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AND1000 Fluorimeter for Water Testing

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1. Normal operation of this instrument may involve the use of hazardous chemicals or biologically harmful samples The user must observe all cautionary information printed on the original solution containers and safety data sheet prior to their use All waste solutions must be disposed in accordance with local and national law The type of protective equipment must be selected according to the concentration and amount of the dangerous substance at the specific workplace 3 General Information 3 1 Overview of Product The AND1000 Fluorimeter enables field testing to be done in two short steps eliminating or significantly reducing test expenditures complexity and wait times experienced in traditional heavy metals water testing The Fluorimeter uses an extremely sensitive measurement technique to determine the levels of heavy metal contaminants ANDalyze s proprietary Catalytic DNA sensors use a DNAzyme reaction that fluoresces in the presence of a target contaminant substance such as lead The fluorescence of the reaction is measured to determine the concentration of the target heavy metal and is reported in parts per billion ppb Using the product to test for metals is a simple quantitative test that allows for detection at or below US EPA standards in drinking water The test is performed by taking a 1 milliliter water sample injecting it through the sensor and into the AND1000 fluorimeter This sample is then automatic
2. CE Marked Complies with the following European Union Directives e Low Voltage Electrical Equipment Directive 2006 95 EC e Electromagnetic Compatibility EMC Directive 2004 108 EC Power Specifications Power Battery Operated Power Recharging via USB cable Power usage The battery lasts over 225 tests on a full charge at 45 seconds test Mechanical Specifications Dimensions Width 3 6 inches 9 14 cm Depth 8 0 inches 20 3 cm Height 2 25 inches 5 72 cm Weight 1 25 lb 565 g IP rating IP54 water resistant Data specifications Interface USB 2 0 Data Downloaded in csv format Note All specifications are subject to change without notice ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 15 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 10 Limited Warranty for Fluorimeter Device ANDalyze provides the following limited warranty for the ANDalyze AND1000 Fluorimeter device the Fluorimeter Device ANDalyze warrants that the Fluorimeter Device including the software used on the Fluorimeter Device when used in accordance with the user documentation will operate in all material respects in conformity with the specifications stated in the user documentation for a period of ninety 90 days from the date of your receipt the Warranty Period If it does not your sole remedy and ANDalyze s total liability for such ma
3. Sensor Details choose Select 5 4 Deleting a Site Sensor Lead Site N DEMO H 1 When in the Site Sensor Details Date Enler d 9717712 el manent ly delete screen using the left right keys scroll Canoe IT 12 site DEMO to Delete and select Water Temp 72 F or ALL Sensors 3 Sensor Lot 691712 z 2 On the Confirm Delete screen select Press 4b to change the Action Delete Berere gt Cancer Dere Cancer ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 12 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 5 5 Re Calibrating a Site Sensor Lead Lead x 7 Site N DEMO 1 When in the Site Sensor Details Date alore d 9717712 DEMO screen using the lef right keys scroll arska e Er E lease insert sample to Calibrate and select Water Temp 72 F ater spiked with i i Sensor Lot 691712 2 Follow instruction to calibrate Press 4b to change the Action Gibek gt Caney i Sar Caner 6 Maintenance CAUTION Always disconnect power from the instrument before attempting any cleaning operations Important Under no circumstances should the instrument display or the accessories be cleaned with solvents such as white spirit acetone etc Fluorimeter Clean the enclosure sample cell compartments and all accessories with a soft damp cloth A mild soap solution can also be used Do not get excess water in the sa
