BG-Power Supply
Contents
1. g A Pr ERE Pam N AN a AYN VA v v Vi rr N a a WVA r NS DD A BG Power Supply Installation and Operation Manual BG Power300 BG Power600 BG Power600 P O Box 48488 Roosevelt Park 2129 South Africa Tel Jhb 011 475 1823 Dbn 031 301 7617 CT 021461 9607 Fax 011475 1819 Email sales vacutec co za SEES ABIES Dre EP EO oa MACUTEC TE PARS Table of Contents SO EEE 1 Section 1 Introduction rrurrurrurvarnnrnarvnrnnvnnrenernenneenerneenueneenurnuenuenunnnsnnee 2 SEE EE E E a 2 1 2 Delivery FP ACK AG Cis ceca ccconatncnduenentcescius semesiemoteendedddascteceuedensetess 2 Section 2 Technical description arrarvarnnrrnnenrrnvrnerrernernnenernurnuenuenusnnsnne 3 2 1 Front FT 3 2 2 Rear Panel rrrrnarnarnarnernennrnnnnnrnnenernnrnannsnnsnueneenennsnnsnueneeneene 3 Zo ISCO A 0 EE E aneie 4 2 4 Output Sockets rrrrrnrrnernarrnrvnrrnrenevnvenernvrnerneennenennenusnnennsene 4 Section 3 Operation rrrrrrrurnnrrurvarrnrrarvnnrnrnnnenennenneereeneenueneenunnuenuenusnnennee 5 Pte D JLT OUCK StA EEE 5 TPM 6 3 2 RUNNING a Progra iienaa aaa 7 Section 4 Maintenance rrurnurnnrnarnnnnnnnnnnnannnenennennerneeneenuenuennenusnnsnnsnnene 8 Section 5 Trouble Shooting rrarrrnrrnrrrarerarnnrrranrranrnnnnnnrnnnnnannnernnnennnnnenn 8 QUICK START GUIDE aannnnnnnnunnnnnnnnnnnunnnnnns I BG Power Supply Manual Contents Safety Extreme caution should b2. display BG Power Supply Manual Page 8 BG Power 600 Power Supply quick start manual BE NAC UTEC S gt Turn the Power supply on A cursor will appear below the selected menu use the left amp right arrow keys to re seen Press the Enter key to select the desired menu 1 Quick Start Menu Setting voltage current or power limits The display will now display the default SET V screen for voltage adjustment Use the SCROLL keys Left amp right arrows to move the cursor to the required field then adjust the digits using the SWITCH keys Confirm with the ENTER key Repeat above procedures for the current and power priority modes Each priority mode allows specific adjustment of either voltage current or power as each is set the program will automatically calculate the resulting voltage current or power for your selection Setting time limit The default time is 999 minutes To change the time limit switch to the required digit using the SWITCH keys and select the desired values with the SCROLL keys left amp right arrows Press ENTER to Store settings Press RUN PAUSE key to run 2 Programming Menu Saving a program Choose a blank program and set the voltage current or power limit as described above Press ENTER to store program setting Press RUN PAUSE key to run The parameters can also be changed using the procedures described above and the changes will be saved
3. e exercised in the operation of this instrument as it can produce sufficient voltage and current to cause a lethal shock To avoid any risk of injury the instrument should only be operated by properly trained personnel and always in accordance with the instructions provided Read this entire manual before using this power supply 1 2 3 This instrument is for laboratory use only The instrument must always be used with the protective earth lead of the power cord correctly grounded to earth at the mains outlet To permit sufficient be sure that there is at least 6cm clearance around the power supply Do not block the fan vents at the rear of the unit Do not operate the power supply in extreme humidity gt 95 or where condensation can short the internal electrical circuits of the power supply Keep the instrument as dry and clean as possible Wipe regularly with a soft damp cloth Let the power supply dry completely before use Always unplug the power supply while cleaning it and until it is dry When taking the power supply into a cold room the unit can be operated immediately However when removing the power supply from the cold room let the unit equilibrate to room temperature for a minimum of 2 hours before using it Note that the output is connected to the chassis reference earth BG Power Supply Manual Page 1 Section 1 Introduction 1 1 Overview BG Power Supply is a high quality high precision and safe power
