380_385 Serv Manual
Contents
1. Parts required Light Trap Assembly 4PL0890 0580 Open the detector s side panel using the access key as shown The light trap is located behind the PMT module as indicated 1566 27401 Varian ELSD Service Manual 59 Using an Allen key loosen the grub screw as indicated and carefully pull out the optical light trap as shown 1566 27401 Varian ELSD Service Manual 60 Replacing The Optical Heater When required Failure of optical heater or optical heater fuse Tools required Allen keys Screwdriver Parts required Optics Heater 4PL0890 0595 discharge ESD In order to prevent damage always use ESD Electronic boards and components are sensitive to electronic N protection when handling electronic boards and components ATTENTION Open the detector s side panel using the access key as shown Using an Allen key remove the optics heater plate from the optical block assembly as shown Disconnect the optics heater cable as highlighted from the main PCB board Replacement of the optics heater fuse as highlighted is also recommended when the heater is replaced 6 27401 61 Varian ELSD Service Manual Removing amp Cleaning The Prism Assembly When required Contaminated prism High baseline offset Tools required Allen keys Lint free optical lens tissue Lens clean2. Exhaust Waste tube Glass Sleeve Neb Chamber Vapour Sensor Varian 385 LC Main PCB 110v 240v 110v 240v Jt ASS ARA eee n d i 1566 27401 Part No PL0890 0110 PL0890 0240 PLO890 1110 PLO890 1240 PL0890 0300 PL0890 0305 PLO890 0310 PLO890 0315 PL0890 0320 PL0890 0325 PL0890 0330 PL0890 0335 PLO890 0340 PL0890 0345 PL0890 0350 PLO890 0355 PL0890 0360 PL0890 0365 PL0890 0370 PL0890 0375 PL0890 0380 PL0890 0390 PL0890 0395 PL0890 0400 PL0890 0405 PL0890 0410 PL0890 0415 PL0890 0420 PL0890 0425 PL0890 0430 PLO890 0435 PLO890 0440 PLO890 0445 PLO890 0450 PLO890 0455 PL0890 0460 PL0890 0465 PL0890 0470 PL0890 0475 PL0890 0480 PL0890 0485 PL0890 0490 PLO890 0495 PL0890 0500 PL0890 0505 PLO890 0510 PLO890 0515 Varian ELSD Service Manual Varian 380 LC Power Supply Gasl Inlet Frit 101m MFC Inlet Plug Varian 385 LC Power Supply FFKM O ring 19 30x2 40 FFKM Neb O Ring 6 0x1 0 PTFE Nebuliser Chamber Nebuliser Waste Tube P Trap Diffusor Viton O ring 19 30x2 40 Viton O ring 0 7x1 0 Viton O ring 15 30x2 40 Light Trap Assembly Prism Assembly Optics Heater O ring 21 95x1 78 O ring Nitrile Prism Optics Prism Peltier Assembly Mains Inlet Fuse Pk 5 Serial USB Adapter 1566 27401 PL0890 0520 PL0890 0525 PL0890 0530 PL0890 0535 PL0890 0540 PL0890 0545 PL0890 0550 PL0890 0555 PL0890 0560 PL0890 0565 PL0890 0570 PL0890 0575 PL0890 0580 PL0890 0585 PL
3. File Edit Settings Phone Transfers Help Send Text File lt lt TMON188 TARG neceive Text File Uersi E Copyright C Arcom P Tet Hle Send Binary File Receive Binary File 6166 gt P UNE Pause Resume Stop ST x File Name Directories 9041 1 1 HEX c swtest mainctrl j BEN Cancel 3041 171 HEX c EA RELEAS 1 RTF PRODUC 1 Network gt ELS2100 2 SWTEST 2 MAINCTRL Following CH Append LF List Files of Type Drives VW Strip LF All files _ Ei Vlitaniumissued Figure 5 Selecting the HEX file 1 Terminal Untitled File Edit Settings Phone Transfers Help Type for command list 2 Program Flash device erased ok Awaiting hex file transfer Figure 6 Downloading the Program 3166 27401 74 Varian ELSD Service Manual l Terminal Untitled File Edit Settings Phone Transfers Help Type for command list gt Program Flash device erased ok Awaiting hex file transfer cece eee ee ene eee eee Imt EM Flash memory updated ok d Figure 7 Programming the EPROM 6 27401 75 Varian ELSD Service Manual Programming Device Identity Tools required Parts required N A 6 27401 TERMINAL exe or Hyperterminal exe supplied with Windows ELSD Device ID TRM program Serial cable supplied with instrument ae ned id Eain ub cO C Ey 1 Close down the control software 2 Co
4. Reading FAULTY Replace Evaporator Assembly see RTD page 32 24 Fan failure on cooled evaporator Replace Cooled Evaporator Assembly see page 43 25 Bridge current outside of normal range Replace Cooled Evaporator Assembly see page 43 6 27401 12 Varian ELSD Service Manual General Problems Fault Problems Possible Cause s Remedy Baseline noise The particle plume is not sufficiently dried in the evaporator tube e Increase the temperature of the evaporator by 10 intervals until the noise is acceptable Baseline noise Poor nebulisation of solvent e Increase the gas flow rate e Decrease the nebulisation temperature Baseline noise Pump pulsations especially in microbore applications where low flow rates are used e Usea pulse free pump e Increase the back pressure on the pump by fitting a back pressure regulator between the pump and the injection valve e Use a pulse dampener directly after the pump in the system Baseline spikes 1 Particulate matter in the gas supply 2 Column shedding 3 Poor nebulisation e Filter the incoming gas or change the supply e Replace column or fit an inline filter with a 0 2um membrane filter directly after the column e Nebuliser temperature may be too high and solvent may be boiling reduce nebuliser temperature Low sensitivity and baseline noise Diffuser saturated with solvent e Stop the eluent flow and increase the evapor
5. carefully before attempting to service the instrument In addition be sure to observe all signs and pictographs that are specifically defined as follows WARNING ATTENTION NOTE 1566 27401 WARNING The warning sign denotes a hazard It calls attention to a procedure practice which if not correctly done or adhered to could result in severe injury or damage or destruction of the instrument Please do not proceed beyond a warning sign until the indicated conditions are fully understood and met ATTENTION The attention sign denotes relevant information Read this information first before proceeding it will be helpful or necessary to complete the task NOTE The note sign denotes additional information It provides the user with advice and suggestions to facilitate the operation of the instrument Varian ELSD Service Manual WARNING ATTENTION NOTE WARNING ATTENTION A NOTE A ATTENTION 6 27401 SAFETY The following procedures require opening the main cover of the detector Always ensure that the detector is disconnected from the mains power when the side panel is opened The Varian ELSDhas a safety bar at the power input socket that prevents the detector s side panel being opened when the power cable is still connected To disconnect the detector from the mains supply line unplug the power cord When working with solvents please observe appropriate safety procedures for example safety gogg
6. of the A D interface Serial RS232 Connector The Varian ELS detector is fitted with a standard RS232 DTE DCE 3 wire serial interface The serial RS232 connector provides a 24bit 10 or 40Hz digital output for connection to a PC running a data acquisition package e g Galaxie Chromatography software If controlling the instrument from a PC a free serial port is required A USB port on the PC can be used but a USB to Serial adapter is required PL0860 0620 The Varian ELS detector is also fitted with a service connector located below the RS232 port for flash upgrading firmware see pg 71 3166 27401 5 Varian ELSD Service Manual Connection to a Varian Star 800 Module Interface Box The Star 800 Module Interface Box MIB provides analogue to digital signal conversion ADC Connections are made by connecting the analogue output cable to one of the analogue signal input ports on the middle right side of the Star 800 MIB For more information about connecting your Varian ELS detector to a Star 800 MIB refer to your Star 800 MIB documentation Control I O connector The Varian ELS detector can be connected to auxiliary equipment such as an autosampler or pump via the 15pin I O connector The I O connector can be configured in several ways to allow on board timetable events to be triggered or to remotely auto zero and shutdown the detector The ELS detector is equipped with 2 contact closures normally open for stopping the opera
