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SERVICE MANUAL ISCUS Flex 8003200

1. Issued June 2010 8003399 Chassie 8003399 Chassie ISCUS Service Manual 80038664 Issued June 2010 3 34 8003399 Chassie ISCUS Service Manual 80038664 Issued June 2010 3 36 ISCUS Service Manual 80038664 8003399 Chassie Issued June 2010 ISCUS Service Manual 80038664 8003399 Chassie Parts List Position CMA P N Description Qty 1 8001934 Chassis Bottom 1 2 8002182 Foot 3 8003859 Spacer M3x8 4 8002661 Inner Chassis 1 5 8002680 Fan Module 1 6 8002254 Printer mounting plate 1 7 8001951 Holder for manual upper 1 8 8001952 Holder for manual lower 1 9 8002605 Mains 100 240 V 1 10 8002405 Cover 1 11 8002402 MRT M4 x 16 A4 4 12 8001474 Washer BRB 4 3x8x0 8 A4 4 13 8002438 MRT M4x6 A4 10 14 8001973 MRT M3x6 A4 9 15 8002300 MFT M3x6 A4 2 16 8002436 MFT M4x8 A4 1 17 8002616 Fastener for cover 4 18 75404025 MRT M4x25 A4 3 19 8001835 MRT M3x8 A4 10 20 8002791 Panel 1 21 8002755 Cover 1 22 8002805 Ground connection wire 1 23 75140430 Washer AZ 4 3 A4 10 24 75140320 Washer AZ 3 2 A4 5 25 8002698 Cable grommet 3 26 8002699 Cable grommet 1 21 8003313 Washer FBB 3 1
2. Print log file and check that the printer works 2 If it does not print check that the paper is installed correctly and flat printer cable is not damaged 3 Ifthe release button on the front lid of the printer is pressed slightly the printer shall feed the paper 4 Replace the printer if it is still not responding Replacement of the Printer 1 Remove the main cover of the ISCUS analyser See 9 1 how to remove the main cover 2 Remove the mounting screws for the Printer The screws can be reached when the printer is opened and the printer paper is removed See picture below Disconnect the cable from the Printer Remove the Printer from the chassis Place the new Printer into the chassis emos re Reassemble in the opposite order Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 10 1 10 Cleaning instructions 10 1 General cleaning instruction 1 Turn off the ISCUS analyser and disconnect the power cord 2 Remove the cover of the ISCUS analyser 3 Brush the dust off from the fan filter Make sure that no dust is inside the ISCUS Flex 4 Clean the following parts with 70 ethanol A Cannula guide B All the sliding surfaces of the XZ module C All the visible surfaces from the outside 10 2 Disinfecting and cleaning of the detector cell and liquid system 1 Activate the service software mode by pressing the Maintenance button and type the following code 1 shift A 2 s
3. actual motor encoder vs Check conveyer belt position and the ex pected motor encoder Check motor encoder position This means and connectors that the motor did not manage to move to the desired position 7 MoveZRela Moves the cannula down until the 2 Z HOME POSITION Check photo interrupter tiveCannula the cannula sensor is activated e g photo interrupter is un and connectors by a vial expectedly activated 4 Timeout Check Z conveyer belt Cannula sensor photo Check cannula sensor interrupter not activated photo interrupter and connectors 5 The cannula sensor is Check for obstructions active before the move Check cannula sensor ment started photo interrupter and connectors Check database settings 10 Timeout Check for obstructions Cannula motor encoder Check motor encoder does not move and connectors 11 Deviation between the Check for obstructions actual motor encoder RR Check conveyer belt position and the ex pected motor encoder Check motor encoder position This means and connectors that the motor did not manage to move to the desired position 10 MoveRela Moves the wash syringe aspi 2 WASH HOME Check photo interrupter tiveWash rate dispense POSITION photo inter and connectors Pump rupter is unexpectedly activated 10 Timeout Check for obstructions Wash syringe motor Check motor encoder encoder does not move and connectors 11 Deviation between the Check for obstr
4. Microdialysis Service Manual ISCUS 8003866A Proposed Actions The table below displays combinations of method numbers and errors and proposed actions if they occur lt E o E Internal Short Description 3 Error Measures e Name S 3t 1 GoTo Moves the cannula to the home 4 Timeout Check for obstructions nomen positio i along me AXIS X home photo inter Check X photo inter left right The photo interrupter i X HOME POSITION defines the rupter not activated rupter and connectors home position 10 Timeout Check for obstructions X motor encoder does Check motor encoder not move and connectors 18 Z home photo inter Check z photo inter rupter is not active X rupter and connectors movement is forbidden so that the cannula is not Check Z conveyer belt broken 21 X home photo inter Check X photo inter rupter not activated after rupter and connectors movement limitation 2 GoTo Moves the cannula to the home 4 Timeout Check Z conveyer belt nomed posman along iie Ze Z home photo inter Check Z photo inter Up downs he photo interrupter rupter not activated rupter and connectors Z HOME POSITION defines the i home position 5 Cannula sensor photo Check for obstructions ON is cho dad Check Z conveyer belt edly active Check Z photo inter rupter and connectors 10 Timeout Check for obstructions Z motor encoder does Check motor encoder not move
5. Wash bin position Press Start again Check that the centre mark of the cannula guide correlates to the centre mark of the wash bin Adjust in X led by clicking right or left of the arrow on the ruler Press Enter to save the new value Press stop and the cannula goes back to the wash bin Production Step 3 9 Adjust Syringes Press Start and wait for the syringes to go to the home positions Unlock the adjustment screw on the sample syringe and adjust it up until the syringe piston hits the top Then back it off half a turn and lock it Unlock the adjustment screw on the wash syringe and adjust it up until the syringe piston hits the top Then back it off one turn and lock it Press Stop Issued June 2010 6 3 ISCUS Service Manual 80038664 10 Adjust the LED light levels Choose which light level you want to check VIS or UV Press Start ISCUS will now measure the signal level It takes approximately one minute If you want to check the level on the other light press Stop and change to UV or VIS When the main cover is removed it is possible to adjust the LED s with the trim potentiometers on the detector module To adjust VIS use the left and UV the right potentiometer Normally the potentiometers are set to maximum If the signal is very high it is recommendable to adjust it down to 3000 mV The ISCUS can measure correct even with signal levels down to 100mV 11
6. 3 Set time Used only in CMA production of the ISCUS Flex Control Panel Makes access to windows control panel Mostly used when the language regional settings and time date is changed 4 Adjust Reagent Analysis Position This parameter is normally set to 0 10 just because the vials can otherwise hit the lid during the mixing The angle can be adjusted by pressing the minus button twice When you are satisfied press Stop Wait until the instrument has calculated the angle Press Apply to confirm 5 Adjust Reagent Mix Position Put in a reagent cassette and press Start Adjust the reagent holder position by clicking the minus button Click several times until the cassette hits the rear stop Then move the cassette five steps with the plus button Then press Stop Wait until the instrument has calculated the angle Press Apply to confirm Press Test to check that the movement is OK Production Step 2 6 Adjust Vial Analysis 7 Adjust Hatch Close 7 Adjust Vial Lock Position Position Position Current 0 0 Start Current 0 0 mm start Current XX es EA E ii ee position Apply position Apply position EEE aH a n En Proposed 0 0 Proposed 0 0 mm i A Please NOTE that setting the general offsets affects the wash 8 Adjust XZ Offset bin position Therefore set the general offsets first General offsets C Wash Bin Position C Glucose Position Reset 6 Adjust Vial Analysis Position
7. 40 1 18 8003387 Bearing 2 19 8003237 Cassette holder compl 1 20 8003314 Washer FBB 4 1 2 21 8003313 Washer FBB 3 1 2 22 8002696 Cable grommet 1 Issued June 2010 3 6 ISCUS Service Manual 80038664 8003222 Z Module Issued June 2010 8003222 Z Module ISCUS Service Manual 80038664 Issued June 2010 8003222 Z Module ISCUS Service Manual 80038664 Issued June 2010 8003222 Z Module ISCUS Service Manual 80038664 Issued June 2010 ISCUS Service Manual 80038664 8003222 Z Module parts list Position CMA P N Description Qty 1 8003221 Carriage complete 1 2 8001852 Slide bearing NW 02 17 special 1 3 8001716 Cannula guide 1 4 8002390 Screw MFT 3x8 A4 1 5 8001717 Cannula holder compl 1 6 8001855 Z circuit board complete 1 7 8002404 Spring 1 8 75403010 Screw MRT M3x10 1 9 8001895 CAB ISCUS Flat Z 1 10 8001769 Z cable Locker for tooth belt 1 11 8002302 Screw MRT 3x6 A4 1 12 M000322 Pin CP h8 2x16 A2 1 13 8001835 Screw MRT M3x8 1 14 8001821 Spacer DRN 3160 x4 1 15 8003227 PCA XZ CB compl 1 16 75140320 Washer AZ 3 2 2 17 8003313 Washer FBB 3 1 1 18 8002490 P Molex 6471 1x3 1 19 5870030 P HY Crimpterminal 3 20 8002461 CAB ISCUS Encoder Z 1 Issued June 2010 3 11 8002650 Syringe pump Module ISCUS Serv
8. Find the center volume for the machine Press Find Center volume and the ISCUS will start looking for the center volume It takes about 3 minutes if eve rything works OK If it takes longer it may depend on air in the liquid system Check cannula connection and peek tubing connections If the check box is marked then it will validate the new center volume 12 Adjust Load Positions Mark Reagent and press Start The front lid will open Adjust the Hatch with the plus minus button The open ing arm shall be 5 mm from hitting the cover on the right side of the lid Adjust the opening of the lid by the plus or minus button Reagent vial to make an easy access to the EM Then press Stop and then Apply Do the same procedure with the Vial parameter Adjust the opening of the lid by the plus or minus button Reagent vial to make an easy access to the vials Production Step 4 Optical Repeatability 0 5 yl Repeatability 0 2 pl Issued June 2010 6 4 ISCUS Service Manual 80038664 6 5 13 Run a production test Insert production cassette To run a production test Load the production cassette by pressing Reagents and then Change and choose Pro duction Put in the production cassette which contains Amido black solution NaNO H 0 Glucose reagent and calibrator Close the reagent lid by pressing Return Choose which of the tests you want to run Optical test 2 usua
