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YSI 2700 SELECT User`s Manual

1. In the fields above the characters represent the following current system time hh mm ss 24 hour clock current system date mm dd yy or dd mm yy depends on format not used Always comm node address if multidrop Blank for point to point moO PS measurement parameter report ID Always 3 black channel chemistry abbreviation black channel calibration standard value black channel calibration concentration unit black channel calibration endtime 15 to 90 seconds F G H I J black channel calibration station number 1 to 5 white channel chemistry abbreviation white channel calibration standard value white channel calibration concentration unit white channel calibration endtime 15 to 90 seconds OZEUTR white channel calibration station number 1 to 5 Figure 9 6 RM Command Report Format 9 15 When you invoke the RI Report Information command the instrument uploads the instrument setup that is not reported by the RM command except communications and local control parameters The information reported by this command is coded to conserve space You must know the codes listed in Figure 9 7 to understand these setup parameters The report is divided into several categories similar to PrntSetup Print Setup in the 2700 SELECT menu option Note all fields in this report are right justified 123456789 123456789 123456789 123456789 123456789 123456789 1
2. AutoCal Figure 5 2 YSI 2700 SELECT Menu Flow Chart REMEMBER You move through the 2700 SELECT menus by selecting options on the display You can press MENU to get back to the Main Menu and 0 to back up to the previous display However it may be necessary to confirm a response by pressing ENTER before continuing to use the MENU or 0 keys 5 2 Service Selections Service functions are primarily used during initial setup and anytime reagent changes are made Both Cal Pump and Buffer Pump can be primed using this menu level In addition Sipper alignment and Stir Bar speed adjustment can be performed from this level Use the display messages and text below to learn the service functions Main Menu display Please select instrument mode RUN STANDBY MENU From Main Menu press MENU to display instrument functions Select instrument function 1 Service 2 Setup 3 Diagnostic Press 1 for Service to display service functions Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable Press 1 for Sipper to initiate Sipper alignment This procedure was used during initial instrument setup Refer to Section 2 9 for more information The Sipper Arm Assembly will move to the home position Loosen the adjustment screw that holds the Sipper flange to the arm to center the Sipper over the Sample Chamber injection port The displa
3. Either enter another address or accept the default then confirm with ENTER The node address is only used when networking 2700s in a multi instrument system The 2700 responds only to commands prefixed with the ID matching the address assigned from this menu 5 7 Now press 0 to return to the General setup level General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor on Press 4 for Radix In some parts of the world a is preferred to express decimal numbers For example 2 00 2 00 Confirm your choice by pressing ENTER to return to the General setup level Select radix mark here Dae General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 5 for Serial The serial of your instrument is recorded on the serial plate lower rear of case Enter this number in memory It will be printed for certain report formats NOTE YSI uses alpha characters in the serial number We recommend that you press SPACE for the first alpha character and omit the last two Example 98B01234AB can be entered as 98 01234 This number is normally set prior to delivery and preserved in memory by battery backup Press ENTER to confirm and return to General setup level Enter instrument serial number 98 01234 General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 6 for Level Sensor Choose Yes to maintain level sensing in the b
4. Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default From Select setup press 5 for PrntSetup to print all of the instrument parameters currently in memory If you repowered the instrument after replacing batteries or reset the instrument using the reset button the printout first shows an instrument identification including the software version number Once you command the instrument to Print Setup the Instrument Setup prints out on the paper tape This takes about 60 seconds The information on the printout completely describes the system parameters whether using default settings or settings that you configured for your particular application You can learn about the system parameters by referring to Section 5 3 Setup in this manual If you have a YSI 2710 Turntable refer to Section 3 in the 2710 Turntable Operation Manual Printed Setup Information The printouts for single channel and dual channel 2700 s are shown on the following page Note if a calibration or sample data report is due to print while you are performing PrntSetup the instrument will interrupt PrntSetup to print results PrntSetup printing then resumes automatically C 1 C 2 INSTRUMENT I SETUP Sample size SamStation 25 uL 2 Cal Method One station ACK PROBE hemistry nit alibrator End Point CalStation AUTOCAL SampleError Temperature Time Sample Cal Shift RUN MODE
5. g L 0 50 30 Sec 1 Dextrose g L 2 50 30 1 15 5 Sam OFF OFF Medium 2 Hr ON Min Min Min Sec 9600 Seven bit One bit Even RTS CTS 17 19 multidrop ON Brief Brief MM DD YY sion 2 56 8 01234 09 22 36 Appendix D Report Formats When you setup your YSI 2700 SELECT from the Setup Menu you have the option to print the calibration and sample results in brief or detail format You also have the option not to print calibration values Review Section 5 3 Setup specifically choice 4 Report in the Setup selection menu For more specific information on the detail printout refer to Section 8 Troubleshooting Example printouts of both formats are presented below These examples show printed results of samples and calibrations In the first Sample Report the ID 123456789 05 03 indicates that the third of five replicates is being reported for the sample identified by the nine digit number 123456789 Sample Report Detail Sample Report ID 123456789 05 03 B L Lactate 4 82 g L IB 2 11 nA PL current 18 65 nA Slope 0 69 nA min End Point 30 Sec W Dextrose 55 38 g L IB current 0 88 nA PL current 15 96 nA Slope 0 39 nA min End Point 30 Sec Temperature 26 28 C Sample size 25 uL Fri 02 27 98 08 12 34 YSI 2700D 98 01234 Sample Report Brief ID 123456789 Replicate 1 of 3 Sample Report ID 1234
6. 2 6 Calibrator solution out of spec contaminated or installed for more than 30 days Install new calibrator 2 3 Net calibration current PL current below 5 nA Replace enzyme membrane and check calibrator solution 2 4 SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION ERROR Unable to Calibrate Continued Failing enzyme membrane Perform daily operational checks and replace membrane s if necessary 3 4 Probe surface fouled Clean probe surface 7 5 Temperature Probe auxiliary electrode fouled Clean probe surface 75 ERROR Undetectable Fluid Low calibrator solution Install new calibrator 2 3 Pinched blocked leaking or disconnected tube Fix or install new tubing 7 6 Calibrator pump not operating properly Replace tubing 7 6 Fluid not conductive Use saline as diluent 4 8 Low sample level Run sample at manual position Blank Printout Printing on wrong side of paper Install paper correctly 2 6 Using wrong type of paper Install correct thermal paper YSI 2751 2 6 F
7. Operation at the Test Tube Holder Station 2 The Test Tube Holder See Figure 3 2 accepts tubes from 9 to 16 millimeters in diameter and 50 to 100 millimeters long Any container other than a tube with the dimensions indicated should be presented at the Manual Station Station 3 Some double wall plastic tubes may prevent the fluid detection from working properly These tubes should also be presented at the Manual Station For the purposes of demonstrating basic operation choose the high level standard for the chemistry you are set up to run For example if you have installed a YSI 2365 glucose oxidase membrane calibrate with YSI 2776 standard and use YSI 1531 standard containing 9 00 g L dextrose to run as a sample Using the appropriate standard from your starter supplies fill a test tube about half full The Test Tube Holder is hinged at the bottom Pull out the top as shown in Figure 3 3 Place the tube in the holder The Sipper is not designed to pierce septa but can be used with some flexible evaporator covers that are preslit Alternatively you may use a smaller container and sample from the Manual Station 3 to conserve linearity standard solution Push the holder back into place The Test Tube Holder Sensor located above the holder detects the presence of a tube If a tube is detected the Sipper will sample from this station if no tube is present it will move to the Manual Station Station 3 Press SAMPLE The 270
8. Press 2 for Setup The instrument now displays 6 categories as shown below Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default During normal operation you will seldom need to enter the Menu Mode but it is important to familiarize yourself with the menu locations of the various parameters over which you have control See Section 5 3 for complete details of menu options Press 5 for PrntSetup The data printer will print the instrument setup parameters This will take less than a minute and use approximately 8 inches 20 centimeters of printer paper The hard copy of the current parameters setup should help you as you work through this menu Each category of the Setup Menu is briefly described below For now study the Setup categories A step by step set of instructions that leads you through an example setup will follow Press 1 for General The display will read General setup 1 Date Time 2 Contrast 3 RS 232 4 Radix 5 Serial 6 LevelSensor 1 General In this menu you may confirm or change the date set date format adjust the display contrast define communication parameters select the radix to express decimal numbers confirm or enter the instrument serial number and select whether to deactivate the bottle level sensor system Press 0 to back up then press 2 for MeasParameter The display will read Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White 2 MeasParam
9. When Report Sample Result RS is invoked the 2700 sends back the last sample processed When combined with an ID number the 2700 searches its database and sends back the most recent matching ID result After the result has been transmitted its entry in the database is marked as sent Another request for the same sample ID result will cause the 2700 to send the next most recent result for the matching ID result If no result is found the 2700 sends back error code 9 If the host has received the result in error due to a data transmission error it should request retransmission using the RX command Host to 2700 RS Report last sample result 2700 to Host sam result see format in Figure 9 4 Host to 2700 RS ID Report sam ID result max 9 digits 2700 to Host sam result see format in Figure 9 4 9 11 Remote Control Commands The commands outlined above in the Process category can be invoked only in Remote Control Mode If the system is not in the Remote Control Mode you must first invoke the Turn Remote Control On TR1 command Note For each command from Host to 2700 the 2700 sends back an acknowledgment A or an error code Error codes may require invoking the Report Status RY command to gain further information on machine status Host to 2700 TRI Turn on remote control mode 2700 to Host A Acknowledge 2700 switched to Remote Control 2 2700 busy in Run Mode 9 2700 is in a halt condition page 9 4
10. YSI incorporated YSI 2700 SELECT Biochemistry Analyzer User s Manual Who s Minding the Planet find out t WWW Y SI com Table of Contents Introduction 1 1 Description 1 2 Standard Features 1 3 General Specifications 1 4 Chemistry Performance Specifications 1 5 How to Use this Manual Initial Setup 2 Unpacking and Notes on Safety 2 2 Major Component Identification 2 3 Reagent Preparation 2 4 Enzyme Membrane Installation 2 5 Power Up Procedures 2 6 Fluid System Priming 2 7 Printer Paper Installation 2 8 Instrument Parameter Programming 2 9 Initial Setup Example Step by Step 2 10 Probe Baseline Check Basic Operation 3 1 Main Menu 3 2 Run Mode 3 3 Standby Mode 3 4 Daily Operational Checks Chemistry Setup 4 Principle 4 2 Sample Preparation 4 3 Measurement Parameter Setup Information 4 4 D Glucose Dextrose 4 5 L Lactate 4 6 Sucrose 4 7 Lactose 4 8 Ethanol 4 9 Starch 4 10 Choline 4 11 Galactose 4 12 Hydrogen Peroxide 4 13 L Glutamate L Glutamic Acid 4 14 L Glutamine 4 15 Methanol 4 16 Simultaneous Dextrose and L Lactate 4 17 Simultaneous Dextrose and Sucrose 4 18 Simultaneous L Glutamate and L Glutamine 1 1 1 3 1 4 1 5 1 8 2 1 2 2 2 5 2 6 2 8 2 9 2 11 2 12 2 15 2 25 3 1 3 2 3 7 3 7 4 1 4 2 4 3 4 5 4 6 4 7 4 8 4 9 4 10 4 11 4 12 4 13 4 14 4 15 4 16 4 17 4 18 4 20 Menu Selections 5 1 Introduction 5 2 Service Selections
11. 5 00 g L 25 0 g L 2 2 5 10 days Blue 2771 0 450 mg L 175 mg L 450 mg L 2 2 t5 7 days Orange 2735 0 8 mmol L 5 00 mmol L 8 00 mmol L 4 4 5 5 days Magenta Specs shown are for simultaneous measurement of glutamine and glutamate Methanol YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV n 10 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring color Potential Substrate Interference 2725 0 2 50 g L 1 00 g L 2 50 g L 2 2 or 0 02 g L whichever is greater 5 5 days Black Ethanol NOTE For the following chemistries YSI believes that you will be able to measure these analytes for many applications in the ranges specified below However YSI makes no claims with respect to precision or linearity For each analyte below you will need to prepare the calibrator and linearity standards for your application YSI also recommends that you confirm that no significant levels of interfering substrates are present in your samples For example we know that lactose and galactose in the same sample may interfere if significant concentrations of both exist Also dextrose will read at a sucrose membrane however by using the dual channel approach to dextrose and sucrose this is automatically accounted for by the software Galactose Membrane Detection Range Typical Membrane Working Life M
12. Chemistry Calibration Std Linearity Std Acceptable Range g L Dextrose 2776 2 50 g L 1531 9 00 g L 8 55 to 9 45 Ethanol 2790 2 00 g L 2790 3 20 g L 3 04 to 3 36 Lactose 2783 5 00 g L 2784 25 00 g L 23 75 to 26 25 L Glutamate 2755 0 73 g L 2756 1 46 g L 1 39 to 1 53 L Lactate 2776 0 50 g L 1530 2 67 g L 2 54 to 2 80 Sucrose 2780 5 00 g L 2778 25 00 g L 23 75 to 26 25 Choline 2772 0 175 g L 2773 0 450 g L 0 43 to 0 47 L Glutamine 2736 0 73 g L 2737 1 17 g L 1 11 to 1 23 Methanol 2726 1 00 g L 2726 2 50 g L 2 38 to 2 63 If you are setting up to measure galactose or hydrogen peroxide using one of the YSI Enzyme Membrane Sensors see Section 4 for more information f you use units other than g L for the linearity test refer to Appendix A for conversion values NOTE If any reading is outside of the specified tolerance limits recalibrate and repeat the linearity test If the reading is still out of tolerance refer to Section 8 Troubleshooting 4 Chemistry Setup 4 1 The YSI 2700 SELECT has been designed to provide considerable flexibility with respect to how it may be configured This allows you to use the instrument for analyses other than the standard configurations mentioned previously This flexibility also makes it possible for YSI to develop and introduce new chemistry analyses in the future without significant instrument changes In this section you will learn about each of the che
13. You should invoke this command from Standby or Menu mode If you attempt to enter Run Mode by pressing the RUN key locally then invoke the TR1 command prior to successful calibration you may not successfully enter remote control mode Alternatively you may enter Run Mode locally by pressing RUN and waiting for successful calibration Then invoke the TR1 command to enter remote control mode In a similar manner you can invoke the Turn Remote Control Off TR0 command to return to Result Reporting Mode In the Remote Control Mode you may elect to invoke the Turn 2700 Printer Off command The 2700 responses are outlined below Host to 2700 TPO Turn 2700 printer off 2700 to Host A Acknowledge 2700 printer turned off 1 2700 is not in Remote Control Mode In a similar manner you may use TP1 to turn the 2700 printer on Commands to Exit Enter Run Mode remotely You may use the commands described below to enter and exit Run Mode however always verify that you are in Remote Control Mode prior to invoking these commands When you invoke TNO Exit Run Mode the instrument exits Run Mode and switches to Standby Mode or if there is a halt condition to Menu Mode Standby Mode may be used to conserve reagents in certain applications Menu Mode positions you to remotely recover from most error conditions Host to 2700 TNO Exit Run Mode and switch to Standby Mode or Menu Mode 2700 to Host A Acknowledge 2700 exits Run M
14. you would like to maintain a sample ready status before reverting to STANDBY Mode Press ENTER then MENU to return to the Main Menu Daily Operational Checks To ensure that your 2700 SELECT is operating properly perform the following operational checks on a daily basis It is advisable to maintain an instrument log book in which dates and lot numbers of reagents are recorded along with information from daily operational checks and other relevant information In the log book you may want to paste a printed record of your operational checks Membrane Integrity Test Use YSI 2363 Potassium Ferrocyanide FCN Standard to determine if your membranes are structurally intact This standard is packaged in your starter supplies or may be ordered separately The test is semiquantitative but should offer useful information on membrane leakage NOTE This test is recommended for all YSI Enzyme Membranes except Galactose Oxidase Membranes YSI 2702 In these membranes FCN is a component of the supply buffer High baseline error messages may provide similar information regarding the structural integrity of the installed membrane Place your instrument in RUN Mode When the unit is ready the following display will appear Ready to sample at Station 2 Pour a small amount of FCN Standard 1000 mg dL into a tube or cup and run it as a sample at either the Manual or Test Tube Holder Station The maximum allowable values for FCN readings af
15. 00 mmol L 10 0 mmol L 2 2 X596 7 days Yellow User may configure instrument to measure as monosodium glutamate MSG L Lactate L Lactic Acid YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV nz10 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring Color 2329 0 2 67 g L 0 50 g L 2 67 g L 2 2 5 14 days Gray 1 5 1 6 Lactose YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV n 10 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring Color Sucrose YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV n 10 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring color Choline YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV n 10 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring color L Glutamine YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV n 5 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring color 2702 0 25 g L 5 00 g L 25 0 g L 2 2 5 10 days White 2703 0 25 g L
16. 01 08 Time 02 10 08 Date 03 19 Temperature 25 03 Node Address 05 29 Sample ID 0 noID Cal Report 2 Monitor Sample Report 3 Information Report Probe Chemistry Assignment None no chemistry DEX Dextrose LAC L Lactate SUC Sucrose LTOS Lactose GAL Galactose H202 Hydrogen Peroxide ETOH Ethanol MEOH Methanol GLMT L Glutamate GLMN Glutamine CHOL Choline Sample or Calibration Result Sample or Calibration Concentration Unit Error Code hexadecimal Continuation character i more data follows space end of record Figure 9 5 Report Format Field Information Command Description For quick reference the communications commands are divided into five functional groups process report database database management control and report system information For details on process commands see Remote Control Commands later in this section PROCESS PC Process calibration PS1 Process sample from calibration well Station 1 PS2 Process sample from test tube holder Station 2 PS4 Process turntable batch Station 4 PS5 Process monitor sample Station 5 REPORT DATABASE RC Report calibration result RS Report sample result RS Report sample result with sample ID number RY Report status of instrument RX Report last transmission DATABASE MANAGEMENT RZ Clear database memory CONTROL TRO Turn remote control off TRI Turn remote control on TPO Turn 2700 printer off TPI Turn 2700 printer on MPt Change pump purge
17. 1 12 04 02 13 98 23 56 123 1 H202 45 78 nA 0000N cr 1l 12 04 02 13 98 23 56 123 1 H202 15 28 nA OF01 lt cr gt lt l 02 34 02 13 98 24 86 2 H202 78 mmol L 0000 lt gt lt 1 02 34 02 13 98 24 86 2 H202 78 g L 0000 lt cr gt lt l Figure 9 4 2700 SELECT Data Report Format NOTE The specific information above is not intended to appear realistic but rather to account for all characters and spaces in the report format See Figure 9 5 for specific field location information 9 7 The fields of the data transmission records are more specifically defined below in Figure 9 5 A ruler line is used simply to help you identify the offset and field widths in the report We have used the following symbols to depict changes from early 2700 software vs versions 2 41 and higher The symbol indicates a change The symbol indicates an addition The symbol KE labels the choice NONE to indicate that when this choice is made the remainder of that line fills with blanks Each field is identified by a number series just below the ruler line These field numbers help you visualize column width 123456789 123456789 123456789 123456789 123456789 123456789 123456789 12345 11 11 11 22 22 22 33333 444 555555555 6666 77777777 88888888 9999 lt cr gt lt lf gt 11 11 11 22 22 22 33333 444 555555555 6666 77777777 88888888 9999 lt cr gt lt lf gt FIELD OFFSET WIDTH DESCRIPTIONS NO COLUMN BYTE 01
18. 2 4 Figure 2 5 Figure 2 6 Figure 2 7 Figure 3 1 Figure 3 2 Figure 3 3 Figure 3 4 Figure 5 1 Figure 5 2 Figure 5 3 Figure 6 1 Figure 6 2 Figure 6 3 Figure 6 4 Figure 6 5 Figure 7 1 Figure 7 2 Figure 7 3 Figure 7 4 Figure 7 5 Figure 7 6 Figure 7 7 Figure 7 8 Figure 7 9 Figure 9 1 Figure 9 2 Figure 9 3 Figure 9 4 Figure 9 5 Figure 9 6 Figure 9 7 Figure G 1 List of Figures Inside Front View of the 2700 SELECT Back View of the 2700 SELECT Bottles and Level Sensor Cables Sample Chamber Sensors Enzyme Membrane Installation Line Voltage Selector Sipper Adjustment Position 2700 SELECT Software Structure Sampling Stations The Test Tube Holder Pivoted Out The Manual Station 2700 SELECT Software Structure YSI 2700 SELECT Menu Flow Chart 2700 SELECT Major Components Sensor Probe and Enzyme Membrane 2700 SELECT Fluid System Typical Sensor Response Aging Membrane Response 2700 SELECT Software Structure Sample Chamber Illustration Tubing Connections Pump Tubing installation Pump Assembly Fuse Replacement Sipper Pump Head Removal Sipper Pump Seal Replacement Sipper Pump Plunger Position Sipper Mechanism Drive Screw and Guide Rods RS 232 Signal Description and Direction Full Handshaking DTE Interface Three Wire DTE Interface 2700 SELECT Data Report Format Report Format Field Information RM Command Report Format RI Command Report Format Line Power Cord and Plug Wiring iv 220
19. 2786 Carbonate Buffer Concentrate use with 2725 membrane Lactose Buffer Kit use with 2702 membrane Ethanol Buffer Kit use with 2786 membrane Potassium Ferrocyanide 1000 mg dL NaCl Solution sodium chloride Reagent packaged dry Makes specified volume when reconstituted Reagent concentrated Makes specified volume when reconstituted Miscellaneous Accessories amp Supplies YSI 4 2710 2711 2730 2731 2751 2788 Contact YSI Incorporated or your authorized dealer representative for more information on ordering Product Description Turntable Upgrade pre 1991 YSI 2700 s with turntable interface Monitor and Control Accessory 2730 Preventive Maintenance Kit Printer Paper 5 rolls box Preventive Maintenance Kit supplies and reagents for the YSI 2700 SELECT Quantity 125mL 125mL 125mL 250mL 125mL 125mL Quantity Aliters liter 4liters 4liters 125mL 30mL Appendix C Printed Setup Information You may print your 2700 SELECT instrument setup anytime by entering the Setup menu You are not required to leave RUN Mode or STANDBY Mode to access this information Main Menu display Please select instrument mode RUN STANDBY MENU From Main Menu press MENU Select instrument function 1 Service 2 Setup 3 Diagnostic From Select instrument function press 2 for Setup NOTE If you are in RUN or STANDBY modes press MENU to go directly to the display shown below
20. 5 3 Setup Selections 5 4 Diagnostic Selections Principles of Operation 6 1 Sensor Technology 6 2 Fluid System 6 3 Measurement Methodology 6 4 Baseline Stability 6 5 Calibration 6 6 Linearity 6 7 Temperature Compensation 6 8 Level Sensing and Sipper Interference 6 9 Software Structure Maintenance 7 1 Daily Maintenance 7 2 Calibration Pumping System Maintenance 7 3 Sample Chamber Cleaning 7 4 Membrane Replacement 7 5 Probe Cleaning 7 6 Tubing Replacement 7 Sipper Replacement 7 8 Sipper Ground Cable 7 9 Fuse Replacement 7 10 Sipper Pump Seal Replacement 7 11 Sipper Mechanism Lubrication Troubleshooting 8 1 Printout Information 8 2 Troubleshooting Chart Communications 9 1 Communications Protocol 9 2 Communications Modes 9 3 Communications Commands 5 1 5 3 5 5 5 17 6 1 6 3 6 4 6 4 6 5 6 5 6 6 6 6 6 7 7 1 7 3 7 4 7 5 7 6 7 7 7 9 7 9 7 10 7 11 7 13 8 2 8 4 9 1 9 5 9 6 Appendix A Appendix B Appendix C Appendix D Appendix E Appendix F Appendix G Appendix H Appendix Contents Of Appendices Concentration Unit Conversion YSI Supplies and Reagents Printed Setup Information Report Formats FCC Required Notice Warranty and Shipping Information Line Power Cord and Plug Wiring Cleaning Disinfecting and Decontamination Procedures CE Compliance iii 1 1 1 D 1 F 1 G 1 H 1 I 1 Figure 2 1 Figure 2 2 Figure 2 3 Figure
21. Dextrose 8 3 g L End Point 30 Sec IB current 0 88 nA Cal shift 3 64 PL current 58 96 nA W Dextrose 10 0 g L Slope 2 39 nA min IB current 0 88 nA End Point 30 Sec PL current 5 81 nA Temperature 26 28 9C FB current 0 76 nA Sample size 25 uL Base shift 1 95 Fri 02 22 98 08 12 34 Slope 0 09 nA min YSI 2700D 98 01234 End Point 30 Sec Cal shift 0 10 Temperature 20 33 Sample size 25 uL Thu 02 22 98 14 55 10 YSI 2700D 98 01234 IB current Initial Baseline Current The initial baseline current is monitored before a sample or calibration and while stabilizing baseline current upon entering RUN Mode To successfully enter RUN Mode the IB current must be stable and below 6 nA PL current Plateau Current The minimum acceptable plateau current is 5 nA The maximum plateau current is 500 nA A plateau current larger than this will be printed as a string of asterisks ew FB current Final Baseline Current The final baseline current is printed for calibrations and samples The baseline current is monitored during the buffer flush and compared to the initial baseline current Base shift Baseline Shift The final and initial baselines are compared and reported as percent shift A negative baseline shift is not uncommon with newly installed membranes High concentration samples may yield positive baseline shifts An excessive positive shift can be an indicator of the presence of an interfering substance The message Fin
