ar7 user guide - Reichert Technologies: Analytical Instruments
Contents
1. AR7 SERIES Automatic Refractometer User s Guide Reichert Analytical Instruments Preliminary Information Introduction Instrument Initialization Operation Operational Parameters Features Maintenance Specifications Accessories Warranty Table of Contents Warnings and Cautions Congratulations on your Purchase Environmental Conditions Electrical Ratings Specifications Instrument Features Set Up Set Point Calibration Multi Point Calibration Sample Reading Shadow line display Sample Temperature Effects on Readings Automatic Temperature Compensation Temperature coefficient and correction calculations Display Screen Explanation of Functions Printer Output RS232 Serial Port Serial Control Codes Channel 1 ICUMSA Brix Channels 2 15 User Programmable Key Pad Function Lock Unlock Error Messages Care and Cleaning Care of Prism Surface Preventative Maintenace Extended Precision Calibration Oils Flow Cell Funnel Cell Sample Press Warranty Terms Po NOOO CAUTION IN ORDER TO INSURE THAT CORRECT OPERATION OF THE AR7 SERIES INSTRUMENT IS MAINTAINED ANY REPAIR OR SERVICE MUST BE PERFORMED BY EXPERIENCED PERSONNEL THAT ARE TRAINED BY REICHERT INC CAUTION ESDS THE INTERNAL CIRCUITRY OF THE AR7 SERIES CONTAINS ELEC TROSTATIC DISCHARGE SENSITIVE DEVICES ESDS SUCH COMPONENTS MAY BE SENSITIVE TO HIGH VOLTAGES PRODUCED BY STATIC DISCHARGES CAUTION DO NOT USE STRONG SOLVENTS2. CALIBRATION Highlight point select with READ Repeat with additional points as needed When complete select EXIT Refractive Brix Last Index Equivalent Calibrated 1 40229 1 45755 1 51416 1 57230 N H EXIT Use U D to highlight READ to select 11 12 Clean and dry the prism and well Add sample and allow to temperature equilibrate Press READ Note value on display Use MODE key to switch between the four modes of measurement 1 INDEX Refractive index 2 INDEX TC Refractive Index Temperature Compensated 3 SOLIDS Percent Concentration 4 SOLIDS TC Percent Concentration Temperature Compensated The above modes in channel one are for measurement of Sucrose or Brix scale per the International Commission for Uniform Methods All other channels are custom scales moou gt F Compare reading to known value Note any difference in well temperature from sample reference temperature and adjust refractive index reading mathematically from the INDEX displayed value The AR7 SERIES has the ability to graphically display the shadow line enabling the user to see what the instrument sees To operate this feature after taking a reading press and hold the FUNCTION key for at least five seconds When the key is then released the AR7 SERIES will generate and display a graph of the Linear Scanned Array output To exit the shadow line display press READ Voltage output of Detector G
3. OR STRONG CLEANING SOLUTIONS ON ANY PART OF THE AR7 SERIES OR DAMAGE TO THE UNIT MAY OCCUR WARNING DO NOT REMOVE OR DEFEAT THE EARTH GROUND CONNECTION ON THE AR7 SERIES POWER INPUT CONNECTOR OR THE UNIT S POWER CORD DAMAGE TO THE AR7 SERIES INSTRUMENT AND OR INJURY TO THE OPERATOR MAY OCCUR WARNING THE AR7 SERIES SHOULD BE USED IN STRICT ACCORDANCE WITH THE INSTRUCTIONS OUTLINED IN THIS USER S GUIDE THE SAFETY OF THE OPERATOR AND THE PERFORMANCE OF THE INSTRUMENT CANNOT BE GUARANTEED IF USED INAMANNER NOT SPECIFIED BY REICHERT INC Symbol Information The following symbols appear on the instrument CAUTION Risk of electric shock AUTION Replace with same type and rating fuse 5x20 3 15A a yp g ae 250V Quick Acting N CAUTION Indicates that important operating and maintenance are included in this User s Guide Congratulations on your purchase of your new Reichert AR7 SERIES Refractometer This manual describes the proper operation and care of this state of the art instrument The AR7 SERIES is an automatic bench top refractometer with digital read out and is capable of measuring Solids Brix Solids TC Brix Temperature Compensated Sucrose Refractive Index and Refractive Index TC The Peltier controller eliminates the need for a water bath and has the greatest controllable temperature range set point available AnAR7 SERIEShas the capability to display data in four languages English Spanish Frenc
