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UNICORN™ DAQ 1.0 - GE Healthcare Life Sciences

1. Created Most recent creation dates first 88 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 5 Files and folders in UNICORN DAQ 5 3 How to arrange and locate your files How to change the sorting order Select one of the methods below to change the sorting order e Select View Sort and the option that you want or e Right click and select Sort and the option that you want from the short cut menu or e Click the column header for the option that you want to sort by a second click on the same header will reverse the order Note Only the currently active window is affected How to find files The table below describes how to perform a search for files Step Action 1 Click the Results window and e Select the File Find menu command or e Right click and select Find from the shortcut menu Result The Find files dialog box opens x Path JC default Found folders and files e Name Path Name Verification fed batch 20L bag001 Z h Verfication fed batch 20 CA defaut Type Result fies z F Include subfolders F Dae from s000 y to 2000728 v File content Batch ID M Find Close Help lt gt l UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 89 5 Files and folders in UNICORN DAQ 5 3 How to arrange and locate your files 90 Step Action 2 Add search criteria to the dialog box for example Type a name in
2. 1 e Click the Windows Start button and e Choose the Control Panel 2 Click the Systems and Security heading 3 Under the heading Action Center click the Change User Account Control settings option GO E Control Pand s Sytem and Secunty Ub Control Panel Home x Y Action Center System and Security Review your computer s status and resolve issues Change User Account Control settings Troubleshoot common computer problems Network and Internet Restore your computer to an earlier a Hardware and Sound Windows Firewall Programs Check firewall status Allow a program through Windows Firewall 4 4 Search Control Pane User Accounts and Family Safety Ba System Appesraniceand View amount of RAM and processor speed Check the Windows Experience Index Personalization Allow remote access Seethename of this computer Device Manager Clock Language and Region amp Windows Update Ease of Access Turn automatic updating on or off Checkfor updates View installed updates Power Options Require a password when the computer wakes Change what the power buttons do Change when the computer sleeps p Backup and Restore D Backup your computer Restore files from backup ky Windows Anytime Upgrade Get more features with a new edition of Windows 7 A Administrative Tools KA Free up diskspace Defragment your hard drive Create and format hard disk partitions View event logs Schedule
3. Adjust to right margin Adjusts the selected object s to the right margin Adjust to center Adjusts the selected object s to the center of the page Make same size Adjusts the selected objects to the same size as the highlighted reference object Make same width Adjusts the selected objects to the same width as the highlighted reference object Make same height Adjusts the selected objects to the same height as the highlighted reference object Note The Make same size and Make same width functions can only be used to resize the width of chromatograms free text and picture objects UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 163 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report How to print the report The table below describes how to print the report Step Action oh e Choose File Print or e Click the Print icon Result The Print dialog box opens Note Printers are set up in the File menu of the UNICORN Man ager e Select the printing range e Select the number of copies Click OK Note You can also print the report from the Generate Report dialog box How to save the report in PDF The table below describes how to save the finished report as a PDF file Action format Step 1 2 164 e Click the UNICORN Manager icon on the Windows taskbar Result The UNICORN Mana
4. Step Action i Right click the Curves pane and select Marker 2 Drag the marker line with the mouse Result Where the line bisects the curve the X axis and Y axis values are displayed at the top right corner of the pane Note Right click and select Snapshot to record the marker position values See Section 2 2 6 Snapshots on page 32 for more information about the Snapshot function How to set a reference point When the vertical marker is displayed you can set a reference point to display curve data The table describes how to set a reference point Step Action 1 e Display a Marker in the Curves pane e Right click and select Set Marker Ref Point to define a reference point for the marker position 2 When the marker is moved from the reference point the X axis and Y axis values for the new position are displayed together with e the new position in relation to the position of the reference point e the minimum maximum and average values for the curve interval be tween the reference point and the new position How to change the curve colors and styles The Curves pane displays graphs for the selected curves in different colors with any reference curves included with the method as dashed lines The table below describes how to change the curve colors and styles 106 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 2 How
5. UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 119 6 How to monitor and acquire data from cell cultivation runs 6 4 If the network connection fails 6 4 Ifthe network connection fails Results will be saved in the Failed folder If the results of a data aquisition are stored on a server or other location and there is a network communication failure during a data acquisition that has been started from a remote station the data acquisition will continue and the results will be saved in the Failed folder on the local station A control mode connection can be established on the local station to control the data acquisition 120 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 How to view results Introduction A result file containing the acquired data from a cell cultivation can be generated at the end of the data acquisition It will contain a complete record of the acquired data includ ing settings curve data compiled in chromatograms and a run log The Evaluation module offers facilities for presentation and evaluation of the curve data This chapter describes how to present the chromatograms and curves of your result file and how to create and print reports In this chapter This chapter contains these sections Section See page 7 1 How to open a result file 122 7 2 How to use the File Navigator 124 7 3 Basic presentation of chromatograms 130 7 4 How to opti
6. 7 How to view results 7 1 How to open a result file e Do the following Select View File Navigator Locate and select a result file from the File Navigator Note See Section 7 2 How to use the File Navigator on page 124 for detailed instructions on how to locate files and set up File Navigator preferences UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 123 7 How to view results 7 2 How to use the File Navigator 7 2 How to use the File Navigator Introduction The File Navigator can be used to locate and open result files in the Evaluation module Recent runs are also listed based on the user preferences How to open the File Navigator To open the File Navigator e Click the Evaluation module icon in the Windows task bar e Select View File Navigator Result The File Navigator opens in the Evaluation module The File Navigator can be resized and dragged to other positions in the module 124 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 2 How to use the File Navigator How to open files from the Files list The table below describes how to use the Files list to locate and open a result file Step Action 1 e Click the Files tab Result The Files list opens in the File Navigator The list is identical to the Results window in the UNICORN Manager and shows all user available folders and files Recent Runs Files Find c 32003092
7. Table of Contents UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 5 1 Introducing UNICORN DAQ 1 Introducing UNICORN DAQ Introduction This chapter contains e Ageneral overview of the UNICORN DAQ software e nformation about the user documentation for UNICORN DAQ and how to use it In this chapter This chapter contains these sections Section See page 1 1 About UNICORN DAQ 8 1 2 About this manual 11 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 1 Introducing UNICORN DAQ 1 1 About UNICORN DAQ 1 1 About UNICORN DAQ Introduction This section is a general overview of the UNICORN DAQ system What is UNICORN DAQ UNICORN DAQ 1 0 for WAVE Bioreactor is a complete package for supervision of WAVE Bioreactor systems It also provides facilities for the evaluation of data from the cell culture It consists of software for acquisition and evaluation of data and data management It also includes a converter unit for interfacing the monitoring PC to the WAVE Bioreactor instrument Note that the UNICORN DAQ 1 0 for WAVE Bioreactor software is used to monitor the operation of the WAVE Bioreactor system and to collect result data but not to control the WAVE Bioreactor system The UNICORN software family includes specialized versions for a number of other appli cations apart from cell cultivation including liquid chromatography oligonucleotide synthesis and cross flow filtration U
8. Click the Browse button to select the folder where the backup file is lo cated In the Items field select which information to include from the backup file Click the Restore button to restore the system definitions Note You can Click the Information button to see which informa tion files are included in the backup file UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 81 4 General system operations 4 6 How to set up a printer 4 6 How to set up a printer Introduction UNICORN DAQ uses the default printer and printer settings that are installed on your computer You can change your printer by changing the default Windows settings but you can also set up a printer in UNICORN DAQ for the current working session How to set up a printer The table below describes how to set up a printer in UNICORN DAQ Step Action 1 Select the File Printer Setup menu command in the UNICORN Manager module Result The Print Setup dialog box opens Select a printer from the Name drop down box Change all printer Properties as necessary Change Paper and Orientation settings as necessary If you wish to access a network printer that is not shown in the Name drop down box e Click the Network button Result The Connect to Printer dialog box opens e Locate and select the network printer by browsing the network in the Shared printers field e Click OK to select the printer and close the Connect to
9. If some of these curves are not be needed anymore select Edit Delete Curves in the Evaluation module to remove the curves Note The original curves that were created during the run can never be deleted How to save the results 184 You can either save your edited results in the original file or in a new result file The table below describes how to save the results in the Evaluation module If youwanttosavethe then edited results in the original result file select File Save or e click the Save toolbar icon ial in a new result file select File Save as Note The previous version of the result file will be overwritten if you save the changes This cannot be reversed However the raw data curves remain unchanged UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 8 How to edit results 8 5 How to save results and exit the Evaluation module How to exit the Evaluation module The table below describes how to exit the Evaluation module Step Action I Choose File Exit Result If there are unsaved changes a dialog box opens with an option to save the changes before exit 2 Select Yes if you want to save the changes Result The result file is closed in the Evaluation module and the UNICORN Manager module is displayed UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 185 9 System settings 9 System settings Introduction This chapter descri
10. Result The preformatted report is displayed in the Evaluation module 7 Click the Print icon Li Result The report is printed UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 Installation Introduction This chapter describes how to install e the external Converter 100 controller unit for WAVE Bioreactor systems e the UNICORN DAQ 1 0 for WAVE Bioreactor software In this chapter This chapter contains these sections Section See page 3 1 How to install the Converter 100 40 3 2 Software installation and system definition 45 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 39 3 Installation 3 1 How to install the Converter 100 3 1 How to install the Converter 100 Introduction The Converter 100 controller unit provides an interface between the WAVE Bioreactor system and the UNICORN DAQ computer where the data from the ongoing cell cultivation is acquired It features an internal CU 960 controller unit with a Profibus interface a standardized and efficient serial fieldbus which is optimized for process automation applications The CU 960 is connected to two internal Modbus to Profibus adapter units each with a special application for WAVE Bioreactor instruments How to connect the Converter 100 The table below describes how to connect the Converter 100 Tip The Converter 100 connections are described further in the Converter 100 Instructions which i
11. To remove a file select the checkbox and click the Delete button UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 197 10 Error reporting 10 1 How to generate problem reports 10 1 2 How to generate a problem report from the System Control Step Action 3 To include more information in the report select the appropriate check boxes in the System information field By default all options are checked Computer amp operating system information A summary of the computer and operating system information for example type of processor processor speed RAM hard disk capacity and printer Integrity check When UNICORN is installed a checksum calculation is performed on the stationary files dll and exe for the system An integrity check means that a new checksum calculation is performed for the same files in their folders This new calculated value is compared with the checksum value obtained during installation The results of the comparison are presented in the report and any deviations are included Click the Next button Result The Generate report dialog box is displayed Go to step 3 below Step 3 How to generate and save The table below describes how to generate and save the report Action By default the report is saved in the folder Unicorn Reports If you want to save the report in another location select a folder in the tree structure You also have these options e Click th
12. message dialog box The Generate Report Wizard can also be activated anytime if you choose System Report Step 1 How to create the report When an error message appears in System Control follow the instructions in this table to activate the Generate Report Wizard and create a report Step Action 1 e Click the Report button in the error message dialog box t a message and push Help or F1 and the CUS50 Fir Try restarting the computer Enter the Main Menu Administration System Setup dialogue Edit the system with connection problems Check that the Strategy the Computer name and the Control unit number is correct according to installation at the local station If persistent try restarting the computer the system and the CU K CN or e Choose System Report 2 The first step is a Welcome screen Click the Next button Result The Description dialog box is displayed and shows a list of the problems errors that have occurred All the problems errors that have oc curred together with help texts are automatically recorded and included in the report Tip If you select a specific error in the Description dialog box the appropriate help text is shown in the error message box UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 195 10 Error reporting 10 1 How to generate problem reports 10 1 2 How to generate a problem report from the System Control Step Action 3 Add
13. 16 38 15 User default at computer SEHC88YDP2JL connected to system Wave MethodQueue 03 26 2010 16 56 00 User default at SEHC88YDP2JL logged off 03 26 2010 16 56 00 User default at computer SEHC88YDP2JL disconnected from system Wave Maintenance L 03 30 2010 14 52 06 User default at computer SEHC88YDP2IL is in Control mode of system Wave Information Timezone The items of the Global tab The table below describes the items which can be displayed on the Global tab UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA A Audit trails Item Displays User e user creation deletion and redefinition e creation of rescue file for system definitions e restoration of system definitions with rescue file e changes of global and personal lists report format templates Backup backup operations for global audit trail files Logon Logoff e all logon and logoff attempts e the name of the user logging on or off e failed logon attempts Define system system definition deletion and definition Maintenance e maintenance activities that are scheduled in the Sys tem Control module menu command System Main tenance e settings made in maintenance module Information e system lock unlock e socket on off e global log started stopped Timezone the time zone where the activity was logged UNICORN DAQ 1 0 User Manual for WAV
14. Printer dialog box Click OK in the Print Setup dialog box to complete the setup Note To save created reports electronically you can select to print the files in PDF format To be able to do this you must have a full version of Adobe Acrobat installed and select PDF Writer or Distiller in the Printer Setup Other compatible PDF software will serve the same purpose 82 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 5 Files and folders in UNICORN DAQ 5 Files and folders in UNICORN DAQ Introduction All UNICORN DAQ data is organized in files and folders Files and folders are handled like in any other Windows application with some exceptions This chapter describes how to work with UNICORN DAQ files and folders with the focus on the topics that are specific for UNICORN DAQ In this chapter This chapter contains these sections Section See page 5 1 How to create folders 84 5 2 How to open and preview files 85 5 3 How to arrange and locate your files 88 5 4 How to copy delete rename and backup files and folders 91 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 83 5 Files and folders in UNICORN DAQ 5 1 How to create folders 5 1 Howto create folders Introduction This section describes how folders are organized in UNICORN DAQ and how to create a new user specific folder for the user s results UNICORN folders The files and folders are displayed in
15. Report Format dialog box opens 4 e Select the Customized format and click OK Result The Report Editor opens in Edit mode UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 151 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report The Edit mode window The illustration below shows the Report Editor window in Edit mode with a blank report open Popes bot Cae Mode Toolbar button functions in the Report Editor The table below describes the different functions of the Edit mode toolbar buttons in the Report Editor Toolbar button Function Preview Edit This button toggles between a print preview of the report and the Edit mode Next Page This button displays the next page or pair of pages where there are more than one page Prev Page This button displays the previous page or pair of pages where there are more than one page One Page Two Pages This button toggles between single page view and pairs of pages view when there is more than one page Zoom In This button increases the magnification of the view 152 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report Toolbar button Function Zoom Out This button decreases the magnification of the view Add Page This button
16. Run Data pane Step Action 2 Select the Run Data Groups tab and if desirable do one or more of the fol lowing e Select an available group to be displayed in the list to the left e Edit an available group Select the group from the list on the left and click the Edit Group button Modify the included readings in the list to the right and click OK e Create anew group Click the New group button and select the readings that you want to view from the list Enter a name for the group and click OK e Delete a group Click the Delete Group button and select a group in the Delete Layout dialog box click OK and confirm the deletion 3 Select the run data parameters that you want to display in the list to the right 4 Click OK to view the selected items in the Run Data pane The name of the selected layout replaces the default layout name Run Data How to change text color or text background 104 The table describes how to change the text color or background in the displayed reading boxes Step Action i Right click on the pane and select Properties Result The Properties dialog box is displayed 2 Select the Run Data Color tab 3 Click the Text or Background buttons Select a new color and click OK Result The color change is displayed in the test field 4 Make further adjustments to the colors as appropriate 5 Click OK to apply the changes UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9
17. affect the report and not the view of the chromatograms in the Evaluation module 5 Click OK Result The chromatogram is inserted onto the page How to include a method The table below describes how to include a method in the report Step Action 1 e Click the Method icon e Press and hold the left mouse button on the report page and drag out a box to the size of the item Release the button Result The Setup Method dialog box opens 2 Select the items to be included in the report e Main Method is the method on which the run was based e Blocks are the blocks that were used in the method UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 159 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report Step Action 3 e Select the appropriate Settings Note Expand main displays the expanded method view e If desired change the Fonts e Click OK Result The method object is inserted onto the page How to add documentation The table below describes how to add documentation to the report Step Action 1 e Click the Documentation icon E e Press and hold the left mouse button on the report page and drag out a box to the size of the item Release the button Result The Setup Documentation dialog box opens 2 Select the items to be included in the report e Select All includes all items in the report e Clear All removes a
18. and Logbook panes displayed Q System Control 1 wave Method default Metod1 m02 Result C default Fed batch 50t laj Run Ena ES Run Data Instruments Connection Run Status Acc Time Block Time Rate Angle L Airflow L Temp LDO 8 E A Ce ee E Ee E E E zi en eee 4 3 2 45 9 8 B 0 BN 8 3s ra G h 38800 386802 368 04 36806 386 08 36810 36812 36814 368 16 36818 368 20 368 22 38824 36826 368 28 36830 36832 36834 38836 36838 38840 36842 368 44 Angle 6 a s eo Speed rpm 20 1 sp 200 Perfusion on Weight kg 25 63 sp s0 00 m 2803h Lef Bag 02 Deviation Logbook a 368 09 h lt ACK gt default Left Bag 02 Deviation 368 10 h Left Bag 02 Deviation 368 11 h Set_Mark New DO set point chosen Manual 368 11 h lt ACK gt default Left Bag 02 Deviation 368 14 h Set_Mark Decreased DO control cycle time Manual 368 19 h Set_Mark Increased feeding pump speed to 50 rpm Manual R 368 55 h Set_ Mark feeding terminated Manual F For Help press F1 Run Man No watch Controlled By default UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 101 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 1 How to customize System Control panes How to select what panes to display Each System Control mod
19. boxes in the chromatogram The list below describes how to edit the text UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 173 8 How to edit results 8 1 How to enter and edit text in the chromatogram e Click the text and type the new text e Click outside the text box to set the text 174 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 8 How to edit results 8 2 How to rename chromatograms and curves 8 2 Howto rename chromatograms and curves Instruction The table below describes how to rename chromatograms or curves in the Evaluation module Step Action 1 Choose Edit Rename and the relevant option Chromatogram or Curve Result The Rename dialog box opens 2 Select the appropriate object 3 Type a new name in the Name field 4 Click OK Note The original raw data curves cannot be renamed They will not be listed as options in the dialog box UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 175 8 How to edit results 8 3 How to import and export results 8 3 How to import and export results Introduction Curves and data can be imported and exported in different formats This section describes how to import and export results In this section This section contains these topics Section See page 8 3 1 How to import results 177 8 3 2 How to export results 178 176 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 8 How to edit res
20. confirmation box opens 2 Click Yes to confirm that you want to quit 3 A Warning opens if you have any unsaved data in the Evaluation module e Click Yes to continue to close the program Your unsaved data will be lost when the program is closed e Click No to return to the program and save your data Note Do not shut down Windows or turn off the computer if you quit UNI CORN DAQ while a cell cultivation run is in progress In case your access to the UNICORN Manager is restricted you will still be able to quit the program UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 71 4 General system operations 4 2 How to create a new user 4 2 How to create a new user Introduction This section describes how to create a new user and assign a home folder for the user s methods and results Default user A default user is created when the system is installed The default user has unrestricted access to all UNICORN functions You log on with this profile when you access a newly installed system for the first time The table below describes how to log on as the default user Step Action i Select user default from the user drop list 2 Type password default if necessary Note The default user is the only user that is allowed to use the user name as password 3 Click OK or press the Enter key q UNICORN logon User name default i Password OOOO Cancel Hep Note We r
