Service Manual - Frank`s Hospital Workshop
Contents
1. Lr Service Manual HUMALYZER 2000 6 9 3 q 9 2 1 Bi 5 21 O 0 Es lo 7 17 O o O 95 16 CO KD Page 35 Diagrams Block Diagram PCB Layouts Main PCB Pump Control PCB Interface PCB Printer PCB Incubation block PCB Printer PCB Incubation block PCB Schematics Main PCB Pump Control PCB Interface PCB Printer PCB Incubation block PCB Service Manual HUMALYZER 2000 Page 36 0002 H3ZA IVINH lenueyy 99 9 18 9 SOLDER LUG 115VAC BURSSMDL 1 2 SWITCHED MAINS TF1 TF2 THERMAL FUSE 218 VOLTAGE SE BLK W No 1 BRN 12 11 FB 2 BRN XQ 4 IS 1 6 BLU 3 1573 3ESIREEBA2. 9 1 vos 037 u 08 141 SNYUL OLONd 5031 3 1 E 69 V 1 2 4 1 1 dS 9 wueoct 1 1 1 4 n QuvOG NOM YNHH3I 01 1 1 did Nid fi 1 M 1 1 1 250566 TT YSLSINOLOHd lt seh rk Page 46 Service Manual HUMALYZER 2000 Rev C Pump Control PCB NNO2 Hiv 1 y i 9 6 1 1 Li LI 1 1 1 24 m 1 044 Vins m p orr nor 01 HOLY MOLY i 3 e x AE 300 Page 47 Service Manual HUMALYZER 2000 Rev C Interface PCB 20 300 1204 1313 n 2 I Y 2 8 005 cU 82d dnvs 031 s 3904 Ind 111111 ii 834 6CISTEL 3b Cun 124 lt 107 54 ov CRY 138905 un 0092 2084 7 34 4 OL Page 48 Service Ma
3. AWARENESS 7 ECHNOLOGY 2500 38 ur Po MSY amoo Hnololdj 101138 Service Manual HUMALYZER 2000 Rev C Pump Control PCB 143 ud ci PRESSURE SENSOR Service Manual HUMALYZER 2000 Page 39 Interface PCB and Parallel Buffer PCB 2100 1004 INTERFACE BOARD PCA 995009 PRINTER POWER gt p THERMAL PRINTER PARALLEL PORT LED SAMP SW LCD DISPLAY FROM MAIN PCB KEYBOARD KEYBOARD PARALLEL BUFFER J8 D825 F s u00 Service Manual HUMALYZER 2000 Rev C Page 40 Rev C Printer PCB 5V MIT Te TG GND HOME 04 PIC16C57 2MHZ RI R2 R3 R4 8 5 05 184108 AWARENESS TECHNOLOGY ORG 300 200C PRINTER INTERFACE P N 184108C MODIFIED ASSY NOTES 2 2 96 ECO 408 413 ALL VERSIONS 1 ADD 0 01uF CAP C9 ON SOLDER SIDE AS SHOWN 2 INSTALL JUMPER WIRE IN PLACE OF D1 STANDARD 995002 1 DO NOT INSTALL Q3 R6 U4 OR Ji 2 DO NOT INSTALL JUMPER WIRE AT 05 GRAPHICS OPTION 995006 1 DO NOT INSTALL J2 2 INSTALL JUMPER WIRE AT 25 3 INSTALL Q3 R6 04 AND 23 Service Manual HUMALYZER 2000 Page 41 Rev C Schematics Main 1 5 area pmo 9 Jiya CAR 11 2 0170 e 031v5 i 0 2 Cr
4. amp 46313152 lortNZ 4 AY3Hi 340 01 3 1 Java 1 rv wow 40v 34 as panys 153130 j aza YOLVIWISO 5 ftu ni tu URN 01 2999 2 0355384 AS 2144 5 1 100 3901 1 DAT MM JBYN3 0 ate lt p 437 gt oot gt ne T a E AIG 533 383111 Page 44 Service Manual HUMALYZER 2000 Rev Main 4 5 45 1 Bod WNAI 821200108401 H 7 west 5 t 2 E Tams i deni ud i dd naut A Casi B 5 vena gt 1 gun Mt 2018 641 1 9 i 9528 1 1 sen ZN Sid 1531 f vcooann 79 merr 3 2 21 57 PT gt 2 wv HOT 58 DUNS NY9 WHOL dO t 1 LER n or 3183 02 2 H S Y d313 O010Hd M LEN Service Manual HUMALYZER 2000 Rev C Main 5 5 2
5. 4 40 screws holding the upper cell body and separate it from the cell bracket 4 Assembly is the reverse of this procedure After assembly complete the procedure Flowcell adjustment to restore optical alignment Service Manual HUMALYZER 2000 Page 16 Valve Tubing Replacement The short length of silicone tubing used in the sampling valve may become clogged or worn with age A replacement is included in the tubing kit 1 Figure 8 Set the power switch to OFF Open the instrument as described in Opening the instrument Refer to Figure 8 A Locate the valve behind and to the right of the photometer Refer to Figure 8 B Pull back the pinch bracket and remove the valve tubing from the valve body Disconnect the valve tubing from the fittings at both ends Install the replacement tubing to the valve body in a similar manner Push the tubing over the tubing barbs until seated Be especially careful not to kink stretch or tension the tubing Carefully lower the instrument cover and replace the screw Service Manual HUMALYZER 2000 Page 17 Vacuum Adjustment To adjust the vacuum you will need Reference vacuum gauge reading at least 18 cm Hg 2 15 cm length of 3 1 mm ID flexible tubing 3 1 mm barbed tee fitting 1 Disconnect the vacuum line blue Luer fitting at the rear panel Open the instrument 2 Refer to Figure 9 A Connect the vacuum gauge as shown Refer to Figure 9 B Locate the pump cont
6. Spectrophotometer Type Optical Configuration Usable Spectral Range 31 May 1994 Humalyzer 2000 Cat No 18300 Filter photometer Single beam with continuously rotating filter wheel Monochromatic or bichromatic reading 8 filter positions 330 to 700 nm System Procedures Open and by stored menu Calculating Modes Channels Absorbance Single Standard Differential samples Factor Mode Differential samples Multi Standard Mode 7 standards Multi Standard 96 Abs 7 standards Kinetic Mode By Factor or by Standard Fixed Time Kinetic By Factor or by Standard 44 fixed 24 open Source of Radiation Tungsten Halogen 10 Watt with automatic lamp saver Selection of Wavelength Filter Type Cuvette Wavelength Accuracy Filter Location Filter Selection Wavelengths Half Bandwidth 1 100 Bandwidth False Radiant Energy Ratio Type Material Geometry Illuminated Volume Minimum Read Volume Service Manual HUMALYZER 2000 By filter 4 cavity interference long life ion beam assisted deposition 3 After sample heat filter before sample Automatic by software or via keyboard 340 405 505 545 580 630 nm supplied standard other additional filters optional 10 nm 14 nm at 340 lt 0 001 at 340 and 405 nm Flow through 316 stainless borosilicate windows Cylindrical 2 3 mm dia x 5 mm 0 05 mm 21 uL 250 uL Page 31 Aspiration Purge Valve Other Vessels Cuvette