22. ENTER to confirm and move to the next parameter Enter White end point in seconds gt 30 Press ENTER to confirm the 30 second endpoint parameter Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 0 to return to the Select setup level Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 3 for RunMode RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor Press 1 for SampleProtocol SamplingProtocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 1 for SipperHeight Select manual sipper height Medium 1 Low 2 Medium 3 High This specifies to what vertical position the Sipper descends when sampling at the Manual Station For example with long test tubes and the fluid level near the bottom of the test tube use Low setting For now choose Medium level then press ENTER to confirm and return to Sampling protocol setup SamplingProtocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 2 for Replicates Prompt replicate cycle No 1 No 2 Yes Since our example setup requests triplicate analysis of the sample we must activate replicates Press 2 for Yes Prompt replicate cycle Yes 1 No 2 Yes Press ENTER to confirm and return to Sampling protocol setup SamplingProtocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 3 fo
23. Report The display will read Select sample report format Brief 1 None 2 Brief 3 Detail 4 Report In this menu you select the level of detail you desire for the printed sample and calibration reports You probably would reserve detail reporting for troubleshooting a suspected problem Press ENTER to confirm brief sample report format Select cal report format Brief 1 None 2 Brief 3 Detail Press ENTER again to confirm brief cal report format Press 5 for PrntSetup The display will read Printing instrument setup Please wait 5 PrntSetup This is an abbreviation for print setup Once you have selected your setup parameters you may record these choices by printing them on the data printer You have already been instructed to print this to learn about the 2700 SELECT menu 2 13 Press 6 for Default The display will read Reset all system parameters No 1 No 2 Yes 6 Default In this menu you have the option to reset all default parameters i e those set in the software to serve your needs for all standard applications Using this command also requires that you reset date time and all other general parameters Press ENTER to confirm No and return to Select Setup menu Now press MENU to return to Main Menu display Please select instrument mode RUN STANDBY MENU 2 14 2 9 Initial Setup Example Step by Step Next we will show a step by step instrument programming procedure to illus
24. Representative Appendix F G Do NOT use an extension cord without protective grounding Do NOT remove rear cover There are no user serviceable parts inside 4 5 6 Repairs are to be performed only by trained and approved personnel 7 This instrument must be connected to a protectively grounded earthed outlet 8 The following notices are provided in compliance with IEC1010 Part 1 1990 8 Fuses FI F7 on the main circuit board are type Subminiature F to UL 198G Standard Rating F1 F5 1A F6 F7 2A These fuses are NOT operator replaceable 8 2 See Appendix G for mains plug wiring and fusing instructions 9 If the equipment is used in a manner not specified by YSI the protection provided by the equipment may be impaired WARNING For auxiliary connection refer to the YSI 2710 Turntable Operation Manual or the YSI 2730 Monitor and Control Accessory User s Manual Use with the YSI 2710 Turntable or YSI 2730 Monitor Control Accessory only WARNING For remote connection refer to section 9 Communications Equipment should be EN 61010 or EN 60950 approved only 10 The mains power switch is for functional purposes ONLY To disconnect the instrument from the mains supply unplug the mains power cord from the back of the instrument 2 1 2 2 2 2 Major Component Identification Referring to Figure 2 1 and 2 2 read through the following descriptions and familiarize yourself with the major components NOTE
25. Sample may contain an interfering substance Attempt to confirm interference 4 12 ERROR Empty Buffer Bottle Low buffer level Refill the buffer 2 3 SYMPTOM POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION ERROR Empty Buffer Bottle Continued Level sensor cable not plugged in Plug in cable 2 3 ERROR Empty Calibrator Bottle Low calibrator level Install new calibrator 2 3 Level sensor cable not plugged in Plug in cable 2 3 ERROR Full Waste Bottle High waste level Empty bottle Level sensor cable in contact with instrument housing or another conductive body Reroute cables 2 3 ERROR Motor Failure One of the motors is jammed Enter motor diagnostics and cycle the suspected motor 5 4 Dirty drive screw on sipper mechanism Clean and lightly oil 7 11 Worn sipper pump seals Replace seals 7 10 ERROR Overrange Sample concentration too high resulting in high probe nA Dilute sample or adjust sample size down and repeat 4 3 ERROR Printer Failure Detected Printer paper or roll jam Open paper we
26. Station 3 to Test Tube Holder Station Station 2 NOTE If the Sipper is down and you press a command for horizontal movement the Sipper first moves up before beginning horizontal motion Press 6 to initiate repeated motions in the same plane We refer to this as Exercise The motion horizontal or vertical is determined by the last command you chose The instrument counts the number of cycles and displays them as shown below When you press a key to terminate the exercise mode the printer records the activity and number of cycles completed Exercising sipper motor Cycle 3 Hit any key to stop Press 0 to return to Sipper diagnostic selections Sipper diagnostic 1 Up 2 Down 3 Home 4 Clockwise 5 Counterclock 6 Exercise Press 0 again to return to Motor diagnostic selections Motor diagnostic 1 Sipper motor 2 Stir motor 3 Turntable Press 2 for Stir motor to turn on the motor that spins the magnetic stir bar in the Sample Chamber The bar should spin in synch i e not jump about the chamber as is observed during brief periods of normal operation The asynchronous motion is used to dispel air bubbles from the chamber wall and probe surfaces You turn the Stir Motor off by pressing 2 again The Stir motor may be activated with the Sipper either in or out of the Sample Chamber Motor diagnostic 1 Sipper motor 2 Stir motor 3 Turntable Press 0 to return to the five diagnostic selections
27. Std YSI 1531 and YSI 2778 Black Membrane YSI 2703 White Membrane YSI 2365 Sample Size 25 uL Cal Station Two Station 1 dex 42 suc Black Probe Parameters White Probe Parameters Chemistry Sucrose Chemistry Dextrose Unit of Conc g L Unit of Conc g L Cal value 5 00 Cal value 2 50 End Point 30 sec End Point 30 sec Since sucrose calibrator is at the Test Tube Holder you must sample at the Manual Station 3 The Turntable position 4 and the Monitor Station 5 may also be used on units equipped with these accessories Special Considerations The combined total of dextrose sucrose cannot exceed 25 g L The dextrose concentration cannot exceed 10 g L if the combined total is near 25 g L since a dextrose concentration that high combined with 15 g L sucrose will saturate the Sucrose probe If either of these conditions occur you should dilute your sample to bring it into a reasonable range The ability to select sample size 5 to 65 uL may help you optimize your system especially if very high or very low concentrations of one or the other of these substrates is a problem The optimal probe current for a calibrator is about 10 nA See Menu Diagnostics Section 5 4 to learn more about measuring your probe signal before entering Run mode Special Considerations continued on other side Special Considerations Continued If the dextrose concentration in your sample exceeds the sucrose concentration the sub
28. a NULL argument in which case depending on the command a default parameter is supplied The 2700 responds to a legal command with a status acknowledgment A or an error code 1 2 6 7 8 or 9 The interpretations of the status characters are discussed under Communications Commands Remote Control Commands in Section 9 3 9 3 9 4 Error Codes Code 1 a 2700 is not in remote control mode This condition generally indicates that you invoked the TN1 command enter Run Mode remotely when the 2700 was not in remote mode You must first successfully invoke TR1 turn on remote control mode You may need to check 2700 status to determine the exact meaning of the error See Communications Commands Section 9 3 for more information b 2700 is in remote control mode but not in Run Mode This condition generally indicates that you requested that the 2700 Sample or Calibrate when the 2700 was not in Run Mode You must first successfully invoke TN1 command enter Run Mode remotely Code 2 2700 is busy in RUN mode Code 6 assignment of station is out of range s lt 1 or s 5 Code 7 purge time of zero is assigned when Station 5 s 5 is assigned monitor accessory Code 8 zero turntable position number is assigned t n 0 or default t n 0 Code 9 a sample or calibration results were not found when requested b 2700 is not in RUN mode c 2700 is in halt condition A halt condition can be caused by a cal error basel
29. and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water or 2357 buffer The sample must be dextrose free or at least contain levels low enough not to interfere with the sucrose reading Since the sucrose membrane contains glucose oxidase dextrose will produce a probe signal See Section 4 16 to measure dextrose and sucrose simultaneously 4 7 4 7 Lactose This is a direct reading of lactose in solution at the enzyme sensor The enzyme Galactose oxidase is immobilized in the enzyme membrane Lactose O io H O Galactose Dialdehyde Derivative System Buffer YSI 2705 Calibrator Std YSI 2783 5 00 g L Lactose Linearity Std YSI 2784 25 0 g L Lactose Black Probe YSI 2702 Galactose Oxidase Membrane White Probe n a Sample Size 25 uL Cal Station Station 1 Cal Well Black Probe Parameters Chemistry Lactose Unit of Conc g L Cal value 5 00 End Point 30 sec f you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations Ifsample dilution is required use reagent water The sample should be galactose free Galactose and other galactosides such as raffinose and stachyose are substrates for galactose oxidase They m
30. are recorded along with information from daily operational checks and other relevant information see Section 3 3 for more information MEMBRANE Figure 2 5 Enzyme Membrane Installation 2 7 2 5 2 8 Power Up Procedures BEFORE YOU PLUG IN THE POWER CORD inspect the Line voltage selector on the back of the instrument See Figure 2 6 Be certain the correct voltage is selected The arrowhead on the power selector must be pointing to the small rectangle on the housing as shown wv o 4 QU 110 1207 SELECTED 2 Figure 2 6 Line Voltage Selector 110 120V Position If the voltage selection is incorrect review Section 7 9 for correct voltage selection and fuse requirements or contact YSI Customer Service or your Dealer Representative If the voltage selection is correct plug in the unit using the power cord packaged with the instrument Set the power switch to ON 0 off and I on Correct Power up operation is confirmed by observing either of the following displays Main Menu Display Default Parameters Display Please select instrument mode Warning instrument parameters have RUN STANDBY MENU been set to default Hit key to set up The instrument will normally arrive with parameters including date and time set from the factory However if the backup rechargeable batteries have run down you will see the Default Parameters message This message will also appear if you ever ne
31. bar jumps or is set to maximum Next press 0 to return to the Select service menu level Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable When all adjustments are complete press MENU to return to Main Menu level Please select instrument mode RUN STANDBY MENU Printer Paper Installation Open the paper cover on top of the instrument Insert the loose end of the paper into the slot on the printer The outermost side of the paper on the roll should be facing down Press PAPER to advance the paper through the printer 2 11 2 8 Instrument Parameter Programming The 2700 SELECT setup is menu driven Once set up the system parameters are maintained in memory In the event of power loss the 2700 SELECT has a battery backup to maintain its memory Follow the procedure below to program your instrument parameters IMPORTANT You move through the 2700 SELECT menus by selecting options on the display Refer to Figure 5 1 for an overview of the menu structure You can press the MENU key to get back to the Main Menu and the 0 key to back up to the previous display However it may be necessary to confirm a response by pressing ENTER before continuing to use the MENU or 0 keys The Main Menu display is shown below Please select instrument mode RUN STANDBY MENU From the Main Menu press MENU The following display will appear Select instrument function 1 Service 2 Setup 3 Diagnostic
32. carton preferably the original and surround the product completely with packing material Insure for the replacement value of the product Cleaning Certificate Organization Department Address City State Zip Country Phone Model No of Device Lot Number Contaminant if known Cleaning Agent s used Radioactive Decontamination Certified Answer only if there has been radioactive exposure Yes No Cleaning Certified By F 4 Appendix G Line Power Cord and Plug Wiring Make sure that the cord and plug are appropriate for the power output you intend to use Make sure you use a plug to BS1363 and ASTA approval Use Fuse 3A to BS1362 and ASTA approval AC EARTH GREEN YELLOW LINE BROWN Green and yellow wire to E Earth BROWN wire to BROWN or L live BLUE wire to BLUE or N neutral NEUTRAL BLUE f fitti the t f th ket Before refitting the top cover o e plug or socket CEE TYPE 27 make sure that the cable clamp is securely holding the outer sheath of the cable IEC TYPE 320 240 VAC AC GROUND GREEN AC EARTH GREEN LINE BLACK LINE BLACK NEUTRAL WHITE NEUTRAL WHITE 110 VAC EC TYPE 520 NEMA TYPE 5 15P AC EARTH NEUTRAL BLUE GREEN YELLOW AC GROUND LINE BROW GREEN YELLOW LINE BROWN 220 VAC DIN TYPE 49 450 NEUTRAL BLUE CEE TYPE 7 7 SCHUKO CEE TYPE 27 IEC TYPE 320 Figure G 1 Line Power
33. communicates in ASCII over this interface The protocol used by the 2700 has been designed to be compatible with most computer database applications and most computer interface protocols The 2700 SELECT can be configured to communicate in Result Reporting mode or Remote Control mode The communications module also provides networking capability You may configure a 2700 SELECT to operate in either multidrop or non multidrop environments Multidrop refers to networking several 2700s and or other controllers to a host while non multidrop point to point refers to a single 2700 connected to a host controller The non multidrop configurations of software versions higher than 2 40 maintain compatibility with the earlier software version 2 03 The command format and communication protocols are identical for software versions 2 03 and up In sections below you will learn about communications handshaking software setup command structure communication modes and data base format Note The term 2700 SELECT has been shortened to 2700 in many sentences and figures below They are synonymous Communications Protocol The 2700 communicates with a host computer through its RS 232 serial port acting as a DTE The handshaking can be hardware or software configured The method is user selectable Figure 9 1 shows the RS 232 signal descriptions and directions with respect to the 2700 Pin Number Signal Name 2 00 Direction Signal Function Protecti
34. explore each menu parameter Access each parameter by pressing the number Press ENTER to confirm the default setting and return to the Autocal setup See Section 5 3 for more information WARNING When using autocalibration parameters other than the default values described in this manual you may compromise precision and or accuracy It is your responsibility as a user to verify performance through appropriate quality assurance testing Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error When you have completed this level press 0 to return to Run mode setup RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor Press 0 again to return to Select setup Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 4 for Report Select sample report format Brief 1 None 2 Brief 3 Detail The default setting is brief report Press ENTER to choose brief Sample report then press ENTER again to choose brief Cal report See Appendix D for example printouts of each type of report Select cal report format Brief 1 None 2 Brief 3 Detail The Select setup menu is again displayed Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 5 for PrntSetup Printing instrument setup Please wait The instrument will print the setup information The information is an itemized list of the key parameters tha
35. for SampleProtocol The display will read Sampling protocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 1 for SipperHeight This specifies to what vertical position the Sipper descends when sampling at the Manual Station 3 For example with long test tubes and the fluid level near the bottom of the test tube use Low setting Select manual sipper height Medium 1 Low 2 Medium 3 High Press ENTER to confirm and return to Sampling protocol setup Sampling protocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 2 for Replicates The default setting is No The display prompts reflect messages for single sampling and single calibrations If Yes is confirmed you will be prompted at the display each time you press SAMPLE or CALIBRATE to enter how many times the sample or cal solution should be run for that one command The number that you assign to Replicates must be between 1 and 999 samples or calibrations During RUN Mode you may cancel the assigned number of replicate cycles by pressing CANCEL As an example Replicates allows you to configure your system to run a sample in the Test Tube Holder unattended in duplicate or triplicate Replicates also allows you to check calibration stability by assigning some number of consecutive calibrations to be run without requiring repeated key commands of CALIBRATE 5 11 Prompt replicate cycle No 1 No 2 Yes Pre
36. for Service then press 2 for Buffer to initiate a 15 second run of the Buffer pump Make certain that all air is out of the buffer tubing Buffer should be exiting the Sipper inside the Sample Chamber and overflowing to waste You may need to initiate a second or third run of the buffer pump to complete the priming process Remember The fluid level sensor cables should not contact the instrument housing See Figure 2 3 for proper cable routing Check the Printer Paper Open the Paper Cover on the top of the instrument and check to see that there is sufficient paper Replace the paper roll as necessary See Appendix B for replacement part number Use the PAPER key to advance paper into the printer Check for Leaks Examine the tubing for leaks Also check for large air bubbles in the tubing These are caused either by loose connections or worn pump tubing Refer to Section 7 6 if tubing replacement is needed 7 2 Clean up Spills Spills should be cleaned up promptly to prevent biohazard build up and corrosion Clean any spills of biological material from the sample area If there is any evidence of salt build up around the base of the sample chamber follow the procedure in Section 7 3 to disassemble the chamber and clean the base Check that the drain tube fits tightly over the stainless steel fitting at the bottom of the chamber If loose replace the tubing See Appendix B for the part number of the 2700 SELECT preventive maintenanc
37. for any untransmitted result in the data base Remote Control Mode Remote Control Mode is designed primarily for industrial and research applications where process control may be an important consideration In Remote Control mode the instrument is in RUN Mode and under control of the host computer The instrument keypad is disabled thus the operator cannot run a sample or calibration locally at the 2700 The host tells the 2700 to run a sample or a calibration The samples may be assigned to run at Station 1 Cal Well Station 2 Test Tube Holder Station 4 Turntable or Station 5 Monitor Accessory See page 9 14 for more information Calibrations are typically assigned to run at Station 1 but may be assigned to Station 2 or even assigned to Stations 1 and 2 together in dual chemistry applications The remote host cannot change calibration station assignments they must be made locally at the 2700 SELECT 9 5 9 3 Communications Commands Data Transmission Records The data report format from 2700 to HOST is transmitted as shown in the example below Information includes time date temperature sample chamber node address re multidrop mode instrument identification ID sample identification number chemistry assigned to probe value sample or calibration reading unit of concentration nA current for calibrations error codes Notes A in the ID column specifies cal
38. for turntable unless the YSI 2710 Turntable has been installed Refer to the YSI 2710 Turntable Operation Manual for setup operation and maintenance of the turntable Press 0 to return to RUN mode setup RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor Press 2 for AutoStandby to define the number of hours the unit will continue to update calibration and be ready to sample The default setting is 2 If you press 0 and confirm with ENTER AutoStandby is disabled The instrument then remains sample ready indefinitely You may enter any number between 0 and 999 Enter autostandby time in hour 0 to disable gt 2 Press ENTER to confirm and return to RUN mode setup RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor Press 3 for AutoCal to define parameters that initiate autocalibrations The five categories are shown in the display below The default settings that initiate calibration are 1 temperature drift of more than 1 C since last cal 2 after 15 minutes without cal update 3 after 5 samples run without a cal update 4 after a probe current shift of 2 or greater compared to the last cal and 5 after certain errors are detected eg baseline instability Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error You may alter any of these parameters to suit your application however you may compromise precision and or accuracy when doing so YSI s stated speci
39. hydrolyze the starch and measure the total dextrose after hydrolysis Subtract the free dextrose from the total dextrose and multiply by 0 9 to account for water of hydrolysis 4 10 Choline This is a direct reading of choline in solution at the enzyme sensor The enzyme Choline Oxidase is immobilized in the YSI Choline Membrane Choline 20 2 Betaine Chol Oxidase System Buffer YSI 2357 Calibrator Std YSI 2772 Linearity Std YSI 2773 Black Probe YSI 2771 Choline Oxidase Membrane White Probe n a Sample Size 25 uL Cal Station Station 1 Cal Well Black Probe Parameters Chemistry Choline Unit of Conc mg L Cal value 175 End Point 30 sec f you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations lfsample dilution is required use reagent water or 2357 Buffer Although the YSI 2772 Choline calibrator solution is prepared using choline bitartrate the concentration is expressed as mg L of choline cation If you prefer to express the sample as a salt of choline you must enter an adjusted calibration value when configuring your instrument measurement parameters For example to express results as a choline hydroxide value program the calibrator value as 204 mg L when using the YSI 2772 Choline Standard calibrator solution Your results wi
40. if concentration unit conversion is required For applications requiring linearity performance to 25 0 g L YSI 2777 25 0 g L dextrose may be used as a linearity standard provided the sample size is 10 uL Tf you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Special Considerations lfsample dilution is required use reagent water or 2357 buffer Ifa solution must be prepared from solid dextrose use the following diluent and allow about 15 minutes before measuring the sample This is required for dextrose which must equilibrate alpha and beta anomers mutarotational equilibrium If your reading is lower than expected you may need to wait slightly longer for equilibration Diluent 40 g L NaH PO 10 g L Na HPO Reagent water Both heat and the presence of phosphate accelerate mutarotational equilibration 4 5 4 5 4 6 L Lactate This is a direct reading of L Lactate L Lactic Acid in solution at the enzyme sensor The enzyme L Lactate Oxidase is immobilized in the YSI L Lactate Membrane L Lactate gt L Lac Oxidase System Buffer Calibrator Std Linearity Std Black Probe White Probe Sample Size Cal Station Black Probe Parameters Chemistry Unit of Conc Cal value End Point H O Pyruvate YSI 2357 YSI 2776 0 50 g L L Lactate YSI 1530 2 67 g L L Lactate YSI 2329 L Lactate Membrane n a 25 uL Station 1
41. must be reconstituted as instructed on the bottle Add more reagent water until the total volume of solution is between 900 and 1000 mL Stir as necessary until the buffer chemicals have completely dissolved Disconnect the electrical lead from the level sensor and remove the bottle lid IMPORTANT When adding fresh buffer to the Buffer Supply Bottle or when installing a new bottle of Calibrator Solution make every effort to avoid contamination of the lid and level sensor assemblies Pour the prepared buffer into the supply bottle replace the lid and reconnect the lead Install the calibrator solution One or more of the following YSI calibration standards will be provided in your starter supplies YSI 2776 Dextrose and L Lactate YSI 2780 Sucrose YSI 2783 Lactose YSI 2772 Choline YSI 2755 L Glutamate YSI 2736 L Glutamine YSI calibrators may be purchased separately Y Unplug the electrical lead from the level sensor in the lid of the empty calibrator bottle and remove the lid Mark the date of installation on the new bottle of YSI calibrator solution Y Y Screw the lid and level sensor assembly onto the new bottle and place it in the instrument compartment Reconnect the electrical lead The YSI 2736 Glutamine Calibration Standard must be reconstituted as instructed on the bottle It is recommended that a sample be poured into a test tube and installed at station 2 for calibration Refriger
42. not given or NULL the default value will be used When the Clear Database Memory RZ command is invoked all of the sample results maintained by the 2700 database are cleared It is useful when the remote host is first switched to Remote Control Mode and wants to forget all previous samples that have been taken by an operator Host to 2700 RZ Clear 2700 database memory 2700 to Host A Acknowledge Result data cleared 1 2700 is not in Remote Control Mode When you invoke the RM Report Parameters command the instrument reports to you the current system time date and measurement parameters For ease of deciphering this information the report format is almost identical to that of the sample and calibration reports The only difference is that the Error Field beginning at column 62 contains additional information about the calibration parameters Host to 2700 RM Report instrument parameters 2700 to Host A Acknowledge 1 2700 is not in Remote Control Mode Figure 9 6 shows you the report format The first line is basically a ruler The second and third lines identify each field by an upper case letter A B C etc The number of characters in each field is the field width 123456789 123456789 123456789 123456789 123456789 123456789 123456789 12345 AA AA AA BB BB BB CCCCC DDD EEEEEEEEE FFFF GGGGG GG HHHHHHHH II J lt cr gt lt lf gt AA AA AA BB BB BB CCCCC DDD EEEEEEEEE LLLLL LL MMMMMMMM NN lt cr gt lt lf gt
