Minigel-Twin user manual
Contents
1. 1 well preparative Code No 010 013 2 wells Code No 010 021 3 wells Code No 010 014 5 wells Code No 010 015 10 wells Code No 010 016 10 wells for big samples or high protein concentrations Code No 010 024 20 wells Code No 010 020 Others Silicone rubber seal 1 0 mm Code No 010 005 Silicone rubber seal 0 6 mm Code No 010 006 Clips 3 pcs Code No 010 007 biometra a part of Analytik Jena The Multigel Code No 010 200 comes complete with all the acessories needed to cast and run 11 x 7 cm w x1 gels Acessories included with this system are main chamber for vertical gels 11 x 7 cm including cooling device and safety lid Two notched glass plate with straight edges Code No 010 201 Two glass plates with fixed 1 0 mm spacers Code No 010 202 Two combs 24 wells 1 0 mm Code No 010 204 Two silicone rubber seals 1 0 mm Code No 010 205 Three sets of clips a 3 pieces Code No 010 007 One Manual The Multigel Long Code No 010 300 comes complete with all the acessories needed to cast and run 11 x 12 cm w x gels Acessories included with this system are main chamber for vertical gels 11 x 12 cm including cooling device and safety lid Two notched glass plate with straight edges Code No 010 301 Two glass plates with fixed 1 0 mm spacers Code No 010 302 Two combs 24 wells 1 0 mm Code No 010 204 Two silicone rubber seals 1 0 mm Code No 010 303 Four2. One notched glass plate with straight edges Code No 010 003 One glass plate with fixed 1 0 mm spacers Code No 010 001 One comb 10 wells 1 0 mm Code No 010 011 One silicone rubber seal 1 0 mm Code No 010 006 One sets of clips a 3 pieces Code No 010 007 One Manual The Minigel Twin Code No 010 100 comes complete with all the acessories needed to cast and run 8 6 x 7 7 cm w x l gels Acessories included with this system are main chamber for vertical gels 8 6 x 7 7 cm including cooling device and safety lid Two notched glass plates with straight edges Code No 010 003 Two glass plates with fixed 1 0 mm spacers Code No 010 001 Two combs 10 wells 1 0 mm Code No 010 011 Two silicone rubber seals 1 0 mm Code No 010 006 Two sets of clips a 3 pieces Code No 010 007 One Manual Accessories Glass plates Glass plates with fixed 1 0 mm spacers Glass plates with fixed 0 6 mm spacers Code No 010 001 Code No 010 002 Notched glass plate with straight edge Notched glass plate with inclined edge Combs 1 mm 1 well preparative 2 wells 3 wells 5 wells 10 wells 10 wells for big samples or high protein concentrations 20 wells Code No 010 003 Code No 010 004 Code No 010 008 Code No 010 022 Code No 010 009 Code No 010 010 Code No 010 01 1 Code No 010 023 Code No 010 012 biometra a part of Analytik Jena Combs 0 6 mm
3. Using the cooling option do not mix up the in and out plugs for the cooling water The connection for the water cooling with the smaller diameter is the inlet the connection with the bigger diameter is the outlet Best cooling is obtained using a refrigerated circulator chiller with a temperature of 5 C Attention Reduce flow rate to max 0 5 1 l min and use no organic solvents or alcohol Operating Conditions max V DC max mA max W max Temp Minigel 400 25 1 Gel 10 50 C Minigel Twin 400 50 2 Gele 20 50 C Multigel 400 70 2 Gele 28 50 C Multigel Long 450 70 2 Gele 32 50 C Maxigel 500 120 2 Gele 60 50 C Lengthy transfer times are not recommended Do not leave the instrument unattended Joule heat can be generated during electrophoresis Electrophoresis runs longer than 4 6 hours at max settings can damage the unit Do not use alcohol e g methanol ethanol or organic solvents for cooling or cleaning the apparatus 5 biometra a part of Analytik Jena gt gt This products are designed and certified to meet EN 61010 1 safety standards Certified products are safe to use when operated in accordance with the instruction manual This instruments should not be modified or altered in any way Alteration of this instruments will void the warranty void the EN61010 1 certification and create a potential safety hazard biometra a part of Analytik Jena 3 1 Unpack and Check