4. time of a site calibration This helps to identify when a new site calibration is required significant change in water temperature Temperature Units Switch between Celsius and Fahrenheit for temperature entry and displays Display Contrast Adjusts the brightness of the display Higher value indicates a brighter display Window 100 Samples 100 ale 433 41 Offset Delay R Press 4b to change the Action Save gt A Warning Please read the user manual before proceeding Wext Cancer ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 9 of 17 AND1000 Fluorimeter for Water Testing Scale Slope of the line that correlates the rate of fluorescence increase vs concentration This value is determined by the 3 point calibration Some sensors have a default scale that is automatically calculated from other sensor s scales In this case Auto will be indicated in the default scale field A new 3 point calibration will supersede the default settings Offset Intercept of the line that correlates the rate of fluorescence increase vs concentration Also automatically determined by the 3 point calibration 3 Point Lab Calibration Iced OCT 100 Using the left right keys in the Advanced menu Select the Calibrate option Window Samples cale Offset Delay R Press 4 gt to change the Action R C
5. 14 9 Technical Specification for AND1000 FIUOriMetel ccc cccsecescesseecessessesecssesseecaesescsssaeceeseeseseaseeeees 15 10 Limited Warranty cscs Gen ois sates Poesia oa den che te ca gs aU Saas ae ce RO 16 ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 2 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 2 2 1 2 2 Safety Information Please read this entire manual before unpacking setting up or operating this equipment Pay attention to all danger warning and caution statements Failure to do so could result in serious injury to the operator or damage to the equipment Make sure that the protection provided by this equipment is not impaired do not use or install this equipment in any manner other than that specified in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the manufacturer may be impaired Use of Hazard Information DANGER Indicates a potentially or imminently hazardous situation which if not avoided could result in death or serious injury CAUTION Indicates a potentially hazardous situation that may result in minor or moderate injury Important Indicates a situation which if not avoided may cause damage to the instrument Information that requires special emphasis Note Information that supplements points in the main text Precautio
6. 453 6740 By Fax 1 857 386 1277 Business Service Hours 9 00am to 5 00pm Eastern Standard Time USA Company Address ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Website www andalyze com 8 Consumables and Replacement Items Fluorimeter Kit o Capable of measuring multiple metals o Kit Includes Fluorimeter USB to MINI B Cable 100uL Fixed Volume Pipette and Tips pH Test Strips Sensor Kits o Equipment for 25 Tests and or Calibrations Kit Includes 25 Sensor Bags with Sensor amp Cuvette 25 Sample Tubes with buffer 25 1 mL Syringes October 1 2012 25 Disposable Transfer Pipettes Not included in High Range Copper 8 mL Analyte Standard Solution Instruction Manuals Material Safety Data Sheets MSDS ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 14 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 9 Technical Specification for AND1000 Fluorimeter The AND1000 Fluorimeter is a platform centric hand held device for heavy metals testing for drinking water and industrial water supplies It uses an extremely sensitive measurement technique to determine the levels of heavy metal contamination Instrument Specifications Light Source Light Emitting Diode Detector Photomultiplier Tube Filters Excitation 485nm Emission 535nm Certifications AND 1000 Fluorimeter
7. AN Dalyze AND1000 Fluorimeter for Water Testing User Manual October 2012 Edition 2 ANDalyze Inc 2011 All rights reserved Printed in US AND1000 Fluorimeter for Water Testing October 1 2012 Table of Contents 2 Safety Informatio Nismas tnn a a a a eaa eiii a 3 2 1 Use of Hazard Information 2 2 Precautionary labels 2 3 Chemical and Biological Safety 2 General iniormatio Mee e EEE O AE E E tae O 4 3 1 Overview of Product 3 2 General Features 3 3 Analyte Sensors 3 4 Operating Environment amp Storage 3 5 Water Testing Guidelines A Set Up and Operating Procedures cccsssssssssscssssecssssscsscsscescessessesssseessescasescasseesseseeseeseaaesessassaesansess 6 4 1 Charging the Battery 4 2 Home Testing Screen 4 3 Sensor Screen 4 4 Site Screen 4 5 Start Option 4 6 Menu Screen 4 7 Firmware Update 4 8 InstraComm Lite 52SiteSet Up and Manageme nt iener ia vent eascncecivecevetsvteesvesssesestdacciedbacdvars a ieii 11 5 1 New Site Set Up 5 2 On Site Calibration 5 3 Selecting a Site 5 4 Deleting a Site 5 5 Re Calibrating a Site 6 MaintenaNt Ene neniann anea wie cased acetal da aveco advan aa a dua lebhasvidedtuseudepeckdenacecteasbepursuarsivdense 13 7 Customer Service Contact INfFOrMAtiON cccesssesssssecssescescesceeceesaecaesesseseeeaesaesaeasseceecaecaecaeeeseesaesaeaaeaes 14 8 Consumables and Replacement ItOMS cccccceccsssescesssssessssscesescseseecaeseecsesecesesaaessesaeseescaesescassaeeaeseeses
