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Agilent 1100 Series Nano Pump

1. Ei E E O O 16 4 5 6 s1 J1 J2 J3 P2 P8 ae pi 2 7 Sy os P9 P14 C l oad omon No ooggooog P15 Ee P16 P17 pBoooljoog P20 P18 cease P19 Geawaece sana 2 OOO P21 P22 Figure22 Board Layout of the NPM Board 4 Remove the connector screws from the GPIB Remote and from the analog pressure output connector 5 Remove the board Place it on an ESD kit WARNING The RFI spring plate sitting on the board connectors is very sharp Be careful not to cut yourself when removing it from the old board and sliding it onto the new board 6 In most cases the RFI spring plate Radio Frequency Interference spring plat prevents radio emissions from the instrument to ambient remains on the interface connectors of the board Carefully remove the spring plate from the old board and slide onto the new board before installing the new board in the pump the RFI spring plate is NOT part of an exchange board 1100 Series Nano Pump Service M anual 135 3 Repairing the Pump 7 If you have to exchange
2. cc Solvent cabinet ow Vacuum oo s Pump J ae n Well plate sampler l I y Co Column compartment eee Detector DDD o ooo efo Control module a G u Figure1 Recommended Stack Configuration Front View 1100 Series Nano Pump Service M anual Installing the Pump 1 Remote cable Analog signal to recorder CAN Bus cable H AC power BD oooo00 Analog signal to GPIB or LAN recorder to LC Figure 2 Recommended Stack Config
3. L o d 88558h J 0 0 oS RGN SS WALE CAN bus cable _ ik i ao S Be A Eee fe 558553 gt See or Cal 833323 SG Autosampler Thermostat cable Analog signal to recorder AC power AC power Figure4 Recommended stack configuration rear view 1100 Series Nano Pump Service Manual 11 1 Installing the Pump Installing the Nano Pump Preparations Locate bench space Provide power connections Unpack the pump Parts required Pump Parts from accessory kit see Nano Pump Accessory Kit Content G2226 68705 on page 5 Power cord for other cables see text below and Cable Overview on page 174 ChemStattion and or Control module G1323A B WARNING When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Ensure the line po
4. Parts and Materials 4 Table 28 Cables Overview continued continued Type Description Part Number 3396 integrator 03396 60560 not possible with the well plate samplers General purpose spade Lugs G1351 81600 Auxiliary Agilent 1100 Series vacuum degasser 61322 61600 CAN Agilent 1100 module to module 0 5m Ig 5181 1516 cables Agilent 1100 module to module 1m Ig 5181 1519 Agilent 1100 module to control module G1323 81600 External Agilent 1100 Series interface board to general purpose G1103 61611 contacts GPIB Agilent 1100 module to ChemStation 1 m 10833A cable Agilent 1100 module to ChemStation 2 m 10833B RS 232 Agilent 1100 module to a computer 34398A cable This kit contains a 9 pin female to 9 pin female Null M odem printer cable and one adapter LAN cable Twisted pair cross over LAN cable 10 feet long 5183 4649 for point to point connection Category 5 UTP cable 8 m long G1530 61480 for hub connections 1100 Series Nano Pump Service Manual 175 4 Parts and Materials Analog Cables O One end of these cables provides a BNC connector to be connected to Agilent 1100 Series modules The other end depends on the instrument to which connection is being made Table 29 Agilent 1100 to 3390 2 3 Integrators Connector Pin Pin Signal Name 01040 60101 3390 2 3 Agilent 1100 1 Shield Ground 2 Not connected ay 3 Center Signal 6 s 4 Conne
5. B _OFF A HPLC System 2Nano Pump _ Settr 0 8 Sequence 0 7 bar Ripple 1 Method 1100 Series Nano Pump Service Manual 235 6 Control Module Screens for the Nano Pump Settings screens Settings 236 In the Settings screen you can change the pump parameters Here you can enter values for the column flow not ready limit In addition you have access to a different set of parameters available through the F1 5 navigation keys The F7 key Default resets the pump to default values F8 On Off opens a window to turn on the pump Changes must be acknowledged with F6 Done E iced o EA ulmin 4 bar 0 002 pimin 7 Timetable a J Pressure Bottle Fillin gs a Rinne E Use the m key for the context sensitive menu Reset will load the pump default parameters The Status command pulls up the module specific setup screen Dana MSAN y Column Flow _Not Ready Limit Dannu nouuy 4 Solvents 2 Channel Selection 1100 Series Nano Pump Service Manual Settings More Control Module Screens forthe Nano Pump 6 Line SECA CODE omina 0 00RF yea Optimize Automated Fast Reconditioning after Analysis Dannu nouny Flush Time EAIN min Recond Time min E Enable Immediate Fast Comp Change Select Solvents to choose an appropriate calibration curve for binary solvent mixtures Please note that the left mobile phase i
6. 1100 Series Nano Pump Service M anual Control Module Screens forthe Nano Pump 6 Screens available from the System view System view Use the Esc key to until you receive Views on the F5 key Choose System from the pull down menu This screen shows the last activities in the system Line EJ Loc MEG Inj I Time MT Idle Paga 4 Wed 16 49 8 d T Ti ACTUAL G 85 00 gt XB OFF Pump Temp Lamp Time WET ide Ready Fri 14 51 PUMP OFF TEMP OFF LAMP OFF Module Message Id Date Time Cap Pump Setpoint changed INFD OG 02 14 49 24 Cap Pump Setpoint changed N 06 02 14 48 36 Cap Pump Channel A volume limit exceeded EMF 06 02 14 47 18 Cap Pump Purge valve off Col Comp Calibration done SME 06 02 14 47 43h __Plo System Control Use the F1 key Control and select the capillary pump Pump Temp O Wed 16 19 PUMP OFF TEMP OFF _ Module Message uence modified Module added 05 03 16 14 44 Beal Setpoint changed NFI 05 03 16 11 51 a 2Autosampler Timetable changed NI 05 03 16 08 39 4ColComp Timetable changed 1100 Series Nano Pump Service M anual 245 6 Control Module Screens for the Nano Pump System configuration 246 In the capillary pump s Control screen you can turn On or Off F8 the pump or Reset F7 the pump Here you receive information about the not ready conditions if needed Pump Temp O ee 0 00 ide Ready E nReady PUMP OFF i
7. 1100 Series Nano Pump Service Manual 121 3 Repairing the Pump Exchanging the Pump Seals and Seal Wear in Procedure When required Seals leaking if indicated by the results of the leak test Check both pump heads individually Tools required 3 mm hexagonal key 4 mm hexagonal key 1 4 inch wrench Parts required Seals pack of 2 5063 6589 standard or 0905 1420 for normal phase application For the seal wear in procedure Restriction capillary 5022 2159 1 Disassemble the pump head assembly of the leaky pump head see Removing and Disassembling the Pump Head Assembly on page 120 122 1100 Series Nano Pump Service Manual Repairing the Pump 3 2 Using one of the plungers carefully remove the seal from the pump head be careful not to break the plunger Remove wear retainers if still present Plunger 3 Clean the pump chambers with lint free cloth Ensure all particulate matter is removed Best cleaning results will be achieved by removing all valves See pages 109 112 and the capillary Inject solvent into each chamber Chambers 4 Insert seals into the pump head and press firmly in position 5 Reassemble the pump head assembly see Reassembling the Pump Head Assembly on page 127 Reset the seal wear counter and liquimeter as described in the User Interface documentation 1100 Series Nano Pump Service Manual 1
8. Connector Pin Pin SignalName Active 01046 60202 HP 1090 Agilent 1100 TTL 1 1 White Digital ground NC 2 Brown Prepare run Low 8 4 3 Gray Start Low 7 7 4 Blue Shut down Low I 8 5 Pink Not connected i NC 6 Yellow Power on High 3 7 Red Ready High 5 Key 6 8 Green Stop Low NC 9 Black Start request Low Table 40 Agilent 1100 to General Purpose Connector Pin Pin SignalName Active 01046 60201 Universal Agilent 1100 TTL 1 White Digital ground A El 2 Brown Prepare run Low ae 3 Gray Start Low OU g e E 4 Blue Shut down Low m ga aoe 5 Pink Not connected 50 oo 6 Yellow Power on High O0 of 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 1100 Series Nano Pump Service Manual 183 4 Parts and Materials BCD Cables One end of these cables provides a 15 pin BCD connector to be connected to the Agilent 1100 Series modules The other end depends on the instrument to be connected to The BCD output for the well plate sampler does not work with the integrators 3392 3 6 Table 41 Agilent 1100 to 3392 3 Integrators Connector Pin Pin SignalName BCD Digit 18584 60510 3392 3 Agilent 1100 10 1 BCD5 20 11 2 BCD7 80 3 3 BCD6 40 9 4 BCD 4 10 7 5 BCD 0 1 5 6 BCD 3 8 12 7 BCD 2 4 6 Key 4 8 BCD1 2 1 9 Digital ground 2 15 5V Low 184 1100 Series Nano Pump Service Manual Parts and Materials 4 Table 42 Agilent 1100 to 3396
9. Plate 1 OOOO O OOO QVOOOU0 OOO0DOOO OOU OOOO OO DUG OOO WOO OOO oe Figure 45 BCD output for the well plates Processo r Board identificatio BCD register External contacts N N 12 RA filter Line driver 250mA g 3 z 4x Figure 46 Block Diagram BCD Board 216 BCD connector External contact 1100 Series Nano Pump Service M anual Introduction to the Nano Pump 5 LAN Board The HP JetDirect cards are network interface cards used in HP printers One board is required per Agilent 1100 stack It is recommended to add the LAN board to the detector with highest data rate The LAN board can only be used together with a main board version G13XX 66520 DAD M W D VW D Pump ALS or G13XX 66500 FLD RID and above an Agilent ChemStation software revision A 06 01 or above The following cards can be used with the Agilent 1100 modules Table 49 LAN Boards Agilent Order Number Supported networks J 4106A Ethernet 802 3 RJ 45 10Base T J 4105A Token Ring 802 5 DB9 RJ 45 10Base T J 4100A Fast Ethernet Ethernet 802 3 RJ 45 10 100Base TX BNC 10Base2 Minimum firmware of the J etDirect cards is A 05 05 Recommended Cables For point to point connection not using a network hub use a twisted pair cross over LAN cabl
10. 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions Evaluating the results The test results are evaluated automatically Two pressure plateaus are checked one at 20 bar and the other at 300 bar The appropriate current is measured A further criterion is the difference between the two plateaus The limits are as follows e lt 85 EMPV current for the 20 bar plateau e gt 15 EMPV current for the 300 bar plateau e gt 15 difference between both EMPV current values 1100 Series Nano Pump Service Manual 85 2 2 86 Troubleshooting and Test Functions Flow Sensor Accuracy Calibration Step 1 Step 2 Step 3 Step 4 Step 5 Step 6 Step 7 Step 8 Description The flow sensor accuracy calibration test is designed to calibrate the flow accuracy and to compensate the electronic offset of the nano flow sensor This test should be done if high flow accuracy is needed at flow rates lower than 500 nl min The calibration of the flow sensor is based in the linear relationship between the flow rate and pressure drop in a capillary The zero point is corrected and calibration factors are evaluated The test begins with the initialization of the pump After initialization the EMPYV is opening to release the pressure No primary flow is delivered After 2 minutes the flow sensor offset and pressure offset are measured The primary flow is set to 500 ul min for 1 minute and the pressure dr
11. 5 GND DSR 6 6 DSR RTS 7 a 7 RTS CTS 8 8 CTS RI p 9 RI DB9 DB9 DB9 DB9 Male Female Female Male Figure 47 RS 232 Cable 222 1100 Series Nano Pump Service M anual Setting the 8 bit Configuration Switch Introduction to the Nano Pump 5 The 8 bit configuration switch is located next to the GPIB connector Switch settings provide configuration parameters for GPIB address serial communication protocol and instrument specific initialization procedures Figure 48 8 bit Configuration Switch Table 54 8 bit Configuration Switch Mode Select 1 2 3 4 5 6 7 8 GPIB 0 0 GPIB Address RS 232C 0 1 Baudrate Data Parity Bits Reserved 1 0 Reserved TEST BOOT 1 iL RSVD SYS RSVD RSVD FC Switches 1 and 2 define which set of parameters for example for GPIB RS 232C and so on will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory 1100 Series Nano Pump Service Manual 223 5 224 Introduction to the Nano Pump In the non volatile memory the parameters are kept regardless of whether you turn the instrument off and on again They will be kept until the same set of parameters is subsequently changed and power is reset All other previously stored configuration settings will still remain in the non volatile memory In this way you can store more than one set of par
12. Corrective Action Degasser and pump channels A and or B not flushed sufficiently air in the channels Wrong solvent Purge the degasser and pump channels thoroughly with isopropanol under pressure use the restriction capillary Install isopropanol Purge the degasser and pump channels thoroughly 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 All plateaus negative 300 4 260 4 200 4 1504 104 T T T T T T 15 2 25 3 35 4 Potential Cause Corrective Action Loose or leaky fittings Leaky mixer if installed Loose pump head screws in channel A or B Leaking seal or scratched plunger in channel A2 or B2 Leaking outlet valve in channel A or B Leaky damper Ensure all fittings are tight or exchange capillary Tighten the mixer fittings and nuts Ensure the pump head screws in channels A and B are tight Exchange the pump seals in both channels Check the plungers for scratches Exchange if scratched Exchange the outlet valve Exchange damper 1100 Series Nano Pump Service M anual 79 2 80 Troubleshooting and Test Functions First plateau negative or unstable and at least one other plateau positive 300 4 260 4 1504 Potential Cause Corrective Action Leaking outlet valve in channel A Loose pump head screws in channel A Leaking seal or scratched plunger in channel A2 Cle
13. Press F6 Select to configure online signals maximum are 3 Additional signals can also be chromatograms or temperature signals from other modules Use the Right Left selection keys to switch between Available Signals and Selected Signals Use the F8 key Move or Enter to move available signals into the box for selected signals or vice versa Use F7 to Setup ranges for each individual highlighted signal Press F6 Done to activate the changed settings and to return to the Plot screen Selected Signals ii Col Comp Temp Left ap Pump Flow Col Comp Temp Right Cap Pump Pressure Cap Pump B Cap Pump Ripple Time Range min 1100 Series Nano Pump Service Manual 243 6 244 Control Module Screens for the Nano Pump Method screens On the Analysis screen use the F3 key Method to view the parameters in a method Use the F8 key Save As to save the method in the module s The PC Card key F2 is only active when a PCMCIA card is inserted in the control module The PCM CIA card is only recognized if inserted before last start up Cap Pump i 8 00 min Use F2 key PC Card to transfer a method from to a PCMCIA card Use th Right Left selection keys to switch between PC Card and Instrument window Use the Up Down selection keys to select the method Use the F7 F8 keys Copy or Enter to move available methods between PCMCIA card and Modules F6 Deletes a selected method Ao y
14. Use F1 Purge Task to access the pump s Purge Task After selecting a channel you can edit its Purge Time and Flow Rate Use F8 Purge to start stop the Purge Task as defined or define the settings for the next Purge Task Changes must be activated with F6 Done src 0 OOM Note g mmm e Time min Flow ul min On the System screen use the F2 key Configure and select the pump _Pump Temp Pee BEAT idie Ready Controller Controler 1 05 03 16 14 44 a ND 05 03 16 11 51 NFI 05 03 16 08 39 1100 Series Nano Pump Service M anual Control Module Screens forthe Nano Pump 6 This screen gives you information about installed options and allows you to configure your pump to the filter and mixer type that is actually installed in the pump Changes must be activated with F6 Done uua 0 00PM Neg Filter Volume aJ gpl Mixer Volume pl Flush Time file Recond Time min E Enable Immediate Fast Comp Change Danes y ee H Bottle Filings 9 1100 Series Nano Pump Service Manual 247 6 Control Module Screens for the Nano Pump Screens available from the Records screen Records screen Use the Esc key until you receive Views on the F5 key Choose System from the pull down menu Use the F4 key Records and select the pump Errors are reported either into the F2 System Log or F3 Error Log Records is Time BORT idie Module Product Serial Version
15. e Blocked active inlet valve e Defective pump drive assembly e Defective CSM board Suggested Actions V Exchange the active inlet valve V Exchange the pump drive assembly V Exchange the CSM board 1100 Series Nano Pump Service Manual 63 2 64 Troubleshooting and Test Functions Wait Time out When running certain tests in the diagnostics mode or other special applications the pump must wait for the plungers to reach a specific position or must wait for a certain pressure or flow to be reached Each action or state must be completed within the time out period otherwise the error message is generated Possible Reasons for a Wait Time out e Pressure not reached e Pump channel A did not reach the delivery phase e Pump channel B did not reach the delivery phase e Pump channel A did not reach the take in phase Pump channel B did not reach the take in phase e Solvent volume not delivered within the specified time Probable Causes e System still in purge mode e Leak at fittings EMPV active inlet valve outlet ball valve or plunger seals e Flow changed after starting test e Defective pump drive assembly Suggested Actions V Ensure that purge valve is closed V Exchange defective capillaries V Ensure pump components are seated correctly If there are still signs of a leak exchange the appropriate seal active inlet valve outlet ball valve plunger seal V Ensure correct operating condition
16. Notices Agilent Technologies Inc 2002 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws M anual Part Number 62226 90100 Edition Edition 07 02 Printed in Germany Agilent Technologies Deutschland GmbH Hewlett Packard Strasse 8 76337 Waldbronn Germany Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connec tion with the furnishing use or per formance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this doc
17. V Change to the nano flow sensor 1100 Series Nano Pump Service Manual 55 2 Troubleshooting and Test Functions Leak Sensor Missing Probable Causes e Disconnected or defective cable e Defective leak sensor Suggested Actions V Ensure the connection cable is seated correctly V Exchange the leak sensor 56 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Servo Restart Failed Servo Restart Failed Pump channel A B Servo Restart Failed Pump channel B The pump motor in the nano pump was unable to move into the correct position for restarting When the nano pump is switched on the first step is to switch on the C phase of the variable reluctance motor The rotor should move to one of the C positions The C position is required for the servo to be able to take control of the phase sequencing with the commutator If the rotor is unable to move or if the C position cannot be reached the error message is generated Probable Causes e Disconnected or defective cables e Mechanical blockage of the nano pump e Defective pump drive assembly e Defective CSM board Suggested Actions V Ensure the pump assembly cables are not damaged or dirty Make sure the cables are connected securely to the CSM board V Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly V Exchange the pump drive assembly V Exchange the CSM board
18. Figure 40 Electrical Connections to the Nano Pump WARNING To disconnect the nano pump from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off 1100 Series Nano Pump Service Manual 203 5 Introduction to the Nano Pump Instrument Layout The industrial design of the module incorporates several innovative features It uses Agilent s E PAC concept for the packaging of electronics and mechanical assemblies This concept is based upon the use of expanded polypropylene EPP layers foam plastic spacers in which the mechanical and electronic boards components of the module are placed This pack is then housed in a metal inner cabinet which is enclosed by a plastic external cabinet The advantages of this packaging technology are e virtual elimination of fixing screws bolts or ties reducing the number of components and increasing the speed of assembly disassembly e the plastic layers have air channels molded into them so that cooling air can be guided exactly to the required locations e the plastic layers help cushion the electronic and mechanical parts from physical shock and e the metal inner cabinet shields the internal electronics from electromagnetic interference and also helps to reduce or eliminate radio frequency emissions from the instrument itself 204 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 Early Maintenance
19. Normal Flow Settings With the F2 key Timetable you can list and edit the timetable for the pump Timetable Press F7 key Insert to add entries F6 key Delete to remove or Enter to edit selected highlighted entries 1100 Series Nano Pump Service Manual 239 6 240 Control Module Screens for the Nano Pump Settings Pressure Timetable EJ EA Time Module Setting 0 00 Cap Pump B 2 00 Cap Pump B Edit the different sections of each timetable line Use the pull down menus if available Use the F6 key Done to view the entered lines of the timetable or F7 Enter to acknowledge current settings Timetable EA PRA IE FLA 0 oo Fara Setting Composition 2 B amp fields change with setting selected gt Use the m key for the context sensitive menu It gives you additional tools for the timetable Daacks ae 11 Copy Line 12 Paste Line 3Print Timetable With the F3 key Pressure you can change the settings for the pressure limits 1100 Series Nano Pump Service Manual Settings Bottle Fillings Settings Runtimes Analysis Status Control Module Screens forthe Nano Pump 6 acy OMe CRUE CBee o OORRI Pressure Limits Lower Q __ bar Upper bar Actual Pressure 4 bar With the F4 key Bottle Fillings you can adjust the settings for the bottle fillings to their current state Select F8 Refill to refill All bottles or a Selected bottle t
20. Optional The interface board BCD external contacts see page 215 The interface board LAN see page 217 Nano pump main board NPM The board controls all information and activities of all assemblies within the nano pump The operator enters parameters changes modes and controls the nano pump through interfaces CAN GPIB or RS 232C connected to the user interfaces Figure 41 and Figure 42 show block diagrams of this board ASIC Application Specific Integrated Circuit The application specific integrated circuit ASIC includes all digital logic for the core processor functions and also for module specific functions Motor Drive There are two identical motor drives for the drive assemblies Each drive comprises motor control motor amplifier drive and current control Active Inlet Valve Drive There are two identical valve drives for the two active inlet valves Each drive comprises two amplifiers for the two contacts of valve solenoid Solvent Selection Valve Drive This drive consists of amplifiers for the solenoids of the solvent selection valve 1100 Series Nano Pump Service Manual 207 5 208 Introduction to the Nano Pump Electromagnetical Proportional Valve The EMPV consists of an electro magnetic solenoid driven by a PWM Pulse Width Modulator Pressure Converter This block comprises a filter and amplifier for the pressure sensor signal a multiplexer an A D converter and an offset corr
21. Possible Causes and Suggested Actions continued Possible Causes Suggested Actions Notes Dirty EM PV micro mode Perform the EM PV cleaning procedure followed only by the EM PV performance test Refer to the Nano Pump Service M anual EM PV Cleaning on page 91 One of a capillary after the Remove the blocked capillary clean it with EM PV is partially or Acetone completely blocked Change the capillary Any system component Replace the analytical column which is offering a changing Replace the filter frit in front upstream of the restriction to the pump EM PV The vacuum micro degasser Try another vacuum micro degasser or is off or has become experiment to determine the performance using defective different degasser channels If the mobile phase is very sensitive to gaseousness use the micro degasser continuous mode Basic performance problems Perform the pump Leak Test Refer to the Nano Pump Service in the pump Manual Leak Test on page 73 1100 Series Nano Pump Service Manual 97 2 Troubleshooting and Test Functions Poor peak shape The peak shape has taken on a fronting or tailing characteristic Table 10 Poor peak shape Possible Causes and Suggested Actions Possible Causes Suggested Actions Notes Column performance has Tryanew column deteriorated Poorly made capillary e Using a flashlight and absorbent tissue At very low flow rates a leak may connections causing excessive dead volume or leaks ina