9. P N Description Qty 1 8003150 Rinse bottle housing 1 2 8002185 Holder for the bottle sensors compl 1 3 8001848 Slide bearing 2 4 8002188 Handle 1 5 8001965 Rail for slide bearings 1 6 8003156 Waste tube complete 1 7 8003161 Rinse tube complete 1 8 8003152 PBA ISCUS Flex sensor compl 1 9 8002199 Flag Screw 1 10 8003154 Guide left 1 11 8003155 Guide right 1 12 8001813 Screw MRT 3x8 A4 6 13 8002401 Washer BRB 6 4x12x1 6 A4 2 14 8001475 Screw MRT 4x12 A2 2 15 8001929 Screw MRT 3x4 A2 2 16 8002345 Sticker Rinse 1 17 8002346 Sticker Waste 1 18 8001831 Screw MRT 4x8 A4 1 19 75200400 Nut M6M M4 A4 2 20 75140430 Washer AZ 4 3 A4 3 21 8002449 Spacer 1 22 75063006 Screw SK6SS 3x6 A4 1 23 8003859 Spacer M3x 8 A2 1 24 75200300 Nut M6M M4 5 25 75140320 Washer AZ 3 2 A4 5 26 8002800 No longer in use 1 27 8002522 Screw LKCS M4x8 1 28 8003151 Waste flag arm complete 1 29 8003146 Magnetic lock 1 30 8002390 Screw MFT M3x8 4 31 8003313 Washer FBB 3 1 7 32 8002404 Spring 0 4x4x15 1 33 75003008 Screw MCS M3x8 4 34 M000309 Soldering eye M3 1 35 75210300 Nut ML6M M3 1 36 8003313 Washer FBB 3 1 1 37 8002544 Cable clip ID 9 2 Issued June 2010 3 30 ISCUS Service Manual 80038664 8003399 Chassie Issued June 2010 3 31 ISCUS Service Manual 80038664 8003399 Chassie Issued June 2010 ISCUS Service Manual 80038664 8003399 Chassie Dubbelh ftande tej
10. Printer lid Thermal Print Paper ISCUS package of four Issued June 2010 4 1 5 ISCUS Service Manual 80038664 Maintenance service Recommended interval Once per year Maintenance consumables 1 x Maintenance service kit ISCUS 8002792 The Maintenance kit includes D000011 Gasket syringe to syringe adapter 2 pcs 8001721 Sample Cannula ISCUS 1 pcs 7431070 Sample Syringe 500 ul 1 pes 7431071 Wash Syringe 1 ml pes Maintenance service Que pa de 5 10 11 12 Remove the cover and clean ISCUS Flex See cleaning instruction chapter 10 Start the service software by pressing the Maintenance button in the cog wheel menu and then type the fol lowing code 1 shift A 2 shift B and 3 Click OK and wait for the system to restart Open the Service tab and replace the cannula See replacement of cannula chapter 9 2 Open the Service tab and replace both syringes See replacement of syringes chapter 9 3 Open Step 3 tab Check the signal levels by pressing Start If the levels are lower than 1000 mV adjust the light diodes normally they are adjusted to the maximum level adjust the trim potentiometer for VIS light and do the same on the trim potentiometer for UV light If it is not possible to get the signal levels higher that 1000 mV perform a cleaning of the cell see chapter 10 If it does not help replace to a new cell If major hardware repairs have been done perform the mechanical te
11. Put in a vial cassette and load the first and the last position with a vial and press Start Lift up the cannula and move the X carriage needle by hand to a position above one of the vials Check that the cannula is above the center of the vial in Y led if not adjust with plus or minus button and then check the same with the other vial If the value is not the same for both vials set the value to something in between Press Stop Wait until the instrument has calculated the angle Press Apply to confirm Issued June 2010 6 2 ISCUS Service Manual 80038664 7 Adjust Hatch Close Position Press Start Press the plus button several times until the front lid starts to open Then press five times on the mi nus button Then press Stop Wait until the instrument has calculated the angle Press Apply to confirm 8 Adjust XZ Offset Start to adjust the General offset Make sure that you have a vial in the first position of the cassette Press Start Check that the cannula penetrates the centre of the vial in X led Adjust by clicking left or right of the arrow on the ruler Press Enter to save the new value Then check the Z value Press Start and check that you can see at least 0 5 mm of the tip of the cannula when it penetrates the septa If not adjust with the plus or minus button for Z Press Enter to save Press stop and the cannula will go back to the wash bin Adjust the
12. Rete e ene aoe 3 37 002630 LCD MONILOR E cete eren AAEE EE E AET 3 38 8002630 LCD MONITOR MODULE PARTS LIST ee nnne rtr rtr r rtr r r r rer rererere np p eee 3 43 8003809 ISCUS FLEX ANALYZER eee e e e n I n n n nnn nnn nnn r nnn n rtr n nn n r r a a r r r E r r r eee eee nenea eee eee 3 44 8003809 ISCUS FLEX ANALYZER PARTS LIST eee eee e n n n n n n n rtr rtr rtr rtr rtr r rtr r n nenea eee eee 3 59 4 SPARE PARTS oT 4 1 5 MAINTENANCE SERVICE DE 5 1 G SERVICE SOFTWARE MODE eos etes ene de ia eo Pe ei ro EE P PEE RE gebe FE So EUER o see E SERE OE PR OT OR Ur poe OP POR aee sense 6 1 Ts URONDINRORIS OTOJUL O 7 1 7 1 HARDWARE FAULTS eec a nal EE e cutia Bites tek ER ee ete e catia Sates i ad tut cad 7 1 7 2 CALIBRATION CONTROL SAMPLES OR NORMAL SAMPLES FAULTS suisses 7 2 8 HOW TO DECODE THE ISCUS LOG FTILE 4 eee ee ee eee erento oeste tne e etta eee seen conoce eee eaae s ee enne eee en eese en aa 8 4 THE oT 8 4 OTHER ERRORS a 8 5 Ar B S HDI 8 6 9 CHECK REPLACEMENT OF PARTS AND MODULES ecce eee ee ee eene eee tone eee tn eese ea ee ee tn ases teen eese en aa 9 1 9 1 HOW TO REMOVE THE MAIN COVER ess eee s stesse 9 1 9 2 CHECK REPLACEMENT OF CANNULA ccccccscsescsecescsescsesvevevevsvevsvsvevevsvsvevevsvsvsvsvsvevsvevevevevevsvevevevevevevevevevevevevevens 9 6 9 3 CHECK REPLACEMENT OF SYRINGES
13. cables and Peek tubings Remove the red lift handle Remove the two screws for the two slide bearings and lift out the Rinse Waste level sensors See picture below Disconnect the cables and Peek tubings from the Rinse Waste level system Remove the Rinse Waste level sensors from the chassis Reassemble in the opposite order Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 9 13 Check Replacement of Display module Check of the display module 1 Turn on the ISCUS 2 The display shall light up after 15 to 30 seconds 3 Ifthe display has not lightened up check that the dimmer wheel on left side of the ISCUS is not set in the darkest mode Check all cable connections to the screen Check that the Smart media card is mounted on the main board Replace the display module if nothing of the above mentioned helped Sas The problem can also depend on a faulty main board Replacement of the display module 1 Remove the main cover of the ISCUS analyser Disconnect the cables to and from the Display module Remove the mounting screws for the Display module Remove the Display module from the chassis Place the new Display module into the chassis Mount and secure the mounting screws for the new Display module Connect the cables to and from the new Display module oN Aw Boo Mount the main cover of the ISCUS analyser 9 14 Check Replacement of Printer Check of the Printer 1 Press
14. on idle To reset the motors just press Reset all or close the window Itis also possible to run a Loop or macro for the X Z rinse and pipett syringe Ifthe Display values button is choosen the active sensors will be displayed Issued June 2010 ISCUS Service Manual 80038664 Module Test tab XZR Syringes Cell Test Chassis Cover Insert empty bottles in all Iterations Insert a production rack before positions and XX empty vials starting using a code The result before starting and work queue will be displayed E Detailed on the Step 4 tab Stat Os ay Start Chassis Cover This function checks the sensor switches for the Rinse and Waste bottle Follow the instructions on the screen When you have performed the test it is possible to print out a report to save the result S Rinse Waste handle UP D inactive bSRinse Waste handle DOWN 0 inactive 2 Rinse bottle present Waiting 1 ACTIVE Rinse bottle NOT present Waiting 1 ACTIVE Waste bottle present Waiting 1 ACTIVE Waste bottle NOT present Waiting 1 ACTIVE Rinse bottle empty Waiting 0 inactive Rinse bottle NOT empty Waiting D inactive Q waste bottle full Waiting 0 inactive Q Waste bottle NOT full Waiting D inactive ze E ES Issued June 2010 6 9 ISCUS Service Manual 80038664 6 10 XZR Open the Production Step 4 and press Reagent and Change and mark Production Insert an empty reagent cassette with ca
15. 002602 8001877 J3 d Power Green en included 29 Green led in cable Power Power fail Is not in use Is notin use yet Ground cables CNE si ou M EN let Rinse Waste 8002800 Rinse Waste XZ module module module Syringe pump 8002800 Syringe pump Chassis module EN at the bot Power supply 8002800 Power supply Syringe pump module at the top Ground connec 8002805 Chassis Power supply tion wire Issued June 2010 12 3 CMA Microdialysis Service Manual ISCUS 8003866A 13 1 13 Control of ISCUS Flex Analyser To check the performance of the ISCUS Flex it is recommendable to put in a control sample bottle You can do it in two different ways 1 Put in a Control sample bottle in the reagent holder at the same time you change reagents or prepare a vial with control liquid and run it as a normal sample for the patient 2 Check Control samples information for correct levels The information sheet is enclosed with each con trol sample package Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 14 1 14 Safety e National standards and local directions must be fulfilled at installation and service e Follow the packaging un packaging procedure to avoid personal injuries equipment damage or prop erty damage e Never use the device near mobile telephones CB radios or other forms of radio communication and or electromagnetic fields These may affect the performance of t
16. 003313 Washer FBB 3 1 A4 19 21 75220300 Lock Nut M3 1 22 8002758 Cable ISCUS Backlight Pot 1 23 8002781 Cable ICUS LCD 1 Issued June 2010 3 44 ISCUS Service Manual 80038664 8003809 ISCUS Flex Analyzer i N m m k OD Es l dD y Issued June 2010 8003809 ISCUS Flex Analyzer ISCUS Service Manual 80038664 Issued June 2010 3 45 3 46 ISCUS Service Manual 80038664 8003809 ISCUS Flex Analyzer 9 amp cp YG 9 MEN N X SEED LR am ES 11 Po N FI N B N b N Issued June 2010 8003809 ISCUS Flex Analyzer ISCUS Service Manual 80038664 Issued June 2010 3 47 ISCUS Service Manual 80038664 8003809 ISCUS Flex Analyzer DETAIL D SCALE 1 1 Issued June 2010 8003809 ISCUS Flex Analyzer 8003809 ISCUS Flex Analyzer 3 51 ISCUS Service Manual 80038664 8003809 ISCUS Flex Analyzer Issued June 2010 3 52 ISCUS Service Manual 80038664 8003809 ISCUS Flex Analyzer Issued June 2010 3 53 ISCUS Service Manual 80038664 Issued June 2010 8003809 ISCUS Flex Analyzer TS j XJ 8003809 ISC
