Part 2
Contents
1. A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED D a gaa tia Contact us 888 88 SOURCE sales artisantg com www artisantg com AMERSHAM BIOSCIENCES PhastSystem Development Unit User Manual 18 1060 96 Amersham Edition AB e KY Biosciences Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Important user information Reading this entire manual is recommended for full understanding of the use of this product The exclamation mark within an equilateral triangle is intended to alert the user to the presence of important operating and mainten2. The maintenance required by the operator concerns the gasket in the lid the gasket in the 10 port valve and the tubing between the 10 port valve and the chamber The gasket in the lid should be replaced when visibly damaged when filling and emptying takes longer than usual or when filling and emptying does not function properly the chamber must be air tight for filling and emptying The other two items should be replaced if they start to leak Replacing the lid gasket Just pull the gasket off Be sure to turn the recess in the new gasket outwards Replacing the 10 port valve gasket Opening the valve 1 Start the development run any method but press DEV pause continue as soon as EMPTYING P0 appears on the display When the 10 port valve is in position P0 the channel groove in the channel plate is always pointing to 12 o clock Disconnect the development unit mains power cable Raise the valve end of the unit about 30 Do not stand the unit on end or residual liquid may enter delicate parts in the unit 4 Unscrew the pressure plate taking a few turns at a time on each screw 5 Remove the distributor and distributing plate see figure Closing the valve 6 Without touching the surface of the distributing plate place the gasket against the smooth side with the notch in the gasket against the notch in distributing plate 7 Place the two parts into the distributor 8 Reassemble the valve Remem
3. 7 Polyvinylidine fluoride Polyvinyl chloride fluoro rubber Teflon PVC stainless steel EPDM PP concentration and alcohols and hydrocarbons moderate acids strong bases many solvents alcohols aldehydes most chemicals strong acids and bases in moderate concentration alcohols aldehydes and bleach most chemicals stroch acids and bases alcohols aldehydes and ketones strong acids in moderate con centration stroch bases in high concentration alcohols aldehydes and ketones hot stroch acids esters ketones and bleach extreme conditions hot acids ketones and hydrocarbons long exposure to salt solutions hot acids and aromatic hydrocarbons hot stroch acids aromatic hydrocarbons and bleach Ethylene propylene copolymer and terpolymer Polypropylene or polypropene Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 4 Installation 4 1 Unpacking 4 2 Mains connection 4 Installation Important The following information must be read to install your PhastSystem instruments correctly Unpack the equipment carefully and check the contents of the carton against the packing list Save the packing material and the carton in case PhastSystem must be returned Check the equipment for any visible signs of damage that may have occurred during shipment Removal of locking screw Remove the l
4. 88 SOURCE www artisantg com 1 Introduction 1 Introduction PhastSystem consists of a separation and control unit a development unit pre cast PhastGel separation media accessories and a technical support package These components work together to form a system for fast high resolution and reproducible electrophoresis PhastSystem development unit is controlled from PhastSystem separation and control unit The schematic diagram below illustrates the steps involved in producing a finished electrophoresis gel using PhastSystem with PhastGel separation media Flow diagram for PhastSystem Separation and control unit Place 1 or 2 gels on the separation bed Load PhastGel sample applicator s l Select a programmed separation method and press the start button when alarm sounds Vv 20 45 min remove the gel s y Total time 26 50 min Development unit 3min Place gel s in development chamber Select a programmed development method and press the start button when method stops 30 90 min l remove the gel s and analyze the results Specific detection via conventional methods e g Zymograms auto radiography blotting Total time 32 92 min The time intervals listed above will depend on the technique that is run Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE
