell as damage to the flow cell may occur Overnight The flow cell can be left filled with buffer Weekend and long term storage Flush the flow cell with distilled water and then fill it with 20 ethanol The flow cell can also be stored dry by flushing as above with distilled water and then blowing a compressed inert gas such as nitrogen N through the cell Replace the red protective caps Never use compre
44. materials UV flow cells cond flow cell pH electrode flow cell dummy electrode pH stability range Typical lamp lifetime Hg 254 nm room temperature cold room Hg 280 nm room temperature Zn 214 nm room temperature Monitor UPC 900 User Manual 18 1125 55 Edition AG Reference information Stand alone or from a PC with UNICORN version 3 0 or higher via UniNet 1 connection IP 20 IP 21 flow cells IP 44 100 240 V AC 50 60 Hz 45 VA Languages available English German Spanish French Italian 1 5 m AMP 5 2 pole connector 1 5 m RJ 45 connector 1 5 m BNC connector 1 5 m D sub 9 pole connector Fingertight connector 1 16 0 1 V full scale 2 rows with 20 characters each 100 x 260 x 370 mm 8 5 kg Quartz ETFE titanium Titanium CTFE Glass FFKM perfluororubber titanium PTFE polytetrafluoroethylene 1 13 1 14 lt 1 day exposure 7000 hours 2000 hours 3500 hours 2000 hours 53 Reference information Chemical resistance Compliance standards Safety standards EMC standards 54 The wetted parts are resistant to organic solvents and salt buffers commonly used in chromatography of biomolecules except 100 ethylacetate 100 hexane and 100 tetrahydrofuran THF The declaration of conformity is valid for with the instrument only if it is e used in laboratory locations e used in the same state as it was delivered from GE Healthcare except or alterations d
45. n 90 Deconex 11 RBS 25 or equivalent and continue to squirt five more times 2 After five squirts leave the detergent solution in the flow cell for at least 20 minutes 3 Pump the remaining detergent solution through the flow cell 4 Rinse the syringe and then flush the flow cell with distilled water 10 ml 24 Monitor UPC 900 User Manual 18 1125 55 Edition AG Maintenance 4 5 Cleaning the conductivity flow cell in place ee WARNING NaOH is injurious to health Avoid spillage WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system Remove the pH electrode and install the dummy electrode in the pH flow cell Pump a cleaning or sanitising agent through the flow cell The standard recommendation is to pump 1 M NaOH for 30 minutes and then wash out with buffer 4 6 Cleaning the conductivity flow cell off line WARNING NaOH is injurious to health Avoid spillage WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system gt gt If the conductivity measurements are not comparable to previous results the
46. nce WARNING Always disconnect the power supply before attempting to replace any item on the module during maintenance CAUTION Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the module 4 1 Periodic maintenance Interval Action see procedures below Every 3 months Check the monitor Every 6 months Change the pH electrode or more often if required Clean the UV flow cell When required Clean the conductivity cell Clean the pH electrode 4 2 Cleaning and checking the module Wipe the monitor housing regularly with a damp cloth Let the monitor dry completely before use Lamp intensity Check Lamp Intensity 1 Select menu Check and press OK 0 00002 015 0 4 02 2 Select menu Check Lamp Intensity If R lt 300 mV for 254 nm R lt 150 mV for 280 nm R lt 150 mV for 214 nm replace the lamp according to Section 4 10 Changing the UV lamp or contact GE Healthcare for lamp replacement Lamp run time Check Lamp Run Time 1 Select menu Check and press OK Hg 2300h Zn 340h 2 Select menu Check Lamp Run Time e The lifetime of a Hg lamp at 254 nm in room temperature is typically 7000 hours in cold room typically 2000h e The lifetime of a Hg lamp at 280 nm in room temperature is typically 3500 hours e The lifetime of a Zn lamp is typically 2000 hours in room temperature Monitor UPC 900 User Manual 18 1125 55 Editi
47. ncerning the decommissioning of SS your equipment 26 Monitor UPC 900 User Manual 18 1125 55 Edition AG Trouble shooting 5 Trouble shooting UPC 900 Version 1 00 IN UV measurement No text on the front display Noisy UV signaldrift or instability Ghost peaks 5 1 General When contacting GE Healthcare for support state the program version of the module which is shown for 2 seconds during start up WARNING The module must not be opened by the user It contains high voltage circuits that can deliver a lethal electric shock 5 2 Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment 5 3 Faults and actions If the suggested actions do not correct the fault call GE Healthcare 1 Check that the mains cable is connected and the power switch is in ON position I 1 Select menu Check Autozero to check the autozero absorbance value If AZ is between 1 5 and 2 there may be air bubbles in the flow cell or the wrong buffer system in use 2 Wrong filter for the lamp used Check that the lamp is in proper position and that the correct filter is used 3 The buffer may be impure Check if the signal is still noisy with water There may be air