17. 4 222 nain a ii ceca ue eee dieta ce 9 7 9 4 CHECK REPLACEMENT OF SYRINGE PUMPS MODULES eee 9 8 9 5 CHECK REPLACEMENT OF THE SOLENOID VALVE ee eee eee eene en esses essses esse sess ss sss esse sess esses esee eee 9 8 9 6 CHECK REPLACEMENT OF DETECTOR CELL eee 9 9 9 7 CHECK REPLACEMENT OF DETECTOR MODULE aaa 9 10 9 8 CHECK REPLACEMENT OF XZ MODULE ae sees eee 9 11 9 9 CHECK REPLACEMENT OF MAIN BOARD eee eee 9 11 9 10 CHECK REPLACEMENT OF POWER SUPPLY ssssseseesessseveseveseveceveserevereveverereveseseseseseceseseveceseseseseresesesereeenens 9 12 OIL REPLACEMENT OF FAN 2 ceea a 9 13 9 12 CHECK REPLACEMENT OF RINSE WASTE MODULE scesseeseseseseseveveseceseveseseseseseceseseseceseseseseseseseseresenens 9 13 9 13 CHECK REPLACEMENT OF DISPLAY MODULE nene eee nene eee eee eee n enne enne ene n ene enne enne eerte eene nenne enne 9 14 9 14 CHECK REPLACEMENT OF PRINTER cceeeeeeeeeeeee eene nenea nene nene eee denen ene eere ene EEEE EEEE EEEE eet 9 14 10 CLEANING INSTRUCTIONS 2 eos soe oo oo eaae erar aee eH pose t Pn aa aee rea o E PE ES o NEL EE a eee Eo soia VERE e Verna Ree Dc cra inn Pase be sense pa ses 10 1 10 1 GENERAL CLEANING INSTRUCTIONS eu essen il ere p aa ee het pasta ete d ire edes ere psi 10 1 10 2 DISINFECTING AND CLEANING OF THE LIQUID SYSTEM na aaa entente entente innen 10 1 11 CIRUIT BOARD LIST 11 1 12 CABLE e he OL Bs O oC 12 2 13 C
18. 403005 Screw MRT 3x5 A4 33 004 8002502 Spacer screw DSS M3050 x 18 10 005 8002190 Detector module ISCUS 1 006 8002642 Detector mounting plate 1 007 8001819 Screw MRT 3 x 12 A4 3 008 8002412 Detector cell ISCUS complete 1 009 8002630 LCD module complete 1 010 8002665 Adjusting screw 1 011 8002421 Adjusting screw 1 012 8002793 Screw P6SS M4 x 30 A2 2 013 8003351 Bottle lid complete ISCUS Flex 1 014 75023005 Screw LKCS 3 x 5 A4 3 015 8001932 Handle ISCUS 1 016 8002453 Screw MRT 4 x 20 A2 2 017 8002676 Rear cover 1 018 8001962 Screw MRT 5 x 10 4 019 8003197 Front cover complete ISCUS Flex 1 020 8001930 Adjustment knob 1 022 75063006 Screw SK6SS 3x 6 A4 1 023 8001813 Screw MRT 3 x 8 11 024 8002650 Syringe pump module complete 1 025 8003203 Rinse and waste bottles module compl ISCUS Flex 1 026 8002444 Screw MRT 4 x 5 A4 4 027 8003180 XZ module complete ISCUS Flex 1 028 8001948 Printer ISCUS 1 029 8002658 Printer lid 1 030 M000062 Washer BRB 3 2 x 12 x 1 fzb 5 031 75140320 Washer AZ 3 2 A4 lock 14 032 8002664 Air shield 1 033 8002768 Spacer tube 3 2 x 5 L 3 2 4 034 8002797 Runoff sheet 1 Issued June 2010 ISCUS Service Manual 80038664 035 8003332 Overlay ISCUS Flex 1 036 8002481 Seal 17 x 5 mm 0 7m 037 8003313 Washer FBB 3 1 15 038 8001831 Screw MRT M4 6 039 75140430 Washer AZ 4 3 6 040 M000105 Washer BRB 3 2x7x0 5 2 041 8003363 Holder for the front lid 2 042 8003347 Spac
19. A4 14 28 8003647 Spacer 2 29 8001479 MRT M3x16 2 30 8003410 Spring 4 31 8002617 MTF M4x16 A4 1 32 8002674 PCA ISCUS Pot CB 1 Issued June 2010 8002630 LCD Monitor ISCUS Service Manual 80038664 L 25 bredd 15 Issued June 2010 3 38 8002630 LCD Monitor ISCUS Service Manual 80038664 Issued June 2010 3 39 8002630 LCD Monitor ISCUS Service Manual 80038664 Issued June 2010 3 40 8002630 LCD Monitor ISCUS Service Manual 80038664 Issued June 2010 3 41 8002630 LCD Monitor ISCUS Service Manual 80038664 Issued June 2010 ISCUS Service Manual 80038664 8002630 LCD Monitor Module Parts List Position CMA P N Description Qty 1 8002633 LCD Chassis complete 1 2 8002230 LCD Display with Touch screen 1 3 8002603 PCB Display adapter New 1 4 8002637 Shield plate 1 2 8002338 PCB ISCUS Backlight unit 1 6 8002636 Cover 1 7 8002638 Mounting plate right 1 8 8002639 Mounting plate left Nut 1 9 8002443 Spacer screw M3055 x 10 2 10 8002916 Spacer screw M3050 x 8 4 11 8001973 Screw MRT M3 x 6 A4 15 12 75200300 Nut M6M M3 A4 2 13 8002784 Contact finger 1 14 8002782 Contact finger 1 15 8002801 EMC protection 3 16 8002647 Display support 2 17 8001479 Screw MRT 3 x 16 A4 1 18 7430522 Tape double adhesive 1 19 75210300 Nut ML6M M3 A4 4 20 8
20. CUS 8003866A 14 2 Waste disposal AN Biological risk e Rinsing and waste fluid can be disposed as water unless there is a risk for infection NOTE The Waste fluid may be contaminated with e g hepatitis Be sure to use normal hospital routines If hospital requirements do not permit you to reuse the waste bottle replace it with a new empty bottle using the cap of the new bottle to seal the old bottle e Reagents and calibrator can be disposed as normal waste e Microvials can be disposed as normal waste unless there is a risk of infection NOTE The samples may be contaminated with e g hepatitis Be sure to use normal hospital rou tines e Paper rolls can be disposed as normal waste e The sample cannula shall be disposed according to hospital routines for needles e For disposal of the Analyzer or parts of it contact your CMA representative Transportation and Packaging e For transportation outside the hospital use the original interior and exterior packaging e The package shall be transported upright and carefully NOTE Before transporting ISCUS you need to follow the shutdown procedure The shutdown procedure activates a transport security lock that prevents the sample cannula from moving and getting damaged By emptying the wash waste bottles and removing the reagent cassette you also reduce the risk of equipment damage which is not covered by any service or warranty agreement Issued June 2010
21. ISCUS Service Manual 80038664 SERVICE MANUAL ISCUS Flex 8003200 Contents 1 DESCRIPTION p E A 1 2 PUER QUETMUEN iile 05 2 1 3 ASSEMBLY DRAWINGS P 3 1 8003180 XZ MODULE tito 3 1 8003180 X7 MODUEEB PARTS LIST cioaca cade bets de accea tdi ast 3 6 8003222 7 MODULE 5 tiet eee da teretes iudaic uta cau tt de ti atu iati cet rite t de etalat oua aaa ati do ast 3 7 8003222 7 MODULDEPARTS LIST 4 eoi co Han ti ia a dada da 3 11 8002650 S YRINGE PUMP MODULE eee nn nee ce retener eee eene ceai de note inainta indata cette 3 12 8002650 SYRINGE PUMP MODULE PARTS LIST unes nen n nnn nnn nn nnn r rtr rte r r r e n r r n e r n n p n nne eee eee 3 21 8002412 DETECTOR CELL een Bica ga atata sueta isti mense Re 3 22 8002412 DETECTOR CELE PARTS LIST ette ERR s ta tatea ene RM 3 23 8002190 DETECTOR MODULE 2 25 AA AA 3 24 8002190 DETECTOR MODULE PARTS LIST eee eee eee eee eee eee eee aaa 3 25 8003203 RINSE 7 WASTE MODULE teinte Rte ete EE atata er uda 3 26 8003203 RINSE WASTE LEVEL SENSORS ccccccccconcnononnnnnnononononononononononononononononnnnnnnonononnnonononononononononononononononanananonananona 3 27 8001970 RINSE WASTE MODULE PARTS LIST eee eee n n eee rtr r r r rte r r r e r r rer rrr rererere ereet 3 29 8003399 CHASSIE Krane A A A E E IN 3 30 3003399 CHASSIE PARTS LIST uet
22. ONTROL OF ISCUS ANALYSER suis eae ete ea essen dee see sev entered ES AAA RT 14 1 Issued June 2010 1 1 ISCUS Service Manual 80038664 1 2 1 Description ISCUS Flex is a selective chemistry analyser designed for the small sample volumes obtained when sampling with Mi crodialysis Sample volumes are 0 2 2 0 uL per test reagent volumes 5 0 14 8 uL per test depending if it is clinic or pre clinic This product is CE marked in conformity with the requirements of the Invitro Diagnostic Device Directive 98 79 EEC Use only CMA Microdialysis spare parts accessories and consumables CMA authorised personnel shall perform all service ISCUS Flex shall be serviced once every 12 months by a qualified service engineer certified by CMA Read the SAFETY chapter before servicing the ISCUS analyser The movements of the microprocessor controlled sample and reagent cannula is based on a XZ co ordinate principle while the sample and reagent tray moves in the Y co ordinate This allows the system to locate samples reagents and calibrator X is left to right Z is up and down Samples are collected in specially designed sealed microvials that are placed in a microvial holder The microvial holder has 3 positions for direct analysis and 1 position for the analysis of control samples The sample cannula pierces the upper stopper of the microvial to aspirate a sample The sampling arm is equipped with a cannula guide that also secures t
23. SNOSI Issued June 2010 3 Assembly drawings 8003180 XZ Module ISCUS Service Manual 80038664 Issued June 2010 3 1 8003180 XZ Module ISCUS Service Manual 80038664 Issued June 2010 3 2 ISCUS Service Manual 80038664 8003180 XZ Module Qt Es n x gt un A Issued June 2010 8003180 XZ Module A wi No e wu m iN A A ih Ar C 1 N A SS i Jo D ISCUS Service Manual 8003866A AL L e AS ESA O Issued June 2010 3 4 8003180 XZ Module ISCUS Service Manual 80038664 Issued June 2010 3 5 ISCUS Service Manual 80038664 8003180 XZ Module parts list Position CMA P N Description Qty 1 8003181 Chassis complete 1 2 8003173 Shaft 1 3 8003194 X rail 1 4 8003175 PCA ISCUS Flex connection board 1 5 8001705 PCA ISCUS Flex X home sensor 1 6 8003188 Shaft Rubber stopper sensor 1 7 8001837 Screw MRT M4x5 2 8 8002302 Screw MRT M3x6 2 9 75130400 Locking clip RS 4 6 10 8002619 Support arm 1 12 8001831 Screw MRT M4x8 2 13 8002692 Insulating sheet 1 14 8003307 Tooth belt TN15 2 15 8003222 Z module ISCUS Flex 1 16 8003240 Lid Opener compl 1 17 8003232 Tooth wheel TN15
24. US Flex Analyzer ISCUS Service Manual 80038664 Issued June 2010 8003809 ISCUS Flex Analyzer ISCUS Service Manual 80038664 Issued June 2010 ISCUS Service Manual 80038664 3 56 8003809 ISCUS Flex Analyzer Issued June 2010 3 57 ISCUS Service Manual 80038664 8003809 ISCUS Flex Analyzer Issued June 2010 8003809 ISCUS Flex Analyzer ISCUS Service Manual 80038664 Issued June 2010 ISCUS Service Manual 80038664 8003809 ISCUS Flex Analyzer Parts List Position CMA P N Description Qty 001 8003399 Chassis complete ISCUS Flex 1 002 8003323 Main Board ISCUS 4 1 003 75