21. curves 8 How to edit results 8 3 How to import and export results 8 3 2 How to export results The table below describes how to export curves in the Evaluation module Step Action 1 Choose File Export Curves Result The Export Curves dialog box opens Export Curves Source chromatogram Curves to export wi 09 Fed batch 50L verification001 11_L_02 04 Fed batch 50L verification001 11_F_Airtlow 05 Fed batch 50L verification001 11_L_Temp 06 Fed batch 50L verification001 11_R_Temp 07 Fed batch 50L verification001 11_L_CO2 08 Fed batch 50L verification001 11_R_CO2 09 Fed batch 50L verification001 11_L 10 Fed batch 50L verification001 13 Fed batch 50L verificationO01 11_L_pH 114 Fed batch 50L verificationO01 11_R_pH 115 Fed batch 50L verification001 11_L_DO o 10 Fed batch 50L verification001 11_R_02 013 Fed batch 50L verification001 11_L_pH 14 Fed batch 50L verification001 11_R_pH Cut curves From Reduce number of samples 5 Se Normalise retention Reduce by factor 33 Max no samples 990 Export X Cancel Help 2 e Select the curvels you want to export e Enter parameters to limit the curvel s if necessary e Click the Select button e Repeat Step 2 to select more curves 3 Click the Export button Result The Export Curves to File dialog box opens 4 Select the e
22. modules e Show unused variables Show variables that are not used in the method on the Variable page of the Start Protocol Not applicable for UNICORN DAQ e Show variable details Show detailed method variables on the Variable page of the Start Pro tocol Not applicable for UNICORN DAQ UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 3 How to change your passwords and user attributes Step Action 3 Mark heights Select a size definition and type the height for the following marks e Logbook mark 4 Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 77 4 General system operations 4 4 How to connect to the WAVE Bioreactor 44 Howto connect to the WAVE Bioreactor Introduction A computer can have up to four WAVE Bioreactor systems connected at a time This section describes how to connect to the systems and different connection modes How to establish a connection The table below describes how to connect a WAVE Bioreactor system that is locally connected to your computer Step Action 1 Open a System Control module Note Each UNICORN installation may have up to four System Control modules The number of modules are selected when the software is installed 2 e Select the System Connect menu command or e Click the Connect to system toolbar icon rA Result The System Connect dialog box opens 3 Select the system you
23. option to let UNICORN DAQ add a unique identifier number to your result name You can use the same name for a series of runs with each in dividual result designated by the identifier number You can choose the option of performing data acquisition without generating a result file Tip When the data acquisition is in progress you may want to create a reference curve using data that you enter manually This is described in Section 6 3 2 Manual instructions on page 116 98 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 1 How to start the cell cultivation data acquisition Notes The Start notes will be shown and editable at the method start It is advisable to add relevant data about the cell cultivation here such as cell type culture media batch numbers and calibration regulator parameters The notes can also be used during run and evaluation Run notes and Evaluation notes but only Evaluation notes can be edited after the run has ended Notes are not automatically added as Logbook entries To create entries that will be registered in the Logbook you must use the Set_Mark instruction This is described in How to enter a Set_Mark instruction on page 117 How to finish the data acquisition Press the End button to finish the data acquisition A dialog will open asking if you will save a partial result from the data acquisition It is essential
24. options in the dialog box e Click the Export button e Select a destination folder and type a file name 180 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 8 How to edit results 8 3 How to import and export results 8 3 2 How to export results Copy to the clipboard You can also use the Windows clipboard to copy the contents of the active window and paste it into other programs e g Microsoft Word Curves and documentation are copied as Windows enhanced metafiles emf and peak tables are copied as text Only the peak table columns that are selected in the spreadsheet will be copied UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 181 8 How to edit results 8 4 How to sign results electronically 8 4 How to sign results electronically Instruction Result files can be signed electronically to enhance data file security The table below describes how to sign a result file electronically in the Evaluation module Step Action 1 Choose File Sign Result Result The Sign the Result dialog box opens Sign the Result D default Fed batch 50L verification001 E3 Signing View Signatures Sign as user User Password Full name Position Signature Meaning Password gejaut default default Result file reviewed xa M Lock 182 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 8 How
25. problem reports This section describes how to generate a problem report from the UNICORN Manager Step 1 How to create the report The table below describes how to create a report with the Generate Report Wizard Step Action 1 Select Administration Create System Report in the UNICORN Manager module The first step is a Welcome screen e Click the Next button Result The Systems dialog box opens with a list of the available systems for the logged on user Select a system for which the report is to be generated and click the Next button Result The Description dialog box opens Add the following information in the dialog box e a short description of the problem e the circumstances under which the problem occurs e the consequences of the problem Click the Next button Result The Reproducibility dialog box opens UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 191 10 Error reporting 10 1 How to generate problem reports 10 1 1 How to generate a problem report from the UNICORN Manager Step Action 5 Specify whether the problem is reproducible or not Select one of these al ternatives e Yes Provide a short description in the text box of how the problem can be reproduced e No e Unknown Click the Next button to proceed to attach example files see table below Step 2 How to attach a file You can attach result files and log files to the problem report The table below d
26. right click in the pane and select Stretch in the shortcut menu 110 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 5 The Logbook pane 6 2 5 The Logbook pane Introduction All actions including method start and end manual interventions such as Set mark or External value and unexpected conditions such as warnings are logged for every run with date time and current user name where appropriate The logbook thus provides a complete history of any given run The log is saved in the result file Note Information added in Run Notes are not included in the Logbook To be included in the Logbook text should be entered as Set marks This is described in How to enter a Set_Mark instruction on page 117 Illustration The illustration below shows an example of the Logbook pane 368 09 h Left Bag 02 Deviation 368 09 h lt ACK gt default Left Bag O2 Deviation 366 10 h Left Bag O2 Deviation 368 11 h Set_Mark New DO set point chosen Manual 366 11 h lt ACK gt default Left Bag 02 Deviation 368 14 h Set_Mark Decreased DO control cycle time Manual 368 19 h Set_Mark Increased feeding pump speed to 50 rpm Manual 366 55 h Set_Mark feeding terminated Manual Autoscroll The Logbook pane can autoscroll to display the latest entries Right click in the pane and select
27. section if necessary Click OK About passwords The list below is a summary of facts and advice about UNICORN DAQ passwords The system can be set up to operate without required passwords The minimum number of password characters is set up at installation Passwords can be any combination of letters and numbers Passwords are case sensitive Avoid using obvious passwords e g your username your telephone number etc The settings in the User properties determine the expiration for a password Change passwords regularly even if your user profile is set up without password expiration UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 75 4 General system operations 4 3 How to change your passwords and user attributes How to change user attributes 76 The table below describes how to change your user attributes Some items that are not applicable for UNICORN DAQ are grayed out and cannot be selected Action Select Administration Change User Attributes Result The Change user attributes dialog box opens Change User Attributes Use large toolbar icons C Show unused variables C Show variable details Jefault overwrite of t Quick view cuve 1 Fraction mark height Injection mark height l Logbook mark height 3 Character heights v C Dialog check box options The dialog check box options are described below e Use large toolbar icons Display large toolbar icons in all
28. the following information in the Description dialog box e A short description of the problem e The circumstances under which the problem occurs e The consequences of the problem Click the Next button Result The Reproducibility dialog box opens Specify whether the problem is reproducible or not Select one of these al ternatives e Yes Provide a short description in the text box of how the problem can be reproduced e No e Unknown Click the Next button to proceed to attach example files see table below Step 2 How to attach a file 196 You can attach log files to the problem report The table below describes how to attach a file UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 10 Error reporting 10 1 How to generate problem reports 10 1 2 How to generate a problem report from the System Control Step Action 1 The Attachments dialog box is displayed Attachments Result Method System log Global log Remove System information V Computer amp operating system information AKTA hardware informatior Integrity check lt Back Net Cancel Help 2 e Depending on the character of the file to be attached select the appro priate tab Result System log or Global log e Attach a file Click the Add button Selecta file in the dialog box and click the Attach or OK button Result The selected file is added to the tab in the Attachments dialog box Note
29. to display the menu shown in the picture below Optimizing the workspace The chromatogram window can be minimized and maximized using ordinary Windows commands The table below describes extra features to optimize the workspace Use the command if you want Window Arrange icons to arrange icons of minimized windows Window Tile to view several chromatogram windows side by side Window Cascade to stack the open windows like a deck of cards How to display a vertical marker line The table below describes how to display a vertical marker line Step Action 1 Right click the Curves pane and select Marker 2 Drag the marker line with the mouse Result Where the line bisects the curve the X axis and Y axis values are displayed at the top right corner of the pane UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 133 7 How to view results 7 3 Basic presentation of chromatograms 7 3 2 The chromatogram window Note Right click and select Snapshot to record the marker position values See Sec tion 2 2 6 Snapshots on page 32 for more information about the Snapshot function How to set a reference point The table describes how to set a reference point Step Action 1 e Display a Marker in the Curves pane e Right click and select Set Marker Ref Point to define a reference point for the marker position 2 When the marker is moved from the reference point the X axis an
30. to edit results 8 4 How to sign results electronically Step Action The Sign as user field shows the properties for the current user You can also choose another user from the droplist If you choose a new user the corresponding password must be typed in the Password text box Type a short text description for the signed operation in the Meaning field e g Result file reviewed The Lock check box is selected as default to lock the result file from further changes Type your signature password in the Password field and click OK Note You should only lock the result when you are sure that the result file will not be modified anymore Signatures associated with the result The View Signatures tab of the Sign the Result dialog box provides a list of all signatures associated with the current result The information on this tab is for viewing purposes only and cannot be changed UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 183 8 How to edit results 8 5 How to save results and exit the Evaluation module 8 5 Introduction How to save results and exit the Evaluation module After you have finished the evaluation process you can save all the changes you have made to the chromatograms including newly created curves and chromatograms that you have imported and created How to delete unwanted curves All the curves that you created during your manipulations will be saved in the chro matogram
31. to save the data at this point otherwise it will be lost For Help cress P Onn he I I nth Contrahed y dfa UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 99 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 Howtoset up UNICORN DAQ to monitor the cell culti vation Introduction This section describes how to set up the System Control to acquire data from the cell cultivation and how to customize the different panes In this section The table shows the topics that can be found in this section Section See page 6 2 1 How to customize System Control panes 101 6 2 2 The Run Data pane 103 6 2 3 The Curves pane 105 6 2 4 The Flow Scheme pane 110 6 2 5 The Logbook pane 111 100 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 1 How to customize System Control panes 6 2 1 How to customize System Control panes Introduction The System Control module displays the status of the current system On the Windows taskbar there may be up to four System Control modules available that can be connected to different systems Separate systems may be monitored independently of each other Illustration The illustration shows the System Control module with the Run Data Curves Flow scheme
32. to search in all result files or chromatograms UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 29 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 5 Help functions 2 2 5 Help functions Introduction There are different ways to get help and instructions in the UNICORN DAQ application e From the Help menu in each module e From the context sensitive help in each dialog box e By pressing the F1 key The Help menu The Help file 30 e By choosing the Help menu item from the Help menu in each module you can access the Help file e By choosing the Manuals menu item from the Help menu in each module you can access the installed manuals in PDF format The illustration below shows the Help menu of the UNICORN Manager module Help Help for UNICORN Manager Index p About UNICI The table below describes how to open and use the Help file Step Action 1 Choose Help index Result The Help file is displayed with the Index panel selected UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 5 Help functions Step Action 2 Search for topic in the Help Index Type a keyword you want help on in the text box in the panel Result The alphabetically closest matches are displayed in the list Select a match and click the Display button Result The associated help text is displayed in the rig
33. value is compared with the checksum value obtained during installation The results of the comparison are presented in the report and any deviations are included Click the Next button Result The Generate report dialog box is displayed Proceed to Step 3 How to generate and save the report below Step 3 How to generate and save the report The table below describes how to generate and save the report Step Action 1 By default the report is saved in the folder Unicorn Reports If you want to save the report at another location select a folder in the tree structure UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 193 10 Error reporting 10 1 How to generate problem reports 10 1 1 How to generate a problem report from the UNICORN Manager Step Action 2 You also have these options e Click the Preview button to open the report in Notepad e Click the Print button to print the report without any preview 3 Click the Finish button to generate and save the report 194 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 10 Error reporting 10 1 How to generate problem reports 10 1 2 How to generate a problem report from the System Control 10 12 Howto generate a problem report from the System Control Introduction The Generate Report Wizard is used to generate problem reports When an error mes sage appears in System Control you can activate the report wizard from the error
34. view mode connec tion System is available Any user can establish a connection UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 115 6 How to monitor and acquire data from cell cultivation runs 6 3 Manual instructions 6 3 2 Manual instructions 6 3 2 Manual instructions Introduction Manual instructions can be issued from the Manual menu in the System Control module Manual instructions are used to create curves based on external values or to enter text information in the chromatogram and Logbook This is described below External value curve instructions during a data acquisition Manual instructions can be used during a data acquisition run to enter external value instructions This will create a curve starting with the entered value The curve will con tinue at this value until a new value is entered manually The curve is then updated to reflect the new external value All manual instructions that you issue during a data ac quisition are recorded in the logbook The manual instructions dialog box The Manual menu in System Control opens a dialog box The name of the connected system is displayed on the title bar of the dialog box See an example in the illustration below System Other Instructions Instructions Parameters Set Mark Mark text Insert End_Timer O Curves Pause_Timer Execute Close Help C Auto update If this is checked the parameter fields will
35. want to connect 4 Click OK Remote connections Each computer workstation may have up to four WAVE Bioreactor systems connected locally In a network installation you may connect a system that is physically connected to another computer the local station Your system is then a remote station The local station that is connected to the WAVE Bioreactor system must be logged on to the network and the UNICORN drivers must be running However the connection will work even if the UNICORN program is not running on the local station 78 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 5 How to back up and restore system data 4 5 How to back up and restore system data Introduction You can create a backup file with system information and store it on a USB memory or another drive The backup file will contain information about e Global Files e Personal Files e System Files Afterwards you can use the backup file to restore the system definitions in case they are corrupted How to create a backup file The table below describes how to create a backup file and store it for example on a USB memory Step Action 1 Insert a USB memory into the computer if you want to store the backup file on a USB memory UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 79 4 General system operations 4 5 How to back up and restore system data Step Action 2 Choose Adminis
36. 0 User Manual for WAVE Bioreactor 28 9846 82 AA 207 B Data sampling Appendix B Data sampling Effective sampling frequency 208 The initial effective sampling frequency for each monitor is set in the system strategy It can be viewed and changed in the Curves group of System Settings in System Control The default initial sampling frequency for the sensors are set to sample at every 10th second After approximately 3 7 days anew chromatogram will automatically be started where the sampling will continue at the same rate A total of ten chromatograms will be created from the same acquisition run This will provide sampling at the same rate for up to 37 days If this time is exceeded the sampling rate will be halved in the last chromatogram only This is done by excluding every second sampling creating an effective samplig rate of every 20th second The sampling rate reduction will be repeated for the last chromatogram after the number of run days shown in the table below Maximum number of run days Effective sensor sampling rate Seconds between samplings 40 7 20 48 1 40 62 9 80 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA A Audit trails Global view 199 How to back up 206 How to print 205 How to renew 205 How to search for text 204 How to view 200 How to view older files 203 System view 199 Gs Chromatogram Layout Curve tab 137 Default curve names 137 How to c
37. 120 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 95 6 How to monitor and acquire data from cell cultivation runs 6 1 How to start the cell cultivation data acquisition 6 1 Howto start the cell cultivation data acquisition Before you start Before you start a data acquisition make sure that e the correct system is connected in control mode the correct system for the data acquisition is selected in the system selector of the System Control module e the system is prepared according to the system user manual How to start the data acquisition The table below describes how to start a method run from System Control Step Action 1 e Select File Instant Run or e click the Run button Note You can only use the Run button to start the data ac quisition if a run has been performed earlier on the system and you have not logged out since then Result The Instant Run dialog box is displayed For system Use l Wizard Template Template selection Method notes Use this method template to start a data acquisition en the cell cultivation is finished end the data Template acquisition and save the partial result by pressing END in System Control The data acquisition method will continue for 9999 hours 416 days if it is not ended manually Tip The Start notes will be shown and editable at method start It is advisable to add relevant data about the cell cultivation run her
38. 28 9846 82 AA 23 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 2 The System Control module Icon Function The Connect System icon is used to connect a WAVE PA Bioreactor system for data acquisition The Disconnect System icon is used to disconnect the Pt WAVE Bioreactor system a The Take Control of the System icon is used to leave the amp i view mode for the system and change into a control mode to perform manual instructions for the data collection The Leave Control of the System icon is used to leave the EA control mode for the system and change into a view mode 24 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 3 The Evaluation module 2 2 3 The Evaluation module Introduction The Evaluation module provides the necessary facilities to present and to evaluate curve data The module window Opened result files are displayed in the Evaluation module window See the illustration below Fed batch SOL verification001 11 Angle Fed batch 50L veiification00111 L Airflow FE Fad batch SOL verifioation001 11 L O2 Fed batch SOL verification001 11 L pH F Fed batch 50L verification001 11 Harvest Fed batch SOL verification001 11 Feed domm ee i IM i UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 25 2 UNIC
39. 4 320030925 920030930 320031001 320031002 20031007 320031016 20031017 B Example files manual runs System M4 Lui o02 lt 003 2 e Navigate to the desired folder e Double click the desired result file Result The result file opens in the Evaluation module UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 125 7 How to view results 7 2 How to use the File Navigator How to use Find to search for files 126 The Find function in the File Navigator is used to locate result files in the available folders The table below describes how to use the Find function to locate and open a result file Step Action 1 e Click the Find tab Result The Find list opens in the File Navigator x Recent Runs Files Find E 4 Result file name BE Value of variable Sample_ID i Example ResultOO1 c i Example Result002 c Example Result003 c i Fe xample Result004 fc R Type a file name or part of a file name in the Result file name text box Standard wildcard characters can be used M m mi Ot nt e Click the Quick Find button Result The located result files are listed in the File Navigator e Double click the desired result file or chromatogram Result The file or chromatogram opens in the Evaluation module Note Click the Find button to open the Find Files dialog box where more search functions are