22. Pressure Above Upper Limit 40 Pressure Below Lower Limit 41 Pressure Signal Missing 42 Valve Failed 43 M issing Pressure Reading 44 Pump Configuration 45 Valve Fuse 46 Inlet Valve Fuse 47 Temperature Outof Range 48 Temperature Limit Exceeded 49 M otor Drive Power 50 Encoder Missing 51 Inlet Valve Missing 52 Electro M agnetic Proportional Valve EM PV Missing 53 Flow sensor missing 54 Unsupported Flow Sensor 55 Leak Sensor Missing 56 Servo Restart Failed 57 Pump Head Missing 58 Index Limit 59 Index Adjustment 60 Index Missing 61 Stroke Length 62 1100 Series Nano Pump Service M anual Initialization Failed 63 Wait Time out 64 Testing your Nano Pump 65 Micro M ode Pressure Test 65 Normal M ode Pressure Test 68 Leak Test 73 EMPV Test 84 Evaluating the results 85 Flow Sensor Accuracy Calibration 86 EM PV Cleaning 91 Basic System Troubleshooting 92 System Pressure Abnormally Low 93 System Pressure Abnormally High 94 EM PV failed to initialize micro mode only 95 Unstable column flow and or system pressure 96 Poorpeakshape 98 Failure to produce peaks or abnormally small peaks after injection 99 Wandering Detector Baseline 100 User interface displays error messages for specific modules 101 3 Repairing the Pump Introduction 105 Simple Replacements 105 Exchanging Internal Parts 105 Cleaning the Nano Pump 106 Using the ESD Strap 106 Overview about the Nano Pump 107 Simple Repair Procedures 108 1100 Series Nan
23. floating Line primary oy 24V 45A voltage circuits M PATRI 5V switching p 5V 3A regulator sense linear gt 15 V 3 A voltage regulators jo 15V 3 A total power consumption on 36 V and 24 V must not exceed 107 watts Figure 49 Main Power Supply MPS Block diagram To disconnect the instrument from line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off 1100 Series Nano Pump Service M anual Introduction to the Nano Pump 5 No accessible hardware fuse is needed because the main power supply is safe against any short circuits or overload conditions on the output lines When overload conditions occur the power supply turns off all output voltages Turning the line power off and on again resets the power supply to normal operation if the cause of the overload condition has been removed An over temperature sensor in the main power supply is used to turn off output voltages if the temperature exceeds the acceptable limit for example if the cooling fan of the instrument fails To reset the main power supply to normal operating conditions turn the instrument off wait until it is approximately at ambient temperature and turn the instrument on again The following table gives the specifications of the main power supply Table 62 Main Power Supply Specifications Maximum power 300 VA 2
24. modules Its properties are e the complete communication capabilities GPIB CAN LAN and RS 232C e memory management e ability to update the firmware of the main system Main System Its properties are e the complete communication capabilities GPIB CAN LAN and RS 232C e memory management e ability to update the firmware of the resident system In addition the main system comprises the instrument functions that are divided into common functions like e run synchronization via APG remote e error handling e diagnostic functions and so on or module specific functions like e internal events such as motor control flow rates and so on e calculation of compensation values for variable strokes and pressures 1100 Series Nano Pump Service Manual 213 5 5 Introduction to the Nano Pump Firmware Updates Firmware updates can be done using your user interface e handheld control module with files from a PC card or e Agilent ChemStation with files from floppy disk The file naming conventions are xxxx vvv DLB where xxxx is the product number e g 2226 for the G2226A Nano Pump and vvv is the revision number for example 503 is revision 5 03 For instructions refer to your user interface Update of main system can be done in the resident system only Update of the resident system can be done in the main system only main FW update Resident System Main System resident FW upda
25. see Replacing the Top Cover and Foam on page 154 1100 Series Nano Pump Service M anual Repairing the Pump 3 Exchanging a Pump Drive Never start the pump when the pump head is removed This may damage the pump drive The nano pump has two pump drive assemblies The A drive is located on the left side and the B drive on the right side Frequency Error message M otor Drive Power Tools required Screw driver Pozidriv 1 Wrench 1 4inch 4 mm hexagonal key Parts required e Pump drive G1311 60001 exchange part number G1311 69001 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 131 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector Remove the flow sensor For this disconnect first the capillary coming from the EMPV the capillary on the injection device port 1 Remove the pump head For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the active inlet valve cable at the Z panel Unclip the mixing chamber from its holder Remove the damper and the Z panel For this disconnect first 1100 Series Nano Pump Service Manual 145 3 146 Repairing the Pump the mixing capillary the capillary going to the mixer the active inlet valve cab
26. see Specifications on page 257 How Does Compressibility Compensation Work The compressibility of the solvents in use will affect retention time stability when the back pressure in the system changes for example aging of column In order to minimize this effect the pump provides a compressibility compensation feature which optimizes the flow stability according to the solvent type The compressibility compensation is set to a default value for each pump head independently The compensation value for each pump head can be changed through the user interface Without a compressibility compensation the following will happen during a stroke of the first piston The pressure in the piston chamber increases and the volume in the chamber will be compressed depending on backpressure and solvent type The volume displaced into the system will be reduced by the compressed volume When a compressibility compensation value for a pump head is set the pump processor calculates a compensation volume that depends on the system pressure and the selected compressibility value This compensation volume is added to the delivery stroke of the first piston How Does Variable Stroke Volume Work Due to the compression of the pump chamber volume each piston stroke of the pump will generate a small pressure pulsation influencing the flow ripple of the pump The amplitude of the pressure pulsation is mainly dependent on the stroke volume and the compress
27. 1100 Series Nano Pump Service Manual 57 2 58 Troubleshooting and Test Functions Pump Head Missing Pump Head Missing Pump channel A B Pump Head Missing Pump channel B The pump head end stop in the nano pump was not found When the nano pump restarts the metering drive moves forward to the mechanical end stop Normally the end stop is reached within 20 seconds indicated by an increase in motor current If the end point is not found within 20 seconds the error message is generated Probable Causes e Pump head not installed correctly screws not secured or pump head not seated correctly e Broken plunger Suggested Actions V Install the pump head correctly Ensure nothing e g capillary is trapped between the pump head and body V Exchange the plunger 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Index Limit Index Limit Pump channel A B Index Limit Pump channel B The time required by the plunger to reach the encoder index position was too short nano pump During initialization the first plunger is moved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the index position is reached too fast the error message is generated Probable Causes e Irregular or sticking drive movement e Defective pump drive assembly Suggested Actions V Remove the pump head and examine the
28. 173 Cable Overview 174 Analog Cables 176 Remote Cables 179 BCD Cables 184 Auxiliary Cable 187 CAN Cable 188 External Contact Cable 189 RS 232 Cable Kit 190 LAN Cables 191 ot Agilent Technologies ta Parts and Materials 4 Nano Pump Main Assemblies eth Nano pump main assembly Figure 25 1100 Series Nano Pump Service M anual 158 Parts and Materials 4 Table 15 Nano pump main assembly Item Description Part Number 1 Nano pump main board NPM 62226 66530 Exchange NPM board 62226 69530 2 Power supply 0950 2528 3 Solvent selection valve connecting cable G1312 61602 4 Flow sensor 61376 60004 5 Leak pan pump 5041 8390 6 Solvent selection valve half of a complete valve G1312 60000 Solvent selection valve screw 5022 2112 7 Pump drive assembly 61311 60001 Exchange pump drive assembly G1311 69001 8 Pump head see page 163 G1311 60004 9 EM PV holding screw 0515 0850 10 EM PV complete assembly valve and solenoid G1361 60000 11 AIV connecting cable G1311 61601 12 Damping unit 79835 60005 13 Fan assembly 3160 1017 1100 Series Nano Pump Service M anual 159 4 Parts and Materials Solvent Cabinet and Bottle Head Assembly Table 16 Solvent cabinet and bottle head assemblies Item Description Part Number Solvent cabinet assembly includes items 6 7 8 5062 8581 1 Solvent tubing 5 m 5062 2483 2 Tube screw pack of 10 5063 6599 3 Ferrules with lock ring pack of
29. 180 to 480 ul system pressure dependent for flow rates up to 2 5 ml min default settings calculated volume 20 to 400 bar 5880 psi system pressure User selectable based on mobile phase compressibility 1 0 8 5 solvents with pH lt 2 3 should not contain acids which attack stainless steel Upper pH range is limited by fused silica capillaries Agilent ChemStation for LC For pressure monitoring 2 mV bar one output Controller area network CAN GPIB RS 232C APG Remote ready start stop and shut down signals LAN optional 1100 Series Nano Pump Service M anual Specifications 7 Table 63 Performance Specification Agilent 1100 Series Nano Pump continued Safety and maintenance Extensive diagnostics error detection and display through control GLP features Housing module and Agilent ChemStation leak detection safe leak handling leak output signal for shutdown of pumping system Low voltages in major maintenance areas Early maintenance feedback EM F for continuous tracking of instrument usage in terms of seal wear and volume of pumped mobile phase with user settable limits and feedback messages Electronic records of maintenance and errors All materials recyclable Table 64 Performance Specification Agilent 1100 Series Micro Degasser Type Maximum flow rate Number of channels Internal volume Internal design M aterial in contact with solvents Specification 5 ml min per channel
30. 244 Screens available from the System view 245 Screens available from the Records screen 248 Diagnostics and Tests 254 7 Specifications Performance Specifications 258 A Safety Information General 262 Operation 262 Safety Symbols 264 Lithium Batteries Information 265 Danish Information 265 Radio Interference 266 Testand Measurement 266 Sound Emission 267 Manufacturer s Declaration 267 Solvent Information 268 Solvents 268 1100 Series Nano Pump Service M anual xi xii Index Agilent Technologies on Internet 269 271 1100 Series Nano Pump Service M anual Agilent 1100 Series Nano Pump Service Manual 1 Installing the Pump Site Requirements 2 Unpacking the Nano Pump 5 Optimizing the Stack Configuration 7 Installing the Nano Pump 12 Get the System Ready forthe First Injection 17 oe Agilent Technologies 1 Installing the Pump Site Requirements A suitable environment is important to ensure optimum performance of the nano pump Power Consideration The nano pump power supply has wide ranging capability see Table 1 on page 3 It accepts any line voltage in the range described in the above mentioned table Consequently there is no voltage selector in the rear of the nano pump There are also no externally accessible fuses because automatic electronic fuses are implemented in the power supply To disconnect the nano pump from line unplug the power cord The power supply still uses some power e
31. 3 Board cover 5001 3772 Included in item 1 Figure 34 Sheet Metal Kit 170 1100 Series Nano Pump Service Manual Parts and Materials 4 Foam Parts Table 25 Foam Parts Item Description Part Number 1 Foam kit including upper and lower foam 61312 68702 2 Bushing for pump drive 1520 0404 3 Board guide 5041 8395 1 upper foam 1 lower foam Figure 35 Foam Parts 1100 Series Nano Pump Service M anual 171 4 Parts and Materials Nano Pump Accessory Kit G2226 68705 Table 26 Nano Pump Accessory Kit Content G2226 68705 Description Part Number Insert tool 01018 23702 SST Solvent inlet filter x4 01018 60025 Waste tube 2 m 0890 1760 SST replacement frit 0 5 um 5022 2185 Wrench open end 7 16 1 2 inch x 2 8710 0806 Wrench open end 1 4 5 16 inch x1 8710 0510 Wrench open end 14 mm x 1 8710 1924 Wrench open end 4 mm x 1 8710 1534 Hex key 2 5 mm 15 cm long straight handle x 1 8710 2412 Hex key 3 0 mm 12 cm long x 1 8710 2411 Hex key 4 0 mm 15 cm long T handle x 1 8710 2392 Torque adapter G1315 45003 CAN cable 1 m long 5181 1519 Purge valve assembly G1311 60009 Purge valve holder 61312 23200 Screw for the purge valve holder 0515 0175 FS to Inj valve cap 350 mm 25 um G1375 87322 FS to Inj valve cap 550 mm 25 um G1375 87323 Flow sensor accuracy calibration capillary 8000 mm 25 um G2226 67300 ESD wrist strap 9300 1408 172 1100 Series Nano Pump Servic
32. 3 Remove the plastic cap with the gold seal from the outlet ball valve 4 Using a pair of tweezers remove the sieve Check the gold seal It should be exchanged when strongly deformed Place the valve in an upright position insert the sieve into the recess and replace the gold seal with the cap M ake sure that the sieve cannot move and is away from the seal area of the gold seal 5 Place a new sieve into the recess of the outlet ball valve and replace the cap with the gold seal 6 Check that the new valve is assembled correctly and that the gold seal is present 1100 Series Nano Pump Service Manual Repairing the Pump 3 Valve body Sieve Gold seal Plastic cap Figure 16 Outlet Ball Valve Parts 7 Reinstall the outlet ball valve and tighten the valve 8 Reconnect the valve capillary Valve capillary Outlet ball valve Figure 17 Exchanging the Outlet Ball Valve 1100 Series Nano Pump Service Manual 113 3 Repairing the Pump Installing the M anual Purge Valve Frequency When performing a leak test for verifying pump tightness When using the nano pump permanently in normal mode Tools required Wrench 1 4 inch Wrench 14 mm Wrench 4mm Parts required Purge valve assy G1311 60009 Adapter G1312 23200 Screw 0515 0175 Washer 2190 0586 Installing the Purge Valve 1 a uu F amp F W N 114 Switch off the pump module Disconnect the capillary from the mixer to the filter at the filter inlet
33. 5m Ig 5181 1516 Agilent 1100 module to module 1m lg 5181 1519 Agilent 1100 module to control module 1323 81600 188 1100 Series Nano Pump Service M anual Parts and Materials 4 External Contact Cable 080 o o of 10 6 O Cafe So co lt 8 O One end of this cable provides a 15 pin plug to be connected to Agilent 1100 Series module s interface board The other end is for general purpose Table 45 Agilent 1100 Series Interface Board to general purposes Connector Color Pin Signal Name G1103 61611 Agilent 1100 White 1 EXT 1 Brown 2 EXT 1 Green 3 EXT 2 Yellow 4 EXT 2 Grey 5 EXT 3 Pink 6 EXT 3 Blue 7 EXT 4 Red 8 EXT 4 ao Black 9 Not connected Violet 10 Not connected Grey pink 11 Not connected Red blue 12 Not connected White green 13 Not connected Brown green 14 Not connected White yellow 156 Not connected 1100 Series Nano Pump Service Manual 189 4 Parts and Materials RS 232 Cable Kit 190 This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter Use the cable and adapter to connect Agilent Technologies instruments with 9 pin male RS 232 connectors to most PCs or printers Agilent 1100 module to PC Table 46 RS 232 Cable Kit 34398A Instrument DB9 DB9 Male Female OCOoOWd Ss FwPr 2 3 4 5 6 7 8 9 DB9 Female DB9 Male 1100 Series Nano Pump Service M anual Parts and Materials LAN Cables Recomme
34. Close purge valve The slope and plateau is evaluated automatically Evaluating the Results on page 71 describes the evaluation and interpretation of the pressure test results Running the test from the Control M odule 1 Place a bottle of LC grade isopropyl alcohol in the solvent cabinet and connect it to channel A2 Block the injection device port 6 with a blank nut 01080 83202 Positioning the blank nut on page 69 Set flow for channel A2 to 2500 ul min and flush the degasser channel for about 5 minutes 4 Set flow to 0 ul min Connect the signal cable to the analog output at the rear of the nano pump only if an integrator is used Press Execute to initialize the pressure test 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Once the test is started the nano pump increase the pressure up to approximately 390 bar Afterwards the flow stops and the pressure drop is monitored and displayed on the control module The control module displays a graphical representation of the pressure Evaluating the Results on page 71 describes the evaluation and interpretation of the pressure test results 7 When the test is finished slowly unscrew the blank nut Evaluating the Results The sum of all leaks between the pump and the blank nut will be indicated by a pressure drop of gt 2 bar minute at the plateau Note that small leaks may cause the test to fail but solvent may not be seen l
35. Integrators Connector Pin Pin SignalName BCD Digit 03396 60560 3392 3 Agilent 1100 1 1 BCD5 20 2 2 BCD7 80 Be J 3 3 BCD6 40 0 4 4 BCD 4 10 o 5 5 BCD 0 1 ie 6 6 BCD 3 8 7 7 BCD 2 4 8 8 BCD 1 2 9 9 Digital ground NC 15 5V Low 1100 Series Nano Pump Service Manual 185 4 Parts and Materials Table 43 Agilent 1100 to General Purpose Connector Wire Color Pin SignalName BCD Digit G1351 81600 Agilent 1100 Green 1 BCD 5 20 Violet 2 BCD 7 80 Blue 3 BCD 6 40 Z Yellow 4 BCD 4 10 SS Black 5 BCD 0 1 SS Orange 6 BCD 3 8 Red 7 BCD 2 4 Brown 8 BCD 1 2 Gray 9 Digital ground Gray Pink 10 BCD 11 100 Red Blue 11 BCD 10 200 White Green 12 BCD 9 400 Brown Green 13 BCD 8 800 14 n c 15 n c 186 1100 Series Nano Pump Service M anual Parts and Materials 4 Auxiliary Cable One end of this cable provides a modular plug to be connected to the Agilent 1100 Series vacuum degasser The other end is for general purpose Table 44 Agilent 1100 Series Degasser to general purposes Connector Color Pin Signal Name G1322 81600 Agilent 1100 White 1 Ground Brown 2 Pressure signal Green 3 ah ee Yellow 4 we Grey 5 DC 5VIN Pink 6 Vent 1100 Series Nano Pump Service Manual 187 4 Parts and Materials CAN Cable Both ends of this cable provide a modular plug to be connected to Agilent 1100 Series module s CAN bus connectors Agilent 1100 module to module 0
36. Low NC 9 Black Start request Low 13 15 Not connected Agilent 1100 to 3396 Series II 3395A Integrators Use the cable 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 1100 Series Nano Pump Service Manual 181 4 Parts and Materials Table 37 Agilent 1100 to 3396 Series III 3395B Integrators Connector Pin Pin SignalName Active 03396 61010 33XX Agilent 1100 TTL 9 1 White Digital ground OG NC 2 Brown Prepare run Low ae 15 3 3 Gray Start Low NC 4 Blue Shut down Low co NC 5 Pink Not connected is NC 6 Yellow Power on High O 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Table 38 Agilent 1100 to HP 1050 HP 1046A or Agilent 35900 A D Converters Connector Pin Pin SignalName Active 5061 3378 HP 1050 Agilent 1100 TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low am 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 182 1100 Series Nano Pump Service Manual Parts and Materials 4 Table 39 Agilent 1100 to HP 1090 LC HP 1040 DAD or Signal Distribution M odule
37. M anual Troubleshooting and Test Functions Inlet Valve Fuse Inlet Valve Fuse 0 Pump channel A Inlet Valve Fuse 1 Pump channel B One of the active inlet valves in the nano pump has drawn excessive current causing the inlet valve electronic fuse to open Probable Causes e Defective active inlet valve Defective connection cable front panel to CSM board e Defective CSM board Suggested Actions V Restart the nano pump If the error message appears again exchange the active inlet valve V Exchange the connection cable V Exchange the CSM board 1100 Series Nano Pump Service Manual 47 2 2 48 Troubleshooting and Test Functions Temperature Out of Range Temperature Out of Range 0 Pump channel A Temperature Out of Range 1 Pump channel B One of the temperature sensor readings in the motor drive circuit are out of range The values supplied to the ADC by the hybrid sensors must be between 0 5 V and 4 3 V If the values are outside this range the error message is generated Probable Causes e Defective CSM board Suggested Actions V Exchange the CSM board 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Temperature Limit Exceeded Temperature Limit Exceeded 0 Pump channel A Temperature Limit Exceeded 1 Pump channel B The temperature of one of the motor drive circuits is too high The processor continually monitors the temperature of the drive circuits on the CSM b
38. PCMCIA card RESnnnn DLB and press execute If you want to update the main firmware press F7 key Transfer to turn the module into the resident mode LED on module should blink yellow Records Mime Time MOM Idle Ready Select File RSYNC Status Idle You need to transfer the module into its alternate system before you can update its firmware Press Transter to pass control to the alternate system After the reboot press Execute to update the modules firmware 0 Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key Records to select the Generic module In this screen the resident firmware revision is shown Records lies o OORT Ready Module Product Serial Version On Time EMF Controller G1323B DE01704451 B 02 04 0d00 00h G1376A R DE01700107 A 04 01 Od00 00h Use the F5 key FW Update to enter the Update section Select the a file from the PCMCIA card 1376nnnn DLB and press execute When the update has finished press F7 key Transfer to return the module into the normal mode LED on module should stay yellow 1100 Series Nano Pump Service Manual 251 6 252 Control Module Screens for the Nano Pump Changing the serial number Records iii inte 0 00 Ready Execut lt gt Transfe Select File KE Status Idle l You need to transter the module into it s alternate system betore you can update it s tirmware P
39. Result Status Nano Pump EMPY Cleaning Procedure Expected total time approx Simin ooo o et E TestProcedwe ooo o o 1 Connect bottle with aqueous A1 and organic solvent B81 fo o o 2 Release system pressure o 3 Block EMPV outlet with blank nut 01080 83203 4 Opening EMPY o o o 5 Start flushing both channels together abot 25 min Jo oo ooo ey 6 Execute EMPY cleaning procedure 1100 Series Nano Pump Service Manual 91 2 Troubleshooting and Test Functions Basic System Troubleshooting 92 In this troubleshooting guide you will find a Possible Causes Suggested Actions approach to troubleshooting and correcting certain capillary LC system problems The problems are categorized by the following symptoms System Pressure Abnormally Low 93 System Pressure Abnormally High 94 EM PV failed to initialize micro mode only 95 Unstable column flow and or system pressure 96 Poorpeak shape 98 Failure to produce peaks or abnormally small peaks afterinjection 99 Wandering Detector Baseline 100 User interface displays error messages for specific modules 101 This troubleshooting guide deals with systemic problems of the capillary LC For detailed diagnostic troubleshooting and repair information on specific LC modules status indicators error messages diagnostic tests etc refer to the Reference M anual supplied with the specific LC module 1100 Series Nano Pump Service M anual Troubleshooting and T
40. Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Leak Sensor Short The leak sensor in the nano pump has failed short circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current increases above the upper limit the error message is generated Probable Causes e Defective leak sensor e Leak sensor incorrectly routed being pinched by a metal component Suggested Actions V Exchange the leak sensor 1100 Series Nano Pump Service Manual 33 2 34 Troubleshooting and Test Functions Compensation Sensor Open The ambient compensation sensor NTC on the CSM board in the nano pump has failed open circuit The resistance across the temperature compensation sensor NTC on the CSM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor increases above the upper limit the error message is generated Probable Causes e Defective CSM board Suggested Actions V Exchange the CSM board 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Compensation Sensor Short The ambient compensation sensor NTC on the CSM board in the nano pump has failed short circuit The resistance across the temperature comp
41. be replaced 6 Turn the nano pump off then on again The Records screen should display the correct serial number 7 Ifa ChemStation is also connected re boot the ChemStation now as well Entering the Serial Number using the ChemStation Module serial numbers are entered by typing specific commands into the command line at the bottom of the main user interface screen 1 To enter a module serial number type the following command into the command line print sendmodule S lpmp ser YYYYYYYYYY Where YYYYYYYYYY is the 10 character serial number of the nano pump The first two characters are letters which should be capitalized 1100 Series Nano Pump Service Manual 139 3 Repairing the Pump The reply line will respond with RA 0000 SER followed by the module serial number you just entered 2 Turn off the nano pump then on again Then re boot the ChemStation If the serial number you have just entered is different than the original module serial number you will be given the opportunity to edit the configure 1100 access screen during the re boot of the ChemStation 3 After boot up the serial number you have just entered can be seen under the Instrument menu of the main user interface screen The serial number of the nano pump can also be seen by typing the following command into the command line print sendmoduleS lpmp ser The reply line will give the serial number of the nano pump Replacing the Nano Pump s F