4. Unpack and carefully examine the electrophoresis unit Report any damage to BIOMETRA Do not attempt to operate this device if physical damage is present Save all packing material if damage is found I Attention A Please fill out and send back the warranty registration card This is important for you to claim to full warranty both parts and repair are covered within the full warranty period 3 2 Electricity Supply This electrophoresis chamber has been designed to operate with D C current Warning This supply must not be earthed 3 3 Important Electrical Safety Notes Do not operate this equipment if any of the following conditions exist If buffer leaks from the main electrophoresis chamber If cracks are present in the electrophoresis chamber or safety cover If the electrical connection cables are worn or frayed 4 0 Location Place the chamber in proximity to the Power Pack with which it is to be connected Be sure to place the chamber in a safe dry location away from the edge of the working surface biometra a part of Analytik Jena 5 0 Handling 5 1 Mounting the glass plates Put one silicone rubber seal between two thoroughly cleaned glass plates one glass plate with fixed spacers one notched glass plate and fix the glass plate set with 1 clip right 1 clip left and 1 clip at the bottom for Minigel and Minigel Twin 1 clip right 1 clip left and 2 clips at the bottom for Multigel 2 clips ri
5. alcohol e g methanol ethanol or organic solvents e g acetone on the electrophoresis unit A DO NOT use chromosulfuric acid to clean the glass plates A The system should never be autoclaved or placed in a microwave 10 biometra a part of Analytik Jena 6 0 Solutions for SDS Polyacrylamide Gels used 6 1 Running Gel Minigel and Minigel Twin 6 ml per gel Acrylamide conc in 75 10 0 12 5 15 0 17 5 30 acrylamide 0 8 bisacrylamide stock solution in ml 15 20 25 3 0 3 5 1 88 M Tris HCl pH 8 8 in ml 0 5 SDS in ml dist H2O in ml TEMED in ul ammonium persulphate sol 10 in ul 30 30 30 30 30 o N upm O hh O 4 ho pf o oOo hh o oOo 4 fh po Multigel 9 ml per gel Acrylamide conc in 75 10 0 12 5 15 0 17 5 30 acrylamide 0 8 bisacrylamide stock solution in ml 2 25 3 0 3 75 4 5 5 25 1 88 M Tris HCl pH 8 8 in ml 18 18 18 18 1 8 0 5 SDS in ml 18 18 18 18 18 dist H2O in ml 3 15 24 165 09 0 15 TEMED in ul 8 8 8 8 8 ammonium persulphate sol 10 in ul 45 45 45 45 45 Multigel Long 12 ml per gel Acrylamide conc in 75 10 00 12 5 15 0 17 5 30 acrylamide 0 8 bisacrylamide stock solution in ml 3 0 40 50 6 0 7 0 1 88 M Tris HCl pH 8 8 in ml 24 24 24 24 2 4 0 5 SDS in ml 24 24 24 24 2 4 dist H2O in ml 42 32 22 12 0 2 TEMED in ul 10 10 10 10 10 ammonium persulphate sol 10 in ul 60 60 60 60 60 11 biometra a part of Analytik Jena Maxigel 30 ml per
6. gel Acrylamide conc in 7 5 10 0 12 5 15 0 17 5 30 acrylamide 0 8 bisacrylamide stock solution in ml 7 5 10 0 12 5 15 0 17 5 1 88 M Tris HCI pH 8 8 in ml 60 60 60 60 60 0 5 SDS in ml 60 60 60 60 60 dist H2O in ml 105 80 55 30 0 5 TEMED in ul 25 25 25 25 25 ammonium persulphate sol 10 in ul 150 150 150 150 150 A Attention The ammonium persulphate solution 10 should be prepared fresh every day and should be stored at 4 C refrigerator 12 biometra a part of Analytik Jena 6 2 Stacking Gel 5 Minigel and Minigel Twin 2 ml per gel 0 33 ml 30 acrylamide 0 8 bisacrylamide stock solution 0 4 ml 0 625 M Tris HCl pH 6 8 0 4 ml 0 5 SDS 0 87 ml dist H2O 2 ul TEMED 10 ul 10 ammonium persulphate sol Multigel and Multigel Long 4 ml per gel 0 66 ml 30 acrylamide 0 8 bisacrylamide stock solution 0 8 ml 0 625 M Tris HCI pH 6 8 0 8 ml 0 5 SDS 1 74 ml dist H2O 4 ul TEMED 20 ul 10 ammonium persulphate sol Maxigel 6 ml per gel 1 0 ml 30 acrylamide 0 8 bisacrylamide stock solution 1 2 ml 0 625 M Tris HCI pH 6 8 1 2 ml 0 5 SDS 2 6 ml bidist H2O 6 ul TEMED 30 ul 10 ammonium persulphate sol A Attention The ammonium persulphate solution 10 should be prepared fresh every day and should be stored at 4 C refrigerator 13 biometra a part of Analytik Jena 6 3 SDS Sample Buffer for SDS PAGE gels under reducing conditions 2 ml 0 625 M Tris HCl pH 6 8 0 2g
7. 0 Code No 040 400 or Power Pack P25 Code No 040 800 from Biometra Be aware that the plus pole in red is connected to the lower buffer reservoir The following conditions can be recommended for a gel with a 10 Running Gel and 1mm spacers Minigel Minigel Twin Stacking gel 10 mA Running Separation gel 25 mA biometra a part of Analytik Jena Multigel and Multigel Long Stacking gel 10 20 mA Running Separation gel 25 35 mA Maxigel Stacking gel 15 25 mA Running Separation gel 50 60 mA The values may vary with gel concentration Mulitgel Multigel Long and Maxigel A more homogeneous run with even sharper bands can be achieved by water cooling A Do not mix up the in and out plugs for the cooling water Max flow rate unpressurized 0 5 1 l min Stop the electrophoresis when the bromphenol blue band has nearly reached the bottom of the running gel Switch off the Power Pack and open the safety lid after the plugs of the Multigel have been disconnected from the Power Pack 5 7 Staining and Destaining Slide the gel carefully from the glass plates into the Coomassie Brilliant Blue staining solution and stain it for 30 60 minutes with gentle agitation Fixation with trichloroacetic acid is not necessary Destain briefly with a solution of 45 ethanol 10 acetic acid 45 water andthen in a solution of 7 acetic acid in water 5 8 Cleaning A Use only mild non abrasive detergents DO NOT use