43. rinse the tubing and steel tubes with water Remove the Cal Cap Assembly flush one time with air and wipe the cal cap and steel tubes with a clean laboratory tissue Install a new bottle of calibration standard and mark the installation date on the bottle as described in section 2 3 Reagent Preparation From the Service Menu initiate 3 Cal flushes to prime the Calibration Pumping System You are now ready to re enter Run Mode 7 3 7 3 7 4 Sample Chamber Cleaning It is necessary to periodically clean both the sample chamber and the base plate it sets on Move the Sipper to the manual sample position From the Main Menu press MENU Next press 3 for Diagnostic then press 1 for Motor then press 1 for Sipper motor Press 1 for Up to raise the Sipper from the Sample Chamber then press 4 for Clockwise to move the Sipper over to the Manual Sample position Note You must press 4 Clockwise twice to reach the manual position See the menu displays below to better understand this sequence of events Please select instrument mode RUN STANDBY MENU Select instrument function 1 Service 2 Setup 3 Diagnostic Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Motor diagnostic 1 Sipper motor 2 Stir motor 3 Turntable Sipper diagnostic 1 Up 2 Down 3 Home 4 Clockwise 5 Counterclock 6 Exercise Unscrew and remove all three probes from the sample chamber If your membranes are in good condition that is i
44. the brief report version of sample and calibration results As recommended for Initial Setup you will use all default measurement parameters In Section 2 8 you became familiar with the 6 categories in the Setup Menu and you were last instructed to press MENU to display Main Menu This is the level in the menu where we want to start If you have entered Run Mode or Standby Mode see page 3 1 for instructions to exit either mode At this time you may want to refer to Figure 5 2 Menu Flow Chart Main Menu Please select instrument mode RUN STANDBY MENU Press MENU Select instrument function 1 Service 2 Setup 3 Diagnostic Press 2 for Setup Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default 2 15 Press 1 for General General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 1 for Date Time Use the number keys to change entries Press ENTER to confirm each entry Enter date and time as required Year gt 98 Enter year Enter date and time as required Month gt 2 Enter month Enter date and time as required Date gt 14 Enter day Enter date and time as required Hour gt 17 Enter hour Enter date and time as required Minute gt 22 Enter minute Select date format MM DD YY 1 MM DD YY 2 DD MM YY Select and or confirm the format you desire for printed dates month day year day month year
45. the product returned transportation prepaid Repaired or replaced products are warranted for the balance of the original warranty period or at least 90 days from date of repair or replacement Limitation of Warranty This Warranty does not apply to any YSI product damage or failure caused by i failure to install operate or use the product in accordance with YSI s written instructions ii abuse or misuse of the product iii failure to maintain the product in accordance with YSI s written instructions or standard industry procedure iv any improper repairs to the product v use by you of defective or improper components or parts in servicing or repairing the product or vi modification of the product in any way not expressly authorized by YSI THIS WARRANTY IS IN LIEU OF ALL OTHER WARRANTIES EXPRESSED OR IMPLIED INCLUDING ANY WARRANTY OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE YSI s LIABILITY UNDER THIS WARRANTY IS LIMITED TO REPAIR OR REPLACEMENT OF THE PRODUCT AND THIS SHALL BE YOUR SOLE AND EXCLUSIVE REMEDY FOR ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY IN NO EVENT SHALL YSI BE LIABLE FOR ANY SPECIAL INDIRECT INCIDENTAL OR CONSEQUENTIAL DAMAGES RESULTING FROM ANY DEFECTIVE PRODUCT COVERED BY THIS WARRANTY YSI Factory Service Centers United States YSI Incorporated Repair Center 1725 Brannum Lane Yellow Springs OH e 45387 USA Phone 937 767 7241 Fax 937 767 9353 Europe YSI LT
46. to 30000 Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Press 4 for Cal shift Enter allowable autocal shift in 0 to disable gt 2 Confirm value shown or enter and confirm any number from 0 to 100 Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Press 5 for Sample error Autocal on sample error Yes 1 No 2 Yes Press ENTER to confirm performing an Autocal whenever a sample error is detected or press 1 for No then ENTER if you do NOT want the 2700 to perform an Autocal after detecting an error Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Press 0 to return to RUN mode setup RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor You have now completed Run mode setup Press 0 to return to Select setup menu level Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 4 for Report The display will read Select sample report format Brief 1 None 2 Brief 3 Detail Press ENTER to confirm sample report format and move to cal report format Select cal report format Brief 1 None 2 Brief 3 Detail Report Format refers to the information that will be printed each time the 2700 SELECT runs a sample or a calibration The default settings are Brief Detail report formats are typically used when you want to study probe currents and temperature c
47. you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations Ifsample dilution is required use reagent water The sample should be lactose free Lactose and other galactosides such as raffinose and stachyose are substrates for galactose oxidase They may interfere by producing artificially high galactose readings The enzyme membrane integrity test involving the use of YSI 2363 Potassium Ferrocyanide solution is not informative since ferrocyanide exists in the 2705 system buffer 4 12 Hydrogen Peroxide This is a direct electrochemical reading of hydrogen peroxide at the enzyme probe A structure similar in appearance to an enzyme membrane is used at the probe surface This membrane contains immobilized nonenzymatic protein to produce diffusion properties similar to those exhibited by YSI Enzyme Membranes The electrochemical reaction which is common to all YSI Enzyme Sensors is the following Platinum anode 2H O 2e System Buffer YSI 2357 see note below regarding catalase Calibrator Std n a Linearity Std n a Black Probe YSI 2701 Blank Membrane White Probe n a Sample Size 25 uL Cal Station Black Probe Parameters Station 42 Test Tube Holder Chemistry Peroxide Unit of Conc mg L Cal value user s choice recommend about 30 mg L End P
48. 0 SELECT will do the rest The Sipper moves to the sample test tube and immerses itself about 3 millimeters 1 8 inch below the surface of the fluid TEST TUB HOLDER f N y i I STATION N S PANU Figure 3 2 Sampling Stations Figure 3 3 The Test Tube Holder Pivoted Out The Sipper Pump Piston retracts and draws in 25 microliters of sample The Sipper moves back to the Sample Chamber the Sipper Pump Piston extends and the sample is dispensed In less than a minute the analyte values for the sample are displayed and printed An example of the display format is shown below Notice that the results are shown in line 2 while the Ready to sample message for the next analysis is in line 1 Ready to sample at Station 2 B LAC 0 00 g L W GLU 8 98 g L The printer format can be configured to express sample ID date time temperature sample size instrument serial number probe currents and more See Appendix D for example print outs For the purposes of learning basic operation compare the instrument value with the labeled value of the YSI standard The linearity standard should be within 5 of the label value For example if you calibrated the 2700 SELECT at 2 50 g L dextrose using YSI 2776 as the calibrator solution YSI 1531 9 00 g L should read between 8 55 g L and 9 45 g L dext
49. 2 4 2 5 2 6 227 2 8 2 10 3 1 3 4 3 4 3 6 5 1 5 2 5 17 6 1 6 3 6 4 6 5 6 7 7 5 7 7 7 8 7 9 7 10 7 11 7 12 7 12 7 13 9 1 9 2 9 2 9 7 9 8 9 15 9 16 1 1 1 Introduction Description The YSI Model 2700 SELECT Biochemistry Analyzer is a laboratory instrument intended for use in research food processing and bioprocessing applications THE MODEL 2700 SELECT IS NOT FOR HUMAN MEDICAL DIAGNOSTIC USE OR FOR HUMAN PERFORMANCE EVALUATION The Model 2700 SELECT offers precision and specificity comparable to more time consuming and rigorous methods but with greater speed convenience and sensitivity Many of the conditions which interfere with refractometers density meters and manual methods are of little concern when using the Model 2700 SELECT Measurements are virtually unaffected by sample color turbidity density viscosity pH volatility specific gravity temperature index of refraction optical activity or the presence of proteins or other biochemicals The Model 2700 SELECT can be set up to measure a number of different analytes but switching between them may entail some time and effort Configured as a single channel unit the Model 2700 SELECT provides quick measurements of any one of the following analytes e D Glucose Dextrose Lactate e Sucrose e Lactose e Ethanol e L Glutamate e Choline e L Glutamine e Methanol e Galactose e Hydrogen Peroxide Configured as a du
50. 23456789 123456789 Interpretation General a radix 0 for 1 for date format 0 for mm dd yy 1 for dd mm yy bottle level sensor flag 0 for off 1 for on sample size 5 to 65uL sample station 1 to 5 calibration method 0 for single 1 for dual Autocal autocal on chemistry error flag 0 for off 1 for on temperature shift between autocals time interval between autocals 0 to 65535 minutes number of samples between autocals 0 to 30000 cal shift allowed between cals RunMode autostandby time 0 to 30000 hours default starting turntable position 0 to 255 default number of turntable test tubes loaded 0 to 255 turntable fluid detection flag 0 for off 1 for on Monitor p monitor station number 1 to 5 monitor time interval 0 to 65535 minutes monitor precal time 0 to 65535 minutes monitor postcal time 0 to 65535 minutes external pump purge time 0 to 65535 minutes Figure 9 7 RI Command Report Format Additional remote control commands related to changing the external monitoring parameters and aborting a turntable run are described below Monitor Parameter Modification Commands MP MT MR MO MS You may use the commands described below to change monitor setup parameters remotely MPt change external pump purge time to t where t is in seconds MTt change monitor time interval to t where t is in minutes MRt change precal time interval to t where t is in minutes MOt change postcal time inte
51. 56789 03 01 B L Lactate 0 82 g L W Dextrose 5 31 g L Wed 02 25 98 13 22 56 Calibration Report Detail CALIBRATION REPORT B L Lactate Unstable IB current 1 79 nA PL current 11 63 nA FB current 1 51 nA Base shift 2 65 Slope 1 42 nA min End Point 30 Sec Cal shift 3 64 W Dextrose 2 50 g L IB current 0 88 nA PL current 5 81 nA FB current 0 76 nA Base shift 1 95 Slope 0 09 nA min End Point 30 Sec Cal shift 0 10 Temperature 26 33 9C Sample size 25 uL Thu 02 26 98 1475510 YSI 2700D 98 01234 Calibration Report Brief CALIBRATION REPORT B L Lactate 16 68 nA W Dextrose 12 81 nA Mon 02 23 98 17 12 09 D 1 D 2 Appendix E Required Notice The Federal Communications Commission defines this product as a computing device and requires the following notice This equipment generates and uses radio frequency energy and if not installed and used properly may cause interference to radio and television reception It has been type tested and found to comply with the limits for a Class A or Class B computing device in accordance with the specification in Subpart J of Part 15 of FCC Rules which are designed to provide reasonable protection against such interference in a residential installation However there is no guarantee that interference will not occur in a particular installation If this equipment does cause interference to radio or television recep
52. Cal Well L Lactate g L 0 50 30 sec If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water or 2357 buffer D Lactate is not a substrate for L Lactate Oxidase Therefore the 2700 SELECT cannot directly measure D Lactate If you have a known racemic mixture of lactates the L Lactate value multiplied by 2 should give you the total lactate value 4 6 Sucrose This is a direct reading of sucrose in solution at the enzyme sensor Three enzymes are co immobilized in the YSI Sucrose Membrane Invertase Mutarotase and Glucose Oxidase Sucrose H O gt a D Glucose Fructose Invertase o D Glucose lt _ gt p D Glucose Mutarotase B D Glucose O Guoaae H O D Glucono Lactone Through this chain of reactions the moles of hydrogen peroxide liberated is directly proportional to the moles of sucrose System Buffer YSI 2357 Calibrator Std YSI 2780 5 00 g L Sucrose Linearity Std YSI 2778 25 0 g L Sucrose Black Probe YSI 2703 Sucrose Membrane White Probe n a Sample Size 25 uL Cal Station Station 1 Cal Well Black Probe Parameters Chemistry Sucrose Unit of Conc g L Cal value 5 00 End Point 30 sec If you have a dual channel unit either Black or White or both Black
53. Cal Well and descends Calibrator solution is aspirated then moved to the Sample Chamber and dispensed just as you would observe in a normal calibration cycle The display probe current should rise above baseline and within 20 to 30 seconds should level off at some nanoampere value indicative of the particular chemistry and aspiration volume you have setup 5 21 5 22 B LAC 11 77nA W DEX 23 17 nA 1 Flush 2 Calibrator 3 Sample Next press 1 for Flush to clear the calibrator solutions from the Sample Chamber The flush will take about 30 40 seconds If the baseline has not returned to levels you previously observed you may want to initiate a second flush cycle Baseline values are shown again below B LAC 0 55 nA W DEX 1 80 nA 1 Flush 2 Calibrator 3 Sample Press 3 for Sample The Sipper will move to the assigned sample station and aspirate sample then move to the Sample Chamber to dispense sample just as was observed in the calibrator cycle above Again you must initiate the flush cycle by pressing 1 for Flush Probe current values will vary based on concentrations used for sampling B LAC 91 83 nA W DEX 112 9 nA 1 Flush 2 Calibrator 3 Sample Press 0 to return to the five diagnostic selections Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 4 for I O From this menu level you can run diagnostic tests on the input output devices of the instrument I O diagnostic 1 Keypad 2 Disp
54. Cord and Plug Wiring G 1 Appendix H Cleaning Disinfecting and Decontamination Procedures Proper precautionary lab practices should be followed when handling biological hazards Suggested cleaning and disinfecting solutions include Isopropanol 70 Sodium hypochlorite 5000 ppm free available chlorine 1 10 solution of household bleach Decontamination Procedures Remove and discard all tubing New tubing is provided in preventive maintenance kit Empty waste bottle and wash with disinfecting agent Remove Sample Chamber Sipper Test Tube Holder and probes according to instructions Thoroughly clean with disinfecting agent then rinse with warm water Remove probes and discard membranes Clean probes with isopropanol only rinse with warm water Clean up all spills then reassemble Appendix I CE Compliance Important Notice regarding CE standards compliance 1 Users should not open the front access door during operation internal components may be susceptible to ESD electrostatic discharge Users are cautioned not to touch the sipper needle during operation as discussed in the users manual The sipper mechanism is equipped with contact sensing circuitry for the purposes of fluid detection and human safety This circuitry may be susceptible to ESD ESD to the sipper needle may cause instrument malfunction Users should note that RS232 communications may be susceptible to ESD Data corruption or serial
55. D Lynchford House Lynchford Lane Farnborough Hampshire GU14 GLT England Phone 441 252 514711 Fax 441 252 511855 Japan YSI Japan Ltd e 3Fl Sakura Building 5 6 13 e Shinjuku e Shinjuku ku Tokyo 160 Japan Phone 813 5360 3561 Fax 813 5360 3565 YSI Authorized Service Centers California Fisher Scientific ISD 2822 Walnut Avenue Suite E Tustin CA e 92681 e Phone 800 395 5442 Georgia Fisher Scientific ISD 2775 Horizon Ridge Court Suwanee GA 30174 Phone 800 395 5442 Illinois Fisher 1600 West Gleenlake Avenue Itasca IIl e 60143 Phone 800 395 5442 New Jersey Fisher Scientific ISD 52 Fadem Road Springfield NJ 07081 Phone 800 395 5442 Pennsylvania Fisher Scientific ISD 585 Alpa Drive Pittsburgh PA 15238 e Phone 800 395 5442 F 1 Cleaning Instructions NOTE Before they can be serviced equipment exposed to biological radioactive or toxic materials must be cleaned and disinfected Biological contamination is presumed for any instrument probe or other device that has been used with body fluids or tissues or with waste water Radioactive contamination is presumed for any instrument probe or other device that has been used near any radioactive source If an instrument probe or other part is returned or presented for service without a Cleaning Certificate and if in our opinion it represents a potential biological or radioactive hazard our service person
56. G Keep your hands clear of the sipper arm and sipper while the instrument is in operation Service the sipper only when the instrument is in the service mode not in the run mode or standby mode To make certain the Sipper Arm and attached Sipper are correctly aligned follow the instructions below Press 1 for Sipper The Sipper Arm will move to the home position The tip of the Sipper should be centered over the large hole on the top of the Sample Chamber see Figure 2 7 If necessary loosen the adjustment screw and position the Sipper The adjustment tool hex key is included in the preventive maintenance kit that is packed with your instrument Retighten the adjustment screw Adjust sipper then select 0 Exit 1 Lower sipper for fine alignment 2 9 2 10 Press 1 for Lower sipper for fine alignment The Sipper will move closer to the small opening in the stainless steel cone Fine align sipper then select 0 Exit 1 Test sipper position The tip of the Sipper should be exactly centered above this opening If necessary loosen the adjustment screw again and position the Sipper exactly Retighten the adjustment screw Figure 2 7 Sipper Adjustment Position Press 1 for Test Sipper position The Sipper will descend into the Sample Chamber The Sipper should not contact the stainless steel cone If the Sipper position is still not exactly right press 1 and readjust it once more as described a
57. HE THHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHE I O diagnostic 1 Keypad 2 Display 3 Printer Press 3 for Printer The printer prints the same 95 characters described above in the display test The printout uses 5 lines and takes about 10 seconds to complete The display momentarily flashes the message Printer test then returns to I O diagnostic menu I O diagnostic 1 Keypad 2 Display 3 Printer 5 23 5 24 Press 0 to return to the five diagnostic selections Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 5 for Sensor You may select one of three categories of sensors temperature fluid level or detection and optosensors for a variety of motion status signals Sensor diagnostic 1 Temperature 2 LevelSensor 3 OptoSensor Press 1 for Temperature The display shows the current temperature of buffer in the Sample Chamber The temperature display is live i e changes will be updated continuously Reading sample chamber temperature Please wait Sample chamber temperature 25 10 C Hit any key to exit Press any key to return to Sensor diagnostic menu Sensor diagnostic 1 Temperature 2 LevelSensor 3 OptoSensor Press 2 for LevelSensor You will observe the current status of each of the four level sensors shown below The Sipper will show fluid or air The Cal and Buffer will show OK or Empt
58. In maintenance kits service manuals and part lists the Sipper may be referred to as the Sipper Needle or Sipper Tube CA STATION KEYPAD BUFFER PL MP 2 AN EN D L CALIBRA BUFFER BOTTLE OR BOTTLE Figure 2 1 Inside Front View of the 2700 SELECT The Buffer Pump draws buffer from its bottle pumps it through the Sipper Pump body and the Sipper and flushes the Sample Chamber The Calibrator Pump draws the appropriate standard solution from the Calibrator Bottle and fills the Calibrator Well in the Sample Chamber The Sipper Pump retracts its piston to draw in standard from the Calibrator Well or sample from a tube or container It extends its piston to dispense standard or sample into the Sample Chamber The Sipper Arm and Sipper Assembly is raised or lowered by one motor and moved horizontally to its various positions by another motor The positions are Calibrator Well Station 1 Sample Chamber home Test Tube Holder Station Station 2 Manual Station Station 3 Turntable Station Station 4 and Monitor Station Station 5 The Sipper capacitively senses fluid to control immersion depth and detect errors The Stir Bar is a plastic encapsulated magnet It is activated by a motor housed below the Sample Chamber It provides thorough mixing inside the chamber The Buff
59. Lactone Glu Oxidase System Buffer YSI 2357 Calibrator Std YSI 2776 2 50 g L dextrose Linearity Std YSI 1531 9 00 g L dextrose Black Probe YSI 2365 Dextrose Membrane White Probe n a Sample Size 25 uL Cal Station Station 1 Call Well Black Probe Parameters Chemistry Dextrose Unit of Conc g L Cal value 2 50 g L End Point 30 sec For applications requiring linearity performance to 25 0 g L YSI 2777 25 0 g L dextrose may be used as a linearity standard provided the sample size is 10 uL f you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Special Considerations An amyloglucosidase enzyme such as Sigma A7420 works with many samples Solubilizing starch is key to obtaining reproducible results A variety of methods are in the literature YSI has documented a couple of methods in YSI Application notes Prepare a starch solution using the diluent described below add 1 to 2 mg amyloglucosidase per mL of starch solution Allow at least 20 minutes for the enzyme to react and the liberated dextrose to reach mutarotational equilibrium before measuring the sample If your reading is higher than expected you may need to wait slightly longer or re evaluate the possibility of free dextrose in the sample Diluent 40 g L NaH PO 10 g L Na HPO in reagent water If free dextrose is a concern first measure free dextrose Then externally
60. Parameter Within this menu level you will confirm or change the parameter settings that define the chemistry or chemistries of interest Sample Size 1 SampleSize The default setting is 25 uL The range of choices is 5 to 65 uL You may enter any integer value Remember however this is a nominal volume The instrument does not depend on an accurate absolute value but rather reproducible aspirations This allows the calibrator probe signal to be stored in memory and provide a reference value used to internally calculate sample readings For each chemistry described a sample size usually 25 uL will be recommended Some of the reasons for changing that sample size are listed below The calibrator probe signal plateau current might be less than 10 nA Refer to Appendix D You may want to increase the sample size to increase the probe signal Probe plateau current is high enough but you would like to improve the linear range For example you might decrease sample size from 25 uL to 12 uL Calibration current might drop from 20 nA to 10 nA but accuracy and precision at the upper end of your concentration range should improve You may want to measure low concentrations of a particular analyte For example you might prepare a 500 mg L dextrose calibrator for a particular application If the dextrose calibrator produces probe signal plateau currents significantly below 10 nA you could increase the sample size from 25 to 50 uL or mo
61. Replicates Sample ID Sip Height Autostandby IT StaxtPos TT in Run TT FluidDet MONITOR MonStation SamInterval PreCal PostCal Purge Time RS 232 Baud rate Data Stop Parity Handshake XON char XOFF char Ej CY Ob Address 38 GENERAL Radix mark evel sensor Cal Report SampleReport DateFormat ode Non Dextrose g L 2 50 30 Sec 1 dE 15 Min 5 Sam OFF OFF Medium 2 Hr ON Min Min Min Sec 9600 Seven bit One bit Even RTS CTS 17 19 multidrop ON Brief Brief MM DD YY Software revision 2 56 YSI 2700S Thu 02 26 98 98 01234 09 20 48 INSTRUMENT SETUP Sample size SamStation 4 Cal Method On BLACK PROBE Chemistry Unit Calibrator End Point CalStation WHITE PROBE Chemistry Unit Calibrator End Point CalStation AUTOCAL SampleError Temperature Time Sample Cal Shift RUN MODE Replicates Sample ID Sip Height Autostandby StartPos in Run FluidDet MONITOR onStation SamInterval PreCal PostCal Purge Time RS 232 Baud rate Data Stop Parity Handshake XON char XOFF char ode Non Address 38 GENERAL Radix mark Level sensor Cal Report SampleReport DateFormat Software revi YSI 2700D 9 Thu 02 26 98 25 uL 2 e station L Lactate
62. SI 2756 and YSI 2737 Black Membrane YSI 2735 White Membrane YSI 2754 Sample Size 20 uL Cal Station Two Station 1 glutamate 2 glutamine Black Probe Parameters White Probe Parameters Chemistry Glutamine Chemistry Glutamate Unit of Conc mmol L Unit of Conc mmol L Cal value 5 00 Cal value 5 00 End Point 30 sec End Point 30 sec Since glutamine calibrator is at the Test Tube Holder you must sample at the Manual Station 3 The Turntable position 4 and the Monitor Station 5 may also be used on units equipped with these accessories Special Consideration The ability to select sample size 5 to 65 uL may help you optimize your system especially if very high or very low concentrations of one or the other of these substrates is a problem The optimal probe current for a calibrator is about 10 nA See Menu Diagnostics Section 5 4 to learn more about measuring your probe signal before entering Run mode 5 Menu Selections 5 1 Introduction In this section you will learn about the specific commands required to move through the 2700 SELECT menu structure You should begin by studying Figure 5 1 below recognizing that the instrument has two operating modes and a menu mode Also refer to Figure 5 2 next page The operating modes are RUN Mode and STANDBY Mode In RUN Mode the instrument calibrates samples or simply remains ready to sample In STANDBY Mode the instrument remains powered to keep the probes polari
63. Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 2 for Pump to access the Sipper Pump Calibrator Pump and Buffer Pump Pump diagnostic 1 SipperPump 2 Buffer 3 Cal 4 ExtPump Press 1 for Sipper Pump to begin a sequence of five distinct movements of the plunger rod in the Sipper Pump Locate the Sipper Pump a clear plastic cylindrical body mounted between the two peristaltic pumps on the fluid wall See Figure 5 3 Focus on the metal plunger rod inside this plastic body Some movements will be brief so watch closely Sipper pump diagnostic 1 Home Press 1 for Home You will see a small movement of the plunger but you will hear a clicking sound that lasts 3 or 4 seconds as the pump motor oversteps to detect home 5 19 5 20 The display will now update to give two new selections as shown below Sipper pump diagnostic 1 Home 2 Aspirate separator 3 Exercise Press 2 for Aspirate separator A short retraction of the plunger should occur This simulates the aspiration of an air bubble to isolate sample from the buffer stream The display updates to read the following Sipper pump diagnostic 1 Home 2 Aspirate sample 3 Exercise Press 2 for Aspirate sample A longer retraction of the plunger should occur This simulates the aspiration of sample The display updates to read the following Sipper pump diagnostic 1 Home 2 Dispense sample 3 Exercise Press 2 for Dispense sample An ext