4. Setup Calibration Auto Temperature Channel Routine Equilibration Set amp Span Temperature Contrast Advanced Exit Setpoint Options L R to choose option U D to change setting To exit press READ F Allow the instrument a warm up period of at least 30 minutes before calibration or sample reading Calibration with distilled water only also referred to single point calibration A Turn the instrument on by activating the power switch located on the rear panel B Set atthe desired operating temperature in the set up screen by pressing SET UP and scrolling to Temperature Setpoint C Allow the instrument a warm up period of at least 30 minutes before calibration D Press CALIBRATE E Follow the displayed instructions to clean prism and press CALIBRATE again Clean prism and press CALIBRATE again to reinitiate or any other key to cancel Well Temp 20 01 C F Select reading mode by pressing MODE Index TC Solids TC Use MODE to select mode Current mode INDEX TC Add distilled water to sample well Allow to equilibrate then press CALIBRATE Well temp 20 01C G Apply enough distilled water to cover the the prism surface allow to temperature equilibrate and press CALIBRATE Reading and Calibrating H Water calibration complete Reported value should be 1 33299 00005 Water calibration complete 1 33299 imex rc Reference Temperature 20 C Press SETUP t
5. cable DO NOT USE a Null Modem Cable when making the connection IMPORTANT NOTE Connect any required accessories printer computer to the appropriate COM port on the instrument utilizing the included null modem cables Do not disconnect without turning off power to the instrument The AR7 SERIES is capable of being controlled from a computer through the Serial Port interface by using any standard communications program such as Terminal or Hyperterminal Reading output through the serial port may be formatted to retrieve virtually any data in any order desired To format the serial string output type help sf brief instructions will appear To set the serial format type sf followed by the letters for the output options desired The default setting is GFA Many of the advanced functions can only be accessed and set through the serial port connection For a complete list of those codes contact your technical customer service representative The AR7 SERIES is preprogrammed at the factory with channel 1 to read Sucrose or Brix scale per the International Commission for Uniform Methods of Sugar Analysis ICUMSA Temperature Corrected TC readings in channel 1 are valid for Sucrose only User programmable channels or custom channels may be programmed using your own Refractive index Temperature Solids data Access to the channels is from the Set Up screen Custom channels can include definitions for
6. displayed of the dynamic well temperature vs time C CHANNEL Default ICUMSA Channel 1 is programmed for BRIX from the International Commission for Uniform Methods of Sugar Analysis sucrose ICUMSA scale temperature compensated in TC mode to 20 C Channels 2 through 15 are user programmable channels For directions and an explanation of the necessity of custom channels please request Technical Bulletin Custom Channel Generation for Automatic Refractometer Line Notify your representative of the instruments software version displayed when first turned on as Ver XX X D TEMPERATURE SETPOINT To control the sample prism temperature highlight this selection and use the UP DWN keys to adjust E CONTRAST Once highlighted the display contrast can be adjusted by pressing and holding the up or down key until the desired contrast is attained F ADVANCED OPTIONS Selecting this option refreshes the display and offers another Setup screen with additional options INTERVAL TIMER Default OFF Set in HH MM SS format this option allows the instrument to take readings at definable intervals Once activated and defined the AR7 SERIES will continually take readings until a lock out error occurs such as a lack of sample or the option is disabled Interval Timer is set to OFF when the HH MM SS is set to 00 00 00 and it is deselected DELAY TIMER Default O SECONDS Sets the time a sample is read once t
7. need for a pumping device 13107200 includes the flow cell funnel funnel attachment and tubing Catalog No 13107300 Sample Press with Temperature Control The sample press creates an enclosed sample volume by contacting the top of sample and prism well It is useful for decreasing equilibration times of very viscous hot or cold samples It is also useful for measuring volatile liquids such as alcohols Ports are provided for circulation of a controlled temperature fluid Please refer to our web site www reichert com Analytical Products and select Terms and Conditions of Sale for an explaination of the warranty covering this Instrument The warranty period for this product is two years 23 Reichert Ophthalmic Instruments Reichert Inc 3362 Walden Ave Depew NY 14043 USA Toll Free 888 849 8955 Phone 716 686 4500 email info reichert com www reichert com Reichert GmbH Hubertusstrasse 4 D 82229 Seefeld Germany Tel 49 8152 993530 ISO 9001 Certified Reichert 2003 Reichert Inc Depew NY USA 13107000 100 Rev F