4. es back to the previous display 2 4 Output Sockets There are four sets of output sockets to allow four electrophoresis units to be connected and run at the same time The voltage output is 0 600V for BG Power600 and BG Power600i 0 300V for BG Power300 BG Power Supply Manual Page 4 Section 3 Operation 3 1 Programming The Programming procedure can be followed step by step in the quick reference diagram 3 on page 6 Start Position When the power supply is switched on the display shows the start mode with a cursor below the selected menu For example 1 Quick Start 2 Programming If the power supply is switched on for the first time the values of program voltage current and power are 000V the maximum current and power respectively Choosing a mode Use SWITCH KEYS to choose 1 or 2 and press ENTER KEY to confirm a mode shown on the display 3 1 1 Quick Start Setting voltage current or power limits The display will now display the default SET V screen for voltage adjustment Use the SCROLL KEYS to move the cursor to the required field then adjust the digits using the SWITCH KEYS Confirm with the ENTER KEY Repeat above procedures for the current and power priority modes Each priority mode allows specific adjustment of either voltage current or power as each is set the program will automatically calculate the resulting voltage current or power for your selection Setting time limit The default ti
5. me is 999 minutes To change the time limit switch to the required digit using the SWITCH KEYS and select the desired values with the SCROLL KEYS Press ENTER KEY to store settings Press RUN PAUSE KEY to run BG Power Supply Manual Page 5 3 1 2 Program Saving a program Choose a blank program and set the voltage current or power limit as described above Press ENTER KEY to store program setting Press RUN PAUSE KEY to run The parameters can also be changed using the procedures described above and the changes will be saved to the program 600V 005mA 300W 999min SET I current priority mode XXXV 100mA XXXW 666min RUN I XXXV 100mA XXXW 999min RUN I BG Power Supply Manual 1 Quick Start 2 Programming sam Confirm by 005V 500mA 300W 999min SET V voltage priority mode 100V XXXmA XXXW 666min RUN V 600V 500mA 005W 999min SET P XXXV XXXmA 100W 666min RUN P Diagram 3 Programming Procedure Page 6 3 2 Running a Program Connect the leads from the electrophoresis unit Red to Red and Black to Black Red is positive and Black is negative Up to four electrophoresis units can run at the same voltage at one time NOTE The maximum voltage current and power load between the four electrophoresis units cannot exceed the Rated voltage current and power of the power supply Example four units cannot run at 100V each on a 300V power supply the maximum voltage between the fo
6. s off The end parameter values are displayed and an alarm will sound Stop the alarm by pressing any KEY BG Power Supply Manual Page 7 A run cannot be continued after pressing the STOP KEY You can restart the program by pressing the RUN KEY Disconnect the leads and proceed with post electrophoretic techniques Since diffusion will begin as soon as the voltage is turned off you should remove the gel and begin staining blotting or autoradiography immediately Section 4 Maintenance Wipe the instrument regularly with a damp cloth Let the instrument dry completely before use No further user maintenance is required Should you encounter any malfunction please contact Vacutec for further service information Section 5 Trouble Shooting Error Cause Solution ERROR CODE XX The current is less than the lower Check connections and or buffers CHECK THE LOAD limit This can be due to incorrect connection of the electrophoresis equipment or due to use of buffers with extremely low conductivity ERROR CODE XX The current to ground leakage in the Check the electrophoresis unit GROUND LEAKAGE electrophoresis unit is too high ERROR CODE XX The mains voltage is out of the range 1 Check voltage selector POWER FAIL 2 Check mains voltage ERROR CODE XX If a serious error occurs the program Record the error code number CALL SERVICE enters the FAIL mode The output is and contact Vacutec switched off and an error message is shown in the