25. and connectors 21 Z Home photo inter Check for obstructions rupter is not active when Chez conveyer belt the movement is com pleted Check Z photo inter rupter and connectors 3 GoTo Moves the pipetting syringe to 4 Timeout Check syringe conveyer HomeSyr the home position fecu belt Pipetting syringe home The photo interrupter SYRINGE photo interrupter not Check syringe photo HOME POSITION defines the activated interrupter and connec home position tors 10 Timeout Check for obstructions Pipetting syringe motor Check motor encoder encoder does not move and connectors 21 Pipetting syringe home Check syringe conveyer photo interrupter not belt activated after the Check sige photo movement was com interrupter and connec pleted ios Issued June 2010 8 6 CMA Microdialysis Service Manual ISCUS 8003866A Internal E Short Description Error Measures e Name S FA 4 MoveRela Moves the cannula left right 1 Parameters out of range Check settings in data tiveX base 2 X HOME POSITION Check X photo inter photo interrupter is un rupter and connectors expectedly activated 10 Timeout Check for obstructions X motor encoder does Check motor encoder not move and connectors 11 Deviation between the Check for obstructions petal motet COURT Check conveyer belt position and the ex pected motor encoder Check m
26. ange and mark Production Insert an empty reagent cassette with caps and 16 empty vials Press Open Close Reagent and put in an empty or old reagent cassette Use the production cassette mode Go back to the Service tab and press Go to all positions The cannula will now go to all positions once If the button is pressed several times it will perform several cycles Remove all patient data Removes all data from the database Export Database to file Will export the database to a text file on a SD card or USB memory Manual Control f i H i i i i i i i i Open RINSEto CANNULA Rinse pump io e NN 4 1 1 H H H 1 1 1 H 1 1 1 1 H H 1 H 1 1 1 H 1 H H H H 1 H 1 H H H H 1 H H H H 1 1 1 1 1 1 H 1 1 H 1 H 1 1 H H H 1 1 H 1 H 1 1 1 1 1 1 a Set motor IDLE Loop Count both X and Y Step size 1 10pL EEE EEE EEE EEE EEE iy H i i 1 i i 1 i i i i i i H i i 1 i i H i i H i i 1 i i 1 i i H i i H i i H i i i i i 1 i i i i i H i i H i i i i i i i i i i i i i H i i H i i H i i H i L If you want to move the different movements manually it is possible to set the specific motor on idle Just choose a button or buttons of which motor you want to set on idle Then the motor for that specific movement will be set
27. cables for the new syringe pump module Mount the Peek tubings Rinse fluid bottle valve solenoid and detector sample syringe NES M SON E E 2 Mount the rear cover of the ISCUS 9 5 Check Replacement of the Solenoid Valve Check of the Solenoid Valve 1 Enter the service software mode and enter the Service tab 2 Press Wash x 10 to check that no air is visible in the wash syringe after ten cycles 3 Ifthere are large air bubbles in the syringe even after ten flushes check that the syringe is tightened correctly and no cracks are visible in the glass body 4 If there are still large amount of air in the syringe check the connections to the solenoid valve 5 Ifthe connections are tightened and still lots of air in the syringe replace the valve Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A Replacement of the Solenoid Valve 1 Qu ge ces SD Remove the rear cover of the ISCUS analyser Remove the Peek tubing connections on the valve Remove the mounting screws for the valve Remove the valve from the syringe pump module Place the new valve on to the pump module Reassemble in the opposite order 9 6 Check Replacement of Detector cell Check of the detector cell Open the service software mode Type in the password main and enter Step 3 Check the signal levels for VIS and UV by choosing VIS or UV in the menu 10 Adjust the LED light levels and Press Star
28. crew that holds the rear cover 4 Open the front lid Issued June 2010 9 2 CMA Microdialysis Service Manual ISCUS 8003866A 5 Remove the end of the spring from the hinge 6 Unscrew the T 10 screw until it unhooks from the left hinge 7 Remove the right T 10 screw Make sure that the small bushing in the hinge not falls off Issued June 2010 9 3 CMA Microdialysis Service Manual ISCUS 8003866A 8 Remove the front lid 9 Unscrew the T 20 screw on the right side of the bottom Don t remove the screw completely just re lease it five turns Issued June 2010 9 4 CMA Microdialysis Service Manual ISCUS 8003866A 10 Unscrew the T 20 screw on the rear left side of the bottom Don t remove the screw just release it five turns lt 5 11 Do the same with the front right screw Issued June 2010 9 5 CMA Microdialysis Service Manual ISCUS 8003866A 9 6 13 Remove the front cover from the ISCUS Flex 14 When reassembling make sure that the monitor is positioned correctly As far back as possible and the adjustment screws in the rails Start to tighten the lower right screw the two top screws and then the lower left screws 9 2 Check Replacement of Cannula In User software mode 1 2 Go to the cogwheel menu Press Maintenance and then the Sample cannula button Press the Open button The front lid will ope
29. e time is allowed to execute 26 The MCUConfiguration file contains error Read the ISCUS_LOG log for more information 27 The sample rate for the analog input devices is longer then the Timeslot value that is used to cal culate the average value 28 A motor cannot fulfill a movement since Abort has been called 29 REAGENT STEP MOTOR is active 30 VIAL STEP MOTOR is active 1001 TELECA Move Relative bug Issued June 2010 8 4 CMA Microdialysis Service Manual ISCUS 8003866A Other errors There are also some error entries on the form YYMMDD HH MM Error where Error is described in the fol lowing table Error Description XHome n where n is a number Error during x movement XRel n where n is a number Error during x movement ZHome n where n is a number Error during z movement ZRel n where n is a number Error during z movement ZSens n where n is a number Error during z movement WashAsp n where n is a number Error during wash syringe movement WashHome n where n is a number Error during wash syringe movement WashDis n where n is a number Error during wash syringe movement SyrHome n where n is a number Error during pipetting syringe movement SyrAsp n where n is a number Error during pipetting syringe movement SyrDis n where n is a number Error during pipetting syringe movement Issued June 2010 CMA
30. e water levels are below 1000 mV increase the intensity of the LED diodes by turning the potentiometers on the detector unit Left VIS and Right UV 3 Ifitis not possible to get any higher signal level than 1000mV by increasing the tension of the diodes it could depend on a dirty detector cell broken detector cell faulty pre amplifier or broken diode CB Before doing anything else with the trim pots make sure that the fault is not caused by any of the above men tioned See also chapter 10 Cleaning instruction Replacement of the detector 1 See replacement of detector cell 2 Remove all screws from the radiation shield which are above the XZ module 3 Bend out the radiation shield to make access to the three mounting screw on the backside 4 Disconnect the cables from the main board Remove the detector Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 5 Connect the cables for the new detector module Photo sensor board con8 on main board J3 on detectormod ule con49 on main board and the flat cable from diode board on detector module to con 5 on the main board 6 Reassemble as Replacement of Detector cell 9 8 Check Replacement of XZ module Check of the XZ module 1 With the ISCUS shut down and the power off check that the cable connections for the motors are connected properly on the main board 3 Check manually that Reagent holder X and Z movements can be moved freely without any
31. easures e Name S a 11 Deviation between the Check for obstructions actual motor encoder i Check conveyer belt position and the ex pected motor encoder Check motor encoder position This means and connectors that the motor did not manage to move to the desired position 57 MoveRela The command would Report to CMA tiveVial result in a position out P Check definition of po side the valid area n R sitions in the database 2 VIAL HOME Check for obstructions POSITION photo inter A P Check conveyer belt rupter is activated dur ing movement Check photo interrupter and connectors 7 The vial rack hit the bar when going inwards 10 Timeout Check motor encoder A and connectors Vial rack motor encoder does not move 11 Deviation between the Check for obstructions actual motor encoder position and the ex pected motor encoder position This means that the motor did not manage to move to the desired position Check conveyer belt Check motor encoder and connectors Issued June 2010 8 12 CMA Microdialysis Service Manual ISCUS 8003866A 9 1 9 Check Replacement of parts and modules 9 1 How to remove the main cover Tools Below are the required tools for removing the covers 1 Remove the two T 20 screws for the handle Remove the handle Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 2 Remove the T 20 bottom s
32. er DRM 3240x4 1 043 8003353 Front lid Painted 1 044 M000059 Screw CSS ST2 2x6 5 1 045 8003737 Spring 1 046 8002427 Sticker 1 047 8003205 Vial holder complete 1 Issued June 2010 ISCUS Service Manual 80038664 4 Spare parts P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N P N 8001721 7431070 7431071 8002650 8001894 8002412 8002190 8003180 8003323 8002752 8002754 Sample Cannula ISCUS Sample Syringe 500ul Wash Syringe 1 ml Syringe Pump Module ISCUS Fan ISCUS Detector Cell ISCUS Detector Module ISCUS XZ module complete ISCUS Flex Main board ISCUS 4 Solenoid Valve ISCUS Finger tight fitting for the solenoid valve M000032 Nut KEL F M000033 Ferrule KEL F 8001877 8003203 8001904 8001871 8001833 8002765 8002454 8001915 8001948 8002792 8002605 8002630 8003351 8002676 8002802 8001932 8003197 8003353 8002659 8002162 Power supply ISCUS 125W Rinse Waste module ISCUS Flex Stepper motor X Z vial reagent and syringes K ISCUS Photodiode K ISCUS LED K ISCUS LED complete with filters Printer cable Return Pump SP 500 Printer ISCUS Maintenance kit ISCUS Main Power Inlet LCD module ISCUS Rinse waste bottle lid complete ISCUS Flex Rear cover ISCUS Flex Inspection Cannula lid Handle Front cover complete ISCUS Flex Front vial reagent lid ISCUS Flex