64. Size 2 CalMethod 3 Black 4 White If you have a dual channel unit press 4 for White and repeat the steps that you performed for the Black probe The default settings are Dextrose g L 2 50 30 seconds All of the setup options for the Black probe are also available for the White probe Select WHITE chemistry Dextrose 0 Backup 1 Next chemistry ENTER Accept Press ENTER to confirm Dextrose as the WHITE chemistry Select WHITE unit of measurement g L 1 mmol L 2 mg L 3 g L 4 w v Enter WHITE calibration value in g L gt 2 50 Enter WHITE end point in seconds gt 30 Press ENTER to confirm and return to Measurement parameter setup Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White You have now completed MeasParameter setup Press 0 to return to the Select setup level Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 3 for RunMode The display will read RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor In this menu you select parameters related to sampling protocol set parameters that control automatic switching to standby mode and select parameters that will trigger autocalibrations In Sampling Protocol you can select the sample station position activate replicate sampling and sample identification systems and set the position to which the Sipper descends when sampling at the Manual Station Press 1
65. With the entry of the date format you will return to the previous menu level General setup General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 2 for Contrast Adjust the display contrast 1 Raise contrast 2 Lower contrast 2 16 Use the appropriate number key to adjust the LC display contrast for comfortable viewing When finished press 0 to return to General setup General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 3 for RS 232 RS 232 setup 1 Baud 2 Data 3 Parity 4 Stop 5 Handshake 6 Configuration This menu is used to set communication parameters Press 0 to return to General setup or explore these parameters remembering to press ENTER to move through each choice and back to RS 232 setup General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 4 for Radix Select radix mark yet 2 a 3 on In some parts of the world a is preferred to express decimal numbers Example 2 00 2 00 Confirm your choice by pressing ENTER to return to the General setup General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 5 for Serial Enter instrument serial number 98 12345 The serial of your instrument is recorded on the serial plate lower rear of case You will find it helpful to record this number in memory It will be printed in th
66. ail FCN Test Damaged or old membrane Replace membrane 2 4 SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION Fail Linearity Test Probe assignment incorrect Make correct assignment 4 0 Damaged or old membrane Replace membrane 2 4 Contaminated or old calibration or linearity standard Repeat test with new standards 3 4 Assigned concentration range beyond practical limits Redefine measurement parameters Remake standards 4 3 Keypad Disabled Instrument in Remote Control Mode Switch communications mode or reset 9 3 Faulty keypad or cable Check keypad in I O diagnostics 5 4 Printer Does Not Advance Paper or roll jammed Open paper well and clear obstruction Reset instrument if necessary If printer still does not advance turn instrument off for 30 seconds then back on Sipper Does Not Enter Chamber Sipper misaligned Check alignment 2 6 9 Communications 9 1 This section describes the communications protocol between the 2700 SELECT and a host computer The 2700 SELECT has an RS 232 serial port When you install the appropriate hardware interface e g shielded NULL modem cable the 2700 SELECT
67. al baseline error is printed when the instrument cannot adequately flush the chamber Slope Slope of the plateau See Figure 6 3 The slope is reported in nanoamps per minute A newly installed membrane may have an elevated plateau slope An excessive slope can be an indicator of the presence of an interfering substance End Point is the time from dispensing the sample into the sample chamber until the instrument reads the probe signal The default value for most chemistry setups is 30 seconds The value that you have selected in Setup is displayed in the report Note This is not through put time but rather best thought of as reaction time or probe signal development time Cal shift Calibration Shift A calibration result is compared to the previous calibration result and the percent shift is reported The default setting is 2 That is if the shift is greater than 2 the 2700 SELECT performs another calibration Up to 5 successive calibration shifts are permitted before the Unable to calibrate error is displayed You may select Cal shift values that better suit your application See Section 5 3 for details Excessive calibration shifts may be caused by faulty membranes newly installed membranes or air in the sample chamber Temperature Sample Chamber Temperature The sample chamber temperature is measured during a calibration and a sample The results of a sample are temperature corrected The 2700 SELECT works at sample chamber temp
68. al channel unit the Model 2700 SELECT provides quick simultaneous measurements of combinations of the following analytes e Dextrose and L Lactate e Dextrose and Sucrose e Glutamate and L Glutamine YSI does not currently offer calibration standards for these analytes 1 1 1 2 Flexibility is an important feature of the product Within practical limits a large number of measurement parameters can be selected to fit your application In addition the default parameters of many system functions can be changed to fit your needs Selectable sample size allows you to extend the dynamic range of the analyzer and improves precision and accuracy The aspirated sample size can be specified from a minimum of 5 microliters to a maximum of 65 microliters The Model 2700 SELECT is equipped with automatic self calibration However within limits the frequency of autocalibration can be programmed to meet your needs If instrument calibration is desired upon command only the self calibration feature can be disabled and calibration cycles can be initiated manually via the keypad or commanded by the host computer via the RS 232 interface YSI provides a full line of reagents to support the most common chemistries YSI offers the Model 2710 Turntable for batch sampling of up to 24 tubes or vials The 2710 Turntable is controlled by the 2700 SELECT software via a control cable provided The results of batch sampling are displayed and printed by
69. arameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 2 for CalMethod The default setting is One station The Two Station option is required for some dual channel analyses for example simultaneous dextrose and sucrose determination Select calibration method One station 1 One station 2 Two stations After pressing ENTER to confirm One station you must specify at which location the calibration solution will be The default location is Station 1 Cal Well The choices are 1 and or 2 Number 2 is the Test Tube Holder location With Accessories such as the YSI 2710 Turntable and YSI 2730 Monitor and Control Accessory stations 4 and 5 are also options Enter Cal Station gt 1 NOTE If Two station is selected you will first be prompted to identify the Black probe cal station and then the White probe cal station Press ENTER to confirm and return to Measurement parameter setup Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 3 for Black At this level you will choose chemistry and other black probe measurement parameters Select BLACK chemistry L Lactate 0 Backup 1 Next chemistry ENTER Accept 5 10 Press 1 for Next chemistry to scroll through the choices in the menu The choices are L Lactate Sucrose Lactose Galactose Peroxide Ethanol Choline L Glutamate L Glutamine Methanol none and Dextrose The Black probe default chemistry is L Lact
70. ate Press ENTER to confirm and continue to the unit of measurement parameter The choices are grams liter g L millimoles liter mmol L milligram liter mg L which is often referred to as parts per million ppm and percent weight volume Select BLACK unit of measurement g L 1 mmol L 2 mg L 3 g L 4 w v To confirm press ENTER then choose the calibration value For L Lactate the default value is 0 50 g L If you change units default values automatically change to reflect the new units of measurement If you are not using a calibrator with the default value you must enter the new value after a change of units Use Appendix A to convert to the appropriate value Enter BLACK calibration value in g L gt 0 50 For some numeric entries you may need to lead with a 0 Follow the cursor For example if you had 13 89 mmol L Dextrose entered and you wanted to change to 1 80 g L the calibration value would read 02 50 with the cursor under the 0 You would then need to enter 1 80 as 01 80 To confirm press ENTER and then choose the end point time for the stabilized enzyme sensor reading The default is 30 seconds The range is 15 to 125 seconds Use the default setting unless a particular application instruction specifies another value Enter BLACK end point time in seconds gt 30 Press ENTER to confirm and return to the Measurement parameter setup Measurement parameter setup 1 Sample
71. ate the bulk solution when not in use IMPORTANT The level sensor cables should not touch the instrument housing It is best to keep the cable connector on the bottle lid pointed toward the front False messages concerning fluid levels may result if the bottles are not installed inside the fluid compartment and the cables routed as shown in Figure 2 3 Figure 2 3 Bottles and Level Sensor Cables 2 5 2 4 2 6 Enzyme Membrane Installation Each active probe installed in your instrument is fitted with a protective shipping membrane which must be removed and replaced with a new membrane from the starter supplies The Sample Chamber is color coded to assist you in membrane installation and setup The left side of the chamber has a black probe housing and the right side has a white probe housing Throughout this manual whenever we refer to the black or white probe the reference is to the black or the white side of the sample chamber The 2700 SELECT is shipped to you already configured as a Single Channel or a Dual Channel unit For the single channel configuration the white probe is a blank see Figure 2 4 Figure 2 4 Sample Chamber Sensor Configurations One or more packs of YSI Immobilized Enzyme Membranes is provided in the starter supplies Each pack contains four membranes Enzyme Membrane O rings are color coded for each type of chemistry For dual channel configurations it is importa
72. ay interfere by producing artificially high lactose readings The enzyme membrane integrity test involving the use of YSI 2363 Potassium Ferrocyanide solution is not informative since ferrocyanide exists in the 2705 system buffer 4 8 4 8 Ethanol This is a direct reading of Ethanol in solution at the enzyme sensor The enzyme Alcohol Oxidase is immobilized in the enzyme membrane Ethanol HO Acetaldehyde Alcohol Oxidase System Buffer YSI 2787 Calibrator Std YSI 2390 Solution A 2 00 g L Linearity Std YSI 2390 Solution B 3 20 g L Black Probe YSI 2786 Alcohol Oxidase Membrane White Probe n a Sample Size 10 uL for best linearity Cal Station Station 42 Test Tube Holder Black Probe Parameters Chemistry Ethanol Unit of Conc g L Cal value 2 00 g L End Point 45 sec If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry You may use Station 1 Cal Well but evaporation through plastic components may be a problem See the evaporation control information in special considerations Note See Appendix A if concentration unit conversion is required Special Considerations The linear range of ethanol is quite limited If you are concerned about linearity monitor the upper range of concentration on a regular basis You may benefit by preparing and using a calibrator with an ethanol concentration close to your samp
73. bove IT IS VERY IMPORTANT THAT THE SIPPER BE ACCURATELY ADJUSTED Select 1 to restart check sipper cycle 0 Exit 1 Home sipper position After adjustment is complete press 0 to exit and return to the Select Service menu level Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable 2 7 Press 2 for Buffer The Sipper will enter the Sample Chamber and the Buffer Pump will begin to prime the fluid system with buffer solution The fluid system is completely primed when buffer flows from the steel cone at the top of the sample chamber Press 2 again if necessary Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable Press 3 for Cal The Calibrator Pump will begin to pump calibrator through the calibrator line into the Calibrator Well in the Sample Chamber If necessary press 3 again until calibrator flows out of the tube in the cal well Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable Press 4 for Stir Speed The menu message shown below will appear The stir bar has two operating speeds normal speed at which the stir bar rotates smoothly in the chamber and accelerated speed at which the stir bar loses synchronization with the motor housed below and jumps This jumping action helps clear the Sample Chamber of air bubbles during a flush cycle Adjust until stir bar jumps 1 Increase speed 2 Decrease speed Adjust the speed until the stir
74. c 1 SipperPump 2 Buffer 3 Cal 4 ExtPump Press 3 for Calibrator to turn on the Calibrator Pump The pump runs until you press 3 a second time The display remains as shown below You should hear the pump and see fluid moving into the Cal Well of the Sample Chamber Block and then flowing out through the Waste Tube to the Waste Bottle Pump diagnostic 1 SipperPump 2 Buffer 3 Cal 4 ExtPump Press 0 to return to the five diagnostic selections NOTE Unless the 2730 Monitor and Control pump is installed 4 ExtPump will give no response Refer to the YSI 2730 Monitor and Control Accessory User s Manual for details of external pump operation Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 3 for Probe You will observe a brief display message as shown below which then changes to show probe current s You will observe only Black probe current for a single channel unit The probe activity is expressed in nA nanoamperes of electrical current A nanoampere is 10 amperes which is a very low level of electrical current Reading probe current Please wait B LAC 0 50 nA W DEX 1 76 nA 1 Flush 2 Calibrator 3 Sample NOTE If you assign none for a probe in the Setup measurement parameters menu or you have a single channel unit the display appears as shown below B LAC 0 50 nA W Not assigned 1 Flush 2 Calibrator 3 Sample Press 2 for Calibrator The Calibrator Pump turns on as the Sipper moves to the
75. c to display the five categories of diagnostic selections Select instrument function 1 Service 2 Setup 3 Diagnostic Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 1 for Motor to gain access to two motors the Sipper Arm assembly vertical and horizontal motions and the Stir Motor that turns the magnetic stir bar in the Sample Chamber Motor diagnostic 1 Sipper motor 2 Stir motor 3 Turntable Press 1 for Sipper motor to display the following motion selections Sipper diagnostic 1 Up 2 Down 3 Home 4 Clockwise 5 Counterclock 6 Exercise When you press one of the keys 1 5 a movement of the Sipper should be observed Press 1 and the Sipper moves up until the upper limit optosensor is detected Press 2 and the Sipper moves down into the Sample Chamber or to its limit if at another station CAUTION Before you initiate exercise in the horizontal plane remove the left side access plate to prevent damage if the sipper moves to the turntable or monitor position If you have a turntable or monitor installed properly the access plate will already be removed In the horizontal plane press 3 and the Sipper moves to the position directly over the Sample Chamber opening Press 4 and the Sipper moves horizontally clockwise one station for ex ample from home to Test Tube Holder Station Station 2 Press 5 and the Sipper moves horizontally counterclockwise one station for example from Manual Station
76. cations menu is a submenu of General setup Listed below are the default settings and options for each communications parameter mentioned above The default value is listed first The options are in parentheses Baud rate 9600 4800 2400 1200 600 300 Data Length 7 8 Parity Even odd low high none Stop Bit 1 2 Handshake RTS CTS XON XOFF None Configuration non multidrop multidrop Data and Command Format The 2700 behaves like a slave to the host computer It never talks to the host unless in response to a command from the host The 2700 will respond with an ASCII lt BEL followed by the display of an error code or for an illegal command A command is defined to be a string of printable upper case ASCII characters Blanks are ignored Command Grammar The command grammar is shown below Abbreviations include ESC escape cmd command addr address arg argument and cr carriage return lt ESC gt amp cmd arg cr non multidrop lt ESC gt lt addr gt cmd arg cr multidrop In this grammar addr gt is a single byte binary node address that you assign to the 2700 SELECT You make the assignment via the instrument keypad The 2700 SELECT only responds to commands with the addr gt field that matches the assigned address A command may require one or more arguments If multiple arguments are given they must be separated by a semicolon Two consecutive semicolons indicate
77. communications failure may occur If malfunction occurs users may restore proper operation by resetting communications parameters under the General RS 232 setup menu I 1 YSI incorporated 1725 Brannum Lane Yellow Springs Ohio 45387 USA 062521 937 767 7241 800 659 8895 Fax 937 767 8058 A23781L July 2000 Info ysi com www YSI com 2000 YSI Incorporated
78. confirm or change the date set date format adjust the display contrast define communication parameters select the radix to express decimal numbers confirm or enter the instrument serial number and select whether to deactivate the bottle level sensor system Press 1 for Date Time Use the number keys to change entries Press ENTER to confirm your selection You will see the display messages below leading you through the complete date entry sequence Enter date and time as required Year gt 98 Enter date and time as required Month gt 2 Enter date and time as required Date gt 14 Enter date and time as required Hour gt 17 Enter date and time as required Minute gt 22 Select and or confirm the format you desire for printed dates month day year or day month year Select date format MM DD YY 1 MM DD YY 2 DD MM YY When you press ENTER you will return to the General setup level General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 2 for Contrast Use the appropriate number key to adjust the LC display contrast for your comfort When finished press 0 to return to General setup level Adjust the display contrast 1 Raise contrast 2 Lower contrast General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 3 for RS232 Use this menu to set communication parameters if you are using the serial interface port See Section 9 for detail
79. d samples the cable may break To prevent unexpected down time replace this cable every 6 months Extra cables are supplied in the Preventive Maintenance Kit Pull the connector out of the printed circuit board on the Sipper Arm Remove the terminal end of the cable from under the Sipper mounting screw Install a new cable Refer to Section 2 6 to readjust the Sipper 7 9 7 9 Fuse Replacement It may be necessary to replace the fuses in the 2700 SELECT New fuses may be purchased from YSI or obtained from many local electrical component suppliers Be sure to obtain the correct fuse rating as indicated below UNPLUG THE INSTRUMENT FROM THE MAINS SUPPLY Using a small screw driver pry open the fuse holder on the power receptacle see Figure 7 4 Sal Oy ee 6 220 240V Selected FUSE 1 Dl Figure 7 5 Fuse Replacement Fuse Requirements Fuse Type 110 120 VAC Operation 1 Amp YSI 062443 Fast acting 3AG 250 volt 0 25D x 1 25L inches For Europe and UK use fuse type 220 240 VAC Operation 1 2 Amp YSI 019722 Anti surge T 250 volt 5mm x 20mm L IEC127 amp BS4265 Install new fuses in place of the old ones and return the fuse holder to the power receptacle Be certain the correct voltage is selected The arrowhead on the power selector must be pointing to the small rectangle on the housing as shown inset With the power switch in the off O position plug the power cord into the ins
80. d to be used only when the enzyme sensor stops operating normally and the above maintenance procedure is ineffective This is not a routine maintenance procedure 1 With the membrane removed immerse the sensor in a 14 solution of ammonia for 3 minutes 2 Immediately after soaking rinse the sensor with reagent grade water for 3 5 minutes It is important that all the residual ammonia is removed 3 Prepare a small amount of 0 5 sodium hypochlorite solution Immerse the sensor in the solution for 30 45 seconds Check periodically sensor should darken but do not allow it to become black 5 Remove the sensor and immediately rinse it with reagent water for at least 2 minutes 6 Install a new membrane and return the probe to service The procedure below is only recommended as a last resort for revitalizing a sensor after the above cleaning procedure has been unsuccessfully attempted WARNING The following procedure may cause permanent damage to the enzyme sensor 1 Using a clean new pencil eraser carefully rub the center electrode platinum of the sensor Do not rub the epoxy between the two electrodes or the outer silver electrode 2 remove any eraser dust or residual rinse sensor with 70 isopropyl alcohol and then reagent grade water Prepare a small amount of 0 5 sodium hypochlorite solution Immerse the sensor in the solution for about 45 seconds Remove the sensor and immediately rinse it with reagen
81. e detailed report format and will be very useful if technical assistance or repair is required Since YSI uses alpha characters in the serial we recommend that you use a space for these characters Example 98B12345AB can be entered as 98 12345 You will find the number in memory since it is entered prior to shipment and should be preserved by battery backup Press ENTER to confirm and return to General setup General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor Press 6 for LevelSensor Activate bottle level sensors Yes 1 No 2 Yes 2 17 2 18 Choosing Yes maintains level sensing in the Buffer Calibrator and Waste bottles Press ENTER to return to General setup General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor You have now completed General setup Press 0 to return to Select setup menu level Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 2 for MeasParameter Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 1 for SampleSize Enter sample size in microliter gt 25 The default setting is 25 microliter uL The options range from 5 to 65 uL If you enter a number outside of this range the display will show you the limits Press ENTER to confirm 25 uL and return to the Measurement parameter setup menu level Measurement parameter setup 1 SampleSize 2 CalM
82. e kit Daily Operational Checks To verify proper instrument performance perform the daily operational checks described in Section 3 3 Calibration Pumping System Maintenance Perform this procedure at least once a month to minimize the possibility of contamination The most convenient time to perform this maintenance is before installation of a new bottle of calibration standard at Station 1 Exit Run or Standby Mode to prevent autoflushing and autocalibrations This also allows you to access Service Menu from the keypad Press MENU then 1 for Service Prepare about 100 mL of a nominal 1 solution of hypochlorite 1 part commercial bleach with 3 parts deionized or distilled water and place this solution in a clean 250 mL bottle that has threads compatible with the Cal Cap Assembly at Station 1 Attach the Cal Cap Assembly tighten and shake gently to insure thorough washing of the inside of the cap assembly From the Service Menu initiate cal priming to flush hypochlorite solution through the pump tubing and steel tubes to the Sample Chamber Waste Bottle Repeat this flush cycle 3 times wait 10 minutes then flush one more time Remove and discard the hypochlorite solution then rinse the bottle thoroughly with water Next add deionized or distilled water to the bottle reattach the Cal Cap Assembly at Station 1 and shake gently to thoroughly rinse the inside of the cal cap From the Service Menu initiate 3 flushes to
83. eaction An enzyme is a protein molecule with great specificity that catalyzes the conversion of one or more substrates to one or more products In the YSI technology an oxidase enzyme is always involved since one product of an oxidase reaction is hydrogen peroxide Hydrogen peroxide is required to produce a meaningful signal current at the electrochemical probe A substrate like dextrose enters the sample chamber and is stirred and diluted The substrate then diffuses through a thin polycarbonate membrane material The rate of the chemical reaction shown below is limited primarily by diffusion This results in improved linearity calibration stability and freedom from enzyme inhibition errors 4 1 4 2 4 2 Once past the polycarbonate membrane the substrate encounters an extremely thin layer of the appropriate oxidase enzyme There the following reaction occurs Substrate gt H O Byproduct Although oxygen is consumed in this reaction the buffer is not seriously depleted of oxygen nor is the rate of enzyme reaction very sensitive to small changes in oxygen concentration Therefore it is not necessary to measure or control the oxygen content in the sample chamber Hydrogen peroxide diffuses toward the platinum anode in the probe assembly This gives rise to the probe signal current The platinum anode is protected from other oxidizable substances by a thin layer of cellulose acetate This makes the anode specific for hydrog
84. ed information on communications Below are the display messages showing default settings and your options Press ENTER to confirm your choices for each parameter If you are not using the serial port you need not worry about setting these parameters They will not interfere with other parameter settings RS 232 setup 1 Baud 2 Data 3 Parity 4 Stop 5 Handshake 6 Configuration Select baud rate 9600 1 9600 2 4800 3 2400 4 1200 5 600 6 300 Select number of data bits Seven bit 1 Seven bit 2 Eight bit Select parity Odd 1 None 2 Even 3 Odd 4 Low 5 High Select number of stop bits One bit 1 One bit 2 Two bits Select handshaking type RTS CTS 1 RTS CTS 2 XON XOFF 3 None If you choose XON XOFF you will go one menu level deeper to enter or confirm XON and XOFF values Enter XON character gt 17 Press ENTER to confirm Enter XOFF character gt 19 Press ENTER to confirm and return to RS 232 setup RS 232 setup 1 Baud 2 Data 3 Parity 4 Stop 5 Handshake 6 Configuration If you press 6 for Configuration you will see the following Select configuration Non multidrop 1 Non multidrop 2 Multidrop The configuration option allows operation in either a point to point environment or in a multidrop environment with an address ID assigned to your 2700 SELECT unit s The default configuration is Non multidrop If you press 2 for Multidrop you will see the following Enter node address gt 38