7. Allow the solution to remain in the flowcell for 3 minutes 3 Aspirate 15 ml of distilled water then purge with air for 5 seconds 4 Aspirate 0 1N sodium hydroxide NaOH Allow the solution to remain in the flowcell for 3 minutes 5 Purge with air for at least 5 seconds 6 Aspirate 0 1N hydrochloric acid HCI Allow the solution to remain in the flowcell for 3 minutes T Purge with at least 15 ml of deionized water 8 If the flowcell is to be removed for storage purge with air until more fluid can be seen flowing into the waste bottle Otherwise leave the flowcell filled with water Service Manual HUMALYZER 2000 Page 12 Flowcell Tubing Replacement The flowcell utilizes 1 2 mm 1 0 Teflon tubing for the sample and exit tubes Replacement tubing is included with the tubing kit Follow this procedure to replace the flowcell tubing 1 Remove the flowcell Unscrew the Luer and lift the flowcell out of the read well Remove the cover screws and lift off the upper flowcell cover Refer to Figure 5 A Disconnect the tube from the steel tube then remove the exit tube Pull the exit tube out through the bulkhead Remove the cell insert screws and pull the cell insert and the sample tube out Remove the sample tube from the steel tube Select the long or the short sample tube The short sample tube must be used when the sample volume is set to 350 ul or less Carefully press fit the end of the sample tube with the
8. Opening the Instrument Refer to Figure 3 Instrument Interior The cover is hinged at the rear panel and can be raised to allow access to the inside of the instrument Disconnect the power cable the tubing and the sensor lead from the rear panel Move the instrument forward until the front edge overhangs the work surface Locate and remove the cover screw from the underside of the front edge Gently lift the front of the cover upward taking care to clear the incubation block and photometer Prop the cover open with a suitable object Do not force the cover backwards Damage to the cover or fittings may result Service Manual HUMALYZER 2000 Page 11 To reinstall the cover reverse the procedure Carefully lower the cover until it seats on the chassis taking care to clear the incubation block and the Luer fitting Replace the screw Cleaning the Flowcell The flowcell should be cleaned when the instrument will not be used for an extended period e g overnight end of shift and when storing the flowcell Proper cleaning will help to prevent clogging of the flowcell tubing and valve tubing Cleaning is extremely important to obtaining accurate repeatable results If reagent serum or other proteinaceous fluid is allowed to dry in the flowcell it is extremely difficult to remove and its presence can affect test results To clean the flowcell 1 Purge with air for at least 5 seconds 2 Aspirate several ml of flowcell cleaning solution
9. This instrument relies on software calibration that is on calibration data stored in non volatile RAM This data is used by the microprocessor to compensate for variations in electronic components and manufacturing During factory calibration the calibration data is set and any hardware adjustments are made using precision reference standards The calibration data is then recorded on a label located on the bottom of the instrument The factory calibration is very stable and does not drift appreciably over time making trimpot or other component adjustments unnecessary unless the trimpots are disturbed or calibration related components replaced Recalibration of the instrument software or otherwise should not be considered until all possible interfering factors have been ruled out If field calibration is in fact necessary it should only be performed with the proper reference materials and instruments In any case software calibration should be attempted before any trimpot or component replacements or adjustments are considered The temperature calibration data is stored as offsets which the instrument adds to the sensed temperatures Separate offsets are maintained for the incubation block and the read well The absorbance calibration data is stored as a scaling factor which is the ratio of a known reference absorbance to the instrument s reported absorbance The calibration data may be printed at any time by selecting test 213 This prints the
10. an oscilloscope Typical test point waveforms are shown in Figure 4 Figure 4 WAVEFORMS MEASURED AT J8 WHILE READING BLANK 12 mm BOROSILICATE TUBE WITH 1 ml WATER ONE REV OF FILTER WHEEL 340 ms DURING READING Y MINIMUM PEAK HEIGHT 2V 10V m 111 1 1 444 L f 4 4 4 4 4 L 111 11 amp amp gt amp 2222222 Sample M Hold NEL 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 a lt 30m V BASELINE HASH QPTTONAT 4 340 nm FILTERS 630 nm 580 nm 545 nm 505 nm 405 nm Home Service Manual HUMALYZER 2000 Page 7 Internal Printer The internal printer is a 20 column thermal type device which prints ASCII characters using 5 x 7 dot matrix There are no adjustments However a printer jam can occur if the printer paper tears and becomes lodged in the printer mechanism thus preventing the print head from moving freely If the instrument displays Printer is jammed or if the print head does not return to the home position check for obstructions in the print head path CAUTION Do not apply any force to t
11. determines the base of the log 10 for absorbance and is used to adjust the low end absorbance calibration gain Another potentiometer is used to adjust the high end absorbance offset Temperature control The incubation block and read well are maintained at 37 C by means of thermistors 10k 025 These thermistors are multiplexed at 0 5 second rate into the feedback path of oscillator UT4A The waveform at U14A is a sawtooth of approximately 1 2 Vpp amplitude centered at 2 5 V Schmitt triggers U11 convert this to a square wave for current reversal and to drive the CLK2 input of U4 The microprocessor heats the system to maintain the frequency of this signal at 2 08 kHz the 37 C set point Power resistors located on the bottom of the incubation block are switched to 115VAC via an opto triac A red LED located on the block PCB illuminates when heat is on A similar system is employed for the read well except that driver U10 applies 12VDC raw across heater resistors located on the cell PCB Service Manual HUMALYZER 2000 Page 3 Flowcell and Vacuum Pump An integral DC vacuum pump maintains a constant vacuum on an external waste bottle by means of a solid state vacuum sensor circuit on the pump control PCB A solenoid operated pinch valve is opened for a precise interval under microprocessor control to apply this vacuum to the flowcell Sample or air is drawn through the flowcell and into the waste bottle Sensor wires built into