5. end first and disconnect the tubing from the valve Remove the black cover plate for the tubing using a Philips screwdriver Shake the tubing to remove residual liquid Then remove the clamp at the chamber end Do not remove this clamp first residual liquid might then enter the unit Make sure that the new tubing rests in the recession when you put the cover back Cleaning Wipe the instrument regularly with a damp cloth Please be extra careful with the glass level sensor Let the instrument dry completely before use Servicing All service except the above described should be entrusted to qualified personnel only Please contact your local Amersham Biosciences representative for more service information 15 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 7 Ordering information and technical data 7 Ordering and technical information 7 1 Ordering 7 1 1 PhastSystem and accessories information Designation Quantity Code no Separation Control 18 1018 23 and Development Unit 120 V Separation Control and Development Unit 220 V 18 1018 24 Separation Controll Unit 120V 18 1200 00 Separation Controll Unit 220V 18 1200 10 Development Unit 120V 18 1200 20 Development Unit 220V 18 1200 30 PhastTransfer 18 1001 23 Includes gel backing remover Accessories PhastGel Sample Applicators 12 0 3 ul 50 18 1614 01 8 0 5 ul 50 18 1617 01 8 1
6. a list of ordering information for PhastGel media and chemicals Designation Quantity Code no PhastGel separation media PhastGel Gradient 4 15 10 17 0678 01 10 15 10 17 0540 01 8 25 10 17 0542 01 PhastGel Homogeneous 7 5 10 17 0622 01 12 5 10 17 0623 01 20 10 17 0624 01 PhastGel High density 10 17 0679 01 PhastGel SDS buffer strips 20 17 0516 01 PhastGel Native buffer strips 20 17 0517 01 PhastGel IEF IEF 3 9 10 17 0543 01 IEF 4 6 5 10 17 0544 01 IEF 5 8 10 17 0545 01 Dry IEF 10 17 0677 01 PhastGel Cassette 1 18 1001 01 PhastGel chemicals PhastGel Blue R 40 tablets 17 0518 01 PhastGel Silver Kit 1 17 0617 01 BandShift Kit 100 reactions 27 9100 01 Molecular weight calibration kits HMW SDS high molecular weight in SDS 10 17 0615 01 HMW High molecular weight 10 17 0445 01 LMW Low molecular weight 10 17 0446 01 PMW Peptide molecular weight 1 80 1129 83 pl calibration kits Broad pl kit pH 3 10 10 17 0471 01 Low pl kit pH 2 5 6 5 10 17 0472 01 High pl kit pH 5 10 5 10 17 0473 01 18 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 7 Ordering information and technical data 7 2 Technical information Capacity 7 2 1 Development unit Agitation Development Number of ports Temperature control range time error Development chamber material volume Temperature sensor Level sensor Gel holder Dimensions W
7. ance instructions in the literature accompanying the instrument Should you have any comments on this manual we will be pleased to receive them at Amersham Biosciences AB S 751 82 Uppsala Sweden Amersham Biosciences AB reserves the right to make changes in the specifications without prior notice Warranty and Liability Amersham Biosciences AB guarantees that the product delivered has been thoroughly tested to ensure that it meets its published specifications The warranty included in the conditions of delivery is valid only if the product has been installed and used according to the instructions supplied by Amersham Biosciences AB Amersham Biosciences AB shall in no event be liable for incidental or consequential damages including without limitation lost profits loss of income loss of business opportunities loss of use and other related exposures however caused arising from the faulty and incorrect use of the product Trade marks PhastSystem and PhastGel are the exclusive trade marks of Amersham Biosciences AB In view of the risk of trade mark degeneration it is respect fully suggested that authors wishing to use these designations refer to their trade mark status at least once in each article Copyright 1995 Amersham Biosciences AB All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted in any form or by any means without permission in writte
8. ber the channel groove in the channel plate should be pointing to 12 o clock The valve will not connect ports as programmed if the channel plate is turned by 180 9 Make sure the u shaped metal piece is sitting under the valve and that the hole for the screw is to the right Insert the screws in the pressure plate Screw in the left and right screws first a few turns then the bottom screw and finally the top screw Tighten the left and right screws securely and the top and bottom screws just until there is resistance 10 Re connect mains power cable Press DEV pause continue and then DEV start stop 13 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 6 Maintenance If the valve leaks open and check that all parts are correctly mounted Note If the valve still leaks you may have to replace the distributing and channel plates also Fixing plate Channel plate Distributing plate foe Pressure plate amp The 10 port valve Fig 6 The 10 port valve Start here The 10 port valve to chamber tube Fig 7 The 10 port valve to chamber tube 14 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 6 Maintenance Replacing the valve to chamber tubing Disconnect the power cable Let the unit rest on the end opposite to the 10 port valve Remove the clamp at the valve