42. for example immediately after changing a setpoint or while a self test procedure is running An error condition is indicated when the status indicator is red An error condition indicates the nano pump has detected an internal problem which affects correct operation of the instrument Usually an error condition requires attention for example leak defective internal components An error condition always interrupts the analysis A flashing yellow status indicator indicates that the module is in its resident mode Call your local service provider for assistance upon observing this error condition A flashing red status indicator indicates a severe error during the startup procedure of the module Call your local service provider for assistance upon observing this error condition Error Messages Indication Error messages are displayed in the user interface when an electronic mechanical or hydraulic flow path failure occurs which requires attention before the analysis can be continued for example repair frit exchange or exchange of consumables required In the event of such a failure the red status indicator at the front of the module is switched on and an entry is written into the instrument logbook 24 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Error Messages Select the error message you are looking for in the list below Each of the error messages is described in detail on the in
43. for pressure signal output and e one optional interface board e LAN 1100 Series Nano Pump Service Manual 209 5 210 Introduction to the Nano Pump EM PV kK Drive lt M otor drive B Encoder B Pump lt DriveB lt ___ head B Motor B J J Control Current Motor TD ata control B control B address Active inlet F valveB Valve drive B SSVA SSVB l lt 4 valve drives ASIC Active inlet Valve drive A valve A Option lt D Current zi Data control A a Motor address 4 control A Control Pump Motor A i head A _ DriveA J Encoder A gt Motor drive A Figure 41 Block Diagram Pump Controller Board 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 0 Multiplexer j Filter amp gt A D converter amplifier lt Pressure Damper gt Offset Pressure converter correction M gt Analog output Integrator Amplifier amp ambient measurement a Leak sensor D Drive gt Fan Senso Flow Control signal Data address Diagnose signals digital lt Diagnose signals analog Temperature of hybrid gt Figure 42 Block Diagram Pump Controller Board 1100 Series Nano Pump Service Manual
44. nano pump at the main power switch Unplug the pump from main power Disconnect cables from the interface board connectors Loosen the screws Slide out the interface board from the nano pump Install the new interface board Secure screws u FP WwW N Reconnect the cables to the board connector BCD Interface board Figure 21_ Exchanging the Interface Board 1100 Series Nano Pump Service Manual 129 3 Repairing the Pump Exchanging Internal Parts The following procedures require opening the main cover of the nano pump Always ensure the nano pump is disconnected from the line power when the main cover is removed The security lever at the power input socket prevents that the pump cover is taken off when line power is still connected To disconnect the nano pump from line unplug the power cord The power supply still uses some power even if the switch on the front panel is turned off When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used The electronics of the nano pump will not allow operation of the nano pump when the top cover and the top foam are removed A safety light switch on the main board will inhibit the operation of the nano pump Always operate
45. odule firmware revision 5 0x In case the control module s display seems to be frozen hang up due to a communication problem on the CAN bus unplug the control module from the HPLC module and reconnect 232 1100 Series Nano Pump Service Manual Control Module Screens for the Nano Pump Screens available from the Analysis view Analysis view This is the wake up screen if the Agilent 1100 capillary pump is the only configured Agilent 1100 module It is used to enter the most common pump method parameters Wed 16 02 BM Xa Resdy MI Column Flow we B A 100 Settings Timetable The m key allows access to the context sensitive menus Date amp Time allows you to change time settings Print Screen gives access to the print screen 6 About tells you the current firmware revision and the serial of your control module Setup view leads you to analysis view configuration for additional Agilent 1100 modules Restart re boots the control module Analysis Wed 16 02 1 1 ce ACTUAL 296 Column Flow _ 0 0 poate me B 3 About Settings Timetable Channel 2Mode 3 Purge A 102 4Reset i 5 Status 34 B 102 amp Setup View f s amt _ 2 Restart 1100 Series Nano Pump Service Manual 233 6 234 Control Module Screens for the Nano Pump Setup View On Off In the Setup view modules can be added or removed
46. right away 3 Put a paper towel over the leak sensor in the pump leak tray 4 Disconnect the channel A solvent tube from the Al port of the pump solvent selection valve Liquid may drip from the disconnected solvent tube M ake sure to follow appropriate safety precautions 1100 Series Nano Pump Service Manual 17 1 Installing the Pump 5 Connect the end of the solvent tube to the syringe adapter Slowly draw a syringe volume 20m1 from the solvent tube Disconnect the solvent tube from the syringe adapter and reconnect the tube to the A1 port of the solvent selection valve Eject the syringe contents into an appropriate waste container Repeat steps 4 to 6 for the three remaining solvent channels When all 4 solvent channels are manually primed remove the paper towel from the pump leak tray Make sure that the pump leak sensor is dry before turning on the pump Purging the Pump 1 Make sure that the 1 8 inch plastic waste tube is tightly connected to the barbed waste fitting of the pump EMPV and routed to an appropriate waste container Turn on the LC System All system parameters should be set to default The degasser should also be turned on at this time Initialize the system Then access the pump controls and make sure the pump mode is set to Normal Access the pump Purge control Set up a purge table which will purge all channels for 5 minutes each at a flow of 2500 ul min Then start the purge When
47. seconds Plunger B2 delivers with a flow rate of 3 ul min for 30 seconds The slope should be horizontal or slightly positive A negative slope indicates a leak rate greater than 3 ul min Plunger A1 delivers 50 ul min for approximately 8 seconds Plunger A1 with a flow rate of 3 l min for 30 seconds The slope should be horizontal or slightly positive A negative slope indicates a leak rate greater than 3 ul min Plunger B1 delivers 50 ul min for approximately 7 seconds Plunger B1 delivers with a flow rate of 3 ul min for approximately 30 seconds The slope should be horizontal or slightly positive A negative slope indicates a leak rate greater than 3 ul min At the end of the fourth plateau the test is finished and the nano pump switches off Running the Leak Test Tools required Wrench 1 4 inch Parts and materials Restriction Capillary G1313 87305 required Blank nut 01080 83202 Isopropanol 500ml 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 M ake absolutely sure that all parts of the flow path that are part of the test are very thoroughly flushed with IPA before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail This test requires the manual purge valve installed and the Electronic Flow Control EM PV and flow sensor bypassed To install the manual purge valve see Installing the M anual Pur
48. the nano pump with the top foam and top covers in place Internal components may be sensitive to electrostatic discharge ESD Always use an ESD kit when handling internal parts 130 The procedures in this section describe how to exchange internal parts You must remove the nano pump from the stack in order to open the main cover 1100 Series Nano Pump Service M anual Repairing the Pump 3 Removing the Top Cover and Foam Tools required Screwdriver Pozidriv 1 Preparations for this Switch off nano pump at the main power switch procedure Disconnect the solvent inlet tubes from the solvent selection valve or the adapter at the active inlet valve Beware of leaking solvents due to hydrostatic flow Remove leak funnel with the waste tube Remove the solvent cabinet from the nano pump 1 Remove the front cover by pressing the clip fastener 2Unplug the power cord and move the lever towards on both sides of the cover the power socket Clip fastener 1 1100 Series Nano Pump Service Manual 131 3 Repairing the Pump 3 Lift the clips on both sides of the top cover 1 Remove the top cover 2 4 Unscrew the screws on the top plate and remove the plate by lifting its back first and then sliding to the front 5 If an optional interface board is installed remove it from the nano pump switch 132 1100 Series Nano Pump Service M anual Repairing the Pump
49. the pump has been turned off for a certain time for example overnight oxygen will re diffuse into the channels between the degasser and the pump It is suggested to purge each channel at 2500 ul min for 1 minute at the beginning of each day Table 4 Choice of Priming Solvents for Different Purposes Activity Solvent Comments After an installation Isopropanol Best solvent to flush air out of the system After an installation second choice Ethanol or M ethanol Alternative to lsopropanol if no Isopropanol is available When switching between reverse phase Isopropanol Best solvent to flush air out of the system and normal phase both times 18 1100 Series Nano Pump Service Manual Installing the Pump 1 Table 4 Choice of Priming Solvents for Different Purposes continued Activity Solvent Comments To clean the system when using buffers Bidistilled water Best solvent to re dissolve salts After a solvent change Bidistilled water Best solvent to re dissolve salts After the installation of normal phase seals Hexane Good wetting properties P N 0905 1420 5 lsopropanol To clean the capillaries Acetone Best solvent to remove impurities from the capillaries 1100 Series Nano Pump Service Manual 19 1 Installing the Pump 20 1100 Series Nano Pump Service Manual Agilent 1100 Series Nano Pump Service Manual o 2 Troubleshooting and Test Functions e Status Indicators 23 ErrorMessages 25 Testing your N
50. to confirm the leak Potential Cause Autosampler Corrective Action Loose or leaky fitting Tighten or exchange the fitting or capillary Needle seat Exchange the needle seat Rotor seal injection valve Exchange the rotor seal Damaged metering seal or plunger Exchange the metering seal Check the plunger for scratches Exchange the plunger if required 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Leak Test The leak test is a built in troubleshooting test designed to demonstrate the leak tightness of the nano pump The test should be used when problems with the nano pump are suspected The test involves monitoring the pressure profile as the nano pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness and operation of the nano pump components Description The pump outlet is blocked with a blank nut and then the test is run using isopropyl alcohol IPA while monitoring the pressure profile using an integrator on the analog output or in the plot screen in the Control Module or the ChemStation The pressure profile is shown in Figure 9 Pump A Pump B Pressure sa Pump A Pump B Piston 1 Piston 1 Piston 2 Plateau4 bar Piston 2 Plateau 2 Plateau 3 pil Ramp 3 Plateau 1 E Ramp 5 Ramp 6 2504 200 5 Ramp 2 1504 100 4 50 4 Ramp 1 T T T T T T T T a 0 5 1 16 2 26 3 3 6 4 Time minutes Fi
51. use the PEEK blank nut provided in the accessory kit Don t connect a SST blank nut to the flow sensor outlet this could damage the flow sensor 1100 Series Nano Pump Service Manual 65 2 Troubleshooting and Test Functions Micro Mode Pressure Test OF x Start Explain Close Result Status Nano Pump PressweTest o Expected total time approx 10min o ee ae ee Test Procedure ee d O Connect bottle with isoprop 622264 A 2 P 2 Opening EMP o o o E 3 Start flushing channel for about 2min 4 Closing EMV o d o E 5 Disconnect capilary atflow sensor ouet B Put beaker underflow sensor outlet o 7 Flushing flow sensor 200u Imin PP 8 Release system pressure PT 9 Reconnect capilay at flow sensor outlet 10 Block system with blanknut_ PP 11 Execute EMPV cleaning procedure 12 ClsingEMPY o d o E 13 Running pressure test about 45min ooo o 14 Opening EMPV to release presse 15 Evaluating pressuretest PP Micro M ode Pressure Test Results The test results are evaluated automatically The sum of all leaks within the column flow path from the EMPV to the blank nut must be lower than100 nl min NOTE Small leaks with no visible leaks in the flow path can cause the test to fail 66 1100 Series Nano Pump Service M anual If the pressure test fails Troubleshooting and Test Functions 2 Ensure all fittings between the pump and the blank nut are tight and repeat the pre
52. wear counters display a value derived from pressure and flow both contribute to seal wear The values increment with pump usage until the counters are reset after seal maintenance Both seal wear counters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed Using the EMF Counters The user settable EMF limits for the EMF counters enable the early maintenance feedback to be adapted to specific user requirements The wear of pump components is dependent on the analytical conditions therefore the definition of the maximum limits need to be determined based on the specific operating conditions of the instrument Setting the EM F Limits The setting of the EMF limits must be optimized over one or two maintenance cycles Initially no EMF limit should be set When performance indicates maintenance is necessary take note of the values displayed by liquimeters and seal wear counters Enter these values or values slightly less than the displayed values as EMF limits and then reset the EMF counters to zero The next time the EMF counters exceed the new EMF limits the EMF flag will be displayed providing a reminder that maintenance needs to be scheduled 1100 Series Nano Pump Service M anual Introduction to the Nano Pump 5 The Electronics The electronics are comprised of four main components The nano pump main board NPM see page 207 e The power supply see page 228
53. 0 Series Nano Pump Service Manual 195 5 Introduction to the Nano Pump Each channel consists of an identical independently controlled pump unit Each pump unit includes a pump metering drive assembly and pump head assembly The pamphlet assemblies both consist of two identical chambers pistons and seals plus an active inlet valve and an outlet ball valve The channel flow outputs are initially joined by a low volume pre mixer and are then connected by a capillary coil to a pressure pulse damper The pressure pulse damper also serves as a pressure transducer which sends system pressure information to the user interface This output flow called primary flow is connected to the Electronic Flow Control EFC system The EFC system consists of an Electro Magnetic Proportioning Valve EMPV in series with a Flow Sensor The EMPV is protected from particles in the mobile phase by a solvent filter frit Responding to user entered column flow setpoint the EFC system determines how much of the main flow volume is ultimately delivered to the column The remaining main flow volume which is not required by the column is diverted to waste by the EMPV Under user control the EMPV can also function as a purge valve for purposes of solvent changeover etc In this case the EMPV is totally open and the total main flow is diverted to waste 196 1100 Series Nano Pump Service M anual Introduction to the Nano Pump 5 Degasser Fr
54. 00 W Continuous output Line Input 100 240 volts AC Wide ranging 10 line frequency of 50 60 Hz Output 1 24V 4 5 A maximum total power consumption of 24V and 36 V must not exceed 107 W Output 2 36V 2 5A maximum Output 3 5V 3A Output 4 15V 03A Output 5 15V 0 3A 1100 Series Nano Pump Service M anual 229 5 Introduction to the Nano Pump 230 1100 Series Nano Pump Service M anual Agilent 1100 Series Nano Pump Service Manual 6 Control M odule Screens for the Nano Pump Major keys on the Agilent 1100 Control Module 232 Screens available from the Analysis view 233 Screens available from the System view 245 Screens available from the Records screen 248 Diagnostics andTests 254 ott Agilent Technologies 231 6 Control Module Screens for the Nano Pump Major keys on the Agilent 1100 Control Module ESC Return to previous screen abort any change of parameters and toggle between the last two top layer views m Open context sensitive menus i Information Help Enter Store changed parameters or execute a choice Done If available Activate settings of current screen On Off Switch on individual Instrument s or complete System Start Start a location range or sequence Plot View online signals Views Change between analysis samples status system views The screens shown on the next pages are based on the following firmware revisions Control M odule firmware revision B 02 0x G1323B HPLC M
55. 10 5063 6598 4 Bottle amber 9301 1450 Bottle transparent 9301 1420 5 Solvent inlet filter SST 01018 60025 6 Leak pan solvent cabinet 5042 1307 7 Front panel solvent cabinet 5062 8580 8 Name plate Agilent 1100 5042 1381 Bottle head assembly includes items 1 2 3 5 G1311 60003 The assy comes with a glass solvent inlet filter Remove and replace it with the SST solvent inlet frit 01018 60025 Figure 26 Solvent cabinet and bottle head assembly 160 1100 Series Nano Pump Service Manual Parts and Materials 4 Nano Pump Hydraulic Path Table 17 Nano pump hydraulic path Item Description Part Number 1 EM PV to FS cap 220 mm 25 um for nanoflow sensor 61375 87321 2 Outlet ball valve to piston 2 capillary 61312 67300 3 FS to inj valve cap 350 mm 25 um for nanoflow sensor G1375 87322 FS to inj valve cap 550 mm 25 um for nanoflow sensor G1375 87323 4 Mixing capillary 61312 67302 5 Connection tube G1311 67304 6 Restriction capillary 61312 67304 7 Filter assembly includes frit 5064 8273 Frit 5022 2185 8 Filter to EM PV cap 280 mm 170 um G1375 87400 9 Damper to filter capillary 130 mm 250 um 01090 87308 10 Solvent tube pack of 4 61322 67300 Corrugated waste tube 120 cm re order 5 m 5062 2463 1100 Series Nano Pump Service Manual 161 A Q fa l LO Or AVR oe a 1100 Series Nano Pump Service Manual Parts and Materials 4 Figure 27 Nano pump hydraulic path
56. 162 Parts and Materials 4 Pump Head Assembly Table 18 Pump Head Assembly Item Description Part Number Pumphead assembly included items marked with G1311 60004 1 Outlet ball valve G1312 60012 2 Screw lock 5042 1303 3 Screw M5 60 mm 0515 2118 4 Apdater 61312 23201 5 Pump chamber housing G1311 25200 6 Active inlet valve complete with cartridge G1312 60010 Replacement cartridge for active inlet valve 5062 8562 7 Seal pack of 2 5063 6589 Seal pack of 2 for normal phase applications 0905 1420 8 Plunger housing including springs G1311 60002 9 Sapphire plunger 5063 6586 10 Support ring 5001 3739 11 Outlet valve to piston 2 capillary G1312 67300 1100 Series Nano Pump Service M anual 163 4 Parts and Materials Figure 28 Pump Head Assembly 164 1100 Series Nano Pump Service Manual Parts and Materials 4 Electro Magnetic Proportional Valve EM PV Table 19 EMPV Assembly Item Description Part Number 1 EM PV complete assembly G1361 60000 2 Filter to EM PV capillary G1375 87400 3 EM PV to flow sensor capillary nanoflow sensor 61375 87321 4 Screw 0515 0850 ov amp F Figure 29 EMPV Assembly 1100 Series Nano Pump Service Manual 165 4 Parts and Materials Flow Sensor Assembly Table 20 Flow Sensor Assembly Item Description Part Number 1 Nanoflow sensor assembly G1376 60004 EM PV to flow sensor capillary nanoflow sensor 61375 87
57. 211 5 Introduction to the Nano Pump 212 Inlet valve 4 24V Electronic AIVB Z panel drive B nice 24V motor B LT a 15V Hard 36V encoder B rive a ardware switch P 24V tor A pai Motor r i rocessor encoder A drive A tv Inlet valve 36V Electronic gt Hardware lt ANA lt Zpanel E drive A 24V fuse oo switch 24V SSV valve 36V Electronic SSV lt Z panel drive B ern f se SSV valve 36V Electronic driveA 424V fuse EMPV Leak Leak 15V D converter E sensor 24V Fan drive e Fan E Damper 15v Damper converter Analog Flow 36V lt _ sensor CSM ees power Power 15V lt supply supply 5V Figure 43 Interconnection Diagram NPM Board 1100 Series Nano Pump Service Manual Introduction to the Nano Pump Firmware Description The firmware of the instrument consists of two independent sections e anon instrument specific section called resident system e an instrument specific section called main system Resident System This resident section of the firmware is identical for all Agilent 1100 series
58. 23 3 Repairing the Pump Seal Wear in Procedure This procedure is required for standard seals only 5063 6589 but it will definitely damage the normal phase application seals 0905 1420 124 Fill a bottle with 100 ml Isopropanol and place the bottle head assembly of the pump head that is supposed to be worn in into the bottle Screw the adapter 0100 1847 to the AIV and connect the inlet tube from the bottle head directly to it Connect the restriction capillary 5022 2159 to the outlet of the EMPV Insert its other end into a waste container Turn the system in purge mode and purge the system for 2 minutes with isopropanol at a flow rate of 2 ml min Turn the system to standard mode set the flow to a rate adequate to achieve a pressure of 350 bar Pump 15 minutes at this pressure to wear in the seals The pressure can be monitored at your analog output signal with the handheld controller Chemstation or any other controlling device connected to your pump Turn OFF the pump slowly disconnect the restriction capillary from the EMPV to release the pressure from the system Reconnect the capillary going to the flow sensor and the connecting tube from solvent selection valve to the AIV Rinse your system with the solvent used for your next application 1100 Series Nano Pump Service M anual Exchanging the Plungers Repairing the Pump 3 When required When scratched Tools required 3 mm hexagonal key 4 mm h
59. 3 Do not connect a power plug to the nano pump module after removing the top covers A Safety light switch on the main board will prevent operation when the covers are removed The next figure shows the position of the light switch on the board 7 Position of the safety switch on the main board Light switch Foam lever 1100 Series Nano Pump Service Manual 133 3 134 Repairing the Pump Exchanging the Nano Pump Main Board NPM Board Frequency Tools required Parts required Board defective Wrench 14mm Wrench 7mm Wrench 5mm NPM board G2226 66530 exchange part number G2226 69530 1 Turn off the pump disconnect all cables and remove the pump from the stack 2 Remove the top covers and foam see Removing the Top Cover and Foam on page 131 3 Disconnect all connectors from the main board Connector Description Connector Description 1 Configuration switch J1 GPIB P11 AIV A J2 CAN connector P15 Leak sensor J3 CAN connector P16 Damper J4 RS232 P17 Fan J5 Remote P18 Interface board J6 Analog output P21 Encoder B P2 Power supply P20 AIV B P8 M otor B P22 Solvent Selection Valve P12 M otor A P9 EMPV P14 Encoder A P19 Flow sensor When removing connectors counterhold on connector 3 with one hand 1100 Series Nano Pump Service Manual Repairing the Pump 3
60. 321 FS to inj valve cap 350 mm 25 um for nanoflow sensor G1375 87322 FS to inj valve cap 550 mm 25 um for nanoflow sensor 61375 87323 Figure 30 Flow Sensor Assembly 166 1100 Series Nano Pump Service M anual Parts and Materials 4 Power and Status Light Pipes Table 21 Power and Status Light Pipes Item Description Part Number 1 Light pipe power switch 5041 8382 2 Power switch coupler 5041 8383 3 Light pipe status lamp 5041 8384 4 Power switch button 5041 8381 Figure 31 Powerand Status Light Pipes 1100 Series Nano Pump Service Manual 167 4 Parts and Materials Leak Parts Table 22 Leak Parts Item Description Part Number 1 Holder leak funnel 5041 8389 2 Leak funnel 5041 8388 3 Tube clip 5041 8387 4 Leak plane pump 5041 8390 5 Leak sensor 5061 3356 6 Corrugated waste tube reorder pack 5m 5062 2463 Figure 32 Leak Parts 168 1100 Series Nano Pump Service M anual Parts and Materials 4 Cover Parts Table 23 Cover Parts Item Description Part Number 1 Plastic cover kit includes top base and both sides G1312 68703 2 Front plate 61376 60011 3 Logo plate Agilent 1100 5042 1381 Figure 33 Cover Parts 1100 Series Nano Pump Service Manual 169 4 Parts and Materials Sheet M etal Kit Table 24 Sheet Metal Kit Item Description Part Number 1 Sheet metal kit includes top base and Z panel G1376 68701 2 Screw for cover and Z Panel 5022 2112
