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Varian 900-LC Series Liquid Chromatograph

1. N z Standard Ethernet Cable SS Q ry 8 Galaxie Standard Ethernet Cables 900 LC 900 LC 900 LC 900 LC Figure 3 Multiple 900 LC Series HPLC Systems in standalone configuration Up to four systems can be controlled by one Galaxie Acquisition Server For a client server system there will need to be a PC with the Galaxie Acquisition Server running for every four HPLC systems Dedicated Subnet for 900 LC s Standard Ethernet Cable O Standard Ethernet Cable S Q i D Galaxie Acquisition Server and Client PC Standard Ethernet Cables Galaxie Server 900 LC 900 LC 900 LC 900 LC Figure 4 Networked multiple 900 LC Series HPLC System configuration option 1 Publication no 8510243600 Issue 1 February 2008 Page 21 of 54 Varian 900 LC Series Liquid Chromatograph Standard Ethernet Cable Dedicated Subnet for 900 LC s Standard Ethernet Cable Galaxie Client PC Q i Galaxie Server and Galaxie Acquisition Server Standard Ethernet Cables 900 LC 900 LC 900 LC 900 LC Figure 5 Networked multiple 900 LC Series HPLC System configuration option 2 ec Note Page 22 of 54 You or your network administrator must provide any hardware adapters cables and configuration and setup work required to convert from the company network wiring type to 10 100 BaseT wiring This hardware must comply with the IEEE 802 3
2. Publication no 8510243600 Issue 1 February 2008 Page 43 of 54 Varian 900 LC Series Liquid Chromatograph 3 5 Page 44 of 54 e select normalization for the PDA detector e set the option to turn the lamps on as the detector s power up e choose synchronize with other modules send a contact closure to external devices e choose which leak detector action will be performed when a leak is detected e choose solvents to be used in the valve module section if you have a Scale Up Module e with semi prep and prep systems you can choose the pump head sizes sample delivery pump head size and pressure units e in the fraction collector section you must choose your rack type tube volume and probe depth e save your settings Creating a Method Note Note Note To create a new method 1 Click File gt New gt Method in the upper left corner of the Galaxie software 2 Choose the Project Name and System name from the drop down menus and then click Next 3 Enter the Method Name and Description and then click OK Galaxie will open to the main method screen on the Integration Events tab 4 in the lower left corner click on control The Galaxie software will show the Device Select window You should see a list of your system components just to the left of the Device Select window 5 Choose the devices needed for your run by selecting or deselecting the options in the Device Select window 6 On ea
3. Shipping dimensions Table 6 900 LC Series Liquid Chromatograph and options dimensions Warning The HPLC system weighs up to 130 kg 287 Ib To avoid injury to personnel or damage to equipment always use a forklift or other suitable lifting device when moving the instrument 1 4 Personal Computer Requirements The recommended and minimum PC specifications are listed in the following table They can also be found on Varian s Web site at www varianinc com The minimum configuration represents the absolute minimum that is required to run the Galaxie software This PC configuration may be out of manufacture but you may Publication no 8510243600 Issue 1 February 2008 Page 17 of 54 Varian 900 LC Series Liquid Chromatograph want to use a PC you already have The recommended configuration should be followed when buying a new PC Minimum Recommended Pentium 4 processor 2 8 GHz or Core 2 Duo 2 13 GHz or higher higher 1 GB of RAM 2 GB of RAM 160 GB hard drive 2 x 200 GB hard drives 16 speed CD ROM or DVD RW 16 speed CD ROM or DVD RW Super VGA monitor with high color 16 Super VGA monitor with high color 16 bit display 1024 x 768 resolution bit display 1024 x 768 resolution minimum 17 monitor minimum 17 monitor 16 bit sound card 16 bit sound card Windows compatible keyboard Windows compatible keyboard Microsoft or compatible mouse Microsoft or compatible mo
4. Click Program a method from start to finish for a step by step tutorial on how to program a method 3 6 Starting an Acquisition There are two ways to start an acquisition You can have multiple injections by creating a sequence or single injections using Quick Start Publication no 8510243600 Issue 1 February 2008 Page 45 of 54 Varian 900 LC Series Liquid Chromatograph 3 6 1 3 6 2 Page 46 of 54 Sequence 1 From the File menu click New gt New Sequence 2 Choose the Project name and System name to be associated with this sequence and then click Next 3 Enter the number of lines number of samples in the sequence and then click Next 4 Enter the new sequence name and a description if needed and then click OK Note A window appears with the number of lines you chose in step 3 You can auto fill the columns by entering or choosing a value in the first line and then right clicking the heading of that column Choose Fill Block and then follow the prompts Alternatively you can manually fill in each field to customize your sequence 1 2 3 Enter in all required fields Click File gt Save gt Save Sequence On the Sequence page click the green arrow button to start the sequence To view your runs click the Systems tab on the bottom left of the Galaxie software Note For further information about starting an acquisition and interpreting the results refer to the Galaxie Chromatography Data System
5. Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Nitrogen Gas Purity gt 98 filtered to 0 2 um Permissible pressure 60 to 100 psi range 4 6 7 bar Maximum pressure 100 psi 6 7 bar regulated Required flow rates Up to 3 25 L min at 60 psi at 25 C Table 5 Gas requirements Helium may also be required to if you are using a helium sparging system to degas solvents Consult the documentation that came with your helium sparging system for gas requirements 1 3 3 1 Storage Cylinder Instructions Cylinders containing gas under pressure should be firmly secured to a rigid structure and the storage area must be adequately ventilated Never locate gas cylinders near a source of ignition or in a position that is subject to direct heat Gas storage cylinders often incorporate a pressure release device which will discharge the gas at a predetermined temperature usually around 52 C 125 F If gases are to be plumbed from a remote storage area to the instrument site ensure that the local outlets are fitted with stop valves pressure gauges and suitable regulators which are easily accessible to the instrument operator The gas outlets must be provided within 1 5 meters 5 feet of the instrument 1 3 4 Waste Container The Varian 900 LC Series Liquid Chromatograph needs a drain vessel for disposal of fluids and vapors from the system Suitable tubing is supplied with the HPLC system fo
6. designed so that when used properly you have an accurate fast flexible and safe HPLC system Information about safety practices appears throughout the documentation both hard copy and online provided with your instrument Before using the instrument you must thoroughly read these safety practices Observe all relevant safety practices at all times This instrument is designed for chromatographic analysis of appropriately prepared samples It must be operated using appropriate solvents and within specified maximum ranges for pressure flows and temperatures as described in this manual If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired It is your responsibility to inform Varian representatives if the instrument has been used for the analysis of hazardous biological radioactive or toxic samples prior to any instrument service being performed or when an instrument is being returned to the service center for repair or is being recycled at end of life General Operation of a Varian 900 LC Series Liquid Chromatograph involves the use of pressurized liquid ultraviolet radiation and hazardous materials including corrosive fluids and flammable liquids Careless improper or unskilled use of this liquid chromatograph can cause death or serious injury to personnel and or severe damage to equipment and property The 900 LC Series Liquid Chromatograph incor
7. 2 4 Hardware Installation Your HPLC system will be ready to operate after the Varian representative has installed it However over time you may need to replace some hardware components such as detector lamps autosampler needles etc or may need to install a new device to Publication no 8510243600 Issue 1 February 2008 Page 33 of 54 Varian 900 LC Series Liquid Chromatograph 2 5 2 6 Page 34 of 54 your HPLC system For instructions on how to do this please refer to the online help Specifically 1 Click the Windows Start button and choose Programs gt Galaxie gt Help gt Varian 900 LC Series HPLC Help 2 When the Online Help appears click the How to gt Installing Components link to access step by step instructions on how to remove and install the various instrument components For information on your instrument connections including connecting the network and communication cables please refer to the online help 1 Click the Windows Start button and choose Programs gt Galaxie gt Help gt Varian 900 LC Series HPLC Help 2 When the Online Help appears click About Your Instrument link to access information about the front side and back connections and components of your HPLC system Installation System Test Your Varian representative will perform a system test during the installation procedure You can find the results of the system test in the Log Book The Log Book is a list of all a