12. is not twisted Install the photometer PCB taking care to center the board around the shaft Service Manual HUMALYZER 2000 Page 21 Replace the photometer cover taking care to position the cable in the slot of the cover Do not pinch the cable under the cover Tighten cover screws only until snug Do not over tighten the photometer cover screws Install the photometer subassembly to the chassis by replacing the 3 6 self tap screws through the chassis base Line up photometer with locating marks made in step 2 Connect the cables removed in step 1 Attach the power cord and connect the instrument to the mains supply Set the power switch to ON 1 Select test 186 The displayed numbers represent the voltages of the installed filters All voltages should be between 2 and 11 volts iat Service Manual HUMALYZER 2000 Page 22 Index hole filter position Home position Filter Label The filter label located on the photometer cover describes the specific filter wheel configuration for your particular instrument Refer to Figure 12 Filter wheel position POS is the physical placement of the filter on the wheel The filter wheel position numbers are shown in the drawing Each filter position may have several filter elements installed Filter configuration WAVELENGTH column des
13. serial number of the instrument the date of last calibration and the stored calibration data for the absorbance block temperature and cell temperature The calibration data is stored with a check sum that is recalculated and compared each time a mode is selected Failure to recover the calibration data properly checksum failure will be indicated on the display and the internal printer Do Temp Test 210 Do Abs Test 212 The instrument annunciates this condition with multiple beeps The temperature offsets for both the incubation block and the read well are then set to 0 0 and the absorbance scaling factor is set to 1 000 In this condition the maximum temperature error is 1 5 C and the maximum absorbance error is 10 The calibration data can be restored as described in Restore calibration The hardware calibration of the instrument in time is based on a 2 MHz ceramic resonator RES1 Calibration in temperature is based on C24 0 0684 polystyrene capacitor trimpots TP4 and TP5 and time Calibration of absorbance is based on C16 a 0 11 polystyrene capacitor trimpots TP2 and and time Service Manual HUMALYZER 2000 Page 25 Calibration and Absorbance Linearity Each instrument is calibrated during manufacturing using standards that are traceable to the National Institute for Standards and Testing NIST and is tested to verify its linearity to 2A This preset calibration is very stable Absolute calibration can be ve
14. the accuracy of the instrument Follow these steps to restore the electronic calibration 1 Shut off the instrument Remove tubes or cuvettes from the incubation block and read well Carefully lift up the instrument and locate the Calibration Data label on the underside of the unit There are three values recorded there Absorbance Block Temp and Cell Temp Write down these numbers Set the power switch to ON 1 If the date and time are incorrect set the correct date and time Press TIME then press EDIT Enter the date and time as prompted Press MENU type in 210 and press ENTER When the display shows Block enter the number that is recorded under the Block Temp heading on the calibration label For example 0 3 or 0 2 and then press ENTER The display will prompt Cell Enter the data from the Cell Temp line of the calibration label Press MENU type in 212 and press ENTER When the display shows Abs Factor enter the number from the Absorbance line of the calibration label If the message Adjust Out of Range is displayed check your values and repeat this step Press MENU type in 213 and press ENTER to get a report of the calibration data The block and cell temperature adjust will be printed along with the absorbance adjustment Make sure that these values are the same as those recorded on the calibration label Service Manual HUMALYZER 2000 Page 27 Restore Filter Labels Like the calibration d
15. 2 no adjustment is needed If the displayed value is less than 50 greater than the value in step 2 Remove the flowcell Adjust the set screw with the hex wrench supplied Turn the set screw 1 4 turn in either direction and go to step If the value in step 4 increases turn the set screw in the same direction If it decreases turn the setscrew in the opposite direction Repeat steps 4 and 5 until the displayed value is at a maximum When complete press CLEAR twice to return to the main prompt You must read new water values as described in Flowcell Configuration Service Manual HUMALYZER 2000 Page 15 Flowcell Disassembly The flowcell may be disassembled for extensive cleaning or replacement of the cell insert To replace the cell insert 1 Follow the procedure for Flowcell Tubing Replacement except install new cell insert before beginning step 6 2 Install the new cell insert in the same orientation as the old Do not force the new cell insert into the lower cell body The part should slide easily into place If it does not fit easily rotate the part 180 Install the 2 2 56 screws that attach the flowcell insert to the lower cell body 4 Complete the procedure Flowcell adjustment to restore optical alignment To completely disassemble the flowcell 15 Follow the procedure for Flowcell Tubing Replacement through step 3 2 Remove 2 2 56 screws holding the lower cell body and lift it off 3 Remove 2
16. ABEE Fans 23 GALIBRATION 25 CALIBRATION AND ABSORBANCE LINEARITY 26 RESTORE CALIBRATION DATA sa eier aan ze aa aaa 27 RESTORE FILTER EABELS tases Roa 28 Service Manual HUMALYZER 2000 Page 1 TEMPERATURE CALIBRATION 29 ABSORBANCE CALIBRATION tv 30 zelda c r 31 REPLACEMENT RAR TS Heer e x Eb Ula 33 BINEI RAIS E 36 DIAGRAM e 36 E S OUR ccc INR 36 MAIN f 36 PUMP CONTROL CU cece es 36 INTERFACE PO Br ND ER 36 PRINTER t 36 INCUBATION BEOGI POB a 36 PRINTER c 36 INCUBATION BEOCK sense eek 36 SCHEMA TIOS a 36 MAIN OB ee ae ox Sr oe eee 36 CONTROL POB P 36 INTERFACE ER 36 PRINTER needs 36 INCUBATION BEOG Raa 36 Service Manual HUMALYZER 2000 Page 2 Introduction The instrument is a general purpose bichromatic photometer system with six available wavelengths and 37 C incubation Two additional wavelengths are optional for a total of