8. Attach the USB to MINI B Cable to the outlet at the bottom of the device and to a USB powered source such as a computer or a USB charger See Photo to Right ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 6 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 4 2 Home Testing Screen Options can be accessed by Using the up down buttons and pressing SELECT button when the arrow is to the left of the desired option OR by pressing the button located just below the screen under the desired options at bottom Selectable Options 1 Sensor Selects which Sensor will be tested 2 Site Uses site specific calibrations for accurate tests 3 Start To begin a test of the indicated metal Time 16 84 14 55 Press SELECT to Edit 4 Menu To change or view internal settings Starr Meno 4 3 Sensor Screen Selecting the Sensor option allows the user to change the sensor which the meter will detect Once selected the Color of the area surrounding the name of the sensor and the photo of the sensor at the right on the Home Screen will match the color of the sensor itself used for testing Peer PERET 4 4 Site Screen Selecting the Site option allows the user to change or create new sites Sites are important because each water sample contains differences which affect testing results It is recommended that each location is ca
9. Once logged in you will see the option to update your device Follow the instructions ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 10 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 4 8 InstraComm Lite This software allows users to launch and record live sample results download all sample results from the instrument and perform related configuration tasks The InstraComm application was developed by Autonomous Innovations Inc and a complimentary Lite version has been made available with the purchase of ANDalyze s AND1000 To download your complimentary version of InstraComm Lite please visit andalyze com and log in to register your device For additional information and operating instructions see InstraComm Lite Solutions Notes Available separately 5 Site Set Up and Management This section provides information concerning the set up and management of Sites As different sample locations contain different water characteristics sample matrix it is recommended to create a new site for each sample area This allows the user to track and compare the results at each location while also normalizes the results through an on site calibration 5 1 New Site Set Up Sites are important because each water sample contains differences which affect testing results It is recommended that each location is site calibrated So by creating calibrating and
10. ally analyzed and reports results in less than two minutes ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 4 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 3 2 General Features Fluorescence Based Sensing Reaction produces quantitative fluorescence based results Sensor Kit Each disposable color coded sensor is designed for a specific heavy metal test target Kits provide sampling tubes syringes and cuvettes Rugged amp Convenient Construction Tough light weight impact resistant shell protects electronics and sensors Key Pad Quick simple menu navigation and one button push for sample analysis Data Capture amp Reporting Time and date stamped with sample number Stores over 240 000 test results and over 75 site locations Data downloadable through a USB cable Water Resistant Enclosure rating IP54 Portable Battery operated and rechargeable through USB cable or traditional adapter Note See full technical specifications in section 9 3 3 Analyte Sensors To help differentiate the materials specific to each analyte the packaging has been individually labeled and the sensor housings and buffer caps have been color coded For Example Green Lead Pb Orange Uranium U Blue Copper Cu Gray Mercury Hg Etc Note In some cases the same sensor is used for multiple ranges but different buffers and o
11. ally performed Do not rely ona calibration to be accurate over long time periods as environmental samples can vary greatly The On Site calibration process adjusts for changes in sample matrix such as ionic strength and pH by calculating the recovery of a known spike in that matrix When a user performs an on site calibration for Lead for example the fluorimeter software calculates the ppb of lead in the sample a and the ppb of lead in sample spiked with 25 ppb lead b using the calibration curve from the 3 point calibration initially completed at factory or in laboratory It then calculates an accuracy factor based on recovery Accuracy factor 25 b a This factor is stored for a particular site that the user enters The sample concentration displayed after an on site calibration a accuracy factor Important On Site calibration must be performed if testing is being done Ata new location which has not been previously saved To adjust for significant changes in sampling temperature When beginning to use a new lot of sensors See the specific Testing and Calibration Manual for detailed instructions 5 3 Selecting a Site Sensor lead Site Name DENO 1 Onthe Home screen select the Site Date Entered 9 17 12 option Calibrated 9 17 12 f s Sre Factor 3 22137 2 Using the up down keys highlight the Water Temp 72 F Sensor Lot 691712 desired site and press Select Press 4b to change the Action 3 On the Site