8. Configuration Manager then select the options menu and then select the option Licenses The installer begins to install the driver files onto the PC 6 Click Yes to install the Flash Player If you click No you will not get the full functionality of the 900 LC Series HPLC driver however Flash is not required to run the 900 LC 7 Click Close on the Flash Player 9 Installer Setup Completed window to continue the installation 8 Click Next on the Welcome to Microsoft NET Framework 2 0 Setup window 9 Read and then accept the terms of the License Agreement and then click Install on the End User License Agreement window 10 Click Finish to complete the Microsoft NET Framework 2 0 installation This process can take several minutes 11 Click Yes to install Adobe Acrobat Reader If you click No installing Adobe Acrobat Reader will be skipped However you may not be able to view any accompanying PDF report files or help files If you click Yes and the Repair Modify window appears Adobe Acrobat Reader is already installed on your PC Click Cancel to stop the Adobe Acrobat Reader installation or click Repair to re install 12 Click Next on the Destination Folder window 13 Click Install on the Ready to Install the Program window 14 Click Finish to complete the Adobe Acrobat Reader installation Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 2 2 6 I
9. Online Help Quick Start To start a Quick Start run 1 2 3 Click Acquisition on the top menu bar Choose Quick Start Select the Project name System name and Method name and then click OK Click on the button with the three dots next to the File prefix field to open up variable options for your run name Double click the variables to be used in your run name or type in a name for your run and then click OK Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 5 Set the identifier number if something other than 1 is required to name the run 6 In the Acquisition Parameters section enter in the Vial and Rack number if an autosampler is to be used the Acquisition length and Injection Volume 7 Click the Start button 8 To view your run click the Systems tab on the bottom left of the Galaxie software gt Note For further information about running samples and interpreting the results refer to the Galaxie Chromatography Data System Online Help 3 7 Printing a report To print a report 1 Click Report Style in the lower left corner of the Galaxie software gt Note When printing a report you can simply select Print from the File menu However if you do so a report will be generated based upon the last used Report Settings You will not be able to select the data to be included in the printout 2 Choose a report style or edit a style c No
10. be tracked e The Log Book lists all actions and counter information and can be saved in a PDF file e Service tests which can test the performance of devices in the HPLC system 2 8 1 How to Access IMS Access the Instrument Maintenance Software from the link on the right side of the Status Overview window in the Galaxie software The Instrument Maintenance software can be run with the Galaxie version 1 9 SP1 or greater When the logon window appears enter a User identification and choose the group and project in which the system to be maintained is defined in Galaxie Type the corresponding password The password is case sensitive abc ABC To be able to log into the Instrument Maintenance Software allow to run instrument maintenance software must be checked in the Acquisition system properties part of the user profile in the Galaxie Configuration Manager software If the user does not have the right to run maintenance software an error message is displayed Publication no 8510243600 Issue 1 February 2008 Page 35 of 54 Varian 900 LC Series Liquid Chromatograph 2 8 2 Page 36 of 54 To enable IMS access About IMS 1 Open and log in to the Galaxie Configuration Manager software Click User profiles Right click on the user profile to be edited and choose Edit Click Next Click the sign next to Acquisitions System properties to expand the list Select Allow to run instrument maintenance s
11. grounding earthing is essential to avoid a potentially serious electric shock hazard Consult the manuals or product labels supplied with your PC for the relevant grounding requirements Replace blown fuses with fuses of the size and rating shown on the fuse panel or in the manual Replace faulty or frayed power cords immediately with the same type and rating Make sure that voltage sources and line voltage match the value for which the instrument is wired Avoid using power supplies from a source that may be subject to electrical or RF interface from other services for example large electrical motors elevators and welders Compressed Gas Cylinders Unless your system is equipped with a helium sparging unit or an ELS detector there are no gas requirements for the 900 LC Series HPLC systems For gas requirements on these devices consult the Operation Manual or other documentation enclosed with the particular device Compressed gas cylinders contain highly pressurized gas If storage conditions are outside of the recommended suppliers safety codes the cylinders can explode or rapidly release gas into the environment This may result in injury or death Store and handle compressed gases carefully and in strict adherence to safety codes Secure cylinders to an immovable structure or wall Store and move cylinders in an upright vertical position Before transport remove regulators and install cylinder cap Store cylinders in
12. inside the sub directory the path is displayed at the top of the window Page 48 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 4 Maintenance This chapter includes information on how to access the Varian 900 LC Series HPLC system maintenance requirements that may be carried out by an operator Any maintenance procedures not specifically mentioned should be carried out only by Varian trained Varian qualified or Varian authorized representatives Warning The UV Vis PDA and Fluorescence detector lamps are intense light sources Direct viewing of the light source will cause eye damage Operators and other unauthorized personnel must NEVER remove the main covers Warning This instrument contains electrical circuits devices and components operating at dangerous voltages Contact with these circuits devices and components can result in death cause serious injury or painful electrical shock Warning Allow hot parts to cool before proceeding with any maintenance procedure Publication no 8510243600 Issue 1 February 2008 Page 49 of 54 Varian 900 LC Series Liquid Chromatograph Warning There is a danger of ignition when using flammable chemicals such as organic solvent Aqueous solvents or organic solvents having an ignition point above 70 C can be used Do not use organic solvents having an ignition point below 70 C Be careful about igni
13. standard Choose the appropriate set up for your laboratory Use standard Ethernet cables Your 900 LC Series HPLC system comes with two Ethernet cables and a network switch You will need to provide additional cables based on your HPLC system and PC configuration To connect the Ethernet cables You must connect the 900 LC Series HPLC system to the PC running the Galaxie Acquisition server via the network switch provided This will ensure uninterrupted connection between these two devices 1 Connect an Ethernet cable from the Network PC port on the side of the 900 LC Series HPLC system to the provided Ethernet switch Consult the instructions provided with the switch for more information 2 Network PC Scale Up Module P Figure 6 Side of 900 LC Series HPLC This image has been Analog in Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph rotated to show horizontal The connections are vertical on the side of the HPLC system 2 Connect an Ethernet cable from the Ethernet switch to the Ethernet port on the PC s and or company network 3 Connect the switch to your company network if required 2 2 Installing the Galaxie Chromatography Data System Software Varian recommends that you purchase a PC as part of the Varian 900 LC Series Liquid Chromatograph package The PC included in the package will come with the appropriate Windows operating system as loaded by
14. the PC supplier For instructions on installing the Windows operating system please refer to the appropriate manuals supplied with the software It is your responsibility to ensure that the operating system software has been installed and is functional if you are supplying the PC or the operating system c Note Itis important to set up user profiles with permissions to access the Galaxie software on each PC used with the 900 LC Series HPLC and a user profile for the administrator to have access to the Galaxie Configuration Manager The 900 LC Series HPLC system requires Galaxie software version 1 9 SP1 or greater a serial number for either the Galaxie Varian HPLC Drivers or the Varian 900 LC Series Driver and the 900 LC Series Driver software If you do not have Galaxie Software version 1 9 SP1 or greater you must upgrade to run the 900 LC Series driver gt Note Varian will not assume responsibility for loss of data Your Varian representative will connect the PC to the HPLC system and any factory approved accessories purchased at installation Initial instrument software installation is also included as part of the system installation Although the Varian trained Varian qualified or Varian authorized representative will install the Galaxie software for you during the installation process you may need to install the software yourself at some later stage for example if you change the PC 2 2 1 Software Components Requir
15. this information 1 Select the Windows Start button and choose Programs gt Galaxie gt Help gt Varian 900 LC Series HPLC Help When the Online Help appears click the How to link to view the available step by step instructions Below is a quick guide to getting the instrument set up and running samples 3 1 Analysis Checklist You need to complete the following steps in turn to measure a sample s You will find details on each step in this chapter Turn on the instrument and software Prepare the instrument for use Configure the Device Settings and System Parameters Create a method Run samples View or Print a report 3 2 Turning on the Instrument and Software Before starting the system carefully read the Safety practices and hazards section at the front of this manual and ensure that the laboratory is set up according to the details specified in the Pre installation manual To turn on the instrument and software 1 2 Switch on the computer monitor and printer Turn on the HPLC system Publication no 8510243600 Issue 1 February 2008 Page 39 of 54 Varian 900 LC Series Liquid Chromatograph 3 2 1 3 3 Page 40 of 54 3 To start the Galaxie software click the Windows Start button and then choose Programs gt Galaxie gt Galaxie 4 Enter in your login details and click OK Connecting to a System Note Depending on the configuration you purchased you may have up t