17. Holder Detector Signal Processing and Display Display Type Scale of Display Absorbance Concentration Kinetic Results Zero Compensation Range Signal Outputs Parallel Serial Spectrophotometric Inaccuracy Flow through Stability Warm up Time Electronics Power Supply Dimensions and Weight Space Requirements Service Manual HUMALYZER 2000 Vacuum pump at 18 cm of Hg Silicone pinch type 12 mm test tubes 1 cm square cuvettes Thermostatically controlled compartment at 37 C Gallium Arsenide Phosphide photodiode 2 line x 24 character super twist LCD 0 5 to 3 5 flow through mode 0 5 to 2 5 tube or 1 cm cuvette Maximum 999 999 Abs min with resolution of 0 0002 A min Automatic 0 5 to 2 0 absorbance Centronics IBM PC compatible RS 232 at 2400 baud 8 data 1 stop no parity lt 0 5 at 1 absorbance 340 630 nm NADH solution 196 at 2 absorbance 340 630 nm NADH solution 3 at absorbance 340 630 nm NADH solution 0 5 at 1 absorbance 405 630 nm PNP solution 1 96 at 2 absorbance 405 630 nm PNP solution lt 3 at absorbance 405 630 nm PNP solution Better than 0 003 A hr monochromatic after warm up Better than 0 001 A hr bichromatic after warm up 90 seconds photometric 15 minutes for temperature compartment Z80 microprocessor 8 K bytes Static RAM SRAM 8 K bytes non volatile RAM NVRAM 115 230 VAC 50 60 Hz 10 60 VA 35 x 40 x 15 cm 10 Kg 10 cm c
18. S 232 signals in a non standard pinout The data format is 2400 baud 8 bits 1 stop bit and no parity The pinout for the serial port is GND TX RX DTR 9 NC Contact your distributor to obtain a serial printer cable Service Manual HUMALYZER 2000 Page 8 Error Messages Error messages are displayed when the instrument fails to operate correctly They are intended to help the operator locate the problem If error messages appear frequently a hardware problem is usually indicated The following error messages indicate possible interface or component problems Lamp Failure The lamp does not appear to illuminated at all Low voltages were detected for all filter positions See the section Photometer under Troubleshooting Lamp Output Low The lamp does not appear to be illuminated sufficiently Low voltages were detected for some filter positions This is possibly due to degraded filters See the section Photometer under Troubleshooting Printer Paper Jam The internal printer paper path is obstructed Clear the paper path If the paper path is clear Check the 14 pin DIP cable connecting the printer PCB to the interface PCB Printer Not Ready The external printer attached to the parallel port or serial port is out of paper or otherwise unable to print If chronic this may indicate an incorrectly wired external cable Waste is Full XX The waste material has reached the level sensors If the waste bo
19. Service Manual HUMALYZER 2000 INTRODUCTION 222222 3 PRINGIEPEES OEXOPERATIQU ee ee ee ee oneri dd 3 PHOTOMETER 3 TEMPERATURE CONTROL 3 FLOWCELL AND VACUUM PUMP Ne 4 DISPLAY KEYPAD AND THERMAL PRINTER 1 4 SYSTEM CONTROL 4 TROUBLESHOOTING una eis ae ee een ee 5 POWERSUBPEF Biete ee 5 PHOTOMETER ER ee 5 EEOWEEEE em NE REN 2 6 VALVE EER CSE SE EERE REED ERLE E 6 VACUUM PUMP AND PUMP CONTROL PCB 7 TEMPERATURE CONTROL re ee a 7 MAIN RO Barnoin ae esna P M 7 INTERNAL PRINTERS od een een ale 8 KEYPAD rer ea 8 DISPLAY E T 8 SERIAL POR Mc 8 ERROR MESSAGE sd 9 SERVICE PROCEDURES aan 11 OPENING THE losa SRR 11 CLEANING THE FEOWEEEL 12 FLOWCELL TUBING ee 13 FLOWCELL ADJUSTMENT ee 15 FEOWEEELBISASSEMBER taste 16 VALVE TUBING REPLACEMENT 17 VACUUM ABJUSTMENT aiiis oq eie Uo an 18 LAMP REPLACEMENT Rat 20 FIETEISREBEAGCEMEDBT st nln td id oat be 21 FILTER K
20. TIGI DO NOT CHANG APPLIED FOLLOW MAINS TRANSFORMER GROUNDED FRAME AND CHASSIS PART GT9725 ONLY NO SHIELD ON 06C SPLIT BOBBIN TYPE FLOWCELL SENSOR BOARD VALVE ASSY GT9725 or PRIMARY WINDINGS SECONDARY WINDINGS NE 264 A BLK WHT 5 VOLT PHOTOMETER ASSEMBLY 1 1 3 No 1 GRN DIGITAL CON 5 VOLTS No 2 GRN YE GRN BLU BLU MAIN PCB C No 3 ross i GRN Y POINTS GRN Hp PEN 5 pom 15 VDC peu ht t2 TYPE 2 LIMITED GRN O SECONDARIES 1 VAC BLU P TO 12 VDC AND 1 2 5 VDC Hl aed Ls 1 T PANEL SUBASSHMBLY Rev C PCB Layouts Main PCB 3015 wo JON 0 In EILO ons lee Wood ow SS3N3UVAY ec e CANTER PCO FAN 255529 909 4 nm WOE 184655 0 r3 58 7571 nn 9018138
21. ata the wavelengths for the two optional filters are stored in non volatile memory In the event this data is lost or corrupted the following message will be displayed and printed Filter Labels 788 Clrd You will need to re enter the filter labels for two of the filters Open the instrument and locate the filter label on the side of the photometer cover Key 7 is xxx Key 8 is xxx xxx is a three digit wavelength value Press MENU type in 248 and press ENTER You will be prompted Change Filter Names Key 7 nm Type in the wavelength for Key 7 that is printed on the label and press ENTER Repeat for Key 8 Press CLEAR twice to return to the main prompt Service Manual HUMALYZER 2000 Page 28 Temperature Calibration Materials and equipment 12 mm borosilicate tube Reference thermistor probe YSI Inc 44108 or equivalent 30K 25 0 2 interchangeability Humalyzer 2000 Temperature Calibration Fixture Digital ohmmeter 0 596 or better accuracy Ensure that the flowcell is active Press CELL Press YES to activate the flowcell Press CLEAR twice to revert to the temperature display Place a 12 mm borosilicate test tube filled with 1 ml of water into the incubation block Use a station near the center of the block Suspend the reference thermistor probe in the approximate center of the tube Remove the flowcell and install the Temperature Calibration Fixture into the read well Allow 15 minutes for