48. nd press OK The current temperature is shown 5 A warning message is shown until confirmed by pressing OK 6 The current adjustment value is displayed as default Enter the temperature shown on the thermometer and press OK Monitor UPC 900 User Manual 18 1125 55 Edition AG Setup Temp_ Cond pH UV Setup Show Temp on Setup Show Temp on on off Setup Temp Cond pH UV Setup Cond Temp Comp 0 0 Setup Cond Temp Comp 0 0 0 0 Setup Temp Cond pH UV Setup Cond Ref Temp 25 0 C Setup Cond Ref Temp 25 0 25 0 Reference information 3 B 2 3 Setup show temperature The display of the temperature in the conductivity flow cell shown in the main operating menu 2 can be enabled or disabled 1 Select sub menu Setup Temp and press OK 2 Select sub menu Setup Show Temp and press OK The current status for showing temperature is displayed If on is shown current temperature is displayed in main menu 1 and 2 If off is shown no temperature is displayed in the main menus 3 Change the setting if desired and press OK B 2 4 Setup conductivity temperature compensation 1 Select sub menu Setup Cond and press OK 2 Select sub menu Setup Cond Temp Comp and press OK The current temperature compensation factor is shown 0 0 means that the compensation is off default setting The range is 0 0 9 9 3 The current compensation factor is displayed as
49. nts we recommend that the pH electrode is removed and the dummy electrode inserted in its place as organic solvents will cause pH electrode degeneration Monitor UPC 900 User Manual 18 1125 55 Edition AG 9 Installation 10 2 10 Installing optical filters optional The Hg optics with 254 and 280 nm filters and the Zn optics with the 214 nm filter are delivered with filters installed If other filters are to be used install them as follows 1 If the Zn lamp is attached remove the lamp housing as described in section Changing the UV lamp Remove the four screws in the filter housing Separate the filter housing from the detector housing Carefully remove the filter wheel from the filter housing If necessary remove the filter s from the filter wheel by pressing it them out e g with a small screwdriver Note Filters are sensitive optical components Never touch the optical 10 surfaces or expose them to temperatures above 60 C Clean them with dry lens cleaning tissue and store them when not in use in the box in which they were supplied Heavy contamination may be removed by using a lens tissue dipped in ethanol Insert the filter s of choice into the filter wheel maximum 3 filters with the correct orientation the mirror side facing upwards and position over one of the three triangular apertures The filters snap in by pressing them quite firmly Do not touch the filter surface Remove the circula
50. on AG 23 Maintenance When necessary replace the lamp according to Section 4 10 Changing the UV lamp or contact GE Healthcare for lamp replacement Autozero The module internal absorbance value for autozero can be checked to test the consistency of buffers 1 Select menu Check and press OK 2 Select menu Check Autozero The autozero absorbance value for the wavelength used is shown 4 3 Cleaning the UV flow cell in place WARNING NaOH is injurious to health Avoid spillage WARNING When using hazardous chemicals take all suitable protective measures such as wearing protective glasses and gloves resistant to the chemicals used Follow local regulations and instructions for safe operation and maintenance of the system gt gt Pump a cleaning or sanitising agent through the flow cell The standard recommendation is to pump 1 M NaOH for 30 minutes and then wash out with buffer 4 4 Cleaning the UV flow cell off line A clean flow cell is essential for ensuring the correct operation of the UV monitor CAUTION Do not allow solutions that contain dissolved salts proteins or other solid solutes to dry out in the flow cell Do not allow particles to enter the flow cell as damage to the flow cell may occur 1 Connect a syringe to the inlet of the flow cell and squirt distilled water through the cell in small amounts Then fill the syringe with a 10 surface active detergent solution like Deco
