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Operating Manual AutoTEC (ENG)

1. z es SIS E o Qa aa aa aaa oro oral ole Ga cad Ge ca FE Eg d 0 Pls Poli ny mi on TOTAL CASSETTES 8 WARM UP 1 27 2010 7 00 00 gt CASSETTES TO THE CHUTE 0 1 26 2010 10 00 00 a RESERVOIR TEMPERATURE 64 13 C PARAFFIN LEVEL GOOD Figure 3 F 3 4 Displays information on the cassettes ran based the following selected criteria e Date ranges e Today This Week Last Week or this month File Output button When selected will save selected run history to an external memory stick Expand All Columns button When selected toggles the display to show all columns in one screen or to show all information inside each column requires user to use the horizontal scroll bar to see all displayed information SERVICE Screen Only available with Service level login SERVICE screen is displayed when the SERVICE tab is selected Figure 3 G Jan 26 2010 Sakura R amp D LaB Ay Tissue Tek AutoTEC 114025 am Es NOTE USING THE SETUP UTILITIES WITHOUT EXTENSIVE KNOWLEDGE OF THE INSTRUMENT CAN RESULT IN DAMAGE TO THE INSTRUMENT BE CAREFUL USING THESE UTILITIES Si P HEA
2. TOTAL CASSETTES WARM UP 3 10 2010 6 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 3 10 2010 8 00 00 PM RESERVOIR TEMPERATURE 63 8 C PARAFFIN LEVEL GOOD Figure 3 U Languages Setup screen 3 11 CUSTOMIZATION OF SETTINGS Once a language selection is made from the drop down list the System Setup screen will be displayed in the selected language immediately Figure 3 V However the selection is not saved until the SAVE AND EXIT button is pressed a 10 2010 SakuraFINETEK 3 Tissue Tek AutoTEC 1 HISTOR DE J PROCESOS LINGUA italiano nglis Francais VOLUMEN DE ALARMA Deutsch ALLARME Es spafiol SONIDO DE ALARMA ederlands S FINE TEST RIPRISTINA VALORI PREDEFINITI suomi SONIDO DE FIN DE PROCESO Dans P ors FECHA Y HORA Cestina ISTITUZIONE SakuraFINETEK SALVA E MODIFICA ESCI ESGI TOTAL CASSETTES CALENTAMIENTO 3 11 2010 7 00 00 CASSETTES ALA RAMPA 0 ENFRIAMIENTO 3 10 2010 7 00 00 PM TEMPERATURA DEL DEP SITO 63 64 C NIVEL DE PARAFINA BIEN Figure 3 V System Setup screen in selected language The selected language will be used in all other screens It will take a few seconds for all the screens to be updated Figure 3 W Mar 10 2010 SakuraFINETEK i AutoTEC a E
3. fd ee beu toss 1 5 Output grid acutus 52 3 tatt e 1 1 Paraffin reservoir 1 5 Paraffin reservoir filter 5 2 Paraform recovery 1 4 2 2 Robotic system aka 1 3 Stager 1 4 Configuration System Settings 3 7 Consumable S Uo odisea tot 7 2 Contact 2 2 1 7 1 Embedding Tracking CODES itt Pardi n ose a 4 5 4 5 Environmental FACING d dee eti m 2 1 255 54 seduces an 3 15 Error recovery Revised 5 19 2010 Appendix B Error code NOES E RE 6 4 Error codes and 6 7 I ROCGOURO or dtt td bet d LED LE 6 7 UR 3 14 G HI 9055 LE 1 6 SUITE Stat lt 4 1 ER 1 3 Installation 21 5 0 6 7
4. TX mama ma fm vv Beano pore or wesma mama 0 Proto 1 51 mamm Figure 1 E Interior View of Robotic System As shown in Figure 1 E the robotic system comprises two main modules a gripper and a stager each with three axes of motion These two modules handle the Paraform Cassettes from the magazine area the base mold module and finally to the output door The gripper retrieves Paraform Cassettes from the magazines and moves them through the system Figure 1 F shows the cassette sensor RJ wide beam sensor M and beneath it the Paraform recovery chute PI with chute sensor The sensors detect an open lid presence and type of cassette they also allow the system to determine if the frame is correctly held by the gripper If a tissue cassette is not present improperly assembled or if the frame is incorrectly held the gripper drops the item into the Paraform recovery chute If the chute sensor detects an item is dropped into the chute the system indicates that a cassette was dropped in the chute and the CASSETTES SENT TO CHUTE count wil be incremented on display The gripper is programmed to attempt to pick a framed cassette from a magazine in three attempts After the third attempt to pick up a cassette if the gripper still does not grab a frame from the magazine the instrument will retry for 3 more times after cycling the ID motor up and down to sort out the stacks
5. 3 Revised 5 19 2010 After a successful log in display screens can be changed by selecting the tab for each screen and buttons are enabled to allow the initiation of the embedding process MONITOR Screen Monitor screen is displayed when the MONITOR tab is selected Figure 3 C rum R amp D 2 7 29 2010 Tissue Tek AutoTEC UTILITIES ERRORLOG OVERRIDE IHSTRUMENT LOAD TOTAL CASSETTES WARM UP 1 30 2010 7 00 00 AM 2 CASSETTES TO THE CHUTE 0 COOL DOWN 1 30 2010 10 00 00 a RESERVOIR TEMPERATURE 64 38 C PARAFFIN LEVEL GOOD Figure 3 C Time and date are displayed at the top center of the screen Institution name and the current logged in user name are displayed at the top right corner of the screen to allow screen changes between MONITOR UTILITIES ERROR LOG and RUN HISTORY There is a SERVICE mode which is only shown with Service level log in Graphical representation of Input Doors and Output Doors area There are individual counters for the number of cassettes in the Input Doors and the number of embedded blocks in the Output Doors Graphical representation of the Base Mold area with individual counters for each of the 6 cassette types Standard Biopsy Orientation Core Biopsy Shaved Biopsy and 13x13 3 1 CUSTOMIZATION OF SETTINGS Button Descriptions STOP When selected it pauses the current instrum
6. als amp lissue uto 7 SAKURAN Kitt Automated Tissue Embedding Console 2010 Sakura Finetek USA Inc All Rights Reserved Printed in U S A Manufactured by als Sakura Finetek USA Inc 0 Torrance 90501 U S A SAKURA Made in U S A 0003055 02 Rev E TABLE OF CONTENTS Section Page 1 INTRODUCTION Safety Precaution S sosina titulus iens ie uen 1 1 General DES CHIP OM iter 1 1 Location of Major Systems and Components e esee eret retener tentes ttnn tentent 1 1 Rem a 1 6 Sall SEA das iecore edits E I LN ne E IM 1 7 2 INSTALLATION General MALOV AA scri ient p iet isa di no baee 2 1 Environmental 3 85 AE 21 Unpacking and OTI acude lol 2 1 toa 2 2 3 CUSTOMIZATION OF SETTINGS General OM cn ene del un 3 1 Interface SGEeGTIS ascen e NE UR 3 5 Access T P M 3 6 Conlhgurng System 224 4 OPERATION dee 4 1 Typical Operating SEG UCI Ce
7. 10 0 15A 60 2 20 7001 100 VAC 10 5 lt 15 50 60 Hz 20 7002 230 VAC 10 lt 7A 50 Hz 10A We recommend an uninterruptible power supply 7011 or 7146 or at least a dedicated line with an appropriately rated line conditioner Noise Level Less than 65 db Environmental Requirements Operating Temperature Range 15 C to 35 C WARNING IF TEMPERATURE REACHES 36 OR HIGHER EMBEDDING WILL STOP UNTIL AMBIENT TEMPERATURE IS LOWERED Nominal Temperature 22 C 2 C Relative Humidity 20 to 85 non condensing Nominal Humidity 40 20 Relative Atmospheric Pressure 70 to 106 kPa Storage Temperature Range 10 C to 65 C Relative Humidity 20 to 95 non condensing Relative Atmospheric Pressure 70 to 106 kPa Revised 5 19 2010 Dimensions and Weight Height 154 cm 60 6 in Width 136 cm 53 7 in Depth 76 cm 29 9 in Weight 386 kg 850 Ibs Instrument Life Expectancy 7 years based on eight hours of operation five days a week with a maximum of 960 cassettes per day if the operating and instrument care instructions found in this manual are followed and if preventative maintenance is performed at least once every year by a qualified service technician Safety Standards Electrical Safety The Tissue Tek9 Auto TEC has been tested and it complies with the following standards e 61010A 1 e UL 61010A 2 010 e 5 22 2 1010 1 92 e 5 22 2 1010 2 010 R
8. 2 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 PM a RESERVOIR TEMPERATURE 321 C PARAFFIN LEVEL GOOD Figure 3 E Up to date accumulated error messages can be sorted by date ranges error code ID and User name Total number of errors in the sorted log SAVE button When selected will save sorted error log to an external memory stick RUN HISTORY Screen RUN HISTORY screen is displayed when the RUN HISTORY tab is selected Figure 3 F Jan 25 2010 Sakura R amp D LaB Bh Tissue Tek AutoTEC TUESDAY 11 34 02 AM 7 UTILITIES ERROR OG E Summary erg BM 1 19 2010 Pg ype Severa is 2 Expand All File Output SURE qus ove 22 es Am doc ace a ey oe E 5 5541 ke gt ese esel m i ime inne ime ime imt imt imi em em em 0 0 jo o 0 o F 0 1 7 0 1 sg imt ae Magazine 9 Magazine2 0 Magazine3 D 4 0 m m a 2 gt v v 1 1 1 1 0 1 1 9 9 lt 4 al lt 0 1 0 8 lt k p lt 1 2 3 5 8 1 2
9. ET 4 1 Bote eene T E e 3 1 Map of interface 3 5 MONOT sorasa c EM EE 3 1 Password nl Vs isses audit 3 14 4 1 Reservoir Temperature 4 2 RUM RISO 3 4 RUN SCREEN S esten dct rate d E 4 2 4 5 TEO HP n 34 3 9 Software Version 3 10 slem Sepia sarete 3 11 LEMS E I Ee 33 Warming Cassettes 4 4 SOPVIGE fA SONWALE celia a 3 3 T iFeennicalsupDOl 7 1 Temperature conditions esee 1 6 Touch Screen control 1 6 B 2 UX Unpacking and Handling eee 2 1 User icons PACU esses 3 6 STAC cal sas Rn 3 6 Revised 5 19 2010 Revised 5 19 2010 D 1 D 2 Revised 5 19 2010 Revised 08 01 2005 B 3
10. EXIT TOTAL CASSETTES WARM UP 3 10 2010 6 00 00 2 CASSETTES TO THE CHUTE 0 COOL DOWN 3 10 2010 8 00 00 PM RESERVOIR TEMPERATURE 64 68 C PARAFFIN LEVEL GOOD Figure 3 Z System Setup screen Time Select SAVE AND EXIT to save the changes Select EXIT to abort the changes NOTE Time can only be changed when AutoTEC is idling and there is no timer running such as the 4 Hr warming up timer Revised 5 19 2010 Section 3 INSTITUTION Setup Select EDIT to bring up CHANGE INSTITUTION keyboard Figure 3 Use BACKSPACE to clear current entry Use keypads to enter new name SHIFT to change letter fonts Select to ENTER to confirm and save current changes The new institution name will be updated immediately and can be seen at the upper right corner of the LCD display Select EXIT to return to UTILITIES screen Feb 11 2010 Sakura R amp D Lab i K Tissue Tek AntoTEC tuna e CHANGE INSTITUTION NAME Sakura R amp D Lab 3 PEELE EE EEL 0 TOTAL CASSETTES 0 WARM UP NO TIME SET 2 CASSETTES TO THE CHUTE 0 COOL DOWN TIME SET a RESERVOIR TEMPERATURE 0 C PARAFFIN LEVEL GOOD Figure 3 AA System Setup Keyboard 3 19 CUSTOMIZATION OF SETTINGS User Accounts From the Utilities screen Figure 3 BB touch the USER ACCOUNTS icon to bring up MANAGE USER ACCOUNTS screen A total of up to 13 Administrators and 4
11. Figure 1 B Rear View and Right Panel Figure 1 C Main Door Opened Revised 5 19 2010 Input Door and Loading Area Figure 1 D Figure 1 D Input Door The input door is accessed by opening the main door see Figures 1 and 1 C The input door is interlocked enabling the operator to insert and remove magazines only when the robotic system is not interacting with the door An insulated housing enclosure ensures efficient magazine heating Precise positioning of each cassette ensures accurate placement in the base mold The input door includes four individually controlled heater assemblies see Figure 1 0 location that Accommodate one magazine each e Heat magazines in 15 minutes to prepare cassettes for embedding e Present Paraform Cassettes consecutively to the gripper NOTE The input door cannot be opened during operation unless an on screen prompt indicates otherwise Access to the robotic system is through the two rear doors see Figure 1 B location EA NOTE Before opening the rear doors or the front door the Instrument Access button on INSTRUMENT the monitor screen must be pressed ACCESS first Wait for the AutoTEC to stop the current embedding cycle and to display an acknowledgement prompt on the LCD screen before opening the doors Opening the door without waiting for the prompt can cause robotic system stalls and will delay the normal embedding cycles Revised 5 19 2010 Section 1
12. From To 2 7 2010 2 13 2010 Summa 4 FH BM oolin 3lock 00 0 00 2vere cove Start Time Time Time Time Time Time Time 2 8 2010 E 2 8 2010 i Magazine1 4 Magazine2 4 Magazine3 12 Magazine5 1 i ls Magazineb 1 2 10 2010 2 12 2010 TOTAL CASSETTES WARM UP 2 15 2010 8 00 00 AM CASSETTES TO THE CHUTE 0 COOL DOWN 2 15 2010 10 00 00 RESERVDIR TEMPERATURE 64 27 C PARAFFIN LEVEL GOOD Figure 3 00 One magazine view 3 17 CUSTOMIZATION OF SETTINGS Displayed columns can be toggled between expanded or compressed to fit the LCD display area by selecting the Expand Columns button Figure 3 PP Feb11 2010 Sakura RAD Lab AutoTEC THURSDAY 08 11 13 AM DMIN From 2 7 2010 mm J Ej Summary E Total Total Total Total Total Total t Load des 5 2 7 2010 Seid YCE sssette STD BX 3pecia 13x13 Others 299 Time Time 2 8 2010 9 2 0 Br 2 072010 Magazine 2 Magazine2 0 Magazine3 D 4 1 TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 RESERVOIR TEMPERATURE 277C PARAFFIN LEVEL GOOD Figure 3 Expand view The run history display can be rearranged by selecti
13. Grossing Procedures for Paraform Sectionable Cassettes sss C 1 APPENDIX D Microtomy Procedure for Paraform Sectionable Cassettes sss D 1 i Revised 5 19 2010 INTRODUCTION Safety Precautions WARNINGS CAUTIONS and NOTES are provided throughout this manual to indicate levels of potential hazards as defined below WARNING Identifies a potential hazard where failure to follow instructions may result in serious injury or death to the operator and or other personnel CAUTION Indicates a potential hazard where failure to follow instruction may result in damage to the Tissue Tek AutoTEC and or other property NOTE Indicates a reminder or other helpful information General Description The Tissue Tek AutoTEC is a reliable high quality precision instrument that fully automates the embedding process using the innovative Tissue Tek Paraform Sectionable Cassette System The Tissue Tek AutoTEC embeds diagnostic human and research tissue specimens continuously enabling laboratories to e Significantly improve throughput time Enhance procedure standardization and specimen consistency Reduce repetitive movement disorders and exposure to harmful chemicals e Offer a continuous method of tissue specimen embedding that eliminates the need to handle samples in batches e Automatically embed up to 120 specimens per hour which improves productivity e Enhance
14. LUZ CARGAR ENCENDIDA SALIR TOTAL CASSETTES CALENTAMIENTO 3 11 2010 7 00 00 CASSETTES ALA RAMPS 0 ENFRIAMIENTO 3 10 2010 7 00 00 PM TEMPERATURA DEL DEP SITO 63 7 C NIVEL DE PARAFINA BIEN Figure 3 W Screen Updated in Selected Language 3 12 Audible Alarm Setup As shown in Figure 3 X System Setup screen there are three alarm attributes that can be modified Select between three volume levels High Middle Low eight different alarm sounds labeled as Alarm 1 though Alarm 8 and eight different end of process sounds labeled as End Process 1 through End Process 8 Select the down arrow to activate the pull down list then scroll to highlight the selection The following screen shows how to set Alarm volume to LOW Mar 10 2010 Sakura R amp D LaB ox 2 22 Tissue Tek AutoTEC wepxespay 091418 AM ERROR IOC LANGUAGE English 7 ALARM VOLUME TEST ALARM HIGH ALARM SOUND TEST END END PROCESS SOUND End Of Processi RESTORE DEFAULTS DATE AND TIME Tuesday March 09 2010 5 29 18 PM 4 INSTITUTION Sakura R amp D LaB SAVE AND TOTAL CASSETTES WARM UP 3 10 2010 6 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 3 10 2010 8 00 00 PM RESERVOIR TEMPERATURE 63 73 C PARAFFIN LEVEL GOOD Figure 3 X System Setup screen Alarm Volume Select the TEST ALARM or TEST END PROC button will output an audible