22. cribes the various screens and filters in that position Each element is separated by a slash The first 3 digit value in this column is always the wavelength of the interference filter in nanometers The other values indicate either dot screens neutral density filters or transmittance filters A D indicates a dot screen and the first two digits are the percent transmittance For example 90D indicates a 9096 dot screen A 3 digit value other than the first value in the line indicates a transmittance filter The first digit is the quantity the last two digits are the percent transmittance For example 115 indicates 1 15 transmittance filter A 2 digit value separated by a decimal point indicates a neutral density filter The value is the absolute absorbance of the filter BLOCKED indicates an opaque disk is installed in that position Manufacturer lot number LOT is the interference filter manufacturers production code The percent transmittance of the interference filter is also specified in this column The production date DATE is the date the filter wheel was assembled POS WAVELENGTH LOT DATE 1 340 3YGF 58 8 5 23 94 2 BLOCKED 5 23 94 3 BLOCKED 5 23 94 4 630 90D 115 170 4GDB 80 6 5 23 94 5 580 90D 115 170 3ZCJ 65 5 23 94 6 545 215 SWEF 74 4 5 23 94 7 505 215 15D AFLR 67 6 5 23 94 Service Manual HUMALYZER 2000 Page 23 8 405 115 900 4HDL 83 5 23 94 Service Manual HUMALYZER 2000 Page 24 Calibration
23. eight filters A removable flowcell provides extremely rapid fluid sampling with low carryover A built in vacuum pump and an external autoclavable waste bottle with level sensing are supplied standard When the flowcell is removed the instrument accepts standard 12 mm round tubes as well as 1 cm square cuvettes The instrument also contains an incubation block with 12 round tube stations Both the incubation block and the read well are temperature controlled to 37 C Principles of Operation Photometer The light from a tungsten halogen lamp is passed horizontally though the tube or flowcell and the sample it contains The sealed box on the opposite side of the read well contains a rotating filter wheel and photodiode The filter wheel contains eight interference filters of various wavelengths and is speed controlled to approximately 3 rps under software control As each filter passes in front of the photodetector an infrared optical switch triggers a sampling of the peak voltage produced by the photodetector The voltage is then fed to a comparator which compares the sampled voltage to the output of an exponential capacitor decay circuit The pulse at the output of the comparator enables a 16 bit counter in an 8254 programmable timer The photodetector output is proportional to the intensity of the light whereas the width of the positive phase of the comparator output is proportional to the absorbance The resistance across the log cap RC decay
24. equilibration Set the DMM to read 20K Read the resistance of the thermistor and the fixture If the resistance is between 17724 and 18712 no adjustments are needed Otherwise continue to the next step Calculate the temperature offsets using the following formula T R 18210 Where C R is the measured resistance C is equal to 750 if the measured resistance is greater 18210 C is equal to 720 if the measured resistance is less 18210 Select test 210 The display shows Block adjust Type the offset calculated above for the block and press ENTER The display shows Cell adjust Type the offset calculated for the cell and press ENTER The maximum allowed adjustment is 1 8 Entering values that exceed this net number will produce an out of range message Repeat steps 4 through 8 until the temperature is 37 C 0 3 Select test 213 to see the current net adjustments Record the new values on the calibration label located on the bottom of the instrument Service Manual HUMALYZER 2000 Page 29 Absorbance Calibration Materials and equipment 12 mm borosilicate tube Reference spectrophotometer Reference material which peaks at one of instruments wavelengths Precise 1 2 serial dilution of the reference material Ensure that the flowcell is active Press CELL Press YES to activate the flowcell Press CLEAR twice to revert to the temperature display Place a 12 mm borosilicate test tube filled with 1 m
25. group is discussed possible problems and solutions are outlined Power Supply The power supply consists of a transformer two diode bridges and three regulators Line voltage supplied to the transformer is converted to 36 VAC center tapped which feeds diode bridge BR2 The center tap is used as a reference for regulators and VRS which provide 15 VDC and 15 VDC for analog circuitry Another transformer output supplies 11VAC to diode bridge BR1 which provides 12VDC raw 11 13 VDC nominal to the system and VR3 regulates this down to 5VDC for the logic and other low voltage control circuits A dead instrument may be just a blown fuse However a blown fuse may indicate a problem on the main PCB or transformer Also the voltage select switch setting must match the line voltage If there is no voltage present at J6 check the fuse To replace the fuse disconnect the power cable and open the instrument Remove the two 4 screws holding the AC cover to the rear panel and lift the AC cover off towards the front of the instrument Locate the double clip fuse holder mounted on the inside of the rear panel Locate the blown fuse and remove it with a fuse puller or carefully pry it out with a small screwdriver Install a 0 6A 3AG slow blow fuse Use only the recommended fuse Do not substitute other rating Replace the AC cover If voltage is present at J6 check for input voltage on VR3 and VR5 If no voltage is present the di
26. he white ceramic print head Do not scratch the platen or pry with sharp objects This will destroy the printer Carefully remove any paper or debris with a pair of tweezers Turn off the power Switch wait 5 seconds then turn on the power switch The print head should move to the right side and then return to home position at the left An alternate method of removing a particularly stubborn wad of paper is to move the print head manually To do this remove the rear dress panel Look at the front of the printer mechanism Locate the small gear on the right side which turns the grooved steel print head shaft Turn the gear so that the print head moves away from paper wad and the paper can be easily removed Turn the power switch off and back on again as described above Keypad The keypad is a sealed membrane switch layer which is not serviceable You can test the keypad by pressing AUX then pressing all keys except CLEAR and ENTER Note that each key causes the instrument to beep and display a character Press CLEAR and the display clears Press ENTER to end the test Display The display is a 24 x 2 line super twisted nematic liquid crystal display module with integral controller The display should be clearly legible at all times with no dark spots or stray dots There are no adjustments Serial Port By using a special cable a serial printer or computer with a serial port may be connected This is a male 9 pin DB style connector using R