7. or error then the gas flow is set to a minimum flow of 1 2SLM for 15minutes before the gas manifold valve is closed This minimum blanket gas is enough to nebuliser and evacuate solvent should the instrument default to STANDBY mode with solvent still flowing firmware 1 1 8 or later If the Instrument is left in STANDBY mode for longer than 15mintes gas flow to the unit is stopped to minimize gas usage Itis strongly recommend that the pump stop from the I O connector of the ATTENTION Varian ELSD is connected to the HPLC pump to prevent solvent flooding of the detector should an error occur Run Mode The RUN mode is the detector s normal operational mode In this mode the instrument controlled at the set temperatures and gas flow and the system is fully operational During heating or cooling the instrument will display NOT READY to show the system has not reached the set conditions When the instrument has reached the set conditions and equilibrated READY will be displayed and the instrument is ready for use USING THE INSTRUMENT IN NOT READY STATUS MAY NOT PRODUCE CONSISTENT RESULTS ACCORDING TO THE SET PARAMETERS AS THE SYSTEM IS NOT EQUILIBRATED NOTE 3166 27401 11 Varian ELSD Service Manual TROUBLESHOOTING If a problem is encountered Polymer Laboratories advises a system test be performed via the Varian ELSDPC control software to ensure that the detector is working correctly If there is an error or fault
8. please refer to the following table If you follow the recommended course of action and the result is not satisfactory then please direct the matter to Polymer Laboratories or your local distributor Instrument Error Codes Error Code Description Suggestions 10 Air Temp lt 10 C Inside Enclosure Environmental Temp Too Cold 11 Air Temp gt 40 C Temp Inside Enclosure Environmental Temp Too Hot 12 On Board Sensor Heater Failed Vapour Sensor Needs Replacing see page 27 13 Rear Panel Sensor Heater Failed Vapour Sensor Needs Replacing see page 27 14 Leak Detected Inside Unit Solvent Vapour Detected Stop Pump 15 Liquid Leak Sensor Has Detected Liquid In Liquid In The Base Of The Unit Stop Drip Tray Pump And Investigate 16 Fan Driver IC Thermal Shutdown Fault With The Circuitry Wires Fan 17 Fan Stopped Fault With The Circuitry Wires Fan see page 30 18 Nebuliser Temperature Exceeded Threshold Faulty Thermocouple Or Heater After Stabilizing Control 19 Evaporator Temperature Exceeded Threshold Faulty Thermocouple Or Heater After Stabilizing Control 20 Light Source Error Replace Light Source Assembly see page 38 21 Evaporator Gas Flow Rate Exceeded Check Operating Head Pressure Threshold After Stabilizing Otherwise Faulty Mass Flow Controller see page 37 22 Invalid Nebuliser Temp Reading FAULTY Replace Nebuliser Heater Assembly RTD see page 22 23 Invalid Evaporator Temp
9. that provides active cooling and heating to the evaporator tube A drain tube connecting the nebuliser chamber to the optical assembly diverts condensed vapour out through the front of the instrument This central section of the instrument also contains the mass flow controller and gas valve which are identical to the 380 LC model Temperature Controlled Evaporator Assembly 3166 27401 43 Varian ELSD Service Manual Removing And Cleaning The Varian 385 LC Evaporator Cartridge Diffuser When required Loss or reduction in sensitivity Noisy Baseline Tools required Allen Keys Parts required Evaporator Cartridge PL0890 0475 Ensure that the detector has cooled down before proceeding ATTENTION Open the detector s side panel using the access key as shown Remove the nebuliser chamber as described in Replacing the nebuliser chamber pg 24 1166 27401 44 Varian ELSD Service Manual Remove the evaporator cartridge from the end of the evaporation tube as shown Place the evaporation cartridge in a beaker of dilute acid and sonicate for ca 30 mins Rinse the evaporator cartridge with water then acetone Replace the cartridge into the evaporator tube 1166 27401 Varian ELSD Service Manual 45 Replacing The Varian 385 LC Cooled Evaporator Assembly When required Failure or damage to the Evaporator Heater Assembly Evaporation tube become irrever
10. the inside side panel to allow removal of the front panel Remove the front panel by lifting and pulling backwards to release the four locating screws from the bulkhead 1566 27401 Varian ELSD Service Manual 16 Overview of Nebuliser Assembly The nebuliser assembly is based around a nebuliser chamber white PTFE block into which the nebuliser drain tubes gas tubes and evaporation tube are connected Access to the nebuliser section requires the removal of the front panel see page 15 Nebuliser Nebuliser Gas Port Nebuliser Mount Heater Evaporator Gas Port Nebuliser Chamber Jl d Liquid Drain tube tt Vini 6 27401 17 Varian ELSD Service Manual Removing The Nebuliser When required If Nebuliser becomes blocked Tools required Allen keys Spanners Parts required ELSD Nebuliser 0890 0390 Ensure that the detector has cooled down before proceeding Ensure that all gas and solvent connections are disconnected ATTENTION prior to proceeding Open the detector and remove the front panel as described in Removing the front panel pg 15 Disconnect the nebuliser tubing from the inlet bulkhead Disconnect the gas inlet as highlighted 6 27401 18 Varian ELSD Service Manual Loosen the nebuliser mounting plate by loosening the top screw as highlighted VERY carefully remove the neb
11. using the access key 6 27401 64 Varian ELSD Service Manual Remove the three screws as indicated and unplug all connections Remove the six screws from the rear of the instrument as highlighted Carefully lift the board from its mountings Replace the new PCB and re connect all the cables Check Firmware on replacement board is correct version for ELSD model Qu ve m am m i a TE C He 1566 27401 Varian ELSD Service Manual 65 Replacing The Power Supply When required Defective or Failed Power Supply Tools required Screwdriver Allen Keys Parts required Varian 380 LC Power Supply PL0890 0520 Varian 385 LC Power Supply PL0890 0535 Electronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use ESD protection when handling electronic boards and components NOTE Open the detector s side panel using the access key Locate the power supply on the main PCB Unplug the two connectors highlighted 6 27401 66 Varian ELSD Service Manual Remove the ferrite from the group of wires as shown Disconnect the wires from the power supply as shown Remove the power supply from its mounting by unscrewing the four screws as shown 3166 27401 Varian ELSD Service Manual 67 Remove the power
12. 0890 0595 PL0890 0615 PL0890 0620 PL0890 0625 PL0890 0630 PL0890 0635 PL0860 0620 Varian ELSD Service Manual 81 INSTRUMENT SPECIFICATIONS LED 480nm Light Source Detector Photomultiplier Tube Temperature Range Varian 380 LC Evaporator Nebuliser Varian 385 LC Evaporator Nebuliser OFF 25 to 120 C OFF 30 to 90 C OFF 10 to 80 C OFF 25 to 90 C Nebuliser Gas Typical inlet Pressure 60 100PSI 100PSI Maximum Inlet Pressure Gas Flow Rate Up to 3 25SLM 0 2 to 5ml min Eluent Flow Rate 0 1V FSD Analogue Output 24bit 10Hz amp 40HZ via serial port Digital Output Communications Operation Power Requirements Serial I O RS232 Contact Closure TTL Remote Start Input Graphical Vacuum Fluorescent display and keypad Windows based PC control 90 120V AC or 220 250V AC 50 60Hz 200x450x415mm Dimensions wxdxh Weight Varian 380 LC 11Kg 13Kg Varian 385 LC 1566 27401 82 Varian ELSD Service Manual CLEANING amp DECONTAMINATION PROCEDURES Switch off and disconnect power cord from instrument before cleaning or decontamination Allow any hot parts to cool before WARNING proceeding Cleaning The exterior of the instrument should be cleaned by wiping down with a soft cloth moistened with dilute detergent solution followed by wiping down with a cloth moistened with deionised water Ensure that no moisture enters the instrument Allow instrument t
13. 2 SENDT O 16 41 44 Figure 10 Successful Communication with Get Data command 1166 27401 Varian ELSD Service Manual 1 Terminal ELSDDE 1 TRM 001B0000000001B6 Figure 11 Device ID displayed 6 27401 79 Varian ELSD Service Manual IDENTIFYING PARTS AND MATERIALS Description 380 LC Evaporative Light Scattering Detector 380 LC Evaporative Light Scattering Detector 385 LC Evaporative Light Scattering Detector 385 LC Evaporative Light Scattering Detector Analogue Output Cable Gas Inlet Tube 2m Exhaust Hose PVC 2 0m Waste Hose Tygon 7cm Solvent Waste Container 500ml RS232 Communication Cable Varian ELSD Mains Lead UK Varian ELSD Mains Lead EURO Varian ELSD Mains Lead US I O cable Remote Start Cable Varian ELSD Operation Manual Varian ELSD Service Manual Varian ELSD IQ OQ Workbook Varian ELSD Control Software Varian ELSD Dimension Software Varian ELSD Driver for Chemstation ELSD Nebuliser Leak Sensor Assembly Mass Flow Controller Photo Detector Assembly ELSD Light Source Fan Assembly Front White LED Assembly Gas Inlet Assembly AR20 Regulator Assembly Front Outlet Tube ELSD Key Front Panel Moulding Solvent Inlet Connector 1 16th ELSD Grey keypad Varian 380 LC Display Window Varian 385 LC Display Window Varian 380 LC Main PCB Evaporator Cartridge Evaporator Heater Assembly No Cartridge Varian Neb Mount Assembly Neb Chamber Assembly Exhaust Tube