51. on solution by pumping at least 15 ml through the cell with a syringe 3 Stop the flow and wait 15 minutes until the temperature is constant in the range 20 30 C 4 Read the conductivity value displayed and compare it with the theoretical value from the graph below at the temperature of the calibration solution If the displayed value and the theoretical value correspond no further action is required If the values differ proceed with actions 5 8 Setup 5 Select sub menu Conductivity of 1 00 M NaCl at 20 30 C ERP cen RHEIN Setup Cond and press OK 97 Setup Adjust Cond 6 Select sub menu 1 000mS cm y Setup Adjust Cond 95 and press OK The current conductivity value is shown Warning This will 7 A warning message change cell calib is shown until confirmed by 90 pressing OK 8 The current value Setup Adjust Cond es 83 53mS cm 86 6 is displayed as default Enter the theoretical conductivity value according to the graph and press OK The new cell constant is automatically calculated The range is 1 000 999 9 mS cm 85 80 77 42 Monitor UPC 900 User Manual 18 1125 55 Edition AG Setup Temp Cond pH UV Setup Adj Cell Const 83 56cm 4 Warning This will change cell calib Setup Adj Cell Const 83 56 1 866 Setup Temp Cond pH UV Setup Show Cond on Setup Show Cond on on off Refer
52. onnect two UniNet 1 cables to the UniNet 1 connectors The module can be connected in series anywhere between the PC and a termination plug The UniNet 1 link connects in series the PC with Pump P 920 Monitor UPC 900 and the Frac 920 The termination plug is connected to the last module in the chain 2 8 Connecting to supply voltage A WARNING The module must be connected to a grounded mains socket 1 Make sure the on off switch is in the OFF position 0 2 Connect the supplied mains cable between the module and a grounded mains socket Any voltage from 100 240 V AC 50 60 Hz can be used Note The module contains no user replaceable fuse 2 9 Preparing the module for use Before performing the following procedures we recommend you read sections 3 1 3 3 e Calibrate the pH electrode see section 3 6 e Set the conductivity cell constant see section B 2 3 Only required when the flow cell has been replaced e Calibrate the temperature sensor see section B 2 6 Only required if the monitor is to be used for high accuracy measurements or if the flow cell has been replaced Note The conductivity cell constant is shown on the packaging Retain the packaging in case the conductivity cell constant needs to be re entered Note The measured temperature is the temperature in the conductivity flow cell which can differ from the ambient temperature in AKTA design systems Note When running chromatography using organic solve
53. orted Because of this the averaging time should be as short as possible On delivery the averaging time is set to 1 3 s Setup 1 From main menu 1 turn the dial six steps clockwise and press OK twice Setup 2 Select Setup UV and press OK Set Averaging 3 The menu Set Averaging is displayed showing the current set averaging eal time Press OK Set Averaging 4 Set the desired value and press OK Values allowed are 0 02 0 04 0 08 13s ge 0 16 0 32 0 64 1 3 2 6 5 and 10s Averaging time s Corresponding Min peak width time constant s at half height s approximately 10 0 5 00 50 0 5 10 2 00 32 0 2 60 1 00 16 0 1 30 0 50 8 0 0 64 0 20 3 2 0 32 0 10 1 6 0 16 0 05 0 8 0 08 0 03 05 0 04 0 01 0 2 0 02 0 01 0 1 In UNICORN the averaging time is set with the instruction AveragingTime in System Control Manual Alarm amp Mon 3 12 Changing UV flow cell The flow cell can be changed when required for example from 2 mm to 5 mm when the sensitivity of the measurement must be increased due to a small amount of sample being applied or from 5 mm to 2 mm when a lower sensitivity is desired due to output signal limitation See section 2 3 Installing the optical unit sub section Flow cell installation Monitor UPC 900 User Manual 18 1125 55 Edition AG 19 Operation AU Cond Tc pH 0 00002 015 0 4 02 pH 12 50 22 4 735 8 mS cm Tc78 8 20 3 13 Reading pH temperature and conductivity values The
54. r plastic band showing the wavelength s Remove labels from the band if necessary Place the correct labels on the band with the label designation facing outwards Ensure that the label position corresponds to the filter position i e the label should be placed opposite the filter Reassemble the circular plastic band with the filter wheel peg fitting into the band notch Check that all filters are clean Insert the filter wheel back into the filter housing Note The filter wheel can be placed only in correct position 11 Reassemble the filter housing with the detector housing by fastening the four screws Monitor UPC 900 User Manual 18 1125 55 Edition AG Operation 3 Operation 3 1 On off Selftest Switch on the module at the mains switch on the rear panel At switch on Please wait the module performs a selftest Several beeps are heard during this process If an error is detected an error message is shown UPC 900 version hos Name and software version number is shown for 2 seconds Salibrating All parameters are set to factory default values Calibration ok AH Gondi IPM The selftest takes approx 1 minute When start up is completed with no errors the display shows the main menu 1 The monitor can be used immediately but the full specifications are not obtained until after 1 hour of lamp warm up 3 2 Menu selection and settings Menu selection A specific men