33. he device The analyser conforms to EN 60601 1 2 IEC 61326 and shall not be exposed to higher levels of disturbance e Regarding emitting electronic fields ISCUS fulfils Class B according to EN 60601 1 2 IEC 61326 if Ethernet and RS 232 cables are not longer than 3 m e Do not immerse the device in water or any other liquid See the cleaning instruction for specific details e The power cord also serves even when the main power switch is switched off i e disconnect power cord to set power completely off e Disconnect the power cord before cleaning or servicing e Do not connect the power cord to anything else than the appropriate input of the device e The device is only to be used with medical equipment complying with EN 60601 1 EN 60601 1 2 and IEC 60950 Computer equipment e If other equipment is connected to ISCUS in a patient environment the installation must fulfil leakage current and electrical separation requirements according to EN60601 1 1 e g by using a separation de vice The RS 232 serial port and Ethernet port are galvanically isolated from the internal secondary cir cuits of ISCUS with basic insulation according to the requirements of IEC 60950 withstanding 1500 V The USB port is not galvanically isolated from the internal circuits within ISCUS The USB port is intended for service purposes It shall only be used as specified by CMA Microdialysis AB e The device is not intended for use with flammable anaesthet
34. he sample vial in position when sample is aspirated There is a reagent cassette holder for the reagent cassettes The sample reagent cannula pierces a membrane in the cap of the reagent and calibrator bottles to aspirate reagent or calibrator Reagent and sample volumes are controlled by a precision glass syringe of 500 uL A second syringe of 1000 uL is used to rinse the cell and sample reagent cannula with rinsing fluid A solenoid valve is used to select between washing of the system and filling with rinsing fluid Absorbance measurements are made with a single beam filter photometer using a horizontal placement of a capillary flow cell relative to the light path Two different Class 1 LEDs with the wavelengths of 530 nm and 375 nm choose the wavelength respectively Issued June 2010 2 1 ISCUS Service Manual 80038664 2 Block diagram o npour IIST AA U 07 008 a1noq ISEM Jamod sure yy 097008 your MASTI SANOSI SNSI ue SNOSI 9 n pour duind o3uti amp g 0S97008 Jury aBuLI s TOOS USA aus ILOTEYL a durs OLOTETL SNOSI APA prouajos TSLTOOS 00 dS dund umy Od 10u09 um ETEEOOS SNOSI 9mpout 497 0 97008 SAOSI IUT 8761008 SNOST 9 19 duroo anpou ZX 081 008 juoSeow T061008 IPJO PIA 1555552415122 o a a y SMOSI 9 npour 1019319 Japjo 0617008 uq USA pou quae oy SNOSI 1129 1019919 TIPTOOS E S161008 E so npour pu uonounjg orseq XIII
35. hift B and 3 Open the Step 4 tab Open the reagent lid by pressing Reagent Load the ISCUS analyser with a cleaning cassette See Cleaning Cassette below how it can be made Use cassette code and choose the Cleaning option on the screen a Rw D Press OK and the cleaning procedure will start Cleaning Cassette Required items e One empty reagent cassette e mol L Sodium Hypochlorite in 0 1 mol L NaOH e g BDH 23039 e Diluted 3 5 mol L nitric acid e Two screw caps with membrane Procedure 1 Fill the vial to the right Calibrator position with 10 Sodium Hypochlorite solution 2 Fillthe vial to the left Reagent 1 position with diluted nitric acid 3 Close the vials with the screw caps Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 11 Ciruit board list 8001870 8001833 8002603 8002338 8003152 8003175 8003210 8003189 8001871 8002674 8001923 8001705 8001758 8003323 8003227 PBA CMA 600 Heat PBA CMA 600 LED PBA Display adapter New PBA ISCUS Backlight unit PBA ISCUS Flex Bottle sensor PBA ISCUS Flex Connection PBA ISCUS Flex Vial Sensors PBA ISCUS Flex Wash Basin PBA ISCUS Photo diode PBA ISCUS Light switch PBA ISCUS Syringe pump PBA ISCUS Home X PBA ISCUS Sensor Z PBA Main Board ISCUS 4 PBA Z motor If you need specific information of any circuit board please contact CMA Service Department Issued June 2010 11 1 CMA M
36. high resistance spots 4 Startthe ISCUS and enter the service software mode and open the service tab 5 Start the sensor check and check that all sensors in the XZ module works correctly 6 Ifthere are still position errors from the module replace the complete motor and encoder for that specific movement Replacement of the XZ module 1 Remove the main cover of the ISCUS analyser See how to remove the main cover Unlock the two screws at the bottom of the XZ module and one screw on the support arm Remove the cannula and the peek tubing from the cannula holder Disconnect the cables to and from the XZ module Remove the XZ module from the chassis Place the new XZ module into the chassis NN Es A D Reassemble in the opposite order 9 9 Check Replacement of Main board Check of main board 1 Check that all cable connections are connected properly Check that it has not been any leakage on the board from rinse fluid or old reagents Check that the Smart Media card and the small memory battery are installed on the board Check the syringe pump functions by following Check of syringe pump module Check the functions of the rinsing fluid system by following Check of Rinsing fluid system 2 3 4 Checkthe XZ motor functions by following Check of XZ module 5 6 7 Start up the system and check that the windows software and ISCUS software starts up correctly Replacement of main board Warning Use ESD pr
37. ic gases A possible explosion hazard exists and personal injury or equipment damage could occur e The analyser shall not be exposed to direct sunlight nor be placed in a draughty environment e To shut down ISCUS before any transportation the Turn off button must be used Follow the instruc tions on the screen empty wash waste bottles remove reagent cassette and microvials Never trans port ISCUS with fluids remaining inside e The analyser shall never be transported if the transport conditions are to less than 0 degrees Celsius 32 degrees Fahrenheit e Use only CMA Microdialysis spare parts accessories and consumables e All service shall be perform by CMA Microdialysis authorised personnel e Handling of samples shall follow hospital infection risk procedures e WARNING Class 1 LED product LED placed in the detector module of ISCUS Radiation when open do not view directly or with optical instruments e The use of ACCESSORIES transducers and cables other than those specified with the exception of transducers and cables sold by CMA Microdialysis AB as replacements parts for internal components may result in increased EMISSIONS or decreased IMMUNITY of ISCUS ISCUS should not be used adjacent to or stacked with other equipment If adjacent or stacked use is necessary ISCUS should be observed to verify normal operation in the configuration in which it will be used Issued June 2010 CMA Microdialysis Service Manual IS
38. ice Manual 80038664 Issued June 2010 3 12 8002650 Syringe pump Module ISCUS Service Manual 80038664 Issued June 2010 3 13 8002650 Syringe pump Module ISCUS Service Manual 80038664 Issued June 2010 ISCUS Service Manual 80038664 3 15 8002650 Syringe pump Module a e INN Issued June 2010 8002650 Syringe pump Module ervice Manual 8003866A Issued June 2010 ISCUS Service Manual 80038664 3 17 8002650 Syringe pump Module Issued June 2010 ISCUS Service Manual 80038664 8002650 Syringe pump Module nm m FE O Issued June 2010 8002650 Syringe pump Module ISCUS Service Manual 80038664 Issued June 2010 3 19 ISCUS Service Manual 80038664 3 20 8002650 Syringe pump Module Mz SECTION B B SCALE 1 1 Issued June 2010 ISCUS Ser
39. icrodialysis Service Manual ISCUS 8003866A 12 Cable connections Internal cables and connections for ISCUS Flex RI a LL A eet ee Z motor XZ 8002396 8003227 J3 8003175 J3 XZ module conn cable Z flat Z flat cable 8001895 8003227 J2 8001758 J1 XZ XZmodue XZ cable 8002513 8003323 con 12 8003175 J2 E MEN on XZ conn board 8002515 8003323 con 61 8003175 J12 Fixed on XZ conn board J13 J14 Wash syringe 8003323 con 62 8002650 Syringe pump module pump motor Wash motor Wash syringe 8002464 8001923 J3 8002650 Syringe pump modul pump encoder Wash encoder Sample syringe 8003323 con 62 8002650 Syringe pump module pump motor Sample motor Sample syringe 8002464 8001923 J4 8002650 Syringe pump modul pump encoder Sample encoder Rinsing bottles 8002509 8001923 J5 8003152 J1 Fixed on PCB sensors pup Fluid lever sens STA 8001923 J2 8001970 Rinse Waste module Issued June 2010 12 2 CMA Microdialysis Service Manual ISCUS 8003866A Syr pump sens 8002520 8003323 con 13 8001923 J1 Fixed on CB Return pump 8003323 con 62 8002650 Syringe pump module ca ble fixed on pump Solenoid valve 8003323 con 62 8002650 Syringe pump module fixed on valve 8002454 8003323 con 25 8001948 Cable to Printer to Printer p 8003323 con9 8001894 a fixed to Fan Main power sw power sw 8002468 8002605 80018771 Power Power supply module module Power nn 8
40. ing the power supply to the chassis 7 8 9 Remove the power supply from the chassis 10 Place the new power supply into the chassis 11 Reassemble in the opposite order Issued June 2010 9 12 CMA Microdialysis Service Manual ISCUS 8003866A 9 13 A A NA Pe Ugo c9 9 11 Replacement of Fan Remove the rear cover of the ISCUS analyser Remove the screws for the syringe module and lift it out from the chassis still hanging in the cables Remove the transparent cover from the top of the power supply Remove the power supply see replacement of power supply Remove the screws for the house of the power supply and fan Disconnect the Fan cable Pull out the rivets which are holding the Fan to the housing Place the new Fan into the chassis Reassemble in the opposite order 9 12 Check Replacement of Rinse Waste module Check of Rinse Waste module 1 2 3 Open the service software mode see chapter 6 and the Module test tab Start the Chassis Cover test and follow the instructions on the screen This test will check all switches for the rinse waste module Replace the circuit board if any of the switches are broken Replacement of the Rinse Waste level sensors 1 2 Remove the main cover of the ISCUS analyser See chapter 9 1 How to remove the main cover Remove the screws which are holding the syringe pump module Lift out the syringe pump module still hang ing in the