8. 10 417 Clips 3 pcs Code No 010 007 Maxi Clips 4 pcs Code No 010 416 biometra a part of Analytik Jena 2 0 Safety and Warning Notices 2 1 Definition of Symbols S oa 2 Definition Caution Refer to instruction manual Danger High voltage Fragile PER Using with direct current DC 2 2 General Safety Instructions AA A A A A Delicate instuments Handle with care Do not operate this equipment if buffer or water leaks from the instrument if cracks are present in the body or the safety cover or if the electrical connection cables are worn or frayed Make sure that the on off switch of the used external Power Supply is always free accessible Danger High voltage The current to the cell provided from the external Power Supply enters the unit through the lid assembly providing a safety interlock to the user Current to the cell is broken when the lid is removed Do not attempt to circumvent this safety interlock and always turn Power Supply off before removing the lid or when working with the cell in any way biometra a part of Analytik Jena Power to the instrument is supplied by an external DC voltage Power Supply The output of this Power Supply must be isolated from external ground to issue that the DC voltage output floats with respect to round All Biometra Power Supplies meet this safety requirement Never place the instrument on top of a Power Supply
9. SDS 5ml glycerol 0 5 ml B mercaptoethanol 0 1 ml bromophenol blue 1 in ethanol 2 4 ml dist H2O Dilute the sample buffer 1 to 1 with the sample 6 4 Running Buffer pH 8 3 5 liter 15 19 Tris Base 72 09 glycine 5 09 SDS 6 5 Staining Solution 1 liter 2 09 Coomassie Brilliant Blue R 250 0 5g Coomassie Brilliant Blue G 250 425 ml ethanol 50 ml methanol 100 ml acetic acid 425 ml bidest water Stirr overnight filtrate before use store in dark bottle 6 6 Destaining solution fast destaining in 45 ethanol 10 acetic acid 75 bidest water slow destaining in 25 isopropanol 10 glacial acid 45 bidest water final destaining in 7 acetic acid in bidest water 14 biometra a part of Analytik Jena Should you have any problems with this unit please contact our service department or your local Biometra dealer Biometra GmbH Service Department Rudolf Wissell StraBe 14 16 D 37079 Goettingen Phone 49 0 5 51 50686 100r12 Fax 49 0 5 51 50686 11 e mail Service biometra com A If you would like to send the unit back to us please read the following return instructions Instructions for return shipment e Return only defective devices For technical problems which are not definitively recognisable as device faults please contact the Technical Service Department at Biometra Tel 49 0 5 51 50 88 1 10 or 12 Fax 49 0 5 51 50 88 1 11 e mail Service biometra com e Please contact ou
10. a 8 0 Equipment Decontamination Certificate To enable us to comply with german law i e 71 StrlSchV 17 GefStoffV and 19 ChemG and to avoid exposure to hazardous materials during handling or repair please complete this form prior to the equipment leaving your laboratory COMPANY INSTITUTE ADDRESS PHONE NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluation Start Date Finish Date Hazardous materials used with this equipment Method of cleaning decontamination The equipment has been cleaned and decontaminated NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GMBH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER 17 biometra a part of Analytik Jena General Information for Decontamination Please contact your responsible health amp safety officer for details Use of radioactive substances Please contact your responsible person for details Use of genetically change organism or parts of those Please contact your responsible person for details biometra a part of Analytik Jena 19 biometra a part of Analytik Jena EU Declaration of Conformity G ttingen March 2009 im Sinne der EG Richtlinie ber