16. 00 LC Series Liquid Chromatograph CE Declaration of Conformity We hereby declare that the equipment listed below complies with the requirements of The Low Voltage Directive 73 23 EEC 93 68 EEC The EMC Directive 89 336 EEC 92 31 EEC and 93 68 EEC Applicable Standards BS EN 61010 1 2001 EN 61326 2003 EN 55011 1998 EN 61000 3 11 2000 EN 61000 4 2 1995 EN 61000 4 3 1995 EN 61000 4 4 1995 EN 61000 4 5 1995 EN 61000 4 6 1996 EN 61000 4 11 1994 Equipment Model Number Varian 920 LC 940 LC HPLC Systems Authorized Representative in the EU Name G A Wassink Company Name Varian BV y Address Herculesweg 8 4330 EA Middelburg Signed The Netherlands Position Quality Manager Telephone 31 0 118 671 000 Date 1 December 2007 Facsimile 31 0 118 633 118 Manufacturer Name Philip Binns Company Name Varian Australia Pty Ltd 2 Address 679 Springvale Road VAA Mulgrave VIC 3170 Signed 7 Australia Position Director of Operations Telephone 61 0 3 9560 7133 Date 1 December 2007 Facsimile 61 0 3 9560 7950 Publication number 8510244500 December 2007 VA R l A N Publication no 8510243600 Issue 1 February 2008 ll Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank iv Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Contents Safety Practices and Hazards General Electrical Hazards Compressed Gas Cylinders Storage Cylinder Instru
17. 1 3 1 Page 12 of 54 signify the pushbuttons appearing throughout the software e g click OK e ALL CAPITALS indicate keyboard and mouse commands e g press the F2 key and text you must type in from the keyboard e g type SETUP at the prompt Notes and Hot Tips A Note is used to give advice or information A Note is denoted by the following symbol ee A Hot Tip is used to give practical hints to help you achieve the best possible performance from your instrument A Hot Tip is denoted by the following symbol vetei Specifications The instrument is suitable for indoor use only and is classified suitable under the following categories EN 61010 1 Installation category Il Pollution degree 2 Safety class 1 Environmental Conditions Note Condition Altitude Temp Humidity RH C non condensing Non operating 0 3 050 m 5 45 20 80 Transport 0 10 000 ft Non operating amp Sea level 40 90 95 meeting dielectric strength tests Operating within 0 2 000 m 10 35 8 80 specifications 0 6 560 ft Table 1 Environmental conditions For optimum analytical performance it is recommended that the ambient temperature of the laboratory be between 20 and 25 C and be held constant to within 2 C throughout the entire working day Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 1 3 2 Electrical Power Supplies All power supplies should be single p
18. 1 of 54 Varian 900 LC Series Liquid Chromatograph 2 2 9 2 2 10 Page 32 of 54 10 If your changes are not showing in Galaxie Configuration Manager press F5 on your keyboard or View and then Refresh to refresh the window 11 Click Interface configuration on the bottom left side of the window 12 Highlight the first line with the system name to be deleted and then click Remove on the upper right side of the window 13 Repeat the previous step for all entries prefaced with the system name to be deleted 14 Click Apply 15 Exit Galaxie Configuration Manager Change the 900 LC Series HPLC System Name or IP Address You cannot change the name or IP address of an HPLC system You must delete the system first and then re create it using the SetupWizard exe To change the name or IP address of a system 1 Delete the HPLC system See section 2 2 8 for instructions 2 Re create the HPLC system See section 2 2 7 for instructions Update Galaxie to Version 1 9 SP1 Note The 900 LC Series HPLC Driver requires Galaxie version 1 9 SP1 to run The ordering information for the CD is part number 03 950000 98 Galaxie Software Service Pack 1 SP1 CD This CD is only available for Galaxie software version 1 9 Contact your local Varian representative to upgrade Galaxie version 1 8 to 1 9 SP1 This CD will not update Galaxie version 1 8 to 1 9 SP1 To update to version 1 9 SP1 1 Insert the CD into your CD ROM dr
19. 7 Configuring the 900 LC Series HPLC System s 29 2 2 8 Deleting a System 31 2 2 9 Change the 900 LC Series HPLC System Name or IP Address 32 2 2 10Update Galaxie to Version 1 9 SP1 32 About the Varian 900 LC Series HPLC Online Help 33 Hardware Installation 33 Installation System Test 34 Customer Training 34 Using the Varian 900 LC Series HPLC Online Help 35 Instrument Maintenance Software IMS 35 2 8 1 How to Access IMS 35 2 8 2 About IMS 36 Operation 39 Analysis Checklist 39 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 3 2 Turning on the Instrument and Software 39 3 2 1 Connecting to a System 40 3 3 Preparing the System for Use 40 3 4 Configuring the Device Settings and System Parameters 41 3 4 1 Flows 42 3 4 2 Detectors 42 3 4 3 Column 42 3 4 4 Sample Introduction 43 3 4 5 System Parameters 43 3 5 Creating a Method 44 3 6 Starting an Acquisition 45 3 6 1 Sequence 46 3 6 2 Quick Start 46 3 7 Printing a report 47 3 8 Saving Your Files 47 4 Maintenance 49 4 1 Routine Maintenance 50 4 2 Cleaning 51 4 3 Spare Parts 51 5 Troubleshooting 53 Publication no 8510243600 Issue 1 February 2008 vii Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank viii Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Safety Practices and Hazards Your Varian instrument and accessories have been carefully
20. Galaxie Workstation Stand alone Installation Information When using a stand alone configuration the software for the main server acquisition server and client are installed on one PC This PC can then be used to control up to four 900 LC Series HPLC systems 2 2 4 Galaxie Installation Procedure The 900 LC Series Driver installation has several steps 1 Install the Galaxie core software See the Galaxie Chromatography Data System Installation manual for instructions to install the software and configure user permissions 2 Install other HPLC or GC drivers as required This step is part of the Galaxie core installation 3 Install the 900 LC Series HPLC driver The installation software for the 900 LC Series HPLC driver is a separate CD to the Galaxie software installer 4 Install the 900 LC Series HPLC Online Help The 900 LC Series HPLC Online Help CD is a separate CD to the driver CD but will be requested by the driver CD during installation 5 Configure the 900 LC Series HPLC system s on the acquisition server Publication no 8510243600 Issue 1 February 2008 Page 25 of 54 Varian 900 LC Series Liquid Chromatograph 2 2 4 1 Page 26 of 54 Note Note Note Steps 1 2 are performed using the Galaxie CD Steps 3 5 are performed using the 900 LC Series driver setup wizard CD and the Online Help CD Before installation you must have the HPLC system set up and turned on Set up includes having the networ
21. Galaxie software installation For information on how to delete a system please see section 2 2 8 Deleting a System page 31 gt Note Before installation you must have the HPLC system set up and turned on Set up includes having the network cabling and hardware plugged in To install the 900 LC Series HPLC Driver 1 Insert the 900 LC Series Galaxie Driver CD into your CD ROM drive The software will automatically start 2 Click Next on the Welcome to Install Shield Wizard for Varian Series 900 LC Drivers window 3 Click Yes to accept to the License agreement Clicking no will terminate the program If you have turned off the auto start capability of your CD ROM drive you will need to run the setup exe file on the CD ROM Publication no 8510243600 Issue 1 February 2008 Page 27 of 54 Varian 900 LC Series Liquid Chromatograph Page 28 of 54 Note Note 4 Enter in your name the company name and the serial number of the 900 LC Series HPLC Driver CD p n 8510244200 or the serial number from the Galaxie Control Software for Varian HPLC Systems CD p n 0395000491 The serial number is provided on your Varian HPLC driver s serial number card or Galaxie software CD and is different from the Galaxie serial number 5 For installations of the new Varian 900 LC Driver with an existing Galaxie Varian HPLC driver only If you need to look up existing serial number in the Galaxie software first login to the Galaxie
22. Issue 1 February 2008 Page 7 of 54 Varian 900 LC Series Liquid Chromatograph Warning Symbols The following is a list of symbols that appear in conjunction with warnings in this manual or on the 900 LC Series Liquid Chromatograph The hazard they describe is also shown A triangular symbol indicates a warning The meanings of the symbols that may appear alongside warnings in the documentation or on the instrument itself are as follows gt gt gt Corrosive liquid Electrical shock Explosion hazard gt gt gt Eye hazard Fire hazard Heavy weight danger to feet gt gt gt Heavy weight Hot surface Moving parts danger to hands gt Radiation source The following symbol may be used on warning labels attached to the instrument When you see this symbol refer to the relevant operation or service manual for the correct procedure referred to by that warning label Page 8 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph The following symbols appear on the instrument for your information Mains power on off button Press the power button in and release it to turn it on Press the power button in for 5 seconds and then release it 9 to turn the HPLC system off There may be a short delay between releasing the power button and instrument shut down Fuse Single phase alternating current Direct current When attached to the r