41. ll sensors by activating them manually Here is a list of all the sensors 1 Vial Holder home position Active Motor X Home Active Cannula Sensor Not active Motor Z Top Active Reagent Rack inserted Active Rubber stopper sensor bar pushed Active Reagent home position Active Not in use TEN Soho E GPS Not in use o Not in use Notin use N Vial 1 inserted Active W Vial 2 inserted Active Si gt Vial 3 inserted Active un Vial 4 control sample inserted Active gt mm Sample Syringe empty Active gt SQ Waste bottle inserted Active oo Rinsing fluid bottle inserted 2 Active gt SO Rinsing W aste bottle sensors lever down Not active N Wash syringe in home position Active N Rinsing fluid bottle empty Active N N Waste bottle full Active N W Cannula Wash bin empty Active N ER Not in use N n Notin use Replace Cannula Press Replace Cannula and follow the instructions on the screen Replace Syringes Press Replace Syringes and follow the instructions on the screen Print Calibration Factors A calibration report will be printed where the values of the two calibration points of each analyte will be presented Issued June 2010 ISCUS Service Manual 80038664 Go to all positions Open the Production Step 4 tab and press Reagent and Ch
42. lly done after a change of the detector cell 0 5ul repeatability with vials 2 x 20 samples require very high demands of the instrument 0 2ul repeatability with vials 1x 20 samples normally done after the maintenance Carry over usually done by CMA Production test dept Glucose usually done by CMA Production test dept Tap on the test if you want to skip one or more of them If you want the ISCUS to auto fill the vials mark the check box Auto fill vials To start the test press Run tests Follow the instructions on the screen After inserting vials press OK The result of the test will appear on the screen The tests will stop automatically before it is ready if the result will be out of range The test will then have to be restarted See more info in the chapter of Maintenance service A cleaning procedure can also be run from this tab Insert the Cleaning Cassette and press Reagent and change and choose Cleaning Then OK The cleaning will now start The cleaning rack contains A bottle of Sodium Hypochlorite and a bottle of Nitric acid See the chapter cleaning of instrument Production Step 5 14 Send the result to CMA 15 Printreport on the printer 16 Resetservice flag Sets database intranet Latest 2003 01 01 service to current date time 17 Backup database 2 on next start up Print All Reports Backup does NOT exist Send Rep
43. make it easier to find out info about the problem You can either print out the error list by pressing Print Service Log or look at the files in the instrument 2 Decode any error messages that may occur by the help of How to Decode the Iscus Logfile Chapter 8 In the decoder instruction there are suggestions of which module and movement that has to be checked Example This is a part of a printed log file The first rows give us the following info TS Total Start ups AC Analyse Cycles RC Rinsing Cycles CR Cleaning Racks TH Total Hours In the first column the dates are shown the second column the times are shown and in the third method nr and the error Within the brackets is the number of currencies during that minute are shown The error codes 2 and 5 are frequently represented in the log file example 2 stands for method and 5 stand for error Look for method 2 and error no 5 in the How to decode the ISCUS Log file where you can read Cannula sen sor photo interrupter is unexpectedly activated Under measures you can read Check for obstructions Check Z conveyer belt Check Z photo interrupter and connectors You can also check this Check the Z sensors Sensor Check in the service software Check the slide bearings for the Z movement Check replace the Z motor Follow the above procedure for all mechanical errors in the ISCUS Issued June 2010 ISCUS Service Manual 80038664 7 2Calib
44. mple text file that contains a number of short log entries one line Each entry has a timestamp a method number and an error number The method and error numbers can be decoded using the tables below YYMMDD HH MM Method Error Entries in the log file are automatically deleted when the log file reaches a given size so that the newest log entries are preserved The error numbers The table below holds the error numbers that occur in the log file Error Description 1 Parameter s out of range 2 Home switch activated 3 Position error a position sensor is unexpected Active 4 Position sensor timeout Possibly a FPGA error 5 Cannula sensor is unexpected High 6 Reagent door open 7 Reagent not inserted properly 8 Vial door open 9 Vial not inserted properly 10 Encoder timeout 11 Deviation between the encoder counter and the FPGA position counter 12 X AXIS STEP MOTOR active 13 Z AXIS STEP MOTOR active 14 SYRINGE STEP MOTOR active 15 VIALS in LOAD POSITION 16 Not used 17 Application error 18 Z AXIS home sensor is not activated 19 ISCUS configuration file error wrong format 20 ISCUS configuration file can t be found or opened 21 Home sensor not activated 22 The Wash pump is active 23 The DC motor is already active 24 An undefined combination of sensor states has occurred 25 Only one analog input at th
45. n Remove the inspection cannula lid and follow the instruc tions on the screen Push the release handle on the right side of the cannula holder and remove the old cannula If the old cannula is supposed to be used again Check the flow of the cannula by connecting it to a syringe filled with destilled water and flush the cannula Check that the old cannula is straight and the tip is undamaged If the old cannula does not fulfil these criteria replace it Insert the new cannula First place the sharp end of the new cannula into the cannula guide Then lift and push up the ferrule end of the new cannula until it locks in the correct position The ISCUS will now perform two calibrations the second one after 30 minutes This is also a way to do if you want to calibrate twice independently of the calibration interval In service software mode 1 we cbe ME Press the service tab Press the Replace Cannula button Remove the inspection lid by hand Remove the cannula from the holder If the old cannula is supposed to be used again Check the flow of the cannula by blowing it or connect it to a syringe filled with distilled water Check that the old cannula is straight and the tip is undamaged If the old cannula does not fulfil these criteria replace it Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 9 7 10 Insert the new cannula in the holder Push up the cannula until it locks in the c
46. nt rack motor encoder does not move 21 REAGENT HOME Check for obstructions POSITION P koi ne Check conveyer belt rupter is not active when the movement is com Check photo interrupter pleted and connectors 55 GoToHom Moves the vial rack motor to the 4 Timeout Check vial conveyer eVial home position The photo inter belt rupter VIAL HOME POSITION Vi tek dome photo A a interrupter not activated Check vial home photo defines the home position interrupter and connec tors 7 The vial rack hit the bar when going inwards 10 Timeout Check motor encoder Vial rack motor encoder AUS connectors does not move 21 VIAL HOME Check for obstructions POSITION P note ur Check conveyer belt rupter is not active when the movement is com Check photo interrupter pleted and connectors 56 MoveRela 1 The command would Report to CMA tiveReagent result in a position out Check deantian ob pe side the valid area m sitions in the database 2 REAGENT HOME Check for obstructions POSTION p HOw nie Check conveyer belt rupter is activated dur ing movement Check photo interrupter and connectors 7 The reagent rack hit the bar when going inwards 10 Timeout Check motor encoder Reagent rack motor encoder does not move and connectors Issued June 2010 8 11 CMA Microdialysis Service Manual ISCUS 8003866A Internal es Short Description 2 Error M
47. or each analyte Issued June 2010 5 1 ISCUS Service Manual 80038664 6 Service software mode The service software mode is developed for maintenance testing and troubleshooting the ISCUS Flex ana lyser To activate the service software enter the cogwheel menu and mark the checkbox Maintenance Click on Ser vice code and type the following 1 shift A 2 shift B and 3 OK the system will restart and the following menu appears To reach the higher service level type service code main or dart in the field for service code and press OK Production Step 1 Serial Number texte ox IscusWorkstatn Here follows an explanation of each of the buttons on the screen 1 Adjust touch Makes a calibration sequence of the touch screen Just follow the instructions on the screen Press once on the screen when the calibration is ready to confirm settings If the screen is totally out of alignment and it is not possi ble to reach the correct buttons on the screen an ordinary keyboard can be connected to the ISCUS by the USB port on the left side 2 Set Serial No If you want to type in the serial number once again if the data have been lost after reloading the software See Trouble shooting Normally entered only in production as it is a factory setting It should be the same as printed on the S N label on the left side of the ISCUS Issued June 2010 ISCUS Service Manual 80038664
48. orrect position Put the inspection lid back Then close the lid by pressing End Change cannula If you have a reagents cassette inserted the ISCUS will now perform two calibrations the second one after 30 minutes 9 3 Check Replacement of syringes Sample syringe 500 ul the left one from the backside 1 2 10 11 12 13 Remove the handle and the rear cover of the ISCUS analyser Check that the syringe does not have any leakage If there is any liquid between the piston and the glass tube replace the syringe Enter the service software mode and press the Service tab Press Replace syringes The syringes will now go down so it will be possible to remove them Disconnect the syringe piston from the drive module Just turn the hex nut on the syringe piston counter clockwise and unscrew it Disconnect the syringe body from the upper holder Examine the syringe for cracks or leakage between the glass tube and the piston Fill the new syringe with rinsing fluid and make sure that no air bubbles are visible in the syringe before at taching it to the ISCUS Mount the new syringe into the upper holder tighten it gently with a plier A too hard tightening can cause damage to the syringe glass body and plastic threads Mount and secure the syringe piston into the drive module Press End replace syringe The syringes will now move to the adjustment position Unlock the insex screw which locks the s