40. 7 6 1 How to create and print a customized report Step Action 3 Click OK 166 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 6 How to create and print reports 7 6 2 How to edit an existing report format 7 6 2 How to edit an existing report format Introduction This section describes how to edit an existing report format How to edit a report The table below describes how to edit a report format in the Evaluation module Step Action 1 Open a result file 2 e Select File Report or e Click the Report icon Result The Generate Report dialog box opens 3 e Select a Customized Report Format to edit e Click the Edit button Result The report format opens in the Report Editor a e Double click the report item you want to edit e Make the desired changes in the dialog box e Continue to edit all items until the format is complete Note See Section 7 6 1 How to create and print a customized report on page 151 for more information 5 e Select File Save As Result The Save Report Format dialog box opens UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 167 7 How to view results 7 6 How to create and print reports 7 6 2 How to edit an existing report format Step Action 6 e Type a name in the Report format name text box Select the Save as global format check box to make the format available to other users Select the Save as d
41. 8 2 How to rename chromatograms and curves 175 8 3 How to import and export results 176 8 4 How to sign results electronically 182 8 5 How to save results and exit the Evaluation module 184 172 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 8 How to edit results 8 1 How to enter and edit text in the chromatogram 8 1 Howto enter and edit text in the chromatogram How to enter text Text can be added to the chromatogram The table below describes how to do this Step Action 1 e Right click the curves view of the chromatogram window and select Add text from the menu or e Choose Edit Text Add 2 Click where you want to insert text in the chromatogram Result A text box opens 3 Type the text 4 Click outside the text box to set the text How to edit the text The table below describes how to edit inserted text Step Action 1 Choose Edit Text Edit Result The Edit Texts tab of the Chromatogram Layout dialog box is dis played 2 e Select the text that you want to edit and make the appropriate changes in the Selected text field e Click the Change text button or the Delete text button e Use the Font and Set Orientation buttons if needed and make the de sired changes in the resulting dialog boxes 3 Click OK to apply the changes Shortcut option You can also right click outside the text box and select Edit Text Mode from the shortcut menu This activates all the text
42. 846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 3 The Curves pane 6 2 3 The Curves pane Introduction The Curves pane of the System Control module displays monitor signal values graphi cally The figure below shows an example of the Curves pane Rate L_Airflow L_Temp t_02 L_pH caren L_DO Net_Weight off set 751 Sa ARMAS RAMMS RAAUA RAGA RAKES RRR REDO OOOO OROES ROARS OARAR BAAS BRASS TTT h 00 05 1 0 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 How to select curves to be displayed You can decide which curves you wont to display in the Curves pane The table describes how to select the curves to be displayed on the screen Step Action 1 In System Control select View Properties Result The Properties dialog box is displayed 2 Select the Curves tab 3 In the Display curves list select the curves you want to display If you want all curves to be displayed click the Select All button If you do not want any curves to be displayed click the Clear All button 4 Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 105 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 3 The Curves pane How to display a vertical marker line The table below describes how to display a vertical marker line
43. A 7 How to view results 7 7 Run documentation Documentation Evaluation Log juestions Result Name TextMethod Notes Method Information Result Information Start Protocol Settings Calibration Logbook Information Signatures Run Summary Snapshots Result information Result name D default Fed batch 50L verification001 tes Batch ID F7E20DE0 B61D 4EAC BC67 5DA7FO43C30B Result created by default Date of creation 2010 07 28 10 39 00 W Europe Daylight Time Run on system Wave Result last modified by default Date of last modification 2010 07 28 10 39 00 W Europe Daylight Time Strategy information Strategy name Wave_20 50_ 118 Strategy date 2010 07 06 15 45 00 W Europe Daylight Time Strategy size 3859648 bytes Used Components Strategy Notes UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 171 8 How to edit results 8 How to edit results Introduction This chapter describes e how to edit the results that are presented in the Evaluation module e how to import and compare runs e how to import and export results For more information about how to view results see chapter Chapter 7 How to view re sults on page 121 In this chapter This chapter contains these sections Section See page 8 1 How to enter and edit text in the chromatogram 173
44. AA 17 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 1 UNICORN Manager Icon Function The System Control icon activates the first connected System Control module and displays the Manual instruction dialog box pe Note Ifno System Control module is connected a module will be activated and the System Connect dialog will be dis played The Evaluation icon opens the Open Result dialog box Select a result file 6 and click OK to start the Evaluation module Limited access to the UNICORN Manager Some user groups may be defined to have only a limited access to the UNICORN Man ager functions The available functions in the limited version are Log off e Change User Attributes e Change Password e Quit Program Help There is also a Cancel button which minimizes the dialog box The illustration below shows the limited access version of the UNICORN Manager UNICORN Manager UNICORN Logon Information Eric is logged on Change User Attributes Help Quit Program Change Password 18 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 1 UNICORN Manager Note For more information about how to change passwords and user at tributes please refer to Section 4 3 How to change your passwords and user attributes on page 75 For more information about how to log off and quit the program please refer to Sec
45. Autoscroll You can also select the Autoscroll option in the Properties dialog box View Properties and select the Logbook tab UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 111 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 5 The Logbook pane How to filter the logbook contents You can choose to display only selected items in the logbook The table below describes how to activate the filter Step Action 1 e Right click in the Logbook pane and choose Properties Result The Properties dialog box opens 2 e Choose the Logbook tab e Select the items you want to display in the logbook all items are selected by default e Click the OK button Result Only the selected items will be displayed in the logbook The Logbook title in the upper right corner will show the text Filter on to indicate that not all items are visible All items will still be logged in the result file How to find logbook text entries The logbook can be searched for specific text entries The table below describes the function Step Action 1 Right click in the Logbook pane and choose Find Result The Find dialog box opens 2 e Type the text you want to locate e Select search criteria if necessary e Click OK Result The located logbook entry is highlighted 112 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How
46. CORN DAQ System Control module during the test 42 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 1 How to install the Converter 100 Connecting several WAVE Bioreactor systems A UNICORN DAQ computer may have up to four WAVE Bioreactor systerns connected locally each with its own Converter 100 The Converter 100 can be connected via a standard network switch to a single UNICORN DAQ computer as shown in the example below Example The illustration shows three WAVEPOD II units each connected to a WAVE Bioreactor system Each of the WAVEPOD II units is connected to the primary port on a Converter 100 Also a WAVE Bioreactor 200 system is connected directly to the primary port of a Converter 100 The uplink ethernet ports on the converters are connected to the network switch The uplink port of the switch is connected to the network interface card of the UNICORN DAQ computer UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 43 3 Installation 3 1 How to install the Converter 100 Note By default the Converter 100 is set up to be the single or the first unit that is connected to the UNICORN DAQ computer with the ID number 1 This ID number also determines the series of TCP ports that are used for the communication The ID numbers and the TCP port numbers for all other converters connected via the network switch must be changed before they can be used Contact GE Healthcare services fo
47. Description 105 How to change curve colors and styles 106 How to change scale of the X axis 108 209 Index 210 How to change scale of the Y axis 107 How to display a vertical marker 106 How to select curves to be monitored 105 How to select text align ment 109 How to set a reference point 106 How to zoom in regions of the pane 108 Reduce scale of zoom 108 D Data acquisition runs If network communication fails 120 Data sampling Effective sampling frequen cy 208 Initial sampling frequen cy 208 Delete files and folders 93 Documentation Documentation tabs de scription 170 How to export 180 How to view 169 Result information 170 E Electronic signature How to sign a result 182 Evaluation Chromatogram window shortcut menu 133 Chromatogram window views 132 How to display a vertical marker 133 How to exit the module 185 How to make chro matogram layout changes general 136 How to optimize the chro matogram workspace 133 How to set a reference point 134 Evaluation logs How to export 180 F File Navigator How to change preference settings for Recent Runs 128 How to close 129 How to locate files from the Files list 125 How to open 124 How to open a recent run 127 How to open result files 85 How to use Find to locate a file 126 Files and folders Copy to external 92 How to copy 91 How to copy from exter nal 92 How to move 91 Flow Scheme pa
48. E Bioreactor and generate a printed report Note Step This assumes that a report format has been defined and saved If no report format is saved you will have to proceed to create the report manually in the Evaluation module This is described in Section 7 6 1 How to create and print a customized report on page 151 Action In the System Control module e Choose the File Instant Run menu command Result The Instant run dialog box opens e Select a system if several WAVE Bioreactor are available e Make sure that Template is selected e Choose the template method in the Template list e Click the Run button Result The Start Protocol opens e Enter information about the run in the Start Notes tab of the Notes page and click Next e Enter a result name in the Result Name page and click START Result The data acquisition begins and can be monitored in the System Control module Curves pane UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 37 2 UNICORN DAQ concepts 2 3 Quick Start Guide 38 Step Action 4 Click the End icon in the System Control module to stop acquiring data from the cell cultivation Result A dialog opens asking you to confirm that you want to save the partial result 5 Click the OK button to save the result 6 In the Evaluation module e Locate and select the corresponding result file in the Recent Runs list e Click the Report icon e Choose a report format and click OK
49. E Bioreactor 28 9846 82 AA 201 A Audit trails The System tab The illustration below shows a System tab of the Audit Trail dialog box BA Audit Trail File Edit View Help Global BF Wave 03 29 2010 10 06 29 System log started on computer SEHC88YDP2JL Run End Manual 03 29 2010 10 06 29 Session start on Wave z 03 29 2010 10 06 51 Eror Controller card 1 error 2012 No response Profibus 2 on System Wave Alarms Warnings 03 29 2010 10 06 51 Error Controller card 1 error 2012 No response Profibus 4 on System Wave Enos 03 29 2010 16 43 26 User default at computer SEHC88YDP2JL in control of the system 03 29 2010 17 00 35 User default at computer SEHC88YDPZJL left control of the system Calibration 03 30 2010 09 07 23 System log started on computer SEHCBBYDP2JL g 03 30 2010 08 07 23 Session start on Wave Jorermaten Timezone ein 014 52 06 User default at computer SEHC88YDP2JL in control of the system The table below describes the items which can be displayed on a System tab Item Displays Run End Manual e the times for start and completion of a run e the times for manual operation Alarms Warnings alarms and warnings defined in the strategy for the sys tem Errors system errors Calibration monitor calibration operations Information system log started stopped Timezone the time zone where the activity was logge
50. GE Healthcare UNICORN DAQ 1 0 User Manual for WAVE Bioreactor O UNICORN Table of Contents Table of Contents 1 Introducing UNICORN DAQ Be seninee eel pirimten annem rt arr pettenne nice tire nrer sites 7 ernre 1 1 About UNICORN DAQ oe 127 Abo tthis MANUO accsscsscscesssccaatssacecceccseccesaceccsatenssssenciecsecssasectiisnnssspeossedecassddodbtlcessesanesnnsanzeseedsdecccesofetbs UNICORN DAQ concepts s ssssssessssessessssessssesessessessssesessesessessesessssessesessesseseseese 2L COMICS HE CSTIMMIO NS usain N a aE 2 2 The UNICORN DAQ user interface 2 2 1 UNICORN Manager u s 2 2 2 The System Control module a 223 FRE Evaluation module sicccsscsssccisieasiictasicestsscceascxosgsascesasacsaysscccorinassossiuadn EAEN 224 Search TUNGUHIN S mneniasinarnsunenssnsn eniai A E Rn 2 2 5 Help functions 2 2 6 Snapshots a3 2 3 QUICK Start Guide wee eeccceeccsssseescsssesssssstesssssessssssesesssuesessssssssssseessssueessssueessssuessssuuesssssesssssnesssesseseseee Astaat Nien E NE 3 1 How to install the Converter 100 oe 3 2 Software installation and system definition 3 2 1 Preparations before installing UNICORN DAQ 3 2 2 How to install UNICORN DAQ wauessesssssssssssssssesssssssesssssssssssseescsssssssssssesesssssessssuesssssetessssutessssuesesssess 3 2 3 Windows 7 post installation settings eesessssssssesssssssscsssssssssssssssssssesssssssssssssssssss
51. How to search for text in audit trail files 204 The table below describes how to search for text in audit trail files Step Action i Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed If you want search the currently open audit trail file e press the CTRL F keys or choose Edit Find in current log Note The currently open audit trail is the one displayed in the Audit trail dialog box and depends on the selected tab Global or a System e type the text string you want to find in the Find Text dialog box and click the Find button e press the F3 key or choose Edit Find next to find the next occurrence of the search string If you want to search all the existing audit trail files either Global or System e choose Edit Find in log files e type the search string choose whether to search Global or System audit trail files in the Files at drop down box and click the Find button Find Text Text Files at Global Wave Result The search results are displayed on a new temporary tab named Find result UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA A Audit trails How to print audit trails The table below describes how to print an audit trail file Step Action 1 Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed 2 e Select the Global tab or a System ta
52. Manual for WAVE Bioreactor 28 9846 82 AA 85 5 Files and folders in UNICORN DAQ 5 2 How to open and preview files Step Action 2 e Locate and double click the result file JX Recent Runs Files Find Example Result j ExampleResult GF u Example Result001 wu u Example Result003 Example Resultoo4 i ExampleResult GFOO1 i ExampleResult GFO02 Result The result file opens Note The File Navigator opens by default in the Evaluation module If it has been closed select View File Navigator in the Evaluation module The File Navigator is described further in Section 7 2 How to use the File Navigator on page 124 Quick View Quick View is a preview function for result files to make it easier to select the correct result file You can preview the first curve in the first chromatogram You can also select to view another curve as default by selecting another curve number in your User Attributes settings see Section 4 3 How to change your passwords and user attributes on page 75 Several files can be opened for comparison How to use Quick View The table below describes how to preview result files in Quick View Step Action 1 Select one or more result files in the Result window of the UNICORN Manag er 86 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 5 Files and folders in UNICORN DAQ 5 2 How to open and preview files Step Action 2 e Choose File Quick Vie
53. NICORN software is used both in laboratories for analytical purposes and in the biochemical process industry UNICORN is a trademark of GE Healthcare Operating environment UNICORN DAQ runs on a PC under Microsoft Windows XP 32 bit or Windows 7 32 bit It is designed to run under English keyboard settings Windows functions Most Windows functions are also available in UNICORN DAQ including e cutand paste e right click short cut menus Note Drag and drop is not available File and folder handling in UNICORN DAQ also differ from the general Windows file manager standard 8 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Compatible systems 1 Introducing UNICORN DAQ 1 1 About UNICORN DAQ UNICORN DAQ can be used with a number of WAVE Bioreactor cell culture systems ranging from the WAVE Bioreactor System 20 50 to the WAVE Bioreactor 500 1000 Software modules The UNICORN DAQ software consists of three integrated modules Module Function UNICORN Manager File handling and administration e g definition of systems and user profile etc System Control To monitor and store data from the cell culture processes online using a template based data acquisition method Evaluation To evaluate and present stored data from the cell culture processes Note All modules are active when the program is operating and are not closed when they are minimized A minimized System C
54. ORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 3 The Evaluation module Toolbar icons in the Evaluation module The table below describes the toolbar icons in the module Icon Function The Open icon displays all available result files and result folders in the Open Result dialog box 7 The Open Curves to Compare icon opens the Open Curves to Compare dialog box which is used to select and open curves for comparison The Save icon saves the edited result file The Print icon opens the Print Chromatograms dialog box AG The Report icon opens the Generate Report dialog box which is used to select a report format The View Documentation icon opens the Documentation dialog box which is used to view and edit the result documentation Ly The Chromatogram Layout icon opens the Chromatogram Layout dialog box which is used to select and format curves and display items in the chromatogram E 26 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 4 Search functions 2 2 4 Search functions Introduction This section describes the general search functions that can be used to locate for example chromatograms curves and text strings in UNICORN DAQ These functions can be used in several program modules and dialog boxes Search the Folder list The search will take place in the displayed folder onl
55. Restart required Windows 7 e Microsoft Visual C 2008 Redistributable Package x86 e OPC Core Components Redistributable x86 101 2 e SetSystemPermissions Restart required Note Aversion of Adobe Acrobat Reader is also distributed on the instal lation CD Step 3 License agreement and user information This table describes how to complete step 3 of the UNICORN DAQ installation Step Action 1 e The Welcome dialog box is displayed e Click the Next button to continue 2 e The UNICORN DAQ Software License Agreement dialog box is displayed You must accept the license agreement to install UNICORN DAQ e Click the Next button to continue 3 e The User Information dialog box is displayed Type your name company and the product serial number of the software The serial number can be found on a sticker on the back of the installation CD cover e Click the Next button to continue UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 51 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ Step 4 Select Destination Folder 52 In the Destination Folder dialog box you choose the installation folder for the UNICORN DAQ software By default this will be C UNICORN e If this is suitable click the Next button to proceed or e Follow the instruction below to choose another folder ie UNICORN DAO 1 0 Workstation Ins
56. Snapshots 32 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 15 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 1 UNICORN Manager 2 2 1 UNICORN Manager Introduction The UNICORN Manager is mainly used for file and folder administration The UNICORN Manager windows 16 The module contains two windows the Methods window and the Results window UNICORN DAQ 1 0 for WAVE Bioreactor provides template methods for the data acquisi tion but not the facility for the user to create or edit own methods Consequently the Methods window will not be used and can be minimized to save space for the Results window The UNICORN Manager is shown below e 4 B mozio 2010022 1004 MOONI IAA IOGT 1647 2100016 1698 Z1OGDE OPS so100719 IF OGM 172 20100709 1247 2010970 12097 2010 07 05 10 45 2010 6700 16 95 Wom BIOETH 10 WoA ZOO 16 08 moasa DODGER 1554 oads HH POOH 187 WIGHT EAE 2010024 1842 WOO 2010024 18 99 W0 aae 20109 08 0948 210OH O8 18 02 201002 1300 POOL ISS 201006 2 18 57 MOOS A E42 NOS 1442 MOORE 20100 1495 201009221098 2010 4922 10 48 201003103708 20108191704 201009100030 2010415 00 90 2010 09110 2010 00 11 09 00 2010 09 11 09 08 2010 00 11 O06 201007110055 2010 69 41 ORE 2010 07 20 1827 2010 07 20 48 27 2010 07 08 14 2010 07 06 14 56 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA The Results window 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ use
57. System Control module windows may open if UNICORN DAQ was set up to control more than one system at the installation Unlike other versions of UNICORN DAQ a Method Editor module is not activated for UNICORN DAQ 1 0 for WAVE Bioreactor A template method for data acquisition is supplied with the WAVE Bioreactor strategy and should be selected when a cell cultivation run is started to collect data from the run in the System Control module Note If the access rights are limited to only some modules the other modules will not open UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 69 4 General system operations 4 1 Log on routines and log off routines Log off after you are finished Always log off when you leave the computer to prevent others from accidentally changing or deleting your files or disturbing your UNICORN DAQ runs There are two ways to log off in the UNICORN Manager e Select Tools Logoff or e Click the Logon Logoff icon gt i Note In case your access to the UNICORN Manager is restricted you will still be able to log off Unlocked Log off It is not recommended that you log off and leave a running UNICORN DAQ system un locked This means that another user may accidentially interrupt the data collection of the cell cultivation in progress Automated workstation lock or logout 70 The system administrator may set an automatic workstation lock or log off after a specified time for a
58. able to type new comments on the Evaluation Notes sub tab Note Click the Find button to search for a specific text string in the Notes Logbook The Logbook tab displays what happened during a method run You can view information concerning alarms the method manual changes during the run errors etc Note Click the Find button to search for a specific text string in the Logbook Evaluation Log The Evaluation Log lists all of the evaluation operations that you have performed for the result file during all ses sions including at the end of the method Method Information The Method Information tab displays information about the method such as the method name the target system and the date of the last change Information about the strategy includes name date and size There is also a sub tab for Signatures Result Information See The Result Information tab in this section The Result Information tab 170 The Result Information tab displays information about the result file such as e the result file name e the system that was used e the last date it was changed Information about the strategy includes name date and size The Run Summary sub tab is a summary of the run expressed in volume or time per block There is also a sub tab for Signatures and a sub tab where all Snapshots that have been taken during the run are displayed UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 A
59. adds a blank page to the report Delete Page This button deletes the current page from the report Exit This button closes the Customize Report window How to add and delete report pages The table below describes how to add or delete report pages in the Report Editor If you want then to add new pages e click the Add Page toolbar button Result A new page is added after the last page to delete a page while e select the page with Next Page or Prev Page in One Page mode e click the Delete Page toolbar button and confirm the deletion to delete a page in Two e select the page with Next Page or Prev Page Page mode e click an object on the page e click the Delete Page toolbar button and confirm the deletion How to change the page layout The page layout is changed in the Page Setup dialog box The table below describes how to set up the page layout Step Action 1 Double click anywhere on the report page in the Report Editor not on an object Result The Page Setup dialog box opens UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 153 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report 154 Step Action 2 Type new values for the Margins if necessary Select the appropriate Settings and Unit Click the First Header tab Note An extra Header tab will appear if you de select th