9. e no 19 6005 02 The PhastSystem Development Unit is to be used exclusively with the PhastSystem Separation Unit Voltage selector communication cable on off Voltage selector Fig 3 PhastSystem controls rear Mains connection Plug the mains power cords into the input marked MAINS on the rear panel of the units Plug the cords into the wall outlet grounded to earth Important Always disconnect these cords when service the instruments Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 4 Installation 4 3 Turning the system on 4 4 Before use 10 The PhastSystem development unit is controlled from the PhastSystem separation and control unit The system is turned on by pressing in the on off button on the rear panel of the separation and control unit The development unit is automatically activated when a development method is started Temperature sensor A temperature sensor is placed on the underside of the lid in the development chamber enclosed in stainless steel The temperature sensor is calibrated before shipment but you may want to check and calibrate it before use See PhastSystem User Manual the chapter on Maintenance for instructions Before using the development unit we recommend that you run a cleaning method to remove dust accumulated during storage and shipment A cleaning method requires only distilled water and the level sensor s
10. eight 7 2 2 Common data Safety regulations safety class regulations Operating environment room temp humidity Electricity requirements mains voltage mains frequency power Power disturbance Mains failure 1 or 2 gels Gels are rotated in solutions during development Solutions are automatically pumped into and out of the development chamber 9 ports are available for solutions to enter and exit the chamber through port 0 is reserved for waste Up to 50 C the chamber only heats lt 4 min to heat solutions from 20 to 50 C 2 C from programmed temperature once the programmed temperature is reached Stainless steel Approximately 70 ml of solution will be pumped into the chamber Stainless steel Enclosed in glass Stainless steel 300 x 300 x 138 mm W x L x H 4 8 kg Class 1 apparatus Built according to UL 1262 and IEC 348 4 40 C Max 95 100 120 VAC 120 V model 220 230 240 VAC 220 V model 50 60 Hz Maximum 200 VA Development unit 130 VA Separation unit 330 VA Together The system IS protected against mains disturbances and static discharges For power failures lasting less than 5 10 seconds running methods will automatically continue when power is returned For power failures lasting more than 5 10 seconds running methods are set to pause an alarm sounds and a message appears on the display informing you about the power failure 19 Artisan Technology Group Quality Instru
11. f you plan to use other chemicals for example to clean the unit you should first check the resistance of the wetted parts to the chemical in question The chemical resistance of a polymer depends on many factors including the temperature and concentration of the solution the application a compound that swells may function well as a static seal yet fail in dynamic applications and the period of exposure Table 1 below is intended as a general guide for the chemical resistance of the wetted parts in the development unit If you are in doubt about the resistance of wetted parts to a certain chemical test the parts first order spare sparts for such tests see Ordering information chapter 7 In general you should avoid using ketones hot strong acids and organic hydrocarbons Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 3 Description of the system Table 1 A general guide for the chemical resistance of the wetted parts in the development unit Wetted parts Material of Generally Generally construction resistant to attacked by Distributor and PVDF strong acids and ketones esters and distributing plate PVDF bases in moderate hot acids Gasket 10 port valve Tubing 10 port to chamber Tubing bottles to 10 port valve Chamber gel holder and temp sensor Chamber lid gasket Chamber lid These parts are illustrated in Fig 1 2 6 and
12. hield instead of gels The level sensor shield is in the gel holder in the development chamber when you receive PhastSystem For instructions see PhastSystem Users Manual the section on cleaning method The level sensor shield must remain in the chamber when running methods without gels otherwise the chamber will not fill Warning The level sensor on the underside of the development chamber lid is enclosed in glass and is quite fragile Use extreme care when cleaning this sensor Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 5 Operation 5 1 Preparing the development unit 5 Operation The procedure for running development methods comprises four steps making up the solutions connecting the bottles to the correct ports with the PVC tubing inserting the gels and pressing the start key The rest is automatic You should always keep a fresh stock of solutions Filter solutions to keep the channels in the development unit clear and to avoid precipitation on the gel s We recommend that you label the bottles and the tubing use the yellow tubing markers to mark the tubes with their corresponding port number 1 Remove the caps on the cap set from the ports that you plan to use 2 Connect the ports 1 9 as required to the solution bottles with PVC tubing Connect port 0 to waste Use an empty bottle 4 Check for kinks in the tubing Make sure that tubing is