61. 4 Typically 1 ml per channel One chamber per channel with optimized flow path for fastest solvent change over PTFE FEP PEEK 1100 Series Nano Pump Service Manual 259 7 Specifications 260 1100 Series Nano Pump Service Manual Agilent 1100 Series Nano Pump Service Manual o e A 009 Safety Information o e General 262 e Safety Symbols 264 Lithium Batteries Information 265 Danish Information 265 Radio Interference 266 Sound Emission 267 Solvent Information 268 Agilent Technologies on Internet 269 The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements a Agilent Technologies 261 A Safety Information General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards This instrument is designed as a laboratory equipment Use it in an analytical environment only Use this instrument in a manner described in this manual Operation Before applying power comply with the installation section Additionally the following must be o
62. 5 Not used 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 Table 52 Remote Signal Distribution continued Pin Signal Description 6 POWER ON H All modules connected to system are switched on Receiver is any module relying on operation of others 7 READY H System is ready for next analysis Receiver is any sequence controller 8 STOP L Request to reach system ready state as soon as possible for example stop run abort or finish and stop injection Receiver is any module performing run time controlled activities 9 START REQUEST L Request to start injection cycle for example by start key on any module Receiver is the autosampler RS 232C The RS 232C connector is used to control the instrument from a computer through RS 232C connection using the appropriate software This connector can be activated by the configuration switch module next to the GPIB connector The RS 232C is designed as DCE Data Communication Equipment with a 9 pin male SUB D type connector The pins are defined as follows Table 53 RS 232C Connection Table Pin Direction Function 1 In DCD 2 In RxD 3 Out TxD 4 Out DTR 5 Ground 6 In DSR 7 Out RTS 8 In CTS 1100 Series Nano Pump Service Manual 221 5 Introduction to the Nano Pump Table 53 RS 232C Connection Table continued Pin Direction Function 9 In RI Instrument PC RX 2 2 RX TX 3 X 3 TX DTR 4 4 DTR enD 5
63. Connect the capillary end into the purge valve holder Adapt the purge valve holder on the pump head of channel A Use the screw and the washer to fix the purge valve holder Insert the purge valve assembly into the purge valve holder and locate the outlet and waste outlet as shown below Use the 14 mm wrench to tighten the purge valve assembly Connect the waste tube from the EMPV assembly to the waste outlet of the purge valve Connect the pump outlet capillary into the outlet of the purge valve 1100 Series Nano Pump Service M anual Repairing the Pump 3 Exchanging the Purge Valve Frit or the Complete M anual Purge Valve Frequency Frit when plunger seals are exchanged or when contaminated or blocked pressure drop of gt 10 bar across the frit at a flow rate of 2500 ul min of H20 with purge valve opened Purge valve if internally leaking Tools required Wrench 1 4 inch Wrench 4 mm depending on pump outlet capillary Wrench 14mm Pair of tweezers or toothpick Parts required PTFE Frit pack of 5 01018 22707 Purge valve G1311 60009 Purge valve holder Pump outlet capillary Y Figure 18 Exchanging the Purge Valve Frit Exchanging the purge valve frit or the complete purge valve 1 Using a 1 4 inch or 4 mm wrench disconnect the pump outlet capillary at the purge valve 1100 Series Nano Pump Service Manual 115 3 116 Repairing the Pump 2 Disconnect the waste tube Beware of leaking solvents due t
64. Failure to produce peaks or abnormally small peaks after injection Possible Causes and Suggested Actions Possible Causes Suggested Actions Notes A leak in a sample carrying area of the system The 40 pl chamber of the micro sampler metering head has developed bubbles Using a flashlight and absorbent tissue carefully check for leaks in the following areas a All micro sampler valve ports b The junction of the needle and sample loop capillary c The needle seat interface d Column inlet and outlet At very low flow rates a leak may never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see e Flow cell inlet capillary at the capillary cell body junction In the user interface diagnostics access the Change Piston function of the micro sampler maintenance positions This function draws the head volume is used At very low metering piston fully inward clearing the chamber e Under flow conditions activate this function for at least 5 minutes The sampler valve must be in flows bubbles may form in the unused space between the metering piston and the chamber wall The bubbles act to defeat the draw of the mainpass position at this time Any bubbles sample into the needle which have formed in the chamber will now be cleared by the flow the mobile phase being pumped should not contain water In most applicati
65. Feedback EM F Maintenance requires the exchange of components in the flow path which are subject to mechanical wear or stress Ideally the frequency at which components are exchanged should be based on the intensity of usage of the instrument and the analytical conditions and not on a predefined time interval The early maintenance feedback EMF feature monitors the usage of specific components in the instrument and provides feedback when the user settable limits have been exceeded The visual feedback in the user interface provides an indication that maintenance procedures should be scheduled EMF Counters The nano pump provides a series of EMF counters for the left and right pump heads Each counter increments with pump use and can be assigned a maximum limit which provides visual feedback in the user interface when the limit is exceeded Each counter can be reset to zero after maintenance has been done The nano pump provides the following EMF counters e liquimeter pump A e seal wear pump A e liquimeter pump B and e seal wear pump B Liquimeters The liquimeters display the total volume of solvent pumped by the left and right pump heads since the last reset of the counters Both liquimeters can be assigned an EMF maximum limit When the limit is exceeded the EMF flag in the user interface is displayed 1100 Series Nano Pump Service Manual 205 5 206 Introduction to the Nano Pump Seal Wear Counters The seal
66. M PV 165 Flow Sensor Assembly 166 Power and Status Light Pipes 167 Leak Parts 168 Cover Parts 169 Sheet Metal Kit 170 Foam Parts 171 Nano Pump Accessory Kit G2226 68705 172 Control M odule G1323B 173 Cable Overview 174 Analog Cables 176 Remote Cables 179 BCD Cables 184 Auxiliary Cable 187 CAN Cable 188 External Contact Cable 189 1100 Series Nano Pump Service M anual ix RS 232 Cable Kit 190 LAN Cables 191 5 Introduction to the Nano Pump Introduction to the Nano Pump 194 Hydraulic Path Overview 195 How Does the Pumping unit Work 197 Electrical Connections 202 Instrument Layout 204 Early Maintenance Feedback EMF 205 EMF Counters 205 Using the EMF Counters 206 The Electronics 207 Nano pump main board NPM 207 Firmware Description 213 Resident System 213 Main System 213 Firmware Updates 214 Optional Interface Boards 215 BCD Board 215 LAN Board 217 Interfaces 218 Analog Signal Output 219 GPIB Interface 219 CAN Interface 219 Remote Interface 220 RS 232C 221 Setting the 8 bit Configuration Switch 223 GPIB Default Addresses 224 1100 Series Nano Pump Service M anual Communication Settings for RS 232C Communication 224 Forced Cold Start Settings 226 Stay Resident Settings 226 The Main Power Supply Assembly 228 6 Control Module Screens for the Nano Pump Major keys on the Agilent 1100 Control Module 232 Screens available from the Analysis view 233 Settings screens 236 Methodscreens
67. M board Suggested Actions V Ensure the damper connector is clean and seated correctly V Exchange the damper V Exchange the CSM board 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Pump Configuration At switch on the pump has recognized a new pump configuration The nano pump is assigned its configuration at the factory If the active inlet valve and pump encoder of channel B are disconnected and the nano pump is rebooted the error message is generated However the nano pump will function as an isocratic pump in this configuration The error message reappears after each switch on Probable Causes e Active inlet valve and pump encoder of channel B disconnected Suggested Actions Y Reconnect the active inlet valve and pump encoder of channel B 1100 Series Nano Pump Service Manual 45 2 46 Troubleshooting and Test Functions Valve Fuse Valve Fuse 0 Channels Al and A2 Valve Fuse 1 Channels B1 and B2 One of the solvent selection valves in the nano pump has drawn excessive current causing the selection valve electronic fuse to open Probable Causes e Defective solvent selection valve e Defective connection cable front panel to CSM board e Defective CSM board Suggested Actions V Restart the nano pump If the error message appears again exchange the solvent selection valve V Exchange the connection cable V Exchange the CSM board 1100 Series Nano Pump Service
68. NC 9 Black Start request Low 1100 Series Nano Pump Service Manual 179 4 180 Parts and Materials Table 34 Agilent 1100 to 3392 3 Integrators Connector Pin Pin SignalName Active 01046 60206 3392 3 Agilent 1100 TTL 3 1 White Digital ground NC 2 Brown Prepare run Low fo 11 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected Y NC 6 Yellow Power on High 9 7 Red Ready High 4 Key 1 8 Green Stop Low NC 9 Black Start request Low Table 35 Agilent 1100 to 3394 Integrators Connector Pin Pin Signal Name Active 01046 60210 3394 Agilent 1100 TTL 9 1 White Digital ground won NC 2 Brown Prepare run Low so D 3 3 Gray Start Low Y NC 4 Blue Shut down Low 2o NC 5 Pink Not connected ie E NC 6 Yellow Power on High O 5 14 7 Red Ready High 6 8 Green Stop Low 1 9 Black Start request Low 13 15 Not connected 1100 Series Nano Pump Service Manual Parts and Materials 4 START and STOP are connected via diodes to pin 3 of the 3394 connector Table 36 Agilent 1100 to 3396A Integrators Connector Pin Pin SignalName Active 03394 60600 3394 Agilent 1100 TTL 9 1 White Digital ground O NC 2 Brown Prepare run Low o 15 3 3 Gray Start Low NC 4 Blue Shut down Low rs NC 5 Pink Not connected i NC 6 Yellow Power on High O0 5 14 7 Red Ready High 1 8 Green Stop
69. Nano Pump Service M anual Repairing the Pump 3 Assembling the Main Cover Frequency If cover is broken Tools required None Parts required Cover kit G1312 68703 includes base top left and right The cover kit contains all parts but it is not assembled WARNING In case you insert the left or right side in the opposite position you may not be able to remove the side from the top part 1 Place the top part on the bench and insert the left 2 Replace the cover and right side into the top part 3 Replace the nano pump into the stack and reconnect the cables and capillaries 4Turn on the nano pump 1100 Series Nano Pump Service Manual 153 3 Repairing the Pump Replacing the Top Cover and Foam Tools required Screwdriver Pozidriv 1 Preparations for this M ake sure that after your repair all assemblies cables capillaries and procedure connectors are located in its correct place 1 Place the damper cable in a curve to the right side towards the active inlet valve cable of pump B Replace the top foam section 2 Make sure that the foam is installed correctly and is located in the safety light switch SS A Light switch 4 Replace the optional interface board or the board cover plate 154 1100 Series Nano Pump Service M anual Repairing the Pump 3 5 Replace the metal cover slide the metal tabs into place 1 un
70. On Time EMF Controller G1323B DE50174451 B 02 01 Od 00 00h ap Pump G1376A DE01700107 A 04 01 Od 01 13h Autosampler G1313A DE91610583 A 04 01 Od 02 10h Col Comp G1316A DE91613536 A 04 01 Od 02 10h EMF Use the F1 key EMF to set EMF Limits Choose menu item Setup Limits to Early Mainte set the amount of pumped solvent or seal wear when you want to receive a nance Feedback warning for exceeded limits Pump O Temp O uauc 0 OOBGIE Module Product Serial Version On Time EMF Controller G1323B DE01704451 B 02 01 Od 00 00h Cap Pump GT376A BE01700107 A 04 01 Od 01 13h Autosampler G1313A DE91610583 A 04 01 Od 02 10h Col Comp G1316A DE91613536 A 04 01 Od 02 10h 4 Setup Limits i 2 Show Events Print Danes y ee LRecords Press F7 Reset to reset the counters after exchanging parts that are subject to wear Changes must be activated with F6 Done 248 1100 Series Nano Pump Service Manual Control Module Screens forthe Nano Pump 6 Pump L Temp L RJ G1376A Capillary Pump Serial DE01700107 Pump A Pump B Solvent pumped 0 84 0 84 Limit OFFI Seal Wear 255 149 Limit If a set limit has been exceeded a message box will pop up An exceeded limit will not stop a sequence or run information only to plan maintenance activities If you press Reset the limits will be removed Ignore will continue to keep the EMF flag set Records Fiscal Pumped solvent limit exceede
71. Pump 106 Using the ESD Strap 106 Overview aboutthe Nano Pump 107 Simple Repair Procedures 108 Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve 109 Exchanging the Outlet Ball Valve Sieve or the Complete Valve 112 Installing the Manual Purge Valve 114 Exchanging the Purge Valve Frit or the Complete Manual Purge Valve 115 Exchanging the EMPV Assembly 117 Exchanging the Solvent Selection Valve 118 Removing and Disassembling the Pump Head Assembly 120 Exchanging the Pump Seals and Seal Wear in Procedure 122 Exchanging the Plungers 125 Exchanging the Flow Sensor 126 Reassembling the Pump Head Assembly 127 Exchanging the Optional Interface Board 129 Exchanging Internal Parts 130 Removing the Top Coverand Foam 131 Exchanging the Nano Pump Main Board NPM Board 134 Exchanging the Damper 141 Exchanging the Fan 143 Exchanging a Pump Drive 145 Exchanging the Power Supply 147 Exchanging the Leak Sensor 149 og Agilent Technologies ave 3 Repairing the Pump Exchanging Status Light Pipe 152 Assembling the Main Cover 153 Replacing the Top Cover and Foam 154 This chapter includes Instruction on how to repair the pump 104 1100 Series Nano Pump Service Manual Introduction Repairing the Pump 3 Simple Replacements The nano pump is designed for easy repair The most frequent repairs such as plunger seal change and filter frit change can be done with the nano pump in place in the system stack These repairs
72. Service M anual Repairing the Pump 3 Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve Frequency If internally leaking backflow Tools required Wrench 14 mm Parts required Active inlet valve G1312 60010 Valve Cartridge 5062 8562 Removing the Active Inlet Valve 1 Unplug the active inlet valve cable from the connector 2 Disconnect the solvent inlet tube at the inlet valve Beware of leaking solvents 3 Using a 14 mm wrench loosen the active inlet valve and remove the valve from pump head Valve body ep O O Valve cartridge Figure 14 Active Inlet Valve Parts Exchanging the Valve Cartridge 1 Using a pair of tweezers remove the valve cartridge from the actuator assembly 1100 Series Nano Pump Service M anual 109 3 Repairing the Pump 2 3 Before inserting the new valve cartridge clean the area in the actuator assembly Fill a syringe with alcohol and flush the cartridge area thoroughly Insert a new valve cartridge into the actuator assembly Make sure the valve cartridge is fully inserted into the actuator assembly Replacing the Active Inlet Valve 1 Insert the new valve into the pump head Using the 14 mm wrench turn the nut until hand tight Position the valve that the solvent inlet tube connection points towards the front Using the 14 mm wrench tighten the nut by turning the valve in its final position not more than a quarter turn Do not overtighten the valve The solvent in
73. a line voltage of 100 240 volts AC 10 with a line frequency of 50 or 60 Hz Maximum power consumption is 220 VA Volt Amps There is no voltage selector on your nano pump because the power supply has wide ranging capability There are no externally accessible fuses because automatic electronic fuses are implemented in the power supply The security lever at the power input socket prevents that the nano pump cover is taken off when line power is still connected e The interface board slot is used for external contacts BCD output LAN and for future use WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations 202 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 Configuration switch Slot for interface board j l RS232 Sy SS Remote m Analog output o CAN Bus o K at pi ak a a D P A ane Hz 60 60 gt C gt C gt gt lt gt a 2 2 TARE x A a C 3 c St gt F 5 saae E a A lV ni erite aC Er e gt C pia 5 dun LLL pohly n a a AUNT NTA i WEY TOA GL x 2q pRa 3x J m Y Power Plug Security Lever
74. aintenance of flow path components e g pump seals injection seal to prove pressure tightness up to 400 bar The test involves monitoring the pressure profile as the nano pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness of the system Normal Mode Pressure Test on page 68 Leak Test The leak test is a diagnostic test designed to determine the pressure tightness of the nano pump Especially when a problem with the pistons the AIV or the OBV is suspected use this test to help troubleshoot the nano pump and its pumping performance Leak Test on page 73 EM PV Test The test is designed to verify the performance of the EMPV The test must always be done when the EMPV valve is exchanged The test should also be done if column flow stability problems occurs in micro mode see The EMPYV test is not a substitute for the leak test or pressure test The leak and pressure tests should also be done when leaks within the pump heads might be the problem EMPV Test on page 84 Flow Sensor Accuracy Calibration The flow sensor accuracy calibration test is designed to calibrate the flow accuracy and to compensate the electronic offset of the nano flow sensor This test should be done if high flow accuracy is needed at flow rates lower than 500 nl min The calibration of the flow sensor is based in the linear relationship between the flow rate and pressure drop
75. als injection seal to prove tightness up to 400 bar The test involves monitoring the pressure profile as the nano pump runs through a predefined pumping sequence The resulting pressure profile provides information about the pressure tightness of the system The injection device outlet port 6 is blocked with a blank nut and then the test is run using isopropyl alcohol IPA while monitoring the pressure profile using an integrator on the analog output or in the plot screen in the ChemStation The pressure profile is shown in Figure 8 Step 2 T T T T T T T 0 0 5 1 15 2 2 5 3 a35 Time minutes Figure8 Typical Pressure Test Pressure Profile with IPA The test begins with the initialization of both pumpheads After initialization plungers Al and B1 are both at the top of their stroke Next pump A begins pumping solvent with a flow rate of 510 ul min and stroke of 100 ul The nano pump continues to pump until a system pressure of 390 bar is reached When the system pressure reaches 390 bar the nano pump switches off The pressure drop from this point onwards should be no more than 2 bar minute 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 For this test only channel A2 is active To test the pressure tightness of the pump component use the leak test see Leak Test on page 73 Positioning the blank nut If a specific component is suspected of causing a system leak place the blank
76. als from the two temperature sensors leak sensor and board mounted temperature compensation sensor are used by the leak algorithm to determine whether a leak is present When a leak occurs the leak sensor is cooled by the solvent This changes the resistance of the leak sensor which is sensed by the leak sensor circuit on the CSM board Probable Causes e Loose fittings e Broken capillary e Loose or leaking active inlet valve outlet ball valve or EMPV e Defective pump seals Suggested Actions Y Ensure all fittings are tight V Exchange defective capillaries V Ensure pump components are seated correctly If there are still signs of a leak exchange the appropriate seal active inlet valve outlet ball valve V Exchange the pump seals 1100 Series Nano Pump Service Manual 31 2 32 Troubleshooting and Test Functions Leak Sensor Open The leak sensor in the nano pump has failed open circuit The current through the leak sensor is dependent on temperature A leak is detected when solvent cools the leak sensor causing the leak sensor current to change within defined limits If the current falls outside the lower limit the error message is generated Probable Causes e Leak sensor not connected to the CSM board e Defective leak sensor e Leak sensor incorrectly routed being pinched by a metal component Suggested Actions V Ensure the leak sensor is connected correctly V Exchange the leak sensor 1100
77. ameters using the same 8 bit configuration switch twice for example for both GPIB and RS 232C GPIB Default Addresses If you just want to change the GPIB address and need a detailed procedure refer to the Installing Your Agilent ChemStation System handbook Default GPIB address is set to the following addresses Table 55 Default Addresses for Agilent Series 1100 M odules Module Address Binary Address Pump 22 00010110 FLD 23 00010111 VWD 24 00011000 Agilent 8453A 25 00011101 DAD MWD 26 00011010 Column compartment 27 00011011 Autosampler 28 00011100 Well plate sampler no address 000000000000 RID 29 00011101 where 0 means that the switch is down and 1 means that the switch is up Communication Settings for RS 232C Communication The communication protocol used in this instrument supports only hardware handshake CTS RTS 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 Switches 1 in down and 2 in up position define that the RS 232C parameters will be changed Once the change has been completed the instrument must be powered up again in order to store the values in the non volatile memory Table 56 Communication Settings for RS 232C Communication Mode Select 1 2 3 4 5 6 7 8 RS 232C 0 1 Baud rate Data Parity Bits Use the following tables for selecting the setting which you want to use for RS 232C communication The number 0 means that the switch is down
78. an the outlet valve in channel A Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Ensure the pump head screws in channel A are tight Exchange the pump seals in channel A Check the plunger for scratches Exchange if scratched 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Second plateau negative or unstable and at least one other plateau positive 3004 ooo ee 200 J n Potential Cause Corrective Action Leaking outlet valve in channel B Clean the outlet valve in channel B Ensure the sieve in the outlet valves are installed correctly Tighten the outlet valve Loose pump head screws in channel B Ensure the pump head screws in channel B are tight Leaking seal or scratched plunger in channel B2 Exchange the pump seals in channel B Check the plunger for scratches Exchange if scratched 81 1100 Series Nano Pump Service M anual 2 82 Troubleshooting and Test Functions Third plateau negative or unstable and at least one other plateau positive 300 4 260 4 1504 104 Potential Cause Corrective Action Air in channel A or new seals not yet seated Loose active inlet valve in channel A Loose pump head screws in channel A Loose outlet valve in channel A Leaking seal or scratched plunger in channel A1 Defective active inlet valve in channel A Flush channel A thoroughly with isopropanol unde
79. and 1 means that the switch is up Table 57 Baud Rate Settings Switches Baud Rate Switches Baud Rate 3 4 5 3 4 5 0 0 0 9600 1 0 0 9600 0 0 1 1200 1 0 1 14400 0 1 0 2400 1 1 0 19200 0 1 1 4800 1 1 1 38400 Table 58 Data Bit Settings Switch6 Data Word Size 0 7 Bit Communication iL 8 Bit Communication Table 59 Parity Settings Switches Parity 7 8 0 0 No Parity 1100 Series Nano Pump Service M anual 225 5 Introduction to the Nano Pump Table 59 Parity Settings continued 1 0 Odd Parity L 1 Even Parity One start bit and one stop bit are always used not selectable Per default the module will turn into 19200 baud 8 data bit with no parity Forced Cold Start Settings Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation Forced cold start erases all methods and data stored in the non volatile memory Exceptions are diagnose and repair log books which will not be erased If you use the following switch settings and power the instrument up again a forced cold start has been completed Table 60 Forced Cold Start Settings Mode Select 1 2 3 4 b 6 7 8 TEST BOOT l 1 0 0 0 0 0 1 To return to normal operation set switche