14. A max with a protective earth connection Gas Nitrogen 98 purity or better and filtered to 0 2um Notes gt gt Air can onlybe used for non flammable solvents The mass flow controller is not calibrated for use with gases other than Air or Nitrogen For operation with other inert gases contact Polymer Laboratories for advice Gas flow up to 3 25 SLM 60 psi 25 C Pressure operating range 60 100 psi 4 6 7 bar Maximum Pressure 100 psi 6 7 bar During normal operation the carrier solvent is evaporated as it passes through the instrument and must be extracted safely at the rear of the unit The exhaust from the instrument 13mm ID PVC tubing must be extracted to a fume hood or similar solvent disposal unit If the extraction tube provided with the instrument is to be extended it is recommended that the diameter of the extension is increased to at least 50mm 2 diameter tubing so the extraction quality is not inhibited 1566 27401 Varian ELSD Service Manual Unpacking the detector Care has been taken to ensure that the instrument should be received in proper condition The packing and protection are designed for normal hazards of road rail or air transit Any damage to the container or instrument should be reported immediately to your local distributor or to Varian It is recommended that the shipping container be kept if possible for re shipment or return to a service centre Examine the shipping carton for v
15. SLMs maybe necessary to control the baseline noise Evaporator Temperature The effects of altering the evaporator temperature tend to be less dramatic than changing the gas flow although the temperature must be high enough to evaporate the solvent and to sufficiently dry the particle plume without having a detrimental effect on the sample being studied The Varian ELSD can be operated at sub ambient temperatures to improve the detection of semi volatile compounds In aqueous solvents baseline noise may become too excessive at temperatures lt 15 C However for organic solvents the baseline can be controlled down to 10 C provided the gas flow is increased to compensate Nebuliser Temperature The nebuliser temperature is the parameter requiring least adjustment In the majority of cases the nebuliser temperature Is set to the temperature of the chromatography system However increasing this temperature can improve instrument performance by increasing the efficiency of nebulisation by reducing the viscosity and surface tension of incoming solvent Setting the nebuliser temperature too high may result in a deterioration of detector performance due to solvent boiling in the nebuliser giving rise to increased noise on the baseline due to spiking Optimisation Procedure Set the system at the minimum evaporator and nebuliser temperatures and a gas flow rate of 1 6 SLM Make an initial injection of the test component and monitor the signal respons
16. Service Manual FOR Varian 380 LC amp 385 LC EVAPORATIVE LIGHT SCATTERING DETECTOR Version 1 0 January 2008 PL0890 0360 en Polymer Laboratories Polymer Laboratories Ltd Now a part of Varian Inc Essex Road Church Stretton Shropshire SY6 6AX UK Tel 44 0 1694 723581 Fax 44 0 1694 722171 Service Tel 44 0 1694 724333 Polymer Laboratories Varian Inc Amherst Fields Research Park 160 Old Farm Road Amherst MA 01002 USA Tel 1 413 253 9554 Fax 1 413 253 2476 Polymer Laboratories Varian B V Herculesweg 8 4339 PL Middelburg The Netherlands Tel 31 0 118 671500 Fax 31 0 118 671502 Polymer Laboratories Varian Belgium NV SA Mechelsesteenweg 362 2860 St Katelijne Waver Belgium Tel 32 0 15 556460 Fax 32 0 15 556186 Polymer Laboratories Varian Deutschland GmbH Alsfelder Strafe 6 D 64289 Darmstadt Germany Tel 49 0 6151 7030 Fax 49 0 6151 703237 Varian S A 7 Avenue des Tropiques Z A Courtaboeuf B P 12 F 91941 Les Ulis Cedex France Tel 33 1 6986 3838 Fax 33 1 6928 2308 Document Revision History 6 27401 Revision Date Section Changes Approval Changed Draft 24 Jan 2008 All Originated SMB DO NOT ISSUE THIS PAGE CONTENTS INTRODUCTION GAGN 1 SAFETY Snnt do aia v D E n 2 INSTALLATION riada 3 Site Preparation Check ista 3 UNPACKING Me delec ET onbres 4 Packing lia 4 POWER CONNECTIONS sssmeivounarinirco e
17. achieved Disconnect the in line flow meter and reconnect the regulator to the nebuliser The IQ OQ procedure must now be preformed to ensure the detector is performing as specified see PMT section pg 51 A quality control test must be performed following nebuliser removal to ensure detector performance Appendices 3 and 4 Varian ELSD Operation Manual NOTE 6 27401 21 Varian ELSD Service Manual Replacing The Nebuliser Heater Assembly When required Failure of Nebuliser heater Tools required Allen keys Spanners Parts required Varian Neb mount assembly PL0890 0485 Ensure that the detector has cooled down before proceeding Ensure that all gas and solvent connections are disconnected ATTENTION prior to proceeding Open the detector and remove the front panel as described in Removing the front panel pg 15 Remove the nebuliser as described in Removing the nebuliser pg 18 Disconnect the gas tubing from the nebuliser mount and Remove the three mounting screws with an Allen key as shown 1166 27401 Varian ELSD Service Manual 22 Disconnect the two Nebuliser Heater Assembly cables from the main control board NEB HTR amp NEB and remove the nebuliser heater assembly Ensure that the earthing cable is connected to the nebuliser heater assembly before switching on the instrument ATTENTION 1166 27401 Varian ELSD Service Ma
18. ain adjustment 6 27401 53 Varian ELSD Service Manual To achieve the desired sensitivity follow the IQ OQ procedure and record the response obtained for glucose If the ELSD does not achieve the specified glucose response then calculate the necessary offset value using the equation Original Offset tel shown Glucose Response mV The OFFSET value should not exceed 140mV 6 27401 54 Varian ELSD Service Manual Replacing The LED Light Source Assembly When required Faulty LED Tools required Allen keys Lint free optical lens tissue Parts required ELSD Light Source PL0890 0410 Eye damage may result from directly viewing the light produced by the LED used in this instrument Always turn off the power supply before removing the light source WARNING Open the detector s side panel using the access key as shown Disconnect the gold and blue connectors from the light source assembly Loosen the grub screw on the light source assembly 6 27401 55 Varian ELSD Service Manual Carefully remove the light source assembly Attach the replacement light source assembly Make sure the assembly is pushed up against the focusing collar Re connect the gold and blue connectors Tighten the grub screw 1166 27401 Varian ELSD Service Manual 56 Cleaning The Optical Lens Assembly When required Contamination within th
19. atible with older ELS NOTE Device Identifier To allow the control of multiple ELS detectors from a single PC Varian ELSDwith firmware versions 21 5 5 have programmable device identification The default value is set at 1 with a maximum of 254 The device identity can be configured as shown in section P rogramming Device Identity 6 27401 70 Varian ELSD Service Manual Upgrading Firmware Tools required TERMINAL exe or Hyperterminal exe supplied with Windows Varian ELSD firmware file e g 9041 2204 HEX Serial cable supplied with instrument Parts required N A 1 Close down the control software and turn off the instrument 2 Close down any software currently connected to the unit and turn off the instrument 3 Atthe rear of the detector move the switch located below the serial connector to the up position 4 Run the HexTerm or Terminal exe program 5 Select the Varian ELS detector from the menu bar and the correct communication port 6 Turn on the instrument and check that the message Bootloader v904211 is shown 7 Browse to select the new firmware e g A 9061_2204 hex 8 Select the download icon to upgrade the EPROM with the new firmware During transfer the auto zero light on the detector front panel will turn red and flash 9 On successful completion of the programming a message will appear and all three lights on the front panel will turn green 10 Exit the HexTerm program a