8. 0412 dn dt Temperature Differential Reference Temp Actual Reading Temperature 25 00 22 48 2 52 Temperature Compensation Factor Temperature Differential times Temperature Coefficient 2 52 x 0 000412 0 00103824 0 00103824 1 51519 1 51415176 This would be the temperature corrected refractive index at 25 C In this case if the value obtained above differs from the known value of the calibration standard by more than the accuracy tolerance for the instrument calibration of the instrument is necessary Sample prism Temperature Control Whether using ATC or not if the temperature of the sample is changing while reading the reported value will be changing as well This phenomenon was used in the previous section in a controlled way to calculate how the refractive index of a specific material changes with temperature Another example is that calibration oils typically change about 0004 refractive index units C look on the label for the specific value Controlling temperature during calibrations and when reading samples is important to accurate and reproducible readings To avoid the errors unstable sample temperature can cause the AR700 AR70 has an internal peltier which can be set for any temperature from 10C below room temperature to as high as 100C This is useful when working with samples that are not at room temperature as the instrument can be set to measure at the temperature the samples come
9. Refractive index n Sodium D line refractive index AR700 0 01 Solids 0 00001n AR70 0 1 Solids 0 0001n AR700 0 01 Solids 0 00001n AR70 0 1 Solids 0 0001n AR700 0 03 Solids 0 00005n AR70 0 1 Solids 0 0001n Typical clear samples Solids Temperature Compensated as Sucrose Temperature Compensation Range Prism Assembly Dimensions Channel 1 10 to 45 C per ICUMSA tables Channels 2 15 user definable Synthetic Sapphire mechanically sealed to Stainless Steel Well with Teflon Gasket 13 W x 16 Lx4 5 H Weight 17lbs 33cm x 40 6cm x 11 5cm 7 7kg 1 Instrument Display Digitally displays measurement values operator instructions and error messages Reichert Automatic Refractometer AR700 Main Ver 2 01 LSA Ver 1 57 CPLD Ver 0005 To calibrate press CALIBRATE or for set up press SETUP Well Temp 20 02 2 Keypad Controls All five controls are soft touch membrane keys FUNCTION down SET UP up CALIBRATE left MODE right and READ These keys other than the Read key serve a dual purpose as function keys and also facilitate moving the displayed curser highlighter when selecting the many available options through the SET UP screen 3 Sample Well Cover The hinged sample well cover is used to eliminate or minimize stray light when taking sample readings It also reduces the effects of evaporation Keeping the cover close
10. d when the instrument is not in use will prevent possible damage and contamination to the sample well and prism surface The sample well cover is made of Delrin 4 Sample Well The sample well is made of 416 grade Stainless Steel with a Sapphire Prism mounted to its center When a sample is placed on the Sapphire Prism face it is important that the sample cover the entire prism surface to attain an accurate reading Avoid any air bubbles or particulate matter from settling on the prism to reduce reading variability A Unpack instrument from carton Retain packing material for future storage protection shipping B Place the instrument indoors on a solid surface in an environment where the temperature is stable Be sure to keep instrument away from air conditioning vents or water bath exhaust drafts can affect the temperature stability C Plug the mating end of the Power Cord into the receptacle located on the back of the instrument Plug the opposite end of the Power Cord into an apropriate receptacle to supply the instrument with power Never use an adapter which will interrupt the protective grounding Supply Voltage 90 to 264V AC 47 63 HZ Input power 220W PMAX DB9 Connectors Receptacle Power Switch Air Intake D Turn the instrument on by activating the power switch located on the rear panel E Set atthe desired operating temperature in the set up screen by pressing SET UP and scrolling to Temperature Setpoint AR700