80. ange 258 remote connector 202 removing the foam 131 removing the pump head 120 removing the top cover 131 repair procedures 108 replacing the foam 154 replacing the top cover 154 restart without cover 38 results pressure test 71 RS 232 cable kitto PC 190 RS 232C communication settings 224 interface 221 Settings 225 RS 232C connector 202 run mode 24 running the leak test 74 running the pressure test 69 S safety standards 4 safety information 261 onlithium batteries 265 safety light switch 154 screw driver pozidriv 1 118 126 141 143 145 147 149 152 154 seal wear counter 123 206 seals 108 security lever 105 selection valve failed 42 selection valve fuse 46 serial number entered on control module 252 serial number change with control module 252 servo restart failed 53 setable flow range 258 sheet metal kit 170 shipping container 5 shut down 28 sieve 108 112 1100 Series Nano Pump Service Manual Index simple repair procedures 108 site requirements 2 solvent channels 17 solvent information 268 solvent selection valve 118 solvent selection valve drive 207 sonic bath 112 sound emission 267 specification performance 258 physical 3 spectrum range 241 stack configuration 7 stack configuration front view 8 stack configuration rear view 9 Status light pipe 152 167 stroke length 62 stroke volume 200 synchronization lost 29 T temperature
81. ano Pump 65 Basic System Troubleshooting 92 This chapter describes the pump s built in troubleshooting and test functions Status Indicators The nano pump is provided with two status indicators which indicate the operational state prerun run and error states of the nano pump The status indicators provide a quick visual check of the operation of the nano pump Status Indicators on page 23 Error Messages In the event of an electronic mechanical or hydraulic failure the nano pump generates an error message in the user interface The following pages describe the meaning of the error messages For each message a short description of the failure a list of probable causes of the problem and a list of suggested actions to fix the problem are provided Error Messages on page 25 Micro Pressure Test This is a fast test to verify the tightness of a micro system where the pump is operating in the micro mode and no manual purge valve is installed The pump is operating in the pressure control mode at 380 bar for several minutes The remaining flow in the column flow path between the EMPV and the blank nut is measured Testing your Nano Pump on page 65 5 a Agilent Technologies 21 2 22 Troubleshooting and Test Functions Pressure Test The pressure test is a quick built in test designed to demonstrate the pressure tightness of the system The test should be used when problems with leaks are suspected or after m
82. ano pump Do not let liquid drip into the nano pump It could cause shock hazard and it could damage the nano pump Using the ESD Strap 106 Electronic boards are sensitive to electrostatic discharge ESD In order to prevent damage always use an ESD strap supplied in the standard accessory kit see Nano Pump Accessory Kit G2226 68705 on page 172 when handling electronic boards and components Using the ESD Strap 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 12 Using the ESD Strap 1100 Series Nano Pump Service M anual Repairing the Pump 3 Overview about the Nano Pump Figure 13 shows the main assemblies of the nano pump The pump heads and its parts do require normal maintenance for example seal exchange and can be accessed from the front simple repairs Replacing internal parts will require removing the module from its stack and to open the top cover Vv ey px wy hy Figure 13 Overview of Repair Procedures 1 Pumpdrive A 8 Main Board 2 Damper 9 Power Supply 3 EM PV 10 Fan 4 Outlet Ball Valve A 11 Flow Sensor 5 Pumphead A 12 Pumphead B 6 Solvent Selection Valve 13 Active Inlet Valve B 7 Leak Sensor 1100 Series Nano Pump Service M a
83. are described in Simple Repair Procedures on page 108 When opening capillary or tube fittings solvents may leak out Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used Exchanging Internal Parts Some repairs may require exchange of defective internal parts Exchange of these parts requires removing the nano pump from the stack removing the covers and disassembling the nano pump The security lever at the power input socket prevents that the pump cover is taken off when line power is still connected To prevent personal injury the power cable must be removed from the nano pump before opening the cover Do not connect the power cable to the nano pump while the covers are removed Electronic boards and components are sensitive to electrostatic discharge ESD In order to prevent damage always use an ESD protection for example the ESD wrist strap from the accessory kit when handling electronic boards and components 1100 Series Nano Pump Service Manual 105 3 Repairing the Pump Cleaning the Nano Pump The nano pump case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth as liquid can drop into the n
84. are printed You have now the possibility to accept and store all the correction factors and the measured offset accept only the measured offset reset no correction factor or offset are applied 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions Test evaluation __Acceptall Accept Offset Only _Reset__ __ Close Date 6 25 02 Time 4 34 35PM PP Pressure drop overfiter Taf E Offset flow pressure sensor 026 ymin ba oo Calibration step 1 T Calibration step 200 88 1 Calibration step 3 88 0 Calibration step 0 888 Calibration step 5 P88 2 Figure 11 Flow sensor accuracy calibration test evaluation Table5 Flow sensor accuracy calibration test evaluation 2 Pressure drop over filter The measured value should not be higher If higher we recommend to than 10 Bar change the filter in front of the EM PV and restart the test again Offset flow This value can be positive or negative If higher or lower replace the and should be between 500 nL min and nano flow sensor 90 nL min Offset pressure sensor This value has not to be considered in the evaluation 1100 Series Nano Pump Service Manual 89 2 Troubleshooting and Test Functions Table5 Flow sensor accuracy calibration test evaluation continued Correction factor e Step 1 Between 95 and 105 e If value out of range check Step 5 Between 80 and 500 the restriction capillary for Step 2 4 Continu
85. bserved Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth via a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuseholders must be avoided Any adjustment maintenance and repair of the opened instrument under voltage is forbidden Disconnect the instrument from the line and unplug the power cord before maintenance 262 Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard 1100 Series Nano Pump Service Manual Safety Information A Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though the instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious pe
86. capillary on the injection device port 1 the connector 4 Unclip the mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 135 6 Remove both pump assemblies For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the connector P8 P21 and P12 P14 at the main board 1100 Series Nano Pump Service Manual 149 3 150 Repairing the Pump 7 Disconnect the fan cable P17 at the main board 8 Remove the solvent selection valve See exchanging the solvent selection valve in this chapter 9 Remove the CSPM board Disconnect the remaining connectors see exchanging the high pressure pump main board in this chapter 10 Push the leak sensor cable through the recess of the solvent selection valve and lift out the bottom foam 11 Unclip the leak pan and place it in front of the nano pump 12 Pull the leak sensor out of the leak pan and lift the cable out of the metal plate 13 Place the new leak sensor into the leak pan see Figure 24 and replace the pan Make sure that the cable is located in the recess of the bottom metal plate 14 Reinstall the bottom foam Make sure that the leak senor cable is guided through the foam to th
87. capillary on the injection device port 1 the connector 4 Unclip the mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 135 6 Remove pump assembly B For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the connector P8 P21 at the main board Unclip the mixing chamber from its holder 7 Disconnect the fan cable P17 at the main board and slide the fan towards the front and move it out of the unit 1100 Series Nano Pump Service Manual 143 3 144 Repairing the Pump 8 Place the new fan into the recess Make sure that the air flow is directed as indicated arrow on the fan points in the same direction as on the foam Connect the cable to the main board P17 9 Re install pump assembly B Reconnect all capillaries and connectors P8 P21 10 Re install the Z panel and the damper Reconnect the capillaries and connectors P16 P11 P20 11 Re install the mixing chamber to its holder 12 Re install the flow sensor Reconnect the capillaries and the cable 13 Re install the EMPV Reconnect the capillaries the waste tube and the cable 14 Replace the top foam section optional interface board front cover and top cover
88. chromatographically b significant area of the system c Capillaries which are internally broken especially those capillaries located in a chromatographically significant area carefully check for leaks throughout the system especially in the following areas All micro sampler valve ports Column inlet and outlet Flow cell inlet capillary at the capillary cell body junction Refer to chapter 3 for information on connecting capillaries M ake sure the capillary connections are correctly made throughout the system especially in the following areas All micro sampler valve ports Column inlet and outlet Flow cell inlet capillary at the capillary cell body junction Refer to chapter 3 for advice on diagnosing an internally broken capillary Check capillaries for an internal break particularly the needle seat capillary the sampler valve to column capillary and the flow cell inlet capillary never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see Capillaries which have been crushed by module covers are often broken internally and may show no external evidence of a break 98 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions Failure to produce peaks or abnormally small peaks after injection 2 There are no peaks or the peak size is significantly below the typical peak size for this method with this column Table 11
89. cm long x 1 8710 2411 Hex key 4 0 mm 15 cm long T handle x 1 8710 2392 Torque adapter G1315 45003 CAN cable 1 m long 5181 1519 Purge valve assembly G1311 60009 Purge valve holder 61312 23200 Screw for the purge valve holder 0515 0175 FS to Inj valve cap 350 mm 25 um G1375 87322 FS to Inj valve cap 550 mm 25 um G1375 87323 Flow sensor accuracy calibration capillary 8000 mm 25 um G2226 67300 ESD wrist strap 9300 1408 6 1100 Series Nano Pump Service Manual Installing the Pump 1 Optimizing the Stack Configuration If your nano pump is part of a complete 1100 series system you can ensure optimum performance by limiting the configuration of the system stack to the following configuration This configuration optimizes the system flow path ensuring minimum delay volume If a single stack configuration becomes too high e g if an additional module like a G1330B ALS Thermostat is added or if your bench is too high a two stack configuration may be a better setup Separate the stack between pump and sampler and place the stack containing the pump on the right side of the stack containing the sampler 1100 Series Nano Pump Service Manual 7 1 Installing the Pump
90. cted to pin 6 hea 5 Shield Analog i BRN 6 Connected to pin 4 7 Key 8 Not connected 176 1100 Series Nano Pump Service Manual Parts and Materials 4 Table 30 Agilent 1100 to 3394 6 Integrators Connector Pin Pin Signal Name 35900 60750 3394 6 Agilent 1100 1 Not connected 2 Shield Analog 3 Center Analog ta Table 31 Agilent 1100 to BNC Connector Connector Pin Pin Signal Name 8120 1840 BNC Agilent 1100 Shield Shield Analog Center Center Analog 1100 Series Nano Pump Service Manual 177 4 Parts and Materials Table 32 Agilent 1100 to General Purpose Connector Pin Pin Signal Name 01046 60105 3394 6 Agilent 1100 1 Not connected 2 Black Analog Pas 3 Red Analog 178 1100 Series Nano Pump Service Manual Parts and Materials 4 Remote Cables 00000 0 0000 One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1100 Series modules The other end depends on the instrument to be connected to Table 33 Agilent 1100 to 3390 Integrators Connector Pin Pin Signal Name Active 01046 60203 3390 Agilent 1100 TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low D NC 4 Blue Shut down Low NC 5 Pink Not connected Q NC 6 Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low
91. d The amount of solvent delivered by the pump channel A for binary pump has exceeded its defined limit J Reset System Error Use the F2 key System Log or F3 key Error Log to look for errors on the Log selected highlighted module 1100 Series Nano Pump Service Manual 249 6 Control Module Screens for the Nano Pump Date Time 05 03 00 16 08 39 05 03 00 16 08 22 05 03 00 16 08 18 05 03 00 16 01 56 Maintenance Log Use the F4 key Maint Log to review and enter maintenance activities in the maintenance logbooOk Records Miser Message AlV B replaced 02 03 00 03 45 33 AIV A replaced 02 03 00 03 45 18 Pump A seals replaced 02 03 00 11 43 14 and of logbook Use the F7 key Add to add new maintenance activities You can add a comment to the activity into the line Add using the control modules alphanumeric keys Changes must be activated with F6 Done Records Ee Lamp Wee 0 00 iE Ready AIV j Pump A pistons replaced Pump B seals replaced 250 1100 Series Nano Pump Service M anual Control Module Screens forthe Nano Pump 6 Firmware Update Use the Esc key to receive Views on the F5 key Choose System from the pull down menu Use the F3 key Records to select the pump Use the F5 key FW Update to enter the Update section If you want to update the resident firmware together with specific main firmware revisions select the file from the
92. degasser is an exception A vacuum degasser can be connected via the APG remote connector to the other modules of the stack The AUX output allows the user to monitor the vacuum level in the degasser chamber An Agilent 1100 Series control module can be connected to the CAN bus at any of the modules in the system except for the degasser The Agilent ChemStation can be connected to the system through one GPIB or LAN requires the installation of a LAN board cable at any of the modules except for the degasser preferably at the detector M UST for the DAD For more information about connecting the control module or Agilent ChemStation refer to the respective user manual For connecting the Agilent 1100 Series equipment to non Agilent 1100 Series equipment see Introduction to the Nano Pump on page 193 4 Connect the pre terminated end of the pump to sampler capillary G1375 87322 or G1375 87323 to the flow sensor outlet of the pump Connect the other end of this capillary to port 1 of the sampler injection valve Carefully route all capillaries so that they are not crushed or broken by module front covers Avoid excessive bending 5 Connect the 1 8 inch plastic EMPV waste tube to the barbed waste fitting of the EMPV Route the waste tube to an appropriate waste container The pump was shipped with default configuration settings To change these settings see Setting the 8 bit Configuration Switch on page 223 To disconnect a module fro
93. derneath the Z Panel in the front then lower the back of the metal plate 2 and fix the two holding screws 6 Replace the top cover 8 Replace the leak funnel with the waste tube Locate the lower end of the waste tube in the holder of the leak pan Waste funnel Leak pan Waste tube holder 1100 Series Nano Pump Service Manual 155 3 Repairing the Pump 9 Place the nano pump back to its position in the stack replace solvent cabinet and reconnect all tubes and capillaries figure shows configuration with solvent selection valve 10 Replace the front covers and reconnect the power cable to the nano pump 11 Switch on the nano pump 156 1100 Series Nano Pump Service Manual Agilent 1100 Series Nano Pump Service Manual 4 Parts and Materials Nano Pump Main Assemblies 158 Solvent Cabinet and Bottle Head Assembly 160 Nano Pump Hydraulic Path 161 Pump Head Assembly 163 Electro Magnetic Proportional Valve EMPV 165 Flow SensorAssembly 166 Power and Status Light Pipes 167 Leak Parts 168 Cover Parts 169 Sheet Metal Kit 170 Foam Parts 171 Nano Pump Accessory Kit G2226 68705 172 Control M odule G1323B
94. dicated page For each one possible causes are discussed and actions to solve the problem are suggested Time out 27 Shutdown 28 Remote Time out 29 Synchronization Lost 30 Leak 31 Leak Sensor Open 32 Leak Sensor Short 33 Compensation Sensor Open 34 Compensation Sensor Short 35 Fan Failed 36 Open Cover 37 Restart Without Cover 38 Zero Solvent Counter 39 Pressure Above Upper Limit 40 Pressure Below Lower Limit 41 Pressure Signal Missing 42 Valve Failed 43 Missing Pressure Reading 44 Pump Configuration 45 Valve Fuse 46 Inlet Valve Fuse 47 Temperature Out of Range 48 Temperature Limit Exceeded 49 Motor Drive Power 50 Encoder Missing 51 Inlet Valve Missing 52 Electro M agnetic Proportional Valve EMPV Missing 53 1100 Series Nano Pump Service Manual 25 2 26 Troubleshooting and Test Functions Flow sensor missing 54 Unsupported Flow Sensor 55 Leak Sensor Missing 56 Servo Restart Failed 57 Pump Head Missing 58 Index Limit 59 Index Adjustment 60 Index Missing 61 StrokeLength 62 Initialization Failed 63 Wait Time out 64 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Time out The time out threshold was exceeded Probable Causes e The analysis was completed successfully and the time out function switched off the nano pump as requested e A not ready condition was present during a sequence or multiple injection run for a period longer than the time out threshold Suggest
95. duction to the Nano Pump An introduction to the nano pump instrument overview theory of operation external communication and internal connectors Control M odule Screens for the Nano Pump Introduction to the screens available for operation of the Agilent 1100 Series nano pump with the control module Specifications Performance specifications of the nano pump For any information about running and optimizing your nano pump please refer to the nano pump user manual 1100 Series Nano Pump Service Manual iii 1100 Series Nano Pump Service Manual Contents 1 Installing the Pump Site Requirements 2 Power Consideration 2 Power Cords 2 Bench Space 3 Environment 3 Unpacking the Nano Pump 5 Optimizing the Stack Configuration 7 Installing the Nano Pump 12 Installing the Nano Pump 13 Install the Degasser G1379A 15 Install the Solvent Cabinet 15 Get the System Ready for the First Injection 17 Manually Priming the Solvent Channels 17 Purging the Pump 18 2 Troubleshooting and Test Functions Status Indicators 23 Power Supply Indicator 23 Instrument Status Indicator 24 Error Messages Indication 24 Error Messages 25 Time out 27 Shutdown 28 Remote Time out 29 1100 Series Nano Pump Service M anual vi Synchronization Lost 30 Leak 31 Leak Sensor Open 32 Leak Sensor Short 33 Compensation Sensor Open 34 Compensation Sensor Short 35 Fan Failed 36 Open Cover 37 Restart Without Cover 38 Zero Solvent Counter 39
96. e P N 5183 4649 10 feet long For standard network connections using a hub use category 5 UTP cables P N G1530 61480 8 m long 1100 Series Nano Pump Service Manual 217 5 Introduction to the Nano Pump Interfaces The Agilent 1100 Series modules provide the following interfaces Table 50 Agilent 1100 Series Interfaces Interface Type Pumps Autosampler Well plate DA Detector VW Detector Thermostatted Vacuum Sampler MW RI Detector Column Degasse Detector Compartment r FL Detector CAN Yes Yes Yes Yes Yes Yes No GPIB Yes Yes No Yes Yes Yes No RS 232C Yes Yes Yes Yes Yes Yes No Remote Yes Yes Yes Yes Yes Yes Yes Analog Yes No No 2x 1x No Yest Interface board Yes Yes Yes Yes Yes No No The well plate sampler can be controlled through CAN via a detector that is connected via GPIB to a ChemStation t The vacuum degasser will have a special connector for specific use For details see description of main board e CAN connectors as interface to other Agilent 1100 Series modules e GPIB connector as interface to the Agilent ChemStation e RS 232C as interface to a computer e REMOTE connector as interface to other Agilent products e Analog Output connector s for signal output and e Interface slot for specific interfacing external contacts BCD LAN and so on For identification and location of the connectors Figure 5 on page 13 WARNING Never use cables other than the ones supplied by Agilent Technologie
97. e Manual Parts and Materials 4 Control M odule G1323B Table 27 Control M odule Parts Item Description Part Number Control Module replacement part including cable G1323 67001 Plastic Housing Kit includes front back and a clamp 5062 8583 CAN cable Agilent 1100 module to control module G1323 81600 Figure 36 Control Module 1100 Series Nano Pump Service Manual 173 4 Parts and Materials Cable Overview WARNING Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Table 28 Cables Overview Type Description Part Number Analog 3390 2 3 integrators 01040 60101 cables 3394 6 integrators 35900 60750 Agilent 35900A A D converter 35900 60750 General purpose spade lugs 01046 60105 Remote 3390 integrator 01046 60203 cables 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series integrator 03394 60600 3396 Series Il 3395A integrator see page 181 3396 Series III 3395B integrator 03396 61010 HP 1050 modules HP 1046A FLD 5061 3378 HP 1046A FLD 5061 3378 Agilent 35900A A D converter 5061 3378 HP 1040 diode array detector 01046 60202 HP 1090 liquid chromatographs 01046 60202 Signal distribution module 01046 60202 BCD 3392 3 integrators 18594 60510 cables not possible with the well plate samplers 174 1100 Series Nano Pump Service Manual
98. e is generated Probable Causes e Flow path blockage in front of the damper e Blocked outlet ball valve e High friction partial mechanical blockage in the drive assembly e Defective drive assembly e Defective CSPM board Suggested Actions V Ensure the capillaries and frits between the pump head and damper inlet are free from blockage V Exchange the outlet ball valve V Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or drive assembly V Exchange the drive assembly V Exchange the CSM board 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Encoder Missing Encoder Missing Pump channel A B Encoder Missing Pump channel B The optical encoder on the pump motor in the nano pump is missing or defective The processor checks the presence of the pump encoder connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable Causes e Defective or disconnected pump encoder connector e Defective pump drive assembly Suggested Actions V Ensure the connector is clean and seated correctly V Exchange the pump drive assembly 1100 Series Nano Pump Service Manual 51 2 52 Troubleshooting and Test Functions Inlet Valve Missing Inlet Valve Missing Pump channel A B Inlet Valve Missing Pump channel B The active inlet valve in the nano pump is missing or defective The proces
99. e top side 15 Replace the main board see Exchanging the Nano Pump Main Board NPM Board on page 134 16 Re install the solvent selection valve 17 Re install the two pump head Reconnect the capillaries and the cable 18 Re install the Z panel and the damper Reconnect the capillaries and connectors P16 P11 P20 19 Re install the mixing chamber to its holder 20 Re install the flow sensor Reconnect the capillaries and the cable 21 Re install the EMPV Reconnect the capillaries the waste tube and the cable 22 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 154 1100 Series Nano Pump Service M anual Repairing the Pump 3 To main board J 23 j Leak sensor Figure 24 Exchanging the Leak Sensor 1100 Series Nano Pump Service M anual 151 3 Repairing the Pump Exchanging Status Light Pipe Frequency Tools required Parts required If part is broken Screwdriver Pozidriv 1 Status light pipe 5041 8384 Preparation for this procedure Remove the front cover and top cover see Removing the Top Cover and Foam on page 131 1 The status light pipe is clipped into the top cover 2 Replace the top cover see Replacing the Top Cover and Foam on page 154 Replace the nano pump into the stack and reconnect the cables and capillaries Turn on the nano pump 152 1100 Series
100. eaking from a module Please notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test where not within the specified limits If the pressure test fails e Ensure all fittings between the pump and the blank nut are tight Repeat the pressure test Often it is only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened If the test fails again insert the blank nut at the outlet of the previous module in the stack and repeat the pressure test Exclude each module one by one to determine which module is leaking e Ifthe pump is determined to be the source of the leak run the EMPV cleaning procedure repeat the pressure test and if the test fails again run the leak test 1100 Series Nano Pump Service Manual 71 2 72 Troubleshooting and Test Functions Potential Causes of Pressure Test Failure After isolating and fixing the cause of the leak repeat the pressure test to confirm the system is pressure tight Potential Cause Pump Corrective Action Loose or leaky fitting Tighten the fitting or exchange the capillary Damaged pump seals or plungers Run the leak test