15. Low Profile Disposable Blades product code 7089 10 Dispensers x 50 blades cs e issue Tek9 Paraform Sectionable Cassettes 500 cs e T issue Tek Cleaning Foam Swabs 25 cs product code 8050 Cassette Type Product Code 7019 Ancillary Products Biopsy 26 x19mm 7020 Standard 7021 Ancillary products for the Tissue Tek Auto TEC include Orientation 7022 e issue Tek Accu Edge Grossing Board Standard Shaved Biopsy 7023 product code 4800 Core Biopsy 1024 e issue Tek Paraform9 Frames Frames are also available stacked for compatibility with the Tissue Tek9 Autowrite Cassette printer 500 case Stacked 10x40 cs Frame Color Product Code Product Code Agua 7030 7070 Blue 7031 7071 Gray 7032 7072 707 ley Tissue Tek Accu Edge Grossing Board Large dia ae oa product code 4801 B eis e e Tissue Tek Accu Edge Grossing Wells product in 2 n code 4802 s es e Tissue Tek Accu Edge Grossing Fork 1 5 mm i product code 4803 200 e Tissue Tek Accu Edge Grossing Fork 2 0 mm ite product code 4804 Yellow 7041 7081 Tissue Tek Accu Edge Grossing Fork 2 5 mm product code 4807 T 2 Revised 5 19 2010 Section 7 How to Order Replacement Parts In the United States replacement parts can be ordered directly from Order Management Sakura Finetek U S A Inc 1750 W 214th Street Torrance CA 90501 or by calling toll free 1
16. blocks Prepare for the next run End of operations e Review Reports e Perform maintenance as required Cool Down 4 1 Routine Operation Warm Up The Tissue Tek AutoTEC requires a four hour warm up time at initial start up or if it was cooled down The warm up period can be programmed to start automatically at a set time Following warm up the Tissue Tek AutoTEC is in Operating mode ready to embed A valid user must be logged in to start embedding Preliminary Checks Remove then either clean or replace the paraffin trough Figure 1 C Location 12 Place trough back into position before beginning operation Ensure that paraffin levels are adequate prior to starting operations When adding paraffin to the reservoir during normal hours of operation use molten paraffin do not overfill the reservoir Use the paraffin fill gauge shown in Figure 1 J as a guide At the end of the work day paraffin pellets may be added Only Tissue Tek Paraform Sectionable Cassettes and Tissue Tek Paraform Processing Embedding Medium Formula 3 product code 7052 can be used in the Auto TEC See Section 7 Service and Replacement Parts for information about how to order cassettes and additional paraffin Confirm that the output doors are empty and that the front and rear access doors are closed and latched Depending on the initial thermal state of the system and the room temperature all internal c
17. frame and cassette will be placed in the cassette recovery chute The chute is located behind a spring loaded hinged door on the left side panel The system will continue to process cassettes and prompt the operator to remove the Paraform Cassette from the chute Touch to confirm that the cassette has been removed The gripper is programmed to attempt to pick a framed cassette from a magazine in three attempts The gripper may miss the cassette on the first attempt but move to the chute and then return for another attempt which is successful If the cassette type check sensor validation fails for the paraform cassette coming from the magazine in the input door the gripper will place that cassette in the chute and return to the input door to grab the next cassette CASSETTES SENT TO CHUTE count on screen will be incremented each time the chute sensors detects cassette frame dropping to the chute Figure 4 N Jan 28 2010 Sakura R amp D Lab Tissue Tek AutoTEC CASSETTES TO THE CHUTE 1 COOL DOWN 1 28 2010 5 15 00 PM RESERVOIR TEMPERATURE TOTAL CASSETTES WARM UP 1 29 2010 1 45 00 PM 2 a 64 52 C PARAFFIN LEVEL GOOD Figure 4 N Cassette Recovery Message 4 7 OPERATION 4 8 Revised 5 19 2010 Section 5 CARE OF THE INSTRUMENT Introduction This section provides information on how to care for and maintain the Tissue Tek AutoTEC to keep it in good condition and to ensure proper operation Pro
18. seconds RESUME ABORT Figure 5 Q To clean the gripper arms select ABORT on the above message box If the time is exceeded the system will resume current operation in 180 seconds To override cleaning at this time select RESUME to let the instrument continue with the current task If operator selects ABORT to clean the gripper arms then the stager will move over standard base mold 1 and gripper will move above base mold 5 giving enough clearance to clean the bottom of the gripper arms Once in the position wait for the following message to be displayed on the screen before attempting to open the door Figure 5 R Please open the front door and carefully wipe off any paraffin drops or accumulation from the bottom of the gripper arms When done please close front door and touch OK to continue Figure 5 R Revised 5 19 2010 Section 5 Open the front door to clean the gripper arms Figure 5 S and follow Gripper Arms Cleaning Procedure located on the previous page Figure 5 S After cleaning the gripper close the front door to allow the instrument to resume operation After closing the front door acknowledge message box on screen to continue with the cycle Figure 5 R NOTE During an embedding cycle if Gripper Parallel Check Failure occurs there will be NO request to clean the gripper arms since they were successfully checked and passed before starting the cycle 5 7 CARE OF THE INSTRUMENT 5
19. 1999 e 61010 1 e 61010 2 010 e EMC EN 61326 1998 compliance to directive 98 79 EC Regulatory Information FDA registered with ETL evaluation USA complies with requirements of The Pharmaceutical Affaires Law Japan CE registered in compliance with the requirements of EMC Directive LV Directive in Europe Europe In Vitro Diagnostic Device Directive California Building Code Fixed Equipment Anchorage Pre Approval 2001 OPA 1866 2007 OPA 1866 07 Revised 5 19 2010 Section 1 INTRODUCTION 1 8 Revised 5 19 2010 INSTALLATION General Information This section provides information on determining a location for and installing the Tissue Tek AutoTEC The Tissue Tek AutoTEC must be located and installed correctly to ensure proper operation and service Read this operating manual carefully before attempting to operate the Tissue Tek AutoTEC instrument Follow all instructions carefully CAUTION The Tissue Tek AutoTEC should be installed adjusted and serviced by a qualified service technician only CAUTION The Tissue Tek AutoTEC is a precision instrument and must be handled accordingly Rough handling will disturb or damage internal components Always handle the instrument with care WARNING DO NOT PLUG IN THE AUTOTEC POWER CORD AN AUTHORIZED SERVICE TECHNICIAN MUST FIRST VERIFY THE POWER LINE MEETS SPECIFICATIONS Environmental Factors The Tissue Tek Auto
20. 800 725 8723 Outside the United States contact the nearest authorized Sakura Finetek instrument distributor e issue Tek Accu Edge Grossing Fork Cleaning Brushes product code 4814 10 pkg e issue Tek Accu Edge Grossing Board Tamper Set product code 4846 e Tissue Tek Accu Edge Grossing Board Gauge Set product code 4847 e issue Tek Accu Edge Trimming Handle product code 4786 e Tissue Tek Accu Edge Trimming Blades product code 4785 50 pkg Tissue Tek Accu Edge Scalpel Handle product code 4791 e Tissue Tek Accu Edge Scalpel Blades 62 Pointed product code 4793 20 pkg Revised 5 19 2010 7 3 SERVICE AND REPLACEMENT PARTS 7 4 Revised 5 19 2010 Appendix GLOSSARY AutoTEC Base Molds Cassettes Cool Down Embedding Embedding Medium Frame Gripper Grossing Input Magazines Magazines Magazine Warm up Time Main Door Output Doors Paraffin Paraffin Trough Paraffin Reservoir Recovery Chute Sectionable Revised 5 19 2010 A reliable high quality automated precision instrument that embeds diagnostic tissue specimens continuously forming sectionable paraffin blocks Molds contained in the base mold assembly that are used to hold heat embed and cool standard biopsy or orientation specimens in cassettes Paraform components that are sectionable and hold tissue specimens during the embedding process These cassettes are available in s
21. M s DATE AND TIME Tuesday March 09 2010 5 29 18 PM A Es INSTITUTION Sakura R amp D LaB EXIT TOTAL CASSETTES WARM UP 3 10 2010 6 00 00 2 CASSETTES TO THE CHUTE COOL DOWN 3 10 2010 8 00 00 PM RESERVOIR TEMPERATURE 64 56 C PARAFFIN LEVEL GOOD Figure 3 T Operator view of the System Setup screen Revised 5 19 2010 Section 3 To set system parameters The RESTORE DEFAULTS restores all settings to their defaults Set up parameters defaults and functions are as follows Parameter Default Function Alarm Volume High Sets the volume level for alarms Selects alarm sound from Alarm 4 seven options Sets sound for end process Alarm Sound End Process End Process 5 sound indication from seven options Date and Time M the date and time to be The following pages review in details each of the user defined parameters Languages Setup As shown in Figure 3 U in addition to English the following 15 language choices can be selected French Dutch Spanish Italian German Swedish Finnish Danish Greek Norwegian Czech Russian Japanese Korean and Portuguese Mar 10 2010 Sakura RAD LaB 92 AutoTEC WEDNESDAY 09 13 35 2 m E OG ALARM VOLUME TEST ALARM TEST END RESTORE DEFAULTS ALARM SOUND END PROCESS SOUND Dan DATE AND TIME INSTITUTION Sakura R amp D LaB SAVE eu EXIT
22. MAGAZINE When displayed it allows the user to request access to the input door Once the instrument has initialized the door mechanism the ID interlock is released and magazines can be removed and inserted Inserted magazines will be subject to a 15 minute warm up period before embedding starts NOTE If previously inserted magazines have already been warmed for 15 minutes embedding will automatically start or resume for these magazines as soon as the door is closed and locked At the bottom of every screen Total number of cassettes embedded the number of cassettes thrown to the chute and the reservoir paraffin temperature are displayed at the lower left corner of the screen next programmed warm up time cool down time and the current reservoir paraffin fill level status are displayed at the lower right corner of the screen indicated Paraffin levels are e Good e Low the xxx number inside the parenthesis is the numbers of cassettes that can be embedded with the amount of paraffin left inside the reservoir When paraffin level is first detected Low 128 cassettes can still be embedded without any refill This number counts down from 128 to 0 HELP When selected pops up context sensitive help menu Revised 5 19 2010 UTILITIES Screen Utility screen is displayed when the UTILITIES tab is selected Figure 3 D Feb 02 2010 Sakura R amp D Lab D 22
23. ON and LOG OFF The instrument 5 then ready to receive cassettes and embed them Touching LOAD MAGAZINES button will give access to the input door An on screen message will confirm that the input door is ready to be opened Revised 5 19 2010 Load Magazines Usually the Paraform Cassettes are assembled and inserted into the magazines at the grossing station Magazines standard or Tissue Tek Xpress Rapid Tissue Processor type Standard magazines can hold up to 32 Paraform cassettes These magazines are compatible with the Tissue Tek VIP tissue processors Xpress magazines can hold up to 20 Paraform Cassettes Figure 4 E shows an example of each type of magazine Figure 4 E Xpress Magazine with Adapter and Lid Standard Magazine with Lid removed Figure 4 F Cassette Retainer in position Revised 5 19 2010 Section 4 To load magazines into the input door follow these instructions 1 Properly assembled and placed frame cassettes inside the magazine are necessary for uninterrupted embedding operation Please verify a Check the orientation of cassettes in the magazines The top sides should be facing up and the angled writing surfaces of the frames should be toward the magazine cover b Ensure that all frames are parallel none fallen or tipped backward c When holding a magazine filled with frames and viewing from the sides there should be no gap between the frames gap fo
24. Tissue Tek 0 220 ERRORLOG RUNHISTORY READY SHUTDOWN SOFTWARE VERSION m Cool Down Mode USER ACCOUNTS SYSTEM SETUP SHUT DOWN Dai CLEAN GRIPPER ARMS TOTAL CASSETTES 0 WARM UP 2 3 2010 8 00 00 2 CASSETTES TO THE CHUTE COOL DOWN 2 2 2010 10 00 00 PM 64 08 C PARAFFIN LEVEL GOOD RESERVOIR TEMPERATURE Figure 3 D Button Descriptions A USER ACCOUNTS When selected is used to manage user accounts such as Add Edit and Delete users READY COOL DOWN TIMES When selected is used to set up automatic system ready and cool down times for each day of the week or to control this ready cool down time manually This is also used to select off days SOFTWARE VERSION When selected is used to display the current AutoTEC software version Revised 5 19 2010 Section 3 a SYSTEM SETUP When selected is used to set up alarm volume alarm sound end of process sound current year date and time and institution name SHUT DOWN When selected is used to shut down the AutoTEC Software applications Windows operating system PC and instrument COOL DOWN Mode mamm WARM UP Mode When selected will either shut down all AutoTEC heaters or start warming up all AutoTEC heaters When in Cool Down mode the AutoTEC Software application is still operational but the system cannot embed cassettes until all the heaters are up
25. day off Figure 3 M 1 Select the DAYS OFF tab 2 Use the arrow buttons located in the upper left and upper right corners of the calendar to change the month 3 Touch on the date on the calendar to set as off The chosen date will be circled in red on the calendar as will any other days off in the displayed month To delete a date and restore it to a working day e Either touch the date on the calendar the red circle will disappear When all days off have been scheduled touch the SAVE AND EXIT button Sakura RED LaB B Sakura RUN HISTORY A Jan28 2010 Tissue Tek AutoTEC 20 44 ERROR LOG SAVE AND EXIT EXIT TOTAL CASSETTES 0 WARM UP 1 28 2010 7 00 00 amp M 2 CASSETTES THE CHUTE 0 1 28 2010 10 00 00 PM RESERVOIR TEMPERATURE 27 78 C PARAFFIN LEVEL GOOD Figure 3 M Days Off screen Revised 5 19 2010 Shut Down Touch the Shut Down icon from the Utilities Selection screen to initiate system shut down When the Shut Down icon is touched a prompt Figure 3 N appears requesting confirmation that the system should shut down Do you really want to shut down the AutoTEC No Yes Figure 3 N Shutdown Confirmation Prompt NOTE When shut down is confirmed the AutoTEC program Window operating system and PC will completely turn off To restart the Auto TEC Software the operator needs to cycle the AC circuit breaker