27. l of water into the read well Select test 186 and confirm that all of the filter voltages are between 2 00 and 11 00 volts Determine the absorbance of both the 1 1 reference material and the 1 2 dilution on a reference spectrophotometer The 1 2 dilution should be in the range of 8 to 1 2 absorbance The 1 1 material should be in the range of 1 6 to 2 4 absorbance Press ABS to place the instrument into the absorbance mode Select the required wavelengths Blank on the same diluent material used to prepare the concentrations Read the 1 2 dilution Divide the absorbance as read on the reference spectrophotometer by the reported absorbance value Exit the absorbance mode and select test 212 At the prompt Absorbance factor x xxx enter the ratio calculated When this factor is entered the instrument will display a net factor the new calibration data Press CLEAR twice and restart the absorbance mode Blank as before on the diluent The 1 2 dilution absorbance should now agree with the reference to within 005 absorbance Read the 1 1 concentration It should agree within 2 of the reference If necessary carefully adjust trimpot TP3 to achieve this agreement Re blank and reconfirm the values Select test 213 to print the new calibration data Enter these values on the calibration data label on the bottom of the instrument Service Manual HUMALYZER 2000 Page 30 Specifications Specification Date Model Name
28. learance on all sides Page 32 Replacement Parts See Figures 13 15 for illustrations NZ Description Lamp Fuse Printer paper Keypad domed Overlay with keypads Main PCB Interface PCB Display LCD Photometer assembly Incubation block assembly Cover assembly Fan Filter wheel belt NV RAM battery backed Printer mechanism Printer interface PCB Pump control PCB Valve assembly Waste bottle assembly 500 ml Internal cable set Vacuum pump Tubing kit Exhaust filter Cell insert Replacement filters 340 nm 405 nm 505 nm 545 nm 580 nm 630 nm Special filters Service Manual HUMALYZER 2000 Cat 18340 05 18340 10 18310 05 18380 05 18380 10 18350 05 18350 10 18340 25 18370 05 18340 35 18380 20 18340 30 18380 05 18340 15 18360 15 18350 15 18350 20 18360 10 18320 05 18340 20 18340 40 18320 10 18320 15 18370 10 18330 30 18330 05 18330 10 18330 15 18330 20 18330 25 Specify Page 33 Page 34 Service Manual HUMALYZER 2000
29. ls completely To check for low lamp output run test 186 Press MENU then type 186 and press ENTER The instrument prints the detected voltage for each filter The printout shows the wavelength position and the voltage for each filter The voltages should be between 2 and 10 volts If all or most of the voltages are low the lamp is the likely suspect As a final check measure the voltage at the lamp terminals If the lamp voltage is much lower than 4 5 VDC the regulator circuitry on the main PCB Q6 and VR1 may be the problem Otherwise replace the lamp Refer to the section Lamp Replacement The photometer PCB contains a photodiode a very sensitive op amp circuit and phototransistors Because the photodetector and related circuitry is solid state it should require no service Do not attempt to modify the photometer PCB The filter wheel turns on a shoulder screw shaft and is driven by the filter wheel motor via a neoprene belt The filter wheel itself requires no adjustments The optical interference filters contained in the wheel are of metal deposition type construction and are intended to be maintenance free for the life of the instrument However in a non ideal environment where extreme humidity or temperature are encountered optical characteristics on one or more filters may be compromised Run test 186 as described above If only one or two filter voltages are low the filter s may be suspect Refer to the service procedure Filte
30. m 9 0 150 8 50112 9 tf id GR i im dd e i to Gab t it op 2t 10 ER i 90 t Xd 27710 18 3 6 5 5 d 5 e 135181 lt 1100 YA I CM 905194 5 5 E 5 gt 8 90 5 4 t 25 1 od gin 1130 0002 4343 0003 2 010 gan eu E ER 151 001 1 2 Jatt 191001 190017 4055320590391 im en TT Y 894 0062 2 Page 42 Service Manual HUMALYZER 2000 urod 10 1 0 gt gt 24 saw mo JL r 4 209 WIE 2 SITE Y mg B 134 NUD 9 x 21 veoozuin us 38022 AST 012 14405 843 04 31883 08819 1001 3 330 YIH 113221 01 veoozn n 001 vcoozun a suan ya BaN 1ddns Y 330 onar 148 2 5 Rev 43 Service Manual HUMALYZER 2000 Rev C Main PCB 3 5 83d 031 3
31. nual HUMALYZER 2000 Rev C Parallel Buffer PCB 26 27 RAND 0 01uF EENE 2 I CNS Service Manual HUMALYZER 2000 Page 49 Rev C au nc 29 5 mt wos TOPINE 3 91 auIM L 9 Coo ooo X49 8 ec 82d NIVH PT 18391914 lt IVAWSHL Printer PCB Page 50 Service Manual HUMALYZER 2000 Rev C Incubation block PCB danv MOV MS 9 oos 9 46 OVI 40 621020 ZMS HOLMS 203125 39VLIDA MOL ASE SSSINUVH SNI3HIM OV OL 001 0061 804 Qat 39010 ANOAVLNSNOINOI i VOd 900566 MD 3 39018 NOUOV3H HOLOSNNOO 32018 NOLLOVSY SISSYHIOL SSWWNINY3L BAYH 977439198 51 Service Manual HUMALYZER 2000 Human Gesellschaft f r Biochemica und Diagnostica mbH Max Planck Ring 21 e D 65205 Wiesbaden Germany Telefon 49 6122 9988 0 Telefax 49 6122 9988 100 eMail human human de Internet http www human de 01 2004 04