60. anual instructions during a data acquisition 116 Overview 101 Toolbar buttons 114 Warnings 119 System Control status bar Description 115 System Control Toolbar Manual command buttons functions 114 Windows buttons 115 212 System data How to backup 79 How to restore backup da ta 81 System operation Automated workstation lock or logoff 70 How to connect to a sys tem 78 Unlock system locked by other users 71 7 Toolbar icons In the System Control mod ule 23 U UNICORN Manager Limited access to 18 User Account Control How to disable on Windows 7 46 W Warnings Description 119 Windows 7 User Account Con trol How to disable 46 Y Y axis How to choose the Y axis scale 133 Z Zoom function How to enlarge parts of a curve 146 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA For local office contact information visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com unicorn imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Drop Design Cellbag UNICORN DAQ WAVE WAVE Bioreactor and WAVEPOD are trademarks of GE Healthcare companies All third party trademarks are the property of their respective owners 2010 General Electric Company All rights reserved First published Nov 2010 All goods and services are sold s
61. ards to sleep while a run is in progress This section describes how to disable power save settings that are in conflict with UNI CORN DAQ operations Disable the computer power save setting The table below describes how to disable the Windows 7 power save settings Step Action 1 e Click the Windows Start button and e Choose the Control Panel 2 Click the Systems and Security heading 3 Click the Power Options heading 62 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 3 Windows 7 post installation settings Step Action 4 Click Change plan settings for the selected power plan a ee GOB gt conoi Panel Sytem and Security Power Options 6z Seah Conert Panel Select a power plan Power plans can help you maximize your computer s performance or conserve energy Make a plan active by selecting it or choose a plan and customize it by changing its power settings Teil me more about power plans Preferred plans Balanced recommended Change plan settings Automatically balances performance with energy consumption on capable hardware Power saver Change pian settings Saves energy by reducing your computer s performance where possible Show additional plans 5 e Choose the option Never for the setting Put the computer to sleep and e click the Save changes button Change settings for the plan Balanced Choo
62. atch 50L Chromatogram name Number given automatically duringarun 11 or a name defined by the New_Chro matogram instruction Curve name Curve type Rate How to choose curve name appearance You can choose to view only part of the curve name The table below describes how to do this Step Action 1 Open a result file 2 Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed 3 Click the Curve Names tab UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 137 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 2 The Curve tab and Curve Names tab Step Action 4 e Select the appropriate boxes for Curve name appearance e Select the appropriate Curve legend position e Click OK Note It is usually sufficient to select the Curve Name option if only one chromatogram is being evaluated However confusion can arise when more than one chromatogram is shown so more complete names might be necessary 138 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 3 The Curve Style and Color tab 7 4 3 The Curve Style and Color tab Introduction All curves within a chromatogram are represented by a default color and line style Curves imported into the chromatogram or newly created curves are automatically as signed a color and li
63. available See Section 5 3 How to arrange and lo cate your files on page 88 for more information UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 2 How to use the File Navigator How to open a Recent Run The Recent Runs list shows all the available recorded recent runs based on the selected user preferences The table below describes how to use the Recent Runs list to locate and open a result file Step Action 1 e Click the Recent Runs tab Result The Recent Runs list opens in the File Navigator Recent Runs Files Find Recent Results LU erification fed batch 20L ba Note Until the files and chromatograms in the list have been opened and saved they are noted in bold text When they are opened and saved the text is changed to plain text e f needed click the Refresh button in the bottom of the File Navigator Result The Recent Runs list is updated with all runs that were performed since the File Navigator was opened the last time e Locate the desired run e Double click the file Result The result file opens in the Evaluation module Note Note Click the signs to view or select individual chromatograms from the result files Individual result files can be selected and removed from the list by clicking the Remove button The Remove all button clears the whole list Remove only clears the list the files are not del
64. b e Ifyou want to print other audit trail files than the currently open then open the file s as described in How to view older audit trail files above 3 Choose File Print to print the file s displayed in the dialog box How to renew audit trail files The audit trail file is renewed at regular intervals between 1 to 30 days The old audit trail file is then saved automatically together with all the previous audit trail files The table below describes how to set the renewal interval and how to renew the audit trail immediately Step Action 1 Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed 2 Choose View Renew interval Result The Renew interval dialog box is displayed Renew interval Next renew The log file will be renewed at Monday April 05 2010 a Interval Renew log file every 7 days Close UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 205 A Audit trails Step Action Set renewal interval e Type the desired interval in the Interval field The standard value is 7 days e Click the Set button Result The new Interval setting will take effect after the next scheduled re newal or immediately if the Renew now button is clicked Renew now e Click the Renew now button to start a new audit trail file The old file is saved automatically Example If the audit trail is set to renew every 7 days and you clic
65. be updated during method run Note The Auto update function cannot be used for external value entries 116 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 3 Manual instructions 6 3 2 Manual instructions How to use manual instructions Manual instructions are entered in the Manual instructions dialog box in the System Control Step Action 1 Select an instruction group and a component in the Instructions field 2 Select instruction parameters in the Parameters field 3 Click the Insert or Execute buttons as needed See the descriptions of the different functions below How to enter a Set_Mark instruction In UNICORN DAQ a Set_Mark is used to enter essential information into the data acqui sition result This information will be visible in the chromatogram but also more impor tantly in the Logbook It is recommended to enter a Set Mark describing the settings and selections for e WAVE Bioreactor set points e WAVE Bioreactor calibrations The table below describes how to add a manual Set_Mark instruction Step Action 1 e Select the Other instruction group and e select the Set_Mark instruction 2 Type the Set Mark text in the Mark text field 3 e Click the Execute button to add the mark text to the chromatogram and the run log or e Click the Insert button to add the Set Mark to a list of instructions The instructions will b
66. bes some of the general UNICORN DAQ settings In this chapter This chapter contains this section Section See page 9 1 Curves 187 186 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 9 System settings 9 1 Curves 9 1 Curves Introduction This section is a short description of the Curves system settings The Instructions dialog box The illustration below shows the Instructions dialog box with the Curves instructions selected Store ON Mee between samples 10 000 s Store ON Time between samples 10 000 s L Airflow Store ON Time between samples 10 000 s F_Aitflow Curve settings The curve settings determine which monitor signals that will be stored as curves in the result file Verify that Store ON is set in the Instructions dialog box for all signals that are to be stored Note Ifa curve is set to Store OFF data from the specific monitor cannot be displayed in the curves window during a process run The data will not be recorded in any way UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 187 9 System settings 9 1 Curves Store and Time between samples The table below describes the function of the two curve settings Setting Function Store OFF ON This setting determines whether the curve data is stored or not Time between sam This setting determines with which frequency curve data ples is recorded It does not affect the reading frequency o
67. cepts are organized in alphabetical order Chromatogram A chromatogram is a collection of data represented by a number of curves that have been created during a cell cultivation including temperature pH etc Original raw data curves cannot be deleted or modified They can be used as a basis for evaluation procedures and subsequent creation of new curves A chromatogram can also contain curves that have been created and saved during an evaluation session Curves The monitor signals from the cell cultivation run as well as data entered using the Exter nal value instruction are displayed graphically as curves Note Log text entries and similar events from the run are treated as curve data in UNICORN DAQ even though they are not shown graphically as curves in the chromatogram Method The data acquisition instructions for a cell cultivation run are defined in a Method A ready made template method is supplied for UNICORN DAQ Result files UNICORN DAQ creates Result files from the data acquisition The Result files contain e Run data from the monitors in the WAVE Bioreactor e Documentation from the run Example Logbook entries manual instructions etc UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 13 2 UNICORN DAQ concepts 2 1 Concept definitions e Saved results from evaluations of the run data Strategy Part of the UNICORN software is specific for the system that it is set up
68. create a new user on page 72 How to start the program Note If UNICORN DAQ is already started by a previous user proceed to How to log on There are two ways to start the program If you start with Then aUNICORNicononyour double click the icon desktop Al ay UNICORN DAQ 1 0 the Windows Start locate the program under All programs Unicorn and click menu in Windows XP the UNICORN logo the Windows Start locate the program under All programs Unicorn and click menu in Windows 7 the UNICORN logo 68 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 1 Log on routines and log off routines How to log on The table below describes how to log on to UNICORN DAQ Step Action 1 e Select Tools Logon in the UNICORN Manager module or e Click the Logon Logoff icon in the UNICORN Manager module gt i Result the Logon dialog box is displayed Note You do not have to perform this step if you start up UNI CORN DAQ When you start UNICORN DAQ the Logon dia log box is automatically displayed 2 Select your username from the list 3 Type your password optional 4 Click OK The program modules The program has three modules When you start the program and log on you work in the UNICORN Manager module UNICORN DAQ also automatically opens the System Control and the Evaluation modules These modules are minimized until you activate them Up to four
69. cribes how to add objects to the report The various objects are de scribed below this table Step Action 1 Click the appropriate icon in the Report items toolbar or Choose an object from the Insert menu T Freetext Picture Text Method Chromatogram Documentation Evaluation log Press and hold the left mouse button on the report page and drag out a box to the size of the item you want to insert Note The mouse pointer shows a symbol for the type of item you have selected Release the mouse button Result A Setup dialog box opens The dialog is specific to the type of item that you want to insert Select the desired options and click OK Result The object is inserted onto the page Note If you want to edit an object later double click the object box UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 155 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report How to add free text The table below describes how to add free text to the report Step Action 1 e Click the Free Text icon it e Press and hold the left mouse button on the report page and drag out a box to the size of the text Release the button Result The Setup Free Text dialog box opens 2 e Type text in the edit field e Select if the text is to start on a new page e Select if the text box should be automatically sized e Select
70. cuting a manual instruction as opposed to starting with the supplied template method the results are automati cally stored in a folder called Manual Runs The Manual Runs folder stores the ten most recent results from your manual runs To save a result file from the Manual Runs folder more permanently you need to move or copy it to another location UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 3 Manual instructions 6 3 3 Warnings 6 3 3 Warnings Introduction Warnings are displayed regardless of the activity currently in progress in UNICORN DAQ You will be notified that for example an exceeded limit in a running WAVE Bioreactor system has triggered an alarm even if you are evaluating data or monitoring a cell cul tivation run on a different system Warnings are also recorded in the logbook for the run Note The WAVE Bioreactor will continue the run It will not be set in a pause state Limits for monitor signals The WAVE Bioreactor settings in the base unit or WAVEPOD determine the acceptable limits of monitor signals during a cell cultivation run If these limits are exceeded an alarm will be triggered by the unit and a corresponding warning dialog box will be dis played on the screen by UNICORN DAQ In a network system In a network installation warnings are displayed on the controlling station and all stations viewing the system
71. d 202 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA How to view previous audit trail files A Audit trails Anew audit trail file is created at regular intervals and the old audit trail file is automat ically saved See How to renew audit trail files below for more information The table below describes how to view previous audit trail files Step Action 1 Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box displays the current audit trail 2 e Choose File Open to display the Open audit trail dialog box Open audit trail Type File Global M Name 2009110200 2009122200 2010011300 2010020300 2010022200 20100303 Browse Date Monday November 02 2009 Tuesday December 22 2009 Wednesday January 13 2010 Tuesday February 09 2010 Monday February 22 2010 Wednesday March 03 2010 Friday March 12 2010 3 e Choose Global or a System in the Type drop down box e Select the file s to open from the File list Use the CTRL key if you want to open several audit trail files at the same time Click the Browse button if you want to open a file which is not listed Note Files are named by date and serial number e Click OK Result The audit trail file s is now displayed on a new temporary tab UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 203 A Audit trails
72. d dial up or VPN network connection Choose homegroup and sharing options Access files and printers located on other network computers or change sharing settings See also SS Troubleshoot problems HomeGroup Diagnose and repair network problems or get troubleshooting information Internet Options Windows Firewall 64 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 3 Windows 7 post installation settings Step Action 3 Click the Change adapter settings link Result The Network Connections dialog opens 4 Double click the network interface card you need to change Result The Network Connections dialog opens A e 117 676 932 906 356 317 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 65 3 Installation 3 2 Software installation and system definition 3 2 3 Windows 7 post installation settings Step Action 5 Click the Properties button Result The Properties dialog opens aa Peters AW Broadcom NetXtreme 57x Gigabit Controller a E Aiow the computer to tum o Aligw this device to wake the computer Only allow a magic packet to wake the computer Waming F this is a laptop computer and you run it using battery power allowing the network adapter to wake the computer could drain the battery more quickly t might also cause the laptop to become very hot
73. d Y axis values for the new position are displayed together with e the new position in relation to the reference point e the minimum maximum and average values for the curve interval be tween the reference point and the new position 134 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 Howto optimize the presentation of a chromatogram Introduction This section describes some of the ways you can optimize the presentation of a chro matogram In this section This section contains these sub sections Section See page 7 4 1 How to make changes in the Chromatogram Layout dialog box 136 7 4 2 The Curve tab and Curve Names tab 137 7 4 3 The Curve Style and Color tab 139 7 4 4 How to change and fix the axes 142 7 4 5 How to save and apply a layout 144 7 4 6 How to show part of a curve 146 7 4 7 How to change the size of the Logbook marks 147 135 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 1 How to make changes in the Chromatogram Layout dialog box 7 4 1 How to make changes in the Chromatogram Layout dialog box Instruction The Chromatogram Layout dialog box is used to make changes regarding chromatogram presentation The main features of the Chromatogram Layout dialog box regarding chromatograms are described in the
74. e The Flow Scheme is a graphical representation of the WAVE Bioreactor system During a run the Flow Scheme displays the system configuration and the status of the system components Monitor signals are displayed numerically See the illustration below poz on aa 37 8 sp 37 0 ea gt 7 24 SP 7 25 Time to next feed 8 min Weight kg Cumulative 25 47 Harvest L 5 SP 10 00 Feed 24 L UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 21 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 2 The System Control module The Logbook pane The Logbook pane displays all recorded events during a cell cultivation run e g data acquisition method start and end and manual instructions such as Set mark or External value See the illustration below 368 09 h Left Bag 02 Deviation 366 09 h lt ACK gt default Left Bag O02 Deviation 368 10 h Left Bag O2 Deviation 368 11 h Set_Mark New DO set point chosen Manual 368 11 h lt ACK gt default Left Bag 02 Deviation 366 14 h Set_Mark Decreased DO control cycle time Manual 368 19 h Set_Mark Increased feeding pump speed to 50 rom Manual 368 55 h Set_Mark feeding terminated Manual The Status bar 22 The Status bar in the bottom of the System Control module displays the current status of the cell cultivation run See the illustration below For Help press F1 Run Main No watch Controlled By defau
75. e option to have the same header on all pages The First Header tab is used for the first page header only and the Header tab is used for all subsequent pages Select all the items you want to include in the header from the Select Items list Click the Font button to change the font for all items if necessary Type header text in the Free text box and click the Font button to alter the default font if necessary Type the report title in the Report title box and click the Font button to alter the default font if necessary Select the Logo check box and click the Browse button if you want to locate and select a logo image file Select the Alignment for the logo if necessary Note The logo file must be in bitmap format bmp and smaller than 64 kB Larger logo files or files in other formats must be inserted as Picture objects If you want to have a line under or over the header select the appropriate option in the Layout field Repeat steps 3 to 6 on the Footer tab and the subsequent pages Header tab Note All Header and Footer tabs contain the same options You can have all information in either the header or footer or split information between the header and footer as re quired Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report How to add objects to the report The table below des
76. e Create New User dialog box 9 Click OK Result The new user is created and added to the User Setup list 10 e Click Close or e Click the New button and repeat steps 1 9 to create more users Home folders 74 Each user must be assigned to a home folder The Default folder can be used if you do not want to assign an individual home folder Note If you create a home folder on the C local drive it will not be accessible from other computers If you select a network make sure that is ad dressed by the same drive letter from all computers in the network UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 3 How to change your passwords and user attributes 4 3 Howto change your passwords and user attributes Introduction Every user can change his or her passwords and some user attributes even if user ad ministration is handled exclusively by the system administrator The changes are made in the UNICORN Manager How to change passwords The table below describes how to change your logon and signature passwords Step Action 1 Select Administration Change Password Result The Change Password dialog box opens Type your old logon password in the Old text box Note Your passwords will only be shown as asterisks Type a new password in the New text box Repeat the new password exactly in the Confirm text box Repeat steps 2 to 4 in the Signature password
77. e Logbook text alignment option Horizontal Vertical Fly over displays the text if you place the mouse pointer over the generated mark 4 To filter the type of Logbook information overlaid on the Curves pane do the following e Click the Filter button Result The Filter Logbook dialog box is displayed e Select the appropriate check boxes and set the maximum block depth e Click OK to return to the Curve style and Color tab 5 Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 109 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 4 The Flow Scheme pane 6 2 4 The Flow Scheme pane Introduction The flow scheme is a graphical representation of the WAVE Bioreactor system that shows the current status of the cell cultivation run During a run the flow scheme displays the system configuration the status of the modules and the monitored signals with numer ical displays Illustration The illustration below shows an example of a flow scheme for a run pox on Temp C on Angle 37 8 sp 32 0 027 on pent 5 SP auto Air Vinin on Speed rpm oa 19 9 sp 20 0 Time to next feed 8 min pH oon 7 24 sp 2 25 Weight kg Cumulative 25 47 Harvest B L z SP 10 00 Feed 24 L How to stretch a flow scheme The flow scheme can be stretched to fit the screen To do this
78. e Preview button to open the report in Notepad e Click the Print button to print the report without any preview Click the Finish button to generate and save the report the report Step 2 3 198 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA A Audit trails Appendix A Audit trails Purpose The audit trail provides the system administrator with a full record of UNICORN DAQ usage and system activity The different types of audit trail files There are two types of audit trail files global files and system specific files The global audit trail files e are saved on the server disk in a network installation e are examined via a network connection The system audit trail files e are saved on the local station to which the WAVE Bioreactor system is physically connected e canbe examined from the local station without logging on to the network e canbe examined from any computer in a network installation Tabs of the Audit trail dialog box The Audit trail dialog box has two or more tabs one Global tab and a System tab for each installed system e The global tab displays usage information for the complete UNICORN DAQ installation e The system tabls displays usage information for a chosen system UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 199 A Audit trails How to view the audit trail files The table below describes how to examine the global and system audit trai
79. e performed at first when the Execute button is clicked UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 117 6 How to monitor and acquire data from cell cultivation runs 6 3 Manual instructions 6 3 2 Manual instructions Note You should normally only execute the Set Mark instruction when a data acquisition is already in progress If you enter a Set Mark before a data acquisition has been started using the template method you will start a manual run Refer to How to save manual results below for information on how to save results from manual runs to ensure that any data that you acquire manually is not lost The buttons of the manual instructions dialog box The table below describes the functions of the manual instructions buttons Button Function Insert This button places the current instruction in the list at the bottom left of the dialog box Delete This button deletes the selected instruction from the cur rent list only One instruction can be deleted at a time Execute This button e executes all instructions in the list at the same time or e executes the currently marked instruction if the list is empty Close If you click the Close button without first clicking the Exe cute button commands in the list e will not be executed e will be deleted from the command list How to save manual results 118 When you choose to run the data acquisition manually by exe