12. ancer Note This is for laboratory calibration ONLY and requires 3 standardized solutions from manufacturer or prepared by a laboratory technician 25 56 75 ppb JHE Cancer When all 3 solutions have been tested the calibration results screen will be displayed Click Apply to save the results The settings will then be viewable in the Advanced screen 4 7 Firmware Update October 1 2012 Delay Points from the start of the test which are not used for calculations This is used when there is a delayed reaction in certain sensors R2 R Squared is a statistical term indicating how good one term is at predicting another In our case this is derived from the best fit line created by the 3 point lab calibration Values closer to 1 are better Factory Reset The Reset option will restore the setting of that specific sensor to factory settings Using the left right keys in the Advanced menu Select the Reset option Leda 166 Window Samples 100 Scale 433 41 Offset Delay R Press 4 gt to change the Action b Select Reset on the Warning screen to complete the process A Warning You are about to perform a factory reset Current settings will be lost Reser Cancel ANDalyze is continually adding new features and options to the AND1000 through new firmware updates To update your AND1000 firmware please visit andalyze com and log in to register your device
13. e Inc 2109 S Oak Street Suite 102 Champaign IL 61820 USA Email info andalyze com Telephone 1 888 388 0818 or 1 217 328 0045 ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 17 of 17
14. librated So by selecting a site this calibration can be used repeatedly EZZ Please see section 5 below for further instructions on setting up and calibrating sites 4 5 Start Option Depending on your Options settings Step 1 of 4 pressing the Start button will either Fill the sample tube with k a start the testing instructions OR if sample water to the 5mL Progress turned OFF will begin the testing mark and mix ATTA immediately for advanced users WE E ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 7 of 17 AND1000 Fluorimeter for Water Testing 4 6 Menu Screen October 1 2012 The Menu Screen can be accessed by pressing the button located below the screen under Menu Selectable Options 1 Results 2 Settings 3 Options 4 Advanced Options are selectable using the arrow keys and pressing Select button or by pressing the button located below the screen under the Select Results Displays the results of the previous four tests Scroll up or down to view additional results CHNS Units PPh ppb Min ppb 2 2 Max ppb 200 200 Decimals a Press 4 gt or SELECT to edit value meae Caneel Units Determines units for displaying metal concentration You can change this to ppb ppm uM or nM Min ppb At concentrations less then min ppb Below Limit is displayed Max ppb A
15. mple cell compartments Do not insert a brush or sharp object into sample cell compartment to avoid damaging the mechanical components Dry the cleaned parts carefully with a soft cotton cloth Display Take care not to scratch the display Do not touch the screen with ballpoint pens pencils or similar pointed objects Clean the display with a soft lint free and oil free cotton cloth Diluted window cleaner liquid can also be used Sample Chamber Splashes or spills on and in the instrument should be cleaned up immediately Remove any liquid inside the sample chamber by using a non lint swab Do not tip the instrument to empty liquid from the sample chamber Sensors Never reuse sensors Once the sensor bag is exposed to air it should be used immediately within 30 minutes as exposure to humidity air and or heat can affect its effectiveness Dispose used sensor and all other used consumables such as sample tubes syringes plastic pipettes after each analysis Pipettes The 100uL automatic pipette can be reused as it uses disposable pipette tips Pipette tips should be disposed of immediately after use ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 13 of 17 AND1000 Fluorimeter for Water Testing 7 Customer Service Contact Information Contact us by Email info andalyze com By Telephone 888 388 0818 Toll Free in US or 1 857