11. biometra a part of Analytik Jena Minigel Family Vertical Polyacrylamide Gel electrophoresis Apparatus Instruction Manual a TS Modell Order No Minigel 010 000 010 030 010 040 Minigel Twin 010 100 010 130 010 140 Multigel 010 200 010 220 010 230 Multigel Long 010 300 010 320 010 330 Maxigel 010 400 010 430 010 440 Warning A Please read these instructions carefully A before using this apparatus Biometra GmbH Service Department Rudolf Wissell Str 30 Rudolf Wissell Str 14 16 D 37079 Goettingen D 37079 Goettingen Tel 49 0 5 51 50 68 6 0 Tel 49 0 551 50 68 6 10 or 12 Fax 49 0 5 51 50 68 6 66 Fax 49 0 551 50 68 6 11 e mail Info biometra com e mail Service biometra com internet http www biometra de biometra a part of Analytik Jena This document describes the state at the time of publishing It needs not necessarily agree with future versions Subject to change Fastblot Instruction Manual 2009 11 biometra a part of Analytik Jena 1 0 Intended Uses And Specifications The Biometra Slabgels are intended to be used for the fractionation of Proteins and DNA RNA molecules in an vertical Polyacrylamid gelelectrophoresis The Minigel Code No 010 000 comes complete with all the acessories needed to cast and run 8 6 x 7 7 cm w x I gels Acessories included with this system are main chamber for vertical gels 8 6 x 7 7 cm including cooling device and safety lid
12. elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 73 23 EWG following the EC directive about electrical equipment for use within certain limits of voltage 73 23 EEC Hiermit erkl ren wir da folgende Slabgele Herewith we declare that the following Slabgel Systems Typen types Minigel Minigel Twin Multigel Multigel Long Maxigel Best Nr Order No 010 000 010 030 010 040 010 100 010 130 010 140 010 200 010 220 010 230 010 270 010 300 010 320 010 330 010 400 010 430 010 440 den grundlegenden Anforderungen der corresponds to the basic requirements of EG Niederspannungsrichtlinie 73 23 EWG entsprechen EC low voltage directive 73 23 EEC Folgende harmonisierte Normen wurden angewandt The following harmonized standards have been used EN 61 010 1 in Anlehnung corresponding to EN 61 010 1 A2 in Anlehnung corresponding to Dr J rgen Otte Quality Manager 20 biometra a part of Analytik Jena Warranty This laboratory instrument is produced with the highest practical standards of materials workmanship and design The design and manufacture of parts have been conceived with one purpose to produce units which will give satisfactory service Biometra GmbH guarantees this unit to be free from defects in materials or workmanship under normal use or service for 24 month from date of shipment If during this time this unit proves defective in materials or workma
13. ght 2 clips left and 2 clips at the bottom for Multigel Long 2 clips right 2 clips left and 2 Maxi clips at the bottom for Maxigel A Attention Acrylamide is a neurotoxin Handle gels and gel solutions with care and wear rubber gloves 5 2 Running Gel Minigel Minigel Twin About 6 ml of gel solution are needed if 1 0 mm spacers are used Immediately after addition of TEMED and ammoniumpersulfate pour the solution between the glass plates up to 4 cm and overlay it carefully with 0 2 ml of bidistilled water Multigel About 9 ml of gel solution are needed if 1 0 mm spacers are used Immediately after addition of TEMED and ammoniumpersulfate pour the solution between the glass plates up to 5 5 5 cm and overlay it carefully with 0 8 ml of bidistilled water Multigel Long About 12 ml of gel solution are needed if 1 0 mm spacers are used Immediately after addition of TEMED and ammoniumpersulfate pour the solution between the glass plates up to 9 9 5 cm and overlay it carefully with 0 8 ml of bidistilled water Maxigel About 30 ml of gel solution are needed if 1 0 mm spacers are used Immediately after addition of TEMED and ammoniumpersulfate pour the solution between the glass plates up to 16 cm from the bottom and overlay it carefully with bidistilled water Polymerisation visible by the formation of a sharp boundary between gel and overlay takes about 20 40 minutes Remove the water completely from the