26. generate the message shown in Figure 6 If the problem persists remove the magazine and reseat the cassettes If this action does not correct the problem try a different magazine If the problem Revised 5 19 2010 Standard Close the front door and press CONTINUE CONTINUE Figure 6 J Cassette Lid Not Closed If the instrument detects that a Paraform Cassette lid is open the gripper will place the cassette on a standard base mold without performing the Cassette Type check and generate the message shown in Figure 6 J Follow the instructions the message screen and press CONTINUE then the gripper will retrieve a new cassette from the input door and continue 6 9 TROUBLESHOOTING 6 10 Revised 5 19 2010 Section 7 SERVICE AND REPLACEMENT PARTS Service If problem occurs with the Tissue Tek9 AutoTEC that cannot be solved by implementing the recommended corrective actions listed in Section 6 Troubleshooting and instrument failure is apparent the Sakura Finetek Technical Support Department is available to assist Prior to contacting the Technical Support Department obtain model number installation date and warranty period information which will be required by the Technical Support Representative Record this information in the blanks below for quick reference AutoTEC Model No AutoTEC Serial No Installation Date Warranty Period NOTE To determine the model number of the AutoTEC instrument che
27. of frames inside the magazines If after all retries the gripper still cannot grab a frame from the magazine the system will alert the operator and wait for the operator to resolve the cassette problem If the operator does not respond in 30 seconds the AutoTEC will skip the trouble magazine and start to process the next available magazine INTRODUCTION Figure 1 F Cassette Sensors Paraform Recovery Chute Chute Count Sensor and Wide Beam Sensor Once a Paraform Cassette has been picked and validated by the sensors a small heating unit is engaged to keep the cassette warm during transportation to the base mold The gripper will then move the cassette to the base mold module Figure 1 6 Figure 1 G The Gripper places a 13x13 Paraform Cassette in a Base mold The Teflon coated base mold module comprises three channels of eight base molds each The base molds in the first channel closest to the back of the system are sized to accept the larger standard Paraform Cassettes The base molds in the second channel accept the 26x19mm biopsy and other specialty Paraform Cassettes The third channel accepts the 13x13mm Biopsy Cassettes Each 1 4 individual base mold is a Thermo Electric Cooler TEC acting as a heating and cooling element The stager and its paraffin dispensing mechanism performs the embedding of the Paraform Cassettes as follows First the stager assembly positions the paraffin dispensing mechanism heated
28. of the instrument through its front or rear doors without making an on screen request to do so The doors are interlocked and opening them without request will require a system re boot When it is necessary to access the interior of the system through its front or rear doors the system will show an on screen prompt like the one in Figure 6 A The system will stop embedding when a door is opened power turns off disabling the robot and stopping the base mold fans Open the front door and if necessary the rear doors and retrieve the missing block from base mold 1 Remove any excess paraffin or obstruction around the base mold Once the block has been retrieved close and latch all doors then touch OK to resume instrument operation OK Figure 6 A Prompt Requiring Instrument Access Touching INSTRUMENT ACCESS will have the same effect except that a pop up dialog will appear Figure 6 B Doors may now be opened To resume operation close and latch all doors then touch OK OK Figure 6 B Instrument Access Request Acknowledged 6 7 TROUBLESHOOTING For rear access use a 5 32 Amm Allen wrench to turn the two latches and swing the doors open This tool is included in the AutoTEC accessory kit To access the base molds from the front use the Allen wrench to release the two door latches Figure 6 C Figure 6 D Front Door Open Once access is complete and the doors are closed and latch
29. please perform gripper maintenance cleaning at this time Touch ABORT to do so OR touch RESUME to continue with current operation and defer this to another time Instrument will automatically RESUME current operation in 1 77 seconds RESUME ABORT Figure 5 K To clean the gripper arms select ABORT on the above message box If the time is exceeded the system will resume current operation in 180 seconds To override cleaning at this time select RESUME to let the instrument continue with the current task If operator selects ABORT to clean the gripper arms then the stager will move over standard base mold 1 and gripper will move above base mold 5 giving enough clearance to clean the bottom of the gripper arms Once in the position wait for the following message to be displayed on the screen before attempting to open the door Figure 5 1 Revised 5 19 2010 Section 5 Please open the front door and carefully wipe off any paraffin drops or accumulation from the bottom of the gripper arms When done please close front door and touch OK to continue Figure 5 L Open the front door to clean the gripper arms Figure 5 and follow Gripper Arms Cleaning Procedure Figure 5 M 9 9 Gripper Arms Cleaning Procedure The instrument will prompt the operator to clean the gripper arms once a week Figure 5 N 2 Gripper Arms Figure 5 N
30. the means of viewing the current system settings of selecting and configuring system options and specifying the desired values for user configurable operating parameters This section provides the procedures to perform initial setup Perform the following setup procedures before routinely using the Tissue Tek AutoTEC system In addition perform these procedures as needed to reset the clock add or remove users change automatic start up and cool down times etc Access Levels user can have one of two access levels e Administrator Users with administrative status can navigate through all available screens to perform functions including system setup run history tracking and start up and cool down programming e Operator Users with operator status can only navigate the main screens general operational functions and some utilities screens See page 3 7 for details Up to 55 names and passwords can be active at any time 13 Administrators and 42 Operators The type of user currently logged in is identified by an icon that appears in the top right corner of each screen Administrator Operator Page 3 14 User Accounts describes how to set up names and passwords 3 6 Accessing the UTILITIES Menu NOTE Login as an Administrator in order to set up or modify system settings through the UTILITIES menu Select the LOG IN button to access the Enter Password keypad A valid password must be entered to L
31. the robotic system is interacting with the doors Each of the four output doors includes a spring loaded matrix that holds embedded blocks firmly in place yet enables them to be removed easily Each door accepts up to 32 Paraform blocks When the touch screen monitor displays that access is granted the door can be removed by pressing the associated red interlock button The removed output door can be used as a secure tray to transport embedded cassettes to the next phase of the histological process Extra output doors are provided so that the continuous flow is not interrupted waiting for the output door to be returned Revised 5 19 2010 Section 1 Paraffin Reservoir Figure 1 J Figure 1 J Paraffin Reservoir Lid Open The paraffin reservoir stores and heats up to 5 38 liters of paraffin This is enough to embed up to 960 Paraform cassettes or approximately eight hours of operation For superior results and to minimize the production of bubbles only use the Tissue Tek Paraform Processing Embedding Medium Formula 3 product code 7052 The paraffin reservoir includes an ultrasonic sensor to detect the paraffin level in the reservoir and a filter assembly that prevents foreign particles from entering the paraffin delivery tube The filter can be accessed by removing the black cap clip on paraffin fill gauge is located at the corner of the reservoir to indicate the number of paraffin bags that can be added to fill t
32. to return to the System Setup screen Feb 23 2010 Sakura Dream Lab Ay 32 Tissue Tek AutoTEC lt ERROR LOG RUNHISTORY SERVICE TIMES READY COOLDOWN TIME SUNDAY NOT USED NOT USED MONDAY 06 00 AM 11 00 TUESDAY 06 00 AM 07 00 WEDNESDAY 06 00 AM 12 06 Lo e SAVE AND EXIT THURSDAY 06 00 AM 07 00 EU m er EXIT FRIDAY 06 00 AM 07 00 SATURDAY 06 00 AM 01 00 EVERY DAY AM PM TOTAL CASSETTES 0 WARM UP 2 24 2010 6 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 23 2010 7 00 00 RESERVOIR TEMPERATURE 54 88 C PARAFFIN LEVEL GOOD Figure 3 L Ready Cool Down Times screen 3 8 NOTE The times set in this screen will repeat weekly unless Days Off is specified NOTE Auto At least one ON TIME and one OFF TIME must be set NOTE To control the Ready Cool Down time manually select the MANUAL radial button NO TIME SET appears for the WARM UP and COOL DOWN time in the MONITOR screen Days Off Selection Use the Days Off screen to override the automatic timers on specific days that the AutoTEC will not be operating due to holidays vacations or other scheduled days off On days selected here the AutoTEC will not automatically start its warm up procedure To schedule a
33. to operating temperature 7 CLEAN GRIPPER ARMS When selected is used to request the gripper arms to be moved to above base mold 5 position to allow operator easy access to clean the gripper arms 3 3 CUSTOMIZATION OF SETTINGS ERROR LOG Screen ERROR LOG screen is displayed when the ERROR LOG tab is selected Figure 3 E a 11 2010 Sakura R amp D Lab ze Tissue Tek AutoTEC rere b ALL User Save EnlD ShortDescription Date amp TimeOccurred User MessageTitle 122622010 11 30 Sakura SENSORS MALFUNCTIONING 59 The followi Base Mold Temperature Problem 1 26 2010 11 29 Base Mold Temperature Problem 23 Sensors Malfunctioning 1 26 2010 10 16 AM Builtln VERSAGENDE SENSOREN 59 i Base Mold Temperature Problem 1 26 2010 10 16 Builtln Base Mold Temperature Problem 23 Base Mold Temperature Problem 1 26 2010 10 16 AM Base Mold Temperature Problem 23 Base Mold Temperature Problem 1 728 201 010 15 Base Mold Temperature Problem 23 Axis Stall In The Y Axis 1 26 2010 10 06 Service AXIS STALL 11 Stall In The Y Axis 1 26 2010 10 00 Service AXIS STALL 11 Stall In The Y Axis 1 26 2010 9 57 Service AXIS STALL 11 TOTAL CASSETTES 8 WARM UP 2 11 2010 8 00 00
34. 2 Operators can be set up Feb 11 2010 Sakura R amp D Lab 8 9 AutoTEC jocos orsessay Dn weap ane RY TOTAL CASSETTES WARM UP 2 11 2010 8 00 00 2 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 5 00 00 PM RESERVOIR TEMPERATURE 47 25 C PARAFFIN LEVEL GOOD Figure 3 BB Utilities screen Select ADD to add a new user Use the keyboard display to type in the information for the new user Figure 3 CC Highlight a user from the UserName list then select the EDIT to edit an existing user Highlight a user from the UserName list then select the DELETE to delete an existing user 2 e Feb11 2010 Sakura R amp D Lab SE Tissue Tek Dos s PERSE REESE ED ER EE RC RADI SIS It Verify Password HEBGBUBOUOUOUS oem i ema TOTAL CASSETTES WARM UP 2 11 2010 8 00 00 AM CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 5 00 00 PM RESERVOIR TEMPERATURE 47 35 C PARAFFIN LEVEL GOOD Figure 3 CC Add a new user 3 14 NOTE Passwords and user names can only have a maximum of 8 characters NOTE Administrators may change or delete passwords and user names but passwords are never displayed If an operator forgets their password it can be reset Touch the User Name Password Verify Password fields and use the display keyboard to type a na
35. 8 Revised 5 19 2010 TROUBLESHOOTING Error Recovery Selected components and system parameters are monitored when the Tissue Tek AutoTEC is turned on and while it is operating to ensure that the instrument is functioning properly If a monitored component or parameter falls outside established limits of operating criteria not only does an error message display in a pop up window on the control panel but also an audible alarm sounds Revised 5 19 2010 Section 6 Error messages are shown in the table below They include e Error codes e itles descriptions of errors Messages with instructions on the steps to take for error recovery e Actions required to recover from the error Document error codes for reference when contacting Sakura Finetek for service NOTE Internal events are recorded in the Error Log but are not considered errors which require corrective action An example of such an event is the second or third attempt of the gripper to pick up a framed cassette 6 1 TROUBLESHOOTING ERROR CODES AND MESSAGES Error Codes and Messages Error Title Result or Code Error Type Action Required XPECTED FRAME PROBLEM Unexpected Frame Problem User can interrupt the stall recovery process by touching ABORT button this allows user to access the instrument and move any axis manually if required User can touch RESUME button to immediately start stall recovery process This is a timed message box which
36. AM Base Mold Temperature Problem 23 Axis Stall In The Y Axis 1 26 2010 10 06 amp M 5 AXIS STALL 11 Axis Stall In The Y Axis 1 26 2010 10 00 AM Service AXIS STALL 11 pase Axis Stall In The Y Axis 1 26 2010 9 57 Service AXIS STALL 11 Base Mold Temperature Problem 23 TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 CASSETTES THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 RESERVOIR TEMPERATURE 73 21 C PARAFFIN LEVEL GOOD Figure 3 EE Error Log screen Error log can be sorted in the following ways to help viewing e By Date Ranges Select the pull down arrow for the From and To date to activate the date selection calendar Figure 3 FF Feb 11 2010 Sakura R amp D Lab 1 aes Tissue Tek AutoTEC 0 5 1 rere Sun Mon Tue Wed Thu 4T 2 4 5 7 8 9 10 HH 12 14 15 16 17 18 19 21 22 23 24 25 26 28 1 2 3 4 5 7 8 9 10 11 12 2 11 2010 TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 AM CASSETTES THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 RESERVOIR TEMPERATURE 4921 C PARAFFIN LEVEL GOOD Figure 3 FF Revised 5 19 2010 Section 3 Error ID Select the Error ID pull down arrow to display the pull down menu Scroll to the selection Figure 3 GG m 11 2010 Sakura R amp D Lab A A 3 TissueTek AutoTEC soirs bos I AL types or eror pre
37. Cleaning the gripper arms should be performed during the cool down period Use dry foam swabs Product code 8050 or dry lint free cloths such as Kimwipes to remove the accumulated paraffin on gripper arms as shown in Figures 5 O amp 5 P CAUTION Do not use cotton Q tips or cotton cloth to clean the gripper arms Lint left from cleaning may interfere with the sensors 9 6 Figure 5 P After cleaning the gripper close the front door to allow the instrument to resume operation After closing the front door acknowledge message box on screen to continue with the cycle Figure 5 L NOTE Do not load magazines when 4 hours warm up timer is on screen Before loading the magazines please make sure the timer is NOT on screen either override the timer or wait 4 hours for the timer to go off The paraffin inside the delivery tubes may still be solidified and the instrument will not be ready for robotic system movement Revised 5 19 2010 2 Gripper Arms Parallel Check fails If Gripper Parallel Check fails the instrument prompts the operator to clean the gripper arms Figure 5 Q WARNING Gripper arms may have excessive paraffin accumulation To maintain proper operation of the AutoTEC instrument please perform gripper maintenance cleaning at this time Touch ABORT to do so OR touch RESUME to continue with current operation and defer this to another time Instrument will automatically RESUME current operation in 174