23. Varian 900 LC Series Liquid Chromatograph Getting Started Manual Installation Category II Pollution Degree 2 Safety Class 1 EN 61010 1 NOTICE This document contains references to Varian Please note that Varian Inc is now part of Agilent 8510243600 Technologies For more information go to February 2008 www agilent com chem Issue 1 ott Agilent Technologies Varian 900 LC Series Liquid Chromatograph Varian Inc Serving Industries Worldwide Biosciences Pharmaceuticals Clinical Research and Forensics Food and Agriculture Chemical Analysis Environmental Fuels and Energy Material Sciences Sales and Service Contact Details North America 800 926 3000 925 939 2400 Europe The Netherlands 31 118 67 1000 Asia Pacific Australia 613 9560 7133 Latin America Brazil 55 11 3238 0400 Varian Inc Web Site www varianinc com Varian Inc is the owner of copyright on this document and any associated software Under law the written permission of Varian Inc must be obtained before either the documentation or the software is copied reproduced translated or converted to electronic or other machine readable form in whole or in part First published in February 2008 Inspiring Excellence Varian and the Varian logo are trademarks or registered trademarks of Varian Inc in the US and other countries 2005 2008 Varian Inc Publication no 8510243600 Issue 1 February 2008 Varian 9
24. a well ventilated area away from heat direct sunshine freezing temperatures and ignition sources Mark cylinders clearly so there is no doubt as to their contents Use only approved regulators and connections Publication no 8510243600 Issue 1 February 2008 Page 3 of 54 Varian 900 LC Series Liquid Chromatograph Page 4 of 54 e Use only connector tubing that is chromatographically clean Varian part number 0391832600 and has a pressure rating significantly greater than the highest outlet pressure from the regulator Storage Cylinder Instructions Cylinders containing gas under pressure should be firmly secured to a rigid structure and the storage area must be adequately ventilated Never locate gas cylinders near a source of ignition or in a position that is subject to direct heat Gas storage cylinders often incorporate a pressure release device which will discharge the gas at a predetermined temperature usually around 52 C 125 F If gases are to be plumbed from a remote storage area to the instrument site ensure that the local outlets are fitted with stop valves pressure gauges and suitable regulators which are easily accessible to the instrument operator The gas outlets must be provided within 1 5 metres 5 feet of the instrument High Pressure Hazards If a line ruptures a relief device opens or a valve opens accidentally under pressure potentially hazardous high liquid pressures can be generated by th
25. al number will need to be entered twice both when installing Varian HPLC Drivers and when installing the 900 LC Series driver You will only need to purchase the one serial number For subsequent installs the serial number is recognized Galaxie CDS Client Server Installation Information There are three different components in a Galaxie CDS client server installation The three components can be installed on separate PCs or combined on the one PC See the Galaxie Installation manual for more information e Main server where data and methods are stored Configure only one main server when installing the Galaxie CDS software Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph e Acquisition server communicates with the hardware of the 900 LC Series HPLC system Up to four 900 LC systems can be run by one acquisition server There can be several acquisition servers in a Galaxie CDS installation e Client methods are created data viewed and reprocessed There can be several clients in a Galaxie CDS installation gt Note The 900 LC Series driver must be installed on all PCs in the Galaxie network regardless of whether it is a main server acquisition server or client PC System configuration should only be done on the acquisition server when using the 900 LC Series driver setup wizard The online help must be installed on every PC where it may need to be viewed 2 2 3
26. ary 2008 Page 5 of 54 Varian 900 LC Series Liquid Chromatograph Page 6 of 54 e Leakage of solvent inside the instrument When using flammable chemicals be careful about possible ignition due to static electricity To prevent the build up of static electricity use a conductive container for waste Use only approved regulator and hose connectors refer to the supplier s instructions Keep solvents cool and properly labeled Ensure that you have the correct solvent before connecting it to the instrument Inflammation or Injury Due to Toxic Corrosive or Stimulative Solvent When using a toxic corrosive or stimulative solvent be careful not to contact solvent to prevent physical inflammation or injury For details of the properties of each solvent and how to handle it refer to the relevant Material Safety Data Sheets MSDS Be sure to handle each solvent properly Wear proper personal protective clothes e g safety goggles so that a solvent will not come into direct contact with the skin Ventilate the laboratory room adequately to prevent accidental inhalation of harmful solvent vapor Other Precautions Use of the 900 LC Series Liquid Chromatograph and accessories may involve materials solvents and solutions which are flammable corrosive toxic or otherwise hazardous Careless improper or unskilled use of such materials solvents and solutions can create explosion hazards fire hazards toxicity and other hazard
27. ayed on screen autosampler rack size fraction collector rack sizes leak detection actions pump head size sample loop size and enabling the cooling on the autosampler trays For additional information on your system components please see the 900 LC Series HPLC Online Help Flows In Flows you can manually set and turn on your flow rate and solvent composition purge the pumps set the bottle management parameters select the solvents for the Scale Up Module valves if present in your system Detectors Depending on the detector you purchased in the detector Device Settings you can Column choose wavelengths autozero the detectors turn your detector lamp s on choose the data collection parameters set the detector temperature conditions Depending on the column options you purchased you can set the column heater temperature turn the heater on choose the scale up module column choose between two columns if a column switching valve is present view the column properties of your column if you have downloaded your method to the HPLC system Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 3 4 4 Sample Introduction Sample introduction can occur by using an autosampler or manual injector if you are running an analytical or analytical to semi preparative HPLC If you are running a preparative scale HPLC system samples are introduced using a manual injector or b
28. ch device window set your parameters Your System Parameters will automatically appear in your method All other settings will need to be checked 7 Click acquisition in the lower left corner of the Galaxie software Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 10 Click on the button with the three dots next to the File prefix field to open up variable options for your run name Double click the variables to be used in your run name or type in a name for your run and then click OK Set the identifier number if something other than 1 is required to name the run In the Acquisition Parameters section enter in the Vial and Rack number if an autosampler is to be used the Acquisition length and Injection Volume c Note For information about the additional fields in the Acquisition window please see your Galaxie Chromatography Data System software Online Help 1 Click File gt Save gt Save Method The method will now be saved in the default data folder For more information on creating a method please see the 900 LC Series HPLC Help 1 N Oo a FF a DN oa Click the Windows Start button Click Programs Click Galaxie Click Help Click Varian 900 LC Series HPLC Help Click the plus sign next to Software Click Galaxie software tutorial for a basic overview of the main Galaxie windows Alternatively click the plus sign next to How To
29. ck Help Click Varian 900 LC Series HPLC Help Click the Maintenance link Click Checklist Any spills in the sample compartment or on the HPLC system should be wiped up immediately The exterior surfaces of the instrument should be kept clean All cleaning should be done with a soft cloth If necessary this cloth can be dampened with water or a mild detergent Do not use organic solvents or abrasive cleaning agents 4 3 Spare Parts For spare parts and consumables ordering information refer to the Varian Inc Web site www varianinc com Publication no 8510243600 Issue 1 February 2008 Page 51 of 54 Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank Page 52 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 5 Troubleshooting For troubleshooting help please refer to the Varian 900 LC Series HPLC online help To access the online help troubleshooting section 1 Click the Windows Start button 2 Click Programs 3 Click Galaxie 4 Click Help 5 Click Varian 900 LC Series HPLC Help 6 Click the Troubleshooting link for general HPLC system troubleshooting or click the plus sign next to Troubleshooting to access the device troubleshooting information Publication no 8510243600 Issue 1 February 2008 Page 53 of 54 Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank Page 54 o
30. co chemical properties of the components of the mobile phase Only use solvents compatible with the HPLC system tubing and fittings as certain solvents may cause weakening and leaks with possible bursting Employ static measuring and static discharge devices to safeguard against the buildup of static electricity All solvents can create a hazard if they leak into the atmosphere Even small leaks in solvent supply systems can be dangerous Any leak except that of air can result in an oxygen deficient atmosphere which can cause asphyxiation The area in which solvents are stored and the area surrounding the instrument must be adequately ventilated to prevent such accumulations Ignition of Flammable Chemicals This instrument is not explosion proof In unattended operation do not use organic solvents having an ignition point below 70 C If a flammable chemical such as organic solvent leaks from the flow path of the instrument and its vapor concentration reaches the explosion limit it could cause spontaneous combustion with dangerously explosive results Beware of ignition hazard when using flammable chemicals such as organic solvents Do not bring a heat or flame source near the instrument Ventilate the laboratory room where the instrument is used Always check the following conditions If an abnormality is found stop operation immediately e Leakage of solvent or waste solution Publication no 8510243600 Issue 1 Febru