49. ort StartService GUI Once Again Issued June 2010 ISCUS Service Manual 80038664 6 6 14 Send the result to CMA database intranet Press Submit Result Only for internal use 15 Print a report on the printer Press Print Report when you want the ISCUS to print the test results on the printer 16 Reset the service flag Press Service performed to reset the service flag A new warning for old syringes and cannula will show next time after 13 months 17 Backup database on next restart Press Backup DB and the database will be backed up at next start up Shut Down Press shut down to make ISCUS shut down correctly Remove reagents vials and press Lock the instrument will now shut down and lock Lock This is not a recommendable way to turn off the instrument A faster way to shut down the instrument without removing any liquids Warning The instrument will not evacu ate the wash liquid from the wash basin Start Service GUI Once Again Ifthe check box Start Service GUI Once Again the instrument will start up in Service mode directly after next restart Service tab Wash x 10 Press Wash x 10 to make the syringes wash 10 times Issued June 2010 ISCUS Service Manual 80038664 Wash Press Wash to make the syringes wash once Start Sensor Check Start the sensor check by pressing Start Sensor Check While this sensor check is active it is possible to check a
50. otection before working with the mainboard 1 Remove the main cover of the ISCUS analyser See instruction Remove the XZ module See Replacement of XZ module Remove the radiation shield and the detector module See Replacement of the XZ module Disconnect the cables to and from the main board Remove the mounting screws for the main board and adjustment screw for the monitor Qv Uode ID Remove the main board from the chassis Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 7 Place the new main board into the chassis 8 Reassemble in the opposite order 9 10 Check Replacement of Power supply Check of the Power supply 1 Check that all cable connections are correctly connected to the power supply 2 Startup the ISCUS and measure the voltage on Con 29 on the Main board CON29 Power supply unit No Name REF DES 1 12V 12V 2 45V 45V 3 5V 5V 4 5V 5V 5 GND GND 6 GND GND 7 GND GND 8 GND GND 9 12 V 12 V 10 24 V 24 V 3 Ifthere are not any voltage levels on these test points please check the fuses in the main inlet Replacement of the Power supply Remove the rear cover of the ISCUS analyser Remove the transparent cover Disconnect the cables from the top of the power supply first and then the lower connection Remove the ground connection from the power supply 4 5 6 Remove the four mounting screws T 20 which are hold
51. otor encoder position This means and connectors that the motor did not manage to move to the desired position 18 Z home photo inter Check z photo inter rupter is not active X rupter and connectors movement is forbidden so that the cannula is not ZN broken 5 MoveRela Moves the cannula up down 2 Z HOME POSITION Check photo interrupter tiveZ photo interrupter is un and connectors expectedly activated 5 Cannula sensor unex Check for obstructions pected activat Check photo interrupter and connections 10 Timeout Check for obstructions Z motor encoder does Check motor encoder not move and connectors 11 Deviation between the Check for obstructions Au rosier Check conveyer belt position and the ex pected motor encoder Check motor encoder position This means and connectors that the motor did not manage to move to the desired position 6 MoveRela Moves the pipetting syringe as 2 SYRINGE HOME Check photo interrupter tiveSyr pirate dispense POSITION photo inter and connectors rupter is unexpectedly activated 10 Timeout Check for obstructions Pipetting syringe motor encoder does not move Check motor encoder and connectors Issued June 2010 8 7 CMA Microdialysis Service Manual ISCUS 8003866A Internal E Short Description 2 Error Measures e Name S 3t 11 Deviation between the Check for obstructions
52. ple rate for the analog input de vices is longer then the Timeslot value that is used to calculate the average value Report to CMA 31 Run WastePump Runs the waste return pump DC motor that empties the washbin Timeout Waste return pump en coder does not move Check the wash bin sen sor wipe it off Check the waste tubing The DC motor is al ready active Report to CMA 43 UpdateCon figFiles Reads the hardware configuration file ISCUS configuration file error wrong for mat Check file integrity Report to CMA 20 ISCUS configuration file can t be found or opened Check file integrity Report to CMA Issued June 2010 8 10 CMA Microdialysis Service Manual ISCUS 8003866A Internal 3 Short Description z Error Measures Name 8 3t 26 The MCUConfigura Check file integrity tion file contains error Read the Report to CMA ISCUS_LOG log for more information 54 GoTo Moves the reagent rack motor to the 4 Timeout Check reagent conveyer HomeR home position The photo inter dun occi Rome belt eagent rupter REAGENT HOME ger i photo interrupter not Check reagent home POSITION defines the home po j i me activated photo interrupter and sition connectors 7 The reagent rack hit the bar when going inwards 10 Timeout Check motor encoder and connectors Reage
53. ps and 16 empty vials Go back to the Module test tab and press the Start button for the XZR Follow the instructions on the screen A check of the reagents vial and detection bar sensors will be performed The cannula will go to all positions Cell test Insert a production cassette before starting A production cassette contains From the left Amido black 0 26M NaNO2 H 0 Glucose 1450ul H20 Calibrator A But to run the Cell Test it is only necessary to use the H O Destilled Open the Production Step 4 and press Reagent and Change and mark production to load the reagent Go back to the Module Test tab and press Start to start the test The result will be displayed on the Production test 4 tab Syringes Choose how many iterations you want the syringe pump to perform Press Start to make the test sequences for the syringes A message Module test OK will appear if the test was OK Info tab Gives some info about error messages total uptime total amount of cycles and chassis temperature Total Startups 148 Analysis Cycles 7863 Rinse Cycles 8838 no error Cleaning racks 0 Total Uptime 1602h 4 27 2006 1 04 10 PM Class TemperatureRI ChassisTemperature 28 5 State tsMediumLow Issued June 2010 ISCUS Service Manual 80038664 7 1 7 Trouble shooting 7 1 Hardware faults 1 Gather all information you can found out about the problem Start asking the operator to
54. r then the Timeslot value that is used to calculate the average value Report to CMA 23 GetRefVolt age 25 Only one analog input at the time is allowed to execute Report to CMA 27 The sample rate for the analog input de vices is longer then the Timeslot value that is used to calculate the average value Report to CMA 24 Get5V 25 Only one analog input at the time is allowed to execute Report to CMA Issued June 2010 8 9 CMA Microdialysis pou Internal Name Service Manual ISCUS 8003866A Short Description 10 117 Error Measures 2 SI The sample rate for the analog input de vices is longer then the Timeslot value that is used to calculate the average value Report to CMA 25 Get24V 25 Only one analog input at the time is allowed to execute Report to CMA 27 The sample rate for the analog input de vices is longer then the Timeslot value that is used to calculate the average value Report to CMA 26 Get0V 25 Only one analog input at the time is allowed to execute Report to CMA 27 The sample rate for the analog input de vices is longer then the Timeslot value that is used to calculate the average value Report to CMA 27 GetTemp 25 Only one analog input at the time is allowed to execute Report to CMA 27 The sam
55. ration control samples or normal samples faults Example of logfile If the calibration values are missing like this make the following checks 1 Check replace Cannula 2 Check all connections to the Peek tubing Check that the connections are tightened correctly so that no air will let into the liquid system Make a cell test to determine if the liquid system is tight 3 Check replace detector cell 4 Check replace sample syringe If the syringe is older than one year or if there is a leakage between the piston and the glass we recommend that you replace it Issued June 2010 7 2 ISCUS Service Manual 80038664 5 Check replace wash syringe 6 Check replace valve solenoid 7 Check replace sample syringe pump driving 8 Check replace detector module 9 Check replace wash syringe pump driving 10 Check replace main board Below is a part of another printed log file Here follows an explanation of the codes BL Blanks CI Calibration Injections CL Calibrations BB Bad Blanks BCI Bad Calibration Injections Here you can get some statistics from the calibrations From the above statistics you can read that there has been one bad blank measurement There has been 6 good calibrations out of total 6 calibrations despite two bad calibration injections Issued June 2010 7 3 CMA Microdialysis Service Manual ISCUS 8003866A 8 How to decode the ISCUS log file The Iscus log file is a si