20. ator temperature to 50 C above the current set temperature Increase the flow rate to 2 8SLM and wait 15mins Large Baseline offset 1 Inefficient evaporation 2 High concentration of non volatile buffer or stabiliser 3 Contaminated diffuser e Increase the evaporator temperature and or gas flow e Use a lower concentration of Stabiliser unstabilised solvent or a more volatile buffer e Perform steam clean Section 4 2 Operation s manual Peak tailing Eluent particles lingering in the optical chamber e Increase gas flow rate Instrument Fails to zero Offset too high or output unstable e Ensure the instrument is in RUN mode e Refer to local distributor or Polymer Laboratories No power 1 Mains lead not connected 2 Fuse failure e X Attach mains lead to socket and inlet on rear of instrument e Replace fuse No response completely flat baseline 1 Data acquisition leads not connected 2 Light source inactive 3 Instrument in STANDBY mode e Ensure connectors to computer or integrator are sound e Check LED e Select RUN mode Temperature error as soon as instrument powered on Temperature probe fault or disconnected e Check RTD connections Display not on but power connected blue glow ON Instrument in service mode e Rear panel switch set to RUN 1566 27401 Varian ELSD Service Manual 13 REPAIRING THE VARIAN ELSD Ovemiewof Mai
21. bly Tools required Allen Keys Spanners Screwdriver Parts required Leak Sensor Assembly 0860 0395 Open the detector s side panel using the access key as shown Ensure there is no liquid in the leak sensor well located underneath the nebuliser chamber Dry with tissue if necessary Remove the two screws on the leak sensor assembly Disconnect the cable that connects the leak sensor assembly to the main control board Replace the leak sensor assembly and reconnect the cable If necessary replace the leak sensor cable assembly 3166 27401 Varian ELSD Service Manual 26 Replacing The Vapour Sensors When required Faulty Vapour sensor s Tools required Access key Small screwdriver Parts required Vapour Sensor PL0890 0510 The Varian ELSD is fitted with a differential vapour sensor system The external sensor is located at the rear of the instrument the internal sensor is located on the main control NOTE board Open the detector s side panel using the access key as shown Locate the external vapour sensor on the rear of the instrument 6 27401 27 Varian ELSD Service Manual Carefully remove the sensor by unplugging the sensor from the back of the instrument The internal vapour sensor is located on the main PCB as shown Carefully remove the sensor by unplugging the sensor from the main
22. board as shown LAM j E 4 4 d Lin LE NE Iul Se X a ie Aj z Once the vapour sensor has been removed check that the locating pins are not damaged 7 by removing the cover as E ID TAN Nn shown d gt 1166 27401 Varian ELSD Service Manual Ensure that the copper pins in each hole are close together to ensure a good contact will be made with the replacement sensor s pins If necessary move the pins with a small screwdriver 1566 27401 Varian ELSD Service Manual 29 Replacing The Fan Assembly When required When faulty or noisy Tools required Allen keys Parts required Fan Assembly 4PL0890 0415 Open the detector s side panel using the access key as shown Disconnect the fan cable as shown FAN Remove the two screws of the fan assembly For the 380 LC Insert the replacement fan with airflow from the back to the front of the unit i e the silver label is not visible Connect the fan cable to the main control board 1166 27401 Varian ELSD Service Manual Remove the two screws of the fan assembly For the 385 LC Insert the replacement fan with airflow from the frontto the back of the unit i e the silver label is visible as shown Connect the fan cable to the main control board The fan assembly on the Varian 385 LC must be installed in the opposite direction to th
23. e e Connect the waste tube to the waste outlet at the front of the detector and position the other end in a waste collection bottle e The solvent waste container supplied with the detector can be sited on the bench in front of the instrument Alternative waste collection vessels can be used if required but the bottom of the waste tube must be below the height of the waste outlet from the instrument The waste tube must NOT be submerged in the liquid e Connect the exhaust hose between the exhaust outlet and a fume hood e Connect the instrument to the data recorder computer chart recorder etc using the cable provided One output socket is provided giving a signal in the 0 1V range e Connect the column outlet to the eluent inlet at the front of the unit using a short length 10cm of tubing 1 16 OD 0 010 ID and a 1 16 Valco fitting e Turn on the source gas to a pressure of about 60 100 psi The gas will not flow though the detector until the instrument in set to RUN mode e Switch on the ELSD and select method XXX e Start heating by selecting RUN mode using the arrow keys on the front keypad e When the unit has equilibrated the baseline should be checked to ensure that it is acceptable for the experiment At this point with no liquid flowing into the instrument the noise should be no more than 0 3mV peak peak This verifies that the gas supply is clean and dry Any spikes in the baseline are usually indicative of particu
24. e With each subsequent injection increase the 6 27401 9 Varian ELSD Service Manual nebuliser and evaporator temperatures by 10 C whilst reducing the gas flow rate until optimum sensitivity is achieved In some cases especially for volatile samples it has been found that maximizing the nebuliser temperature permits a lower evaporator temperature and thus an increase in sensitivity 1566 27401 Varian ELSD Service Manual 10 OVERVIEW OF DETECTOR S OPERATION MODES The Varian ELSD can be operated in two modes STANDBY and RUN as described below To display the current mode and or select a new mode highlight the MODE function on the instrument display The current mode will now be displayed on the screen Using the A V keys scroll up or down until the desired option is displayed The instrument acknowledges the command by displaying the mode of operation in the top right hand corner of the screen Standby Mode The STANDBY mode is the ground state of the Varian ELSD instrument which is initiated automatically after power on In this mode of operation the heaters are off and the gas manifold valve is closed The STANDBY mode enables the user to set up the operational parameters gas flow nebuliser and evaporator temperatures before switching the unit into RUN mode The instrument will default to STANDBY mode should an error occur on the instrument When the instrument is switched from RUN mode to STANDBY mode following a command
25. e 380 LC as shown to prevent the power supply overheatin ATTENTION PP J 1166 27401 Varian ELSD Service Manual 3 Overview of Varian 380 LC Evaporator Assembly The Varian 380 LC evaporator assembly comprises a heated tube and drain tube connecting the nebuliser chamber to the optical assembly as shown This section of the instrument also contains the Mass flow controller and gas valve Access to this part of the instrument can be gained by opening the side panel but the front panel may need removing for certain service operations 77 Evaporator Tube Opical Block 6 Nebuliser Chamber o Liquid Drain Tube 6 27401 32 Varian ELSD Service Manual Removing And Cleaning The Evaporator Cartridge Diffuser When required Loss or reduction in sensitivity Noisy Baseline Tools required Allen Keys Parts required Evaporator Cartridge PL0890 0475 Ensure that the detector has cooled down before proceeding ATTENTION Open the detector s side panel using the access key as shown Remove the nebuliser chamber as described in Replacing the nebuliser chamber pg 24 6 27401 33 Varian ELSD Service Manual Remove the evaporator cartridge from the end of the evaporation tube as shown Place the evaporation cartridge in a beaker of dilute acid and sonicate for ca 30 mins Rinse the evaporator cartridge with water then acetone Replace the cartridge into the e