7. supply for electrophoresis applications that require a maximum voltage of 300 600 V Electrophoresis separations can be controlled by voltage current or power BG Power Supply Is primarily designed for the following speciality 1 Easy to read digital display 2 Timed or continuous runs with end of run alarm if desired 3 Automatic parameter limit crossover to prevent overheating and protect experiments and equipment 4 Membrane keypads for easy programming 2 Four pairs of power outlets for duplicate parallel runs 6 Constant voltage constant current and constant power modes T Single unit increments in settings and read outs for precision and reproducibility 8 Stores and recalls twelve protocols 9 Suitable for general applications including mini and standard sized vertical gel and many blotting applications 1 2 Delivery Package When you receive the power supply carefully inspect the container for any damage which may have occurred in shipping Severe damage to the container may indicate damage to the power supply itself If you suspect damage to the unit Please contact Vacutec immediately Contents include e BG Power Supply Power Cord User Manual If any part is missing or damaged contact Vacutec immediately BG Power Supply Manual Page 2 Section 2 Technical Description 2 1 Front Panel The front panel consists of a Liquid Crystal Display LCD a keyboard with 8 membrane keys a Light Emit
8. ting Diode LED that lights when voltage is applied HV on and connectors for electrophoresis units see Diagram 1 ER an a i 3 B I i 2 2 Rear Panel Diagram 1 The rear panel is shown in Diagram 2 below On the rear panel there is 1 A mains switch Press in I to switch on the power to the power supply Press 0 to switch off the power 2 A socket for the mains cable 3 A switch for voltage range The left position corresponds to 100 120V and the right to 200 240V 4 Vent openings BG Power Supply Manual Diagram 2 Page 3 2 3 Keyboard ENTER KEY Enters a mode locks the value if correct and moves programming to the next field SWITCH KEY oe Switch the place of the cursor and view the settings during a run SCROLL KEY Changes the value or other choice in the field of the selected parameter RUN PAUSE KEY Pressing this key starts the run and puts the program into RUN Gi mode when the instrument is out of run The program number current values for voltage current and power are shown on the display The countdown time is also displayed Pressing this key while in RUN mode puts the instrument in PAUSE mode and switches off the voltage The display shows the status of the run at the time the key was pressed STOP KEY Stops the run and puts the instrument into END mode The O voltage is switched off and the end parameters are displayed EXIT KEY exit Go
9. to the program 1 Quick Start 2 Programming Set by Confirm by 600V 005mA 300W 005V 500mA 300W 600V 500mA 005W 999min SET I 999min SET V 999min SET P current priority mode XXXV 100mA XXXW 100V XXXmA XXXW XXXV XXXMA 100W 666min RUN I 666min RUN V 666min RUN P XXXV 100mA XXXW 999min RUN I Tel Jhb 011 475 1823 CT 021 461 9607 P O Box 48488 sales vacutec co za Dbn 031 301 7617 Fax Jhb 011 475 1819 Roosevelt Park 2129 www vacutec co za
10. ur units will be 75V each Choosing a mode Press the SWITCH KEY to select 1 or 2 with the cursor and confirm your choice with the ENTER KEY Omit this step if you have already set a program as described in section 3 1 Running Press RUN KEY to start the electrophoresis The display will show current values for voltage current power and elapsed time or volt hours The HV on LED shows when voltage is applied If no current is displayed check the electrical connections to the electrophoresis equipment Pausing You can interrupt the electrophoresis for sample loading and or changing parameter values by pressing the PAUSE KEY Once voltage and current are no longer be supplied the HV on LED will switch off and you may then safely load your samples The display will show the status of the run sequence at the time the PAUSE KEY was pressed When sample loading is complete press the RUN KEY again and the run will continue from the last state prior to the interruption View programmed values It is possible to view the programmed values during a run by pressing the SWITCH KEY NOTE Values cannot be changed here The display returns automatically to show the current run values after 5 seconds Stopping the run and viewing end parameter values When the programmed time limit is reached the program will enter the end mode It is possible to interrupt the run manually by pressing the STOP KEY In both cases the HV on switche
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