32. ode bridge BR1 or BR2 may have failed Photometer The photometer assembly can be examined as several component systems Read well assembly and cell heat PCB Lamp and brackets Photometer PCB Filter wheel and motor The read well assembly serves to hold the tube or flowcell centered on the cell block between a fixed plate and a spring loaded plate Mechanical adjustments are not required The presence of a tube or flowcell is detected by an opto pair phototransistor and IR LED mounted at the ends of the cell heat PCB A stainless steel plunger in the bottom center of the cell block forces a small spring plate into the light path between the opto pair The lamp is tungsten halogen rated at 6V 10W and is intended to be maintenance free for the life of the instrument since the lamp is turned off automatically after 30 minutes of instrument inactivity However under heavy usage the lamp remains on for longer periods and may require replacement as the output becomes low near the end of its service life In addition a physical shock to the instrument may necessitate lamp replacement due to filament breakage The lamp and lens brackets simply hold the Service Manual HUMALYZER 2000 Page 5 lamp and lens in alignment with the rest of the assembly The brackets provide adjustments in both horizontal and vertical dimensions so that the filament can be centered on the light path The lamp must be replaced if output becomes low the lamp fai
33. ometer subassembly Locate the 3 6 screws holding photometer assembly to the chassis From the bottom of the instrument remove these screws and washer Refer to Figure 11 Remove the 3 6 32 screws retaining the photometer cover Set the photometer cover aside Remove the 3 4 40 screws securing the photometer PCB and gently move it aside Remove the filter wheel belt from the filter wheel and motor pulley Loosen the filter wheel shaft Remove the shaft and filter wheel Note the number of nylon washers and the order of installation Refer to Figure 12 Locate the filter to be removed Push the filter out of the wheel using the eraser end of a pencil or other soft blunt object Remove all filter components from that position in the wheel Locate the neutral density filters dot screen and transmittance filters that were included with the replacement filter and drop them first into the cavity Install the interference filter with the mirror side down Place 2 very small dabs of silicone adhesive on either side of the filler to prevent it from working loose Avoid smearing silicone adhesive on the filter surface Allow the adhesive to cure overnight Install the filter wheel taking care not to pinch washers under the shaft Reassemble the shaft and washers as disassembled Tighten the shaft securely The filter wheel should spin freely Install the filter wheel belt Be sure that the belt is centered on the filter wheel and
34. r Replacement Flowcell If proper care is taken to clean the flowcell between uses especially after highly proteinaceous liquids have been sampled the flowcell itself should require little or no maintenance The flowcell should be cleaned regularly as described in Cleaning the Flowcell If the flowcell tubing has become damaged or the flowcell is clogged the flowcell can be disassembled for cleaning and parts replacement Refer to the service procedures Flowcell Tubing Replacement and Flowcell Disassembly Erratic readings excessive dither may be a result of trapped air in the flowcell This can be caused by improper installation of or failure of the flowcell tubing or coupling tubes See the section Flowcell Tubing Replacement Check the insertion depth of the flowcell tubes into the coupling tubes Ensure that a leak free seal is made and that no tubes are kinked or pinched off Valve The valve uses a short length of silicone tubing If the valve operates but no sample is drawn up the pinch valve tubing may be blocked To check for clogged valve tubing unscrew the flowcell vacuum line from the Luer fitting Press PURGE and listen for aspiration If you hear aspiration the valve is operating but the flowcell is clogged If you do not hear aspiration the valve tubing is clogged See the section Valve Tubing Replacement Service Manual HUMALYZER 2000 Page 6 Vacuum Pump and Pump Control PCB The vacuum pump and pump con
35. red dot swaged end to the steel tube on the cell insert and feed the other end upward through the cell body Hint grasp the tubing with a small piece of 400 grit emery paper Do not kink the tubing Refer to Figure 5 C for the proper orientation Do not reverse the orientation as improper sampling will result Install the cell body and screws Feed the exit tube in through the rear of the flowcell Press the exit tube at least 7 mm to ensure leak free seal Figure 5 A Service Manual HUMALYZER 2000 Page 13 Figure5 B Figure 5 C Figure 5 D Service Manual HUMALYZER 2000 Page 14 Flowcell Adjustment The flowcell must be adjusted after replacing the flowcell tubing or any time the cell insert is removed or the adjustment set screw is disturbed 1 With the instrument on lift the flowcell out of the read well Do not unscrew the fitting Press MENU Type 189 and press ENTER The instrument will continuously report the detector voltage at 405 nm Record this value for reference in the next step Sample deionized water Visually confirm there are no air bubbles in the cell window Insert the flowcell into the read well until it bottoms out Note the value displayed If the displayed value is more than 50 greater than the value you recorded in step
36. rified with the use of NIST filters or by periodic comparison to a reference instrument that is known to be calibrated to NIST filters Calibration may be confirmed using a commercially available calibration check set which can be obtained from your distributor A periodic verification of instrument linearity is advised Since most lab test results are based upon standards rather than upon absolute absorbances the linearity of the instrument is the more critical indicator of instrument performance CAUTION DO NOT ALTER ANY POTENTIOMETER SETTINGS Changing these settings will make the factory calibration data invalid In the unlikely event the calibration data is lost or corrupted the absorbance factor is set to 1 000 and the temperature offset adjustments for the block and cell are set to 0 0 Do not enter values other than those recorded on the calibration label unless absolutely necessary Service Manual HUMALYZER 2000 Page 26 Restore Calibration Data Each unit is electronically calibrated at the factory The calibration values are entered by the keyboard and stored in non volatile memory Do ABS Set Test 212 Do Temp Set Test 210 If either of these messages are printed or displayed it indicates that the calibration values have been lost These messages will be printed each time that you turn on the instrument select a mode or recall a test The instrument will continue to operate but the calibration must be restored to ensure