11. e the sample must be read in the Index Mode Take note of the reported value and the reading temperature The sample must then be read at a second temperature The following calculation may then be used Temperature Coefficient nD1 nD2 T1 T2 where nD1 Refractive Index of sample at temperature 1 nD2 Refractive Index of sample at temperature 2 T1 Temperature of Refractive Index reading 1 T2 Temperature of Refractive Index reading 2 The temperature coefficient is almost always a negative number in other words refractive index increases as temperature decreases and therefore decreases as temperature rises Typical temperature coefficients for fluids will be from 0 0001 to 0 0004 Once a temperature coefficient is known the temperature corrected refractive index can be calculated from an actual refractive index reading by the following formula RITC RT AT TC RI Where RITC Refractive Index Temperature Corrected RT Reference Temperature AT Actual Temperature as Read on Instrument TC Temperature Coefficient Of Sample RI Actual Refractive Index As Read On Refractometer Example Calibration Verification of an instrument using aknown fluid 1 2 Acertified calibration oil is read on an automatic refractometer as having a refractive index of 1 51519 at 22 48 C The actual value of the oil as certified by N I S T is R I 1 51416 Reference Temperature 25 C Temperature Coefficient 0 00
12. h and German and can measure refractive index of liquid solutions to a precision of 0 00005 or solids Brix to 0 03 While the instrument has many capabilities it s functionality can be expanded considerably by a simple connection to a PC Please note that a Refractometer is a high precision optical instrument It is absolutely necessary to properly maintain the instrument to obtain the best possible accuracy This includes thoroughly cleaning the prism and well after each sample routinely calibrating the instrument Indoor Use only Temperature Range 10 C 45 C Max relative humidity 80 for temperatures up 31 C decreasing linearly to 50 relative humidity at 40 C Installation category II over voltage category Pollution degree 2 Altitude up to 2 000 meters The Reichert AR7 SERIES incorporates an auto sensing switching AC power supply Never use an adapter which will interrupt the protective grounding Caution Supply Voltage Fuses Illumination Range Channel 1 Readability Precision Accuracy Risk of electrical shock exists if the cover of the AR7 SERIES is removed Hazardous voltages will be exposed which can cause injury or death Refer servicing to a qualified service technician 90 to 264V AC 47 63 HZ Input power 220W PMAX 3 15A 250VAC Quick Acting Long Life LED with 589nm bandpass filter 0 to 100 Dissolved Solids Sucrose per ICUMSA 1 32000 to 1 60000 n
13. he READ or CALIBRATE keys are pressed This parameter is set in 15 second intervals up to 4 minutes A count down timer is displayed after pressing the READ or CALIBRATE keys when this option is used CALIBRATION HISTORY Pressing the UP key will display the previous 512 calibration records Some parameters extend beyond the limits of the display to view these press the Up Dwn Lft Rt keys until the desired parameter or record is visible INDEX READABILITY Pressing the UP or DOWN key will change the index reported value from 0 00001 to 0 0001 RESTORE DEFAULTS Pressing the UP key will activate another screen with instructions to press FUNCTION to restore defaults or press SET UP to cancel This allows the user to restore all factory defaults It will not erase custom channels or calibration history More Options Press the SET UP key to advance to further Set Up options BAUD RATE Default 115200 bps Baud rate options for the RS232 serial ports are 57600 38400 19200 9600 4800 and 2400bps DATE Format is Month Day Year Use the Up Dwn Lft Rt keys to navigate and change the date TIME Can be set to standard 12 hour am pm or 24 hour military time Use the Up Dwn Lft Rt keys to navigate and change the time PASSWORD Default OFF Password protection can be used to secure options once they are set Turning this option ON and pre
14. into the lab at allowing for temperature equilibrium to be established quickly 15 Press the SET UP key to access the AR7 SERIES Setup screen Use the RIGHT LEFT keys to advance through the function choices Use the UP DOWN keys to change the default settings AR700 Setup AR700 Setup Calibration Auto Temperature Channel Interval Delay Calibration History Routine Equilibration Display Temperature Contrast Advanced Exit Index Restore More Setpoint Options Readability Defaults Options 0 00001 L R to choose option L R to choose option U D to change setting U D to change setting To exit press READ To exit press READ AR700 Setup Baud 115200 Jan 6 2003 Password Language Primary Options English L R to choose option U D to change setting To exit press READ A CALIBRATION ROUTINE Default Set Pt which is a calibration using distilled water only Set and Span uses water and up to 4 user defined standards to more accurately target a refractive index range where most readings will be made B AUTO TEMPERATURE EQUILIBRATION Default OFF Auto equilibration will automatically detect when the sample and prism have reached thermal equilibrium When this feature is activated a sample is applied and READ has been pressed the instrument will monitor the temperature until it stabilizes The instrument will then automatically take the reading During the monitoring period a graph will be