55. response differs greatly the electrode may require cleaning or replacement Monitor UPC 900 User Manual 18 1125 55 Edition AG Fault Trouble shooting Action pH values vary with varied back pressure 10 There may be interference from static fields Connect the pH flow cell and the rear panel of the monitor using a standard laboratory 4 mm banana plug cable 11 Check that the pH electrode has been calibrated at the correct temperature 12 In organic solvents such as ethanol methanol and acetonitrile stable pH measurements are not possible since dehydration of the membrane will occur We recommend that the pH electrode is not used in applications using organic solvents Mount the dummy electrode instead 13 Clogged liquid junction Refer to section 4 8 Cleaning the pH electrode 1 Replace the pH electrode Conductivity measurement Incorrect or unstable reading Baseline drift or noisy signal Conductivity measurement with the same buffer appears over time Absolute conductivity value wrong 1 Check that the conductivity flow cell cable is connected properly to the rear ofthe module 2 Check that the pump operates correctly 3 If temperature compensation is being used check that the temperature sensor is calibrated and that the correct temperature compensation factor is in use 4 Check that the column is equilibrated If necessary clean the column 5 Check the operation of the mixer 1 There may
56. rm time and the count down timer The default or current value is shown in parentheses 1 Select main menu Alarm Timer The display shows current time 2 Press OK Turn the dial one step clockwise to select sub menu Set Alarm if you want to set an alarm at a fixed time Press OK to enter the time in the form HH MM SS and press OK again after entering each time unit 3 If you want to set a count down time turn the dial one step further to select sub menu Set Timer Press OK to enter the count down value in the form HH MM SS and press OK again after entering each time unit 4 Press ESC to return to the Alarm Timer menu which now shows the set alarm time or count down time as BzzHH MM SS 5 When the alarm time is due or the count down timer reaches 00 00 00 an alert display is shown and the module beeps for 30 s or until OK is pressed The display shows the time elapsed since the alarm and the current time A second alert display is shown until OK is pressed The alarm timer is based on the internal module clock which can be set in the Set Clock menu located after the Set Timer menu The clock will be reset when power is turned off A set alarm timer function can be reset by pressing OK in the menu Alarm Timer off B 4 Service displays The module has service displays for use by authorised service personnel If the service display Insert Access Code is accidentally selected press ESC to return to the normal operation display
57. s and the resulting current is measured and used to calculate the conductivity of the eluent The monitor controls the AC frequency and increases it with increasing conductivity between 50 Hz and 50 kHz giving maximum linearity and true conductivity values The conductivity is automatically calculated by multiplying the measured conductance by the flow cell s cell constant The cell constant is pre calibrated on delivery but can be measured with a separate calibration procedure This procedure is described in Reference information section B 2 One of the electrodes has a small temperature sensor for measuring the temperature of the eluent in the flow cell Temperature variations influence the conductivity and in some applications when highly precise conductivity values are required it is possible to program a temperature compensation factor that recalculates the conductivity to a set reference temperature Temperature sensor Eluent gt Monitor UPC 900 User Manual 18 1125 55 Edition AG 37 Reference information 38 Monitor UPC 900 User Manual 18 1125 55 Edition AG Reference information 3 B Menus B 1 Check menus B 1 1 Checking autozero level The module internal absorbance value for autozero can be checked to test the consistency of buffers Check Autozero 1 Select menu Check and press OK AZ 0 00006 AU 2 Select sub menu Check Autozero The autozero absorbance value for the used wavelength is shown