85. ed to change batteries in the future If you see the Default Parameter message press any key to access the Setup Menu then press MENU to return to Main Menu as displayed above Specific instructions to set date time and other parameters are described below If the Main Menu Display does not appear immediately or after you have tried the procedures above reset the instrument by pressing the reset switch on the back panel or by turning the power off waiting about 20 seconds then turning the power back on 2 6 Fluid System Priming Since it may take an hour or more to initially stabilize the probes when setting up for the first time now is a good time to prime the fluid system You have already reconstituted the appropriate buffer and transferred it to the Buffer Bottle You have also installed the appropriate calibrator solution and installed the appropriate Enzyme Membrane s in the Sample Chamber With the Main Menu displayed see below press MENU then press 1 for Service Please select instrument mode RUN STANDBY MENU Select instrument function 1 Service 2 Setup 3 Diagnostic Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable You are now ready to align the Sipper prime the fluid pumps and adjust the stirring Since the adjustments were made prior to shipment this procedure will likely be just a check IMPORTANT The front door must remain open to carry out this procedure WARNIN
86. embrane Kit Choline Oxidase Membrane Kit Standards for 2700 Select YSI 4 2726 2736 2737 2755 2756 2772 2773 2776 1530 1531 2778 2780 2783 2784 2790 Product Description Methanol Standard Kit 1 00 g L 2 50 g L L Glutamine Standard 5 00 mmol L L Glutamine Standard 8 00 mmol L L Glutamate Standard 5 00 mmol L L Glutamate Standard 10 0 mmol L Choline Standard 175 mg L Choline Standard 450 mg L Dextrose L Lactate Standard Dex 2 50 g L Lac 0 50 g L L Lactate Standard 30 0 mmol L 2 67 g L Dextrose Standard 9 00 g L Sucrose Standard 25 0 g L Sucrose Standard 5 00 g L Lactose Standard 5 00 g L Lactose Standard 25 0 g L Ethanol Standard Kit 2 00 g L 3 20 g L Quantity A kit A kit A kit A kit A kit A kit A kit A kit A kit A kit Quantity 180mL 120mL 250mL 156mL 250mL 125mL 250mL 125mL 250mL 125mL 125mL 125mL 250mL 250mL 125mL 180mL 120mL B 1 B 2 Other Available YSI Standards YSI 4 2327 2328 2356 2747 2748 2777 Product Description L Lactate 5 0 mmol L 0 45 g L L Lactate 15 0 mmol L 1 34 g L Glucose 500 mg dL 5 00 g L Dual Standard Glucose 1 80 g L L Lactate 0 45 g L Dual Standard Glucose 18 0 g L L Lactate 1 78 g L Dextrose L Lactate Standard Dex 25 0 g L Lac 2 50 g L General Reagents And Buffers YSI 4 2357 1579 2705 2787 2363 2392 Product Description Buffer Kit all membranes except 2702 2725
87. embrane O ring Color User Must Provide Potential Substrate Interference Hydrogen Peroxide Membrane Detection Range Typical Membrane Working Life Membrane O ring Color User Must Provide 2702 0 25 g L 10 days White Calibrator solution Lactose 2701 0 600 mg L 21 days Yellow Calibrator solution Catalase free Supply Buffer 1 7 1 5 How to Use this Manual If your instrument is configured for single channel chemistry this instruction manual contains more information than you require but the measurement technology and the principles and techniques of operation remain the same This manual is organized in such a way as to give you the quickest possible start in operating the instrument However it cannot be stressed too strongly that informed and safe operation is more than just a matter of knowing which buttons to push An understanding of the principles of operation and potential chemical interferences is necessary for the wisest interpretation of results Thorough precautions regarding the handling of biological samples are also essential for the safety of operators The early parts of this manual will teach you how to get the instrument running Additional topics are included to help you understand the science it employs how to use it most effectively and safely and how to keep it operating correctly 2 Initial Setup 2 1 We recommend that your dealer representative or YSI regional representat
88. en peroxide Low molecular weight reducing compounds can interfere Typical interferences include phenols hydrazine hydrogen sulfide and mercaptans Sample Preparation A variety of sample types can be analyzed with the 2700 SELECT Generally the only sample preparation that may be required is dilution of the sample to bring the substrate concentration within the linear range of the instrument see Section 1 4 for the working range of each chemistry Neither color nor turbidity interferes with measurements Small particles do not affect the reaction in the sample chamber that houses the probes but samples with particles large enough to clog the sipper should be avoided Further information and specific procedures for determining the concentration of chemical analytes in many products or specimens are available in YSI Application Notes If you would like to discuss your application or inquire about other uses for the 2700 SELECT contact YSI Technical Support address and phone information on the back cover of this manual 4 3 Measurement Parameter Setup Information In order to configure your instrument to measure a particular chemistry analyte you need to Approximate the analyte concentration or range of concentrations to be measured Decide if you must dilute your sample and if required determine an appropriate dilution factor and diluent Decide what calibration value s is appropriate for the range of concentration
89. ension of the plunger should occur This simulates dispensing the sample into the Sample Chamber The display updates to read the following Sipper pump diagnostic 1 Home 2 Dispense separator 3 Exercise Press 2 for Dispense separator A short extension of the plunger should occur This simulates dispensing the air bubble into the Sample Chamber after the sample reading at the probe would have occurred The plunger is now back to home position Sipper pump diagnostic 1 Home 2 Aspirate separator 3 Exercise Press 3 for Exercise All of the plunger movements will occur in the sequence described above The complete retraction and extension represents one cycle The cycle number is displayed Exercising sipper pump Cycle 3 Hit any key to stop When you press a key to terminate the exercise mode the printer records the activity and number of cycles completed The display returns to Sipper pump diagnostic menu Sipper pump diagnostic 1 Home 2 Aspirate separator 3 Exercise Press 0 to return to Pump diagnostic menu Pump diagnostic 1 SipperPump 2 Buffer 3 Cal 4 ExtPump Press 2 for Buffer to turn on the Buffer Pump The pump runs until you press 2 a second time The display does not change messages during this activity You should hear the pump and see fluid moving through the Sample Chamber and exiting through the opening of the steel cone then through the Waste Tube to the Waste Bottle Pump diagnosti
90. entive Maintenance The YSI 2788 Preventive Maintenance Kit contains all supplies necessary to keep your 2700 SELECT operating properly Perform the Calibration Pumping System Maintenance detailed in Section 7 2 at least once every month Perform the maintenance procedures in Sections 7 3 to 7 11 every 6 months or as necessary Section 7 9 Fuse Replacement is not preventive maintenance and obviously requires attention only when fuses fail Section 7 5 Probe Cleaning is not recommended unless problems related to the enzyme sensor system are indicated Daily Maintenance Empty the Waste Bottle If the 2700 SELECT is used for medical research or biological testing dispose of the waste bottle contents in a manner suitable for biohazardous waste The reagents used in the 2700 SELECT are non toxic and unless otherwise specified consist of a phosphate salt buffer with small amounts of preservatives YSI 2705 phosphate buffer also contains Potassium Ferricy anide Refer to reagent bottle labels and Material Safety Data Sheets for more information Check the Calibrator Bottle If the fluid level is low or the bottle has been in the instrument longer than 30 days install a new bottle of calibrator solution Follow the instructions in Section 2 3 Reagent Preparation After installation prime the Calibrator Well From Main Menu press MENU See menu information displayed below Next press 1 for Service then press 3 for Cal to initiate a 10
91. er Aspirating sample The results are then displayed and printed as shown above Again compare the value reported by the instrument with the value on the label of the YSI 1531 Standard 9 00 g L dextrose Repeat calibration and sampling if the result is not within specified tolerance limits of 5 as described above If your reading is still outside this range refer to Linearity Test below Figure 3 4 The Manual Station 3 3 3 4 Standby Mode If no sample is processed for 2 hours the 2700 SELECT automatically exits RUN Mode and enters STANDBY Mode In STANDBY Mode the unit conserves reagents It does not self calibrate it only freshens the Sample Chamber with buffer using less than 1 milliliter every hour You can manually enter STANDBY Mode from either RUN Mode or the Main Menu by pressing the STANDBY key The 2 hour default for entering STANDBY Mode can be changed to better fit your application If you have not yet done so press MENU The following menu should appear Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 3 for RunMode Then press 2 for AutoStandby The following display will appear Enter autostandby time in hour 0 to disable gt 2 If you would like your 2700 SELECT never to leave Run Mode press 0 and it will continually self calibrate and maintain a sample ready status Otherwise enter the number of hours 0 to 65 535
92. er Waste and Calibrator Bottles are visible through the front door window for easy monitoring of fluid levels A stainless steel shaft projecting into each bottle terminates with a connector on the lid providing a signal used to halt operations when the Buffer or Calibrator Bottles are empty or when the Waste bottle is full The Sample Chamber is made of clear acrylic plastic White and black holders for the sensor probes are screwed to either side The immobilized enzyme membranes on the sensor probes are mounted on O rings which act as fluid seals on each side of the Sample Chamber A reference or auxiliary electrode is housed in the temperature probe and positioned at the back of the Sample Chamber It is held in place by a retainer that threads directly into the Sample Block A small black O ring slips over the temperature probe electrode to provide the seal The Calibrator Well is located behind and to the right of the Sample Chamber entry port The Test Tube Holder pivots out to allow insertion or removal of several common size test tubes The Display is a 2 line by 40 character liquid crystal display The Printer provides a hard copy record of sample results calibration currents and errors It uses 2 1 4 inch 56 mm wide thermal paper The Keypad is a 20 key membrane switch It has 0 9 numeric keys SPACE and BACK keys and 8 function keys The Reset Switch is located on the back of the instrument It is used to hard reset the operati
93. er RUN Mode by pressing RUN and you exit RUN Mode by pressing RUN You enter STANDBY Mode by pressing STANDBY and you exit STANDBY Mode by pressing STANDBY In each action above you must confirm your intention by pressing 2 or 1 for yes or no respectively then press ENTER to confirm your choice Run Mode To enter Run Mode follow the instructions below If required return to the Main Menu by pressing MENU or by exiting RUN or STANDBY Mode The display must read as follows Please select instrument mode RUN STANDBY MENU Press RUN to enter Run Mode The Buffer Pump will operate through two cycles and the instrument will initialize the baseline current which means it will ready itself to calibrate Two or more calibration cycles will be run automatically The Sipper moves out of the Sample Chamber and enters the Calibrator Well or other preset calibrator station Calibration standard is aspirated into the Sipper which then returns to the Sample Chamber and dispenses the standard After the measurement the Buffer Pump flushes the standard from the chamber The following display messages will appear during initialization Entering RUN mode please standby Initializing mechanism Entering RUN mode please standby Stabilizing baseline current Entering RUN mode please standby Stabilizing calibration NOTE In Run Mode the unit calibrates itself every 15 minutes or every 5 samples It will someti
94. eratures between 15 and 35 C The 2700 SELECT only measures and displays temperatures between 10 and 40 C If the temperature is outside this range a string of asterisks will be printed 8 2 Troubleshooting Chart SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION ERROR Baseline Pinched leaking or disconnected tube Fix or replace tubing 7 6 Sipper misaligned Check Sipper alignment 2 6 Buffer pump not operating properly Replace tubing 7 6 Stir bar not in chamber Disassemble chamber and reinstall stir bar 7 3 Newly installed enzyme membrane Enter probe diagnostics and check probe currents 2 6 Newly installed probe Enter probe diagnostics and check probe currents 2 6 Power disruption Enter probe diagnostics and check probe currents 2 6 Failing enzyme membrane Perform daily operational checks and replace membrane s if necessary 3 4 Enzyme Probe surface s fouled Clean probe surface s 7 5 Temperature Probe auxiliary electrode fouled Clean probe surface 7 5
95. essing RUN to exit RUN Mode or pressing STANDBY to exit STANDBY Mode You have limited access to Setup options by pressing MENU from the RUN or STANDBY modes This enables you to correct the time or date change report format or turn on a special feature without leaving the operating mode The only Setup selections not accessible from RUN or STANDBY mode are MeasParameter and Default both of which allow you to change parameters that could affect sample results leading to erroneous data For detail on menu selections see Section 5 For an overview see Figure 5 2 Menu Flow Chart Please select instrument mode RUNI STANDBY MENU i STANDBY STANDBY STANDBY STANDBY MODE RUN i CALIBRATIUN Figure 6 5 2700 SELECT Software Structure 7 Maintenance 7 1 Before performing maintenance on the 2700 SELECT always exit RUN or STANDBY Mode While in these modes the unit periodically calibrates or flushes Servicing the fluid system in either RUN or STANDBY Mode can lead to spills or air in the tubing To exit RUN Mode press RUN and confirm your intent to exit by pressing 2 for Yes and then ENTER The Main Menu display will appear To exit STANDBY Mode press STANDBY and confirm your intent to exit by pressing 2 for Yes and then ENTER The Main Menu display will appear Main Menu display Please select instrument mode RUN STANDBY MENU Prev
96. eter MeasParameter is an abbreviation for measurement parameters From this menu you may select the sample size 5 65 microliters You may also specify the locations station of calibrator solutions which is especially useful in certain dual chemistry applications From this menu you will also confirm or change specific parameters dealing with the black probe single channel unit or black and white probes dual channel unit These parameters include chemistry assignment calibration value units of concentration and time to endpoint of reaction NOTE YSI has assigned default values for each of these parameters but provides the flexibility for the user to change values to optimize a particular application DURING INITIAL SETUP YSI RECOMMENDS THAT YOU USE THE DEFAULT SETTINGS FOR THE CHEMISTRIES YOU CHOOSE Press 0 to back up then press 3 for RunMode The display will read RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor 3 RunMode In this menu you select parameters related to sampling protocol set parameters that control automatic switching to standby mode and select parameters that will trigger auto calibrations In Sample Protocol you can select the sample station position activate replicate sampling and sample identification prompts set the position to which the Sipper descends when sampling at the Manual Station and set turntable parameters if appropriate Press 0 to back up then press 4 for
97. ethanol 2 50 2500 0 250 78 1 32 2 38 2375 0 238 74 2 26 25 26 250 2 63 76 8 Sucrose 25 00 25 000 2 50 73 1 342 23 75 23 750 2 38 69 4 NOTE The linearity concentration ranges for each chemistry shown top to bottom as upper limit theoretical and lower limit for each of four concentration units FCN Membrane Integrity Test Concentration Unit Conversion mg L mw Chemistry g L ppm w v mmol L g mole Choline 0 02 15 0 02 0 14 104 Dextrose 0 05 50 0 01 0 28 180 Ethanol 0 05 50 0 01 1 09 46 L Glutamate 0 06 58 0 01 0 40 146 L Glutamine 0 06 58 0 01 0 40 146 L Lactate 0 03 30 0 01 0 34 89 Lactose 342 Methanol 0 05 50 0 01 1 56 32 Sucrose 0 10 100 0 01 0 29 342 NOTE Use the values from the preceding tables only when calibrating with the appropriate YSI calibrator solution Choline 2772 Dextrose 2776 Ethanol 2790 L Glutamate 2755 L Glutamine 2736 L Lactate 2776 Methanol 2726 and Sucrose 2780 See Section 3 Basic Operation Daily Operational Checks for cross references Appendix B YSI Supplies And Reagents Enzyme Membrane Kits YSI 4 2329 2365 2786 2701 2702 2703 2725 2735 2754 2771 Product Description L Lactate Membrane Kit Dextrose Membrane Kit Ethanol Membrane Kit Blank Membrane Kit hydrogen peroxide Galactose Oxidase Membrane Kit Sucrose Membrane Kit Methanol Membrane Kit L Glutamine Membrane Kit L Glutamate Oxidase M
98. ethod 3 Black 4 White Press 2 for CalMethod Select calibration method One Station 1 One Station 2 Two Stations Press ENTER to confirm One Station since both dextrose and L lactate are in one calibrator bottle and reside in one location Enter Cal Station gt 1 Press ENTER to confirm that the calibrator solution YSI 2776 is installed at Station 1 Cal Well of the Sample Chamber The display now returns to the Measurement parameter setup menu level Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 3 for Black At this level you will choose chemistry and other black probe measurement parameters Select BLACK chemistry L Lactate 0 Backup 1 Next chemistry ENTER Accept Press 1 for Next chemistry Use this key to scroll through the choices in the menu For our example choose L Lactate then press ENTER to confirm and move on to the next parameter Select BLACK unit of measurement g L 1 mmol L 2 mg L ppm 3 g L 4 w v Press 1 for mmol L This changes the g L default setting Select BLACK unit of measurement mmol L 1 mmol L 2 mg L ppm 3 g L 4 w v Press ENTER to confirm and move to the next parameter Enter Black calibration value in mmol L gt 05 62 The value you observe 5 62 mmol L is equivalent to 0 50 g L the concentration stated on the YSI 2776 calibrator label NOTE If you are using the default values and you choose a concentra
99. f they pass the tests in Section 3 3 it is not necessary to replace them It is important that the membranes do not dry out If the chamber is to be disassembled from the instrument for more than 15 minutes the membranes should be replaced Unscrew the two thumb nuts on top of the sample chamber Remove the sample chamber and waste chamber from the base plate see Figure 7 1 You will need to disconnect the calibrator well tube and the drain tube Be careful not to discard the small magnetic stir bar An extra one is supplied in your maintenance kit Clean the chambers with the appropriate disinfecting agent After cleaning flush the chambers with copious amounts of warm water then rinse with distilled water to remove any traces of the disinfecting agent 7 4 Clean up any salt deposits or fluid on the base plate Be sure that the base plate and all other parts are dry Refer to Figure 7 1 and reassemble the chamber Remember to return the stir bar to the chamber and be sure to install the chamber seal O ring Insert the probes into the chamber installing new membranes see Section 2 4 or O rings as necessary Extra O rings are supplied in your Preventive Maintenance Kit Connect the calibrator well tube and drain tube Refer to Section 2 6 to check the Sipper alignment and prime the buffer and calibration systems AUXILIARY ELECTR TEMPERATURE PROBE JES U RING E PROBE HOLDER NZYM MEMBRANE ENZYME PROB Figure 7 1 Sam
100. fications are based on the default settings shown above WARNING When using autocalibration parameters other than the default values described above you may compromise precision and or accuracy It is your responsibility as a user to verify performance through appropriate quality assurance testing These selections are provided as part of the overall concept of the 2700 SELECT flexibility You may have an application where 5 precision is acceptable if you can guarantee that the instrument will not initiate a calibration at a critical sampling time This menu allows you to make that possible The 5 autocal parameter displays are shown below with their default values The range of values that may be entered is shown below each display Press ENTER to confirm each parameter and proceed to the next Press 1 for Temperature Enter allowable autocal temp drift in C 0 to disable gt 1 Confirm value shown or enter and confirm number of degrees from to 20 5 14 Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Press 2 for Time Enter autocal time interval in minute gt 15 Confirm value shown or enter and confirm any number of minutes from 0 to 30000 Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Press 3 for Sample Enter number of samples between autocals 0 to disable gt 5 Confirm value shown or enter and confirm any number of samples from 0
101. full of buffer If necessary press 1 for Flush The Buffer Pump will turn on and flush buffer through the Sample Chamber Watch the baseline nA values to see if they are decreasing during the flush NOTE Other options in this mode 2 Calibrator and 3 Sample are used to observe the probe current after injection of calibrator and sample solutions respectively You should not need to use these probe diagnostics at this point Once the baseline currents are below 6 nA and reasonably stable press MENU to return to Main Menu You may need to allow an hour or more to establish stability when initially setting up the 2700 SELECT Please select instrument mode RUN STANDBY MENU 2 25 2 26 Once the installed membranes and probes are equilibrated the probe baseline current typically runs below 2 nA This equilibration may take a day or two and does not usually affect operation during the first day since autocalibrations compensate for probe current drift When enzyme membrane replacements are required in the future stabilization will occur much more rapidly usually within several minutes of installation if there has been no power disruption to the instrument 3 Basic Operation 3 1 Main Menu When you power on or reset your 2700 SELECT the Main Menu appears on the instrument display Please select instrument mode RUN STANDBY MENU From Main Menu you may enter any of three modes by pressing the appropriate function
102. hanges with newly installed reagents or enzyme membranes You may also elect to choose Detail report for the first few calibrations or samples after a long idle period where no samples or cals are run If you suspect problems with the instrument you should run Detail reports to provide more information for troubleshooting See Appendix D for example report formats Press 0 to return to Select Setup Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default You have completed Report setup Press 5 for PrntSetup The display will read Printing instrument setup Please wait The instrument will print the setup information you have entered The information is an itemized list of the key parameters that you have chosen The printing will take about 60 seconds and use about 8 inches 20 cm of printer paper See Appendix C for a sample printout When printing is completed the instrument returns to Select setup menu 5 15 Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default You have completed Print Setup PrntSetup level Press 6 for Default The display will read Reset all system parameters No 1 No 2 Yes This menu allows you to reset all system parameters to the default settings that is those that reside in ROM Read Only Memory when the instrument is manufactured DO NOT DO THIS AT THIS TIME If you have been entering setup information for a particular app
103. ibration report A 2 in the ID column specifies monitor report A 3 in the ID column specifies parameter information request A back slash in the column preceding the carriage return CR indicates that more information from that reading continues on the next line If two lines of information are combined the first is black probe data the second is white probe data In single channel units this will not be a concern In Figure 9 4 several examples show how information for a dual channel 2700 SELECT may appear when transmitted Again the ruler line is for reference only and does not appear on the record The first example shows a multidrop 2700 report with the assigned address of 123 The second example shows a non multidrop 2700 report Note that the only difference between this and the first example is the node address field The third example shows a calibration report The sample ID field of a cal report is always The fourth example shows a monitor report Similar to the cal report the sample ID field in this report is always 2 9 6 1234567890123456789012345678901234567890123456789012345678901234567890 2 22 34 02 13 98 23 56 123 123456789 H202 12345 78 mmol L 0000 cr l 2 22 34 02 13 98 23 56 123 123456789 H202 12345 78 mmol L 0000 lt gt lt 1 2 22 34 02 13 98 23 56 123456789 H202 12345 78 mmol L 0000 lt gt lt 1 2 22 34 02 13 98 23 56 123456789 H202 12345 78 mmol L 0000 lt gt lt
104. ine error or other fatal system error The internal receiver buffer in the 2700 is 80 characters long A string longer than 80 characters without the terminating CR will cause this buffer to overflow When this occurs the 2700 will reset the receiver buffer ignore the previous 80 characters and start to receive the new command Most commands are 2 or 3 characters long Thus there is no real restriction with this buffer size 9 2 Communications Modes The 2700 can be placed in one of two communications modes Result Reporting Mode or Remote Control Mode The selection between modes is made by the remote host The default mode at power up is Result Reporting Mode 2700 Data Base The 2700 keeps a data base of 32 sample results and 1 calibration result in its battery backed RAM random access memory Only the most recent sample or calibration result is shown on the instrument display However a remote host can ask for any of these results at any time This data base can be cleared by the host in Remote Control Mode see Section 9 3 Communications Commands Result Reporting Mode Result Reporting Mode is designed for use with a computer system that gathers sample results from various analyzers and records the results in a central data base In this mode the host can always talk to the 2700 and get the result records The host requests the results by either sending the sample ID number and getting the result record or by querying the unit