38. D MISSING BLOCK PROBLEM U V STEPPER BOARD PROBLEM W X STEPPER BOARD PROBLEM Y Z STEPPER BOARD PROBLEM SOLENOID DRIVER BOARD PROBLEM HEATER CONTROLLER BOARD PROBLEM TEC BOARD PROBLEM PARAFORM CASSETTE INCORRECTLY POSITIONED IN BASE MOLD MISSING BLOCK FROM BASE MOLD U V STEPPER BOARD NOT RESPONDING W X STEPPER BOARD NOT RESPONDING Y Z STEPPER BOARD NOT RESPONDING SOLENOID DRIVER BOARD NOT RESPONDING HEATER CONTROLLER BOARD NOT RESPONDING TEC BOARD NOT RESPONDING Open the front door and reset the Paraform Cassette in base mold lt 1 8 gt cassette type Once the cassette has been reset close and latch the front door then touch OK to resume instrument operation Open the front door and if necessary the rear doors and retrieve the missing block from base mold lt 1 8 gt Remove any excess paraffin or obstruction around the base mold Once the block has been retrieved close and latch all doors then touch OK to resume instrument operation U V Stepper Board is not responding System is trying to recover the error to resume current operation W X Stepper Board is not responding System is trying to recover the error to resume current operation Y Z Stepper Board is not responding System is trying to recover the error to resume current operation The Solenoid Driver Board is not responding Please contact Sakura Service department for further instructions Please rem
39. E CHUTE 0 1 26 2010 10 00 00 PM RESERVOIR TEMPERATURE 63 57 C PARAFFIN LEVEL GOOD Figure 3 R Software Version screen amp Clean Gripper Arms Touch the Clean Gripper Arms icon to request the gripper arms to be moved to the area above the base mold 5 position allowing operators easy access to clean the gripper arms Revised 5 19 2010 System Setup The System Setup screen Figure 3 S enables the administrator to set a variety of system parameters Operators can only view the operator view of the System Setup screen Figure 3 T Mar 10 2010 Sakura R amp D LaB 2 i SETissueTek AutoTEC weonespavo1z _ ia LANGUAGE English ALARM VOLUME LOW TEST ALARM TEST END RESTORE DEFAULTS ALARM SOUND ALARM2 END PROCESS SOUND End Of Processi DATE AND TIME Tuesday March 09 2010 x 5 29 18 PM 4 2 INSTITUTION Sakura R amp D LaB J TOTAL CASSETTES WARM UP 3 10 2010 6 00 00 2 CASSETTES TO THE CHUTE 0 COOL DOWN 3 10 2010 8 00 00 PM RESERVOIR TEMPERATURE 63 98 C PARAFFIN LEVEL GOOD Figure 3 S Administrator view System Setup screen Mar 10 2010 Sakura RAD LaB B 92 Tissue Tek AutoTEC WEDNESDAY 09 11 19 AM Ems RUN HISTORY LANGUAGE English Y ALARM VOLUME row 71 TEST ALARM ALARM SOUND ALARMI 71 TEST END END PROCESS SOUND End Of Process 1
40. Instrument componens the crebri 1 2 M Magazines NIRE E 4 5 enue EN DL 4 6 eres T 4 3 4 3 EE 4 3 Main terii cue t P 2 2 Maintenance 5 I ea 5 1 Gripper Arm 5 6 Magazine 04400 00 5 4 Output grid sensor cover glass cleaning 5 2 Paraffin Trough Cleaning Replacement 5 1 RESEO ncm TEM 9 2 Reservoir filter 9 2 Missing or Damaged ltems sess 2 1 0 On screen color codes BaSe 4 5 Embedding tracking 4 6 MAG AZ 4 5 QUID GOOFS 4 5 Operation AAA paraffin 4 7 End 809 Da a 4 6 Load Magazines cedes 4 3 pe 4 1 Operating sequence 4 1 liz llt zn fe qo co 4 2 SIMUL COW M 3 3 e 3 3 4 2 Operator Ine Td D b bn 1 6 B 1 INDEX P Parafin bd beu 4 7 Par
41. OFF ON Shut down is equivalent to a hard reset and restart of the AutoTEC hardware and software NOTE Before the AutoTEC actually starts the shut down process a message will be prompted to the operator to remove all magazines inside the Input Doors Cool Down Mode Touch the Cool Down mode icon from the Utilities Selection screen to initiate system cool down When the Cool Down mode icon is touched a prompt Figure 3 O appears requesting confirmation that the system should cool down and allow the paraffin to cool NOTE In the Cool Down mode the system cannot embed cassettes A warm up time of up to 4 hours will be needed before embedding can start Revised 5 19 2010 Section 3 NOTE Ita Cool Down time was programmed for that day this forced cool down will override that time for the current day only The programmed time will remain active for future days Refer to page 3 8 about how to program cool down times Are you sure you want to switch to Cool down mode No Yes Figure 3 O Cool Down Confirmation Prompt Once this prompt is acknowledged another Figure 3 P appears requesting that all magazines be removed from the input door Remove all magazines and touch OK OK Figure 3 P Magazine Removal Prompt The AutoTEC will not cool down until the input door is cleared of magazines and closed and the on screen prompt is acknowledged The Utilities screen continues to display during this p
42. ONDAY 01 50 00 PM ADMIN ERRORLOG RUN HISTORY TOTAL CASSETTES WARM UP 2 15 2010 8 00 00 2 CASSETTES THE CHUTE 0 COOL DOWN 2 15 2010 10 00 00 PM 1 RESERVOIR TEMPERATURE 63 76 C PARAFFIN LEVEL GOOD Figure 4 L Run screen Magazines Heated NOTE The input door sensor system is able to detect the presence of magazines and if they contain cassettes or are empty However it cannot tell in advance how many cassettes are present in a given magazine when first loaded The system counts cassettes as they are removed from each magazine and displays that total below the input magazine graphic End of Run and Removing Output Doors By definition a run consists of all cassettes in a magazine Once the cassettes initially loaded into a magazine are all embedded and transferred to an output door an audible alarm sounds and a prompt will appear in the message area at the bottom of the screen indicating that the run is complete for that magazine Within 1 2 seconds the completed output door will be highlighted When the output doors have been removed the display will update the status of the output door within 1 3 seconds If no cassettes are being embedded and as the last door is removed it then takes 30 seconds to update the door status When the door is removed the count beneath is also reset simultaneously and the message at the bottom of the screen now only reflects the current status If all output doors are removed
43. T or RESUME Touch OK 6 5 CLEAN SPLASH GUARD Maximum number of cassettes to embed exceeded GRIPPER amp ID PANEL 1000 must clean splash guard gripper BM covers ID back openings TROUBLESHOOTING RUN ABORTED DUE TO Run aborted due to unparallel gripper arms bending RUN ABORTED UNPARALLEL GRIPPER upwards failed to block sensor bending downwards Touch OK ARMS Could not Power On the instrument Clear all base molds POWER ON COULD NOT POWER ON and empty output doors while this message is displayed Contact Sakura PROBLEM THE INSTRUMENT PLEASE DO NOT TURN OFF THE MACHINE Contact Service Department Sakura Service Department for further instructions AMBIENT TEMPERATURE Ambient Temperature exceeds operating requirements EXCEEDS ACCEPTABLE Embedding Stopped until ambient temperature lowered OPERATING TEMPERATURE AMBIENT TEMPERATURE PROBLEM 6 6 Revised 5 19 2010 Error Code Notes For errors 13 through 22 the error number will vary depending on the subsystem affected lt 1 gt denotes a range of numbers that may appear in a message referring to Input Door chambers Base Molds or Output Doors depending on the operating status Error Recovery Procedure If an error message that could lead to system shut down displays the Sakura Finetek Service department will instruct the user to take the following steps to attempt to recover the error 1 Remove all paraffin blocks from each of the outpu
44. TAL CASSETTES 0 WARM UP 2 16 2010 8 00 00 AM CASSETTES TO THE CHUTE COOL DOWN 2 15 2010 10 00 00 PM RESERVOIR TEMPERATURE 63 58 C PARAFFIN LEVEL GOOD Figure 3 MM Summary page By selecting a date from the summary screen details of all the magazines ran on that day are displayed Figure 3 NN Revised 5 19 2010 Section 3 3 z 15 2010 SakuraFINETEK Je Tissue Tek AutoTEC MONDAY 11 09 28 AM ADMIN _ From To 5 SERIE E Summary Daily Daily Total Total Total Total Total MPH Load 3eady Start End Abort Pulled ETS amp 2 7 2010 Seen oe assette STD BX 3pecia 13x13 Others Time Time Time Time Time Time Log User 2 15 118 Fe pepe pe p pe 3 mes ps o De s n n n sn 12 Ties Els Magazine3 1 2 3 o da pee Ser Magazine4 17 2 E 12 PVE 112 Ser Magazine5 1 4 L Magazine6li 2 A 2 10 2010 2712 2010 TOTAL CASSETTES 0 WARM UP 2 16 2010 8 00 00 AM CASSETTES TO THE CHUTE COOL DOWN 2 15 2010 10 00 00 PM RESERVOIR TEMPERATURE 64 09 C PARAFFIN LEVEL GOOD Figure 3 NN One day view By selecting a magazine from the summary screen details of all the cassettes in this magazine are displayed Figure 3 OO Febi5 2010 SakuraFINETEK 3 5 32 Ss
45. TEC should be located in a temperate and humidity stable environment avoiding prolonged exposure to excessive temperature and humidity Temperature and humidity should be held relatively constant to obtain the highest degree of operating stability Nominal ambient X operating temperature conditions are 22 2 71 6 F 3 6 Nominal operating humidity conditions are 40 20 relative humidity Locate the instrument in a well ventilated area avoiding exposure to corrosive vapors direct air currents or temperature extremes Avoid exposure to direct sunlight open windows sinks ovens open flames hot plates radiators and dry ice baths Install the instrument on a solid level floor surface away from any equipment that consumes a high voltage or large current including large refrigerators and ovens For proper operation maintain a six inch 15 24 cm minimum clearance around the sides and back of the instrument For additional stability lower the feet located on the bottom of the instrument To lower the feet turn the nut fastened to the threaded shaft of each foot clockwise Revised 5 19 2010 Section 2 until the foot contacts the ground but without lifting the caster off the ground Unpacking and Installation The Tissue Tek AutoTEC is shipped in a large carton Inspect the carton for shipping damage If there is visible shipping damage immediately file a complaint with the carrier and notify Sakura Finetek or t
46. TER SERVICE SETTINGS AKIS MOTORS TEMPERATURES amp LOGS BASE MOLDS SOLENOID SENSOR BOARDS TOUCH SCREEN CALIBRATION TOTAL CASSETTES 0 WARM UP 1 27 2010 7 00 00 AM gt CASSETTES TO THE CHUTE 0 COOL DOWN 1 26 2010 10 00 00 PM m RESERVOIR TEMPERATURE 64 3 C PARAFFIN LEVEL GOOD Figure 3 G Please refer to the Service Manual for more details Revised 5 19 2010 Map of Interface Screens Figure 3 H illustrates the general structure of the interface available for an administrator level clearance showing screens accessible from the Monitor screen Wonitor screens with various buttons messages System Start Up Monitor Screen Initial UTILITIES LOGIN ERROR LOG RUN HISTORY Revised 5 19 2010 1 gt o USER ACCOUNTS FEAT js DELETE READY COOLDOWN COOLDOWN ON TIME TIMES DAYS OFF LANGUAGE ALARM VOLUME ALARM SOUND SHUT DOWN END PROCESS SOUND DATE amp TIME CLEAN GRIPPER FORMAT INSTITUTION COOL DOWN WARM UP MODE MODE SORT BY ERROR ID SORT BY USER TOTAL NUMBER OF ERRORS SAVE FROM TO DATE o SOFTWARE VERSION SYSTEM SETUP Figure 3 H Section 3 OFF TIME 3 5 CUSTOMIZATION OF SETTINGS The Tissue Tek AutoTEC allows for the customization of a number of system options and operating parameters to suit the requirements of a given laboratory The UTILITIES screen provides
47. TES 0 WARM UP 2 11 2010 8 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 PM RESERVOIR TEMPERATURE 321 C PARAFFIN LEVEL GOOD Figure 3 JJ 3 16 Output files can be saved in any one of the three formats HTML PDF or EXCEL to help in organizing the collected data NOTE Internal events are recorded in the Error Log but are not considered errors which require corrective action An example of such an event is the second or third attempt of the gripper to pick up a framed cassette Refer to Section 6 entitled Troubleshooting for error recovery information RUN HISTORY By selecting the RUN HISTORY tab the RUN HISTORY screen is displayed Figure 3 KK The RUN HISTORY displays chronological run information of the selected days 4 Feb 11 2010 Sakura R amp D Lab 2 2 i Tissue Tek AutoTEC E Eum umm 212010 een E File Output Colas Summary Dat Number Magazines Magazines Total Total Total Total Total Other B Of Cycles Loaded Processed Cassettes STD BX Special 13413 Types TOTAL CASSETTES WARM UP 2 11 2010 8 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 RESERVOIR TEMPERATURE 56 01 C PARAFFIN LEVEL GOOD Figure 3 KK Run History screen Run history display choices are following selecting Today button see today s run selecting This Week button see this we
48. a 3 product code 7052 should be used disposable paper trough which is placed directly under the input door to collect the melted paraffin which is present on the magazines and Paraform Cassettes from the tissue processor A reservoir located on the top right side panel of the AutoTEC that stores and heats up to 5 38 liters of paraffin sufficient to embed at least 960 samples Hinged door on left side panel of the AutoTEC permitting the retrieval of improperly gripped frames and cassettes Paraform Cassettes retrieval does not interrupt processing A characteristic of a cassette that enables paraffin blocked tissue to be sectioned using a microtome ready for placement on slides 1 GLOSSARY Stager Assembly Ready Mode System Warm Up Tissue Tek AutoTEC Tissue Tek Xpress Tissue Tek Paraform A 2 Positions the paraffin dispensing mechanism over the cassette in the base mold presses down on the cassette to ensure it is correctly positioned in its frame and dispenses an even flow of molten paraffin to the correct depth Condition in which the system is warmed up but is not embedding The AutoTEC program mode during the four hour period of time required to melt the paraffin in the paraffin reservoir and to bring the instrument to operating temperature See AutoTEC An instrument used to prepare tissue specimens for embedding The Xpress uses low power microwave energy to reduce processing time to one hou
49. aform recovery chute 1 4 Passwords 1 0 3 6 3 14 Programming Screens Audible alarm setup eese 3 12 Date and Time 3 13 Days OF Selection airan 3 8 Enter DassWOLUS Uude 3 14 Se UD octets rhet tu tQ ES 3 13 1 3 11 Ready Cool Down Times sess 3 8 RUN FIIS te 3 4 VSEM ttt tdt eta tne soda 3 11 555 52 saei 3 14 Recovery ETE 1 4 Regulatory information eese 1 7 Replacement parts how to is FRODOUG SY 51 5100 2 EN 1 3 FUIS ONY 3 4 3 16 RUN Scleeh EaVollbos a dei 4 5 S Safety Information sss 1 1 1 7 Screens DOWN anu tortue tetrum toot 3 8 3 9 Embedding in progress 2 222 0 4 6 3 4 4 2 Start