101. ease notice the difference between an error in the test and a failure of the test An error means that during the operation of the test there was an abnormal termination If a test failed this means that the results of the test where not within the specified limits Often itis only a damaged blank nut itself poorly shaped from overtightening that causes a failure of the test Before investigating on any other possible sources of failure make sure that the blank nut you are using is in good condition and properly tightened The pressure plots shown below are examples only The plots may vary depending on the type and degree of leakage 1100 Series Nano Pump Service M anual 77 2 78 Troubleshooting and Test Functions No pressure increase or minimum pressure of plateau 1 not reached Potential Cause Corrective Action Pump not running Wrong solvent line connections to solvent selection valve Loose or leaky fittings Large leaks visible at the pump seals Large leaks visible at active inlet valve outlet valve Check the logbook for error messages Ensure the solvent lines from the degasser to the solvent selection valve are connected correctly Ensure all fittings are tight or exchange capillary Exchange the pump seals Ensure the leaky components are installed tightly Exchange the component if required Pressure limit not reached but plateaus horizontal or positive Potential Cause
102. ection for the analog pressure output signal The output voltage is 2 mV bar Leak Converter This block comprises a PTC for the leak identification and a NTC for the ambient temperature measurement This assures that temperature changes are not identified as leak A leak would cool down the PTC and its change in resistance results in a leak signal Fan Drive The revolution of the fan is controlled by the main processor depending on the internal heat distribution in the nano pump The fan provides a PWM signal which is proportional to the revolution This fan status signal is used for diagnostics Electronic Fuses The valve circuits are electronically fused on the board Any error on the board or shortages of the valves will activate the electronic fuses that will switch off the supply voltage This prevents the damage of components Onboard Battery An onboard lithium battery buffers the electronic memories when the nano pump is turned off For safety information on lithium batteries see Lithium Batteries Information on page 265 Interfaces The nano pump provides the following interfaces e two CAN connectors as interface to other Agilent 1100 Series modules 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 e one GPIB connector as interface to the Agilent ChemStation e one RS 232C as interface to a computer e one REMOTE connector as interface to other Agilent products e one analog output
103. ed Actions V Check the logbook for the occurrence and source of a not ready condition Restart the analysis where required 1100 Series Nano Pump Service Manual 27 2 28 Troubleshooting and Test Functions Shutdown An external instrument has generated a shut down signal on the remote line The nano pump continually monitors the remote input connectors for status signals A LOW signal input on pin 4 of the remote connector generates the error message Probable Causes e Leak detected in another module with a CAN connection to the system e Leak detected in an external instrument with a remote connection to the system e Shut down in an external instrument with a remote connection to the system e The degasser failed to generate sufficient vacuum for solvent degassing Suggested Actions V Fix the leak in the external instrument before restarting the nano pump V Check external instruments for a shut down condition V Check the vacuum degasser for an error condition Refer to the Reference Manual for the Agilent 1100 Series vacuum degasser 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Remote Time out A not ready condition is still present on the remote input When an analysis is started the system expects all not ready conditions e g a not ready condition during detector balance to switch to run conditions within one minute of starting the analysis If a not ready condition is stil
104. efined time the error message is generated Probable Causes e Disconnected or defective encoder cable e Defective pump drive assembly Suggested Actions V Ensure the encoder cable are not damaged or dirty Make sure the cables are connected securely to the CSM board V Exchange the pump drive assembly 1100 Series Nano Pump Service Manual 61 2 62 Troubleshooting and Test Functions Stroke Length Stroke Length Pump channel A B Stroke Length Pump channel B The distance between the lower plunger position and the upper mechanical stop is out of limits nano pump During initialization the nano pump monitors the drive current If the plunger reaches the upper mechanical stop position before expected the motor current increases as the nano pump attempts to drive the plunger beyond the mechanical stop This current increase causes the error message to be generated Probable Causes e Defective pump drive assembly Suggested Actions V Exchange the pump drive assembly 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Initialization Failed Initialization Failed Pump channel A B Initialization Failed Pump channel B The nano pump failed to initialize successfully within the maximum time window A maximum time is assigned for the complete pump initialization cycle If the time is exceeded before initialization is complete the error message is generated Probable Causes
105. ensation sensor NTC on the CSM board is dependent on ambient temperature The change in resistance is used by the leak circuit to compensate for ambient temperature changes If the resistance across the sensor falls below the lower limit the error message is generated Probable Causes e Defective CSM board Suggested Actions V Exchange the CSM board 1100 Series Nano Pump Service Manual 35 2 Troubleshooting and Test Functions Fan Failed The cooling fan in the nano pump has failed The hall sensor on the fan shaft is used by the CSM board to monitor the fan speed If the fan speed falls below 2 revolutions second for longer than 5 seconds the error message is generated Probable Causes e Fan cable disconnected Defective fan e Defective CSM board e Improperly positioned cables or wires obstructing fan blades Suggested Actions V Ensure the fan is connected correctly V Exchange fan V Exchange the CSM board V Ensure the fan is not mechanically blocked 36 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Open Cover The top foam has been removed The sensor on the CSM board detects when the top foam is in place If the foam is removed the fan is switched off and the error message is generated Probable Causes e The top foam was removed during operation e Foam not activating the sensor e Sensor defective e Rear of the module is exposed to strong direct sunlight Sugges
106. enter the TYPE command correctly 2 The reply line will respond with RA 0000 Type G2226A 3 Turn the nano pump off then on again Then re boot the ChemStation Boot up and subsequent control of the system should be normal 4 The TYPE of a module can also be identified by typing the following command into the command line print sendmoduleS lpmp TYPE The reply line will give the module TYPE 1100 Series Nano Pump Service M anual Repairing the Pump 3 Entering the Serial Number Entering the Serial Number using the Control M odule 1 Connect the control module to the nano pump Turn on the pump 2 In the control module press Views F5 and select the system screen then press Records F4 Using the up down arrows make sure that the nano pump is highlighted 3 Press FW Update F5 Now press the m key This will display a box which says Update Enter Serial 4 Press Enter This will display the box labeled Serial 5 Letters and numbers are created using the up and down arrows Into the box labeled Serial enter the 10 character serial number for the nano pump When the 10 character serial number is entered press Enter to highlight the complete serial number Then press Done F6 For firmware revisions below A02 00 it is very important never to press Done if the Serial box is blank In this case the module can no longer be recognized by either the control module or the ChemStation The main board must then
107. est on the Test screen to perform a leak test Several steps like purging the system setting up Isopropanol as solvent on the channel that is supposed to be tested and blocking the purge valve outlet with a blank nut have to be performed before operating the leak test For details use the i key to achieve context sensitive help follow the instructions on the screen and refer to Running the Leak Test on page 74 For evaluating the leak test refer to Evaluating the Results of the Leak Test on page 77 1100 Series Nano Pump Service Manual 255 6 256 Control Module Screens for the Nano Pump Pressure Test uuc 0 00 RGIS Plateau 2 Pkateau 4 30 seconds Plateau 3 30 seconds afebe sacs Plateau 4 kams 30 seconds 30 seconds Use the F3 key Pressure Test to perform a pressure test of the system Several steps like purging the system setting up Isopropanol as solvent on channel A or A2 if solvent selection valve is installed and blocking the column outlet with a blank nut have to be performed before operating the pressure test For details use the i key to achieve context sensitive help follow the instructions on the screen and refer to Running the Normal Mode Pressure Test on page 69 For evaluating the pressure test refer to Evaluating the Results on page 71 Ready uu 2 08mi Execut m E Print 1100 Series Nano Pump Service M anual Agi
108. est Functions 2 System Pressure Abnormally Low The current system pressure is significantly below the typical system pressure produced by this method with this column Table6 System Pressure Abnormally Low Possible causes and actions Possible Causes Suggested Actions Notes Leaks somewhere in the Usea flashlight and absorbent tissue to search At very low flow rates a leak may system for leaks throughout the system never accumulate enough liquid to trigger the module leak sensors Low flow rate leaks are also very hard to see Solvent channels are not e Perform a 4 minute purge at 2500 ul min for This is especially likely if the system correctly purged Ripple each solvent channel has been unused for more than one might also be too high day Dirty solvent inlet filters e Clean or replace the solvent inlet filters To minimize this problem prefilter Solvent intake is being the mobile phase and take restricted Ripple might precautions against algae formation also be too high in the water 1100 Series Nano Pump Service Manual 93 2 Troubleshooting and Test Functions System Pressure Abnormally High The current system pressure is significantly above the typical system pressure produced by this method with this column Table 7 System Pressure Abnormally High Possible causes and actions Possible Causes Suggested Actions Notes The analytical column has become Replace the column Otherwise backflush the plugged c
109. exagonal key Parts Plunger 5063 6586 1 Disassemble the pump head assembly see Removing and Disassembling the Pump Head Assembly on page 120 2 Check the plunger surface and remove any deposits or layers Cleaning can be done with alcohol or tooth paste Replace plunger if scratched Co Plunger surface 3 Reassemble the pump head assembly see Reassembling the Pump Head Assembly on page 127 1100 Series Nano Pump Service Manual 125 3 Repairing the Pump Exchanging the Flow Sensor Frequency Leak on the flow sensor Unstable column flow Flow sensor blocked Tools required 8710 2412 2 5 mm hex key 8710 1534 4 mm open wrench Parts required G1376 60004 Nanoflow sensor y HD UU FP WwW N FE G2226 67300 Flow sensor accuracy calibration capillary 8000 mm 25 um G2226 67300 Turn off the pump Disconnect the EMPV to flow sensor capillary on the flow sensor inlet Disconnect the flow sensor to sampler capillary on the flow sensor outlet Unscrew the flow sensor Install the new flow sensor Reconnect the EMPV to flow sensor capillary on the flow sensor inlet Connect the flow sensor accuracy calibration capillary G2226 67300 on the flow sensor outlet Run the flow sensor accuracy calibration from the ChemStation Follow the step by step procedure At the end of the procedure disconnect the flow sensor accuracy calibration capillary 10 Reconnect the f
110. flow sensitive flow sensor The measured flow is compared with the user entered column flow setpoint The flow sensor controls the EMPV current causing the EMPV to correctly proportion the column flow The primary flow in excess of the required column flow volume is derivated to the waste Solvent degassing is not done directly in the pump A 4 channel low volume micro vacuum degasser available as a separate module provides degassed solvents to the pump channel inputs Solvent degassing is required for best flow stability and detector stability especially at the low flow rates required to run nano LC applications 1100 Series Nano Pump Service M anual Introduction to the Nano Pump 5 Pump drive Power supply NPM boar Damper Fan Outlet valve Pump head A Flow sensor Active inlet valve 2 Pump head E Leak sensor Solvent selection valve Figure 37 Overview of the Nano Pump Hydraulic Path Overview The nano pump is based on the Agilent 1100 binary pump and performs all the functions necessary for a nano flow solvent delivery system Basically these functions are e Low Pressure Metering and High Pressure Delivery e Solvent Compressibility Compensation e Variable Stroke Volume e Column Flow Measurement and Control Low pressure solvent metering and high pressure solvent delivery are accomplished by two pump channels each capable of delivering a maximum of 2 5 ml min flow at up to 400 bar pressure 110
111. for the special application in use V Exchange the defective pump drive assembly 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Testing your Nano Pump Micro Mode Pressure Test Step 1 Step 2 Description This is a fast test to verify the tightness of a micro system where the pump is operating in the micro mode and no manual purge valve is installed The flow path of the system which is tested for tightness is blocked by a blank nut The pressure is increased up to 380 bar and the remaining flow is measured in the flow sensor while the system is blocked The test begins with the initialization of both pump heads Next pump A begins pumping solvent until a system pressure of 380 bar is reached The pump is operating in the pressure control mode at 380 bar for several minutes The remaining flow in the column flow path between the EMPV and the blank nut is measured M ake absolutely sure that all parts of the flow path that are included in the test are thoroughly flushed with IPA before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path will definitely cause the test to fail Running the Test 1 Select the ChemStation s Diagnosis screen from the nano pump tests selection box select Micro Mode Pressure Test 2 Start the test and follow the online instructions In step 10 of following procedure if you block the flow sensor outlet
112. ge Valve on page 114 The slopes and plateaus are evaluated automatically Evaluating the Results of the Leak Test on page 77 describes the evaluation and interpretation of the leak test results Running the test from the ChemStation 1 Select the leak test from the test selection box in the Diagnosis screen 2 Start the test and follow the instructions Leak Test Manual Purge Valve OF x Start Explain Close Result Status Expected total time approx 15 min es y y YOA Test Procedure o ooo ooo ooo J LJ 1 Install and connect manual purge valve o Cd 2 Connect bottle with isopropanol to channel A 2 and BID Cd 3 Open purge valve Oo o 4 Start flushing channel A B for about 2 min Cd 5 Close purge valve CCC o O 6 Connect restriction cap 61313 87305 to valve outlet Z Jo o o y O 7 Start flushing both channels with pressure about 3 min foo o o o o o 8 Open and close purge valve to release system pressure 9 Replace restriction cap with blank nut 01080 83202 110 Open purge valve for pump initialization 11 Initializing pump o ooo y y 11 Initializing pump sl 2 Close purge valve ooo o o T 13 Running leak test method fabow45 mn 14 Evaustingleaktestess OOO o U U i 15_Open purge valve torelease prese __ 1100 Series Nano Pump Service Manual 75 2 Troubleshooting and Test Functions Running the test from the Control M odule 1 2 Place two bottles
113. gnition without cover 37 missing pressure reading 44 motor drive 207 motor drive power 50 non operating altitude 4 non operating temperature 4 not ready condition 24 0 onboard battery 208 operating altitude 4 Operating temperature 4 optimum performance 7 outlet ball valve 108 112 overview pump 195 1100 Series Nano Pump Service Manual P parts control module 173 cover 167 EM PV parts 165 flow sensor 166 foam 171 hydraulic path 161 light pipes 167 pump head 163 pump housing 158 sheet metal kit 170 parts identification cables LAN cables 191 parts main assemblies 158 performance specification 258 pH range 258 physical specification 3 piston 108 125 power consideration 2 Power consumption 4 power consumption 4 power light pipe 167 power supply 147 description 228 specifications 229 power supply indicator 23 power input socket 2 prerun condition 24 pressure above upper limit 40 pressure below lower limit 41 Pressure Converter 208 pressure plot leak test 77 pressure pulsation 200 pressure sensor readings 14 pressure test 22 68 pressure operating range 258 priming 17 priming manual 17 PTFE frit 108 114 115 PTFE lubricant 127 pump head assembly 163 pump head missing 58 pump housing parts 158 pump seals 108 122 purge valve 108 114 115 purging pump 18 R radio interference 266 reassembling the pump head 127 recommended pH r
114. gure9 Typical Leak Test Pressure Profile with IPA Ramp1 The test begins with the initialization of both pumps After initialization plungers Al and B1 are both at the top of their stroke Next the nano pump begins pumping solvent with a flow rate of 150 ul min stroke of 100 ul anda composition of 51 A 49 B Both pumps deliver for one complete pump cycle At the end of this step plungers Al and B1 are at the top of their stroke 1100 Series Nano Pump Service Manual 73 2 74 Troubleshooting and Test Functions Ramp 2 Ramp 3 Plateau 1 Ramp 4 Plateau 2 Ramp 5 Plateau 3 Ramp 6 Plateau 4 The nano pump continues pumping solvent with a flow rate of 150 ul min Channel A delivers for one pump cycle first plunger A2 delivers then plunger A1 followed by channel B plunger B2 then plunger B1 both channels with a stroke of 20 ul The pressure increase during this phase should be linear Large leaks or defects will be characterized by an unstable non linear slope Just before the start of the first plateau plunger A2 delivers with a flow rate of 50 ul min for approximately 8 seconds The system pressure should be 240 bar or higher At plateau 1 plunger A2 delivers with a flow rate of 3 ul min for 30 seconds During this time the slope should be horizontal or slightly positive slight pressure increase A negative slope indicates a leak rate greater than 3 l min Plunger B2 delivers 50 ul min for approximately 8
115. ibility compensation for the solvent in use Small stroke volumes will generate less pressure pulsations than higher stroke volumes at same flow rates In addition the frequency of the pressure pulsations will be higher This will decrease the influence of flow pulsations on quantitative results In gradient mode smaller stroke volumes resulting in less flow ripple will improve composition ripple The nano pump uses a processor controlled ball screw system to drive its pistons The normal stroke volume is optimized for the selected flow rate Small flow rates use a small stroke volume while higher flow rates use a higher stroke volume 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 The stroke volume for the pump is set to AUTO mode This means that the stroke is optimized for the flow rate in use A change to larger stroke volumes is possible but not recommended When the pump is in the standard mode the EMPV is fully closed Total main flow up to 2500 ul min is directed to the LC system Column flow measurement control is disabled This mode is for non capillary LC applications In the micro mode the flow sensor measures and controls column flow in the range of 0 1 ul min to 4 ul min Flow measurement is based on the principle of mass flow temperature sensitivity The flow sensor consists of a heated tube with two temperature sensors As the mobile phase passes through the heated tube the temperature characteri
116. in a capillary The zero point is corrected and calibration factors are evaluated Flow Sensor Accuracy Calibration on page 86 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Status Indicators Two status indicators are located on the front of the nano pump The lower left one indicates the power supply status the upper right one indicates the instrument status Status indicator Power supply indicator Figure 7 Location of Status Indicators Power Supply Indicator The power supply indicator is integrated into the main power switch When the indicator is illuminated green the power is ON When the indicator is off the module is turned off Otherwise check power connections availability of power or check functioning of the power supply 1100 Series Nano Pump Service Manual 23 2 Troubleshooting and Test Functions Instrument Status Indicator The instrument status indicator indicates one of four possible instrument conditions When the status indicator is OFF and power switch light is on the nano pump is in a prerun condition and is ready to begin an analysis A green status indicator indicates the nano pump is performing an analysis run mode A yellow indicator indicates a not ready condition The nano pump is ina not ready state when it is waiting for a specific condition to be reached or completed
117. in cover 153 AUTO mode 201 AUX output 14 battery 208 safety information 265 BCD board 129 215 BCD output 202 BCD LAN board 215 bench space 3 blank nut 69 74 block diagram 210 211 board connector 134 board layout 135 boards interface board BCD LAN 215 C cable CAN 14 GPIB 14 overview 174 CAN bus 202 CAN cable 14 CAN interface 219 ChemStation 14 cleaning the pump 106 compensation sensor open 34 compensation sensor short 35 composition precision 258 compressibility compensation 258 condensation 3 configuration switch 223 control module EMF 251 firmware update 251 serial number change of MWD 252 tests 254 control module parts 173 cover parts 167 D damper 141 damping unit 141 degreaser spray 122 delay volume 7 delivery checklist 5 description leak test 73 dimensions 3 disassembling the pump head 120 early maintenance feedback EM F 204 electrical connections 202 electronic fuses 202 208 electronics HPM board 207 electrostatic discharge ESD 105 EMF on control module 251 EMF counter 205 EMF flag 205 EMF limits 206 EM PV cleaning procedure 91 EM PV parts 165 1100 Series Nano Pump Service M anual EMPV test 84 encoder missing 51 environment 2 3 error zero solvent counter 39 error condition 24 error message wait timeout 64 error messages 21 24 compensation sensor open 34 compensation sensor short 35 encoder mi
118. ions 2 EM PV failed to initialize micro mode only An attempt to pump in the micro mode has resulted in either an EMPV Initialization Failed error message or a permanent EMPV Initialization not ready message Make sure the system pressure is higher than 20 bar Table8 EMPV failed to initialize Possible Causes and Suggested Actions Possible Causes Suggested Actions Notes The no flow pressure of the system Set the flow to zero and disconnect the blue This problem typically causes is higher than 10 bar flexible capillary going from the damper to the a permanent EM PV mixer The system pressure reading should be Initialization not ready close to zero bar message If the system pressure reading is higher than 4 bar call Agilent service or refer to the Nano Pump Service M anual The inlet to the EM PV has been blocked or partially restricted The M ake sure the solvent channels are well purged This problem typically causes Check the EM PV filter Perform a pump purge at an EM PV Initialization Failed EM PV cannot take in sufficient 1000 ul min using pure water During the purge error message flow to deliver the correct flow check system pressure If pressure is gt 10 bar output The EM PV initialization replace the filter in front of the EM PV routine cannot be done within the Check the flow path from the damper outlet to the required 2 minute period EM PV inlet for plugs or restrictions Check the EM PV to fl
119. irmware The installation of new firmware is required e if anew version solves problems of the currently installed version e if the version of firmware on the new main board NPM after an exchange of the board is older than the one previously installed To upgrade the nano pump s firmware follow the procedures and instructions given on the internet http lsbu marketing agilent com start start asp To download and install always the newest available version of firmware on your system or call your local service provider for assistance 140 1100 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging the Damper Frequency No pressure output or when leaking Tools required Screwdriver Pozidriv 1 Wrench 1 4 inch Parts required 79835 60005 Damper Do not totally remove the Z panel when the damper is in its place The heavy damper may fall down 1 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 131 2 Disconnect both active inlet valve cables 3 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector 4 Remove the flow sensor For this disconnect first the capillary coming from the EMPV the capillary on the injection device port 1 5 Unclip the mixing chamber from its holder 6 Remove the damper and the Z panel For this disconnect fir