80. e such as cell type culture media batch numbers and calibration regulator parameters The notes can also be used during run and evaluation Run notes and Evaluation notes but only Evaluation notes can be edited after the run has ended The notes can be printed as part of a report at evaluation cov _ Heo 96 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 1 How to start the cell cultivation data acquisition Step Action 2 e Select a system if several WAVE Bioreactor systems are available e Make sure that Template is selected e Choose the template method in the Template list 3 Click the Run button Result The Start Protocol opens with the Notes page and the Start Notes tab selected UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 97 6 How to monitor and acquire data from cell cultivation runs 6 1 How to start the cell cultivation data acquisition Step Action 4 e Enter information about the data acquisition and e click the Next button to proceed Result The Result Name page opens Result Name e Choose a directory where the result will be saved e enter a Name for the result and e click the START button Result UNICORN DAQ starts monitoring the data from the current cell culti vation run Tip In the Result field of the Result Name page you can select the
81. ecommend thot the default user is deleted when regular user profiles are created 72 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 2 How to create a new user How to open User Setup All user administration is performed in the User Setup dialog box in the UNICORN Man ager module It is accessible only to authorized users and the default user User Setup is found on the Administration menu e Choose Administration User Setup The User Setup dialog box The illustration below shows the User Setup dialog box User Setup OPC user OPC user UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 73 4 General system operations 4 2 How to create a new user How to create a new user The table below describes how to create a new user Step Action 1 Click the New button in the User Setup dialog box Result The Create New User dialog box opens 2 Enter a user name in the User name text box 3 Enter the full name of the user in the Full name text box 4 Enter the job position of the user in the Position text box 5 Select or create a Home folder e Selecta Drive and a folder from the Name drop list and proceed to step g or e Ifyou need to create a new home folder proceed with step 6 6 Click New Result the Create New Folder dialog box opens 7 Select a Drive and type a folder name 8 Click OK to create the folder and return to th
82. efault report format check box if desired The format is saved as DEFAULT Click OK Result The new report format is saved and available in the Format list 168 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 7 Run documentation 7 7 Run documentation Introduction The full documentation for a run is stored in the result file This section describes e some of the contents of the run documentation and e how to view and print the run documentation How to view and print the run documentation The table below describes how to view and print the run documentation Step Action 1 Open a result file 2 e Choose View Documentation in the Evaluation module or e Click the view Documentation icon 0 Result The Documentation dialog box opens See further information about some of the tabs below 3 Click the Print button Result The Print dialog box opens 4 Select the documentation items you want to print and click OK Result The documentation is printed on the default Windows printer UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 169 7 How to view results 7 7 Run documentation The tabs of the Documentation dialog box The table below describes the contents of some of the Run Documentation tabs Documentation tab Contents Notes The Notes tab displays the notes that you have made at various times during the method run You are also
83. ersion for the software to work correctly Please check that all computers connected to the UNICORN server you are connecting to is of the correct version If another version is detected on one of the other computers connected to the UNICORN server those computers have to be upgraded to the same version you are currently installing e You can exit the installation at any point by clicking on either the Cancel button or the Exit button If you do this however the installation will be incomplete and the software cannot be used e After the installation the installed files are compared with the original files on the installation CD to make sure that no files have been corrupted during installation The criteria used for the file comparison are name size version and checksum UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 49 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ Do not copy the CD ROM or decompress the files UNICORN DAQ is supplied on a CD ROM Files on the CD ROM are compressed and cannot simply be copied onto the hard disk During the installation procedure the required folder structure is created on the hard disk and the files are decompressed Do not at tempt to decompress the files using any other file decompression utility Step 1 Insert the Setup CD Follow the instructions in the table below to begin the installation Step Action 1 For n
84. es and folders in UNICORN DAQ 5 4 How to copy delete rename and backup files and folders Step Action 2 e Select File Delete or e Right click and select Delete from the shortcut menu or e Press the Delete key 3 Confirm the delete action in the confirmation dialog box Backup security Backup copies should be taken regularly to avoid data loss in the event of hard disk failure or accidental deletion You can use the function Copy to External to save your files on the network server Note GE Healthcare cannot accept responsibility for the replacement of results that were lost as a result of computer failure or other incidents 94 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 Howto monitor and acquire data from cell cultivation runs Introduction This chapter describes how to monitor and perform data acquisition from cell cultivation runs from the System Control module It also describes how to perform manual com mands Note that the UNICORN DAQ software cannot be used to control but only to monitor the operation of the WAVE Bioreactor system and to collect result data In this chapter The chapter contains these sections Section See page 6 1 How to start the cell cultivation data acquisition 96 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 100 6 3 Manual instructions 113 6 4 If the network connection fails
85. es that have been installed previously e After installing the strategies click the Next button to proceed Result The System Setup Control Unit X System dialog opens 3 e Inthe Select Control Unit for System field select the control unit you wish to use for the WAVE Bioreactor system and e Click the Change button Result The System Setup Control Unit X System dialog opens System Setup Control Unit 1 System Set the parameters for the Control Unit 1 System First set the system name and the strategy that shall be used for the system Then set the IP address for the system System name System type Strategy Controller IP address System 1 Wave Wave_20 50_ 127 CU 960 Advanced 192 168 66 n Select the users that shall be able to access the Control Unit 1 System V default UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Cancel 59 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ Step Action 4 e Enter a system name in the System name field e System type the strategy you selected in the wizard page and the con troller type will all be shown in the corresponding fields If this is not correct choose the appropriate entries from the menus e Enter the IP address for the controller unit This address is printed on the back of the unit e Select the users that are to have access to this sy
86. escribes how to attach a file Step Action 1 The Attachments dialog box is displayed Attachments System information Computer amp operating system information AKTA hardware informatior Integrity check lt Back et Cancel Help 192 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Step 10 Error reporting 10 1 How to generate problem reports 10 1 1 How to generate a problem report from the UNICORN Manager Action e Depending on the character of the file to be attached select the appro priate tab Result System log or Global log e Attach the file Click the Add button Selecta file in the dialog box and click the Attach or OK button Result The selected file is added to the tab in the Attachments dialog box Note To remove a file select the check box and click the Delete button To include more information in the report select the appropriate check boxes in the System information field By default all options are checked Computer amp operating system information Asummary of the computer and operating system information for example type of processor processor speed RAM hard disk capacity and printer Integrity check When UNICORN is installed a checksum calculation is performed on the stationary files dll and exe for the system An integrity check means that a new checksum calculation is performed for the same files in their folders This new calculated
87. esesssssssssssssssssssssssssscssssssssssssssssssssesssssssssssssssssssssesssssssssssssssscees 131 7 3 2 TRE chromatogram Window oe eeesesessssssssssssssssssssssssssssssssssssssssssssssssesceceeeneeeeenseceeneeceeccencencenceceeeeceee 132 7 4 How to optimize the presentation of a chromatogram a 135 7 4 1 Howto make changes in the Chromatogram Layout dialog DOK ui ssssssssssssssssesscceeseee 136 7 4 2 The Curve tab and Curve Names tab oiseeeeseesesssssssssssssssssssssssssssssssssssssssesssesceesceneeececcececencceeceeeee 137 7 4 3 The Curve Style and Color tab 0 139 7 4 4 Howto change and fix the axes 142 7 4 55 Howto save and apply a layout 144 7 4 6 How to SHOW part OF Q CUEVE ceeeeeeesessssssesesssssessesssssssssssssssssssssssssssessesennseeenenceceenseneecececeeneeeeeeeseeeee 146 7 4 7 Howto change the size of the Logbook Marks iesasssssssssssssssssssssssssssssssssssssesecccceeesececcccceceees 147 7 5 How to print active chromatograms ues 148 7 6 How to create dnd print reports ou 150 7 6 1 Howto create and print a customized report a ASL 7 6 2 How to edit an existing report format ouue seessesessssesssssssssssssssssssssssssssssssssssscessseeeeceeceenecccecceecceee 167 TI RUM CO CUM SMEIEIOMN a cicecccsssccececeassstessectcnessscseeesrechstesinaccardbsccsteetessusts aacsssustaasnietvieustsivasesneanetiene 169 How toredit results cha dec conucctacepsseraverbtctesseiucichcetadntestertecdsvenscenpisalavdesseusteatasdeioes 172 8 1 How to e
88. esssesssssssseeseesees General system OperdtionS sennccinen seen eene as 4 1 Log on routines and log Off routines oi ee eeccccssssssssessessssssccsssssssssssssseseesesssssssussuessesseceesesssssssseesses 4 2 HOW TO ChEGLE A MEW USEF ssysssssscccccscsedstcnsesecansnapervecceveviced cbastsossnsecctaasapnetaiaeedbdbesenbadtenrtenopnetiensecbcnsnes 4 3 How to change your passwords and user attributes eccsssssscssssssssssesscsscccsssesssnesees 44 How to Connect to the WAVE BiOre ctor occceecccccsssssssssssssssssssscssesseecesseeeseceeseseseesssseesssssessssessssse 4 5 How to back Up and restore system dota cecssecusesssssccsssssssssusesssessesssssssssusssessessecsscsesssnssseesees A6 Howto setup APrinter ca sassssesssaceccacscsssgiescctvsnssesecccnsansgenseccasslonsgntznnscecongaquiasestanennssencsseasqusgogatesaitononne Files and folders in UNICORN DAQ 5 1 How to create folders on ceecessssssssssessssssesssssessssssssssssessssssesssssssssstissstiassnntusstssustssnisnemeseneeess 5 2 HOW to open and preview files scscsssssssssssssrvassanssosccoisccvesvssaizeissssccecasetarsisiasdianssetecerestsriaaiaantes atv 5 3 How to arrange and locate your files ss 5 4 Howto copy delete rename and backup files ANd folders oo eeccccccssssssssssseeseeseecsseesens How to monitor and acquire data from cell cultivation runs 0000 6 1 How to start the cell Cultivation data ACQUISITION oo eecssssssssesssssecssesssssssssesesesssesssssnss
89. eted UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 127 7 How to view results 7 2 How to use the File Navigator How to set preferences for Recent Runs The File Navigator will display Recent Runs based on the individual user preference settings The table below describes how to adjust the preference settings Step Action 1 e Click the Preferences button Result The Preferences dialog box opens xi Maximum number of files to keep fi 0 Maximum no of days to keep files jo Files created by the current user only Files created by the specified users Specify All accessible files MV Remove files when saved Cancel Help e Type the maximum number of files to keep on the list e Type the maximum number of days to keep the files on the list Select which files to display on the list e Only files created by the current user e All files created by specified users Note Click the Specify button to open a dialog box and select from a list with all accessible users e All accessible files regardless of the creator e Choose Remove files when saved if the files are to be removed from the list when they have been saved e Click the OK button Result All new results will be displayed on the Recent Runs list based on the changed preferences 128 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 2 How to use the File Navigator How t
90. etwork installations log on to the network and check that you have access to the server disk and folder where the UNICORN network components are to be installed 2 e Insert the CD ROM disk into the CD ROM drive The UNICORN DAQ Setup Program should start automatically If not e click the Windows Start button and select Run e type the command d setup where d is the unit for your CD ROM drive e click OK 3 The UNICORN DAQ Setup Program is launched Continue the setup below Step 2 Install prerequisite applications Before the actual UNICORN DAQ installation can begin a number of prerequisite appli cations must be installed if they are not previously installed The applications are available in the folder SSetupPrerequistes on the installation CD but the installation will proceed automatically for each required application The applications are listed in the table below Note Some of these applications will require a restart of the computer before the installations can proceed This is noted in the table 50 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ When installing on Then the following applications are required Windows XP e Microsoft Visual C 2008 Redistributable Package x86 e OPC Core Components Redistributable x86 101 2 e Windows Installer 4 5 for Windows xP SP2 and later x86
91. f t wakes up while packed in a camying case 6 e Choose the Power Management tab e uncheck the Allow the computer to turn off this device to save power option and e click the OK button 7 Close all dialogs 66 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 General system operations Introduction This chapter describes how to start the program assign user properties and set up the system In this chapter This chapter contains these sections Section See page 4 1 Log on routines and log off routines 68 4 2 How to create a new user 72 4 3 How to change your passwords and user attributes 75 4 4 How to connect to the WAVE Bioreactor 78 4 5 How to back up and restore system data 79 4 6 How to set up a printer 82 67 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 1 Log on routines and log off routines 4 1 Log on routines and log off routines Introduction This section describes how to start and quit the UNICORN DAQ program and how to log on and log off Username and password Normally the system administrator defines the users and creates your first password The program can also be set up so you can log on without a password Note The first time after UNICORN DAQ has been installed you need to log on as a default user and create a user profile This process is described in Section 4 2 How to
92. f the actual monitor Default value is the shortest possible time between samples 188 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 10 Error reporting 10 Error reporting Introduction This chapter describes the error reporting functions In this chapter This chapter contains these sections Section See page 10 1 How to generate problem reports 190 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 189 10 Error reporting 10 1 How to generate problem reports 10 1 How to generate problem reports Introduction UNICORN DAQ contains a Generate Report Wizard for registration of errors or problems that you have detected or that occur during a run The Generate Report Wizard takes you through the steps to generate your report There are two ways of accessing the Generate Report Wizard e From the UNICORN Manager e From the System Control In this section This section contains these sub sections Section See page 10 1 1 How to generate a problem report from the UNICORN Manager 191 10 1 2 How to generate a problem report from the System Control 195 190 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 10 Error reporting 10 1 How to generate problem reports 10 1 1 How to generate a problem report from the UNICORN Manager 1011 How to generate a problem report from the UNICORN Manager Introduction The Generate Report Wizard is used to generate
93. ger opens e Choose File Printer Setup Result The Print Setup dialog box opens UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report Step Action 3 e Select an Adobe Acrobat printer from the Printer Name list e g Acrobat Distiller e Click the Properties button and edit the document properties if needed e Select the appropriate paper size and orientation e Click OK 4 e Click the Evaluation icon on the Windows taskbar Result The Evaluation module opens 5 e Print the report as described in How to print the report Result The report is created as a PDF file and saved in the location specified in your Acrobat settings Note You must have a full installation of Adobe Acrobat or a suitable printer driver to be able to do this How to save the report format The table below describes how to save the finished report format Step Action 1 e Choose File Save or e Click the Save icon Result The Save Report Format dialog box opens 2 e Type a name for the format e Select if you want to save the format for global use e Select if you want to save the format as default Note The name for the default format will automatically be changed to DEFAULT UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 165 7 How to view results 7 6 How to create and print reports
94. he System Control Note This option cannot be selected if more than one type of WAVE Bioreactor instruments will be connected 3 Click the Next button to continue Step 7 Perform the installation The wizard is ready to perform the installation If the settings are correct click the Install button to copy the files Tip If you want to make any changes you can click the Back button one or more times UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 55 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ ie UNICORN DAO 1 0 Workstation InstallShield Wizard Ready to Install the Program The wizard is ready to begin installation Click Install to begin the installation If you want to review or change any of your installation settings click Back Click Cancel to exit the wizard InstallShield Note During the installation traffic will be enabled on Port TCP 139 through the Windows Firewall A warning dialog may be shown and must be accepted before the installation can be completed Step 8 Installation completed The software installation wizard is now completed Click the Finish button to close the wizard and re start the computer Tip Select the Launch Define System Wizard checkbox to proceed with system definitions as the next step 56 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installat
95. he View Documentation icon Result the Documentation dialog box is displayed 2 e Select the Result Information tab e Select the Snapshots sub tab Result The recorded Snapshot information for a chromatogram is displayed in a list 5 You can e select other chromatograms in the Select chromatogram drop down box e select the Rows or Columns radio button to display each Snapshot as a row or a column UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 35 36 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 6 Snapshots Step Action 4 To print the Snapshot information e click the Print button e select the Snapshot check box in the Print dialog box e click OK 5 Click OK or the Cancel button to exit the Documentation dialog box UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 3 Quick Start Guide 2 3 Quick Start Guide Introduction This guide is intended as a condensed instruction on how to use UNICORN DAQ 1 0 for WAVE Bioreactor and the supplied template method to monitor and acquire data from a cell cultivation from start and ending with the optional creation of a summary report The instructions assume that all installations were made according to the instructions that the WAVE Bioreactor system is defined and is connected Quick Start instructions The table below describes the easiest way to start data collection from the WAV
96. hoose curve name appearance 137 The Curve Style and Color tab description 139 Chromatograms Description 131 How to add annota tions 173 How to add a second Y ax is 142 How to apply a layout 145 How to change and fix the X axis 143 How to change and fix the Y axis 142 How to change the size of logbook marks 147 How to edit annotation text 173 How to make layout changes general 136 How to print active chro matograms 148 How to rename 175 How to save a layout 144 Chromatogram window How to display a vertical marker 133 How to optimize the workspace 133 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Index Shortcut menu 133 Connect to the system Connecting to the Converter 100 40 Converter 100 How to connect 40 Curves Curve settings 188 Default curve names 137 Export options 178 How to add a second Y ax is 142 How to apply a layout 145 How to change and fix the X axis 143 How to change and fix the Y axis 142 How to change the color and style 139 How to choose the Y axis scale 133 How to delete unwanted curves 184 How to export 179 How to filter logbook infor mation 140 How to import 177 How to rename 175 How to save a layout 144 How to set a hatched back ground 140 How to use the zoom func tion 146 Logbook text alignment op tions 139 Monitor signals stored as curves 187 Run curves default appear ance 132 Curves pane in System Control
97. ht pane Search for topic using text search Click the Search tab Type one or more keywords you want help on in the keyword text box Result Anumber of topics containing the keywords are listed Select a match and click the Display button Result The associated help text is displayed in the right pane You can also click the Contents tab to view the contents of the Help file divided into sections Click the plus signs to expand the tree structure Click a topic to read the associated help text Context sensitive help In each dialog box there is a Help button If you press that button either of the following will be displayed e Amessage box with relevant information for example the dialog box options e The Help file with relevant information displayed in the right pane UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 31 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 6 Snapshots 2 2 6 Snapshots Introduction A Snapshot provides information about a cell cultivation run at a certain point in time It contains monitor values at the selected point Snapshot functionality is available in e the System Control module where you can take Snapshots during a run using the Marker e the Evaluation module where you can take Snapshots from a result file using the Marker How to take Snapshots during a cell cultivation run 32 The table below describes how to take Snapshots
98. if the text should appear in the same position on all pages for example as header and footer text 3 e Click the Font button to change the default font Result The Font dialog box opens e Make the necessary changes and click OK to return 4 Click OK Result The text object is inserted onto the page 156 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report How to add a picture The Picture dialog box is useful to insert logos pictures or other image objects in the report The table below describes how to add a picture object to the report Step Action 1 e Press and hold the left mouse button on the report page and drag out a box to the size of the picture item Release the mouse button e Click the Picture icon a Result The Picture dialog box opens 2 e Click the Browse button to locate the desired picture file e Select the picture file and click the Open button Note The file formats bmp emf jpg and tif can be used Result A preview of the selected picture is displayed 3 Select the desired Settings and click OK Result The picture is inserted onto the page UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 157 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report How to add a chromatogram The table bel