105. ions of other amino acids and related substances showed little or no interference On a scale where Glutamate 100 those substances that produced more than 0 5 response were L Aspartate 0 7 L Tyrosine 1 4 L Histidine 0 7 and L Glutamine 1 1 Glutamate cannot be ruled out as a contaminant in the Glutamine solution 4 14 L Glutamine This is a direct reading of glutamine in solution at the enzyme sensor Two enzymes are co immobilized in the YSI Glutamine Membrane Glutaminase and Glutamate Oxidase L Glutamine gt L Glutamate NH Glutaminase L Glutamate o Ketoglutarate NH Through this chain of reactions the amount of hydrogen peroxide liberated is directly proportional to the amount of glutamine System Buffer YSI 2357 Calibrator Std YSI 2736 5 00 mmol L Linearity Std YSI 2737 8 00 mmol L Black Probe YSI 2735 Glutamine Membrane White Probe n a Sample Size 20 uL Cal Station Station 2 Black Probe Parameters Chemistry Glutamine Unit of Conc mmol L Cal value 5 00 End Point 30 sec If you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations If sample dilution is required use reagent water or 2357 buffer The sample must be glutamate free or at least contain levels low enough not to interfere with the gl
106. ive assist with initial setup and orientation YSI warranty and product performance claims however are not dependent upon installation by factory or dealer personnel Unpacking and Notes on Safety Remove the instrument from the shipping container Be careful not to discard any parts or supplies Check off all items on the packing list and inspect all assemblies and components for damage In the event of damaged or missing parts contact YSI Customer Service or your Dealer Representative immediately Note that reagents for the 2700 SELECT Analyzer are not packaged in the same carton as the instrument These materials must be ordered separately as starter supplies and will arrive in a separate package DO NOT PLUG THE INSTRUMENT IN AT THIS TIME You should apply power only when directed to do so in the setup instructions If you ordered the 2700 SELECT with the 2710 Turntable first set up the 2700 SELECT Once the 2700 SELECT is operating properly refer to the YSI 2710 Turntable Operations Manual Notes On Safety Electrical precautions 1 BEFORE connecting the power cord check the line voltage selector and confirm that the selected voltage matches the local power supply Section 2 5 2 Use ONLY the line power cord supplied with the instrument Connect the plug to a matching three pronged wall receptacle 3 Use ONLY fuses of the type supplied Section 7 9 Replacement power cords and fuses can be obtained from YSI or your Dealer
107. key The modes are RUN STANDBY and MENU You may also transfer from one mode to another using the function keys however there are limits to what submenus you can access Refer to Figure 3 1 If you press RUN from Main Menu the instrument initializes and self calibrates Once calibrated it maintains a sample ready status If 2 hours pass without a sample being processed the unit automatically transfers to STANDBY Mode where reagents are conserved In Section 5 you will learn how to change this 2 hour threshold value to better fit your application If you press STANDBY from Main Menu the instrument flushes buffer through the Sample Chamber that houses the Enzyme Electrodes It then continues to flush buffer once an hour to maintain fresh solution in the Sample Chamber If you transfer from STANDBY to RUN Mode a calibration is initiated to update the calibration reference value stored in memory If you press MENU from Main Menu you enter a series of submenus that allow you to reconfigure your setup parameters perform service functions and utilize diagnostic routines You will learn about MENU selections in Section 5 Please select instrument mode RUN STANDBY MENU MENU STANDBY Y STANDBY B STANDBY MODE RUN i CALIBRATIUN Figure 3 1 2700 SELECT Software Structure 3 1 3 2 IMPORTANT The RUN and STANDBY keys act as toggle keys You ent
108. l calibrator prepare a solution with ionic strength Level sensing at Station 2 Test Tube Holder depends on a conductive solution A 0 1 K EDTA solution as a diluent acts as both a conductive solution and a preservative Normal saline solution 0 996 is also an acceptable diluent Your sample should be ethanol free Ethanol can be a significant interference since it is a good substrate for Alcohol Oxidase Propanol and butanol are very weak substrates of Alcohol Oxidase and usually do not present an interference problem Controlling evaporation of methanol from both sample and calibrator is important The use of some type of test tube cover eg prepunctured film will help Frequently replacing the calibrator solution with fresh solution will also minimize the effects of evaporation on measurement integrity 4 16 Simultaneous Dextrose and L Lactate You must have a dual channel version of the 2700 SELECT in order to perform this measurement Refer to the sections above on DEXTROSE and L LACTATE for theoretical and special considerations Then follow the instructions below to set up your instrument for simultaneous determination Note In Section 2 of this manual the step by step procedure for setting up this simultaneous measurement was described System Buffer Calibrator Std Linearity Std Black Probe White Probe Sample Size Cal Station Black Probe Parameters Chemistry Unit of Conc Cal value End Point White Probe Pa
109. lay 3 Printer Press 1 for Keypad You may now test each of the 20 keys on the keypad There are 12 numeric keys including space and backspace and 8 function keys To exit the keypad test press and hold CANCEL Hit keys Hold CANCEL to exit Key When you press and immediately release a key we refer to this as a hit The following will be displayed example is RUN key Hit keys Hold CANCEL to exit Key RUN hit When you press and hold a key down and then release it the following display messages should be observed Hit keys Hold CANCEL to exit Key RUN held down Hit keys Hold CANCEL to exit Key gt RUN released To exit the keypad test press and hold down the CANCEL key Hit keys Hold CANCEL to exit Key gt CANCEL held down The following message appears after you exit the keypad test I O diagnostic 1 Keypad 2 Display 3 Printer Press 2 for Display You should now see 95 different characters including a blank appear in each of the 40 segments of the liquid crystal display The total test takes about 40 seconds Terminate the test at any time by pressing any key The first few characters are shown below qot 1119119 T1 TT TT TT TT T TT TT TT T TTTTTTTTTTTTTTTTTY TECECELELELECETECETELELELELETETECELEDEDELCULCLCELELELELECELELELELELELCUI TLCECELELELELETECETELECELELCULEECECEDEDELCOLOLCELELELELELECELELCELELELCUI THHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
110. le concentration If you prepare your own ethanol calibrator prepare a solution with ionic strength Level sensing at Station 2 Test Tube Holder depends on a conductive solution A 0 1 K EDTA solution as a diluent acts as both a conductive solution and a preservative Normal saline solution 0 9 is also an acceptable diluent Your sample should be methanol free Methanol can be a significant interference since it is a good substrate for Alcohol Oxidase Propanol and butanol are very weak substrates of Alcohol Oxidase and usually do not present an interference problem Controlling evaporation of ethanol from both sample and calibrator is important The use of some type of test tube cover eg prepunctured film will help Frequently replacing the calibrator solution with fresh solution will also minimize the effects of evaporation on measurement integrity 4 9 4 9 Starch This is an indirect reading of starch by measuring dextrose liberated from the hydrolysis of starch To use the method described your sample must be dextrose free If free dextrose is present see Special Considerations below First dissolved starch is hydrolyzed externally by the enzyme Amyloglucosidase to produce dextrose Dextrose is read at the enzyme sensor The enzyme Glucose Oxidase is immobilized in the YSI Dextrose Membrane Starch H O B D Glucose dextrose Amyloglucosidase Dextrose O z2 H O D Glucono 6
111. lication resetting to Default will clear some or all of your selections and restore Default system parameters You will likely use Default to return to YSI recommended settings if you have been experimenting with the selections and would like to start at the beginning Press ENTER to confirm your choice and return to Select setup Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 0 to return to Select instrument function Select instrument function 1 Service 2 Setup 3 Diagnostic YOU HAVE NOW COMPLETED THE SETUP MENU OF THE SOFTWARE As you gain more familiarity with the 2700 SELECT menus the setup will not seem so complicated You will learn to jump in and out of the menus quickly sometimes not even needing to exit RUN or STANDBY mode to make a change When you have completed Service Setup or Diagnostic functions press 0 or MENUJ to return to Main Menu From Main Menu you may enter RUN or STANDBY modes Please select instrument mode RUN STANDBY MENU 5 4 Diagnostic Selections Diagnostic menu functions are used primarily during troubleshooting In this menu you will learn to gain individual control of components in the instrument For example you can turn motors on and off and even exercise motor controlled devices You can run pumps to check fluid movement You can check probe electrical current and even simulate a calibration or sample cycle You can check I O inp
112. ll and clear obstruction Reset instrument if required 2 6 SYMPTOM POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION SYMPTOM POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION POSSIBLE CAUSE ACTION SECTION ERROR Sample Chamber Temperature Out of Range Ambient temperature too cold or hot Operate at ambient temperatures between 15 and 35 degrees C ERROR Sipper Interference or Fault in Fluid Level Detector Sipper detects a conductive body Repeat sample Intermittent sipper ground cable Replace sipper ground cable 7 8 Intermittent sipper cable Replace sipper cable See the 2700 SELECT SERVICE MANUAL ERROR Test Tube Interference Sample tube holder not in position Reposition and repeat sample ERROR Unable to Calibrate Pinched leaking or disconnected tube Fix or replace tubing 7 6 Sipper misaligned Check Sipper alignment 2 6 Stir bar not in chamber Disassemble chamber and reinstall stir bar 7 3 Newly installed enzyme membrane Enter probe diagnostics and check probe currents 2 6 Newly installed probe Enter probe diagnostics and check probe currents
113. ll now reflect the concentration of choline hydroxide in the sample Assumptions and calculations are as follows choline hydroxide FW z 121 2 free choline FW 104 2 121 1 104 2 x 175 mg L 203 55 204 mg L rounded up Equivalent values for other choline salts may be calculated in a similar manner Potential Interferences Tests with equimolar concentrations of various vitamins and other ingredients of nutritionals showed little or no interference On a scale where Choline 100 those substances that produced more than 0 5 response were Riboflavin 1 1 Pyridoxine 1 5 Ascorbic Acid 0 8 and Thiamine 1 3 Vanillin an artificial flavor ingredient may produce interference levels exceeding 10 mole mole and should be tested separately for your individual formulations if known to be present 4 11 4 11 Galactose This is a direct reading of galactose in solution at the enzyme sensor The enzyme Galactose oxidase is immobilized in the enzyme membrane Galactose H O Galactose Dialdehyde Derivative Gal Oxidase System Buffer YSI 2705 Calibrator Std n a Linearity Std n a Black Probe YSI 2702 Galactose Oxidase Membrane White Probe n a Sample Size 25 uL Cal Station Black Probe Parameters Station 1 Cal Well Chemistry Galactose Unit of Conc g L Cal value user s choice recommend about 2 g L End Point 30 sec YSI does not currently offer galactose standard solutions Tf
114. mes self calibrate several times until a stable calibration is established In Section 5 3 you will learn to change some default calibration parameters to better fit your application Once a stable calibration is established the following display will appear Ready to sample at Station 2 You are now ready to run a sample You may present a sample at any one of several stations Station 2 Test Tube Holder Station Station 3 Manual Station Station 4 with optional 2710 Turntable or Station 5 with optional 2730 Monitor and Control Accessory When the Ready to sample screen first appears the sample station entered at the Run Mode Setup menu is selected However you may temporarily assign sampling to an alternate station simply press the number key corresponding to the number of the station you want to sample from For example press the 3 key to change the prompt from the previous display to Ready to sample at Station 3 The 2700 will sample from the alternate station until you re assign sampling or leave the Ready to sample screen Note that if an alternate sampling station is selected when you leave the Ready to sample screen the alternate setting will be lost and the station stored at the Run Mode Setup menu will automatically be selected the next time you return To permanently assign sampling to a different station you must enter the Run Mode Setup menu and store a new station in memory
115. mistry measurements that have been developed for the 2700 SELECT First we will briefly discuss the principle of using immobilized oxidase enzymes Secondly we will focus on each standard chemistry setup for single channel and then dual channel configurations Principle You may want to use Section 6 1 to help understand the information presented below regarding immobilized enzyme sensor technology You may find Figure 6 1 especially useful YSI s enzyme sensor technology employs one or more enzyme catalyzed reactions to ultimately produce hydrogen peroxide Hydrogen peroxide H O is electrochemically oxidized at the platinum anode of an electrochemical probe This produces a probe signal current In many procedures utilizing enzymes the enzyme is discarded with the sample after the analysis is made YSI s technology allows preservation of the enzyme by immobilizing it in a patented membrane structure The enzyme membrane is coupled with an electrochemical probe and housed in a sample chamber where sample delivery and flushing occur The life of the enzyme membrane is measured in days or weeks and is not dependent on the number of samples analyzed If used frequently you may measure hundreds of samples with a single enzyme membrane before its function is compromised In the sections below each of the chemistries available by this technology will be discussed In enzymology the terms substrate and product are commonly used to describe a r
116. n at the enzyme sensor The enzyme L Gluta mate Oxidase is immobilized in the YSI Glutamate Membrane L Glutamate gt HO a Ketoglutarate NH Glut Oxidase System Buffer YSI 2357 Calibrator Std YSI 2755 5 00 mmol L glutamate Linearity Std YSI 2756 10 00 mmol L glutamate Black Probe YSI 2754 Glutamate Oxidase Membrane White Probe n a Sample Size 25 uL Cal Station Station 1 Cal Well Black Probe Parameters Chemistry Glutamate Unit of Conc mmol L Cal value 5 00 End Point 30 sec f you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations lfsample dilution is required use reagent water or 2357 Buffer lfyou want to measure MSG monosodium glutamate monohydrate and express the results as mmol L of MSG the calibration parameters listed above are all appropriate If you want to express results as a w v measurement such as mg L MSG then correction for the sodium and water components is necessary To express results as an MSG value change the glutamate units to a w v expression and enter one of the following 936 mg L 0 936 g L or 0 094 96 Use YSI 2755 Calibrator Standard 5 00 mmol L Glutamate to calibrate the instrument Sample results will then reflect MSG concentration Potential Interferences Tests with equimolar concentrat
117. n clean the screw and guide rods with a lint free towel Apply Lubriplate 105 grease supplied in YSI 2788 Preventive Maintenance Kit to two or three threads of the Acme screw Normal operation will distribute the grease along the length of the screw Lubricate each guide rod with a drop of light machine oil Wipe off any excess lubricant promptly ES CCC COC NC Figure 7 9 Sipper Mechanism Drive Screw and Guide Rods 7 13 7 14 8 Troubleshooting This section provides a systematic approach to establishing the cause of an instrument malfunction Before taking any corrective action be certain you have collected as much pertinent information as possible To establish probable cause you should Review the printed reports for trends in data and errors Use the detailed format if possible to obtain as much information as possible An explanation of the report data is covered in this section Check reagent and membrane installation dates Compare the elapsed time to the recommended time Look and listen for problems fluid leaks salt build ups air bubbles in the chamber loose connections noisy components etc Review Section 5 4 Menu Diagnostics to learn more about how you can test individual components of the 2700 SELECT Use the troubleshooting chart in this section to assist you in identifying the pr
118. n the left side of the buffer pump then rotate the roller assembly while pushing the tubing down into the pump ahead of the roller Once the tubing is installed in the pump insert that tab on the rear pump housing into the slot at the top of the front pump housing see Figure 7 4 Lower the front pump housing while guiding the tubing into the slots at the bottom Gently squeeze the two housings together while pushing up on the roller assembly Align the slot in the rear of the pump with the motor shaft then slide the pump onto the shaft Remount the pump to the instrument wall using the mounting screws pull up on the pump to ease installation of the screws Remember to install the tube bushing on the right side of the pump 7 7 7 8 Rear Housing Roller Assembly Tubing Front Housing C 4 Tube Bushing Figure 7 4 Pump Assembly When all tubes are properly connected see Section 2 6 to readjust the Sipper and prime the calibrator and buffer systems Sipper Replacement The Sipper can be damaged if it is not properly aligned or if its alignment is disturbed If your Sipper needs replaced remove the screw that mounts it to the sipper arm disconnect the tubing and remove the Sipper Install the new Sipper Reconnect the Sipper ground cable and tubing to the new Sipper Refer to Section 2 6 to readjust the Sipper Sipper Ground Cable The Sipper Ground Cable flexes with every sample After several thousan
119. nel reserve the right to withhold service until appropriate cleaning decontamination and certification has been completed We will contact the sender for instructions as to the disposition of the equipment Disposition costs will be the responsibility of the sender When service is required either at the user s facility or at YSI the following steps must be taken to insure the safety of our service personnel In a manner appropriate to each device decontaminate all exposed surfaces including any containers 70 isopropyl alcohol or a solution of 1 4 cup bleach to 1 gallon tap water are suitable for most disinfecting Instruments used with waste water may be disinfected with 596 Lysol if this is more convenient to the user The user shall take normal precautions to prevent radioactive contamination and must use appropriate decontamination procedures should exposure occur If exposure has occurred the customer must certify that decontamination has been accomplished and that no radioactivity is detectable by survey equipment Any product being returned to the YSI Repair Center should be packed securely to prevent damage Cleaning must be completed and certified on any product before returning it to YSI Packing Instructions DOR abe Clean and decontaminate items to insure the safety of the handler Complete and include the Cleaning Certificate Place the product in a plastic bag to keep out dirt and packing material Use a large
120. ng system The Remote Communication Port is an RS 232 serial port It is used to interface with host computers or other laboratory instruments The Auxiliary Port is an interface connector for use with accessories like the YSI 2710 Turntable 2 3 2 4 WARNING SHOCK HAZARD EXISTS W NO USER SERVICEABLE AGE SELECTOR Figure 2 2 Back View of the 2700 SELECT The Line Voltage Selector selects either 110 120 or 220 240 volt operation It houses the fuses and pulls out of the case for fuse replacement The Power Receptacle is a power inlet One end of the power cord plugs into this receptacle while the other end plugs into an electrical outlet The Power Switch is an on off toggle switch 0 off and I on It is located on the back of the instrument 2 3 Reagent Preparation Prepare the supply buffer and fill the buffer bottle Powdered buffer concentrate is included in the starter supplies YSI 2357 is recommended for use with all YSI Enzyme Membranes except YSI 2702 Galactose Oxidase membranes use YSI 2705 YSI 2786 Ethanol Membranes use YSI 2787 and YSI 2725 Methanol Membranes use YSI 1579 All YSI buffers may be ordered separately Place about 500 mL of reagent water distilled and or deionized into a one liter flask or other clean container Add two packages of YSI 2357 Buffer Concentrate and stir NOTE YSI 1579 Carbonate Buffer Concentrate used with 2725 membranes
121. nment Ambient temperature Relative humidity Instrument dimensions Instrument weight Adjustable from 5 to 65 microliters aspirated volume Sample results in 60 seconds Complete sample to sample cycle in 90 seconds May vary with analyte and sample matrix 110 120 VAC or 220 240 VAC 50 60 Hz 50 Watts nominal 15 to 35 Celsius 10 to 90 non condensing 10 0 x 14 0 x 14 0 inches 25 4 x 35 6 x 35 6 centimeters 25 pounds 11 4 kilograms Chemistry Performance Specifications Dextrose D Glucose YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV n 10 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring Color Ethanol YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV n 10 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring Color Potential Substrate Interference L Glutamate YSI Membrane Detection Range Calibration Point Linearity Check Point Precision CV n 10 Linearity 0 to Cal Point Linearity Cal to Range Max Typical Membrane Working Life Membrane O ring Color 2365 0 9 g L at 25 uL sample size 0 25 g L at 10 uL sample size 2 50 g L 9 0 g L 2 2 5 21 days Red 2786 0 3 2 g L 2 00 g L 3 20 g L 2 2 t5 5 days Green Methanol 2754 0 10 mmol L 5
122. nt that you note which probe black or white you use to install specific membranes It will be necessary to assign chemistry to probe during instrument parameter programming For single channel configuration the YSI Enzyme Membrane must be installed on the black probe The blank is sealed in the chamber You need not remove it To install a membrane first unscrew the appropriate probe retainer and gently pull the probe out of the block Remove the existing O ring membrane assembly from the end of the probe A toothpick or pointed tool may be needed to unseat the old membrane Be careful not to scratch the probe face Examine the probe surface and remove any pieces of membrane that remained Open a cavity of the plastic membrane holder and rinse the membrane inside with a few drops of salt solution YSI 2392 or equivalent Place one drop of salt solution on the probe face Using the plastic membrane holder press the O ring membrane assembly gently onto the probe face Figure 2 5 Wipe excess salt solution from the probe body then return the probe to the sample chamber Finger tighten the probe retainer so that the O ring seals the probe in place Do not overtighten Repeat this procedure for the second probe if you have a dual channel unit Return the membrane holder to the foil pouch and refrigerate it Note the expiration date on the membrane package It is advisable to maintain an instrument log book in which dates and lot numbers of reagents
123. oblem then use the chart to guide you to a corrective action If the problem cannot be resolved contact YSI Customer Service address and phone information in Appendix F When communicating with service personnel please indicate the serial number of the instrument If writing or transmitting a FAX for assistance please include a thorough description of the problem and copies of printouts if possible Printout Information Refer to Appendix D to learn more about report format options for the 2700 SELECT For troubleshooting or even daily log information the detail report format is preferable The Detail Report provides a complete description of the sensors for a calibration or sample Information for both the black and white probes as well as the temperature probe is included You may access the report format level without exiting RUN or STANDBY modes Press MENU on the keypad to display the Select setup menu level Press 4 for Report to change or confirm sample and calibration formats Listed below are example printouts and explanations of the Detail format information Sample Report Detail Calibration Report Detail Sample Report CALIBRATION REPORT ID 123456789 05 03 B Lactate Unstable B L Lactate 4 82 g L IB current 1 79 nA IB current 2 11 nA PL current 11 7263 nA PL current 118 65 nA FB current 1 51 nA Slope 4 69 nA min Base shift 2 65 End Point 30 Sec Slope 1 42 nA min W
124. ode 1 2700 is not in Remote Control Mode 2 2700 is busy in Run Mode When you invoke TN1 Enter Run Mode the instrument enters Run Mode from Standby or from Menu Mode You may use this command to re enter Run Mode after using the TNO command or to reinitialize the instrument after a halt condition such as an error resulting from unstable calibration or similar problems Host to 2700 TNI Enter Run Mode from Standby or Menu mode 2700 to Host A Acknowledge 2700 switches to Run Mode 1 2700 is not in Remote Control Mode 9 2700 is not in Run Mode but is attempting to enter Run Mode from a halt condition IMPORTANT These commands are designed to be used when the 2700 is in Remote Control mode In writing control programs verify that the instrument is in Remote Control mode before invoking these commands The calibration commands and sample process commands are outlined below The process sample commands are described together the only difference among the different commands being the station from which the sample is aspirated 1 2 4 5 Station 3 is theoretically possible but not practical in remote control mode When you invoke the Process Calibration PC command the Host commands the 2700 to run a calibration cycle If the 2700 is running an autocalibration calibration stabilization or baseline stabilization the command is acknowledged and the sample is processed as soon as the instrument finishe
125. oint 30 sec YSI does not currently offer hydrogen peroxide standard solutions f you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry Note See Appendix A if concentration unit conversion is required Special Considerations Since hydrogen peroxide is typically used to calibrate the YSI Blank Membrane the enzyme catalase is a concern Catalase destroys hydrogen peroxide Catalase occurs naturally and may be in the sample you are testing If so it can also accumulate in the 2700 SELECT sample chamber A small amount of sodium azide added to the system buffer will inactivate the catalase Concentrations around 5 mg L sodium azide are appropriate If you have a dual channel 2700 SELECT and suspect an electrochemical interference as opposed to an enzymatic interference you may want to use one channel to qualitatively monitor for an interference effect Typical electrochemical interferences include phenols mercaptans hydroxylamine hydrazine and analines If you suspect a particular substance you may want to configure one of your probes with a blank membrane and calibrate it as described above under Hydrogen Peroxide Then run your sample and check for activity at the Blank Membrane It is not quantitative but may provide useful information about your sample 4 13 4 13 L Glutamate L Glutamic Acid This is a direct reading of L glutamate in solutio