120. l present on the remote line after one minute the error message is generated Probable Causes e Not ready condition in one of the instruments connected to the remote line e Defective remote cable e Defective components in the instrument showing the not ready condition Suggested Actions V Ensure the instrument showing the not ready condition is installed correctly and is set up correctly for analysis V Exchange the remote cable V Check the instrument for defects refer to the instrument s reference documentation 1100 Series Nano Pump Service Manual 29 2 30 Troubleshooting and Test Functions Synchronization Lost During an analysis the internal synchronization or communication between one or more of the modules in the system has failed The system processors continually monitor the system configuration If one or more of the modules is no longer recognized as being connected to the system the error message is generated Probable Causes e CAN cable disconnected e Defective CAN cable e Defective main board in another module Suggested Actions V Ensure all the CAN cables are connected correctly V Switch off the system Restart the system and determine which module or modules are not recognized by the system V Ensure all CAN cables are installed correctly 1100 Series Nano Pump Service M anual Leak Troubleshooting and Test Functions 2 A leak was detected in the nano pump The sign
121. l you receive Views on the F5 key Choose System from the pull down menu Use the F3 key Tests to select the pump _Pump _ Temp O tice 0 008GIC Pagado Wed 16 24 PUMP OFF TEMP OFF 0B Module Message Id Date Time ontroller equence modifie I 05 03 16 19 Controller Module added 1HPLC System 05 03 16 14 44 Cap Pump Setpoint chang INFO 05 03 16 41 51 Cap Pump Timetable chan2 Autosampler NFI 05 03 16 08 39 Cap Pump Timetable chan 4 Col Comp Control Jl configure jt ecm 0 OOM Note ie Calibration Press F1 Calibrate to access the pump s calibration screen Select one of the calibration curves stored on the module with the help of the selection keys 254 1100 Series Nano Pump Service Manual Control Module Screens forthe Nano Pump 6 H20 MeOH H20 2 Prop H20 H20 Water vs Isopropanol Press Enter once you have selected highlighted a calibration curve to edit the individual data points of this curve Editing the name of the curve and acknowledging the changes with F6 Done saves the new data curve with its new name It is possible to store one individually created calibration curve with each method Load Save a calibration curve from to the PCMCIA Card by pressing F8 PC Card Bucy 0 00 RSH ee 0 1 000 10 1 000 20 1 000 20 1 000 40 1 000 50 1 000 60 1 000 70 1 000 s0 1 000 90 1 000 100 1 000 Leak Test Press F2 Leak T
122. le at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 135 7 Remove the pump drive For this disconnect first the connector P8 P21 or P12 P14 at the main board 8 Place the new pump drive into the recess Reconnect the connectors at the main board 9 Re install the Z panel and the damper Reconnect the capillaries and connectors P16 P11 P20 10 Re install the mixing chamber to its holder 11 Re install the pump head Reconnect the capillaries and the cable 12 Re install the flow sensor Reconnect the capillaries and the cable 13 Re install the EMPV Reconnect the capillaries the waste tube and the cable 14 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 154 1100 Series Nano Pump Service M anual Repairing the Pump 3 Exchanging the Power Supply Frequency If defective Tools required Screwdriver Pozidriv 1 Wrench 1 4 inch e Wrench 14mm e Wrench 7mm Wrench5mm Parts required Power supply 0950 2528 1 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 131 2 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector 3 Remove the flow sensor For this disconnect first the capillary coming f
123. lent 1100 Series Nano Pump Service Manual 7 Specifications Performance Specification Agilent 1100 Series Nano Pump 258 Performance Specification Agilent 1100 Series Micro Degasser 259 ot Agilent Technologies 257 7 Performance Specifications 258 Table 63 Performance Specification Agilent 1100 Series Nano Pump Type Specification Hydraulic system Settable column flow range Recommended column flow range Optimum composition range Composition precision Delay volume Pressure range Compressibility compensation Recommended pH range Control and data evaluation Analog output Communications Two dual piston in series with proprietary servo controlled variable stroke drive floating piston active inlet valve solvent selection valve and electronic flow control for flow rates from 0 1 to 1 pl min 0 01 4 ul min 1 2500 ul min with the electronic flow control bypassed 0 1 1 pl min 200 2500 ul min with the electronic flow sensor bypassed 1 to 99 or 5 ul min per channel primary flow whatever is greater lt 0 2 SD at 500 nl min default settings Minimum primary flow pump channel is 5 wl min Typically 300 nl from the electronic flow control to the pump outlet for flow rates up to 4 ul min For flow rates up to 4 ul min and electronic flow control active primary flow path 180 480 ul system pressure dependent default settings calculated volume Typically
124. let tube connection should point to the right corner of the pump head Reconnect the inlet tube and the active inlet valve cable to the connector at the Z panel M ake sure you are in normal mode 5 110 After an exchange of the valve cartridge it may take several mL of pumping with the solvent used in the current application before the flow stabilizes at A ripple as low as it used to be when the system was still working properly 1100 Series Nano Pump Service M anual Repairing the Pump 3 a Solvent inlet tube Active inlet valve Plug Figure 15 Exchanging the Active Inlet Valve 1100 Series Nano Pump Service Manual 111 3 Repairing the Pump Exchanging the Outlet Ball Valve Sieve or the Complete Valve 112 Frequency Sieve whenever the pump seals will be exchanged Valve if internally leaking Tools required Wrench 1 4 inch Wrench 14 mm Parts required Outlet ball valve G1312 60008 Sieve pack of 10 5063 6505 Before exchanging the outlet ball valve you can try to clean it in a sonic bath Remove the gold seal and the sieve Place the valve in upright position onto the plastic cap in a small beaker with alcohol Place in a sonic bath for 5 to 10 minutes Insert a new sieve and replace the gold seal 1 Using a 1 4 inch wrench disconnect the valve capillary from the outlet ball valve 2 Using the 14 mm wrench loosen the valve and remove it from the pump body
125. lide the leak sensor cable through the bottom foam and fix the leak sensor cable in the foam Make sure the leak sensor cable is not damaged by the sheet metal 15 Re install the CSM board Reconnect all the cables 16 Re install the solvent selection valve 17 Re install the two pump head Reconnect the capillaries and the cable 18 Re install the Z panel and the damper Reconnect the capillaries and connectors P16 P11 P20 19 Re install the mixing chamber to its holder 20 Re install the flow sensor Reconnect the capillaries and the cable 21 Re install the EMPV Reconnect the capillaries the waste tube and the cable 22 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 154 1100 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging the Leak Sensor Frequency Leak messages without leak in the funnel Tools required e Screwdriver Pozidriv 1 Wrench 1 4 inch Wrench 14mm Wrench 7 mm Wrench 5 mm Parts required Leak sensor 5061 3356 1 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 131 2 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector 3 Remove the flow sensor For this disconnect first the capillary coming from the EMPV the
126. limit exceeded 49 temperature out of range 48 tests on control module 254 timeout 27 top cover 131 154 tweezers 115 U unpacking the pump 5 V variable stroke volume 200 voltage range 3 202 voltage selector 202 W wait timeout 64 wall socket 2 wash seals 108 126 weight 3 wideranging capability 2 wrench 1 4 inch 69 74 112 114 115 117 120 141 143 145 147 149 273 Index wrench 14 mm 109 112 114 115 117 134 149 wrench 5 mm 134 149 wrench 7 mm 134 149 Z zero solvent counter 39 274 1100 Series Nano Pump Service Manual l 2 Agilent Technologies In This Book This manual contains technical reference information about the Agilent 1100 Series Nano pump The manual describes the following e installation e optimizing performance e diagnostics and troubleshooting e repairing e parts and materials e introduction to the pump theory of operation e screens of the local control module and e specifications G2226 90100
127. low sensor to sampler capillary on the flow sensor outlet 126 1100 Series Nano Pump Service M anual Repairing the Pump 3 Reassembling the Pump Head Assembly Tools required 3 mm hexagonal key 4 mm hexagonal key PTFE lubricant 79841 65501 1 Place the support rings on the plunger housing plungers not installed and snap the pump head and plunger housing together Support ring r plunger housing 2 Tighten the lock screw Lock screw 3 Carefully insert the plungers into the pump head assembly and press them completely into the seals ee a 1100 Series Nano Pump Service Manual 127 3 Repairing the Pump Apply a small amount of pump head grease to the Tighten screws stepwise with increasing torque Balls of spindle drive Pump head screws 4 Slide the pump head assembly onto the pump drive pumphead screws and the balls of the spindle drive 5 Reconnect the capillaries tubing and the active inlet valve cable to the connector AIV connector capillary Inlet tube 128 1100 Series Nano Pump Service M anual Repairing the Pump 3 Exchanging the Optional Interface Board The interface board is sensitive to electrostatic discharge Always use the ESD kit when handling electronic boards When required Board defective Part required BCD Interface board see Optional Interface Boards on page 215 1 Switch off the
128. m line unplug the power cord The power supply still uses some power even if the power switch on the front panel is turned off 14 1100 Series Nano Pump Service Manual Installing the Pump 1 Install the Degasser G1379A CAUTION Ensure the line power switch at the front of the degasser is off Place the degasser on top of the pump Make sure that the two modules are interlocked correctly Connect one end of the remote cable 5061 3378 to the rear of the degasser Connect the other end of the cable to the remote port at the rear of the pump The degasser accessory kit has a set of 4 solvent tubes G1322 67300 Each tube is labeled A B C or D Connect each solvent tube to its intended OUTLET channel port on the degasser Connect the other end of the solvent tube to its intended port at the pump solvent selection valve Follow the guide below Degasser Pump Solvent Selection OUTLET Valve Port A to A1 left half upper B to A2 left half lower C to B1 right half upper D to B2 right half lower Install the Solvent Cabinet 1 The solvent cabinet accessory kit has 4 bottle head assemblies G1311 60003 Connect a bottle head assembly to each of the degasser INLET ports Use the labels provided with each bottle head assembly to appropriately label each bottle head assembly Purge your system before first use see Get the System Ready for the First Injection on page 17 1100 Series Nano Pu
129. mp Service Manual 15 1 Installing the Pump ww 6 1 fi Figure6 Flow connection of the capillary pump Table 3 Connecting capillaries for the Nano Pump Item Part Number 1 G1311 67304 2 61312 67300 3 61312 67302 4 61312 67304 5 01090 87308 6 G1375 87400 7 220 mm 25 um G1375 87321 8 350 mm 25 um G1375 87322 8 550 mm 25 um G1375 87323 16 1100 Series Nano Pump Service Manual Installing the Pump 1 Get the System Ready for the First Injection When you are using the system for the first time after installation best results are obtained by performing the following 3 step system preparation in the order given below 1 Manually priming the solvent channels 2 Purging the pump When opening capillary or tube fittings solvents may leak Please observe appropriate safety precautions such as eye protection safety gloves protective clothing as described in the material handling information and safety data sheet supplied by the solvent vendor especially when hazardous solvents are used M anually Priming the Solvent Channels This procedure should be done before the modules are turned on 1 The degasser accessory kit contains a 20ml plastic syringe and a solvent tube adapter for this syringe Push the adapter onto the syringe 2 Pour the intended analytical solvents into the solvent bottles and install the bottles on the desired solvent channels Install Isopropanol on channels which will not be used
130. n the line must be pumped from channel A the right one must be connected to channel B Changes must be activated with F6 Done Line I Loc MG Inj 4 A Time HEMT Abort Ready BMN OFF A 100 100 Caibrated as A Aqueous B Aqueous uncalibrated Comp Change Select Channel Selection to choose one of the A channels 1 or 2 and one of the B channels 1 or 2 as the sources for solvent delivery Changes must be activated with F6 Done 1100 Series Nano Pump Service Manual 237 6 Control Module Screens for the Nano Pump A ca Channel Selection Settings More Select Auxiliary to access the pumps performance optimization parameters Compressibility and Minimum Strike for each channel individually Changes must be activated with F6 Done Danrwu nouuy Channel amp Ghamel B Compressibility EI 10 bar Minimum Stroke 50 pl 238 1100 Series Nano Pump Service M anual Control Module Screens forthe Nano Pump 6 The following screen is only available in Micro Flow mode see below Select Primary Flow to choose one of the three possible ranges for the primary flow Changes must be activated with F6 Done Settings MA EA ET oreo oo as are 0 8 1 3 ml min 3 0 5 0 8 ml min Low Solvent 0 2 0 5 ml min Consumption Select Mode to toggle between Micro Flow and Normal Flow Changes must be activated with F6 Done Danes mean y
131. nano pump should now be displayed 4 While in the Tests screen press m m m dot m From the box now displayed select the Command line and press Enter 5 Into the box labeled Instr enter the command TYPE G2226A 1100 Series Nano Pump Service Manual 137 3 138 Repairing the Pump Letters and numbers are created using the up and down arrows There must be a blank space between the word TYPE and the letter G It is important to enter the TYPE command correctly An incorrect type command may cause the module to turn on in it s resident mode In such a case re enter the TYPE command correctly 6 When the command is entered press Enter to highlight the complete command 7 Press the Execute F8 key Below the box a reply line should then say Reply RA 0000 TYPE G2226A 8 Turn off the module then turn it on again Turn on should be normal In the Records screen the product column should indicate the nano pump If a ChemStation is also connected re boot it now Entering the Type Command using the ChemStation The TYPE is entered by typing a specific command into the command line at the bottom of the main user interface screen 1 To enter the TYPE for a specific module type the following command into the command line print sendmoduleS lpmp TYPE G2226A It is important to enter the TYPE command correctly An incorrect type command may cause the module to turn on in it s resident mode In such a case re
132. nded Cables For point to point connection not using a network hub use a twisted pair cross over LAN cable P N 5183 4649 10 feet long For standard network connections using a hub use category 5 UTP cables P N G1530 61480 8 m long 1100 Series Nano Pump Service Manual 191 4 Parts and Materials 192 1100 Series Nano Pump Service Manual Agilent 1100 Series Nano Pump Service Manual 5 Introduction to the Nano Pump Introduction to the Nano Pump 194 Electrical Connections 202 Instrument Layout 204 Early Maintenance Feedback EMF 205 The Electronics 207 Firmware Description 213 Optional Interface Boards 215 Interfaces 218 Setting the 8 bit Configuration Switch 223 The Main Power Supply Assembly 228 ot Agilent Technologies 193 5 Introduction to the Nano Pump Introduction to the Nano Pump 194 The nano pump consists of two identical pumping units in a single housing It generates gradients by high pressure mixing A solvent selection valve provides flexibility in the choice of solvents Mobile phase composition is produced by mixing the outputs of pump A and pump B The solvent selection valve allows the pump A output to originate from either channel Al or channel A2 The pump B output may originate from either channel B1 or channel B2 The primary flow produced by the two pumping units is proportioned in an electomagnetical proportional valve EMPV The remaining column flow is measured in a mass
133. nector Probable Causes e Damper disconnected e Defective damper Suggested Actions V Ensure the damper is connected correctly to the CSM board V Exchange the damper 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Valve Failed Valve 0 Failed valve Al Valve 1 Failed valve A2 Valve 2 Failed valve B2 Valve 3 Failed valve B1 One of the solvent selection valves in the nano pump failed to switch correctly The processor monitors the valve voltage before and after each switching cycle If the voltages are outside expected limits the error message is generated Probable Causes e Solvent selection valve disconnected e Connection cable inside instrument not connected e Connection cable inside instrument defective e Solvent selection valve defective Suggested Actions V Ensure the solvent selection valve is connected correctly V Ensure the connection cable is connected correctly V Exchange the connection cable V Exchange the solvent selection valve 1100 Series Nano Pump Service Manual 43 2 44 Troubleshooting and Test Functions Missing Pressure Reading The pressure readings read by the pump ADC analog digital converter are missing The ADC reads the pressure readings from the damper every 1ms If the readings are missing for longer than 10 seconds the error message is generated Probable Causes e Damper not connected e Defective damper e Defective CS
134. nual 107 3 Repairing the Pump Simple Repair Procedures The procedures described in this section can be done with the nano pump in place in the system stack Table 14 Simple Repair Procedures Procedure Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve on page 109 Exchanging the Outlet Ball Valve Sieve or the Complete Valve on page 112 Installing the M anual Purge Valve on page 114 Installing the M anual Purge Valve on page 114 Exchanging the Pump Seals and Seal Wear in Procedure on page 122 Exchanging the Plungers on page 125 Exchanging the Flow Sensor on page 126 108 Symptom If internally leaking If internally leaking Unstable column flow or system pressure Column flow and system pressure drops from time to time If pump performance indicates seal wear If scratched Extended flow range 100 ul needed Leak on the flow sensor Unstable column flow Flow sensor blocked Notes Pressure ripple unstable run leak test for verification Pressure ripple unstable run leak test for verification A pressure drop of gt 10 bar across the frit 2 5 ml min H20 with purge open indicates blockage Leaks at lower pump head side unstable retention times pressure ripple unstable run leak test for verification Seal life time shorter than normally expected check plungers while changing the seals 1100 Series Nano Pump
135. nut immediately before the suspected component then run the pressure test again If the test passes the defective component is located after the blank nut Confirm the diagnosis by placing the blank nut immediately after the suspected component The diagnosis is confirmed if the test fails Running the Normal M ode Pressure Test Tools required Wrench 1 4 inch Parts and materials Blank nut 01080 83202 required Isopropanol 500 ml This test requires the manual purge valve installed and the Electronic Flow Control EM PV and flow sensor bypassed To install the manual purge valve see Installing the M anual Purge Valve on page 114 M ake absolutely sure that all parts of the flow path that are part of the test are thoroughly flushed with IPA before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path definitely will cause the test to fail Running the test from the ChemStation 1 Select the pressure test from the test selection box in the Diagnosis screen 2 Start the test and follow the instructions 1100 Series Nano Pump Service Manual 69 2 70 Troubleshooting and Test Functions Normal Mode Pressure Test OF x Start Explain Close Result Status o l Block system with blank nut 01080 83202 5 Start flushing channel for about 2 min 7 Running pressure test about 4 5 min 8 Evaluating pressure test 9 Open purge valve to release pressure 6
136. o Pump Service Manual vii viii Exchanging the Active Inlet Valve Cartridge or the Active Inlet Valve 109 Removing the Active Inlet Valve 109 Exchanging the Valve Cartridge 109 Replacing the Active Inlet Valve 110 Exchanging the Outlet Ball Valve Sieve or the Complete Valve 112 Installing the Manual Purge Valve 114 Installing the Purge Valve 114 Exchanging the Purge Valve Frit or the Complete M anual Purge Valve 115 Exchanging the EM PV Assembly 117 Exchanging the Solvent Selection Valve 118 Removing and Disassembling the Pump Head Assembly 120 Exchanging the Pump Seals and Seal Wear in Procedure 122 Exchanging the Plungers 125 Exchanging the Flow Sensor 126 Reassembling the Pump Head Assembly 127 Exchanging the Optional Interface Board 129 Exchanging Internal Parts 130 Removing the Top CoverandFoam 131 Exchanging the Nano Pump Main Board NPM Board 134 Entering the Type Command 137 Entering the SerialNumber 139 Replacing the Nano Pump s Firmware 140 Exchanging the Damper 141 Exchanging the Fan 143 Exchanging a Pump Drive 145 Exchanging the Power Supply 147 1100 Series Nano Pump Service Manual Exchanging the Leak Sensor 149 Exchanging Status Light Pipe 152 Assembling the Main Cover 153 Replacing the Top Cover and Foam 154 4 Parts and Materials Nano Pump M ain Assemblies 158 Solvent Cabinet and Bottle Head Assembly 160 Nano Pump Hydraulic Path 161 Pump Head Assembly 163 Electro M agnetic Proportional Valve E
137. o hydrostatic pressure 3 Using the 14 mm wrench unscrew the purge valve and remove it from the purge valve holder 4 Remove the plastic cap with the gold seal from the purge valve 5 Using a pair of tweezers or a toothpick to remove the frit Valve body Uf An PEt A eee OY Plastic cap Figure 19 Purge Valve Parts 6 Place a new frit into the purge valve with the orientation of the frit as shown above 7 Replace the cap with the gold seal Before reinstallation always check the gold seal A deformed seal should be exchanged 8 Insert the purge valve into the purge valve holder and locate the pump outlet and the waste outlet as shown below 9 Tighten the purge valve reconnect outlet capillary and waste tubing When fused silica capillaries are used use the 4 mm wrench together with the torque tool to avoid overtightening 1100 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging the EM PV Assembly Frequency If valve internally leaking If unstable column flow in micro mode If column flow and system pressure drops from time to time Tools required Wrench 1 4 inch Wrench 14mm Wrench 7 16 inch Parts required EM PV assembly G1361 60000 Exchanging the complete EM PV 1 2 3 Turn the pump off Remove the pump from the stack and remove the top cover and foam Using a 1 4 inch wrench disconnect the capillaries going to the flow sensor coming from the filter Disconnec
138. o total volume By selecting the respective tick boxes you can also choose whether and when to have Not Ready or Error conditions Changes must be activated with F6 Done Refill With the F5 key Runtimes you can change the stop time and the post run time for this module individually Changes must be activated with F6 Done Danes y ee ee Stoptime M 0 00 min Posttime 0 00 min overrides HPLC system runtime settings Press F5 key Views and select Status 1100 Series Nano Pump Service Manual 241 6 Control Module Screens for the Nano Pump _Line Ha _ a Time a amn eee Wed 16 1218 1 Status This is an example if an Agilent 1100 pump is configured standalone Information on the actual flow rate mobile phase composition pressure and Ripple elapsed run time and the pressure plot are shown Press key F8 Start to start a run key F7 Rescale to maximize the signal we Eo Time BORT ide Ready E Flow 1 0171 umin 5 s OFF Pressure 1bar Ripple 0 0 Elapsed 0 00 min Signal plot Press F6 key Plot to enter the plot screen available also from all other views Here you can observe online signal s If more than one signal is configured use the 1 2 3 numeric keys to switch between the signals OMNES 286 LEa Miu 0 008 Ready 285 4 bar 0 4 fA but Select 242 1100 Series Nano Pump Service Manual Control Module Screens forthe Nano Pump 6