99. ins these topics Section See page 7 6 1 How to create and print a customized report 151 7 6 2 How to edit an existing report format 167 150 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report 7 6 1 Howto create and print a customized report Introduction You can choose from a variety of objects to include in a report including chromatograms documentation free text and more in the customized report interface You can also place align and size the objects as you please This section describes how to create and save a customized report format The saved report format can be used as a standard format for subsequent reports Saved report formats can be edited and saved as alternative report layouts This is de scribed in Section 7 6 2 How to edit an existing report format on page 167 Should you need to store store your reports in an electronic format you can save them as PDF files This section also describes how to do this How to open the Report Editor in edit mode The table below describes how to open the Report Editor in Edit mode to create a cus tomized report format Step Action 1 Open a result file in the Evaluation module 2 e Select File Report or e Click the Report icon Cl Result The Generate Report dialog box opens 3 e Click the New button Result The Create New
100. ion and system definition 3 2 2 How to install UNICORN DAQ ie UNICORN DAO 1 0 Workstation InstallShield Wizard InstallShield Wizard Completed _ The InstallShield Wizard has successfully installed UNICORN DAQ 1 0 Workstation Click Finish to exit the wizard Note Port TCP 139 is enabled in the Windows Firewall To enable communication between UNICORN software and an instrument a system needs to be defined To define a system make sure that this computer is connected to at least one instrument E Launch Define System Wizard The computer will be restarted for the configuration changes to take effect Step 9 Define system The Define System wizard is displayed if you chose Launch Define System Wizard in the previous step Tip If you choose not to define a system at this point you can start the wizard and define a system at a later time The wizard is started from the Windows Start Programs menu by clicking e GE Healthcare UNICORN DAQ Define system The table below describes how to define a WAVE Bioreactor system that is directly con nected to the PC UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 57 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ Step Action 1 The first wizard dialog shows the number of System Control modules that have been selected in a previous installation step e Click the Change button to set up another number of S
101. it was intended e You need to have a general understanding of how your PC and Windows works In most cases universal computer functions will not be explained e UNICORN DAQ must be installed and configured correctly on your computer This is described in Chapter 3 Installation on page 39 e You need to understand the concepts of cell cultivation Terminology and function alities will be explained only when they differ from normal practise e Before you try to operate a WAVE Bioreactor system you need to study and under stand the safety information that is part of the system documentation UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 1 2 UNICORN DAQ concepts 2 UNICORN DAQ concepts Introduction This chapter contains e Definitions and descriptions of some of the specific concepts that are presented in this manual e Anoverview of the UNICORN DAQ user interface Note General concepts and common cell culture terminology are not ex plained here In this chapter This chapter contains these sections Section See page 2 1 Concept definitions 13 2 2 The UNICORN DAQ user interface 15 2 3 Quick Start Guide 37 12 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 1 Concept definitions 2 1 Concept definitions Introduction This chapter contains explanations and definitions of a number of UNICORN DAQ concepts that are used in this manual The con
102. k Renew now on a Friday a new file will be started immediately and another file will be started the following Friday Click the Close button How to back up audit trail files The table below describes how to back up audit trail files Note Step Old audit trail file are automatically saved each time a new one is cre ated This backup function is only necessary if you want to copy or move the audit trail files to a specific location Action 206 Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is displayed UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA A Audit trails Step Action 2 Choose File Backup Result The Backup log files dialog box is displayed Backup log files Log files Select a folder for the files 22010031200 T E My Documente 2010030300 My Computer 2010022200 REY My Network Places 2010020900 Recycle Bin 2010011300 42009122200 2009110200 Mode O Copy Move Help You can choose either to copy or move the log files e Select the files you want to back up in the Log files field e Select a destination folder in the structure tree e Select to Copy or Move the file s in the Mode field The Move alternative is recommended to save disk space e Click the Copy or Move button depending on the previous choice e Click the Close button Note Backup operations are recorded in the audit trail UNICORN DAQ 1
103. l files Step Action oh Choose Administration Audit Trail in the UNICORN Manager Result The Audit Trail dialog box is opened see the illustration in The Global tab below By default the Global tab is displayed which shows the information of the global audit trail file If you want to examine the audit trail file for a system click a System tab There is one tab for each installed system See illustration in The System tab below Select the check boxes for the items you want to display Note All items are recorded in the audit trail The check boxes only control which items are displayed in the dialog box The Global tab The illustration below shows the Global tab of the Audit Trail dialog box 200 BE Audit Trail File Edit view Help Global BF wave 03 26 2010 16 23 33 Global log started on computer SEHC88YDP2JL 03 26 2010 16 23 50 User default logged on at SEHC8SYDPAIL User 03 26 2010 16 23 54 User default at computer SEHC88YDP2IL is in Control made of system Wave Backup 03 26 2010 16 23 54 User default at computer SEHC88YDP2JL connected to system Wave 03 26 2010 16 38 04 User default at computer SEHC88YDP2IL is in View mode of system Wave Logon Logoff 03 26 2010 16 38 04 User default at computer SEHC88YDP2JL disconnected from system Wave Define system 03 26 2010 16 38 15 User default at computer SEHC88YDP2JL is in Control mode of system Wave 03 26 2010
104. ld Description Find what Type the text string you want to find Match whole word only Select the check box if you only want complete string matches not partial matches Match case Select the check box if you only want matches which correspond according to upper case and lower case let ters Search from top of Select the check box to start the search from the top of document the document otherwise the search will start from the cursor position Direction Choose whether to search upwards or downwards in the document Commands Use the commands below to find more occurrences of a text string after you have found the first one e Press F3 to search for the next occurrence of the string or right click and choose Find next e Right click and choose Find previous to search for a previous occurrence 28 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 4 Search functions General information about searches e The default setting is to search in all result files or chromatograms e User entered search filters to a maximum of 10 will be saved in the drop down menus for both Result and Chromatogram selections More than one string can be used as a search delimiter insert between strings and search filters are automat ically saved and stored within user profiles e Click All to return to the default setting
105. ll selections 3 e If desired change the Fonts e Select if the documentation should start on a new page e If Select All Run summary or Logbook was selected make the necessary changes to the Base and Logbook filter settings 4 Click OK Result The selected documentation items are inserted into the report 160 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA How to add the Evaluation Log 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report The table below describes how to add the Evaluation Log to the report Step Action 1 e Click the Evaluation Log icon ig e Press and hold the left mouse button on the report page and drag out a box to the size of the item Release the mouse button Result The Setup Evaluation Log dialog box opens 2 e If desired change the Fonts e Select if the Evaluation Log should start on a new page 3 Click OK Result The Evaluation Log is inserted into the report How to move and resize objects freely The table below describes how to select move and resize objects freely If you want to select a single object e click the Select icon N e click the object of interest to select several objects e click the Select icon e press and hold the Ctrl key while you click the objects to move the selected objectls click on the objects hold down the left mouse b
106. lt The current system status is represented by the colored dot e A green dot represents a running system e A white dot represents a system in an End state UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Toolbar icons in the System Control 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 2 The System Control module The table below describes the toolbar icons in the module Icon Function Run The Run icon starts the data acquisition from an ongoing cell cultivation run Note The Run icon can be used only if e atemplate method has been chosen and used for a previous run using the menu command File Instant Run or short cut command ctrl l and e UNICORN DAQ has not been closed since the previous acquisition was ended This is described further in Section 6 1 How to start the cell cultivation data acquisition on page 96 End The End icon terminates the method execution and puts the system into an End state a The Customize Panes icon opens the Customize Panes dialog box which is used to select the display panes that are open D The View Documentation icon opens the documentation pages Run notes can be entered in the Notes page and settings can be changed EE The View Properties icon opens the Properties dialog box which is used to control the data display in the System Control panes UNICORN DAQ 1 0 User Manual for WAVE Bioreactor
107. ly harmful programs from making changes to your computer Tell me more abo count rol n Always notify Default Notify me only when programs try to make changes to my computer Don t notify me when I make changes to Windows settings Recommended if you use familiar programs and visit familiar websites Never notify 48 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ 3 2 2 Howto install UNICORN DAQ Installation prerequisites Before you start the installation procedure the following prerequisites have to be met e The operating system Windows XP with service pack 3 or Windows 7 must be correctly installed on your computer See the operating system documentation for details e For network installations of UNICORN DAQ the network must be correctly set up Installation notes Also notice the following e Perform the UNICORN installation procedure on each computer in the network for a network installation e Awarning message is issued if you install UNICORN to an existing UNICORN server It is not possible for the Setup program to check which UNICORN version is installed on the server so the warning is general Warning You are installing UNICORN to an existing UNICORN server on the network All computers connected to the same UNICORN server on the network have to be of the same v
108. ly if the network communication fails Electronic Signa Result files can be signed electronically for enhanced security tures and accountability 10 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 1 Introducing UNICORN DAQ 1 2 About this manual 1 2 About this manual Document structure The manual is divided into chapters Each chapter starts with a brief overview that presents the contents and the headings for the sections that the chapter contains Most sections begin with an introduction that summarizes the content Some sections are divided into sub sections A section is divided into blocks of information with separating lines The blocks are identified by a label in the margin This makes it easier for you to quickly scan a page to find the exact topic you are looking for Typographical representations Menu commands field names and other text items from the software are quoted exactly as they appear on the screen in a bold italic typeface Example Run Search paths are shown in a bold italic typeface with a separating colon between each level Example View Panes Customize i e the menu command Customize in the sub menu Panes from the View menu Text entries that UNICORN DAQ generates or that the user must type is represented by a monotype typeface Example Connection change Prerequisites The following prerequisites must be fulfilled before you can use this manual the way
109. mize the presentation of a chromatogram 135 7 5 How to print active chromatograms 148 7 6 How to create and print reports 150 7 7 Run documentation 169 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 121 7 How to view results 7 1 How to open a result file 7 1 Howto open a result file Introduction All contents of the result files are opened in the Evaluation module By default the curve data is presented as chromatograms which are shown as opened windows The chro matogram window on top is the active window See Section 7 3 Basic presentation of chromatograms on page 130 for further information about chromatograms Note It is not possible to open the same result file from two different locations simultaneously How to open a result from the UNICORN Manager To open a result file from the UNICORN Manager do one of the following e Double click a result file in the Results window of the UNICORN Manager or e Select a result file icon in the Results window of the UNICORN Manager and select File Open or e Click the Evaluation icon in the UNICORN Manager open the Evaluation module and select a result file from the Open Result dialog box How to open a result in the Evaluation module To open a result file in the Evaluation module e Do the following Select File Open Selecta result file from the Open Result dialog box or 122 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA
110. ne Description 110 Stretch to fit screen 110 Folders How to create a user specif ic 84 G Generate Report Wizard How to generate a report from the UNICORN Manag er 191 L Logbook How to filter the informa tion 140 Logbook pane Autoscroll function 111 Description 111 How to filter the con tents 112 Search function 112 Log on and log off routines How to log on 69 How to start the pro gram 68 Log off alternatives 70 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Index Quit UNICORN after log R ff 71 on Rename files and folders 93 M Reports Edit mode toolbar but Maintenance tons 152 How to use the Generate How to add documenta Report Wizard from the tion 160 System Control 195 How to use the Generate Report Wizard from the How to add free text 156 How to add objects to a re ort 155 UNICORN Manager 191 le i How to add or delete Manual instructions pages 153 Add a Set_Mark instruc f How to add picture ob tion 117 i Manual instructions in System jects 157 4 acu ay How to add the Evaluation Control og 161 Create an external value How to change the page curve 116 setup 153 Functions of buttons 118 How to save results manual ly 118 Method runs Curves pane in System Control module 105 Flow Scheme pane descrip How to create a blank cus tomized report 151 How to edit a report for mot 167 How to include chro matog
111. ne style Logbook text alignment settings The Logbook text can be set to the following alignment options e Vertical e Horizontal e Fly Over which sets text labels as hidden text that appears only when the cursor is carefully positioned over the logbook entry How to change the color and style of a curve The table below describes how to change the color and style of a curve Step Action 1 Open a result file 2 Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed 3 Click the Curve Style and Color tab e Select the curve you want to change from the list e Select the desired color and style UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 139 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 3 The Curve Style and Color tab How to display and filter logbook information The table below describes how to display and filter logbook curve information Step Action i 2 Open a result file e Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed e Click the Curve tab e Select the logbook curve e Click the Curve Style and Color tab e Click the Filter button in the Logbook text alignment field Result The Filter Logbook dialog box is displayed e Select the type of logbook information you want to show e Set the maximum block depth to show e Click OK How
112. nter and edit text in the ChromMmatogram oaceeecccsssssssssesessessccsssssssssssseessssccssssesssssssees 173 8 2 HOW to rename chromatograms ANA CUPVES wecseeessssscssscsssssssssesessesscccssssssssunseesssssessssessssussees 175 8 3 How to import and export results ov eeeecsssccccssssssssssssessessecssssssssusssessssecseessssssssseesesseeeceesssessseeees 176 8 3 1 HoW t iMpOrt reS ltS isc cossccsccnssasethcnbosscosconsteotslsessonccedsessetCensaithdqnsenoneecsnshssssostdsabaystbssedienssibossseaseo 177 83 2 Howto export results srisiimunsierianriiii ntin EEE 178 8 4 How to sign results electronically ov eecccccscsssssssssusseesssssccssssssssssssessssesssssessssssssssessesscsssssssssesnees 182 8 5 How to save results and exit the Evaluation module ou eeessessesssssssssssessssssnesssneeesneesees 184 System Settings cicxiccatereccuicceshidvaseuis desea aaaea a caesarean Nees 186 9 1 CUVE S ci ssassscsesctssccsttsessecenubayscsuccxnpvecbbensssonnsvvacdcsupepvnescgcouss AVVERRANNO NSt ass enhapanecseccternevalecsuesubaduyecoteuccbonns 187 Error reporting einari aaia aA a AEEA cai ERA 189 10 1 How to generate problem reoite 190 10 1 1 Howto generate a problem report from the UNICORN Manager ecsssssssssssssssssssssseeeees 191 10 1 2 Howto generate a problem report from the System Control ssssssssssssscccssssssssssseessee 195 AUA ONS secs eee ee 199 Data SG NAD sossen sE eects etl E ETS 208 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA
113. o close the File Navigator To close the File Navigator e Click the small cross in the top right hand corner of the File Navigator Result The File Navigator closes UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 129 7 How to view results 7 3 Basic presentation of chromatograms 7 3 Basic presentation of chromatograms Introduction This section describes how to access result files and optimize the presentation of a chromatogram and its curves via the Chromatogram Layout dialog box In this section This section contains these topics Section See page 7 3 1 Introduction to chromatograms 131 7 3 2 The chromatogram window 132 130 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 3 Basic presentation of chromatograms 7 3 1 Introduction to chromatograms 7 3 1 Introduction to chromatograms Contents of a chromatogram Chromatograms can be viewed in the Evaluation module A chromatogram includes a number of curves that have been acquired during a cell cultivation run such as temperatures pH logbook entries etc A chromatogram also contains the curves created and saved during an evaluation session The original raw data curves cannot be deleted or modified but they can be used as the basis for evalu ation procedures and subsequent creation of new curves UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 131 7 How to view results 7 3 Basic pre
114. of the acquired data in the System Control module during a cell cultivation run Step Action 1 A data acquisition is running and the System Control is displayed e Right click in the Curves pane and select Marker in the menu Result A vertical line is displayed 2 Click the marker line and drag it to the desired point where you want to take a Snapshot UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 6 Snapshots Step Action 3 Right click in the Curves pane and select Snapshot in the menu Result The Snapshot is displayed in the Snap Shot dialog box Retention h 199 43 199 43 199 43 199 43 199 43 199 43 199 43 199 43 Save to File Print Close Help Click the Save to File button if you want to save the information as an Excel file xIs or a tabbed text file txt You can also copy the information to the clipboard Click and drag the mouse in the table to select the information you want to copy Press CTRL C The information can now be pasted in a text editor Click the Print button if you want to print the information Click the Close button 5 Repeat steps 2 to 4 if you want to view more Snapshots UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 33 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 6 Snapshots How to take Snapshots in the Evaluation mod
115. ontrol unit will continue to collect data All modules will normally open when the pro gram is started However a user profile may be set up so that not all modules are available Help functions An online help utility is included in the UNICORN DAQ software The table below describes how to access the help utili ty If you want to access Then the general help utility open the Help menu in any of the software modules context specific help topics e click the Help button in the dialog box or e press the F1 key on your keyboard UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 9 1 Introducing UNICORN DAQ 1 1 About UNICORN DAQ Security The table below describes the main security functions in UNICORN DAQ Feature Function Access Security Only authorized users can access UNICORN DAQ Each user is assigned an access level which defines the functions that the user is permitted to use Connection Securi The data collection from a running cell culture instrument sys ty tem can only be controlled from one connection Systems may be locked with a password to prevent other un authorized users from terminating the data acquisition or entering manual instructions in the run log Data Security Result files from an ongoing cell culture run can be saved auto matically at preset intervals to minimize data loss for example in case of a power failure The results are saved local
116. our user name e You can also copy files to and from the folders that you have access to on the net work If you copy a folder you will also at the same time copy all files and folders that it contains The table below describes how to copy files and folders Note Follow the same steps but select Move to move files and folders Step Action 1 Select one or more files and folders in the Results window of the UNICORN Manager 2 e Select File Copy or e Right click and select Copy from the short cut menu Result The Copy dialog box is opened 3 Select a target folder or USB memory 4 Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 91 5 Files and folders in UNICORN DAQ 5 4 How to copy delete rename and backup files and folders The function Copy to External Use the function Copy to External when you need to copy files and folders outside of your own user folders for example to a USB memory How to Copy to External The table below describes how to use the function Copy to External Step Action ib 2 Select the file you want to copy e Select File Copy to External or e Right click and select Copy to External from the shortcut menu Result The Copy to External dialog box opens Select the destination drive and folder Click the Save button The function Copy from External The function Copy from External can be used to import files and folders If the files we
117. ow describes how to add a chromatogram to the report Step Action 1 e Click the Chromatogram icon e Press and hold the left mouse button on the report page and drag out a box to the size of the chromatogram Release the mouse button Result The Setup Chromatogram dialog box opens Setup Chromatogram Selected chromatogram s Active chromatogram v Settings Fonts C Thick lines C Landscape C Start on new page C Full page C Original file name Layout C Current 2 Select which chromatogram s to insert from the Selected chromatogram s droplist e Active chromatogram inserts the chromatogram that currently is active in the Evaluation module e All chromatograms inserts all chromatograms that are open in the Evaluation module e 1 2 etc inserts the corresponding chromatogram 158 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report Step Action 3 e Select the desired Settings e If desired change the Fonts Note Separate fonts can be selected for the Chromatogram and the Header text 4 e Click the Define button in the Layout field if you want to re define the layout of the chromatogram Result The Report Chromatogram Layout dialog box opens e Make the appropriate changes and click OK to return to the Setup Chromatogram dialog box Note The changes that you make will only
118. owing the options you have chosen If several systerns were selected all will be set up and the options are shown in the summary ad Define System You have successfully performed the following actions System Control Windows number updated from 1 to 4 Installed strategies are Wave_20 50_V127 Wave_200 1000_V116 Following system are modified Control Unit 1 System 1 on Wave_20 50_V127 Users default Press Finish to exit the wizard Note Ifyou installed more than one system make a note of which system is connected to which control unit This information will be useful when you set up the system table or if you must reinstall UNICORN in the future 7 When all systems that are connected to the station have been defined click the Finish button Note At this point the computer will automatically reboot A warning is displayed prompting you to shut down all other applications before acknowledging the warning When the warning is acknowledged the reboot will be performed UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 61 3 Installation 3 2 Software installation and system definition 3 2 3 Windows 7 post installation settings 3 2 3 Windows 7 post installation settings Introduction Power save settings in the default Windows 7 Power Options may interfer with the performance of UNICORN DAQ It is essential to ensure that Windows will not put either the computer or the computer network interface c