26. e optical assembly offset too high Tools required Allen keys Lint free optical lens tissue Parts required See factory for details on required parts Open the detector and remove the front panel as described in Removing the front panel pg 15 Loosen the grub screw shown to release the Light Source Lens Tube on the optical assembly Pull the Light Source Lens Tube out of the optical block The Light Source Lens O ring and spacer are held in place by a Wave Ring spring Use a small screwdriver to release the spring see later Remove the lens for cleaning using lint free optical lens tissue Refit the lens and reassemble referring to the optical assembly diagram to orientate the lens correctly 6 27401 57 Varian ELSD Service Manual To clean the Photodetector PMT assembly first remove the assembly as described in Removing the Photodetector PMT Assembly pg 51 The lenses are held in place by a Wave Ring spring see below Release the spring using a small screwdriver The Wave Ring spring can then be removed as shown Remove and clean the lenses using lint free optical lens tissue Reassemble referring to the optical assembly diagram to get the correct lens orientation 1566 27401 Varian ELSD Service Manual 58 Replacing The Optical Light Trap When required High Baseline Offset Noisy baseline Tools required Allen keys Screwdriver
27. eal two screws which hold the valve in place 6 27401 39 Varian ELSD Service Manual Remove the two screws to take out and replace the gas valve 1166 27401 Varian ELSD Service Manual 40 Replacing The Nebuliser Gas Regulator When required Faulty gas regulator Tools required Allen keys Parts required AR20 Regulator Assembly PL0890 0430 Open the detector s side panel using the access key as shown Disconnect the gas supply to the instrument Locate the nebuliser gas regulator situated behind the optical assembly as shown 1166 27401 Varian ELSD Service Manual 4 Remove the light trap to allow easier access to the regulator See section Replacing the Optical light trap pg 59 for details of how to remove the light trap In order to remove the regulator undo these two screws Detach all gas lines into the regulator and extract the regulator Insert the new regulator re fasten the screws reconnect the gas tubing and re attach the light trap Follow the procedure outlined in Installing the nebuliser pg 20 in order to configure the correct gas flow to the nebuliser 1566 27401 Varian ELSD Service Manual 42 Overview of Varian 385 LC Evaporator Assembly The evaporator assembly of the Varian 385 LC comprises a temperature controlled evaporator tube section Peltier unit
28. er Parts required Prism assembly PL0890 0585 Open the detector s side panel using the access key as shown Remove the two screws located at the bottom as shown and top of the inside panel Remove the side panel to gain access the rear of the optical assembly Using an Allen key undo the screw to remove the prism assembly clip as shown 1166 27401 Varian ELSD Service Manual 62 Very carefully slide out the prism assembly from the optical assembly The prism assembly can be replaced or cleaned using lint free optical tissue and cleaner 3166 27401 Varian ELSD Service Manual 63 Electronics The electronics comprise three main components 1 Detector s Main Control PCB 2 Power Supply 3 Cooled Evaporator assembly Varian 385 LC only Replacing The Main Control PCB When required Defective Main Control PCB Tools required Screwdriver Allen keys Parts required Varian 380 Main Control PCB PL0890 0470 Varian 385 Main Control PCB PL0890 0515 Heater PCB Fuses There are 3 fuses fitted to the main control PCB one to protect each of the heater circuits FH 1 amp FH 3 H 1A FF 250V FH 2 TR5 2A T 250V Electronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use ESD protection when handling electronic boards and components NOTE Open the detector s side panel
29. isible signs of exterior damage Unpack the instrument and examine it for transit damage Check that all items on the packing list are included Notify your local distributor or Polymer Laboratories Varian of any damage or missing items Packing list Varian ELS Detector Operation Manual Control Software SMB1 Mains leads UK EUR USA RS232 data cable Analogue output Cable Gas Tubing 2m PVC Exhaust hose 2m Tygon SE200 waste hose 7cm 500ml Solvent waste container Power Connections e Before connecting the power cable ensure the instrument voltage rating matches your local power supply e Use only a supply with protective grounding e The correct fuses should be installed e For 115V AC or 230V AC use two 250V H 2A T fuses THIS UNIT IS DOUBLE FUSED ATTENTION RISK OF FIRE REPLACE FUSES AS MARKED WARNING If the voltage rating and fuses are correct for your power source connect the power cable Gas Connection The instrument should be supplied with clean dry nitrogen gas at a minimum head pressure of 60psi A 4mm push in connector is provided at the rear of the instrument for a convenient connection to the gas source To prevent against unnecessary gas usage an automatic but controlled gas shut off valve is integrated into the gas inlet manifold This will only allow gas to pass into the instrument when the instrument is operating Should the instrument default to a standby mode the gas valve will close af
30. late matter or water in the gas supply e Turn on the eluent flow and allow the system to stabilize for approximately 15 minutes THE ELUENT FLOW CAN BE TURNED ON BEFORE THE INSTRUMENT HAS REACHED EQUILIBRIUM IF THE ELUENT IS NOT EFFICIENTLY NEBULISED IT WILL EXIT THE INSTRUMENT FROM THE NEBULISER DRAIN TUBE NOTE e Again check the baseline noise as it should not have increased significantly and should be 1mV Typically the baseline noise is 0 3mV peak peak If the baseline noise is excessive then one of the following may be taking place e poor evaporation due to gas flow being too low e poor evaporation due to evaporation temperature being too low e poor nebulisation due to temperature being too high and solvent boiling Any of these problems will cause the baseline to shift upwards as the amount of scattered light within the instrument has increased If the baseline continues to show unacceptable noise even when the evaporation conditions have 3166 27401 7 Varian ELSD Service Manual been improved and the solvent does not contain non volatile species then please refer to Chapter 5 Troubleshooting Where noise and all other conditions are acceptable the instrument is ready to begin work NON VOLATILE BUFFERS ARE NOT COMPATIBLE WITH THE ELSD NOTE Storing the Instrument If the instrument is to be stored or not used for a period of time it is recommended to follow the procedure outlined below e Allow the instru
31. les gloves and protective clothing especially when toxic or hazardous solvents are used Electronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use ESD protection when handling electronic boards and components The LED light source is a Class 1 LED product Temporary discomfort may result from directly viewing the light produced by this source Do not look into the beam Make sure that only fuses with the required rated current and of the specified type super fast fast time delay etc are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Any adjustment maintenance and repair of the opened instrument under voltage should be avoided as much as possible When inevitable this should only be carried out by a skilled person who is aware of the hazards involved Use of a mains isolating transformer is recommended Read and understand the Varian ELSD user manual before undertaking any service operations on this instrument Varian ELSD Service Manual Site Preparation Check List Environmental Conditions BO Power E Gas Supply Extraction Requirements INSTALLATION Temperature 15 to 35 C 59 to 86 F At constant temperature Avoid positioning in direct sunlight Humidity 10 80 USA and Japan 115V AC 10 50 60 Hz 2A max with a protective earth connection Europe 230V AC 10 50 60 Hz 2