37. rol PCB Turn the trimpot on the pump control PCB fully counterclockwise 3 Turn on the instrument Press PURGE and wait until the vacuum pump stops running The vacuum gauge should read much less than 18 cm Hg Slowly turn the trimpot clockwise until gauge reaches 17 8 cm Hg When turning the trimpot pause while the pump runs to maintain vacuum 4 Set the power switch to OFF O Disconnect gauge and tubing Reconnect the waste bottle Vacuum gauge Figure 9 18cm Hg From vacuum blue fitting of instrument To vacuum side of waste bottle Figure 9 B Service Manual HUMALYZER 2000 Page 18 Lamp replacement Replace and or adjust the lamp according the drawings below 1 Photometer RD oP Bees 9 2 Lamp removal mounting el pooo 4 Lamp adjustment 5 Spot alignment at entrance slot 0 Service Manual HUMALYZER 2000 Page 20 Filter Replacement Materials tools required Replacement filter set Phillips screw driver Flat blade screw driver Silicone adhesive Open the instrument as described in Opening the instrument Refer to Figure 3 Locate the photometer Unplug both ribbon cables from the main PCB This is an important step for relocating upon reassembly Mark with a pencil onto the chassis along the front and side edges of the phot
38. the bottle cap are used in conjunction with a detector circuit on the pump control PCB to indicate a full condition on the waste bottle Display Keypad and Thermal Printer A 24 x 2 line character Liquid Crystal Display LCD is mounted inside the cover Two 4 x 4 membrane switch keypads are sealed behind the front panel overlay The LCD connects to the interface PCB via a 14 pin DIP cable The tails of the keypad switch layers are fed through the cover and are connected to the interface PCB The printer assembly consisting of a 20 column thermal printer the printer PCB and metal supports is mounted to the rear dress panel and is connected to the interface PCB via a 14 pin DIP cable System Control The instrument is based on the 8 bit Z80 microprocessor The software is permanently stored in a 27256 EPROM A battery backed non volatile RAM random access memory chip MK48T08 incorporating a real time clock is used store data such as calibration test setups samples and also maintains the date and time A static RAM chip 6264 is used for temporary storage Almost all digital is accomplished with an 8255 Programmable Peripheral Interface Time intervals and pulse widths are measured using the counter channels of an 8254 programmable timer Service Manual HUMALYZER 2000 Page 4 Troubleshooting The instrument incorporates solid state electronics in a very reliable field proven design In this section each subassembly or component
39. trol PCB should require no maintenance If the vacuum pump runs continuously you probably have a leak in the vacuum side Check the waste bottle cap and fittings Check that the tubing is firmly seated on barbs and all fittings are tight Turn the fittings only until finger tight Do not over tighten the plastic Luer fittings If the waste bottle is located at a higher or lower level than the instrument such as on a shelf or if the instrument is used at very high altitudes the vacuum trimpot may require adjustment See the section Vacuum Adjustment If the vacuum pump is taking longer to achieve full vacuum runs much longer than usual but otherwise works the exhaust filter is likely clogged and should be replaced In the event that the exhaust filter gets wet due to a waste bottle spill it must be replaced See the section Exhaust Filter Replacement Temperature Control The temperature control system is very stable and ordinarily does not require adjustment or recalibration In the event calibration must be verified or re established refer to the section Temperature Calibration Main PCB Under normal circumstances there are no adjustments to be made to the main PCB Circuit failures are highly unlikely but if they occur it is recommended that the repairs be performed by factory authorized technicians There are ten test points on J8 which provide access to a number of vital signals The instrument can be observed in operation with
40. ttle is empty check the sensor cable jack and internal wiring for shorts On the pump control PCB check U2A Q2 and U3 Check the cable from the pump control PCB to the main PCB Empty Waste Press Enter See Waste is Full above Filter Wheel Err The instrument cannot correctly detect pulses from the filter wheel Check the photometer LED board and the phototransistors Q12 and Q13 on the photometer PCB See the section Photometer under Troubleshooting Vacuum Leak The self check routine was unable to achieve vacuum within the prescribed length of time Check for leaks at the waste bottle and at all connections Service Manual HUMALYZER 2000 Page 9 The following error messages indicate possible failure of the NV RAM U3 or the address decoding logic at U6 U7 and U16 U3 can be checked by substitution Error The checksum failed when a stored test was recalled The test was deleted Filter Labels 7 amp 8 The stored filter labels were corrupted or not found See the section Restore Filter Labels Water Values Reset The stored water absorbances were corrupted or not found Do Temp Test 210 The stored temperature calibration values were corrupted or not found See the section Restore Calibration Do ABS Test 212 The stored absorbance calibration values were corrupted or not found See the section Restore Calibration Service Manual HUMALYZER 2000 Page 10 Service Procedures
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