58. so for this wavelength the light is converted at a fluorescent surface before it passes the filter On the lamp housing there is a special exit for 280 nm light which means that the lamp position needs to be changed when working with this wavelength For 214 nm wavelength a Zn lamp is used This lamp is larger than the Hg lamp and is therefore mounted in a larger lamp housing The lamp connectors are keyed to inform the monitor software of which lamp type is connected Monitor UPC 900 User Manual 18 1125 55 Edition AG KES Reference information A A 3 pH electrode 9 The pH electrode is of the sealed combination Sealing double junction type It contains a sealed Ag AgCI SS prige conania reference which cannot be refilled an internal INS with Agel electrolyte bridge of 4 M KCI saturated with Ag LL ru AgCI an outer electrolyte bridge of 1 M KNO I electrode Internal annular He nal annul an annular ceramic reference junction and a chi San low profile pH membrane The pH electrode is me delivered with a transparent cover om 4 Glass Secale j containing diluted The flow cell should not be used with any other ee KCI pH electrode External annular coaxial ceramic reference junction A 4 Conductivity cell The flow cell has two cylindrical titanium electrodes positioned in the flow path of the cell An alternating voltage is applied between the electrode
59. ssed air as this may contain droplets of oil Storage of the conductivity flow cell Overnight The conductivity cell can be left filled with a buffer Weekend or long term storage Flush the conductivity cell with water and fill with 20 ethanol Monitor UPC 900 User Manual 18 1125 55 Edition AG Operation Storage of the pH electrode CAUTION Never leave the pH electrode in the flow cell for any period of time when the system is not used since this may cause the glass membrane of the electrode to dry out Dismount the pH electrode from the flow cell and fit the end cover filled with a 1 1 mixture of pH 4 buffer and 2 M KNO Do NOT store in water only The pH electrode should always be stored in a 1 1 mixture of pH 4 buffer and 2 M KNO when not in use When the pH electrode is removed from the flow cell the dummy electrode supplied can be inserted in the flow path Electrode regeneration If the electrode has dried out immerse the lower end of the electrode in buffer with a 1 1 mixture of pH 4 buffer and 2 M KNO overnight 3 15 Restart after power failure If the power supply to the module is interrupted it automatically restarts when power is restored and displays the main operating menu All set values are retained in the module and the lamp is switched on Monitor UPC 900 User Manual 18 1125 55 Edition AG 21 Operation 22 Monitor UPC 900 User Manual 18 1125 55 Edition AG Maintenance 4 Maintena
60. u is selected by turning the front selection dial clockwise or counter clockwise When the required menu is visible the menu or selection is accepted by pressing the OK button Menu selection OK button ESC button Go down one menu level Return one menu level or accept setting If a menu has sub levels the sub menu is displayed by pressing OK Pressing ESC moves back one menu level Monitor UPC 900 User Manual 18 1125 55 Edition AG 11 Operation 12 Main menus Sub menus 3 click cw ESC or 3 click with the dial _JECw with the dial OK gt submenu2 1 ok gt Sub menu 2 1 1 lt ESC lt ESC Sub menu 2 2 OK gt Sub menu 2 3 1 Sub menu 2 3 ESC Sub menu 2 3 2 Return to main menu Pressing ESC repeatedly always returns to the main menu 1 which is the main operating menu Main menu 1 353 ESC ESC ESC ESC Main menu 2 Select value A cursor below a text or numerical value shows what is affected by the dial To increase a numerical value turn the dial clockwise To decrease the value turn the dial counter clockwise To simplify entering large numerical values the cursor moves to next digit if the dial is turned quickly in one direction The cursor moves back one place to the right every two seconds if the dial is not turned The text or numerical value displayed is accepted by pressing OK To cancel press ESC Monitor UPC 900 User Manual 18 1125 55 Edition AG