119. p to the bottom e Power LED On steady light e PC LED Flashing e System LED Flashing Connect the mains power and start the WAVE Bioreactor system Connect the mains power and start the PC UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 41 3 Installation 3 1 How to install the Converter 100 The LEDs on the Converter 100 There are three LEDs on the rear panel of the Converter 100 which can be in three dif ferent states e Off no light e Flashing with different flash rates e On steady light The table below describes what the state of each LED means LED off Flashing On Power Power off Power on BIT running or BIT not OK Power on BIT OK PC Power off Power on no PC communication Contact with PC es tablished System Power off Power on Contact with sys e Flash rate 2 Hz No contact with tems established WAVE Bioreactorsystem e Flash rate 0 5 Hz No contact with Profibus system e Flash rate 1 Hz No contact with both WAVE Bioreactor and Profibus systems BIT Built in test Note The system LED will flash while the system is scanning to identify all components At the same time the run data instruments in the System Control module will read Scanning When the scanning process is completed the system LED will stop flashing and the instruments will read Ready If any instrument component causes an error or is missing this will be indicated in the UNI
120. pplied to all chromatograms on the Evaluation workspace select the Apply to all chromatograms checkbox 4 Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 145 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 6 How to show part of a curve 7 4 6 How to show part of a curve Introduction You can select a part of a curve in order to examine details more closely You can e use the zoom to magnify or e cut the axes It is also possible to fix the axes see Section 7 4 4 How to change and fix the axes on page 142 How to use the zoom function In the active chromatogram window you can zoom in on a designated area of the chromatogram This is the easiest and quickest way to enlarge different parts of a curve The table below describes how to do this Step Action 1 Open a result file 2 e Place the mouse pointer in any corner of the area you want to magnify e Press and hold the left mouse button A magnifying glass icon will be added to the mouse pointer arrow on the screen e Drag a box to cover the area to be magnified and release the mouse button Result The selected region is now displayed in the entire chromatogram window together with appropriate scales for the Y and X axes 3 Use the arrow keys on the keyboard to move around in the chromatogram at the current zoom scale 4 Undo zoom Right click in the window and select Undo zoom to undo the last
121. r assistance 44 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 Software installation and system definition Introduction The instructions in this section describes how to install the UNICORN DAQ software and how to define a WAVE Bioreactor system during the installation Note If the system is connected to the network and installed to support re mote data acquisition make sure that the same version of UNICORN DAQ is installed on all stations in the network In this section This section contains this topic Section See page 3 2 1 Preparations before installing UNICORN DAQ 46 3 2 2 How to install UNICORN DAQ 49 3 2 3 Windows 7 post installation settings 62 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 45 3 Installation 3 2 Software installation and system definition 3 2 1 Preparations before installing UNICORN DAQ 3 2 1 Preparations before installing UNICORN DAQ User Account Control on Windows 7 The User Account Control UAC feature in Windows 7 may cause problems during the UNICORN DAQ installation If this is the case the installation may work if the UAC is temporarily disabled However it is recommended that the UAC is restored immediately after the installation and system setup is completed Disable UAC The table below describes how to disable the Windows 7 User Account Control feature Step Action
122. r interface 2 2 1 UNICORN Manager The Results window contains all the saved results and all the result folders Results Name jill Verification fed batch 20L bag001 i Test 1001 i Metod1003 i Metod1002 i Metod1001 D default Ee ji 100hRun018 Lu 100hRund17 Size Type Modified Prev Folder i verifiering 2L Batch 10 L Cyrodex 3134 Result File 2010 06 22 10 34 ut 1380 Result File 2010 07 27 14 47 1475 Result File 2010 04 16 16 26 2052 Result File 2010 07 19 17 23 2147KB Result File 2010 07 08 12 49 463KB Result File 2010 07 06 16 45 319KB Result File 2010 06 28 10 44 319KB Result File 2010 06 24 16 01 Toolbar icons in the UNICORN Manager The table below describes the toolbar icons in the module Icon Function of The Logon Logoff icon is used to log on or log off the system Note The arrow in the Logoff icon points away from the door Ee The Instant Run icon immediately starts data acquisition from a cell culti vation run using a selected template method The New Method icon is not active for UNICORN DAQ For other UNICORN applications this icon opens the Method Editor module and displays the New Method dialog box Note The Method Editor is not available for UNICORN DAQ 1 0 for WAVE Bioreactor Template methods are supplied with the installation UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82
123. rams 158 How to include Method ob tion 110 jects 159 Logbook pane descrip Howto print 164 tion 111 How to save the report for Run Data pane descrip mat 165 tion 103 How to save the report in Start from System Con PDF format 164 trol 96 Toolbar icons in Report Edit Methods Mode 162 How to export 180 Result files p Electronic signature 182 How to open in the Evalua tion module 122 How to open in the File Problem reports How to use the Generate Report Wizard from the Navigator 85 System Control 195 How to open in the UNI How to use the Generate CORN Manager 85 Report Wizard from the How to open in UNICORN UNICORN Manager 191 Manager 122 Q How to save 184 Run Data pane Quick View Description 103 How to preview result How to change text color or files 86 background 104 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 211 Index How to change the appear ance 103 S Searches General functions 27 Security Backup 94 Snapshots How to view 32 Software installation Disable network interface card power settings 64 Disable Windows 7 power save settings 62 How to install UNICORN basic instruction 50 Prerequisites for UNICORN DAQ installation 49 Prerequisite software appli cations 50 System Control module Description 20 How to customize the panes 102 How to save manual re sults 118 How to select the displayed panes 102 Manual instructions 116
124. re saved using the function Copy to External they will automatically be decompressed How to use Copy from External The table below describes how to use the function Copy from External Step Action i 2 92 Select a destination folder in the Results window e Select File Copy from External or e Right click and select Copy from External Note Do not select a file icon Result The Copy from External dialog box opens UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Step 5 Files and folders in UNICORN DAQ 5 4 How to copy delete rename and backup files and folders Action Select the files you want to copy Click Save Result e Result files are copied into the designated folder in the Results window How to rename files and folders The table below describes how to rename files and folders in the Results window in the UNICORN Manager module Step Action dL Select the item that you want to rename 2 e Select File Rename or e Right click and select Rename from the shortcut menu Result The Rename dialog box opens 3 Type a new name 4 Click OK How to delete files and folders The table below describes how to delete files and folders in the Results window in the UNICORN Manager module Note Home folders cannot be deleted this way Step Action 1 Select the item that you want to delete UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 93 5 Fil
125. s enclosed with the unit Note A network switch may be used if the computer is equipped with a single network interface card which is to be used both for the Converter 100 and the network connection A network switch is also used if several Converter 100 are to be connected to a single UNICORN DAQ computer NOTICE Make sure that the UNICORN DAQ computer all Converter 100 units and all other components are disconnected from the mains power before making the connections described below Step Action 1 Connect an Ethernet cable from the Ethernet socket on the Converter 100 to the network interface card on the UNICORN DAQ computer 40 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Step 3 Installation 3 1 How to install the Converter 100 Action Connect the WAVE Bioreactor base unit or WAVEPOD II to the appropriate Converter 100 port as shown in the table below Port Connection Primary WAVEPOD II WAVE Bioreactor 20 50 WAVE Bioreactor 200 WAVE Bioreactor500 1000 Secondary WAVEPOD II Second unit when a dual configuration is used Input 12 24V LSA Eo O Primary Secondary IP 1001 1001 1001 1001 Control no _ Az Z ce ee See SE leatsicors We Sleen G Connect the power adaptor cable to the Converter 100 connect the mains and wait until the LEDs on the rear panel show that the Converter 100 has started The LEDs should show from the to
126. scaled curve to be similarly scaled Note The values will only be applied to existing curves They will not be applied to new curves created after this function was last used How to add a second Y axis The table below describes how to add a second Y axis to the chromatogram Step Action 1 Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed 2 Click the Y Axis tab e Select the appropriate curve from the Right Axis droplist e Click the OK button 142 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 4 How to change and fix the axes How to change and fix the X axis The table below describes how to change and fix the X axis Step Action 1 Open a result file 2 Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed S Click the X Axis tab e Click the Fixed option in the Axis scale field to set the axis limits manu ally e Type the desired minimum and maximum values e Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 143 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 5 How to save and apply a layout 7 4 5 How to save and apply a layout Introduction All configurations that you make in the Chromatogram Layout dialog box can be saved as a layout It is possible to appl
127. se the sleep and display settings that you want your computer to use Q Tum oft he display 9 remecomputeo seer Mew Change advanced power settings Restore default settings for this plan UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 63 3 Installation 3 2 Software installation and system definition 3 2 3 Windows 7 post installation settings Step Action 6 Close the Control Panel Disable the network interface card power save setting The table below describes how to disable the network interface card power save settings Step Action 1 e Click the Windows Start button and e Choose the Control Panel 2 Click the Network and Sharing Center heading Result The Network and Sharing Center opens mrm SS All Control Panel Items Network and Sharing Center oa 5 Search Control Panel p Ce aiheanadba niara d ae j Control Panel Home i z e View your basic network information and set up connections I Change adapter settings ww Io o See full map ashan porr aivaocei Saa SQA 003 Network Intemet gs s This computer View your active networks Connect or disconnect J Network Access type Internet Work network Connections Local Ares Connection Change your networking settings Set up a new connection or network Set up a wireless broadband dial up ad hoc or VPN connection or set up a router or access point Connect to a network Connect or reconnect to a wireless wire
128. seesees 6 2 How to set up UNICORN DAQ to monitor the cell cultivation i 6 2 1 How to Customize System Control panes eececcsesssssssssssssssssssssssssssssssssssssssssssccssssssssssuseessssseseee 622 The R n Data PNE sis s sczaseecascsrccsssivsssaasszocceebustaia E A EAR 6 253 The Curves Pa N ecsasesddssacaessncocsnslasaasbehennenennnssnscenedeisrdsdunsvnined esgedetastentisbutues scecedsonscevbssbsansydiaecceee 6 24 Th Flow Scheme DGC sssecaucessasyascisseqlancsccsiasceetenbssensicchsveienistacconsasssvevbvtsdentdsccssopedebsestaivbentehestsens 625 The Logbook pahe isimyeisneinaninennianienn en a a UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Table of Contents 10 63 Man dl instructions stcsccoekcoasisasiiatinnnshaitauunuhsasiinuannnianmaniauhnaniann 113 6 3 1 The toolbar and status bar 114 6 3 2 Manual instructions 116 63 3 WOMINGS darunane enn REN aT E ditaa Ei 119 6 4 If the network connection FAIS ce ccccesscssssescssssescsssseesssssusessssutecsssetesssseuessseussssuusesssuesessseee 120 HONTO yIW NOS cc cocoons etic ee eee eee 121 Tek HOW tO OPEN FESUE filesinin a AAAA N NS 122 7 2 How to USE the File Navigator oaceescccccssssssssessessscccccssssssssusssssesccsssssssssussssssessecssssssssassusssssseseeeesssess 124 7 3 BaSic presentation of Chromatograms aeceeccscccssssssssssssesesesssssssssssssesesssssessssssssseussssesessesssses 130 7 3 1 Introduction to Chromatograms wieee
129. sentation of chromatograms 7 3 2 The chromatogram window 7 3 2 The chromatogram window Main views The chromatogram window is divided into two main views e File Navigator e curves The displayed areas for the views can be adjusted by dragging the borders with the mouse cursor between the views inminiirrurrairrt af PIE Biehl th wo we Wo we wo wo Run curves default appearance and information The first time a result file is opened and viewed a default layout is applied to display all the original curves The default layout can be changed by the user see Section 7 4 5 How to save and apply a layout on page 144 Information for each curve Each curve is automatically assigned a default color and style with default information about each curve displayed in the key above the curves This information includes e result file name e chromatogram name 132 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 3 Basic presentation of chromatograms 7 3 2 The chromatogram window e curve name Choose the Y axis scale Each curve has a correspondingly colored Y axis To choose the appropriate Y axis scale e click on the Y axis until the desired scale is displayed or e click on the name of the curve Run curves shortcut menu When viewing curves in the Evaluation module you can access a menu that provides a quick alternative to menu commands Right click the run curves view
130. stem e Click the OK button to close the dialog and proceed Result The selected settings are shown in the System settings field of the wizard page gt Define System System Installation Set system parameters for a control unit Select a Control Unit for a system and press the Change button to edit the settings When all systems physically connected to the computer have been defined press the Install button to continue Select Control Unit for System Control Unit Control Unit2 Control Unit3 Control Unit 4 System settings System name System 1 SystemT ype Wave Strategy name Wave_20 50_V127 Controller CU 960 Advanced IP address 192 168 668 1 lt Back 5 If there is more than one system to define repeat steps 3 to 4 for each sys tem Note By default the Converter 100 controllers are set up to be 60 used as Control Unit 1 If you set up more systems than one you will need Converter 100 units which have been reset to the correct control unit number by GE Healthcare field services The Converter 100 control unit number is written on the IP address label on the rear panel of the Converter 100 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ Step Action 6 Click the Install button Result The system is set up with the selected settings and a Summary page is displayed sh
131. subsequent sections in this chapter The table below describes how to make changes in the Chromatogram Layout dialog box Step Action 1 Open a result file 2 e Right click the chromatogram window and select Properties or e Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed The view from which you activate the Properties command determines the tab that is displayed in the Chromatogram Layout dialog box 3 Carry out the changes on the different tabs to get the desired layout for header and curves Select Apply to all chromatograms if you want to apply changes made in the Chromatogram Layout dialog box to all open chromatograms Click OK 136 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 2 The Curve tab and Curve Names tab 7 4 2 The Curve tab and Curve Names tab The Curve tab The Curve tab of the Chromatogram Layout dialog box contains a list of all the curves included in the chromatogram Select the curves you want to display in the chro matogram and click OK Curve name appearance You select options for the curve name appearance on the Curve Names tab This is an example of a default curve name Result 11_Rate The table below describes the three components that make up the default curve name Component Description Example Result name Name of the result Fed b
132. tallShield Wizard Destination Folder Click Next to install to this Folder or click Change to install to a different fol Install UNICORN DAQ 1 0 Workstation to CAUNICORN Follow the instructions in the table to select another destination folder Step Action 1 Click the Change button to open a browse dialog 2 Browse to and select the destination folder where the program is to be in stalled This should be on a physical disk drive on the computer where you install UNICORN DAQ not on a network disk drive e Click the OK button to continue e Click the Yes button if asked whether Setup should create the UNICORN program folder UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ Step 5 Setup Type The Setup Type dialog box is displayed ie UNICORN DAO 1 0 Workstation InstallShield Wizard Setup Type Choose the setup type that best suits your needs Select the type of installation you prefer then press Next The network installation will setup the software to work over a network Check Remote only system if no systems will be physically connected to the computer 4 remote only system have to be installed to an existing UNICORN server The stand alone installation will setup the software to work locally on the target system Setup Type Network installation Remote only system connect
133. tasks 46 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 1 Preparations before installing UNICORN DAQ Step Action 4 e Click and drag the slider control down to the position Never notify and e Click the OK button iy ccount Conti ings Es fog x Choose when to be notified about changes to your computer User Account Control helps prevent potentially harmful programs from making changes to your computer Tell me more about User Account Control settings Always notify Never notify me when Programs try to install software or make changes to my computer a J Imake changes to Windows settings Not recommended Choose this only if you need to use programs that are not certified for Windows 7 0 because they do not support User Account Control Never notify 5 e Acknowledge the change in the pop up dialog e close the Control Panel and e proceed with the UNICORN DAQ installation UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 47 3 Installation 3 2 Software installation and system definition 3 2 1 Preparations before installing UNICORN DAQ Restore UAC After the UNICORN DAQ installation is completed follow the instruction above again this time to restore the UAC setting to the default state Choose when to be notified about changes to your computer User Account Control helps prevent potential
134. the Name field Select a file type from the Type drop down box Select if the search should include subfolders Select date limits in the Date drop down boxes Type text strings to match Question or Answer texts Type a variable name and if desired a value Type a Batch ID Click Find Result The search results are listed in the Found folders and files field The search is limited to the folder including its subfolders that is currently dis played Double click a file in this list Result The dialog box is closed and the selected file is highlighted in the UNICORN Manager window Note Ifyou click Close you will return to the UNICORN Manager window with no file highlighted regardless if you have se lected one in the dialog box or not UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 5 Files and folders in UNICORN DAQ 5 4 How to copy delete rename and backup files and folders 5 4 Howto copy delete rename and backup files and folders Introduction UNICORN DAQ has some file and folder handling functions that are slightly different from the general Windows functions This section focuses on the differences Note You need explicit authorization in your user profile to copy move and delete files How to copy or move files and folders There are some restrictions to how you can copy or move files and folders e Files and folders can only be copied or moved to folders that are specific to y
135. the UNICORN Manager e The result files and folders are listed in the Results window e You can only see folders that you have access to How to create a user specific The table below describes how to create a user specific folder Action Select the Results window you want Result The window title bar is highlighted e Select File New Folder or e Right click and select the New Folder shortcut Result The Create New Folder dialog box opens Type a name for the new folder Click OK folder Step i 2 3 4 84 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 5 Files and folders in UNICORN DAQ 5 2 How to open and preview files 5 2 Howto open and preview files Introduction This section describes how to open your saved result files You can also preview your result files to identify the correct file before you open it How to open a result file in UNICORN Manager You can open a result file in the UNICORN Manager module Click the file in the Results window to select it and e choose File Open or e right click the file and choose Open from the short cut menu or e double click the file Result The file is opened for editing in the Evaluation module How to open a result file in the Evaluation module The table below describes how to open a result file from the File Navigator in the Eval uation module Step Action 1 e Click the Files tab UNICORN DAQ 1 0 User
136. tion 4 1 Log on routines and log off routines on page 68 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 19 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 2 The System Control module 2 2 2 The System Control module Introduction The System Control module is used to monitor and start data collection from cell culti vation runs The System Control panes The System Control module contains four different display panes that can be opened all at once or in any combination The Run Data pane The Curves pane The Flow Scheme pane The Logbook pane The Run Data pane The Run Data pane displays the current values for the selected run parameters The values are updated at regular intervals which are defined in the system strategy See the illustration below 20 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 2 The System Control module The Curves pane The Curves pane displays monitor signal values graphically See the illustration below Rate t_Airflow L_Temp L_02 L_pH C L00 Net_Weight ae 23 S88 lt a few feeu seve fees sewed leva lessnuwe ceeeewwe buwe hues swan fy od d A d o OONA GOGU gt off set 1 L f Oo N PORE PEPE EEL EEE PE PEEL TT eee h 05 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 4 The Flow Scheme pan
137. to display a hatched background The table below describes how to display a hatched background in the chromatogram window Step Action 1 2 140 Open a result file e Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 3 The Curve Style and Color tab Step Action 3 e Click the Curve Style and Color tab e Select the Hatch box e If desired select the Apply to all chromatograms box and click OK Result Hatch marks are displayed as a background Note You can also right click in the Chromatogram window and select Hatch UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 141 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 4 How to change and fix the axes 7 4 4 How to change and fix the axes How to change and fix the Y axis The table below describes how to change and fix the Y axis Step Action 1 Open a result file 2 Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed 3 Click the Y Axis tab e Select the appropriate curve from the list e Click the Fixed option 5 e Type the desired minimum and maximum values e Click the All with this unit button if you want other curves with the same Y axis units as the current
138. to existing server Server disk Stand alone installation Installshield The table below describes how to select the setup type Step Action 1 You can perform either a Stand alone installation or a Network installation More information about the network option is shown in Network Installation below Note Ifyou perform a stand alone installation and later want to connect the system to a network you must remove the current installation and install the software with the appro priate settings 2 When you have made your selections click the Next button to continue Network installation A network installation can be either e alocal station UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 53 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ or e aremote only system You have to select these disk drives in a network installation e Alocal disk for the program files This was determined in step 4 when the destination folder was selected e Aserver disk for the server files Select the Remote only system check box to install a remote only system that being a computer to which no systems are physically connected Note When you perform a network installation the necessary UNICORN software components will be copied automatically to the network server disk Step 6 Program Settings Follow the instructions in the table below to set the req