31. ctions High Pressure Hazards Ultraviolet Radiation Solvent Hazards Ignition of Flammable Chemicals aoon Aa B amp B BR WORD B a Inflammation or Injury Due to Toxic Corrosive or Stimulative Solvent Other Precautions Warning and Caution Messages Warning Symbols Color Coding US FCC Advisory Statement Spurious and Harmonic Radiation Compliance CE Compliance 1 Introduction 1 1 Pre installation Requirements 1 2 User Documentation 1 2 1 Conventions 1 2 2 Notes and Hot Tips 1 3 Specifications 1 3 1 Environmental Conditions 1 3 2 Electrical Power Supplies 1 3 3 Gas Supplies Publication no 8510243600 Issue 1 February 2008 oO ON OD OD 10 11 11 11 11 12 12 12 13 14 Varian 900 LC Series Liquid Chromatograph vi 1 4 2 1 2 2 2 3 2 4 2 5 2 6 2 7 2 8 1 3 4 Waste Container 15 1 3 5 Laboratory Conditions 16 1 3 6 Instrument Cooling Air Supply 16 1 3 7 Weights and Dimensions 16 Personal Computer Requirements 17 Installation 19 900 LC Series HPLC System to PC Communications 19 Installing the Galaxie Chromatography Data System Software 23 2 2 1 Software Components Requiring Installation and Serial Numbers Used During Installation 23 2 2 2 Galaxie CDS Client Server Installation Information 24 2 2 3 Galaxie Workstation Stand alone Installation Information 25 2 2 4 Galaxie Installation Procedure 25 2 2 5 Installing the 900 LC Series HPLC Driver 27 2 2 6 Installing the 900 LC Series HPLC Help 29 2 2
32. ctions counters and tests performed on your HPLC system The saved file can be found in your My Documents folder on the PC the system test was performed or in the Log Book tab in the IMS software It is recommended you save those results as a record of your instrument performance at installation If you purchased a separate AIQ package please refer to the documentation provided with the AIQ package for more information Customer Training During the installation process you will receive basic training on how to e set up the hardware e use the Galaxie software e use the Online Help e use the IMS software Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 2 7 Using the Varian 900 LC Series HPLC Online Help The online help is context sensitive Pressing the F1 key on any screen in the device control section of the Galaxie software will bring up help that relates to that screen Alternatively you can access the online help by 1 Click the Windows Start button 2 Click Programs 3 Click Galaxie 4 Click Help 5 Click Varian 900 LC Series HPLC Help 2 8 Instrument Maintenance Software IMS IMS software is used to keep track of any maintenance performed on the HPLC system and usage over time of components within each device in your HPLC system IMS has several features e Counters which record component usage e Actions which lists all procedures that can
33. e Windows Start button and choose Programs gt Galaxie Chromatography gt Help gt Galaxie Configuration Manager Systems should only be configured on an acquisition server or a stand alone PC configuration gt Note This setup wizard will allow all Groups Projects and Users defined in Galaxie Configuration Manager to access this system To configure the 900 LC Series HPLC System s Publication no 8510243600 Issue 1 February 2008 Page 29 of 54 Varian 900 LC Series Liquid Chromatograph Page 30 of 54 1 Click Yes to configure a system if you are installing on an Acquisition server or on a Stand Alone system If you choose No the installer will exit 2 The 900 LC Series Setup Wizard window appears Enter in Note the name of the system This is the system name that will be displayed in the browser window of the Galaxie software Ensure you have typed in the correct system name To modify a system name later you must first delete that HPLC system see page 31 and then use this SetupWizard exe to recreate the HPLC system 3 Click on the magnifying glass icon 2 to start the system discovery process This can take up to 5 10 seconds 4 Click the down arrow button in the Instrument field to select Note Note the HPLC system Identify the system by the MAC address which can be found on the back of the HPLC system and in your documentation that came with the HPLC system Once the system is chos
34. e pump causing a high velocity stream of volatile and or toxic liquids e Wear personal protective equipment when you inject samples or perform routine maintenance e Never open a solvent line or valve under pressure Stop the pump first and let the pressure drop to zero e Read and adhere to all NOTES CAUTIONS and WARNINGS in the manual e Always keep the doors and covers closed during operation Ultraviolet Radiation 900 LC Series Liquid Chromatograph systems that use an ultraviolet light source have shielding to prevent radiation exposure to personnel For continued protection e Ensure that any protective lamp covers are in place during operation Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph e Do not look directly into detector fluid cells or at the UV light source When inspecting the light source or fluid cell always use protective eye covering such as borosilicate glass or polystyrene e Ozone can be generated by radiation from source lamps Exposure to ozone can result in severe irritation to the skin eyes and upper respiratory system The maximum permissible exposure level is 0 1 ppm 0 2 mg m ALWAYS ventilate the area surrounding the HPLC system such that the concentration of ozone does not exceed the maximum permissible level All venting must be to outside air never within the building Solvent Hazards The operator should be familiar with the physi
35. ear of the instrument indicates that the product complies with the requirements of one or more EU directives When attached to the rear of the product SP indicates that the product has been certified evaluated to CSA 1010 1 and UL 3101 1 Color Coding The various indicator lights appearing on Varian instruments and associated accessories are color coded to represent the status of the instrument or accessory e A green light indicates the instrument is in normal standby mode e An orange light indicates that a potential hazard is present e A blue light indicates that operator intervention is required e Ared light may warn of danger or an emergency US FCC Advisory Statement This equipment generates uses and can radiate radio frequency energy and if not installed and operated in accordance with the instruction manual may cause interference to radio communications It has been tested and found to comply with the limits for a Class A computing device pursuant to Subpart J of Part 15 of Federal Communications Commission FCC Rules which are designed to provide reasonable protection against such interference when operated in a commercial environment Publication no 8510243600 Issue 1 February 2008 Page 9 of 54 Varian 900 LC Series Liquid Chromatograph Operation of this equipment in a residential area may cause interference in which case the user will be required to take whatever measures may be necessary
36. en IP address information will be automatically entered into the IP address Subnet mask and Default gateway fields This information will be what the instrument was shipped with and may not be appropriate for your use 7 Choose Obtain an IP address automatically DHCP or Use a static IP address a When Obtain IP address automatically DHCP is chosen the system will search for an IP address automatically If one is not found click Retry The Switching to DHCP mode window pops up Click Initialize instrument This will reboot the HPLC system communications card Click Close when the initialization is complete If an IP address still does not appear contact your IT department to check your network connections The HPLC systems must be on the same gateway subnet as the acquisition server or stand alone PC configuration and be connected to the provided Ethernet switch b When Use a static IP address is chosen enter in the IP address Subnet mask and Default gateway for the HPLC system The HPLC systems must be on the same gateway subnet as the acquisition server or stand alone PC configuration and be connected to the provided Ethernet switch Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 10 11 c Note 12 13 Click Create Click OK when the message confirming the system creation comes up Create up to three more systems or click Close to exit the insta
37. er and allow the column s to come to the correct temperature if necessary Caution It is imperative that whenever the column heater is turned on the pump should be running with a non absorbing solvent flowing through the column to avoid column degradation 9 Caution Turn the detector lamp s on It is imperative that whenever the Varian detector lamp is turned on the pump should be running with a non absorbing solvent flowing through the cell with no air bubbles present For more information on getting your system prepared for use please see the 900 LC Series HPLC Help 1 2 3 4 5 6 7 Click the Windows Start button Click Programs Click Galaxie Click Help Click Varian 900 LC Series HPLC Help Click the plus sign next to How To Click Prepare the HPLC System For Use 3 4 Configuring the Device Settings and System Parameters The Device Settings allow you to program settings for each component within your 900 LC Series HPLC as well as control flow rates purge the pumps turn the detector lamps on heat the column oven and move the autosampler needle and fraction collector probe The System Parameters are settings that will download directly to your method They include information such as the pressure units Publication no 8510243600 Issue 1 February 2008 Page 41 of 54 Varian 900 LC Series Liquid Chromatograph 3 4 1 3 4 2 3 4 3 Page 42 of 54 to be displ