50. ation Operation WILL NOT continue until Output Door 4 is in 2 place empty Once the Output Door has been Touch OK replaced touch OK to resume operation Revised 5 19 2010 6 3 TROUBLESHOOTING OUTPUT DOOR 1 4 IS REQUEST NOT EMPTY 29 38 6 4 HEATER TEMPERATURE PROBLEM HEATER TEMPERATURE PROBLEM CASSETTE BLOCK PROBLEM FRAME HEATER OUT OF RANGE FRAME HEATER RTD IS OPEN DROPPED CASSETTE BLOCK INSIDE THE INSTRUMENT The Frame Heater temperature is out of acceptable range Min Max Please empty all input amp output doors and contact Sakura Service Department for further instructions Please touch RESUME to restart the application or ABORT to shutdown the instrument The GRIPPER FRAME HEATER RTD is Open The instrument MUST be serviced before embedding can continue Please empty Output Door lt 1 4 gt then touch OK to resume instrument operation A cassette block might have been dropped inside the instrument Please open front door and if required back doors and inspect for any dropped cassette block and retrieve it Close all the doors and please touch OK when done Perform action s requested Touch OK Touch OK Touch OK MISSING CASSETTE NO CASSETTE DETECTED IN FRAME ed MISSING CASSETTE NO FRAME DETECTED IN GRIPPER Chute message displayed FRAME POSITION PROBLEM MISSING BLOCK BLOCK NOT REMOVED PROBLEM FROM BASE MOL
51. ck the Serial Rating label at the lower left corner of the right side panel of the instrument or touch the Software Version icon in the Utilities screen Where to Call for Service If located within the United States contact the Technical Support Department of Sakura Finetek USA Inc by calling toll free 1 800 725 8723 Menu Option 2 In countries other than the United States contact the nearest authorized Sakura Finetek instrument distributor or representative for service information and technical assistance Replacement Parts Replacement parts include e User replacement accessories e Consumables Revised 5 19 2010 User Replaceable Accessories Accessories for the Tissue Tek9 AutoTEC include e 32 cassette magazine product code 7005 includes one cover Compatible with the Tissue Tek9 VIP9 tissue processors 4 pkg e 20 cassette product code 7006 includes one cover and 1 adapter Compatible with the Tissue Tek Xpress Rapid Tissue Processor 4 pkg e Handles for Tissue Tek Xpress compatible magazines product code 7007 6 pkg e Paraffin Scraper product code 1550 e Output Doors product code 7008 2 pkg e Paraffin Trough product code 7010 7 1 SERVICE AND REPLACEMENT PARTS Consumables e Tissue Tek Paraform Processing Embedding Medium Formula 3 product code 7052 8x1kg e issue Tek Paraffin Trough product code 7011 12 paper troughs cs e issue Tek9 Paraform
52. e triggered Please empty all input amp output doors and contact Sakura Service Department for further instructions Please touch OK to shut down the instrument BOTH THE LIMIT SWITCHES OF FRAME HEATER MOTOR ARE TRIGGERED Instrument shuts down if OK is touched The I O Board is not responding Please contact Sakura Service department for further instructions Instrument shuts down if OK is touched I O BOARD NOT RESPONDING Please remove all input magazines and empty all output doors then touch OK to shut down the system Base mold lt 1 8 gt has been disabled due to lt High Low Heating Cooling Temperature on Cassette Type TEC DISABLED Error Log Initiate the process to disable the particular base mold Abort the process by touching on ABORT or expedite the current process by touching on RESUME A timed message box appears for the user to act upon after 180 seconds and no response from the user it will disable the particular base mold and continue working with the next cassette and base mold Touch RESUME to disable or touch ABORT to continue current operation without disabling the base mold THERE HAVE BEEN THREE CONSECUTIVE V AXIS STALLS ON A PARTICULAR BASE MOLD 3 CONSECUTIVE ABORT The user selected three consecutive ABORT commands COMMANDS ON BASE mold lt 1 8 gt System will now disable base mold Touch OK MOLD SELECTED lt 1 8 gt BLOCK NOT REMOVED Base mold l
53. ed touching CONTINUE or OK in the dialog will return the system to normal operation If any robotic part was moved while the instrument was open the system will automatically go to its original configuration and then take up embedding where it left off Disabling Base Molds If the instrument was accessed but a block is still detected in the base mold the system will prompt the operator to remove the block again Figure 6 A After three unsuccessful attempts to remove the block the system will disable the base mold in question 6 8 NOTE Only users with a service level log in can re enable a base mold once it is disabled Disabling a base mold slows throughput since blocks must be cooled for the same amount of time regardless of how many base molds are in operation Disabled base molds should be replaced and serviced as soon as possible Disabled Base Molds A disabled base mold appears on screen without contents as seen in figure 6 4 FRG T E CT SATS Figure 6 E On Screen Display of Disabled Base Mold Disabling More Than Three Base Molds NOTE The occurrence of this situation is very unlikely The instrument will still run with four or up to seven base molds disabled if three base molds are disabled and the operator attempts to disable a fourth a message will appear Figure 6 F to warn the operator that the system throughput will slow down Do not attempt to load more magazines The n
54. efficiency consistency and standardization of laboratory procedures and operations e Provide robotic precision and consistency minimize the bottom paraffin layer and eliminate excess paraffin around the cassette border e Reduce labor requirements Revised 5 19 2010 Section 1 Tissue Tek Paraform Cassettes are made of two components an inner sectionable tissue insert and an outer frame that provides support when in the microtome chuck Paraform Cassettes are placed in up to four 32 or 20 cassette input magazines The magazines are loaded with the cassette front surface angled facing up during the embedding process Following a magazine warm up period of 15 minutes the instrument s robotic system e Sequentially removes each cassette from the magazines e identifies the type of cassette standard biopsy orientation core biopsy shaved biopsy or 13 x 13 e Places the cassette in the properly sized pre heated base mold e Pushes down stages the cassette until it reaches the bottom of the base mold e Dispenses a precise amount of paraffin to match the tissue size while holding the cassette in place The base mold cools to form a solid paraffin block The robotic system removes the paraffin block from the mold and presents it to sensors that verify that a frame 15 present and correctly positioned in the robotic gripper and that a paraffin block is in the frame The robotic system places the paraffin block in one of t
55. ek from Sunday to Saturday s runs selecting Last Week button see last week s Sunday to Saturday s runs selecting This Month button see this month s run specifying a date range using the From and To pull down arrow to activate the date selection calendar Revised 5 19 2010 NOTE When using date range after the date selections select the Go button first to update the display The Summary displays the days selected and the total numbers of cycles magazines cassettes types of cassettes etc Figures 3 LL and 3 MM Feb 11 2010 22 Tissue Tek AutoTEC THURSDAY 08 08 55 AM From 2 10 2010 2 11 2010 7 February 2010 Number Magazines Magazines Total Total Total Total Total Dther OfCycles Loaded Processed Cassettes STD BX Special 13 13 Types Sun Mon Tue Wed Thu Fri t 2 mu X 3 12 15 16 17 18 19 22 23 24 25 2 1 2 3 4 65 7 9 10 11 Today 2 11 2010 TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 AM CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 PM RESERVOIR TEMPERATURE 128 36 C PARAFFIN LEVEL GOOD Figure 3 LL Summary page 4 Febi5 2010 SakuraFINETEK 5 a E From 2 72010 7 Last Week This Month File Output El 8 Total Total us Total Total 2 7 2010 Loaded Processed Cassettes STD BX Special 13x13 EE 2 8 2010 2 9 2010 2n 0 2010 2 12 2010 TO
56. ent activities and displays a dialog box that enables the operator to either resume the activity or abort it To abort the person currently logged in must log in again The system would then transfer all cassettes present in the base molds to one of the output doors and will ask the operator to remove the magazines from the loading area Operation will then stop If the current login password is not entered or if no selection is made within 30 seconds operation will resume automatically NOTE The STOP button only appears while the system 5 embedding 4 LOG IN OR LOG OFF 1 LOG OFF toggle This is used to allow a user to Log in or Log off the system 1 LIGHT ON LIGHT OFF toggle This is used to turn the internal chamber light ON or OFF If left on the light will automatically turn off after 30 minutes of inactivity OVERRIDE OVERRIDE TIMER When displayed it allows the user to override the four hour warm up timer This button appears only if paraffin temperature has reached the factory set temperature cassettes are present and the magazines have been warmed for 15 minutes the system will start embedding when this button is pressed 2 ACCESS 2 INSTRUMENT ACCESS When displayed it allows the user to pause the system operation access the interior of the system by opening the front or rear doors and resume operation by touching OK in on screen prompt LOAD MAGAZINES LOAD
57. eople should move the Tissue Tek AutoTEC Although the instrument is on casters it is large and heavy and contains sensitive electronic components CAUTION When moving the Tissue Tek AutoTEC do not grasp or lift the instrument by its front panel or the side panels CAUTION Ensure that the feet are lowered to hold the instrument securely in place for stationary use CAUTION If AutoTEC is anchored to the building structure per California Building Code remove the securing hardware first before moving the AutoTEC Revised 5 19 2010 Section 3 CUSTOMIZATION OF SETTINGS General Information The four Tissue Tek AutoTEC main tabbed screens MONITOR UTILITIES ERROR LOG and RUN HISTORY are shown in the following figures Before a user logs in only the MONITOR screen is available for viewing the status of the instrument with all buttons disabled grayed out and tabs not functioning Figure 3 A 11 2010 Sakura R amp D Lab Tissue Tek AutoTEC jesse an j i YCC E TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 5 00 00 PM RESERVOIR TEMPERATURE 47 06 C PARAFFIN LEVEL GOOD Figure 3 A To log in to the AutoTEC press the LOG IN button on the MONITOR screen to access the Enter Password keypad Type in the password followed by pressing the ENTER button to log in Figure 3 B PLEASE ENTER PASSWORD KEE s e
58. h has 180 seconds RESUME to disable the base mold and continue with to respond to current operation message GRIPPER FAILURE BLOCK NOT REMOVED FROM BLOCK NOT REMOVED Touch ABORT to open the front door and remove the BASE MOLD FROM BASE MOLD block from base mold lt 1 8 gt and remove any excess 1 8 gt paraffin or obstructions on or near the base mold When Touch ABORT finished close and latch the front door and touch CONTINUE to resume the instrument operation If it is not possible to remove the block from the base mold close and latch the front door and touch CONTINUE The system will check the base mold and provide further instructions AXIS STALL IN THE U AXIS User can interrupt the stall recovery process by touching ABORT button this allows user to access the instrument and Touch CONTINUE ES AXIS STALL IN THE W AXIS move any axis manually if required User can touch RESUME button to immediately start stall recovery process Touch ABORT AXIS STALL AXIS STALL IN THE X AXIS This is a timed message box which is displayed for 180 or RESUME seconds only after 180 seconds and no response from the AXIS STALL IN THE Y AXIS user it will automatically start stall recovery process and will then resume the current operation This message is displayed AXIS STALL IN THE Z AXIS for each axis stall telling the axis name X Y Z etc 6 2 Revised 5 19 2010 Section 6 Error Codes and Messages Error Title 20 Resu
59. hange to display Today instead Figure 3 Il 20 7 TUESDAY 1D E208 AM ES OLLI Mina sa a Mapsi ETTE EEEN o Beer Communication Pisin EEE I 22 Base Ms Seres Cane ETT ase Serio samo Sores Base Mele Series 1271212010504 HeH Bare anne Base Fani ETTE 503201050020 EM pee TECE ase TECE Sario Bae ja Mah TEC Serie SHIS 2UID OR 2099 TEC E SAA Jaap Totma 1 Horn g WARM UF SA 7 0 A CASSETTES TO THE CHUTE AHTO Seno re RESERVOIR TEMPERATURE LEVEL S000 Figure 3 11 When this Today button is pressed the error listing will instead display only the errors that have occurred on the current date if any Also both the From and To dates will show today s date and the Today button will toggle back to an ALL button By selecting the Save button the selected Error log can be transferred to a Flash drive Figure 3 JJ Feb 11 2010 Sakura R amp D Lab sHunsoavorssocay 11 Feb 2010 11 Feb 210 LogiD ShortDescription Date amp TimeOccurred User MessageTitle Export To HTML PDF EXCEL TOTAL CASSET
60. he reservoir full without overfilling EH This can be relocated to any corner of the reservoir for better viewing INTRODUCTION Control Panel Figure 1 K The Tissue Tek AutoTEC operator interface is a touch screen control panel that operates a Windows based application program Jen 29 2010 Sakura R amp D LaB ERROR LOG RUN HISTORY TOTAL CASSETTES WARM UP 1 30 2010 7 00 00 gt CASSETTES TO THE CHUTE 0 COOL DOWN 1 30 2010 10 00 00 PM a RESERVOIR TEMPERATURE 54 38 C PARAFFIN LEVEL GOOD Figure 1 K The AutoTEC touch screen operator interface The touch screen diagonal size is 26 7 cm 10 5 in Custom application software includes the following modules e Instrument set up e Startup and Self checking e Password and security handling Run history e Graphical depiction of magazine and output door activities e Statistics setup and recording Error logging error checking and recovery e Automated warm up and cool down timing e Service maintenance and troubleshooting interfaces 1 6 Specifications Models Covered by this Manual Model Name Description Tissue Tek AutoTEC Automated Tissue Embedding Console 115 VAC USA Tissue Tek AutoTEC Automated Tissue Embedding Console 100 VAC Asia Tissue Tek AutoTEC Automated Tissue Embedding Console 230 VAC Europe 7000 7001 7002 Power Ratings and Requirements Model Power Frequency 7000 115 VAC
61. he distributor Standard Accessories Kit The following additional items are shipped with the Tissue Tek AutoTEC e Two spare output doors in addition to four installed on the AutoTEC e Paraffin Scraper e Door opening tool e Operating Manual this document e Quick Reference Guide One case 8 x 1kg of Tissue Tek Paraform Processing Embedding Medium Formula 3 product code 7052 Shipped separately e One package of paper disposable paraffin troughs 12 cs product code 7010 e One of the following options Six 32 position magazine assemblies if a Tissue Tek9 VIP9 Tissue Processor is in use in the Laboratory OR Six 20 position magazine assemblies and six handles if a Tissue Tek x Series Rapid Tissue Processor 15 in use in the Laboratory Missing or Damaged Items Verify that each item listed on the packing list is included in the carton Ensure that all packaging materials have been removed and carefully checked for all parts If any items are visibly damaged or are missing call 1 800 725 8723 or 1 310 972 7800 U S Customers only If located outside the U S contact the nearest Sakura Finetek instrument distributor 2 1 INSTALLATION Moving If it is necessary to move the AutoTEC to a new location allow the paraffin to cool enough to solidify before any movement occurs Once the main power switch has been turned off open the reservoir access door to let heat out to speed the paraffin c