13. mentation Guaranteed 888 88 SOURCE www artisantg com Amersham e Biosciences Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Printed in Sweden by TK i Uppsala February 1996 A rtisan Artisan Technology Group is your source for quality TecmoogyGroup new and certified used pre owned equipment FAST SHIPPING AND SERVICE CENTER REPAIRS WE BUY USED EQUIPMENT DELIVERY Experienced engineers and technicians on staff Sell your excess underutilized and idle used equipment TENS OF THOUSANDS OF at our full service in house repair center We also offer credit for buy backs and trade ins IN STOCK ITEMS www artisantg com WeBuyEquipment 7 EQUIPMENT DEMOS HUNDREDS OF InstraV ea REMOTE INSPECTION LOOKING FOR MORE INFORMATION MANUFACTURERS Remotely inspect equipment before purchasing with Visit us on the web at www artisantg com 7 for more our interactive website at www instraview com information on price quotations drivers technical LEASING MONTHLY specifications manuals and documentation RENTALS ITAR CERTIFIED D a gaa tia Contact us 888 88 SOURCE sales artisantg com www artisantg com
14. n form from the company Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com Contents 1 Introduction csincescdepnndagntiwewda ten andes oo 2 Important Safety Information eee ee eee 3 2 1 Safety precautions ias grew erat ewrinne eects 3 3 Description of PhastSystem Development unit 4 Onl D seription ie sisene e oaa Ee o a AERE OAE 4 3 2 Chemical resistence 4 00 cise ia cinen pied eae ee oes 5 4 Installationer dsccsiediuciededanedesntdaetentetecnan 7 41 Unpacking eiie ias aves a i 4 7 4 2 Mains connection ssssssssssrrrrrrurrrre 7 4 3 Turning the systems On 0c eee eee 9 AA Before Use 2c oias bhp owas dap oetes eas eee te E AE 9 5 OPCtahOti ascccccianantudansiwiavyemeenauane tang ene 10 5 1 Preparing the development unit 10 6 Maintenant vi55 cnd lt sb acad hanno dhe han Cee item Owe LOE 12 7 Ordering and technical information 0065 15 7 1 Ordering information s ssassn sssr s rrsan 15 7 1 1 PhastSystem and accessories 005 15 742 opare POMS ere vedadees sae e ae ei HERE 16 7 1 3 PhastGel media and accessories 17 7 2 Technical information s ndekae Seba hated een 18 7 2 1 Development Ut puctenceriuenea eda rreren 18 7 2 2 Common Galas ows goh4 ikke ee sn ieee ess cde Be 18 Artisan Technology Group Quality Instrumentation Guaranteed 888
15. ocking screw on the left of the underside of the development unit The air pump is mounted on a rubber support and fixed with this screw during shipment Save the locking screw in case you should ever need to ship the unit Leaving the screw in place will make the unit noisier but will not affect the operation Voltage selector setting The instruments are available in two versions one for 220 230 240 V AC referred here to as the 220 V model and one for 100 120 V AC referred here to as the 120 V model electricity supplies Check that the instruments have the correct voltage and code number printed on their back panel Set the voltage selectors on the rear panel of the separation and control unit and development unit according to your local electricity supply 100 120 V or 220 230 240 V To do this e Check the voltage range of the mains electricity supply Set the voltage selector to the appropriate setting according to the table below Voltage range Voltage selector setting For 120 V model instruments 90 110 100 108 132 120 For 220 V model instruments 198 244 220 230 216 264 240 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 4 Installation Fuses Each unit has two fuses Check that the fuses are correctly installed and intact Connecting the units Connect the separation and control unit to the development unit with the communication cable cod