32. ment to cool to ambient temperature in STANDBY mode with the gas supply still connected e Tip the instrument forwards and empty the P trap contents through the front waste tube i e into the bottle e Pour 10 to 20mls of acetone into the exhaust tube to flush out the P trap collecting the waste in the waste bottle at the front of the instrument e Again tip the instrument forwards to drain the p trap e Disconnect the waste bottle e Using the gas supply blow nitrogen gas through the exhaust to evaporate any remaining acetone in the p trap Cover the waste tube with tissue paper to collect any acetone residue e Plug the exhaust and waste tubes with the plastic caps provided e Plug the solvent inlet 3166 27401 8 Varian ELSD Service Manual OPTIMISING DETECTOR PERFORMANCE General Considerations The Varian ELSD instrument should be thought of as a detector like any other designed for liquid chromatography The main distinguishing feature is the ability to evaporate the solvent from the column eluent Therefore normal system set up precautions should be remembered when starting to use the instrument Any solvent intended for use with the ELSD should be fully miscible with any previously used in the liquid chromatograph if there is any uncertainty a mutually miscible solvent should be run through the system as an intermediate liquid The sample loop should also be flushed with miscible solvent where necessary The intended eluent sh
33. n Assenrbly To allow easy access to the internal components of the Varian ELSD the left side panel folds outwards The lock is located at the rear of the instrument which can be opened using the access key part 0890 0440 Light Source Evaporator Tube See page 33 Varian 380 LC See page 44 Varian 385 LC See paae 56 Detector module See bade 52 MNebuliser Assembly Mass Flow Controller See paae 18 See paae 38 Main board See naae 65 Fan assembly See pnaae 31 Power supply See page 67 6 27401 14 Varian ELSD Service Manual Removing The Front Panel When required For majority of internal repairs Tools required Allen keys Varian ELSD Enclosure Access Key 0890 0440 Parts required Ensure that the detector is disconnected from the mains power before proceeding WARNING To prevent personal injury the power cable must be removed from the instrument before opening the detector Do not connect the power cable to the detector while the covers are removed WARNING Use the Access key to open the lock located at the rear of the instrument Fold down the left side panel of the detector That FING IH LE JEL FF Disconnect the nebuliser tubing and cables from the inside of the front panel TH A Quz LJAN Ea Ui M e E Li viden en we pa pa ee ar z a Nes CDL i J 6 27401 15 Varian ELSD Service Manual Remove screws located at the top and bottom of
34. nd turn off the instrument dd a A IIA C 11 On the back of the Varian ELS detector move the switch located below the serial connector to the down position E 12 Switch the detector back on and check the version of firmware in Galaxie by choosing the status option in the Varian ELS detector control window 7 T E X JU Son 6 27401 71 Varian ELSD Service Manual E Terminal Uintithed Fle Edt Settings Phone Trarsferz Help Figure 1 Terminal program Lommunications Baud Rate C110 300 600 C 1200 2400 4800 C 9600 19200 Data Bits Stop Bits es CB C7 68 E C15 C2 Cancel Parity Elow Control Connector None C Xon Xoff C Odd C Hardware C Mark C Space 7 Parity Check Carrier Detect Figure 2 Communications dialog 6 27401 72 Varian ELSD Service Manual l Terminal Untitled File Edit Settings Phone Transfers Help lt lt PIC17C MINI MONITOR gt gt Version 1 6 3 Copyright c Polymer Laboratories Ltd 2882 Type for command list gt Figure 3 Successful Communications 41 Terminal Untitled E In xj File Edit Settings Phone Transfers Help a Type for command list gt Program Flash device erased ok Awaiting hex file transfer Figure 4 Initializing the Programming 1566 27401 73 Varian ELSD Service Manual E Terminal Untitled
35. nnect the serial cable to the RS232 port on the rear of the instrument 3 Start the ELSD Device ID TRM program Figure 8 and configure the Communications in the Settings menu figure 9 4 Check the instrument successfully communicates with the terminal program by selecting Get Data figure 10 5 Now obtain the identity of the ELSD by selecting GET ID The following value should be obtained 0015000000000 B6 where Xis the device ID figure 11 amp Tosetthe ELSD device ID to 1 select the SETID1 7 Check the device ID has been set to the correct value by selecting GET ID The output should read 001B0000000001B6 76 Varian ELSD Service Manual Zl Terminal ELSDDE 1 TRM File Edit Settings Phone Transfers Help 4 L Gebaa sm SETID2 eve GetlatDaa SETI 14 49 24 Figure 8 ELSD Device ID Terminal program Communications Baud Rate C110 300 C600 C 1200 2400 4800 9600 19200 Data Bits Stop Bits es CB C7 68 E C15 C2 Cancel Parity Elow Control Connector None C Xon Xoff Odd Hardware C Even None C Mark C Space Parity Check Carrier Detect Figure 9 Communications dialog 6 27401 TI Varian ELSD Service Manual Terminal ELSDDE 1 TRM File Edit Settings Phone Transfers Help 018018 WISHM T EFG 3 F9D LL X 4 4 A Getbata sen SETID
36. nual Replacing The Nebuliser Chamber When required Blocked or contaminated Nebuliser Tools required Allen keys Spanners Parts required PTFE Nebuliser Chamber PL0890 0490 Ensure that the detector has cooled down before proceeding Ensure that all gas and solvent connections are disconnected ATTENTION prior to proceeding Open the detector and remove the front panel as described in Removing the front panel pg 15 Remove the nebuliser heater assembly as described in Replacing the nebuliser heater assembly pg 22 The nebuliser and heater assembly can be removed as one section as shown in diagram 6 27401 Varian ELSD Service Manual 24 Disconnect the black tubing to the nebuliser chamber Using Allen keys remove the two screws connecting the nebuliser chamber to the side panel be sure to support the nebuliser chamber Whilst holding the nebuliser chamber carefully remove the drain tube from the nebuliser chamber Carefully pull the evaporator tube and drain tube out of the nebuliser chamber as shown Replace with the new chamber and re connect the gas tubing Ensure that the earthing cable is connected to the nebuliser heater assembly before switching on the instrument ATTENTION 1166 27401 Varian ELSD Service Manual Replacing The Solvent Leak Assembly When required Faulty Solvent leak sensor assem
37. o dry off completely before reconnecting power Decontamination The operator should wear appropriate personal protective equipment for this operation gloves safety glasses lab coat and respirator if level of hazard has been risk assessed to be sufficiently high Disconnect the instrument completely and remove it to a fume cupboard if necessary Empty the P trap before moving the instrument See Storing the Instrument pg 8 Open the cover of the instrument to see if any solvent has contaminated the interior Excess quantities of liquid spilt on or inside the instrument should be mopped up using absorbent cloths followed by repeated wiping down with soft cloths moistened with Acetone or deionised water as appropriate to the spilt solvent until the last traces of the hazardous liquid have been removed If liquid has collected in the drip tray around the leak sensor then be very careful not to damage or displace the sensor bead whilst mopping up Identify and if possible correct source of the leak Inspect cabling parts and surfaces to determine whether any damage has occurred If in doubt contact Varian Service Dept for assistance Allow the interior and exterior of the instrument to dry out completely before closing the cover and reconnecting power Dispose of contaminated waste appropriately Version 2 0 83 627401 Varian ELSD Service Manual V1 0 doc
38. osition and guarantee the alignment is correct Position the mounting bracket underneath the evaporator assembly Whilst pushing the bracket and evaporator assembly backwards towards the side wall of the ELSD tighten the evaporator assembly screws shown in yellow This will lock the position of the assembly to the bracket and prevent rotation of the assembly 1566 27401 Varian ELSD Service Manual 48 Finally tighten the bracket to the sidewall of the ELSD using the screws highlighted in green 6 27401 49 Varian ELSD Service Manual Overview of Optical Assembly The optical assembly of the Varian ELSD has been designed for easy maintenance however there may be occasions where the optical surfaces need cleaning The diagram below shows the major components of the optical assembly Optics Focus Collar LED assembly Prism Assembly Lens Tube Rubber Plug Window PMT assembly Light Trap 6 27401 50 Varian ELSD Service Manual Removing The Photodetector PMT Assembl When required Faulty photomultiplier Tools required Allen keys Lint free optical lens tissue Parts required Photo Detector Assembly PL0890 0405 Electronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use ESD protection when handling electronic boards and components ATTENTION Open the detector s side panel u