62. he four 32 position output doors Each output door can be detached from the instrument and used as a transport tray to a microtome workstation oprings hold the paraffin blocks securely in place while enabling them to be easily removed Location of Major Systems and Components The Tissue Tek AutoTEC instrument is comprised of the following major systems and components Figures 1 A through 1 C A detailed description is given in the following paragraphs INTRODUCTION Location Figure 1 A Front View and Left Panel Component Touch screen monitor E KI Main door for access to input door output doors and paraffin 11 drip tray Front door eguipped with safety interlocks Allowing operator easy access to base molds and air filter Paraform recovery chute door for retrieval of rejected cassettes Paraffin reservoir lid opens to allow addition of paraffin to the system Paraffin reservoir filter access EI ESE E 1 2 Rear doors eguipped with safety interlocks give access to robotic system External computer system connections for PS2external pointing device such as a mouse 52 keyboard to supplement on screen keypads RJ 45 Port USB 2 0 peripherals including USB key drives used to export log files and other data Main power switch Power cord Input Door for access to the magazine loading area Disposable Paraffin Trough Four removable output doors each holding up to 32 blocks
63. hree banks of base molds are depicted below the magazines and doors The totals shown on the right of the base molds indicate how many of each type of cassettes have been embedded so far Feb 15 2010 SakuraFINETEK 32 Tissue Tek AutoTEC onnav01 5000pW ERRORLOG RUN HISTORY EE INSTRUMENT LOAD TOTAL CASSETTES 40 WARM UP 2 16 2010 8 00 00 2 CASSETTES THE CHUTE 0 COOL DOWN 2 15 2010 10 00 00 PM RESERVOIR TEMPERATURE 63 76 C PARAFFIN LEVEL GOOD Figure 4 J Run screen layout Run Screen Color Coding The Run screen displays the status of the magazines base molds and output doors using the following color coding NOTE As a general rule for the input magazines and output doors a dark border indicates that the magazine can either be removed and or a new one can be inserted and that the output door can be removed and an empty one placed Revised 5 19 2010 Section 4 Input Magazines Color Code Information Red Magazine is in the15 minute warm up period Pink Magazine warm up period is complete System is ready to embed Magazine emptied The number of cassettes processed displays below the graphic Cassettes are being taken out of the magazine The number of cassettes displayed is incremented each time a cassette is taken from the magazine Dark blue bars with dark border Dark blue bars dark border Gray with light border no bars No magazine is detected Base Molds a
64. is displayed for 180 AXIS STALL seconds only after 180 seconds and no response from the user it will automatically start stall recovery process and will then resume the current operation This message is displayed for each axis stall telling the axis name X Y Z etc and error number there are different error numbers for different axis Message 2 AXIS STALL IN THE U AXIS Touch ABORT or RESUME AXIS STALL IN THE INPUT DOOR Cassette dropped to chute while grabbing cassette from input door Cassette dropped to chute while checking frame Cassette dropped to chute while checking type of cassette 5 BLOCK CASSETTE BLOCK CASSETTE DROPPED C T Chute message TO CHUTE TO CHUTE 2 displayed Block Cassette dropped to chute during Error Recovery Block dropped to chute while picking from base mold lt 1 8 gt The gripper failed to open at mold Open the front door and reset the Paraform Cassette into base mold lt 1 8 gt GRIPPER FAILED TO OPEN Check the gripper and base mold for excess paraffin and Touch AT BASE MOLD obstructions Once the Paraform has been seated in the base CONTINUE mold close and latch the door then touch OK to resume instrument operation Touch ABORT to open the front door and remove the Touch ABORT or block from base mold lt 1 8 gt and remove any excessive RESUME User paraffin or obstructions on or near the base mold Touc
65. itic filter straight out from the reservoir Rotate by holding the top of the filter until center Figure 5 F tabs are turned away from the locked down positions Figure 5 D Figure 5 F Figure 5 D 8 Usea bath of solvent to clean the wax filter 9 Drythe filter and reinstall it in the reservoir Figure 5 4 Liftthe filter for cleaning Figure 5 E Figure 5 G Figure 5 E 10 Place the panel cover back 5 Use a bath of solvent to clean wax and debris out of the filter 6 Dry the filler and reinstall it in the reservoir taking care to tighten the screws down on the tabs of the filter Revised 5 19 2010 9 9 Magazine Cleaning Magazines must be free of excess paraffin before they are loaded with cassettes A dishwasher using a heated cycle can be used or alternatively the cleaning cycle of an overnight processor such as the Tissue Tek VIP Output Door Inspections and Cleaning Output doors must be periodically inspected for deformed spring clips Figure 5 H Do not use a door with any deformed springs Lad Sakura to have it replaced Figure 5 H Examples of deformed Output Door springs Splash Guard Cleaning Use the scraper to clean accumulated paraffin on splash guard Figure 5 J during the cool down period when the Cleaning Request message Figure 5 l appears or depending on the accumulation of paraffin which could prevent efficie
66. le is shown below NOTE Cassettes are transferred to the output doors in the exact sequence they are taken out from the input magazines For example if input magazine 1 contained 14 cassettes these cassettes will be embedded and 14 blocks will be placed in output door 1 If the next ready magazine is in position 4 and contains 5 cassettes the 5 completed blocks will be found output door number 4 An empty slot will be left in the output door when a cassette or an embedded block is dropped to the chute The Embedding Run screen in Figure 4 L shows that magazine 1 was loaded at full capacity and that all 32 cassettes have been removed embedded transferred to output door 1 8 cassettes have been pulled out from input door 2 1 embedded block is already inserted to the output door 2 and 6 cassettes are on the base molds Base mold 7 is disabled for some reason Input magazine 3 in pink has completed 15 minutes warm up time and is waiting to be processed next There is no cassette in Input 4 magazine total of 14 specialty 12 biopsy 7 standard cassettes and 8 13x13 biopsy have been embedded so far The TOTALS are incremented every time a cassette is placed in a base mold They are sorted by cassette type The TOTALS are reset whenever a new user logs in the system shuts down or the count exceeds 9 999 These counts are recorded in the statistics 4 6 Feb 15 2010 paeem M
67. lt or Action 13 The reservoir copper tube 1 copper tube 2 flex tube 15 valve nozzle has dropped below acceptable operating temperature Base molds will be cleared and no more 16 E TEMPERATURE PROBLE cassettes will be embedded The paraffin system heaters 17 will be turned off and the instrument MUST be serviced Perform action s 19 before embedding can continue requested The frame heater temperature is out of acceptable operating range lt Min Max gt instrument will now shut HEATER TEMEERS TURE ERORCEM down empty input door and all output doors Contact Sakura Service Department The secondary reservoir pusher pins stager is are 14 outside acceptable operating temperature Current 18 HEATER TEMPERATURE PROBLEM magazine will be finished and base molds cleared The 20 instrument SHOULD be serviced before embedding d continues Heater A B in input door chamber has dropped below acceptable operating temperature The system will not use this input chamber until temperature is within normal range The instrument MUST be serviced before 21 chamber can be used Perform action s HEATER TEMPERATURE PROBLEM 22 Heater A B in input door chamber has risen above requested acceptable operating temperature Cassettes in the magazine will be embedded and base molds cleared The instrument SHOULD then be serviced before embedding continues During a check of the s
68. ly 4 4 inserted magazines will likely be ready for embedding for maximum efficiency The Warming Cassettes screen Figure 4 l specifically displays magazines in their 15 minute cassette warm up period In this figure as indicated by the red highlights two magazines have been loaded and will reach temperature in 13 minutes 5 seconds as shown in the upper right corner complete description of Run screen layout and color coding is given on the next page Feb 15 2010 SakuraFINETEK AutoTEC 1 5 4 p _ ERRORLOG RUNHISTORY INSTRUMENS TOTAL CASSETTES WARM UP 2 15 2010 8 00 00 2 2 CASSETTES TO THE CHUTE COOL DOWN 2 15 2010 10 00 00 PM RESERVDIR TEMPERATURE Figure 4 Run screen Warming Cassettes 64 93 C PARAFFIN LEVEL GOOD NOTE To maximize system throughput and efficiency remove any empty magazines and replace them with filled ones as they become available Operating in this manner will always guarantee that the 15 minute cassette warm up time is completed by the time the instrument robotics are ready to embed these cassettes Revised 5 19 2010 Run Screen Layout The Run Screen layout is consistent through all phases of system embedding As shown in figure 4 J the four input magazines are shown on the left while the four output doors are shown on the right reflecting the same physical view as if the operator was standing in front of the system The t
69. me and passwords Figure 3 DD NOTE The user name and passwords are not case sensitive NOTE Each entered password letter is displayed as NOTE Entries are not saved until ENTER is selected from the Passwords screen User Name Password Verify Password Operator Administrator 0 TTITTTIIT 55 TETTE Dom Figure 3 DD User Name and Password Entry screen Exporting Data The system allows sending reports and files to a flash drive The connection ports location can be seen in figure 1 B location 8 If a flash drive is connected in the USB port the data will be transferred to that drive Revised 5 19 2010 Error Log By selecting the ERROR LOG tab the ERROR LOG screen is displayed Figure 3 EE The error log displays chronological information about system errors and events that occur including a number and description run number date and time of the error or event The total numbers of selected errors are displayed inside the Total Num Error box R amp D Leb 11 2010 AutoTEC THURSDAY 07 58 08 AM Base Mold Temperature Problem 23 VERSAGENDE SENSDREN 59 Base Mold Temperature Problem 23 Base Mold Temperature Problem 1226 2010 11 29 Sensors Malfunctioning 1 26 2010 10 16 Base Mold Temperature Problem 1 26 2010 10 16 Base Mold Temperature Problem 1 26 2010 10 15 4M Base Mold Temperature Problem h 22622010 10 15
70. nd Output Doors Color Code Information Green Standard Cassettes Orange 26 x 19mm Biopsy Cassettes Specialty cassettes Orientation Core Shaved Gold 13 x 13mm Biopsy Cassettes Blue Undefined treated as a standard Output door ready to removed The number of blocks is shown below the graphic The output door is being filled Door has gray background with blocks The number of colored bars light border blocks shown 15 incremented each time one block is added Output door is empty waiting to receive blocks Door has blue background colored bars dark border Door has gray background and light border Door has gray background Output door is not present For example in Figure 4 K output doors 1 and 4 are empty and ready to accept cassettes output door 2 is full and ready to be removed while door 3 is being filled with cassettes The colored bars show the types of cassettes present OUTPUT DOORS Figure 4 K Output Door Color Codes 4 5 Embedding Cassettes Once loaded with magazines the AutoTEC will start automatically and embedding will begin after the system checks and 15 minute magazine warm up period are complete During system operation it is possible to continually replace empty magazines with loaded magazines and full output doors with empty ones The Run screen shows the progress of the embedding process for each cassette in the run An examp
71. net or control panel in particular do not use acetone on the Sakura logo or any other labeled or printed surface Use hospital grade disinfectants to disinfect exterior surfaces Use surface cleaners such as Windex or Formula 409 to clean the Control Panel touch screen Paraffin Trough Cleaning Replacement The paraffin trough is designed to catch paraffin drips from the magazines as they are heated Figure 5 A lt is located beneath the input door Paraffin drips should be cleaned from the trough after eight hours of operation Pull the trough out by grasping both ends and pulling it out Clean the trough or discard it and replace with a new paraffin trough Figure 5 A Paraffin Trough Input Doors Paraffin may accumulate at the top of the input doors Use a clean soft cloth or Kimwipes to remove the buildup This should be done at least once a day 9 1 Output Grid Sensor Cover Glass Cleaning The output grid sensor is located along the lower channel of the output doors and is protected by a cover glass See Figure 5 B Small partic f Cover Glass late on the cover glass and must be ore frequently if necessary To clean the cover glass wipe away the paraffin debris with a soft cloth soft brush or Kimwipes Figure 5 B Output Grid Sensor Cover Glass Paraffin Reservoir and Filter Cleaning It is recommended that the reservoir be cleaned eve
72. ng a column dragging it and dropping it at a new location Figure 3 QQ Feb 11 2010 Sakura RAD Lab AutoTEC THURSDAY 08 13 02 AM Summary 21712010 E 2 8 2010 H 2 9 2010 5 2 10 2010 Magazine 2 Magazine2 0 Magazine3 D Magazined 11 TOTAL CASSETTES WARM UP 2 11 2010 8 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 RESERVOIR TEMPERATURE 60 32 C PARAFFIN LEVEL GOOD Figure 3 QQ Dragging a column to a new location 3 18 By selecting the File Output button the selected run history can be transferred to a flash drive Figure 3 RR Feb 11 2010 Sakura RAD Lab AutoTEC THURSDAY 08 11 57 AM Summary Daily Daily Total Total Total 2772010 ERN oss Cassettes STD BX eee 1 CR 29 2010 E 2n 0 2010 TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 PM RESERVOIR TEMPERATURE 277C PARAFFIN LEVEL GOOD Figure 3 RR File Output Output files can be saved in any one of the three formats HTML PDF or EXCEL to help in organizing the collected data Revised 5 19 2010 Initial Start Up To start the Tissue Tek AutoTEC manually such as when using it for the first time follow this procedure 1 Use the main power switch located on the lower right side panel to turn the AutoTEC on The computer will start automatically After the software has completed