38. f 54 Publication no 8510243600 Issue 1 February 2008
39. g your instrument you will have been provided with a 900 LC Series Liquid Chromatograph Pre installation Manual publication number 8510243900 which describes the environmental and operating requirements of the HPLC system You must prepare your laboratory according to these instructions before the HPLC system can be installed You should keep the pre installation manual for future reference If you have misplaced your copy you can obtain a free replacement from your local Varian office Alternatively download a PDF from the Varian Inc Web site www varianinc com 1 2 User Documentation You have been provided with the following documentation to help you set up and operate your Varian 900 LC Series Liquid Chromatograph e This Getting Started manual with safety practices and hazards information where to find information about installing and maintaining instrument components and a brief operation overview e An extensive online Help system containing context sensitive help step by step instructions for frequently performed operations and instructions for using any accessories you ordered 1 2 1 Conventions The following conventions have been used throughout the documentation e Menus menu options and field names e g select Copy from the Edit menu have been typed in bold Bold is also used to Publication no 8510243600 Issue 1 February 2008 Page 11 of 54 Varian 900 LC Series Liquid Chromatograph 1 2 2 1 3
40. hase AC 3 wire system active neutral ground or two active and ground and should be terminated at an appropriate connection receptacle that is within reach of the system power cable 1 3 2 1 900 LC Series HPLC System Power Connections Power supply current rating Between 3 and 5 A for 220 240 V countries and overload protection Between 6 and 12 A for 100 120 V countries Plug supplied Required wall socket Complies with AS3112 o comply with AS3112 T henero opaan eov 0n USA Complies with Nema 5 15P o comply with Nema 5 15R ee 5262 125 V 15 A Canada Complies with Nema 5 15P To comply with Nema 5 15R Peer with CS 22 2 No 42 Hubbell 5262 125 V 15 A E eee O 250 V 10 16 A Table 2 Power connections 1 3 2 2 Fuses 1FS1 amp 1FS2 T10 A H250 V 3AB SLO BLO for 220 240 V countries T20 A H250 V 3AB SLO BLO for 100 120 V countries FS3 T2 5 A H250 V 5x20 mm for both 100 120 V and 220 240 V countries Table 3 Fuses gt Note For safety reasons any other internal fuses or circuit breakers are not operator accessible and should only be replaced by Varian authorized personnel Fuse information on the rear of the instrument is the most up to date Publication no 8510243600 Issue 1 February 2008 Page 13 of 54 Varian 900 LC Series Liquid Chromatograph 1 3 2 3 Electrical Requirements The installation of electrical power supplies must comply with the rules and or regu
41. in applications demanding high usage of corrosive materials an external cooling air supply system is provided It is recommended that the cooling air be supplied from an environmentally controlled area that is away from the instrument exhaust and any other area where corrosive materials are stored or used ieee and Dimensions Varian 920 LC ee Liquid Chromatograph 750 mm 725mm 665 mm 115 kg 30 in 29 in 27 in 254 Ib Varian 940 LC Semi Preparative Liquid Chromatograph 750 mm 725 mm 665 mm 125 kg 30 in 29 in 27 in 276 Ib Varian 940 LC Semi Preparative Liquid Chromatograph 750 mm 725 mm 856 mm 130 kg with Scale Up Module 30 in 29 in 34 in 287 Ib Varian 940 LC Preparative Liquid Chromatograph 750 mm 725mm 665mm 115 kg 30 in 29 in 27 in 254 Ib Shipping dimensions for all HPLC systems 950 mm 895 mm 1165 mm 170 kg 38 in 36 in 46 in 375 Ib Page 16 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Computer and Printer Computer typical 450 mm 770 mm 520 mm n a 18 in 30 in 20 in Laser printer typical 500 mm 650 mm 200 mm n a 18 in 30 in 20 in External Options Devices Fraction Collector 400 mm 600mm 1480 mm 18 kg 16 in 24 in 19 in 34 Ib Shipping dimensions 760 mm 500 mm 600 mm 31 kg 30 in 20 in 24 in 69 Ib Refractive Index Detector 296mm 385mm 212mm j11kg 12 in 15 in 9 in 25 Ib Shipping dimensions 775 mm 475 mm 460 mm 14 kg 31 in 19 in 18 in 31 Ib
42. ing Installation and Serial Numbers Used During Installation The table below shows which components may need to be installed during the installation process and which serial numbers Publication no 8510243600 Issue 1 February 2008 Page 23 of 54 Varian 900 LC Series Liquid Chromatograph you will need to use New Galaxie Data Chromatography software and new 900 LC Series HPLC driver Existing Galaxie CDS existing Varian HPLC driver and new Varian New Galaxie CDS new Varian HPLC driver and new Varian 900 LC 900 LC driver driver l Galaxie CDS x x T X Galaxie Varian 900 X N A X LC Driver p n 8510244200 Galaxie Varian N A X x HPLC Driver This serial number is used during the installation of the new 900 LC driver p n 0395000491 This serial number is used twice during the installation of both the Varian HPLC driver and the 900 LC driver p n 0395000491 2 2 2 Page 24 of 54 Table 8 Software installation requirements and serial numbers Additional instrument licenses may need to be purchased to run both an existing Varian HPLC and Varian 900 LC instrument at the same time Please contact your local Varian representative for more information Galaxie CDS will need to be installed if a new client acquisition or server is to be used If you are installing both the Varian HPLC driver and the Varian 900 LC driver on the same acquisition server the same seri
43. involves e Setting up the HPLC system to PC communications e Installing the Galaxie software e Installing the Driver and Online Help e Installing the hardware e Running the system test e Basic customer training 2 1 900 LC Series HPLC System to PC Communications The company network must allow TCP IP communications between the client acquisition server and HPLC systems However a desktop switch is provided with the instrument to allow local networking The following are suggested network connections using the provided switch Publication no 8510243600 Issue 1 February 2008 Page 19 of 54 Varian 900 LC Series Liquid Chromatograph Dedicated Subnet for 900 LC s Optional Standard Ethernet Cable Standard Ethemet Cable i Q Ethernet Switch Galaxie _ Company netwo e f Standard Ethernet Cable 900 LC Figure 1 Single 900 LC Series HPLC System in standalone configuration with network connection Standard Ethernet Cable f N Ethernet Switch Galaxie Standard Ethernet Cable 900 LC Figure 2 Single 900 LC Series HPLC System in standalone configuration with no network connection You must use a Static IP address in this configuration Page 20 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Dedicated Subnet for 900 LC s E Optional Standard Ethernet Cable
44. ive 2 Click Next on the Welcome screen The updater will install and update the required files 3 Click Yes to restart your PC Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 4 Click Finish to restart the PC When the restart has completed a window will appear asking for the Main server name if you are performing a Client Server installation 5 Enter the main server name into the field gt Note You must update all PC s in the Galaxie network Always update the main server first AAAA Hot Tip To familiarize yourself with the Galaxie software after installation click the Windows Start button and choose Programs gt Galaxie gt Galaxie Help 2 3 About the Varian 900 LC Series HPLC Online Help Throughout this manual references are made to the Varian 900 LC Series HPLC online help or Galaxie Chromatography Data Systems help The Varian HPLC system online help is provided on a separate CD to the Galaxie software and is installed on your PC during the Galaxie software installation The HPLC system help includes information and instructions on e Safety e HPLC system standard and optional hardware e Installation of the HPLC system options and components including the e Hardware e HPLC system driver for the Galaxie software e Creating methods e Viewing run details e Saving the run e Maintenance e Troubleshooting e Spares and options
45. k cabling and hardware plugged in The 900 LC Series HPLC is an Ethernet device and must be assigned an IP address Contact your IT department to determine if you will be setting up the instrument as a dynamic or static IP and if you will be setting up a client server system or a standalone system The IP address of the 900 LC Series HPLC system must be on the same subnet as the acquisition server or stand alone PC In addition the HPLC systems must be connected to the provided Ethernet switch Disable the Windows firewall on the PC s to be used with Galaxie software This will help prevent installation and data collection problems Prior to installation check the following e Check that power management of your Ethernet network card is set to never allow the card to be turned off Consult your PC manual for instructions e Inthe Windows Control Panel gt Display gt Screen Saver set the power scheme for your monitor and hard drive to Never e Turn off the screen saver e Turn the Windows XP Pro SP2 firewall off e Disable any virus scans e Turn off Automatic Updates in the Windows Control Panel Installing the Galaxie CDS Core Software Note To install the Galaxie CDS core software 1 Please see the Galaxie Chromatography Data System Installation Guide p n 03 914948 02 for installation instructions The 900 LC Series HPLC Driver requires Galaxie software version 1 9 SP1 or greater During the Gala