15. ion Auto Temperature Channel Routine Equilibration Set amp Span Temperature Contrast Advanced Exit Setpoint Options L R to choose option U D to change setting To exit press READ Highlight Calibration Routine using Left calibrate Right mode Up read Down function keys Change the default setting to Set amp Span using the Up Down keys Press the READ key to exit this screen Press the CALIBRATE key to continue Follow the displayed instructions to clean prism and press CALIBRATE again Clean prism and press Initiating Please wait CALIBRATE again to reinitiate or any other key to cancel Well Temp 20 01 C G Follow the on screen instructions to add Distilled Water allow to temperature equilibrate and press CALIBRATE Water calibration complete 1 33299 press SET UP to continue Multipoint Calibration Water calibration complete Use MODE to select mode 1 33299 mex rc current mode Index TC Reference Temperature 20 C Add distilled water to sample well Allow to equilibrate then press CALIBRATE Well temp 20 01C Press SETUP to continue J Select the next point using the up dwn keys to highlight the desired point for example 1 51416 Press READ to accept Note that calibration must be in ascending order of refractive index value If the calibration standard has a value other than listed move the curser to the value that is inbetween two
16. o continue I Press SET UP to continue You are now in the reading screen 1 33299 mex rc 20 00c 3 28 ou 3 32 PM Well Temp Channel 1 Sucrose ICUMSA 24 99 C J Note itis recommended to clean the air intake on the back of the instrument and exhaust located on the bottom and perform the single point distilled water calibration daily outlined above 10 mmo Also known as Multi point Calibration The AR7 SERIES can be calibrated with up to 5 points using 4 standards and distilled water Performing a 2 point calibration will provide the required accuracy needed using distilled water and the supplied high refractive index certified standard The reason for the 3 4 and 5 point calibrations is to precisely calibrate the instrument to certified standards near the refractive index range where the measurements are being made It is extremely important to note that when performing the multipoint calibration that the lowest valued standard be used first proceeding upward to the highest valued standard the standards should not be applied randomly Please see the procedure below to perform the multipoint calibration Raise the sample well cover and inspect the prism and well for cleanliness Clean with soapy water methanol or Isopropyl alcohol as required followed by distilled water then dry the prism and well Press the SET UP key on the touch panel the Set Up screen will appear AR700 Setup Calibrat
17. o deteriorate the prism seal over time or when applied at elevated temperatures Do not use acetone acetone based products or other harsh solvents when cleaning the instrument s body or it s components The prism surface should be cleaned immediately after use While the sapphire prism has excellent scratch and stain resistance the use of strong acids or bases should not be measured with this instrument or used as a cleaning agents Do not use any metal or sharp objects to clean or pry loose the prism seal or residue on the sample or well The sample should be wiped off with a dry tissue followed by a thorough cleaning Warm water and soap is recommended for removing sugar residue and methanol for oil based samples followed by a distilled water rinse Clean the dust filter located on the back of the instrument on the fan To accomplish this carefully pry the inner filter retainer from the mount there is no need to remove screws from the mount Once removed lift the filter foam from where it is positioned and either blow it clean with light air pressure or wash and rinse it in water If washed in water allow the filter to completely dry before reinstalling Reverse procedure to install 21 22 The Extended Precision Calibration Oils recommended are manufactured by Cargille Labs Cedar Grove NY 07009 1289 USA Phone 201 239 6633 To our knowledge they are the only domestic USA source of extended precision standard calibration oils with si