73. nt gripper stager traveling CAUTION When cleaning splash guard be careful not to bend any base mold posts NOTE The default setting for the maximum number of cassettes is 10 000 9 4 Maximum number of cassettes have been embedded Please perform the following maintenance wipe clean gripper arms and tips clean paraffin splash quards input door back panel openings and plastic flaps and paraffin trough inspect and report to Sakura Service Department if paraffin accumulation is found on the refleactive wide beam optical sensors Please touch OK to continue Figure 5 Paraffin Scraper Base Mold Posts Figure 5 J Optional Touch Screen External Keyboard and Mouse Cleaning Touch screen keyboards and other peripherals must be free of excess paraffin to ensure consistent operation Remove excess paraffin by using the scraper and use surface cleaners such as Windex Formula 4097 Use of dust covers is recommended Revised 5 19 2010 Weekly Maintenance The gripper arms should be cleaned weekly To prevent paraffin accumulation two possibilities are recommended 1 First run on Mondays Each Monday after the operator loads magazines and closes the input door on the first cycle of the day the following message will be displayed Figure 5 K WARNING Gripper arms may have excessive paraffin accumulation To maintain proper operation of the AutoTEC instrument
74. og In and to enable all the buttons and tabs Figure 3 PLEASE ENTER PASSWORD m e e sj sis OGG GE CANCEL Figure 3 Password keypad NOTE The factory default operator level PASSWORD is 100000 NOTE The factory default Administrator gt level PASSWORD is 000001 NOTE As soon as an administrator level password is set up see User Accounts on page 3 13 this factory default password should be deleted or modified Revised 5 19 2010 Following successful login the Initial MONITOR screen will appear Figure 3 J Feb 11 2010 Sakura R amp D Lab Je AutoTEC THURSDAY 07 08 56 AM jpn ERRORLOG RUNHISTORY TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 AM 2 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 5 00 00 PM RESERVOIR TEMPERATURE 47 07 C PARAFFIN LEVEL GOOD Figure 3 J MONITOR screen Touch the UTILITIES tab to access the Utilities Selection screen This screen is always accessible but not all features can be used while the AutoTEC is running Configuring System Settings Figure 3 K shows the Utilities screen as viewed by an administrator This screen gives access to various system utilities Touching an icon accesses the associated utility Icons for utilities inaccessible during AutoTEC operation display in gray Feb 11 2010 Sakura R amp D Lab Tissue Tek AutoT THURSDAY 07 25 52 AM fe
75. omponents may reach temperature before the 4 hour period has elapsed At that point the OVERRIDE TIMER button will display Figure 4 D The operator has the choice to either wait for the 4 hour cycle to complete or to touch the OVERRIDE TIMER button to force the system to the ready state In either case the OVERRIDE TIMER button disappears If the input door heaters are at temperature typically 15 to 30 minutes after warm begins the LOAD MAGAZINES button will display Because the rest of the instrument is not up to temperature do not place cassettes in the instrument until about 30 minutes prior to the end of the warm up period to avoid leaving tissues exposed to heat for too long 4 2 1030 2010 1 45 00 PM 4 30720 5 15 00 PM J GOOD A Figure 4 D Initial Run screen warm up cycle with system up to temperature It is recommended to cool down the instrument manually or automatically at the end of the day to leave instrument AC power switch on and to program a Ready Time that will ensure the system is ready when the laboratory operation begins For example if the laboratory opens at 7AM a Ready Time set at 7AM will guarantee that the warm up cycles starts at JAM and is completed by 7AM at the latest In that case after a successful log in the Monitor screen will not have any timer indication on the upper right corner and the only buttons available will be LOAD MAGAZINES LIGHT
76. ooling process CAUTION Do not move the Tissue Tek AutoTEC while paraffin is in a liquid form in the reservoir To move the AutoTEC 1 Select the UTILITIES Tab Figure 2 A Press the SHUT DOWN button and wait for the AutoTEC software and Windows operating system PC to shut down completely e Feb 02 2010 Sakura R amp D Lab B Tissue Tek AutoTEC TUESDAY 02 12 20 PM 7 UTILITIES ERRO RUN HISTORY USER ACCOUNTS READY SHUTDOWN SOFTWARE M VERSION Cool Down Mode SYSTEM SETUP CLEAN GRIPPER ARMS TOTAL CASSETTES 0 WARM UP 2 3 2010 8 00 00 2 CASSETTES TO THE CHUTE 0 COOL DOWN 2 2 2010 10 00 00 PM RESERVOIR TEMPERATURE 64 08 C PARAFFIN LEVEL GOOD Figure 2 A Utilities Tab 2 Turn off the main power switch which is located on the right side panel in the lower left corner See Figure 2 B 2 2 Hazardou Danger of shock or bi Disconnec before serv Voltage Risque et de blessi Couper le c avant l entn Figure 2 B Main Power Switch Disconnect the power cord from the outlet Raise the feet located on the bottom of the instrument by turning the nut fastened to the threaded shaft of each foot counter clockwise 9 Carefully roll the instrument on its casters to the desired location 6 Ifdesired lower the feet by turning the nut fastened to the threaded shaft of each foot clockwise CAUTION At least two p
77. orter so the top of the lid cannot be seen however as the Xpress magazine is sliding down into the ID a slight resistance should be felt near the bottom of the ID insertion as the lid slides open NOTE If the door is shut then immediately re opened the system will not sense the addition of further magazines To continue to add magazines close the door and touch CONTINUE in the prompt Figure 4 H that appears as soon as the door is closed then touch the LOAD MAGAZINES button again to insert more magazines The cassette count for a given input magazine is cleared when that magazine is removed from the door The count can also be reset immediately by logging off the system and logging in again with magazines left inside the input door Load magazine completed and input door closed Instrument will lock the input door in 24 seconds OK Figure 4 H Input Door Activity Acknowledgement 7 Securely close the input door by rotating the knob clockwise and touch CONTINUE in the on screen prompt The Warming Cassettes screen displays and the 15 minute cassette warming process begins This warm up time is required to ensure there is no solid or semi solid paraffin in the magazine that could interfere with the action of the robotic arm If magazines were previously inserted and have reached temperature the system will immediately start or continue to embed these cassettes By the time these cassettes are completed the new
78. ove all input magazines and empty all output doors then touch OK to shut down the system The Heater Controller Board is not responding Please contact Sakura Service department for further instructions Please remove all input magazines and empty all output doors then touch OK to shut down the system The TEC Board is not responding Please contact Sakura Service department for further instructions Please remove all input magazines and empty all output doors then touch OK to shut down the system INPUT DOOR INPUT DOOR BOARD NOT Input Door Stepper Board is not responding System is Eri PROBLEM RESPONDING trying to recover the error to resume current operation Instrument shuts down if OK is touched Instrument shuts down if OK is touched Instrument shuts down if OK is touched Revised 5 19 2010 CA N N Go pP CA CA oo CA CA 61 2 6 63 64 BOARD BASEMOLD PROBLEM BASEMOLD PROBLEM NON RECOVERABLE ERROR MAGAZINE 57 PROBLEM MAGAZINE NOT SEATED MAGAZINE PROBLEM SENSOR MALFUNCTION RUN ABORTED WARNING MAINTAINENCE Revised 5 19 2010 SENSOR CHECK CASSETTE IMPROPER ASSEMBLY CASSETTE LID OPENED IN FRAME CASSETTE TABS PUSHED DOWN IN FRAME AT CASSETTE IMPROPER ASSEMBLY CASSETTE TYPE CHECK Section 6 Unable to move Frame Heater Both the limit switches ar
79. per maintenance of the AutoTEC is essential to maintain the system in operative condition This section provides information on e Routine Maintenance e Magazine Maintenance and Cleaning Routine Maintenance Routine maintenance procedures include e Exterior maintenance e Paraffin Trough cleaning e Output grid sensor cover glass cleaning e Paraffin Reservoir and filter e Magazines e Output doors e Splash guards e Touch screen e Gripper Cleaning Perform these routine maintenance procedures at the following frequencies Maintenance Activity Frequency Exterior maintenance Weekly or more often if necessary Paraffin Trough After every eight hours of operation or as cleaning replacing needed Output grid sensor Daily or more frequently if necessary cover glass cleaning Paraffin Reservoir and Filter Cleaning Every two weeks or more frequently if paraffin in the reservoir appears dirty Magazine cleaning After every use Output Door inspection Inspection after every use Cleaning every and cleaning month Splash guards cleaning After 10 000 cassettes Touch screen amp optional keyboard and Mouse Monthly Gripper Weekly or more often if necessary Revised 5 19 2010 Exterior Maintenance Keep the exterior of the Tissue Tek AutoTEC cabinet free of dust and clutter Typically wiping the cabinet with a clean wet cloth will keep the instrument cabinet dust free Do not use solvents of any kind on the cabi
80. r Proprietary name for the sectionable embedding system including frames and cassettes used in the AutoTEC Revised 5 19 2010 INDEX Access Levels aatatel 3 6 7 1 550 21 Ancillary DIOGUCIS petat sette 72 AutoTEC BOCHD 1 1 MOVING ascende E 22 a 1 6 Base Molds Sole ale ERR E 4 5 cae 6 8 Buttons Clean Gripper 3 10 aj Ho PO 3 2 Istr mmelib ACCESS dates hei ennt 3 2 PEE 3 2 920 3 2 O i acted 3 2 S usa 3 2 joo ERR LC 3 2 C Clean Gripper 3 10 Components Base mold assemblies 1 4 Cassette sensor 14 COMO veas edit 1 6 Gripper assembly edet pdt at 14 Input door 5 1 3 INSIFUMENT ACCESS x 6 7 Magazines and 4 3
81. rocess 3 9 CUSTOMIZATION OF SETTINGS Touch the MONITOR tab to display the MONITOR screen COOL DOWN will be displayed at the upper left corner of the screen as seen in Figure 3 Q Feb 23 2010 Sakura Dream Lab Ay AutoTEC m Ri service TOTAL CASSETTES 0 WARM UP 2 24 2010 6 00 00 gt CASSETTES TO THE CHUTE 0 COOL DOWN 2 24 2010 12 06 00 a RESERVOIR TEMPERATURE 65 18 C PARAFFIN LEVEL GOOD Figure 3 Q Run screen Cool Down Mode count down timer in the upper right corner will indicate the remaining time until the automatic start up sequence is to begin These times can be set by an Administrator as described on page 3 8 The AutoTEC will exit the Cool Down mode and begin the 4 hour warm up process when the auto start time begins or when the operator touches the WARM UP mode button in the UTILITIES screen Software Version The Software Version screen displays the software version in use and the serial number of the AutoTEC Record this information at installation and each time the software version is updated Figure 3 R 3 10 Version Information 3 0 0 Beta IV Tissue Tek AutoTEC INSTRUMENT SERIAL NUMBER 7000 505 Sakura Finetek USA Inc 1750 W 214th St Torrance CA 90501 1 310 972 7800 1 310 972 7801 www sakuraus com TOTAL CASSETTES WARM UP 122722010 7 00 00 2 a CASSETTES TO TH
82. ry two weeks or more frequently depending on the accumulation of dirt visible in the paraffin Clean the reservoir with a pair of 12 inch forceps and pieces of gauze sponge as follows 1 Place the gauze sponges into the paraffin in the reservoir 2 With the forceps move the gauze slowly around capturing as much of the dirt particles as possible 3 Gently lift the gauze sponges out and discard them Alternatively once a month allow the reservoir to run low toward the end of the day As the paraffin starts to cool and solidify during the cool down period but while it is still pliable use a plastic scraper to remove it from the reservoir Add paraffin pellets to the reservoir to prepare for the next warming cycle 5 2 Filter Cleaning CAUTION When using solvents refer to their Material Safety Data Sheets and always observe precautions specified there Clean the filter as follows NOTE Clean the filter only after cleaning the reservoir as described above NOTE Clean the filter one at a time to prevent contaminants from getting into the paraffin dispense tube 1 Access the filter by removing the panel cover that COVers it 2 Use the tool provided to loosen the two set screws that hold down the tabs of the filter assembly One turn counterclockwise should be enough Figure 5 C 5 Revised 5 19 2010 Section 5 3 Turn the filter 907 so it can be lifted out of the 7 Gently pull the paras
83. s Setting of system parameters The four operator accessible utility screens are reviewed first in the following pages followed by the review of the three screens which can only be accessed by an administrator The ERROR LOG and RUN HISTORY screens are covered in Section 4 Operation 3 7 CUSTOMIZATION OF SETTINGS Ready Cool Down Times Operators can program automatic timers to initiate the four hour warm up routine at specific times on specific days so that the AutoTEC will be ready for the first run when the shift begins or when operators are ready to start The time entered specifies when the system will be ready for embedding plus the 15 minute magazine warming process System warm up will begin four hours before the time entered for the ON time on the Ready Cool Down Times screen Automatic timers are set on the Ready Cool Down Times screen Figure 3 L Select AUTO radial button Select READY COOL DOWN TIMES tab set a time touch in an ON TIME or OFF TIME field Use the keypad to enter a time in either 12 or 24 hour format depending on the parameter selected in the System Setup screen the entry will appear in the selected field Touch the ENTER button to transfer the time to the ON or OFF TIME field e change field to NOT USED touch in that field then touch the NOT USED button After all times have been entered check that they are correct and then touch the SAVE AND EXIT button
84. sacas nter scott tmt e radit anao pce o este 4 1 4 2 5 CARE OF THE INSTRUMENT OI osos dodenus cem dU ose MM ELIMINA UE 5 1 Routine Maintenance eect ied ehh aa aia a maia 5 1 Parattin Reservoir and Filter Cleaning 35i tee a 5 2 Magazine Cleaning aaa aita lalluliisu an 5 4 Output Door Inspections and 5 4 Splash Guard ChE AI ING bd 5 4 Optional Touch Screen External Keyboard and Mouse Cleaning 5 4 Gripper Weekly Maintenance wis eee idu e pa eats 5 5 Revised 5 19 2010 TABLE CONTENTS Section Page 6 TROUBLESHOOTING ENOR LIEU MI LIE E 6 1 Error GOdeS and 855 6 6 2 Error Recovery 6 7 7 SERVICE AND REPLACEMENT PARTS 7 1 Replacement ouai datei aa 7 1 APPENDIX A CEOS tie t A 1 APPENDIX B MAC TERRE m ee ea B 1 APPENDIX C
85. sample of the current selection Select SAVE AND EXIT to save the changes Select EXIT to abort the changes Revised 5 19 2010 DATE and TIME Setup For current DATE setting select the DATE down arrow to activate the calendar screen Select the correct date Figure 3 Y Mar 10 2010 Sakura R amp D LaB ox e 2 i 32 Tissue Tek AutoTEC weonespay 091526 es ERRORLOG RUN HISTORY LANGUAGE English ALARM VOLUME row 71 TEST ALARM ALARM SOUND ALARM2 j TEST END END PROCESS SOUND End Of Process 1 71 RESTORE DEFAULTS un Mon Tue We n Sa INSTITUTION A SAVE AND gt 3 1 E oda EXIT TOTAL CASSETTES WARM UP 3 10 2010 6 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 3 10 2010 8 00 00 PM 2 RESERVOIR TEMPERATURE 64 36 C PARAFFIN LEVEL GOOD Figure 3 Y System Setup screen Date For current TIME setting highlight one of the three time fields for Hours Minutes and Seconds use the UP or DOWN arrow to increment or decrement the highlighted selection Figure 3 Z Mar 10 2010 Sakura R amp D LaB ox e 2 f 92 Tissue Tek AutoTEC WEDNESDAY 09 16 02 AM p la isi US LANGUAGE English 7 ALARM VOLUME LOW Y TEST ALARM ALARM SOUND ALARM2 71 TEST END END PROCESS SOUND End Of Process 1 RESTORE DEFAULTS DATE AND TIME Tuesday March 09 2010 8 29 18 PM Al gj INSTITUTION Sakura R amp D LaB SAVE AND EDIT