46. lations imposed by the local authorities responsible for the use of electrical energy in the workplace All power supplies for the 900 LC Series Liquid Chromatograph and accessories should be single phase AC 3 wire systems active neutral ground or two active and ground Each connection should be terminated at an appropriate receptacle within reach of each assembly s power cable Use of power boards or extension cables is not recommended Avoid using power supplies from a source that may be subject to electrical interference from other services such as large electric motors elevators centrifuges and air conditioning units The Varian 900 LC Series Liquid Chromatographs are supplied with a 2 meter long 6 ft 6 in mains supply cable System unit Required supply voltage Nominal rating 920 LC 920 LC Analytical Liquid Chromatograph Liquid Chromatograph 100 120 220 240 V AC 50 60 Hz 1000 VA 940 LC Semi See a Liquid 100 120 220 240 V AC 50 60 Hz 800 VA Ponce hae Table 4 Power requirements 1 3 3 Gas Supplies The installation of compressed or liquid gas supplies must comply with the rules and or regulations imposed by the local authorities responsible for such use in the workplace The main gas supply requirement is nitrogen for supply to ELS Detector Nitrogen from a cylinder or nitrogen generator may be used with Varian 900 LC Series Liquid Chromatograph ELS Detector Page 14 of 54 Publication no 8510243600
47. ller Click Yes or No to the question Do you want to give the right Allow to run instrument maintenance software to ALL of the Galaxie user profiles This will provide access to the Instrument Maintenance Software IMS for all profiles See page 35 for more information on IMS For most unregulated installations click Yes To configure IMS access for individual profiles use Galaxie Configuration Manager If Yes was clicked in step 11 click OK at the All user profiles have been changed window Click Finish on the InstallShield Wizard Complete window 2 2 8 Deleting a System If the system needs to be renamed or the IP address changed you will need to delete the existing system and recreate it To delete a system 1 2 D a Aa Q Open Galaxie Configuration Manager Click the Windows Start button and choose Programs gt Galaxie gt Galaxie Configuration Manager Log in to Galaxie Configuration Manager Click Systems in the upper left side of the window Right click the system to be deleted and choose Delete Click Communication engine configuration on the lower left side of the window Highlight the first line with the system name to be deleted and then click Remove on the upper right side of the window Click Yes to delete the hardware configuration Repeat the previous step for all entries prefaced with the system name to be deleted Publication no 8510243600 Issue 1 February 2008 Page 3
48. nstalling the 900 LC Series HPLC Help This step is a continuation from the previous section Installing the 900 LC Series HPLC Driver To install the 900 LC Series HPLC Help 1 Remove the driver CD and insert the 900 LC Series HPLC Help CD when instructed Click OK If you click Cancel a message will appear confirming your choice not to install the Online Help Click No to exit the installation or click Yes to continue installing the Online Help If the CD Windows Explorer window appears close it Click OK to continue the installation 3 Click Next on the Welcome to the Varian 900 LC Series HPLC Help Setup Wizard window gt Note This window may be behind the 900 LC Series system configuration window Do not close the 900 LC Series system configuration window Move it out of the way until it is needed 4 Click OK to accept the default installation location or choose a different location to install a shortcut to the Online Help 5 Click on Finished 2 2 7 Configuring the 900 LC Series HPLC System s This step is a continuation from the previous section Installing the 900 LC Series HPLC Help To use this setup wizard after the installation process access this setup program by navigating to C Galaxie Server Devices SetupWizard exe Double click SetupWizard exe to launch the setup program or please see the Galaxie Configuration Manager online help To access the Galaxie Configuration Manager Online Help Click th
49. o four HPLC systems connected to one PC Due to this flexibility in the software you must connect to a system when you open the Galaxie program To connect to a system 1 Click the Systems tab at the bottom left of the main Galaxie window 2 Select the empty box next to your system Your Galaxie software will automatically begin communicating with your HPLC system There will be a delay between choosing your system and the system information appearing on your screen The Galaxie software is communicating with the HPLC system Preparing the System for Use For consistent and reproducible results each time you use the 900 LC Series HPLC it is recommended that you follow this procedure to prepare your system to run samples To prepare the system for use 1 Check that all tubing to the column s pumps and waste is correctly connected and in the proper position 2 Place all samples into the autosampler tray Cool the tray prior to placing samples into it if necessary 3 Close the HPLC system doors 4 Check that all tubes are placed in the fraction collector if necessary 5 Set the flow rate manually to remove any air bubbles or old solvent from the system 6 Purge the pumps if new solvent is being used Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Purge any air bubbles from the autosampler injection valve if necessary Turn on the column heat
50. oftware and any other tasks required by the user Click OK to save the changes The software has the menu bar at the top which includes File 1 2 3 4 Open Audit trail 4 Save Audit trail W Preview Audit trail B Print Audit trail Quit View Actions Maintenance mode ON Maintenance mode OFF About For detailed information about the IMS software please see the 900 LC Series HPLC online help To access the online help Click the Windows Start button Click Programs Click Galaxie Click Help Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 5 Click Varian 900 LC Series HPLC Help 6 Click Instrument Maintenance Software in the online help table of contents For information on audit trails 1 Click the Windows Start button 2 Click Programs 3 Click Galaxie 4 Click Help 5 Click Varian 900 LC Series HPLC Help 6 Click the plus sign next to Instrument Maintenance Software in the online help table of contents Click Audit Trails N Publication no 8510243600 Issue 1 February 2008 Page 37 of 54 Varian 900 LC Series Liquid Chromatograph This page is intentionally left blank Page 38 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Operation You will find step by step instructions for common operations in the 900 LC Series HPLC Help To access
51. porates covers that are designed to prevent inadvertent contact with any potential hazards If the instrument is used in any manner not specified by Varian this protection may be impaired It is essential that no cover is bypassed damaged or removed Publication no 8510243600 Issue 1 February 2008 Page 1 of 54 Varian 900 LC Series Liquid Chromatograph Page 2 of 54 The safety practices described below are provided to help the user operate the instrument safely Read each safety topic thoroughly before attempting to operate the instrument and ALWAYS operate the liquid chromatograph in accordance with these safety practices Follow these safety practices to ensure safe equipment operation e Perform periodic leak checks on all supply lines and pneumatic plumbing e Do not allow liquid lines to become kinked or punctured Place lines away from foot traffic and extreme heat or cold or ignition sources e Store organic solvents in fireproof vented and clearly labeled cabinets so they are easily identified as toxic and or flammable materials e Keep the waste vessel in view and empty as required e Do not accumulate waste solvents Dispose of such materials through a regulated disposal program and not through municipal sewage lines This instrument has been tested per applicable requirements of EMC Directive as required to carry the European Union CE Marking This equipment may be susceptible to radiation interference level
52. r use with most HPLC solvents Always check that your HPLC system tubing is compatible with your solvents A chemically inert container not glass and not sealed to hold a minimum of 2 liters 4 pints of waste must be provided by the user Narrow necked containers are preferred The waste vessel s should be bonded It should be placed where it is protected by the bench and in full view of the operator Publication no 8510243600 Issue 1 February 2008 Page 15 of 54 Varian 900 LC Series Liquid Chromatograph 1 3 5 Laboratory Conditions The area selected for the operation of a Varian HPLC system must be free from drafts corrosive atmospheres and vibration Sample preparation areas and materials storage facilities should be located in a separate room The area should be a dust free low humidity environment Air conditioning is strongly recommended for control of the environment For optimum analytical performance it is recommended that the ambient temperature of the laboratory is between 20 and 25 C 68 and 77 F 1 3 6 Instrument Cooling Air Supply Air is drawn from the front of the instrument and out the back to cool the electronics of the instrument Several of these assemblies contain parts prone to corrosion The introduction of cooling air contaminated with high levels of acid vapors or other corrosive substances may cause damage to the instrument Due to the corrosive nature of some work it is recommended that
53. s or frequencies which are not within the tested limits Electrical Hazards Exposure to high voltages and UV energy can cause severe skin damage and cataracts of the eyes while close contact with the electrical components can result in severe heat burns to the skin and an electrical discharge which may cause death severe electric shock or sub surface skin burns The 900 LC Series Liquid Chromatograph has been carefully designed to operate safely and effectively when using components that conform to Varian s design criteria Use of non approved components in the HPLC system may render the system inoperative and or hazardous It may also invalidate the warranty on the instrument Use only related components supplied or authorized by Varian e Disconnect the instrument from all power sources before removing protective panels to avoid exposure to potentially dangerous voltages Panels or covers which are retained by screws on the HPLC system may be opened ONLY by Varian trained Varian qualified or Varian approved Representatives Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Consult the manuals or product labels supplied with your PC to determine which parts are operator accessible When it is necessary to use a non original power cord plug make sure the replacement cord adheres to the color coding and polarity described in the manual and all local building safety codes Good