56. scription Qty 19 8001814 Isolation pad 1 20 8001812 Heating block 1 21 8001973 Screw MRT 3x6 A4 2 Not nr M000032 Nut KEL F 1 Not nr M000033 Ferrule KEL F 1 Issued June 2010 8002190 Detector module ISCUS Service Manual 80038664 Issued June 2010 3 24 ISCUS Service Manual 80038664 8002190 Detector Module parts list Position CMA P N Description Qty 1 8001824 Detector housing 1 2 8001808 LED module 1 3 8001804 Photo diode module 1 4 8001870 PCB CMA Heat 1 5 8001827 Sensor cover 1 6 75403005 Screw MRT 3x5 A4 4 7 8001829 Spacer DRN 3160x3 4 8 75403010 Screw MRT 3x10 A4 4 10 8002270 Cable LED module 1 11 8002269 Cable Photo diode module 1 12 8002175 Sticker Class 1M LED 1 14 8001946 Peek holder 1 15 8001945 Peek bracket 1 16 8001973 Screw MRT M3x6 8 17 8003313 Washer FBB 3 1 8 18 75140320 3 2Washer AZ lock 4 Issued June 2010 ISCUS Service Manual 80038664 8003203 Rinse Waste Module Issued June 2010 3 27 ISCUS Service Manual 80038664 8003203 Rinse Waste level sensors Issued June 2010 3 28 ISCUS Service Manual 80038664 8003203 Rinse Waste Module Issued June 2010 ISCUS Service Manual 80038664 8003203 Rinse Waste Module parts list Position CMA
57. st according to the service software mode Service tab Go to all positions Run Cell test Load a cassette with destilled water in the third position Put in the cassette by pressing Re agents and then Change and choose Production Open Module Test Start Cell Test Check the re sult in Step 4 Run the RSD test Repeatability with vials 0 2ul Select Step 4 tab Load a cassette with Amido black in the first position from the left and water in the second position Put in the cassette by pressing Reagents and then Change and choose Production Skip the optical repeatability with vials 0 5ul carry over and glucose test by pressing them once Press Run tests Insert four empty vials Press OK and the test will start Make sure the results are within correct levels If results are not within correct levels restart the test again but pre fill four vials with Amido black and clean out the auto fill square If the test still does not work check the cannula and also check that air bubbles are not present in the sample syringe When the test is OK reset the service flag turn off ISCUS and restart in normal mode Load the analyser with reagents and calibrate the system Run control samples Put in a vial in the CS position in the vial holder and analyse the sample Press the Con trol Samples button to look at the results Make sure that the results are within the range f
58. t If the signal levels for VIS and UV are below 1000 mV with the maximum output it may help to increase the signal level if performing a cleaning of the detector cell See chapter 10 Cleaning instruction Check also that there is not any leakage from the cell due to a broken glass tube in the cell Make a calibration Print the log file and check that the water values are stable better than 0 3 deviation Replacement of the Cell 1 a E a D Remove the main cover of the ISCUS analyser Remove the cannula See replacement of cannula instruction Remove the peek tubing from the cannula holder Remove the peek tubing from the valve solenoid on the syringe pump module Remove the mounting screws for the detector cell Remove the detector cell from the detector unit Reassemble in the opposite order Start up the instrument and make a centre volume calibration See Service software mode Issued June 2010 9 9 CMA Microdialysis Service Manual ISCUS 8003866A 9 7 Check Replacement of Detector module Cuvette Photodiode Inlet tubing PR LED s Outlet tubing Pre amplifier 530 or 375 nm Optical Filter Lens mum D ae Sce TA IIA 4 B RE Al Plastic cone 1 Light beam T kal Pi Quartz capillary Figure 1 Principle of the optical system The optical system of ISCUS is equipped with a single beam filter photometer Two Class 1 Light Emi
59. the syringe piston up until it hits the seat of the syringe holder Then back the piston down one complete turn before the insex screw is locked again 12 Press End adjust Syringes The syringes will now make a flush 13 Mount the rear cover and the carry handle of the ISCUS analyser 9 4 Check Replacement of Syringe pumps modules Check of the Syringe pump module 1 Open the service software mode and enter the Service tab 2 Press either Wash x 10 or Wash to check that the wash syringe is working properly 3 Check for air in the syringe If there are large air bubbles in the syringe even after several flushes check that the syringe is tightened correctly and no cracks are visible in the glass body The air can also depend on a faulty solenoid valve 4 Press Replace syringe and the syringes will go down and make it easy to check if any bubbles are present on top of the pistons Then press End replace syringes and End adjust syringes to get back Replacement of the Syringe pump module 1 Remove the rear cover of the ISCUS Flex analyser 2 Remove the Peek tubing connectors from the syringe pump module Rinse fluid bottle valve solenoid and detector sample syringe Disconnect the cables for the syringe pump module Remove the mounting screws for the syringe pump module Remove the syringe pump module Place the new syringe pump module and secure it with its mountings screws Connect the
60. tting Diodes LED with wavelengths of 530 and 375 nm receptively are used as the light source The active LED 530 or 375 nm is determined by the analytical method The light beam passes through an optical filter and then through the cuvette Passing through the optical lens the light beam then reaches the photodiode where it is converted to an electrical signal The electrical signal is pre amplified in the detector unit before the signal is passed to the analogue to digital converter The measuring cuvette is designed to minimise the risk of getting air bubbles trapped The light beam enters the cuvette through a plastic cone and is reflected by total internal reflection into the quartz capillary Then it passes through the quartz capil lary containing the sample in a zigzag pattern also by total internal reflection between quartz and air The effective volume of the cuvette is about 2 uL and the geometric path length 10 mm The effective path length is shorter how ever about 8 mm since a part of the travel through the capillary occurs in the quartz wall The temperature of the optical unit is controlled to 37 C Check of the detector 1 Print out the service log and check the water levels or open the service software mode and the Step 3 tab 10 Adjust the LED light levels Press Start and check the signal levels for VIS and UV 2 Are the water signal levels higher than 1000 mV the ISCUS is capable of running analyses If th
61. uctions actual motor encoder position and the ex pected motor encoder position This means that the motor did not manage to move to the desired position Check conveyer belt Check motor encoder and connectors Issued June 2010 8 8 CMA Microdialysis Internal Name Short Description 10 117 Service Manual ISCUS 8003866A Error Measures POPIN GoTo HomeWash Moves the wash syringe to the home position The photo interrupter WASH HOME POSITION defines the home position 4 Timeout WASH HOME POSITION photo inter rupter not activated Check Z conveyer belt Check photo interrupter and connectors 10 Timeout Wash syringe motor encoder does not move Check for obstructions Check motor encoder and connectors 21 WASH HOME POSITION photo inter rupter is not active when the movement is com pleted Check for obstructions Check conveyer belt Check photo interrupter and connectors 21 GetDetecSig 25 Only one analog input at the time is allowed to execute Report to CMA 27 The sample rate for the analog input de vices is longer then the Timeslot value that is used to calculate the average value Report to CMA 22 GetDetec Temp 25 Only one analog input at the time is allowed to execute Report to CMA 27 The sample rate for the analog input de vices is longe
62. vice Manual 80038664 8002650 Syringe pump Module parts list Position CMA P N Description Qty 1 8001912 Pump bottom plate 1 2 8001917 Pump side left 1 3 8001918 Pump side right 1 4 8001919 Pump top plate 1 5 8001878 Syringe adapter wash 1 6 8001913 Syringe adapter sample 1 7 8002752 Solenoid valve 1 8 8001915 Return pump 1 9 8001908 Drive screw complete 2 10 8001916 Tooth belt 79TN15 2 11 8001923 PCB ISCUS Syringe pump compl 1 12 8003314 Washer FBB 4 1 8 13 8001475 Screw MRT M4x12 8 14 8002525 Screw MRT M3x25 4 15 8001835 Screw MRT 3x8 4 16 8002213 Screw MRT 2x6 2 17 8001911 Screw UC6S A2 UNC 6 32x13 6 18 75403010 Screw MRT 3x10 2 20 8002214 Pump support 1 21 8003313 Washer FBB 3 1 12 22 8001904 Stepper motor complete 2 23 8001875 Tooth wheel compl TN 15240 2 24 8002526 Spacer DRM 3260x10 4 25 D000011 Gasket syringe to syringe adapter 2 26 7431071 Syringe 1 ml for ISCUS 1 27 7431070 Syringe 500 ul for ISCUS 1 28 8002695 Locker for connector 2 29 8002785 Spacer 1 30 8002754 Finger tight Fitting 3 31 8001819 Screw MRT M3x12 6 32 75140320 Washer AZ 3 2 lock 4 33 8003311 Washer FBB 2 1 2 Issued June 2010 3 21 3 22 ISCUS Service Manual 80038664 8002412 Detector cell Issued June 2010 ISCUS Service Manual 80038664 8002412 Detector cell parts list Position CMA P N De
63. yringe adjustment screw Screw the syringe piston up until it hits the seat of the syringe holder Then back the piston down one half of a complete turn before the insex screw is locked again Press End adjust Syringes The syringes will make a flush Wash syringe 1ml the right one from the backside 1 Check that the syringe does not leak If there is any liquid between the piston and the glass tube replace the syringe 2 Enter the service software mode and press the service tab 3 Press Replace syringe The syringes will now go down so it will be possible to remove them 4 Disconnect the syringe piston from the drive module Just turn the hex nut on the syringe piston counter clockwise and unscrew it 5 Disconnect the syringe body from the upper holder 6 Examine the syringe for cracks or leakage between the glass tube and the piston 7 Fillthe new syringe with rinsing fluid and make sure that no air bubbles are visible before attaching the syringe into the ISCUS 8 Mount the new syringe into the upper holder tighten gently with a plier A too hard tightening can cause damaging of the syringe glass body 9 Mount and secure the syringe piston into the drive module Issued June 2010 CMA Microdialysis Service Manual ISCUS 8003866A 9 8 10 Press End replace syringe The syringes will now move to the adjustment position 11 Unlock the insex screw which locks the syringe adjustment screw Screw

SERVICE MANUAL ISCUS Flex 8003200

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