139. to monitor and acquire data from cell cultivation runs 6 3 Manual instructions 6 3 Manual instructions Introduction This section describes how to control the data acquisition with manual commands and instructions In this section This section contains these topics Section See page 6 3 1 The toolbar and status bar 114 6 3 2 Manual instructions 116 6 3 3 Warnings 119 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 113 6 How to monitor and acquire data from cell cultivation runs 6 3 Manual instructions 6 3 1 The toolbar and status bar 6 3 1 The toolbar and status bar Toolbar buttons The toolbar at the top of the System Control module contains three sets of buttons e Manual Direct Commands buttons for starting and stopping the run e Windows buttons to access dialog boxes for pane selection documentation and layout properties e System Access buttons to control the system connection Show and hide The toolbars can be shown and hidden by choosing View Toolbars and selecting the relevant boxes The figure below shows the toolbar Run End Pr VS Direct command button functions 114 The table below describes the functions of the Manual direct command buttons Button Function Run Starts a data acquisition using the template method that was used for the previous data acquisition Note This button only works properly ifa data acquisition already has been performed on the comp
140. to operate The system specific part is usually referred to as the Strategy The Strategy defines available manual instructions system settings run data curves and method templates Template A Template is a basic method that is used as a starting point for developing runs for data acquisition Warnings A Warning dialog may be displayed on the screen if an alarm is triggered by WAVE Bioreactor This Warning must be acknowledged to close the dialog The WAVE Bioreactor system will still continue the run and data will continue to be collected by UNICORN DAQ while the Warning is displayed The event causing the Warning will be included in the log 14 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 The UNICORN DAQ user interface Introduction This section is an overview of the three UNICORN DAQ modules with descriptions of some of the elements of the user interface The section also contains a description of the search functions in UNICORN DAQ Note A user profile can be set up so that the user only has limited access to the modules described in this chapter Only the available modules will open when the program is started In this section This section contains these sub sections Section See page 2 2 1 UNICORN Manager 16 2 2 2 The System Control module 20 2 2 3 The Evaluation module 25 2 2 4 Search functions 27 2 2 5 Help functions 30 2 2 6
141. to set up UNICORN DAQ to monitor the cell cultivation 6 2 3 The Curves pane Step Action 1 Select View Properties Result The Properties dialog box is displayed 2 Select the Curve Style and Color tab 3 e Select a curve from the Curve list e Select an appropriate color and style How to change the scale of the Y axis In most cases the Y axis is automatically scaled for each of the curves Values on the Y axis apply to the curve with the same color as the axis markings To get the correct Y axis click the legend The table below describes how to fix the scale of individual curves Step Action 1 e Select View Properties Result The Properties dialog box is displayed e Select the Y axis tab 2 e Select the appropriate curve e Select Fixed and type a minimum and maximum range in the fields within the specified limits 3 Repeat step 2 for other curves if needed 4 Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 107 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 3 The Curves pane How to change the scale of the X axis The table below describes how to change the scale of the X axis Step Action 1 e Select View Properties Result The Properties dialog box is displayed e Select the X axis tab 2 Select the appropriate Axis scale e Total will show the curves as far as they have come in the run e Windo
142. tograms on the default Windows printer Step Action cl Open all chromatograms that you want to print in the Evaluation module 148 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 5 How to print active chromatograms Step Action 2 e Select File Print or e Click the Print toolbar icon Result The Print Chromatograms dialog box opens 5 Select print format and layout options 4 e Click OK to print or e Proceed with step 5 to preview and edit the layout 5 Click the Preview button Result The Customize Report window opens 6 e Click the Edit Mode button to make changes e g change the order of the chromatograms see Section 7 6 1 How to create and print a cus tomized report on page 151 for more information about how to edit e Click the Preview button to return to preview mode 7 e Select File Print or e Click the Print toolbar icon Result The Print dialog box opens 8 e Select the print range and number of copies e Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 149 7 How to view results 7 6 How to create and print reports 7 6 How to create and print reports Introduction The Evaluation module provides extensive tools to create detailed reports This section describes how to create and print reports that are based either on a customized layout or a previously saved format In this section This section conta
143. tration Create Restore Backup in the UNICORN Manager to display the Create Restore Backup dialog box Create Restore Backup Action Create O Restore Browse Items Global Files Global Report Formats Personal Files cr System Files User Setup System Setup 3 e Inthe Action field make sure that the Create option is selected e Click the Browse button to select where to store the backup file e Inthe Items field select which information to include on the backup file e Click the Create button to create the backup file and store it in the se lected location Note You can click the Information button to see which informa tion files will be included in the backup file 80 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 5 How to back up and restore system data How to restore the system data The table below describes how to restore the system data from a backup file located for example on a USB memory Step Action 1 If the backup file is located on a USB memory insert this into the computer 2 Choose Administration Create Restore Backup in the UNICORN Manager to display the Create Restore Backup dialog box Create Restore Backup Action O Create Restore Browse Items Global Files Global Report Formats Personal Files g System Files User Setup setup In the Action field select the Restore option
144. ubject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare repre sentative for the most current information UNICORN Any use of this software is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9846 82 AA 11 2010
145. uired parameters for password and system control windows ie UNICORN DAO 1 0 Workstation InstallShield Wizard Program Settings Choose the program settings that best suits your needs Set the requirement for minimum number of password characters and the System Control layout Press Next button to continue Password Password required Minimum number of password characters O No password required System Control Layout Standard The number of System Control Windows O Advanced InstallShield 54 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ Step Action 1 Select Password required and type the minimum number of characters re quired for passwords Valid numbers of password characters are 3 15 Select No password required if you do not require password protection With this setting users can be defined with or without passwords Note Make sure that you enter the same password settings on each station in a network 2 Choose the number of System Control Windows that should be available in the installation Maximum value is 4 or Choose the Advanced layout option for System Control where up to four WAVE Bioreactor systems can be monitored from a single System Control window One system is viewed at a time The system that is viewed in the Advanced layout is selected from a sidebar in t
146. ule The table below describes how to take Snapshots in the Evaluation module Step Action 1 e Open a result file in the Evaluation module e Right click and select Marker in the menu Result A vertical line indicating a certain point is displayed 2 Click the marker line and drag it to the desired point where you want to take a Snapshot 3 Right click and select Snapshot in the menu Result The Snapshot is displayed in the Snap Shot dialog box 199 43 199 43 199 43 199 43 199 43 199 43 199 43 34 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 6 Snapshots Step Action 4 e Click the Save to File button if you want to save the information as an Excel file xls or a tabbed text file txt e Youcan also copy the information to the clipboard Click and drag the mouse in the table to select the information you want to copy Press CTRL C The information can now be pasted in a text editor e Click the Print button if you want to print the information e Click the Close button 5 Repeat steps 2 to 4 if you want to view more Snapshots How to view recorded Snapshots The table below describes how to view Snapshots which have been recorded during a method run using the Snapshot text instruction Step Action 1 In the Evaluation module e choose View Documentation or e click t
147. ule displays up to four panes for monitoring different aspects of the run To select what panes to display either e click the Customize Panes icon or e choose View Panes How to customize System Control panes Change the size Select a split bar and drag up and down to change the size of a specific pane Maximize restore or hide Right click a pane and select the appropriate option to e maximize e restore or e hide the pane 102 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 2 The Run Data pane 6 2 2 The Run Data pane Description The Run Data pane displays the current values for selected run parameters The update interval is defined in the system strategy The figure below displays an example of the Run Data pane How to change the appearance of the pane The appearance of the pane can be changed so that it includes more or fewer data displays The table below describes how this is done Step Action 1 e In System Control select View Properties or e right click on the pane and select Properties on the menu Result The Properties dialog box is displayed UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 103 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 2 The
148. ults 8 3 How to import and export results 8 3 1 How to import results 8 3 1 Howto import results Introduction This section describes how to import curves in different formats Curve formats You can only import curves in ASCII text format How to import curves The table below describes how to import curves Step Action 1 Choose File Import Curve Result A menu with the available curve formats opens 2 Choose the correct curve format Result The Choose File to Import From dialog box opens 5 Locate the file that contains the curve and double click the file Result The Import Curves dialog box opens 4 Select the curve s to import and click the OK button Result The curves are opened in the Evaluation module UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 177 8 How to edit results 8 3 How to import and export results 8 3 2 How to export results 8 3 2 How to export results Introduction This section describes how to export curves in different formats and how to copy data and curves to the clipboard Data formats You can export data in the following formats e ASCII asc e Excel xls Export options Select File Export in the Evaluation module to export data from an open result file The following export options are available e Curves e Documentation e Evaluation log 178 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA How to export
149. user If there are no keyboard entries or mouse movements within the time limit the workstation will be locked or logged off Note A locked workstation can be activated again only by the previous user if the regular log in password is entered If another user wants to log on and use the workstation the previous user can be logged off without entering the correct password The previous user s files will be closed and the new user will only have access to his own files UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 4 General system operations 4 1 Log on routines and log off routines Systems locked by other users You can unlock a system that has been locked by another user if you have the correct password You may still be able to unlock a system even if you do not have the password Any user with Unlock locked systems authorization can override another user s lock by entering his or her own logon password However it is recommended that this authorization is limited to only a few users How to quit UNICORN DAQ UNICORN DAQ will still be open after you have logged off To close the program you must log in again and quit UNICORN DAQ you cannot quit the program if you are not logged in The table below describes how to do this Step Action 1 e Select the File Quit Program menu command in the UNICORN Manager module or e Click the close icon in the top right hand corner of the program window Result A
150. uter The first data acquisi tion must be started by choosing the File instant Run menu command End Terminates the run data acquisition Note You can choose to save a partial result or discard it Note The commands can also be found on the Manual menu UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 3 Manual instructions 6 3 1 The toolbar and status bar Windows buttons The table below describes the functions of the Windows buttons Button Function Opens a dialog box where you can choose which window an panes to display This button is equivalent to the menu command View Panes Opens the documentation pages Run notes can be en tered on the Notes tab and settings can be changed Other tabs are displayed for information only This button is equivalent to the menu command View Documentation Note Notes entered here about the data ac quisition in progress will not be included in the Logbook Opens the properties pages This button is equivalent to the menu command View Properties Status bar connection status The status bar displays a message indicating the connection status of the window The table below describes the different messages Message Connection status Controlled by lt user gt The indicated user has a control mode connection to the software Other users can establish a
151. utton and drag the objectis to the new position UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 161 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report If you want to resize the selected click one of the object border anchors either in the corners object s or in the middle of a border and drag the box to the new size Note Some Text objects cannot be resized Alignment toolbar icon functions 162 Objects can be placed in exact positions and sized in relation to other objects The table below describes the function of the Alignment toolbar icons in the Report Editor Toolbar Function icon Align left Es Matches the left alignment of all selected objects to that of the highlighted object Align right Matches the right alignment of all selected objects to that of the highlighted object Align top Matches the top alignment of all selected objects to that of the highlighted object Align bottom Matches the bottom alignment of all selected objects to that of the highlight ed object Adjust to margins Stretches the selected object s to the left and right margins UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 6 How to create and print reports 7 6 1 How to create and print a customized report Function Adjust to left margin Adjusts the selected object s to the left margin
152. w or e Right click and choose Quick View from the short cut menu Result The Quick View dialog box opens 3 e Click the Next and Previous buttons to move between the result files if more than one is selected Result The result file that is displayed in the dialog box opens in the Evalua tion module UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 87 5 Files and folders in UNICORN DAQ 5 3 How to arrange and locate your files 5 3 How to arrange and locate your files Introduction This section describes how to arrange the way the files are displayed in your UNICORN DAQ workspace and how to locate files through a search Different view modes You can choose how the files and folders are displayed in the UNICORN Manager win dows The options are the standard Windows alternatives e Details e List e Large icons Small icons How to change the view mode If you want to change the view you either e Select View and the option that you want or e Right click and select View and the option that you want from the shortcut menu Sort order in detailed view The files can be sorted in a different order when a window is displayed in detailed view The table below shows the options Sorted by Order Name Alphabetical order or reverse alphabetical order Size Smallest or largest files first Type Alphabetical order of file extension type Modified Most recently modified files first
153. w allows you to set the portion of the total pane to be displayed e Click OK How to zoom in the Curves pane The table below describes how to zoom in on a selected region of the curve pane Step Action 1 e Press and hold the left mouse button and drag a rectangle out on the screen to encompass the area to be viewed e Release the mouse button Result The display is now zoomed in on the selected area 2 Repeat the process for further magnification of selected areas How to zoom out To reduce the scale of the zoom right click in the Curves pane and select one of the following options e Undo Zoom reverses each zoom in action a step at a time e Reset Zoom reverses all zoom in actions to the default scale 108 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 6 How to monitor and acquire data from cell cultivation runs 6 2 How to set up UNICORN DAQ to monitor the cell cultivation 6 2 3 The Curves pane How to edit text in the Curves pane You can select the way that text is aligned for the Logbook curves You can also select to show only part of the Logbook information The table below describes how to do this Step Action 1 Right click in the Curves pane and select Properties in the displayed menu Result The Properties dialog box is displayed 2 Select the Curve Style and Color tab 3 Select the following e Logbook curve in the Curve list as appropriate e Select the appropriat
154. xport file format from the Save as type droplist e ASCII files asc e Excel files xls Select a destination folder Type a file name and click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 179 8 How to edit results 8 3 How to import and export results 8 3 2 How to export results Note Curves are exported as series of numerical coordinates that refers to the time volume and signal respectively How to limit the exported curves You can optimize the exported curves to only the parts that you want to focus on in the Export Curves dialog box The table below describes how to use these editing options Dialog box option Instruction Cut curves Enter retention values in the text boxes to limit the curve to only a portion of the original curve Cut graphically This button opens the Export Cut dialog box Move the vertical markers to the correct cutoff points Reduce number of Adjust the factor value or the maximum number of sam samples ples To reduce the number of samples by a factor of five means that only every fifth point will be sampled for export Normalize retention Select the Normalize retention checkbox to have all ex ported curves normalized to a common x axis How to export methods documentation and evaluation logs The table below shows how to export methods documentation and evaluation logs Step Action i Select the data you want to export 2 e Select
155. y To select another folder click the Browse button and open the desired folder Search the Result list e The search will take place in all result files within the selected folder as denoted by the asterisk To select specific result file s click the Browse button and select the result file s e You can use wildcard characters to search for chromatograms within result files with a specific name profile represents any number of characters represents any single character Wildcard character examples xyz will search files named xyz xyz will search all files with names that begin with xyz xyz will search all files with names that end with xyz xyz will search only 4 character names that end with xyz Search the Chromatogram list The asterisk indicates that all chromatograms within a result file will be selected Click Browse to select one or several specific chromatograms UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 27 2 UNICORN DAQ concepts 2 2 The UNICORN DAQ user interface 2 2 4 Search functions Search the Curve name list The curves are identified by number To search for all pH curves select pH in the Curve name text field Find a text string The Find command is used to search for text strings Find x Find what HK I Match whole word only T Match case I Search from top of document m Direction c i C Up sies Down Fie
156. y saved layouts to other chromatograms All saved layouts are user specific How to save a layout The table below describes how to save a layout Step Action il Open a result file 2 Choose Edit Chromatogram Layout Result The Chromatogram Layout dialog box is displayed 3 Make the appropriate layout configuration within the various tabs View your changes Click OK if you want to return to the chromatogram window to see the ap plied affects of a given configuration Return to the Chromatogram Layout dialog box to perform further changes 4 e Select the Layout Library tab e Click the Save current layout as button Result The Save Layout dialog box is displayed 5 e Type a name for the layout e If you want the current layout to be the new default layout select the Save as default option e Click OK Result The new name is added to the Saved layouts list 6 Click OK 144 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 5 How to save and apply a layout How to apply a layout The table below describes how to apply a layout Step Action 1 Select the Layout Library tab on the Chromatogram Layout dialog box 2 e Select a layout from the Saved layouts list e Click the Apply selected layout button Result The layout is automatically applied to the active chromatogram window 3 If the same layout is to be a
157. ystem Control System Control Layout Select the number of System Control Windows e Note This option is not available if the Advanced layout was selected for System Control Click Next to proceed 2 e Select the appropriate strategy for the WAVE Bioreactor system in the Strategies field and e Click the Install button b Define System Select Strategy Select the strategy to install Select the strategy you want to install and press Install button If your strategy is located on a CD or on another source press the Have Disk button to locate it When all strategies are installed press Next to continue 2 strategies found on D Settings F V127 Wave_200 1000_V116 lt Back Next gt J Cancel 58 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA Step Action 3 Installation 3 2 Software installation and system definition 3 2 2 How to install UNICORN DAQ Note If the strategy is not listed in the Strategies field click the Have Disc button and browse to locate the strategy file on the strategy CD Select the strategy folder and click OK to add the strategy to the wizard field You will be asked to enter a name for the strategy before it is added to the list At this point you can install all strategies that you will need if you plan to set up several different WAVE Bioreactor system types The button View Installed Strategies will open a list of all strategi
158. zoom step Reset zoom Right click in the window and select Reset zoom to reset all zoom steps at once 146 UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 7 How to view results 7 4 How to optimize the presentation of a chromatogram 7 4 7 How to change the size of the Logbook marks 7 4 7 How to change the size of the Logbook marks Introduction The sizes of the Logbook marks are all determined by your user settings The settings are applied for all your chromatograms Instruction The table below describes how to change the size of the Logbook marks Step Action 1 e Choose Administration Change User Attributes in the UNICORN Man ager module Result The Change user attributes dialog box opens 2 Select the unit for the Logbook mark height e Percent of window height e Character Heights e Pixels Type a new size value in the Logbook mark height box 3 Click OK UNICORN DAQ 1 0 User Manual for WAVE Bioreactor 28 9846 82 AA 147 7 How to view results 7 5 How to print active chromatograms 7 5 How to print active chromatograms Introduction This section describes how to print the chromatograms that are open in the Evaluation module The Print Chromatograms dialog box This is an illustration of the Print Chromatograms dialog box Note The selected print format is outlined in red Print Chromatograms aa Instruction The table below describes how to print active chroma

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UNICORN™ DAQ 1.0 - GE Healthcare Life Sciences