61. used to set measurement parameters zero and full range values for Cond pH and UV on the analogue output channels The analogue output channels can be connected to a chart recorder for example From the main menu 1 turn the dial four steps clockwise to enter the menu Set Parameters Click OK select the quantity to set by moving the cursor with the dial then click OK again Note The user interface of the monitor must be unlocked if connected to a UNICORN control system Setting the Cond analogue output 1 Current analogue settings are displayed zero and full scale values Monitor UPC 900 User Manual 18 1125 55 Edition AG Set Cond Zero Level Set Cond Zero Level Set Cond Full Scale Set Cond Full Scale Set ph Analog Out Set ph Full Scale Set ph Full Scale Set ph Zero Level Set ph Zero Level Set UV Analog Out Set UV Range Set UV Range Operation 2 Press OK to access the settings menu Press OK 3 Set the desired zero value The range is 0 000 999 9 mS cm Press OK to acknowledge 4 Turn the dial clockwise to access the settings menu Press OK 5 Set the desired full scale value The range is 0 000 99 9 mS cm Press OK to acknowledge Press ESC two twice to return to menu Set Parameters Setting the pH analogue output After having selected Set Parameters pH and pressed OK turn the dial one step clock
62. wide range of applications Connector switch Function UniNet 1 Computer network Analogue out 0 1 V Chart recorder outputs 3 channels 0 1 0 V Optical Unit UV signal from optical unit pH Probe Connection to pH electrode standard BNC socket pH Ground Reference ground for pH measurement Conductivity Flow Cell Connection to conductivity flow cell 9 pole D sub connector Lamp UV lamp connection Mains Supply voltage grounded 0 1 Mains supply on off switch The module contains no internal user replaceable items Monitor UPC 900 User Manual 18 1125 55 Edition AG 35 A Reference information 36 A 2 UV optical unit The UV optical unit houses the lamp Zn or Hg the wavelength filter and the UV flow cell The light beam is directed through a double conical or straight flow through cuvette 6 ml or 2 ml illuminated volume to a photodetector The photodetector current is fed to the signal processing circuitry in the module Optical Unit Inlet Filter Lens Beam splitter Flow cell Photodetector Lam P Outlet Photodetector eal UPC 900 Analogue outputs The reference signal comes from the same point in the lamp as the signal measuring the sample thus assuring a stable baseline by eliminating the effects of variations in lamp intensity The Hg lamp emits light only at certain wavelengths It does not emit light at 280 nm
63. wise to skip the Calibrate pH menu Then press OK Note The pH values for zero level and full scale must differ by at least 1 pH unit Note The zero level and full scale values can be calibrated in any order 1 Current analogue settings are displayed zero and full scale values 2 Press OK to access the full scale settings menu Press OK 3 Set the desired full scale value The range is pH 0 50 14 30 Press OK to acknowledge 4 Turn the dial clockwise one step to access the zero level settings menu Press OK 5 Set the desired zero level value The range is pH 0 50 14 30 Press OK to acknowledge Press ESC twice to return to menu Set Parameters Setting the UV analogue output 1 Current analogue settings are displayed zero and full range values Allowed full range values are 0 0001 0 0002 0 0005 0 001 0 002 0 005 0 01 0 02 0 05 0 1 0 2 0 5 1 0 2 0 and 5 0 Zero level is set as a percentage of full scale 2 Press OK to access the settings menu The current setting is displayed Press OK 3 Set the desired full range value Press OK to acknowledge Monitor UPC 900 User Manual 18 1125 55 Edition AG 15 Operation Set UV Zero Level Set UV Zero Level Measured absorbance level A U Range Over range AU Signal to recorder V 1V 16 4 Turn the dial clockwise to access the settings menu The current setting is displayed Press OK 5 Set the desired zero l
64. xpected Note pH measurement is optional in AKTA design CAUTION Read the following information carefully to ensure that the module is installed correctly 2 2 General precautions The module should be located in a place of low temperature variations away from heat sources draughts and direct sunlight The module may be operated at normal ambient temperatures in the range 4 to 40 C The module should be installed on a stable laboratory bench or in AKTA design system rack To ensure correct ventilation a free space of 0 1 m is required behind and in front of the module Do not place soft material under the module to ensure that the ventilation inlet below the front is not blocked UPC 900 TIJ Monitor UPC 900 User Manual 18 1125 55 Edition AG 3 Installation O ring Conductivity unit cell Protective plug for flow cell 2mm cm NCO 5mm Detector Screw Holder Optical unit 2 3 Installing the optical unit Installing the holder 1 Hook the holder into the slot on the right hand side of the module Secure it by pushing up the slide clamp Flow cell installation There is one analytical 5 mm and one preparative 2 mm flow cell available stand alone units only include the preparative flow cell Both cells are installed in the same way as described below 1 Remove the red protective plugs from the detector housing and

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