16. nary Labels Read all labels and tags attached to the instrument Personal injury or damage to the instrument could occur if not observed A symbol if noted on the instrument will be included with a danger or caution statement in the manual This symbol if noted on the instrument references the instruction manual for operation and or safety information Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after 12 August of 2005 In conformity with European local and national regulations EU Directive 2002 96 EC European electrical equipment users must now return old or end of life equipment to the manufacturer for disposal at no charge to the user Note For return for recycling please contact the equipment producer or supplier for instructions on how to return end of life equipment producer supplied electrical accessories and all auxiliary items for proper disposal ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 3 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 2 3 Chemical and Biological Safety DANGER Potential Chemical Biological Exposure Hazards Handling chemical samples standards and reagents can be dangerous Users of this product are advised to familiarize themselves with safety procedures and the correct use of chemicals and to carefully read all relevant Material Safety Data Sheets
17. or industrial water contact ANDalyze customer service for additional application notes Water Sampling For best results use freshly collected sample unpreserved for analysis We recommend that you use the sample within 1 hour maximum of 2 hours of collection to minimize any metal loss to the walls of the sample container This is particularly important for testing trace lead levels Once the sample is mixed with ANDalyze sample buffer test within 15 minutes pH Range Tests have shown that environmental samples preserved in acid to a pH lt 2 cannot usually be brought to an appropriate pH when mixed with the ANDalyze buffer These samples must be first neutralized with NaOH to a suitable pH before mixing with ANDalyze buffer Please contact ANDalyze customer service for instructions related to pre treatment of highly acidic or highly basic samples 4 Set Up and Operating Procedures 4 1 Charging the Battery Important Before using the instrument assure that the fluorimeter device is charged To Confirm the Battery Level 1 Charging the Battery 1 2 Turn Power ON by pressing and Holding the ON OFF button See Horizontal Arrow on Photo to Right Once device is initiated confirm the battery pheno 1029 ie Press SELICT to tA indicator status is green See Vertical Arrow on Photo to Right The battery indicator should not be red as seen in the photo to right Release the Rubber Boot at the bottom of the meter
18. r methods will accompany the sensor in the kit 3 4 Operating Environment and Storage Important The following conditions are necessary to ensure correct instrument operation and accurate results Place the instrument firmly on a flat and even surface during operation Maintain an ambient temperature of 10 to 40 2C 50 to 1042F for proper fluorimeter operation Please see the manual for each specific metal for sensor specific temperature conditions The relative humidity should be less than 80 moisture should not condense on the instrument Do not operate or store the instrument in extremely dusty damp or wet locations Keep the surface of the instrument the cell compartment and all accessories clean and dry Sensors are stable up to 1 year if stored at lt 23 C 74 F lt 50 R H away from direct sunlight For best results store in the refrigerator 4 C 39 F Buffer solutions are stable up to 6 months if stored at lt 23 C 74 F but if stored in the refrigerator 4 C 39 F can be used for up to 1 year Sensors and buffers must be brought to room temperature before use ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 5 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 3 5 Water Testing Guidelines Note This is a drinking water test kit If you are testing other matrices such as surface water ground water
19. selecting that site in the future this calibration can be used repeatedly Please see the specific Testing and Calibration Manual for detailed instructions To create a new site 1 Scroll to the Site field 2 Using the up down keys 3 Enter the name of the and press Select select New Site location 8 characters max and press Save nter up to 8 characters 123 ABCDEFG Sensor Site None 456 HIJKLHN 789 BPQRSTU CIVHXYZ save Cancel Time 16 84 14 55 Press SELECT to Edit 4 fSensor Lot option Eitan o dioit ot tuner If the Temperature is turned ON you al option is turned Please enter the will have the option 123 ON you will have temperature of the sample water to enter the lot of 456 the option to enter the sensors 789 the temperature of 12 F BEE the sample water Press 4b to change value ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 11 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 5 2 On Site Calibration When testing water at a new location the instrument has to be calibrated for accurate readings Completing the On Site Calibration requires two separate solutions and tests At the end of the process the fluorimeter is calibrated for testing water at that site and also provides the lead test results for this site A calibration may only be accurate when origin