16. pment solutions to the development chamber The port labelled 0 is reserved for waste that is solutions only exit through this port The gel holder liquid level sensor and temperature sensor are mounted in the lid of the development chamber and protrude into the chamber when the lid is closed Inside the unit there is a pneumatic pump for filling and emptying the chamber a 10 port valve for the selection of ports and a 3 port valve for the selection of pump functions i e creating vacuum or pressure in the chamber The pneumatic pump is connected to an opening in the lid of the chamber A gasket in the lid makes the chamber airtight when the lid is closed By creating a vacuum in the chamber liquid is drawn in through a hole in the bottom of the chamber Similarly by creating excess pressure in the chamber liquid is pushed out through the same hole in the bottom Level sensor Temperature sensor Gel holder Fig 1 Development chamber Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 3 Description of the development unit PVC tubing ered Cap set Tube markers 10 Port valve Fig 2 10 port valve The parts that come into contact with development solutions in the 2 Chemical development unit are resistant to chemicals typically used in 3 Chemica Coomassie and silver staining for example acetic acid methanol resistance and silver staining solutions I
17. securely submerged in the solutions Important The chamber fills with approximately 70 ml of solution The bottles should be filled with at least 75 to 80 ml of solution to allow for the residual solution in the tubing 5 Open the lid of the development chamber by pressing on the right end of the red bar 6 Check that the chamber gasket on the lid is secure Inserting the gels 7 Remove one gel from the separation bed with a pair of forceps use the tab of the gel backing Be careful not to touch the gel surface with your fingers since fingerprints stain and cloud the protein bands 8 Slide the gel gel surface down into the upper position of the gel holder Remove the other gel and slide it gel surface up into the lower position of the gel holder Note If you are developing only one gel slide it into the lower position gel surface up 11 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 5 Operation YV fe A AVG fo ee ea Chamber gasket a Lower gel A holder position A 4 Fig 4 Inserting the gel into the gel holder 9 Close the lid and lock it by simultaneously pressing down on the top of the lid and pushing in the red bar Fig 5 Closing the development chamber lid 12 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 6 Maintenance 6 Maintenance
18. ul 50 18 1618 01 6 4 ul 50 18 0012 29 Titration curve 50 18 1657 01 Sample well stamp 1 18 0079 01 PhastTransfer filter paper 50x50 mm 200 18 1003 18 16 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 7 Ordering information and technical data 7 1 2 Spare parts This is a list of spare parts that might be required when following the maintenance outlined in Chapter 6 A complete spare parts list is contained in the service manual Designation Quantity Code no Development unit Gasket dev chamber 1 18 0048 01 Gasket 10 port valve 1 18 9482 01 Cap set 10 port valve 1 18 0072 01 Valve kit 10 port valve includes distributing plate and channel plate 1 18 1019 61 Distributor 10 port valve 1 18 0029 01 Tubing PVC 5 meters 1 19 0182 01 Tubing kit 10 port valve to chamber 1 18 0192 01 Level sensor shield 1 18 0206 01 Fuse 175 mA SB 120 V model 2 18 1627 01 Fuse 80 mA SB 220 V model 5 19 6236 01 Tube markers 0 9 5 of each 50 18 0180 01 Foot 4 18 2341 01 Fuse 1 6 A SB 120 V model 2 19 3087 01 Fuse 800 mA SB 220 V model 5 19 3085 01 Mains power cord 120 V 1 19 2447 01 Mains power cord 220 V 1 19 2448 01 Communication cable 1 19 6005 02 17 Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 7 Ordering information and technical data 7 1 3 PhastGel media and chemicals This is
19. www artisantg com 2 Important safety information 2 Important safety information 2 1 Safety In keeping with good laboratory practice we advise you to take the precautions following precautions when dealing with the instrument 1 Regularly check all insulation cables take care not to damage the units especially the separation compartment s lid Note For full safety it is important that the lid is not tampered with 2 Ensure that the mains cables are plugged into fully grounded mains outlets 3 Check on the rear panel at the instrument that the correct voltage is printed on the back and that the voltage selector setting is in accordance with your local electricity supply See section 4 2 Mains connection 4 Allow only authorised service representatives to service or work on the electrical circuitry of PhastSystem 5 Avoid spilling buffers or other conducting liquids onto the instrument Artisan Technology Group Quality Instrumentation Guaranteed 888 88 SOURCE www artisantg com 3 Description of the development unit 3 Description of PhastSystem Development unit The visible parts of the development unit are a stainless steel chamber with a heating foil a rotating gel holder for one or two gels a temperature and level sensor on the underside of the lid and ten ports through which the development chamber can be filled and emptied 3 1 Description Ports labelled 1 9 are used to connect develo
Download Pdf Manuals
Related Search