18. raphic Representation of Detector as Seen on Display 250000 200000 150000 100000 50000 0 oo on com N x oO 92 nn Oo 2 y ma N x DO DD oO N O9 YO yv oO Yo YO Y 99 1 2 DD oo DD N NN A ron oo ra vo 19 oo x nn 22 oo rc N T Q nr Tr Tr THT TT NNN NN NON DD 99 Pixel Number of Detector Refractive Index is a physical property of a material It changes with concentration and with temperature As a result samples need to be at a stable temperature when read and the reading will be different depending on the sample temperature This phenomenon is true for Brix or any other unit of measure that a refractometer can be programmed to report in since these are calculated from refractive index The common methods of managing this are Automatic Temperature Compensation ATC and control of the sample prism temperature Automatic Temperature Compensation Automatic Temperature Compensation ATC Since most uses of refractive index is in controlling concentration it is useful to eliminate the temperature effects when looking at instrument readings One standard method is to have programmed channels that calculate the correct reading at 20C for a sample that is being read at other temperatures This allows comparing readings to formula or process specification using 20C values without doing complicated conversions Note that custom channels to do temperature compensation are specific to the material developed for and mos
19. ssing SET UP displays the Enter Password screen Use the arrow keys to enter a 3 digit numerical password Press SET UP to exit Asecond screen confirming your password will appear Press READ to accept the new password or SET UP to cancel Once the password has been entered the AR7 SERIES will prompt the user for it each time the Set Up screen is accessed Keeping a record of the password in a secure location is recommended LANGUAGE Default ENGLISH Language can be changed to Spanish French or German by pressing the UP or DOWN keys to advance through the choices Note that the custom channel names and definitions will appear as entered in the custom channel program PRIMARY OPTIONS Press the UP key to return to the initial AR7 SERIES Set Up screen EXIT Pressing the UP key returns the AR7 SERIES to the normal display 17 18 The printer output is provided for connecting an external printer through either of two DB 9 female connectors located on the instrument s back panel Communication is by a7 bit parallel bus interface with synchronous operation The AR7 SERIES includes two serial communications ports DB 9 position female connectors located on the instrument s back panel Communications settings are 8 Data Bits No Parity 1 Stop Bit Flow Control Xon Xoff Baud Rate settings described in section 10 0 M 19200 recommended Connect to computer with a 9 pin serial
20. t likely will provide poor readings if used fora different material The automatic temperature compensation modes built into 13 Automatic temperature compensation corrects readings over arange of temperatures As an example Samples taken at 10 C 40 C are corrected to a reference temperature reading of 20 C Temperature correction is necessary because refractive index varies inversely with temperature as temperature of sample rises refractive index falls and as sample temperature drops refractive index will rise The reason for this is that a sample whose temperature is changing will report changing refractive index measurements To speed getting sample temperature to stabilize the AR7 SERIES has an internal peltier which can be set to do this it is necessary to allow the sample temperature to equilibrate so that the instrument can properly read the sample The automatic temperature compensation modes built into the AR7 SERIES are based on the temperature coefficients n C of sucrose per the ICUMSA scales When readings are taken in INDEX TC refractive index temperature compensated mode the coefficients are only valid for sucrose solutions A typical temperature coefficient for an aqueous solution is 0 0002 C and for an organic solution it is typically 0 0004 C You can calculate the correct temperature coefficient for a specific solution using the following routine To calculate the temperature coefficient of a sampl
21. units of measure such as specific gravity protein g ml etc The units of measure labels are completely definable and up to 20 characters can be used To learn more about Custom channel programming request Custom Channel Generation for Automatic Refractometer Line Be sure to inform the technical service representative of the software version in your instrument which is displayed as Ver XX X when the unit is first turned on During the opening screen when the instrument is first turned on the keypad can be locked and unlocked by pressing the READ CALIBRATE then READ MODE keys in that order 19 20 MESSAGE Refractive index of sample exceeds AR7 SERIES limits CAUSE Applied sample appears to have a refractive index greater than 1 60000 MESSAGE Refractive index of sample is below AR7 SERIES limits CAUSE Applied sample appears to have a refractive index lower than 1 32000 MESSAGE Refractive index of sample exceeds channel limits CAUSE Custom channel range does not include refractive index found MESSAGE The index of your sample was higher than expected CAUSE During calibration the refractive index of an applied standard was found to be higher than either the default or user edited index by 0 005 index or more MESSAGE The index of your sample was lower than expected CAUSE During calibration the refractive index of an applied standard was found to be lower than either the default or