20. t concentrations greater than max ppb Above Max is displayed Decimals Changes the number of decimals displayed in the results Under some circumstances the decimals will be defaulted Note The Default values are the settings recommended by the manufacturer 5 6 7 About About Date Time Help Hardware 2 6 Firmware 2 1 6 Updated 9 11 12 Hardware 16 Firmware 18 Bone Displays the Hardware and Firmware versions of the meter including the most recent date the firmware was updated Note Update firmware if it has not been done recently Date and Time Set the date and time to maintain accurate history Edit using the arrow keys and Save ume r ae A 11 53 43 Displays How To video showing a sample calibration or provides link to view videos online ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 8 of 17 AND1000 Fluorimeter for Water Testing Options Power Save This sets the minutes when the display will turn black to save power Settable from 1 to 5 minutes Power Off This is the minutes which the unit will turn itself off after it goes into power save mode Settable from 6 to 15 minutes Cuvette Removal Turning this ON will cause a screen to appear after each test reminding the user to remove the cuvette from the sample chamber Test Instructions In the ON position 4 step instr
21. terial nonconformity in the Fluorimeter Device will be at ANDalyze s option and discretion to repair or replace the Fluorimeter Device at ANDalyze s expense or to refund the purchase price but not any taxes export or shipping fees and subject to the limitations in The foregoing remedy is subject to the Limitation of Liability in Section 15 of these terms To qualify you must notify ANDalyze during the Warranty Period of any problems that you experience with the Fluorimeter Device ANDalyze will have no liability for any nonconformity of which you fail to notify ANDalyze prior to the expiration of the Warranty Period This warranty does not apply to i Fluorimeter Device which has been used in a manner other than as authorized under these Terms and the documentation provided with the Fluorimeter Device including the product brochure and fluorimeter specifications ii any software on the Fluorimeter Device that has been modified by you or any party other than ANDalyze or which has been improperly installed to the extent such modification or improper installation caused the breach of warranty iii failures caused by accident neglect failure to maintain a suitable operating environment tampering or any other event other than ordinary use ANDalyze Inc 800 Boylston Street Prudential Tower Floor 16 Boston MA 02199 USA Tel 1 857 453 6740 www andalyze com Page 16 of 17 AND1000 Fluorimeter for Water Testing October 1 2012 ANDalyz
22. uctions will display in order when Test is selected from the home screen After the 4 step the test will begin In the OFF position the testing begins immediately Step 1 of 4 Fill the sample tube with sample water to the 5mL mark and mix LOAM Advanced User can View and Set parameters for each specific sensor Edit values using up down keys and SELECT Window Determines the number of points that will be used to calculate the rate of fluorescence increase over time We use a moving average method to calculate maximum rate If the value of window and samples is same then the average slope is calculated Samples Total data points taken which determines time up to which data is collected A value of 100 is approximately 20 seconds Increase sample value to increase data collection time Generally an increase in samples can increase sensitivity especially in matrices such as waste water October 1 2012 Power Save minutes 2 Power Off minutes Cuvette Removal Test Instructions ON Sensor Lot Temperature OF F Temperature Units F Display Contrast 6 Press 4 gt to change value Sensor Lot Turning this option ON allows the user to enter the lot number of the sensors at the time of a site calibration This helps to identify when a new site calibration is required new lot batch Temperature Turning this option ON allows the user to enter the temperature of the sample water at the

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