86. sen LT E TT 002 Axis Stall In The V Axis LogiD EriD ShortDescription Date 003 Axis Stall In The Input Door Axis 005 Gripper Failed To Close 007 Block Not Removed From Base Mold 008 Axis Stall In The U Axis 009 Axis Stall In The W Axis 010 Axis Stall In The X Axis 011 Asis Stall In The Y Axis 012 Axis Stall In The Z Axis 13 Heater Temperature Problem 021 Heater Temperature Problem 022 Heater Temperature Problem TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 RESERVOIR TEMPERATURE 49 3 C PARAFFIN LEVEL GOOD Figure 3 GG By User Select the User pull down arrow to display the pull down menu Scroll to the selection Figure 3 HH xx Febii 2010 Tissue Tek AutoTEC 7573 11 Feb 2010 v 11 Feb 2010 x LogiD EID ShortDescription Service TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 10 00 00 RESERVOIR TEMPERATURE 49 59 C PARAFFIN LEVEL GOOD Figure 3 HH NOTE Built in user is created to automatically log events that happen when no user is logged in 3 15 CUSTOMIZATION OF SETTINGS e The ALL button will display a listing of ALL errors that are logged in the instrument from the very first one registered till the last one When this button is pressed the From and To dates will change accordingly and the ALL button will also c
87. solenoid valve and nozzle over the cassette already placed into the heated base mold It then stages presses down with a tamper the Sectionable Cassette to lower it through the frame until the cassette bottom is pressed against the base mold surface The stager dispenses an even flow of molten paraffin to the correct volume Since the temperature and flow are controlled bubble production is minimized however tiny to small bubbles in the formed block are not unusual Figure 1 H shows paraffin being dispensed into a base mold Figure 1 H Stager Dispensing Paraffin Two paraffin dispensing methods are available Standard or Pre fill configurable in Service Mode Standard method dispenses a fixed amount of paraffin in a single shot Pre fill method dispenses the fixed amount of paraffin in two separate shots the first shot is dispensed into the base mold before the cassette is placed and the second shot is dispensed after the cassette is staged Please contact sakura if the Pre fill method is needed When paraffin is dispensed the base mold switches to its cooling mode Once the block has solidified the stager moves away and the gripper comes back to demold the block and to place it in one of the empty positions of the output doors Revised 5 19 2010 Output Doors Figure 1 1 1 i EL t m We tt m SS Figure 1 Output Doors The four output doors interlocked to prevent operator access while
88. t doors and all magazines from the input doors 2 Acknowledge instructions by touching OK in the error message pop up 3 Wait for the no input signal to display on the screen indicating that the software has shut down properly 4 Use the main power switch located on the lower right side panel to turn the AutoTEC off 5 Look for and remove any Paraform Cassettes that may have dropped below the gripper and or been placed in the recovery chute 6 Wait for 15 seconds Use the main power switch located on the lower right side panel to turn the AutoTEC on The application software will load automatically T From the Log In screen use the screen keyboard to enter user name and password 8 Touch the LOG IN button The initial run screen displays with the system start timer visible at upper right 9 The system will home after warming up and move any Paraform cassettes remaining in the base molds to Output Door 1 After that the T E C self test will follow When it appears at the lower left of the screen touch the OVERRIDE TIMER button to override the system start timer Embedding will now resume Revised 5 19 2010 Section 6 If error messages continue to occur after implementing the error recovery procedure contact Sakura Finetek for further assistance See Section 7 Service and Replacement Parts for further information about how to contact Sakura Finetek Instrument Access NOTE Do not access the interior
89. t 1 8 gt disabled due to block not removed Error Log FROM BASE MOLD The instrument has experienced a non recoverable error with the U V W X Y Z Input Door Stepper Please remove all input magazines and empty all output doors instrument will now shut down Contact Sakura Service Department for further instructions INSTRUMENT EXPERIENCES 3 CONSECUTIVE STALLS WITHIN 20 MINUTES Contact Sakura Service Department Touch OK Touch ABORT to open input door and check magazine 4 Touch RESUME to continue with the current cycle User has 60 seconds to respond to message Input Magazine s n not seated properly Please reseat these magazines Touch OK to open input door now Check magazine for cassettes NOTE Removing magazine will cause the magazine to warm up for 15 minutes Do not remove any other magazines UNABLE TO HOLD CASSETTE FROM INPUT DOOR The following sensors are not function properly Failed sensor check Touch OK Please contact Sakura Service Department for further instructions Chute message displayed Please open the front door and close cassette lid on base mold Standard Then touch RESUME to continue current process OR touch ABORT if more time is needed To prevent base molds and cassettes from being damaged instrument will continue embedding and clear all base molds except base mold X and stop embedding in 180 seconds OPEN LID DETECTED Touch ABOR
90. tandard biopsy orientation core biopsy shaved biopsy and 13 X 13 biopsy versions The AutoTEC program mode where the system is not operating and is cooling to room temperature The paraffin is cooling or in a solid form during this time The process of surrounding tissue specimens in paraffin blocks See Paraffin Paraform component into which a cassette is inserted used by robot to handle cassettes and embedded blocks Frames are color coded and have a slanted front surface to write or imprint specimen identification Solenoid actuated device mounted on the Y axis that transports each frame cassette assembly through the system The process of examining describing and trimming tissue specimens to proper size then loading the specimens into cassettes 32 cassette and 20 cassette magazines that hold Paraform cassette assemblies for loading in the AutoTEC Input Magazines 15 minute period required to bring Input Magazines to temperature before embedding can begin When closed covers input and output doors when open provides work space for magazines and output doors Interlocked doors each equipped with a spring loaded matrix that firmly holds cassettes in place after processing yet enables easy access and removal of cassettes Output doors are removable and can be used as transport trays for embedded cassettes The embedding medium used by the AutoTEC Only Tissue Tek Paraform Processing Embedding Medium Formul
91. the loading process the Monitor screen will display Figure 4 A Feblt 2010 Sakura R amp D Lab E JeTisueTek AutoTEC T rva TOTAL CASSETTES WARM UP 2 11 2010 8 00 00 2 CASSETTES TO THE CHUTE 0 COOL DOWN 2711 2010 5 00 00 PM RESERVOIR TEMPERATURE Figure 4 A System Start Up screen 47 06 C PARAFFIN LEVEL GOOD 2 Select the LOG IN button to access the ENTER PASSWORD screen Figure 4 B PLEASE PASSWORD Oo Figure 4 B Log In screen Revised 5 19 2010 Section 4 NOTE Refer to page 3 14 User Accounts to set up the passwords 3 Use the keypad display to type in the password 4 Select ENTER when done 9 Monitor screen is displayed with a LOG OFF button Figure 4 C 11 2010 Sakura R amp D Lab 3 lt H SETissueXek AutoTEC sa ff TOTAL CASSETTES 0 WARM UP 2 11 2010 8 00 00 2 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 5 00 00 PM RESERVOIR TEMPERATURE 47 07 C PARAFFIN LEVEL GOOD Figure 4 C Typical Operating Seguence A typical operating seguence for the Tissue Tek AutoTEC is as follows e Warm p System perform automatic self checks e Operation Preliminary checks Verify the proper placement of Paraform cassettes into the magazines Insert magazines inside the input door Embed Paraform Cassettes Remove output doors containing embedded
92. there will be no old message left to clear the output door Remove all specimen blocks from the output door or use the output door as transport tray Revised 5 19 2010 remove one or more doors 1 Push the small red button located above the upper right corner of the output door to release the latch Figure 4 M 2 Grip the door handle and firmly pull the door away from instrument 3 To replace place the bottom edge of the empty door in the channel and push the door towards the instrument until it latches with a distinct click click is required for the instrument to recognize the door is in place Figure 4 M Removing an Output Door Add Paraffin If the paraffin level falls below a certain point an on screen message and audible alarm will prompt the user to add paraffin to the reservoir At this point the system has sufficient paraffin to embed at least 128 more cassettes or four full magazines NOTE While the system is running only molten paraffin should be added while it is in its warming cycle pellets may be added to the reservoir NOTE Use only Tissue Tek Paraform Processing Embedding Medium Formula 3 product code 7052 NOTE When the paraffin reservoir temperature drops below an acceptable range the instrument will restart after the user acknowledges the displayed message Revised 5 19 2010 Section 4 Cassette Recovery Chute In certain instances an improperly assembled Paraform
93. umber of inoperative base molds has exceeded three the system will still run but the throughput will decrease Also DO NOT attempt to load more magazines Figure 6 F Revised 5 19 2010 If the operator continues another message will prompt the operator to remove any block that may be present in the disabled base mold when the cycle is complete Figure 6 C Inspect the Base mold s 8 for blocks by opening X front door Click to move stager aut af BM X8 to access cassette Move Stager J Section 6 cannot be resolved do not use this magazine position and call for service Cassette Lid Open A cassette with the lid not closed properly has been detected in base mold 1 Standard Open the front door Remove the cassette from base mold 1 Figure 6 G If base mold 8 is disabled then an additional button MOVE STAGER will appear on the screen Figure 6 Please pick up block from BM 8 immediately by opening front door Touch ABORT the stager will automatically move back over BM 8 in 1 76 Seconds RESUME Figure 6 H Select this button to move the stager so that the operator has access to the block base mold 8 Once MOVE STAGER is selected the operator will be prompted to remove the block from base mold 8 Figure 6 H Magazine Problem System is trying to move the cassettes down in magazine 1 Please stand by X Figure 6 Magazine Problem magazine problem will
94. und between frames can be an indications of not properly closed cassette lid or cassette is pushed through the frame Open the magazine lid and investigate the causes of the gap and correct if necessary Ensure that cassettes are properly inserted in the frame with lids securely closed d Make sure the magazine retainer can slide one space above the topmost frame in the magazine The retainer is seen in the magazine shown in Figure 4 F e Ensure that the magazine lid is closed Touch the LOAD MAGAZINES button A message will appear indicating that the system is preparing the input door to be opened When the input door is ready to be opened Okay to open input door will appear in the message area and a two minute timer will display at upper right If the door is not opened in time the system will assume that access is not required and return to the state it was in prior to the LOAD MAGAZINES request Open the input door by turning the knob counter clockwise Figure 4 Figure 4 G Loading a Magazine 4 3 9 Hold the magazine upright against paper towels or other absorbent material to allow excess paraffin to drain off the magazine before placing it in the AutoTEC 6 Insert the loaded magazine s into the input door and press each of them down as far as possible NOTE After the magazine is inserted verify the magazine lid is popped open for the Standard magazine The Xpress magazine is sh
95. v DF READY SHUTDOWN SOFTWARE USER ACCOUNTS VERSION SYSTEM SETUP SHUT DOWN Cool Down Mode CLEAN GRIPPER ARMS TOTAL CASSETTES WARM UP 2 11 2010 8 00 00 2 CASSETTES TO THE CHUTE 0 COOL DOWN 2 11 2010 5 00 00 PM RESERVOIR TEMPERATURE CC PARAFFIN LEVEL GOOD Figure 3 K Utilities Selection screen Administrator Revised 5 19 2010 Section 3 Administrator level access allows access to all utility functions Operator level access is restricted to the following functions some being on a view only basis These are Full Access e UTILITIES o Ready Cool Down Times o Shut Down o Cool Down Mode Warm Up Mode o Clean Gripper Arms e Error Log Run History View Only e UTILITIES o User Accounts o Software Version o system Setup NOTE Icons unavailable to operator level users or inaccessible during AutoTEC operation are displayed in gray NOTE To avoid data corruption some of the parameters cannot be changed while the AutoTEC is in operating mode running or ready to run These restricted parameters are Setting the completion of the four hour warm up routine automatically at specific times ready times on specific days Setting of the automatic cool down at specific times on specific days Override of the programmed ready and cool down times for scheduled days off such as holidays or vacation days Creation of user names and passwords Setting of user defined name
96. ystem heaters reservoir secondary reservoir copper tube 1 copper tube 2 flex tube valve nozzle drip tray ID A ID B thermal Perform action s E fuse was discovered to be functioning improperly requested The instrument will not run until this problem is fixed Please contact maintenance for further assistance 24 The tamper gripper heater temperature is out of bod dc HEATER TEMPERATURE PROBLEM acceptable operating range Min Max base molds will 25 requested be cleared no more cassette will be embedded The drip tray heater temperature is out of acceptable operating range lt Min Max gt instrument will continue Perform action s A ERTE VET embedding as normal Contact Sakura Service requested Department OUTPUT DOOR lt 1 4 gt IS Please replace Output Door lt 1 4 gt then touch OK to Touch OK NOT PRESENT resume instrument operation 27 REQUEST ADM Operation WILL NOT continue until Output Door 1 is in OUTPUT DOOR 1 place and empty Once the Output Door has been Touch OK replaced touch OK to resume instrument operation Operation WILL NOT continue until Output Door 2 is in i ORT ACE place and empty Once the Output Door has been Touch OK replaced touch OK to resume operation Operation WILL NOT continue until Output Door 3 is in 27 REQUEST A place and empty Once the Output Door has been Touch OK replaced touch OK to resume oper

Operating Manual AutoTEC (ENG)

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