126. on reference point after every calibration If a difference of more than 2 default setting between the present and previous net calibration currents occurs the instrument repeats calibration The sensors net current for a calibration is displayed and printed An unstable calibration is displayed and printed as a cal shift While establishing a stable calibration the 2700 SELECT will run 5 calibrations before the error unable to calibrate is displayed The flexible parameter selection allows the user to disable this error mode in menu setup In summary by the default calibration settings recalibration will occur after every 5 samples or 15 minutes after a calibration shift of 2 or greater or after a sample chamber temperature drift of more than 1 C After 5 attempts without successfully calibrating the instrument displays a calibration error message Linearity As discussed in Section 6 1 a sensor consists of an electrode and an enzyme membrane As a membrane ages its response becomes non linear shown in Figure 6 4 i Dld Membrane Figure 6 4 Aging Membrane Response 6 7 6 8 Under optimal conditions the sensor response depends on diffusion limitation of the substrate When the substrate can diffuse at a greater rate than the enzyme can turnover product enzyme kinetics defines the response and nonlinearity is a symptom This occurs as an enzyme membrane ages It is necessary to periodically check sensor linea
127. on required to run the 2700 SELECT for your particular application You will set general information such as date time display and print formats You will enter specific information related to chemistry selection calibration values concentration units calibration and sampling protocols communication parameters and other features Once set the parameters are maintained in memory In the event of power loss the 2700 SELECT has battery backup to preserve this information NOTE When first powered the instrument will contain most of the general information you need Some information is set prior to delivery and preserved by battery and other information will be set as default values which are appropriate for many standard applications Use the display messages and text below to learn the flexibility you have in setting up your 2700 SELECT You may find it helpful to use the Menu Flow Chart Figure 5 2 to follow the menu levels Main Menu display Please select instrument mode RUN STANDBY MENU From Main Menu press MENU to display instrument functions Select instrument function 1 Service 2 Setup 3 Diagnostic Press 2 for Setup to display setup selection categories Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 1 for General The display will read General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor In General setup menu you may
128. option to confirm that you have correctly setup your instrument Remember PrntSetup can be accessed from Main Menu by pressing MENU then 2 for Setup and then 5 for PrntSetup Again refer to Figure 5 2 Menu Flow Chart for an overview 2 10 Probe Baseline Check You were previously instructed to prime the fluid system Since then you have been learning about the menu selections Now it is time to check the probe baseline current to determine if the installed enzyme membrane sensor s and probe s have equilibrated and are stable enough to initiate calibration From the previous instructions your instrument should be displaying Main Menu Please select instrument mode RUN STANDBY MENU If your instrument display shows another message press MENU on the keypad The instrument should display the message above If you inadvertently entered the Run Mode or Standby Mode you will need to exit to return to the Main Menu See instructions in Section 5 1 regarding entering and exiting these modes Press MENU to display instrument function options Select instrument function 1 Service 2 Setup 3 Diagnostic Press 3 for Diagnostic Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 3 for Probe B LAC 4 23 nA W DEX 3 10 nA 1 Flush 2 Calibrator 3 Sample Observe the probe current values If they are above 6 nA nA nanoamp check to see if they are decreasing in value Check the Sample Chamber it should be
129. out 500 nanoamps Baseline Stability The 2700 SELECT monitors the probe baseline current for nA activity and stability If an unstable baseline is detected the buffer pump will continue to flush the sample chamber with buffer When a stable baseline is established an automatic calibration is initiated After every calibration and sample the final baseline current ifb is compared to the initial baseline current iib during the flush cycle If a significant shift is detected the buffer pump continues to flush As soon as the baseline recovers buffer flushing ceases and the instrument performs its next command There is a limit of about 3 minutes at which time the instrument displays a baseline error message 6 5 6 6 Calibration To maintain a sample ready status the 2700 SELECT self calibrates Calibrating establishes the sensors response in nanoamps of current to a known concentration of substrate The sensors calibration response must be above 5 nA A response below this value will result in an error The 2700 SELECT self calibrates when entering RUN Mode While in RUN Mode it calibrates every 5 samples or 15 minutes However default calibration parameters can be altered to tighten or loosen calibration specifications In RUN Mode a manual calibration can be initiated by pressing CALIBRATE or by remote control through an RS 232 interface A STABLE CALIBRATION IS IMPORTANT The instrument re establishes a calibrati
130. overed by the membrane is situated in a buffer filled sample chamber into which a sample is injected Some of the substrate diffuses through the membrane When it contacts the immobilized oxidase enzyme it is rapidly oxidized producing hydrogen peroxide See Reaction 1 using glucose as an example The hydrogen peroxide is in turn oxidized at the platinum anode producing electrons Reaction 2 A dynamic equilibrium is achieved when the rate of HO production and the rate at which H2O leaves the immobilized enzyme layer are constant and is indicated by a steady state response Figure 6 3 The electron flow is linearly proportional to the steady state concentration and therefore to the concentration of the substrate REACTION 1 glucose B D glucose gt Glucono 6 lactone HO REACTION 2 gt 2H O 2e Pt anode The platinum electrode is held at an anodic potential and is capable of oxidizing many substances other than To prevent these reducing agents from contributing to sensor current the membrane contains an inner layer consisting of a very thin film of cellulose acetate This film readily passes HO but excludes chemical compounds with molecular weights above approximately 200 The cellulose acetate film also protects the platinum surface from proteins detergents and other substances that could foul it However the cellulose acetate film can be penetrated by such com
131. ple Chamber Illustration Membrane Replacement To assure proper performance and prevent unexpected down time check membrane integrity and linearity on a daily basis Refer to Section 3 3 Daily Operational Checks for details When enzyme membrane installation is required refer to Section 2 4 replacement procedures The typical working life of an enzyme membrane is displayed under Chemistry Performance Specifications Section 1 4 You may want to use the expected life as a guideline for scheduling replacement 7 5 7 6 Probe Cleaning With normal use enzyme sensors may become fouled and cease to operate in a normal mode A fouled sensor s output current will decrease and calibration may become unstable Since the severity of fouling will vary listed below are methods of cleaning which should be matched to the cleaning needs Follow the steps carefully and in order Sensor Maintenance It is necessary to maintenance the enzyme sensor when the 2788 PM kit is installed and periodically as needed 1 Remove the enzyme membrane and hold the probe with the electrodes facing up 2 Wada small portion of a lint free tissue and wet it with 7096 isopropyl alcohol 3 Using your thumb press the alcohol soaked wad against the probe s surface and rotate the probe back and forth 4 Rinse the sensor with reagent grade water and return the sensor to normal service Sensor Cleaning and Reconditioning The following procedure is recommende
132. pounds as hydrogen sulfide low molecular weight mercaptans hydroxylamines hydrazines phenols and anilines 6 2 Fluid System The 2700 SELECT fluid and electromechanics system is schematically described below in Figure 6 2 VERT DRIVE PHOTOSENSOR HORI 7 DRI V E gt PHOTOSENSOR MANUAL SAMPLE CAL STATI DN STATI UN STATI UN 13 2 M T um Z HORI DRIVE AN NERT N SAMPI DRIVE Si pees CHAMBER MOTOR SUNE a PHOTOSENSOR BU R y A Noe 2 NN N N Pd ns TEST TUBE A MOTOR SYRINGE T Figure 6 2 2700 SELECT Fluid System 6 3 6 4 Measurement Methodology The 2700 SELECT employs a steady state measurement methodology A typical sensor response is shown in Figure 6 3 Figure 6 3 Typical Sensor Response When sample or calibration standard is dispensed into the chamber it is diluted into 600 microliters of buffer The sensor response increases and plateaus After several seconds the buffer pump flushes the chamber and the sensor response decreases The net response is the difference between the plateau current iplat and the initial baseline current iip Typical net responses for the 2700 SELECT are between 10 and 25 nA nanoamps for YSI calibration solutions The maximum net probe current is ab
133. r ID Prompt sample ID No 1 No 2 Yes Again our example setup requests that we use sample identification Press 2 for Yes Prompt sample ID Yes 1 No 2 Yes Press ENTER to confirm and return to Sampling protocol setup SamplingProtocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 4 for Station Enter Sample Station gt 2 The default station is Station 2 which is where our test tube will be held Press ENTER to confirm and return to Sampling protocol setup SamplingProtocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 0 to return to Run mode setup RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor 2 21 2 22 Press 2 for AutoStandby Enter autostandby time in hour 0 to disable gt 2 This entry defines the number of hours the unit will continue to update calibration and be ready to sample The default setting is 2 Note that you would press 0 then ENTER to disable autostandby which maintains the unit in a sample ready mode indefinitely For now press ENTER to confirm two hour autostandby and return to the Run mode setup RUN mode setup 1 SampleProtocol 2 AutoStandby 3 AutoCal 4 Monitor Press 3 for AutoCal Autocal setup 1 Temperature 2 Time 3 Sample 4 Cal shift 5 Sample error Each parameter in this menu level can be used to alter the conditions which trigger autocalibrations For now simply
134. rameters Chemistry Unit of Conc Cal value End Point YSI 2357 YSI 2776 2 50 g L dex 0 50 g L L lact YSI 1531 9 00 g L dex and YSI 1530 2 67 g L L lact YSI 2329 L Lactate Membrane YSI 2365 Dextrose Membrane 25 uL Station 1 Cal Well L Lactate g L 0 50 30 sec Dextrose g L 2 50 30 sec Note See Appendix A if concentration unit conversion is required 4 17 4 17 Simultaneous Dextrose and Sucrose You must have a dual channel version of the 2700 SELECT in order to perform this measurement Refer to the sections above on DEXTROSE and SUCROSE for theoretical and special considerations Then follow the instructions below to set up your instrument for simultaneous determination Since the Sucrose Membrane 2703 utilizes a chain of enzyme reactions and the final reaction is a dextrose measurement free dextrose in a sample will be measured at the Sucrose Sensor By using the information at the Dextrose Sensor this free dextrose is automatically subtracted in the instrument software The algorithm looks basically like this Total Dextrose Free Dextrose x 1 9 Sucrose Total Dextrose is the combined sucrose and dextrose probe signal Free Dextrose is the dextrose not derived from sucrose hydrolysis The constant 1 9 corrects for the weight of fructose that is not sensed at either probe and the water of hydrolysis System Buffer YSI 2357 Calibrator Std YSI 2776 and YSI 2780 Linearity
135. re to bring the plateau current into a reasonable range 4 3 4 4 Calibration Method 2 CalMethod In most chemistry setups you will select One Station and the calibration station will be Station 1 Calibration Well However you have options to configure this in other ways Select One Station but assign Cal Station 2 Station 2 is the Test Tube Holder Station For example you may place a 500 mg L dextrose solution in a test tube and calibrate in this manner If you choose this option the sampling position is normally Station 3 Manual Station With accessories Station 4 Turntable or Station 5 Monitor may be used If you are running a dual chemistry where a potential interference is involved you may select Two Station You will then be prompted to assign calibration stations for both black and white probes For example you could install a sucrose membrane on one probe and a dextrose membrane on the second probe You could place sucrose calibrator in the test tube position 2 and dextrose at the Cal Well 1 In this setup the instrument is programmed to measure the dextrose reading at the sucrose membrane and automatically subtract it Black Probe Parameter 3 Black In this menu you choose parameters that correspond to the chemistry of interest at the Black Probe Be certain that you install the correct enzyme membrane type The selections include Chemi
136. rity YSI offers linearity standards for all of the recommended calibration values See Section 1 4 for details If you chose to identify your own calibration value and measurement range keep this failure mode in mind Temperature Compensation The sensitivity of the sensors in the 2700 SELECT varies with temperature changes The temperature probe in the sample chamber monitors the fluid temperature very near to the enzyme sensor The sample results are temperature corrected for the difference in temperature between the sample and the calibration Level Sensing and Sipper Interference The 2700 SELECT employs capacitive level sensing on the Sipper and in the calibrator waste and supply bottles The Sipper level sensor detects the sample surface at the Test Tube Sample station and then travels into the sample about 3 millimeters 1 8 inch This controlled immersion depth permits the use of sample tubes that are filled to different heights without significant carry over between samples This same technique can be employed when using the optional 2710 Turntable See the YSI 2710 Turntable Operation Manual The Sipper and Arm Assembly should never be touched while the unit is in operation As an extra safety precaution the Sipper detects contact with a conductive body such as a hand If a conductive body is detected the Sipper immediately stops waits for several seconds and then returns to the Sample Chamber The calibrator and supply bot
137. rose If the reading falls outside these limits recalibrate the instrument by pressing CALIBRATE After recalibrating repeat the analysis as directed above If your reading is still outside this range proceed for now Later in this section specific actions for nonlinearity will be addressed Operation at the Manual Station 3 Remove the tube from the Test Tube Holder Rotate the Tube Holder back into place Next press 3 on the keypad Note the change in Station on the LC display Alternatively rotate the Test Tube Holder out to trigger the switch that senses a test tube The Sipper will automatically go to Station 3 Manual With the door fully closed press SAMPLE The display reads Manual sample pause moving MS Wait for the Sipper to come to a stop at the Manual Station then bring the sample up to the Sipper so the tip is just immersed about 3 millimeters 1 8 inch below the fluid surface See Figure 3 4 If the Sipper dips too deeply into the sample future measurements may be contaminated by carry over of excess sample on the outside of the Sipper Be very careful not to jar or push on the Sipper during manual operation You could disturb its critical alignment The display now reads Present sample to needle and hit SAMPLE to aspirate Press SAMPLE The sample will be aspirated and the Sipper will return to the Sample Chamber Do not move the sample container until the Sipper has returned the Sample Chamb
138. rval to t where t is in minutes MSs change monitor station number to s Abort Turntable Sample Command PA You may use this command to abort turntable samples in progress Recall you initiate the turntable sampling using the PS command You cannot use the 2700 SELECT CANCEL key to abort the run since the 2700 SELECT keypad is disabled in remote control mode 9 17 Appendix A Concentration Unit Conversion In the YSI 2700 SELECT menu selection procedure you have the option to assign units of concentration You will find this in the Setup Menu under measurement parameters black and white probes There are default values set based on calibration solutions offered by YSI Below is a table of unit conversions for these calibration solutions and other concentrations of interest mg L mw Chemistry g L ppm w v mmol L g mole Choline 0 18 175 0 018 1 68 104 Dextrose 2 50 2500 0 25 13 89 180 Ethanol 2 00 2000 0 20 43 48 46 Galactose 2 50 2500 0 25 13 89 180 L Glutamate 0 73 731 0 07 5 00 146 L Glutamine 0 73 731 0 07 5 00 146 L Lactate 0 50 500 0 05 5 62 89 Lactose 5 00 5000 0 50 14 62 342 Methanol 1 00 1000 0 10 31 25 32 Peroxide 0 60 600 0 06 17 65 34 Sucrose 5 00 5000 0 50 14 62 342 Denotes that YSI does not currently offer this standard value If you are preparing your own standard of a value not listed in the preceding table refer to the example conversions on the next page to help calculate yo
139. s the existing task Host to 2700 PC Process calibration 2700 to Host A Acknowledge Calibration in process or pending upon completion of current task 1 2700 is not in Remote Control Mode 2 2700 is busy processing last PC or PS command 9 13 When you invoke the Process Sample PS command the Host commands the 2700 to sample from one of four locations depending on the specific command PS1 PS2 PS4 or PS5 If the 2700 is running an autocalibration calibration stabilization or baseline stabilization the command is acknowledged and the sample is processed as soon as the instrument finishes the existing task Host to 2700 PS Process sample at PS1 PS2 PS4 or PSS 2700 to Host A Acknowledge Sample in process or pending upon completion of current task 1 2700 is not in Remote Control Mode 2 2700 is busy processing last PC or PS command 9 For PS2 command Test Tube Holder switch is off The PS command may take three optional arguments PS s t n In these arguments s is the station number from which to sample If s is not given or NULL the currently assigned sample station is used The character t is the turntable starting position which is only valid when s 4 Remember station 4 is the turntable sampling position If t is not provided or NULL the default starting position will be used The character n is the number of turntable positions to be sampled which also is only valid when s 4 If n is
140. s under study If possible account for any interferences to your reading For example if you want to measure starch free dextrose must be considered Methods to do these corrections are described below Once you make the above determinations you can decide whether one of the standard setups described below will be appropriate or whether you will need to customize your setup In either case the Measurement Parameter section of the instrument Setup Menu lists four submenus in which you must confirm or assign appropriate parameters These are sample size calibration method black probe parameters and white probe parameters If you have a single channel unit white probe parameters do not appear in the menu choices You may want to access the print setup command in your 2700 SELECT menu From Run or Standby mode press MENU then select 5 for PrntSetup From Main Menu display press MENU select 2 for Setup then select 5 for PrntSetup In either case the instrument printer will print a complete list of system parameters See Appendix C for more detail on accessing PrntSetup and also to review the sample print outs of a single channel unit set for dextrose and a dual channel unit set for simultaneous dextrose and L lactate analyses The parameters you see in these printouts are default values For many applications the default settings will be appropriate for you Now enter the Measurement Parameter menu press 2 for Meas
141. second run of the Cal pump Make certain that all air bubbles are out of the calibrator tubing when the new calibration solution is pumped to the Calibrator Well You may need to initiate a second run to insure the delivery of calibrator solution to the Calibrator Well 7 1 7 2 Please select instrument mode RUN STANDBY MENU Select instrument function 1 Service 2 Setup 3 Diagnostic Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable If the calibrator bottle cap assembly needs cleaning make sure it is thoroughly rinsed and dried before it is attached to a fresh bottle of calibrator solution If you are calibrating from a test tube at the Test Tube Holder Station 2 evaporation is a major concern Calibrating with calibrator that has experienced any significant evaporation will result cause inaccurate test results Consider changing calibrator daily or more often if required Check the Buffer Bottle Replace the buffer if the bottle is low or the buffer has been in the instrument longer than 1 week Follow the instructions in Section 2 3 Reagent Preparation You may find it convenient to make more than one liter of buffer at a time in order to have it on hand to replenish the buffer bottle Prepare it in a clean bottle with cap You should store it at room temperature After a buffer change prime the buffer system From Main Menu press MENU See menu information displayed above Next press 1
142. splay Please select instrument mode RUN STANDBY MENU NOTE You may press MENU from most levels of diagnostics to return directly to Main Menu You now have had a complete look at the Diagnostic Menu You may press RUN or STANDBY to enter an operating mode or remain in Main Menu indefinitely however note that no automatic calibrations or sample chamber flushes will occur in this mode 5 25 5 26 6 Principles of Operation 6 1 Sensor Technology The sensor technology of the YSI 2700 SELECT is based on the principles conceived by Dr Leland Clark of Children s Hospital Foundation Cincinnati Ohio The immobilized enzyme membrane was invented by YSI and is covered by U S Patent 4 073 713 This sensor technology has been used successfully since 1975 in the YSI 23A Blood Glucose Analyzer then later in the YSI 27 Industrial Analyzer and the YSI 23L Blood Lactate Analyzer Most recently the same technology has been employed in the YSI 2300 STAT PLUS Glucose and L Lactate Analyzer and the YSI 1500 SPORT Lactate Analyzer REACTION 2 SILVER PLATINUM d m ACETATE Ca MEMBRANE m OXIDASE EM NEM MEMBRANE O RING H2 02 eg ot Figure 6 1 Sensor Probe and Enzyme Membrane Each probe is fitted with a three layer membrane containing immobilized enzyme in the middle layer Figure 6 1 shows an exploded view of the membrane and its relationship to face of the probe The face of the probe c
143. ss ENTER to confirm and return to Sampling protocol setup Sampling protocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 3 for ID If you wish to assign sample identification numbers to each sample enter 2 for Yes here When you press SAMPLE in RUN Mode the display will prompt you to enter a sample ID number You may elect not to assign a number for each sample in which case the prompt display disappears and the sample is run identified only by time and date Prompt sample ID No 1 No 2 Yes Press ENTER to confirm and return to Sample protocol setup Sampling protocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable Press 4 for Station to assign the position at which the sample will be aspirated The default location is Station 2 the Test Tube Holder station You may also elect to sample from Station 3 the Manual Station or even Station 1 the Cal well This last choice would be rarely used but does give you the flexibility to calibrate from a solution in the Test Tube Holder 2 and sample from the Cal Well 1 If you have the YSI 2710 Turntable or YSI 2730 Monitor and Control Accessory stations 4 and 5 are also available See the appropriate manual for assignments Enter Sample Station M N Press ENTER to confirm and return to Sampling protocol setup Sampling protocol setup 1 SipperHeight 2 Replicates 3 ID 4 Station 5 Turntable CAUTION Do not press 5
144. ssing sample Processing calibration Processing autocalibration Processing manual sample Processing precal cycle Processing monitor cycle Processing postcal cycle Flushing and aborting error cycle Stabilizing baseline current Stabilizing calibration current Stabilizing motors Aborting Run Mode In Run Mode In Standby Mode In Main Menu Mode Idle no pending command Sam command received Pending Cal command received Pending Unknown The information stated is that the 2700 is under remote control C no sample results are in memory N calibration results remain unsent U the 2700 is idle in Run mode I and there are no remote commands pending I When Report Last Transmission RX is invoked the last transmission of the 2700 is sent to the host It is used when the response received by the 2700 contains an unknown character is not in the correct format or whenever in doubt due to communications error Host to 2700 RX Report last transmission 2700 to Host last transmitted record When Report Calibration Result RC is invoked the 2700 sends back time date chamber temperature probe chemistry and probe current from the last calibration cycle The calibration cycle may be a user activated calibration cycle computer demanded cycle or an autocalibration cycle If no result is found the 2700 sends back error code 9 Host to 2700 RC Report last calibration result 2700 to Host cal result see format in Figure 9 4
145. stry 10 selections plus Unit of Concentration g L mg L mmol L 96 w v Calibration value must correlate to units chosen End Point basically the reaction time in the sample chamber For most standard applications recommended parameter settings are outlined below under each chemistry setup White Probe Parameter 4 White In this menu you choose parameters that correspond to the chemistry of interest at the White Probe Be certain that you install the correct enzyme membrane type The selections include Chemistry 10 selections plus Unit of Concentration g L mg L mmol L w v Calibration value must correlate to units chosen End Point basically the reaction time in the sample chamber For most standard applications recommended parameter settings are outlined below under each chemistry setup 4 4 D Glucose Dextrose This is a direct reading of dextrose in solution at the enzyme sensor The enzyme Glucose Oxidase is immobilized in the YSI Dextrose Membrane Dextrose O H O D Glucono 6 Lactone Glu Oxidase System Buffer YSI 2357 Calibrator Std YSI 2776 2 50 g L dextrose Linearity Std YSI 1531 9 00 g L dextrose Black Probe YSI 2365 Dextrose Membrane White Probe n a Sample Size 25 uL Cal Station Station 1 Cal Well Black Probe Parameters Chemistry Dextrose Unit of Conc g L Cal value 2 50 End Point 30 sec Note See Appendix A