22. user edited index by 0 005 index or more MESSAGE The index of your sample was more variable than expected CAUSE During calibration usually the result of the prism face not being sufficiently clean Clean prism and repeat process MESSAGE The prism is not clean or the cover is open CAUSE During initialization the sample well must be clean and dry If not this message is issued MESSAGE The temperature has changed since the last calibration Perform a water calibration to maintain accuracy CAUSE Since the last calibration has been performed the user has changed the well temperature either from the front panel setup menu or through the TEMPERATURE serial port command by an amount more than the current tolerance The tolerance defaults to 3 degrees but can be changed to 0 in which case the message is not displayed or some other value using the serial command TW The Reichert AR7 SERIES is a precision optical instrument The prism surface and well should be cleaned immediately after use The interior components are protected from dust spills and other damage The stainless steel well and prism assembly is sealed with a Teflon gasket All non optical components can be cleaned with soap and water or methanol Using strong solvents such as N N Dimethylformamide or N N Nimethylacetamide cresols phenols and other tar acids should be avoided Tetrahydrofuran or some lacquer thinners and their components can als
23. values that bracket the standards value For example if the standard value is 1 52146 move curser to 1 51416 since 1 52146 is higher than 1 45755 and lower than 1 57230 Proceed to step I SPAN CALIBRATION Highlight point select with READ Repeat with additional points as needed When complete select EXIT Refractive Brix Last Index Equivalent Calibrated 1 24 1 40229 1 45755 67 72 1 51416 88 73 1 57230 N H EXIT Use U D to highlight READ to select Adjust the refractive index value which should now be displayed to the value listed on the bottle of calibration fluid by pressing SET UP Use the up dwn left right keys to adjust value When the value matches press READ to accept and continue Adjust temperature coefficient value or press READ to continue Change reference temperature or press READ to continue If the displayed value matches the value as listed on the bottle of calibration oil press CALIBRATE to continue Calibration Standard Index 1 51416 Press SETUP to edit Press CALIBRATE to continue Add the stated Calibration Standard ex 1 51416 to cover prism allow to temperature equilibrate and press CALIBRATE to continue K Calibration point complete press SET UP to continue L Calibration point complete 1 51416 mex Reference Temperature 25 C Press SETUP to continue Highlight another calibration point or EXIT and press READ to select SPAN
24. x significant figures in index The characteristics of these oils are NIST traceable Stated accuracy at 0 00005 n Temperature coefficients TC are guaranteed at their specified reference temperatures usually 20 or 25 C 5 year shelf life Catalog No 13K41330 Calibration Oil 1 51432 Catalog No 13K41340 Calibration Oil 1 40424 Catalog No 13K41350 Calibration Oil 1 45755 Catalog No 13K41360 Calibration Oil 1 48993 Catalog No 13K41370 Calibration Oil 1 57230 NOTE The values for the above calibration oils are offered as a general range and may change somewhat by the supplier of these oils due to their manufacturing process A document package accompanies each bottle stating all of the appropriate values When using any new oil for calibration those values should be confirmed Catalog No 13107100 Flow Cell with Temperature Control The flow cell allows samples to be pumped to the prism surface Typically a peristaltic pump with a flow rate of approximately 10 15 ml min is used Use of the flow cell generally increases instrument precision and eliminates errors due to an unclean prism surface Use of the flow cell also allows the user to pre temperature equilibrate extremely hot or cold samples Ports are provided for circulation of a controlled temperature fluid Catalog No 13107200 Funnel Flow Cell with Temperature Control This accessory offers the same benefits as the flow cell but eliminates the
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