39. ould be thoroughly degassed contain no non volatile salts or material and should be fully compatible with the column s All connections should be made with zero dead volume fittings and tubing with an I D 0 010 The Varian ELSD requires nitrogen purity gt 98 capable of generating 60 100psi inlet pressure If in house nitrogen is not available then we recommend the use of a nitrogen generator giving a constant uninterrupted supply of high purity gas Air can be used with non flammable solvent systems The eluent of choice should be fully volatile under the chosen detector parameters any non volatilised eluent will increase baseline noise and reduce sensitivity Optimisation Parameters Gas Flow An increase in gas flow rate causes a decrease in signal response Lower gas flow rates are more favourable since less gas is consumed and a better sensitivity is achieved However there comes a point at which this benefit is counterbalanced by the increase in baseline noise due to inefficient evaporation of the eluent In general gas flow rates of 1 0 2 0SLM tend to be a reasonable compromise between baseline stability and high reproducible response for eluent flow rates gt 0 5ml min Reducing the eluent flow allows a reduction in the gas flow rate to maintain the optimum particle stream concentration resulting in greater sensitivity When operating the Varian 385 LC at sub ambient temperatures in aqueous solvents gas flow values of gt 2 0
40. our Sensors xxi ec pet ere Du Sut dion ate Rute a bead e te bos eaa etd a AE 27 Replacing The Fan ASS 0 EEE EE EE N 30 Overview of Varian 380 LC Evaporator Assembly errem nennen nnn nnns 32 Removing And Cleaning The Evaporator Cartridge Diffuser rrsrrrrorrrvrrrrrvrrrorvorrenrenrenrrvrrnenrenrenrrnrrnrrvrrnesnennenn 33 Replacing The Evaporator Heater Assembly Evaporation Tube senes 35 Reach Ihe MaSS FOW CONTONE cussssstiedes tututatad deoa Deaths dedu fed eta Ona uds Pass utut 37 Replacing ne Gas INE Plug ASSEMDIY csdosdiaei es obe Ha OR a b ee ata di eu ie omes 38 Replacing The Gas Inlet Assembly sss nnn nnn nnn nnne tnnt nn tnnt inan 39 Replacing The Nebuliser Gas Regulator nnne nnne nnn tnnt nnns 41 Overview of Varian 385 LC Evaporator Assembly eren nennen nennen nnns 43 Removing And Cleaning The Varian 385 LC Evaporator Cartridge Diffuser occococcccoccnoconoccnconconconcnnonnoos 44 Replacing The Varian 385 LC Cooled Evaporator Assembly sees 46 Overview of OpBcal ASMA 50 Removing The Photodetector PMT Assembly ae 51 Adjusting Photodetector PMT Sensitivity Lunn acini aat dd ii 53 Replacing The LED Light Source ASSEMDIY ccccccsscscescesssssssssessessseessessessesaesessesseseeseeseeseusausassaseeseesaesansansas 55 Cleaning The Optical Lens AS SOMO ae 57 Replacing TE Optical LIONE TAD sastosteresteste Raten tidie trgfe
41. quired Mass Flow Controller PL0890 0400 Open the detector s side panel using the access key Unplug the two black gas lines from either end of the Mass flow controller Disconnect the Mass flow controller cable from the top the MFC Remove the four screws located underneath the base of the instrument to remove the mass flow controller assembly Insert the replacement mass flow controller and reconnect the gas lines and control cable 1166 27401 Varian ELSD Service Manual Replacing The Gas Inlet Plug Assembly When required Every 12 months or when filter becomes blocked Tools required Spanner Parts required Gas Inlet assembly 4PL0890 0425 a MOM a Nb MON Ho F 1h A RJ i ie al hi The gas inlet plug assembly is located at y the rear of instrument as shown rod Using a spanner unscrew the assembly and remove as shown 1166 27401 Varian ELSD Service Manual Replacing The Gas Inlet Assembly When required Faulty Gas Inlet Valve Tools required Spanner Parts required Gas Inlet Assembly 4PL0890 0425 Gas inlet Frit 10um 4PL0890 0525 Open the detector s side panel using the access key as shown Locate the gas valve as shown and disconnect the red crimp connectors and all gas tubing Locate the gas inlet plug assembly as shown and peel off the sticker to rev
42. rn e n Cru a cd FD CE KR AGERE DET RE v CRX Gas ConnectUOnh seran boda SC Puri HD D EOD FD E EIC E E bc E Fc E EH E RE Eu FN HD E FIA Connection auod oio rion nO GEAR 5 EXIracuoi sicot MM n E EH E ER E P ED RERO ER EE A d Av 5 Data Connect 5 Analoge Signal ORIG Otitis a 5 EAR COME A A a NN 5 Connection to a Varian Star 800 Module Interface BOX sese 6 Go elt A I 6 Installing me Detecci n 7 Storing ame SUMEN aaa 8 OPTIMISING DETECTOR PERFORMANCE iman dais 9 General Considerauons iria ana 9 Ophmisabon Parameters auos endo and cran nno lo rco o Fc o D 9 GaS m em 9 EVaporator Femperatule 5 vta A Ue be on sa CR P p Su E DLE 9 Nebullser TENNE ada dias 9 Op misation Pr cedule osinndron doen nanaqna ro V EROR ERO UD REDE On Rn Do inf 9 OVERVIEW OF DETECTOR S OPERATION MODES 1 1 rrr nnn nnn nnns 11 Standby MOG sinas A 11 RUN A E HV AX RAE DT EV ELE ET E PAPER 11 TROUBLESHOOTING nario RR CURATE 12 Instrument Error OG OS ioco arvo cci viso i ERE Ev ceo UR C ae Ced ve EQ Ri 12 General Probe iia 13 REPAIRING THE VARIAN EES Di 14 Overview OF M Asset 14 Ieroviag Ie FOM Pause 15 Overview of Nebuliser Assembly is adn ncn oc d oc Fc o c a rnc cra 17 rn TI A 18 PSA Re NeDUIISST SS 20 Replacing The Nebuliser Heater Assembly sese 22 Replacind ThedNebullser nata dt 24 Replacing The Solvent Leak Assembly eee 26 Replacing The Vap
43. sdotndiaen Gen TEE E 59 Replacnd The ODUCSL Hale aa oe decem arce lucri dnte mo Dc Ui ts 61 Removing amp Cleaning The Prism ASSEMDIY ccccssscssssssssssssssrssressesessrsseeseessessrsstssrseeeseeseeseesarssnsetseteseeseeses 62 EE NS aaa 64 Replacing The Mahn CO PCB vr ed 64 Replacing The PoWel SUNN isi s o bad epis roce dot Qut koc ia 66 FINNW ale assiduis avara d ee 69 Device de a Gula 70 PINNE 71 PSN PENG EN 76 IDENTIFYING PARTS AND MATERIALS curia 80 INSTRUMENT SPECIFICATIONS taco ive hia ru Or E CR ri vu ds 82 CLEANING amp DECONTAMINATION PROCEDURES 1 1 rernm nnn nnn 03 3 0 T coa 03 PENN NE 83 INTRODUCTION The combined Varian ELSD Service Manual is designed for use by personnel who have had training and are experienced in servicing this type of equipment Whilst the ELSD has been designed for easy repair there are potential hazards associated with servicing this instrument We strongly recommend that our service engineers perform all servicing It is understood that the purchaser must assume all risk in the use of this manual for the purpose of performing service upon the instrument it covers There are no user serviceable parts in the Varian ELSD Varian Polymer Laboratories strongly recommends the use of original Varian spare parts only otherwise we do not guarantee any specification or liability It is strongly recommended that the operator read the entire manual
44. sibly contaminated Tools required Allen keys Spanner Parts required Varian 385 LC Peltier Assembly PL0890 0630 If this procedure is not followed correctly the cooled evaporator assembly may be damaged NOTE Open the detector s side panel using the access key as shown Remove the evaporator tube from the nebuliser chamber as described in Replacing the nebuliser chamber pg 24 Do not discard evaporator cartridge 1166 27401 Varian ELSD Service Manual 46 Disconnect the following connectors from the cooled evaporator assembly Carefully remove the evaporator assembly from the mounting bracket by unscrewing the two nuts shown in red Support the evaporator assembly and remove the mounting bracket by the three screws as shown The evaporator assembly can now be removed from the optical block as shown Be careful not to exert excess force on the optical block or evaporator assembly 3166 27401 47 Varian ELSD Service Manual To replace the cooled evaporator assembly carefully connect the ends of evaporator tube into the optical block and nebuliser chamber as shown Reconnect the electrical and gas connections DO NOT TIGHTEN ANY SCREWS AT THIS POINT AS THIS CAN DAMAGE THE ASSEMBLY Having positioned the evaporator assembly tighten the nebuliser chamber screws shown in red This will lock the ends of the assembly into p