146. t grade water for at least 2 minutes Oy A dace Install a new membrane and return the probe to service Note Several hours may be required for the sensor to stabilize after cleaning 7 6 Tubing Replacement The buffer and calibrator pumps in the 2700 SELECT are peristaltic tubing pumps The tubing life depends on instrument usage The pump tubes should be replaced at least every 6 months more frequently if necessary Other tubes become loose at the fittings and should also be replaced every 6 months Ss SIPPER TUB AL WELL Pa CAL PUMP SIPPER PUMP CALIBRATOR SUPPLY Figure 7 2 Tubing Connections Remove the screws holding the buffer and calibrator pumps to the instrument wall and remove the pumps Remove the front housing from each pump by pulling it out at the bottom Remove the roller assembly and tubing from each pump Disconnect and remove all the instrument tubing New tubing is supplied in the Preventive Maintenance Kit Connect the new tubing as shown in Figure 7 2 When installing new pump tubing insert the roller assembly into the rear pump housing rollers facing out Starting on one side place the tubing between the pump housing and roller as shown in Figure 7 3 7 7 7 8 Figure 7 3 Pump Tubing Installation Make sure that the correct length of tubing is sticking out of the pump 1 to 1 2 5 to 3 5 cm of the large diameter tubing o
147. t you have entered into memory for running the 2700 SELECT See Appendix C for an example of this report When finished the display again shows the Select setup menu Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 6 for Default Reset all system parameters No 1 No 2 Yes This menu allows you to reset all system parameters to the default settings that is those that would appear if you were to unpower the instrument including backup battery power Do not select 1 Yes now Instead press ENTER to confirm No to resetting system parameters The instrument will return to the Select setup menu Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default YOU HAVE NOW COMPLETED THE INITIAL SETUP EXAMPLE As you learn more about the 2700 SELECT you will gain greater familiarity with the menu system If you would like to revisit levels of the Select setup menu press the appropriate number s If not press 0 to return to Select instrument function 2 23 2 24 Select instrument function 1 Service 2 Setup 3 Diagnostic Now press 0 again to return to Main menu Please select instrument mode RUN STANDBY MENU Now refer to Section 4 to learn about the recommended system parameters for your particular chemistry application Next use this information to setup the 2700 SELECT as we have demonstrated above HINT Use the Print Setup
148. ter calibrating with YSI standards are listed below Chemistry Membrane Calibration Standard FCN Limit Dextrose 2365 2776 2747 or 2355 0 05 g L Ethanol 2768 2790 2 00 g L 0 05 g L L Glutamate 2754 2755 0 06 g L L Lactate 2329 2776 2747 or 2327 0 03 g L Sucrose 2703 2780 0 10 g L Choline 2771 2772 0 02 g L L Glutamine 2735 2736 0 06 g L Methanol 2725 2726 1 00 g L 0 05 g L After a stable calibration with the recommended YSI calibration standard FCN readings greater than the limit may indicate membrane structural failure Recalibrate and repeat the FCN test If readings are still high refer to Section 8 Troubleshooting f you use units other than g L for the FCN test refer to Appendix A for conversion values Linearity Test Use the appropriate YSI linearity standard to test the linear range of the chemistry or chemistries you have chosen See the list below to be certain you are testing with the correct standard solution Place your instrument in RUN Mode When the unit is ready the following display will appear Ready to sample at Station 2 Pour a small amount of linearity standard into a test tube or cup and run it as a sample at either the Manual or Test Tube Holder Station NOTE If there is no test tube in the Test Tube Holder the sipper will automatically move to the Manual Station 3 for aspiration Acceptable linearity values for YSI standards are 5 See the list of acceptable values below
149. the 2700 SELECT YSI offers the Model 2730 Monitoring and Control Accessory for use in applications where monitoring and control is desired The Kit contains a peristaltic pump external sampling chamber and tubing for delivering a sample to the 2700 SELECT Purge time and sampling interval are selectable under control of the 2700 SELECT software Also included in this accessory are analog outputs representative of analyte concentrations The 2700 SELECT has been designed for future additions to allow even greater versatility related to sample handling and the measurement of additional analytes Therefore as accessories become available or new chemistries are developed you have a basic instrument upon which to build 1 2 STANDARD FEATURES e Single and dual chemistry configurations e Minimal sample preparation e Sample aspiration from a variety of tubes e Selectable sample aspiration volume 5 to 65 uL e Sample results in about one minute e Fluid sensing minimizes carry over e Fluid design washes inside and outside of sipper tube e Programmable automatic calibration e Selectable concentration units including g L mg L mmol L w v e Liquid crystal display and built in data printer RS 232 serial port e Turntable interface circuitry installed e User accessible service and diagnostic menus 1 3 1 3 1 4 General Specifications Sample size Response Time Power requirement Working enviro
150. time MTt Change monitor time interval MRt Change precal time interval MOt Change postcal time interval MSs Change monitor station PA Abort Turntable Sampling TNO Exit Run Mode Enter Standby Mode TNI Enter Run Mode from any mode Standby or Menu REPORT SYSTEM INFORMATION VO Report instrument model number VI Report software version number V2 Report software revision date RM Report system time date and measurement parameters RI Report instrument setup information 9 9 Common Commands The commands below can be invoked in either Remote Control Mode or in Result Reporting Mode unless otherwise stated Available commands are those in the Report Database and Report System Information categories above When Report Status RY is invoked the command obtains the 2700 status The status includes communications mode sample result status calibration result status machine status and remote command status Below is a summary of responses and the interpretations Host to 2700 RY 2700 to Host Communications mode R C Sample status result U N Calibrate status result U N Machine status I S C A M P N T F B K E H R Y D Remote command status I S C An example status line may appear as follows CNUII Report status of instrument Result reporting mode Remote control mode Unknown Results exist not sent to host No unsent results Last cal not sent to host No unsent calibration results Idle in Run Mode Proce
151. tion which can be determined by turning the equipment off and on the user is encouraged to try to correct the interference by one or more of the following measures e reorient the receiving antenna e relocate the computer with respect to the receiver e move the computer away from the receiver e plug the computer into a different outlet so that the computer and receiver are on different branch circuits If necessary the user should consult the dealer or an experienced radio television technician for additional suggestions The user may find the following booklet prepared by the Federal Communications Commission helpful How to Identify and Resolve Radio TV Interference Problems This booklet is available from the U S Government Printing Office Washington D C 20402 Stock No 0004 000 00345 4 E 2 Appendix F Warranty And Shipping Information The YSI Model 2700 Analyzer is warranted for one year from date of purchase by the end user against defects in materials and workmanship exclusive of batteries Within the warranty period YSI will repair or replace at its sole discretion free of charge any product that YSI determines to be covered by this warranty To exercise this warranty write or call your local YSI representative or contact YSI Customer Service in Yellow Springs Ohio Send the product and proof of purchase transportation prepaid to the Authorized Service Center selected by YSI Repair or replacement will be made and
152. tion unit other than g L the value automatically changes That is 0 50 g L becomes 5 62 mmol L or 500 mg L When non standard calibration values are used however the appropriate numbers must be entered whenever the measurement unit is changed ALWAYS VERIFY THAT THE CORRECT CALIBRATION VALUE IS ENTERED Use the information in Appendix A to make the necessary unit conversions for calibration entry Press ENTER to confirm and move to the next parameter Enter BLACK end point in seconds gt 30 Press ENTER to confirm the 30 second endpoint parameter The options range from 15 to 125 seconds The display now shows the Measurement parameter setup menu level Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White Press 4 for White Now set the same parameters for the White probe that you set for the Black probe Select WHITE chemistry Dextrose 0 Backup 1 Next chemistry ENTER Accept 2 19 2 20 Press ENTER to confirm Dextrose Select WHITE unit of measurement g L 1 mmol L 2 mg L ppm 3 g L 4 w v Press the appropriate number to change the default setting if necessary When the desired unit of measurement is selected press ENTER to confirm and move to the next parameter Enter White calibration value in g L gt 02 50 ALWAYS VERIFY THAT THE CORRECT CALIBRATION VALUE IS ENTERED Use the information in Appendix A to make the necessary unit conversions for calibration entry Press
153. tles are monitored for low levels and the waste bottle is monitored for high level The capacitive method depends on close proximity between the bottles and the metal chassis of the 2700 SELECT The bottles should always rest on the metal floor inside of the instrument It is also very important that the level sensor cables do not contact the metal chassis 6 9 Software Structure The 2700 SELECT has two operation modes RUN Mode where calibration and sample analysis occur and STANDBY Mode where system power is on but only a few maintenance functions occur This conserves reagents during extended periods of inactivity The 2700 SELECT also has menu selections for service setup and diagnostics The Main Menu of the software therefore provides three options RUN STANDBY and MENU Figure 6 5 shows you how to move from one mode to another and how to access the different functions within the menu selections and operating modes The lines of progress are marked with the name or number of the key you press to take that route Note that the RUN and STANDBY keys are toggle keys You may enter RUN Mode by pressing RUN and you may enter STANDBY Mode by pressing STANDBY You may switch back and forth from RUN Mode to STANDBY Mode by using these keys and confirming your choice by pressing ENTER To return to the Main Menu to have access to the full Menu options of Service Setup and Diagnostics you must exit RUN Mode or STANDBY Mode by pr
154. traction of a large dextrose signal from a smaller sucrose signal may result in significant errors in the sucrose reading An alternative method involves using two different and appropriate dilutions of the sample to first measure free dextrose then treat one sample with invertase and analyze for dextrose liberated from the sucrose Contact YSI Customer Service or your dealer representative for an application note explaining this approach in detail 4 19 4 18 4 20 Simultaneous L Glutamate and L Glutamine You must have a dual channel version of the 2700 SELECT in order to perform this measurement Refer to the previous sections on L GLUTAMATE and L GLUTAMINE for theoretical and special considerations then follow the instructions below to set up your instrument for simultaneous determination Since the Glutamine Membrane 2735 utilizes a chain of enzyme reactions and the final reaction is a glutamate measurement free glutamate in a sample will be measured at the Glutamine Sensor By using the information at the Glutamate Sensor this free glutamate is automatically subtracted in the instruments software The algorithm looks basically like this Total Glutamate Free Glutamate Glutamine Total Glutamate is the combined glutamine and glutamate probe signal Free Glutamate is the glutamate not derived from Glutamine reaction System Buffer YSI 2357 Calibrator Std YSI 2755 and YSI 2736 Linearity Std Y
155. trate the flexibility you have with the 2700 SELECT For illustration purposes we will demonstrate initial setup for simultaneous determination of dextrose and L lactate on a dual channel 2700 SELECT NOTE If you have a single channel unit this example has more information than you need However it will cover all pertinent information to set up a single channel unit This is a straight forward setup utilizing one calibration solution to perform simultaneous chemistries Follow along by pressing keys on your 2700 SELECT This exercise will be a good learning tool After completing the exercise you will be directed to parts of the manual related to your specific chemistry interests EXAMPLE Set up the YSI 2700 SELECT to measure D glucose dextrose and L lactate simultaneously Let us assume you have installed a YSI 2329 Lactate Membrane on the black probe and a YSI 2365 Glucose Membrane on the white probe YSI 2357 buffer has been reconstituted and poured into the Buffer Bottle and YSI 2776 Standard 2 50 g L dextrose and 0 50 g L L lactate has been installed into the Calibrator Bottle position The instrument has been powered and the printer paper installed Let us also assume that you have your sample in a test tube and want to program the unit to run the sample three times in succession triplicate You would also like to identify the sample by an identification number to be printed with the result To conserve printer paper you would like
156. trument and then into the power mains Refer to Section 2 5 Power Up to confirm correct power up response 7 10 Sipper Pump Seal Replacement Replace the Sipper Pump seals every 6 months Heavy usage may warrant more frequent replacement Disconnect the tubings to the Sipper Pump Remove the Sipper Pump head from the instrument wall see Figure 7 5 TO SIPPER TUBE ey d SIPPER PUMP NS HEAD ASS Y e o NS Figure 7 6 Sipper Pump Head Removal Pull the white pump base from the clear pump housing If salt deposits have formed on the parts wash them off with warm water Replace the seals as shown in Figure 7 6 Extra seals are supplied in the Preventive Maintenance Kit 7 11 Figure 7 7 Sipper Pump Seal Replacement Reassemble the pump position the plunger as shown in figure 7 7 and install it back on the instrument Figure 7 8 Sipper Pump Plunger Position WARNING When re installing the pump head assembly the plunger MUST extend at least 1 2 from the base of the pump see Figure 7 7 This will assure proper alignment between the pump head and the drive hub 7 11 Sipper Mechanism Lubrication The sipper mechanism needs only occasional cleaning and lubrication If the acme screw and guide rods are reasonably clean and have a thin film of lubricant on them no further maintenance is required If the screw or either rod has a dirt build up or if the mechanism squeaks or jams the
157. uffer calibrator and waste bottles One reason to inactivate the level sensors would be if you use a non standard container for buffer or waste Activate bottle level sensors Yes 1 No 2 Yes Press ENTER to confirm and return to General setup level General setup 1 Date Time 2 Contrast 3 RS232 4 Radix 5 Serial 6 LevelSensor You have now completed General setup Press 0 to return to Select setup menu level Select setup 1 General 2 MeasParameter 3 RunMode 4 Report 5 PrntSetup 6 Default Press 2 for MeasParameter The display will read Measurement parameter setup 1 SampleSize 2 CalMethod 3 Black 4 White MeasParameter is an abbreviation for measurement parameters In this menu you may select the sample size and assign the location of the calibrator solution s You will also assign specific chemistry parameters to the Black probe single channel or Black and White probes dual channel These parameters include chemistry assignment calibration value units of concentration and time to endpoint of the enzyme sensor reading NOTE YSI has assigned default values for each of these parameters but provides flexibility for the user to change values to optimize for a particular application Press 1 for SampleSize The default setting is 25 microliters The range is 5 to 65 Enter sample size in microliter gt 25 Press ENTER to confirm and return to Measurement parameter setup Measurement p
158. ur unit of choice If you change units remember to enter the appropriate numerical value when prompted by the display Only the default values listed above will be automatically converted by the instrument software A 1 Example Conversions Beginning with 2 50 g L dextrose convert this to mg L then and finally to mmol L 1 Multiply by unit conversion s 2 Cancel units common in numerator and denominator 3 Multiply numbers mg L 2 50 g L 2 50 g L 1000 mg g 2 50 1000 mg L 2500 mg L w v 2 50 g L 2 50 g L 0 1 L dL 2 50 0 1 g dL 0 250 g dL 0 250 Note g dL is g 100ml or percent mmol L 2 50 g L 2 50 g L 1 mole 180 g 1000 mmole mole 2 50 1 180 1000 mmole L 13 89 mmol L Linearity Test Concentration Unit Conversion mg L mw Chemistry g L ppm w v mmol L g mole 0 47 473 0 047 4 54 Choline 0 45 450 0 045 4 32 104 0 43 427 0 043 4 10 9 45 9 450 0 945 52 5 Dextrose 9 00 9 000 0 900 50 0 180 8 55 8 550 0 855 47 5 3 36 3 360 0 34 73 1 Ethanol 3 20 3 200 0 32 69 6 46 3 04 3 040 0 30 66 1 1 54 1 535 0 154 9 5 L Glutamate 1 46 1 462 0 146 10 0 146 1 39 1 389 0 139 10 5 1 23 1 228 0 123 8 40 L Glutamine 1 17 1 169 0 117 8 00 146 1 11 1 111 0 111 7 60 2 80 2 806 0 281 31 5 L Lactate 2 67 2 672 0 267 30 0 89 2 54 2 539 0 254 28 5 26 25 26 250 2 63 76 8 Lactose 25 00 25 000 2 50 73 1 342 23 75 23 750 2 38 69 4 2 63 2625 0 263 82 0 M
159. ut output devices such as display keypad and printer for proper function And finally you can check the status of most sensors in the system These include the temperature sensor in the Sample Chamber the level sensors in the reagent and waste bottles the Sipper capacitance sensing and the optical sensors that control motors the Sipper Pump and sense the Test Tube Holder position Follow the display prompts and information below to learn about the 2700 SELECT menu diagnostics mode As before you will often need to press 0 to return to a previous menu level Occasionally the instrument will print diagnostic information Refer to Figure 5 3 below to become familiar with major components of the instrument Additional figures in Sections 3 and 5 may be useful especially Figure 5 2 Menu Flow Chart 7 WD 2 BUFFER Figure 5 3 2700 SELECT Major Components 5 17 As discussed in the Introduction to this section you must exit RUN Mode or STANDBY Mode to gain complete access to the diagnostic menu functions To exit Run or Standby modes press the appropriate key RUN or STANDBY then follow the prompts to exit You will now be in Main Menu mode and the display will appear as shown below Please select instrument mode RUN STANDBY MENU Press MENU then 3 for Diagnosti
160. utamine reading Since the glutamine membrane contains glutamate oxidase glutamate will produce a probe signal See Section 4 17 to measure glutamate and glutamine simultaneously 4 15 4 15 Methanol This is a direct reading of Methanol in solution at the enzyme sensor The enzyme Alcohol Oxidase is immobilized in the enzyme membrane Methanol H O Formaldehyde Alcohol Oxidase System Buffer YSI 1579 Calibrator Std YSI 2726 Solution A 1 00 g L Linearity Std YSI 2726 Solution B 2 50 g L Black Probe YSI 2725 Methanol Membrane White Probe n a Sample Size 15 uL for best linearity Cal Station Station 42 Test Tube Holder Black Probe Parameters Chemistry Methanol Unit of Conc g L Cal value 1 00 g L End Point 30 sec f you have a dual channel unit either Black or White or both Black and White probes can be configured for this chemistry You may use Station 1 Cal Well but evaporation through plastic components may be a problem See the evaporation control information in special considerations Note See Appendix A if concentration unit conversion is required Special Considerations The linear range of methanol is quite limited If you are concerned about linearity monitor the upper range of concentration on a regular basis You may benefit by preparing and using a calibrator with a methanol concentration close to your sample concentration If you prepare your own methano
161. ve shield ground Transmit data put Recieve data utput Request to send put Clear to send Input Data set ready Signal return ground aput Data carrier detect utput Data terminal ready GPO 1A 6 Owe Co Ge li Tt aoe ZA ES UA WO E E 22 ju un EG Figure 9 1 RS 232 Signal Description and Direction 9 1 9 2 Hardware Handshaking Signals The four handshaking signals used to control the data flow are RTS CTS and DTR DCD The 2700 drives RTS when it wishes to transmit and waits for CTS before transmitting each character Likewise the 2700 asserts DTR when it is ready to receive data and receives only when DCD is asserted DSR is not used Software Handshaking Signals In the XON XOFF handshaking protocol RTS and DTR are always asserted and CTS and DCD are always ignored Since the 2700 acts as a DTE it requires a shielded NULL modem cable to connect to another DTE such as the PC Figure 9 2 and Figure 9 3 show possible NULL modem cable connections Figure 9 2 Full Handshaking DTE Interface 2700 MALE Figure 9 3 Three Wire DTE Interface You can select the 2700 handshaking protocol RTS CTS or XON XOFF In addition you can select baud rate data length bits per character parity stop bit and configuration multipoint or point to point For information on communications setup see Section 4 2 Menu Setup The communi
162. w and jumps This jumping action helps clear the sample chamber of air bubbles during a flush cycle Adjust until stir bar jumps 1 Increase speed 2 Decrease speed Using the 1 Increase and 2 Decrease choices adjust the Stir Bar Lower the speed to observe synchronous rotation then increase the speed until the stir bar jumps and looses synchronous rotation At this point release the 1 key If jumping does not occur adjust the Stir Bar speed to the maximum setting Press 0 to exit the stir bar menu level Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable CAUTION Do not enter the 5 Monitor or 6 Turntable submenus unless the monitor or turntable is installed AND the left access plate is removed You may seriously damage the sipper arm assembly If you press 5 for Monitor you will access the functions used to align and prime the YSI 2730 Monitor and Control Accessory Refer to the YSI 2730 Monitor and Control Accessory User s Manual for details If you press 6 for Turntable you will access the functions used to align the YSI 2710 Turntable Refer to the YSI 2710 Turntable Operation Manual for details Press 0 two times to backup to the instrument function selections Select instrument function 1 Service 2 Setup 3 Diagnostic You have now completed the Service menu selections 5 3 Setup Selections Setup menu is where you will enter system parameters that define the specific informati
163. y The Waste will show OK or Full Level sensor status Sipper Fluid Cal OK Waste OK Buffer Empty Press 0 to return to Sensor diagnostic menu Sensor diagnostic 1 Temperature 2 LevelSensor 3 OptoSensor Press 3 for OptoSensor Optosensors are used to track horizontal and vertical movement of the Sipper the movement of the Sipper Pump plunger and the presence of a test tube in the Test Tube Holder Opto sensor status Horiz OFF Vert ON SipperPump ON TestTube ON If the Sipper is positioned at Station 2 or 3 the Horiz message should be OFF if at home or Station 1 the message should be ON If the Sipper is down in the Sample Chamber the Vert message should be OFF if up the message should be ON If the Sipper Pump plunger is at home i e extended the SipperPump message should be ON if the plunger is in any position other than home the message should be OFF If there is a test tube in the Test Tube Holder and it is in a vertical orientation the TestTube message should be ON if not then the message should be OFF Press 0 to return to Sensor diagnostic menu Sensor diagnostic 1 Temperature 2 LevelSensor 3 OptoSensor Press 0 to return to the five diagnostic selections Select diagnostic 1 Motor 2 Pump 3 Probe 4 I O 5 Sensor Press 0 to return to Select instrument function Select instrument function 1 Service 2 Setup 3 Diagnostic Press 0 to return to Main Menu di
164. y messages will guide you through the procedure It is very important that the Sipper be accurately adjusted Adjust sipper then select 0 Exit 1 Lower sipper for fine alignment Fine align sipper then select 0 Exit 1 Test sipper position Select 1 to restart check sipper cycle 0 Exit 1 Home sipper position Adjust sipper then select 0 Exit 1 Lower sipper for fine alignment When completed press 0 for Exit to return to service selections Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable 5 4 Press 2 for Buffer to initiate a 15 second cycle of the Buffer Pump This selection allows you to prime the buffer system Use it after replenishing the buffer bottle or replacing tubing Also use it to troubleshoot problems or find leaks Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable Press 3 for Cal to initiate a 10 second cycle of the Cal Pump This selection allows you to prime the calibrator system Use it after changing the calibration solution or replacing tubing Also use it to troubleshoot problems or find leaks Select service 1 Sipper 2 Buffer 3 Cal 4 Stir speed 5 Monitor 6 Turntable Press 4 for Stir Bar to adjust the stir bar speed During RUN Mode operation the stir bar has two operating speeds normal speed at which the stir bar rotates smoothly in the chamber and accelerated speed at which the stir bar looses synchronization with the motor housed belo
165. zed and periodically freshens the buffer to the sample chamber When entering RUN Mode the unit will automatically update its calibration as required In MENU Mode there are three submenu levels Service Setup and Diagnostics Notice that you may move from RUN Mode or STANDBY Mode to limited menus in Setup However to fully access MENU Mode you must exit RUN or STANDBY modes Please select instrument mode RUN STANDBY MENU STANDBY STANDBY STANDBY STANDBY MODE RUN i CALIBRATIUN Figure 5 1 2700 SELECT Software Structure To exit RUN Mode press RUN then confirm your exit by pressing 2 for Yes then ENTER The instrument will next display the message shown below To exit STANDBY Mode press STANDBY then confirm your exit by pressing 2 for Yes then ENTER The instrument will also display the message shown below The display message shown below is referred to as Main Menu display This is where we will begin Please select instrument mode RUN STANDBY MENU 5 1 5 2 Please select instrument mode RUN STANDBY MENU uffer pu Cal purnp xt pump ush librator Sample L Ext Purmo SampleP toco Ext Chamber AutoStandby

YSI 2700 SELECT User`s Manual

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