14. nship Biometra GmbH will repair or replace it free of charge if returned to us prepaid This guarantee does not cover damage in transit damage caused by carelessness misuse or neglect or unsatisfactory performance as a result of conditions beyond our control or consequential losses as a result of failure of our product Rudolf Wissell Str 30 D 37079 G ttingen Postfach 1544 D 37005 G ttingen Tel 49 0 5 51 50 68 6 0 Fax 49 0 5 51 50 68 6 66 e mail Info biometra com internet http www biometra de Biometra GmbH Service Department Rudolf Wissell Str 14 16 D 37079 G ttingen Tel 49 0 5 51 50 88 1 10 or 12 Fax 49 0 5 51 50 88 1 11 e mail Service biometra com 21
15. r service department for providing a return authorization number RAN This number has to be applied clearly visible to the outer box Returns without the RAN will be not be accepted e Important Clean all parts of the instrument from residues and of biologically dangerous chemical and radioactive contaminants If the device is A contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible damages and losses resulting from insufficient decontamination of the device e Please prepare written confirmation use the Equipment Decontamination Declaration following on the next page that the device is free of biologically dangerous chemical or radioactive contaminants This confirmation must be attached to the outside of the packaging e Use the original packing or a similarly robust packing when returning the device If not available contact Biometra or your local distributor 15 biometra a part of Analytik Jena e Label the outside of the box with CAUTION SENSITIVE INSTRUMENT and the RAN number sticker Attach the Decontamination Declarartion e Please enclose a note which contains the following a Sender s name and address b Name of a contact person for further inquiries with telephone number c Precise description of the fault which also reveals during which procedures the fault occurred if possible 16 biometra a part of Analytik Jen
16. sets of clips a 3 pieces Code No 010 007 One Manual Accessories Glass plates Glass plates with fixed 1 0 mm spacers Code No 010 202 notched glass plate Code No 010 201 Combs 12 wells 1 0 mm Code No 010 203 24 wells 1 0 mm Code No 010 204 Others Silicone rubber seals 1 0 mm Code No 010 205 Clips 3 pcs Code No 010 007 12 channel pipette variable 5 50 ul volume in 0 1 ul steps Code No 010 206 biometra a part of Analytik Jena The Maxigel Code No 010 400 comes complete with all the acessories needed to cast and run 17 x 18 cm w x gels Acessories included with this system are main chamber for vertical gels 17 x 18 cm including cooling device and safety lid Two notched glass plate with straight edges Code No 010 401 Two glass plates with fixed 1 0 mm spacers Code No 010 402 Two combs 12 wells 1 0 mm Code No 010 404 Two silicone rubber seals 1 0 mm Code No 010 417 Three sets of clips a 3 pieces Code No 010 007 One set of Maxi clips a 4 pieces Code No 010 416 One Manual Accessories Glass plates Glass plates with fixed 1 0 mm spacers Code No 010 402 notched glass plate Code No 010 401 Combs 12 wells 1 0 mm Code No 010 404 18 wells 1 0 mm Code No 010 405 24 wells 1 0 mm Code No 010 406 1 well 2 marker lanes 1 0 mm preparative Code No 010 407 Others Silicone rubber seals 1 0 mm Code No 0
17. surface of the polymerised gel with a sheet of filter paper biometra a part of Analytik Jena 5 3 Stacking Gel Minigel Minigel Twin About 2 ml of stacking gel solution is required Multigel and Multigel Long About 2 5 to 3 ml of stacking gel solution is required Maxigel About 3 5 ml of stacking gel solution is required Insert the comb after pouring the solution between the glass plates The polymerisation should take about 20 30 minutes 5 4 Assembly Remove the silicone rubber seals and combs fill up the lower buffer reservoir with the running buffer and mount the plates onto the apparatus Make shure that there are no air bubbles present at the bottom of the running gel You may achieve this by tilting the plates when dipping them into the buffer After fixing the glass plate sets with the clamps onto the body of the electrophoresis apparatus fill up the upper buffer reservoir with buffer and remove the comb carefully Wash the slots with buffer 5 5 Sample Application Dissolve the sample in SDS sample buffer and carefully load it into the slots of the stacking gel If you are using samples precipitated with trichloroacetic acid neutralise them first with Tris base solution As the 24 well comb for Multigel and Multigel Long Code No 010 204 is compatible with the multichannel pipet multiple loading of samples is possible 5 6 Electrophoresis Mount the safety lid Switch on the power supply e g Power Pack P2
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