45. sing the access key as shown Locate the PMT module on the optical assembly as shown 6 27401 51 Varian ELSD Service Manual Disconnect all 3 cables from the PMT assembly Loosen the grub screw on the PMT assembly and remove the black rubber plug as shown Carefully remove the PMT assembly from the optical block Attach the replacement PMT assembly making sure all three cables are re connected Tighten the Grub screw and re insert the black rubber plug The rubber plug must be inserted into the PMT assembly otherwise the detector will not perform correctly NOTE 6 27401 52 Varian ELSD Service Manual Adjusting P hotodetector P MT Sensitivity When required Following PMT or Nebuliser change Tools required Parts required IQ OQ Manual Glucose Test solution Electronic boards and components are sensitive to electronic discharge ESD In order to prevent damage always use ESD protection when handling electronic boards and components ATTENTION owitch on the ELSD and record the offset value displayed on the front screen Locate the PMT module on the optical assembly as shown Remove the cover of the PMT module to expose the internal PCB The PMT gain is adjusted by turning the screws on the two blue components The right component is the coarse gain adjustment The left component is the fine g
46. supply Insert the new power supply and re connect the cables Ensure the correct wires are connected to correct positions on the green terminal block The pair of black amp yellow wires connect as follows Black position 3 Yellow position 6 as shown The pair of red amp black wires connect as follows Red position 1 Black position 2 The single black wire screen connects as follows Large Black position 3 1566 27401 Varian ELSD Service Manual The pair of pink amp blue wires connect as shown Pink position 4 Blue position 5 The group of 4 wires connect as shown Red position 1 Black position 2 Orange position 4 Blue position 5 When all of the wires are connected the green block should appear as shown Firmware The Varian 380 LC amp 385 LC contain control firmware that can be flash upgraded as described in section Upgrading Firmware pg 65 The Varian 380 LC also contains additional firmware on the cooled evaporator assembly to control the Peltier unit This firmware can only be flash upgraded at the factory The current firmware versions February 2008 are Varian 380 LC v22 0 2 Varian 385 LC v22 0 4 6 27401 69 Varian ELSD Service Manual A 24bit 10Hz digital output via the serial port is available with firmware versions gt 1 5 5 N Firmware versions 22 0 2 amp 22 04 are not comp
47. ter 15 minutes Varian ELSD Service Manual NOTE THE GAS INLET VALVE WILL BE CLOSED WHEN THE INSTRUMENT IS FIRST POWERED ON AND WILL ONLY OPEN ONCE THE INSTRUMENT IS SET TO RUN MODE Fluid Connection The eluent from the chromatography system is connected to the front of the instrument via the low dead volume Valco bulkhead connector provided USE ONLY VALCO FITTINGS ATTENTION The liquid inlet port is connected directly to the nebuliser by a short length 130mm of capillary tube giving a delay volume from port to nebuliser tip of 5ul Extraction The Varian ELSD is provided with tubing for venting the exhaust gases and vapors and so does not need to be placed in a fume cupboard Instead the exhaust hose provided must be attached to the rear of the unit and vented to a fume hood or other disposal unit Ensure the exhaust hose has an upward slope from the Varian ELSD so that any condensed solvent is collected in the waste bottle at the front of the unit and to prevent it accumulating in the tubing THE EXHAUST MUST BE EXTRACTED TO A SUITABLE FUME EXTRACTION SYSTEM NOTE Data Connection Analogue Signal Connector The Varian ELS detector is supplied with a 1V analogue output cable PL0890 0300 that allows data collection via an A D interface such as a Star MIB 800 module The gold end fitting of the analogue cable plugs into the gold pin on the rear of the detector and the opposite end connects to the positive and negative inputs
48. tion of a pump if the unit reports an error condition The Varian ELS detector is also equipped with one contact closure which is normally open and two TTL logic inputs both active low with internal pull up resistors to 5 V Pump stop facility must be employed if the instrument is to be left unattended or if units are stacked O description Pin number Remote Start 14 amp ground Remote Standby 13 8 ground NOTE Remote A Z 7 amp ground Output Pump stop contact closure normally open 3 amp 10 Ground to case 1 5 6 11 Varian ELS detector I O connections The instrument is not supplied with a 15 pin I O cable for the I O socket or remote start cable For remote start operation and I O connections additional cables are required and can be purchased from Varian Inc Remote start cable Part 4 PL0890 0350 I O Cable PL0890 0345 3166 27401 6 Varian ELSD Service Manual Installing the Detector Connect the power cord to the IEC inlet at the rear of the unit e Check the operating voltage of your instrument 110V 2A or 230V 2A on the IEC inlet fuse holder on rear of unit e Attach the gas inlet tube to the gas inlet at the back of the instrument e The gas connection is a 4mm OD push in fitting The nitrogen gas should be dry filtered and have a minimum inlet pressure of 60psi 60 100psi is required to achieve the maximum operating instrument gas flow rate of 3 25 SLM throughout the temperature rang
49. uliser from the mount as shown Check the nebuliser for damage or blockages To install the nebuliser see installing the nebuliser section pg20 DO NOT PLACE THE NEBULISER IN AN ULTRASONIC BATH BLOCKAGES MUST BE REMOVED USING A SPECIAL CLEANING TOOL NOTE 6 27401 19 Varian ELSD Service Manual Installing The Nebuliser When required If Nebuliser becomes blocked Tools required Allen keys Spanners Parts required ELSD Nebuliser 0890 0390 t Ensure that the detector has cooled down before proceeding e Ensure that all gas and solvent connections are disconnected ATTENTION prior to proceeding Check that the nebuliser is not damaged before removing from packaging Fit the gas connector and finger tight connector to the nebuliser as shown Connect the nebuliser to the inlet tubing via the union connection 1166 27401 Varian ELSD Service Manual 20 Very carefully insert the nebuliser into the nebuliser mount Connect the gas tubing to the nebuliser To check the correct gas flow is being delivered to the nebuliser connect a gas flow meter in line between the gas regulator shown and the nebuliser NOTE the instrument needs to be switched on and set to run mode The correct gas flow to the nebuliser should be 0 40SLM If the gas flow is incorrect adjust the gas regulator by pulling down the black knob and twisting until the correct flow is
50. vaporator tube 3166 27401 Varian ELSD Service Manual 34 Replacing The Evaporator Heater Assembly Evaporation Tube When required Faulty Heater Evaporation tube become irreversibly contaminated Tools required Allen keys Spanner Parts required Evaporator Heater Assembly No Cartridge PL0890 0480 Open the detector s side panel using the access key as shown Remove the evaporator tube from the nebuliser chamber as described in Replacing the nebuliser chamber pg 24 Do not discard evaporator cartridge Carefully remove the opposite end of the evaporator tube from the optical block assembly 1566 27401 35 Varian ELSD Service Manual Disconnect the two Evaporator Heater Assembly cables from the main control board EVAP HTR amp EVAP and remove the evaporator heater assembly Insert the New Evaporator Heater Assembly into the optical block Insert the Cartridge into the Evaporator heater assembly and insert assembly into the nebuliser chamber Connect cables EVAP HTR amp EVAP to main control board Ensure the evaporator cartridge is inserted into the evaporator assembly prior to reassembly otherwise the instrument will not perform correctly NOTE 1566 27401 Varian ELSD Service Manual 36 Replacing The Mass Flow Controller When required Faulty Mass Flow controller Tools required Allen Keys Parts re
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