54. s that can result in death serious personal injury or damage to equipment Always ensure that laboratory safety practices governing the use handling and disposal of hazardous materials are strictly observed These safety practices should include wearing appropriate safety clothing and safety glasses Airflow to the cooling fans of the liquid chromatograph must be unobstructed Do not block the ventilation grills on the liquid chromatograph and accessories Consult the manuals supplied with your PC monitor and for their specific ventilation requirements Great care should be taken when working with glass or quartz parts to prevent breakage and cuts Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph The 900 LC Series Liquid Chromatograph weighs approximately 130 kg 287 Ib To avoid injury to personnel or damage to the instrument or property always use appropriate lifting procedures to move the instrument Use only Varian supplied spares with your instrument Warning and Caution Messages A Warning message is used in the text when failure to observe instructions or precautions could result in death or injury The list of symbols that appear in conjunction with warnings are detailed in the next section A Caution message is used when failure to observe instructions could result in damage to equipment Varian supplied and or other associated equipment Publication no 8510243600
55. te For more information on how to edit report styles please see the Galaxie Chromatography Data System Online Help 3 Click File gt Print to generate a report as specified 4 The Print dialog box will open You can select to print all of the report or a range of pages Reports are printed to your default printer unless you specify otherwise You can specify your printer options in the Print Setup dialog box accessible from the File menu As the Print dialog is a standard Windows dialog you can obtain What s this help on an item by clicking the question mark icon in the top right corner and then clicking the item of interest 3 8 Saving Your Files There are two ways to save a file Publication no 8510243600 Issue 1 February 2008 Page 47 of 54 Varian 900 LC Series Liquid Chromatograph e Save a file with the same name overwriting the previous one by using the menu File gt Save Save file type e Save the file with another name by using the menu File gt Save As Save file type As In both cases the Galaxie Software prompts as default to save the file type corresponding to the active file The Save file window displays the list of the files already created in the directory associated with the group when the user is logged on to All projects or with the project when the user is logged on toa specific project Access the sub directories by double clicking on the corresponding folder icon Once
56. tion due to static electricity when using flammable chemicals Use a conductive vessel and carry out grounding correctly especially when using a non conductive chemical This instrument is not explosion proof Warning Always check the flow path for leakage of solvent or waste solution during use and leakage of solvent inside the instrument If an abnormality is found stop the operation immediately Provide sufficient ventilation in the room Use of the HPLC system and accessories may involve materials solvents and solutions which are flammable corrosive toxic or otherwise hazardous Careless improper or unskilled use of such materials solvents and solutions can create explosion hazards fire hazards toxicity and other hazards that can result in death serious personal injury or damage to equipment gt Note This section refers only to maintenance procedures for the instrument You should refer to your PC and printer manuals for their maintenance procedures and to the 900 LC Series HPLC Help for the maintenance procedures for any accessories you ordered 4 1 Routine Maintenance Routine maintenance information is provided in the 900 LC Series HPLC online help To access these instructions 1 Click the Windows Start button 2 Click Programs 3 Click Galaxie Page 50 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 4 5 6 7 4 2 Cleaning Cli
57. to correct the interference at his or her expense Spurious and Harmonic Radiation Compliance Your Varian 900 LC Series Liquid Chromatograph complies with the requirements of FCC rules and Regulations part 18 subpart H Miscellaneous Equipment at the date of manufacture CE Compliance Your Varian 900 LC Series Liquid Chromatograph has been designed to comply with the requirements of the Electromagnetic Compatibility EMC Directive and the Low Voltage electrical safety Directive commonly referred to as the LVD of the European Union Varian has confirmed that each product complies with the relevant Directives by testing a prototype against the prescribed EN European Norm standards Proof that a product complies with these directives is indicated by e The CE Marking appearing on the rear of the product and e The documentation package that accompanies the product containing a copy of the Declaration of Conformity The Declaration of Conformity is the legal declaration by Varian that the product complies with the directives listed above and shows the EN standards to which the product was tested to demonstrate compliance It is also signed by Varian s Authorized Representative in the EU and by the representative of the manufacturing plant Page 10 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 1 Introduction 1 1 Pre installation Requirements Prior to receivin
58. use Two PCl compatible slot for I O card Two PCl compatible slot for I O card Windows XP SP 1 Windows XP SP 1 Microsoft Internet Explorer v6 0 Microsoft Internet Explorer v6 0 Table 7 Recommended PC specifications For Galaxie CDS server specifications please contact your local Varian sales representative PCs supplied with Letter of Credit orders will be an international brand and will be the Recommended configuration or better Higher rated PC components can be substituted for those listed above e g processor type amount of memory screen size and resolution operating system version etc For the latest PC and video card recommendations please see the Varian Inc website at www varianinc com Page 18 of 54 Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph 2 Installation The Varian 900 LC Series Liquid Chromatograph must be installed by a Varian trained Varian qualified or Varian authorized representative You should have completed and returned the form in the pre installation manual stating that you have prepared the laboratory in accordance with the requirements detailed in that manual A Varian representative will then arrange a suitable installation date with you Details for unpacking the instrument and what to do in case it has been damaged in transit are also outlined in the pre installation manual Basic installation
59. xie software and Galaxie Configuration Manager is installation a default profile is created The user name admin and password galaxie can be used to log into Galaxie Configuration Publication no 8510243600 Issue 1 February 2008 Varian 900 LC Series Liquid Chromatograph Manager to set up users systems and permissions and into the Galaxie software to run the system This user name and password can be changed in Galaxie Configuration Manager 2 2 4 2 Installing Galaxie or Other HPLC or GC Device Drivers The drivers work with the Galaxie core software to provide instrument control and data acquisition These instructions will provide information to install drivers other than the 900 LC Series HPLC driver Go to the next section Installing the 900 LC Series HPLC Driver if this procedure is not required To install Galaxie or other HPLC or GC device drivers 1 Please see the Galaxie Chromatography Data System Installation Guide p n 03 914948 02 for installation instructions 2 2 9 Installing the 900 LC Series HPLC Driver The drivers work with the Galaxie core software to provide instrument control and data acquisition Install this driver on the main server and each acquisition server and client PC that will be working with the 900 LC Series HPLC system gt Note If you have purchased the single system Galaxie software license open Galaxie Configuration Manager and delete the Default system that is created during the
60. y a Thru Pump Injector Autosampler If you purchased an analytical or analytical to semi preparative system you may have an autosampler In the Autosampler Device Settings you can move the needle to the e wash port e injection port You can also flush the syringe barrel to replace solvent or remove bubbles by clicking the Flush Syringe Barrel button If you have the cooling option on your autosampler you can change the temperature Manual Injector Depending on the options you chose you may have a manual injector This allows you to make single injections The Device Settings window lets you make an injection and shows you the state of the injector Injection Pump The preparative scale HPLC system will have an injection pump In the injection pump method you can program the injection volume and flow rate 3 4 5 System Parameters System Parameters are settings that affect new methods If you change the System Parameters while a method is already open the changes will not affect that method Depending on the system you purchased you can e set the units preferred for the pressure and set the cell ratio if using a semi prep or prep cell e edit the manual injector options including the reset time analytical SUM loop size and preparative loop size e edit the reset time for a manual injector which is used to switch the valve back into the idle position e enable the rack cooling option in the autosampler section

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