139. oard If excessive current is being drawn for long periods the temperature of the circuits increase If the temperature exceeds the upper limit of 95 C the error message is generated Probable Causes e High friction partial mechanical blockage in the pump drive assembly e Partial blockage of the flowpath in front of the damper e Defective drive assembly e Defective CSM board Suggested Actions V Ensure the capillaries and frits between the pump head and damper inlet are free from blockage V Ensure the outlet valve is not blocked V Remove the pump head assembly Ensure there is no mechanical blockage of the pump head assembly or pump drive assembly V Exchange defective drive assembly V Exchange the CSM board 1100 Series Nano Pump Service Manual 49 2 50 Troubleshooting and Test Functions M otor Drive Power Motor Drive Power Pump channel A B Motor Drive Power Pump channel B The current drawn by the pump motor exceeded the maximum limit Blockages in the flowpath are usually detected by the pressure sensor in the damper which result in the nano pump switching off when the upper pressure limit is exceeded If a blockage occurs before the damper i e the pressure increase cannot be detected by the pressure sensor the nano pump will continue to pump As pressure increases the pump drive draws more current When the current reaches the maximum limit the nano pump is switched off and the error messag
140. of LC grade isopropyl alcohol in channels A2 and B2 Set flow to 2500 ul min for channel A2 and B2 and flush the degasser for about 2 minutes If the pumps seals were replaced or the seals are not sufficient settled use the following procedure 76 Connect the restriction capillary G1313 87305 to flow sensor outlet Set flow to 2500 ul min normal mode and 50 B2 Pump for about 10 min Stop the flow Set flow to 0 ul min and replace the restriction capillary with blank nut 01080 83202 Connect the signal cable to the analog output at the rear of the nano pump only if an integrator is used Press Execute to initialize the leak test Once the test is started the nano pump increase the pressure and run each plunger at low flow rate The control module displays a graphical representation of the pressure in the plateau windows Evaluating the Results of the Leak Test on page 77 describes the evaluation and interpretation of the leak test results When the test is finished slowly open the blank nut to release the pressure in the system 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Evaluating the Results of the Leak Test Defective or leaky components in the pump head lead to changes in the leak test pressure plot Typical failure modes are described below Pressure bar aoa Plateau3 Plateau 4 P Plateau 1 Plateau 2 Time minutes Figure 10 Leak Test Pressure Plot Pl
141. olumn or replace the column inlet frit The filter in front upstream ofthe Perform a pump purge at 1000 ul min using pure EM PV has become plugged water During the purge check system pressure If pressure is gt 10 bar replace the EM PV filter Acomponentinthe micro sampler Using the sampler maintenance positions switch For severe capillary tube has become plugged This could be the sampler injection valve from mainpass to plugs acetone is a good the sample loop needle needle bypass If pressure is significantly reduced backflushing solvent seat assembly or injection valve Backflush or change the needle seat assembly ports Replace the needle Backflush or replace the sample loop capillary Replace the injection valve rotor seal e Clean the stator head with acetone and make sure the stator head ports are free of particles A capillary before or after the e Refer to the system flow diagram One at a time sampler in the system has become disconnect the capillaries in the following order plugged broken crushed by a When the defective capillary is found it may be module cover or overtightened backflushed with acetone or replaced EMPV to flow sensor capillary e flow sensor to sampler injection valve capillary sampler injection valve to column inlet capillary flow cell assembly includes inlet and outlet capillaries 94 1100 Series Nano Pump Service Manual Troubleshooting and Test Funct
142. om solvent bottle From solvent bottle EMPV Flow sensor Damper Filter To waste Figure 38 The Hydraulic Path How Does the Pumping unit Work Both pumping units channel A and channel B are identical with respect to parts and function Each pumping unit consists of a pump head which is directly attached to a metering drive assembly In each metering drive assembly a servo controlled variable reluctance motor and gear train assembly are used to move two ball screw drives The gear train moves the two ball screw drives in opposite directions 180 degree out of phase The gear ratios are designed such that the first ball screw drive constantly moves at twice the speed of the second ball screw drive The servo motor includes a high resolution shaft position encoder which continuously reports the speed and direction of the motor in real time This speed and direction information is used by the pump control electronics to ensure precise control of the servo motor movement 1100 Series Nano Pump Service Manual 197 5 198 Introduction to the Nano Pump Each pump head consists of two identical chambers pistons and seals plus an active inlet valve and an outlet ball valve The solvent volume in each chamber is displaced by its piston The pistons are directly moved by the reciprocating ball screw drives of the metering drive assembly Due to the gear design of the metering drive assembly the pistons move in opposite direction
143. ons only a small part of the available 40ul metering For best results in clearing bubbles 1100 Series Nano Pump Service Manual 99 2 Troubleshooting and Test Functions Wandering Detector Baseline Critical Decision Determine if the problem is in the DAD or coming from the LC system Remove the flow cell from the DAD Close the cell cover and see if the baseline performance improves 1 If baseline performance has not improved a Replace the lamp s b Evaluate the environment for excessive drafts temperature changes etc 2 If baseline performance has improved focus attention on the possible causes and suggested actions below Table 12 Wandering Detector Baseline Possible Causes and Suggested Actions Possible Causes Suggested Actions Notes Dirty or defective flow cell e Clean or replace the flow cell The analytical column Bypass the column Pump directly into the flow cell If performance improves try a new column Mixing Noise when Try premixing the mobile phase in one bottle This problem occurs when one or pumping a binary mobile and pumping 100 from that one solvent both of the solvents has a high phase from two channels channel background absorbance at the If baseline performance improves a solution detection wavelength In this case must be found which is a compromise between the pump s mixing efficiency may mixing volume and other chromatographic not be good enough to produce a requirements F
144. op over the inline filter is measured The pump is operating in the flow control mode and is delivering 2 ul min for approximately 3 minutes At the end of this step the pump is switched to pressure control mode The actual pressure is the initial pressure The pump is delivering solvent for 3 minutes at a pressure equal to the initial pressure divided by 2 At the end of this step the corresponding flow sensor response is measured The pump is delivering solvent for 3 minutes at a pressure equal to the initial pressure divided by 4 At the end of this step the corresponding flow sensor response is measured The pump is delivering solvent for 2 minutes at a pressure equal to the initial pressure divided by 10 At the end of this step the corresponding flow sensor response is measured The pump is delivering solvent for 2 minutes at a pressure equal to the initial pressure divided by 20 At the end of this step the corresponding flow sensor response is measured The EMPV is opening to release the pressure No primary flow is delivered After 2 minutes the flow sensor offset and pressure offset are measured 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 M ake absolutely sure that all parts of the flow path that are included in the test are very thoroughly flushed with WATER before starting to pressurize the system Any trace of other solvents or the smallest air bubble inside the flow path will definitely cau
145. or an isocratic analysis sufficiently homogeneous mobile premixing and pumping 100 one channelis the phase best solution The detector reacts to gluts of the more detectable parts of the solvent mixture and baseline disturbances result Unstable flow and or system Refer to the problem Unstable flow and or Failure to maintain stable column pressure system pressure above If yoursystem suffers flow or system pressure can also from this problem go through the possible cause unwanted baseline activity causes and suggested actions described there 100 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 User interface displays error messages for specific modules Table 13 User interface displays error messages for specific modules Possible causes and suggested actions Possible Causes Suggested Actions Notes A module has experienceda Referto the Reference M anual supplied with A specific error message for that specific hardware failure the module Follow the advice on module is displayed during operation troubleshooting and repair forthe errormessage The status indicator of that module displayed is red 1100 Series Nano Pump Service Manual 101 2 Troubleshooting and Test Functions 102 1100 Series Nano Pump Service Manual Agilent 1100 Series Nano Pump Service Manual 3 Repairing the Pump Introduction 105 Simple Replacements 105 Exchanging Internal Parts 105 Cleaning the Nano
146. other parts also continue with that work first 8 On the new board check the switch setting of address switch S1 see Table 51 on page 219 An incorrect switch setting e g TEST BOOT may cause the pump to turn into a basic mode yellow or red flashing status light In such a case turn off the pump re set the address switches and turn on the pump again 9 Install the new board and reconnect the connectors Make sure that the board is fitted correctly in the board recess holes at the rear panel CAUTION Make sure that P21 is not accidentally connected into the position of P16 P17 possible on revision A and B boards This will damage the encoder of pump drive B when turned on 10 Replace the connector screws 5 amp mamas SS sSoa5 Ff SS S35 S55S55 SS Se it r ea Nut and screws aaa Coir Bend 2 ry V 100 tan7220 240 d 220 He 60180 a a E sae SSeSeSseSs B ets CESCE ot EH A aea C ERT Sa55 85 e5 2 HERI moen Sc Erua 0 SSSSSS MHMN E 9 99 3 SO Figure 23 Rear of Nano Pump 11 Replace foam and top covers see Replacing the Top Cover and Foam on page 154 12 Reinstall the pump in the stack Reconnect all cables Turn on the nano pump If the status indicator turn
147. ous trend between damage or blockage and Step 1 and Step 5 repeat the test If value still out of range accept the offset only at the end of the test f the flow sensor has a negative offset the minimum flow rate the pump can operate is the numeric value of the measured offset For example If offset flow is 0 030 uL min the minimum flow rate the pump can operate is 30 nL min t The offset of the nano flow sensor has a more significant influence on the flow sensor accuracy than the linearity 90 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 EM PV Cleaning Description Depending on the application sometimes particles can be collected in the EMPV valve This fast cleaning routine is designed to remove such particle deposits The routine should always be performed when the EMPV is suspected of being leaky or contaminated with particles Step1 The outlet of the EMPV is plugged with a blank nut After a short flushing routine the EMPV is closed and the pressure is increased to approximately 380 bar Step2 The EMPV is then opened and the pressure is released very quickly Step3 This procedure is repeated several times in a sequence Running the Cleaning procedure 1 Select the ChemStation Diagnosis screen from the maintenance selection box select EMPV Cleaning 2 Start the test and follow the online instructions EMPY Cleaning Procedure Iof x Start Explain Close
148. ow sensor capillary fora total plug or partial restriction Replace the capillary or backflush the capillary with acetone Replace the EM PV assembly G1361 60000 Call Agilent service or refer to the Nano Pump Service Manual Exchanging the EM PV Assembly on page 117 1100 Series Nano Pump Service Manual 95 2 Troubleshooting and Test Functions Unstable column flow and or system pressure In the micro mode the pump flow control system is active The flow control system continuously measures the actual value of column flow and maintains the requested column flow despite changes in system restriction If the flow control becomes defective actual column flow hence system pressure will fluctuate If the system offers a changing restriction to the pump actual column flow will fluctuate as the pump tries to maintain flow against the changing restriction Therefore in the micro mode unstable column flow and unstable system pressure usually appear together Table9 Unstable column flow and or system pressure Possible Causes and Suggested Actions Possible Causes Suggested Actions Notes The flow setpointis below the Make sure that the column flow setpoint is recommended minimum above the recommended minimum setpoint Normal mode 100 ul min Micro mode 20 ul flow sensor value 1 ul min Micro mode 100 ul flow sensor 10 ul min The system pressure is e Make sure that there is at least 20 bar pressure insufficient for
149. p 2 Step 3 Description The test is designed to verify the performance of the EMPV The test must always be done when the EMPV valve is exchanged The test should also be done if column flow stability problems occurs micro mode only The EMPYV test is not a substitute for the leak test or pressure test The leak and pressure tests should also be done when leaks within the pump heads might be the problem The test starts with a short flushing sequence and a cleaning procedure for the EMPV Afterwards low and high pressure is controlled by the EMPV and the appropriate current is monitored Finally a linear pressure ramp is performed Running the test 1 Select the ChemStation s Diagnosis screen from the nano pump tests selection box select Micro Mode Pressure Test 2 Start the test and follow the online instructions Nano Pump EMPY Test lolx Start Explain Close Result Status Nano PumpEMPY Test o S S Expected total time approx 15min ooo S O G S TestProcedue oo S o 1 Connect bottle with aqueous A1 and organic sovet B1 f o 2 Release system pressure o 3 Block EMPV outlet with blank nut 01080 83202 Jo o 4 Opening EMPY o S S 5 Start flushing both channels together abot 25min J o 6 Execute EMPV cleaning procedure about 2min _ 7 Closing EMPY o o S 8 Running EMPV test about8 min S 9 Opening EMPV to release pressure S 10 Evaluating EMPV test results PP
150. r pressure use restriction capillary Tighten the active inlet valve in channel A 14mm wrench Do not overtighten Ensure the pump head screws in channel A are tight Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve Exchange the pump seals in channel A Check the plungers for scratches Exchange if scratched Exchange the active inlet valve in channel A 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Fourth plateau negative or unstable and at least one other plateau positive 2504 Pa 150 fo Pra Potential Cause Corrective Action Air in pump chamber of channel B orseals not Flush channel B thoroughly with isopropanol yet seated under pressure restriction capillary Loose active inlet valve in channel B Tighten the active inlet valve in channel B 14mm wrench Do not overtighten Loose pump head screws in channel B Ensure the pump head screws in channel B are tight Loose outlet valve in channel B Ensure the sieve in the outlet valve is installed correctly Tighten the outlet valve Leaking seal or scratched plunger in channel B1 Exchange the pump seals in channel B Check the plungers for scratches Exchange if scratched Defective active inlet valve in channel B Exchange the active inlet valve in channel B 1100 Series Nano Pump Service Manual 83 2 84 Troubleshooting and Test Functions EM PV Test Step 1 Ste
151. reliable flow being developed after the pump control micro mode e Add an additional capillary after the pump if required Leaks somewhere in the Usea flashlight and absorbent tissue to search At very low flow rates a leak may system for leaks throughout the system Check for leaks never accumulate enough liquid to after the pump and inside the pump valves trigger the module leak sensors Low fittings etc flow rate leaks are also very hard to e f operating in the micro mode perform the see micro mode pressure test If operating in the Refer to the Nano Pump Service normal mode perform the normal mode Manual Testing your Nano Pump pressure test on page 65 Normal M ode Pressure Test on page 68 One or more solventchannels Performa 2 minute purge at 2500 ul min for are not correctly purged each solvent channel This is especially likely if the system has been unused for more than one Ripple might also be too day high Dirty solvent inlet filters Temporarily remove solvent inlet filters to see if To minimize this problem prefilter Solvent intake is being they are the cause of the problem If so cleanor the mobile phase and take restricted Ripple might also be too high replace the solvent inlet filters precautions against algae formation in the water 96 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Table9 Unstable column flow and or system pressure
152. ress Transter to pass controi to the alternate system Press Execute to update the modules firmware after the reboot 0 TE If you have not saved your methods please do it before continuing Otherwise they will be overwritten during the update process Q Update module Generic using file 13764401 DLB Do not disconnect the cable or power cycle the module while the update is executing In case the serial number of the module has to be added use the m key to open the menu Enter Serial The serial number becomes active after restart of the module Records isis inte 0 00 NRT Ready Execut Select File 1376 A401 DLB Status Idle l You need to transter the 5 alternate system lt gt before you can update it s Transfe Press Transfer to pass c Orr re wreriiae System Press Execute to update the modules firmware after the reboot 0 Type in the module s Serial as indicated on the front or rear label of the module using the alphanumeric keys Changes must be activated with F6 Done 1100 Series Nano Pump Service M anual Control Module Screens forthe Nano Pump 6 Records Time MGMT Abort Ready Select Ein BE nena Aro Obata Products 1G2 22 GA Serial SPE Bie c restart the module to activate changes gt Uo 1100 Series Nano Pump Service Manual 253 6 Control Module Screens for the Nano Pump Diagnostics and Tests Tests screen Use the Esc key unti
153. ries Nano Pump Service Manual Safety Information A Sound Emission M anufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB e Sound Pressure Lp lt 70 dB A e At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 1100 Series Nano Pump Service Manual 267 A Safety Information Solvent Information 268 Solvents Observe the following recommendations on the use of solvents Brown glass ware can avoid growth of algae Always filter solvents small particles can permanently block the capillaries Avoid the use of the following steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like nitric acid sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls O gt gt 2COCl 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade e
154. rking old half 6 Connect the valve module to its electrical connectors and fix the assembly with the two holding screws 7 Reinstall solvent tubes and the active inlet valve connection tubes 1100 Series Nano Pump Service Manual 119 3 Repairing the Pump Removing and Disassembling the Pump Head Assembly CAUTION Had start the pump when the pump head is removed This may damage the pump When required Exchanging the seals Exchanging the plungers Exchanging seals of the seal wash option Tools required Wrench 1 4 inch 3 mm hexagonal key 4 mm hexagonal key Preparations for this procedure Switch off nano pump at power switch Remove the front cover to have access to the pump mechanics 1 Disconnect the capillary at the pumphead adapter and the tube at the active inlet valve Beware of leaking solvents Disconnect the active inlet valve cable plug Active inlet valve Plug 120 1100 Series Nano Pump Service M anual Repairing the Pump 3 2 Using a 4 mm hexagonal key step wise loosen and 3 Place the pump head on a flat surface Loosen the remove the two pump head screws and remove the lock screw two revolutions While holding the pump head from the pump drive lower half of the assembly carefully pull the pump head away from the plunger housing Pumphead screws 4 Remove the support rings from the plunger housing and lift the housing away from the plungers
155. rom the EMPV the capillary on the injection device port 1 the connector 4 Unclip the mixing chamber from its holder 5 Remove the damper and the Z panel For this disconnect first the mixing capillary the capillary going to the mixer the active inlet valve cable at the Z panel the connector P16 P11 P20 at the main board see Figure 22 on page 135 6 Remove both pump assemblies For this disconnect first the capillary coming from the mixing chamber the connecting tube coming from the solvent selection valve the connector P8 P21 and P12 P14 at the main board 1100 Series Nano Pump Service Manual 147 3 148 Repairing the Pump 7 Disconnect the fan cable P17 at the main board 8 Remove the solvent selection valve See exchanging the solvent selection valve in this chapter 9 Remove the CSM board Disconnect the remaining connectors see exchanging the high pressure pump main board in this chapter 10 Push the leak sensor cable through the recess of the solvent selection valve and lift out the bottom foam 11 Remove the power supply For this loosen and remove the power supply screws at the rear panel unclip the power supply light pipe from the power supply and pull out the coupler 12 Place the new power supply into the instrument and fix it with the two screws at the rear panel 13 Place the coupler onto the switch in the power supply and clip the light pipe back onto the coupler 14 S
156. rsonal injury are present in this instrument Use extreme caution when handling testing and adjusting 1100 Series Nano Pump Service Manual 263 A Safety Information Safety Symbols Table 65 shows safety symbols used on the instrument and in the manuals Table 65 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the AN instruction manual in order to prevent risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected conductor terminal Eye damage may result from directly viewing the light produced by the Xenon flash lamp used in this product Always turn the xenon flash lamp off before removing it G O A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could resultin personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met 264 1100 Series Nano Pump Service M anual Safety Information A Lithium Batterie
157. rts 166 foam 131 154 foam parts 171 frequency range 4 202 frit 114 115 fuse 202 208 fuses BCD board 215 power supply 229 fi 272 G GPIB default addresses 219 interface 219 GPIB cable 14 GPIB connector 202 H hexagonal key 3 mm 120 122 125 127 hexagonal key 4mm 120 122 125 127 145 147 high pressure pump main board HPM 207 HPM board 134 humidity 4 hydraulic path parts 161 hydraulic system 258 index adjustment 60 index limit 59 index missing 61 indicator power supply 23 initialization failed 63 injection first 17 inlet valve fuse 47 inlet valve missing 52 installation pump module 12 instrument status indicator 24 interface board 129 interface board BCD LAN 215 interfaces 208 analog signal output 219 APG remote 220 CAN 219 GPIB 219 overview 218 RS 232C 221 internet 269 introduction to the pump 194 L laboratory 3 laboratory bench 3 lamp instrument status 24 lamp power supply 23 LAN interface board 215 LAN cables 191 LAN interface board 217 leak 31 leak converter 208 leak sensor 149 leak sensor open 32 leak sensor short 33 leak test 22 73 leak test evaluation 77 light pipes 167 line frequency 4 202 line voltage 3 202 liquimeter 123 205 lithium batteries 265 logbook 27 M main assemblies parts 158 main assemblies overview 107 main cover 153 maintenance procedures 205 message i
158. s with piston 1 constantly moving at twice the speed of piston 2 The outer diameter of the piston is smaller than the inner diameter of the chamber allowing solvent to flow in the gap between the piston and the chamber wall The two chambers are connected by the pressure dependent outlet ball valve The position of the solvent selection valve determines which of two solvents will be sucked low pressure through the active inlet valve into chamber 1 during the intake stroke of piston 1 The active inlet valve is electrically opened and closed making its operation more precise at low pressures The stroke volume of piston 1 is between 2 ul and 100 ul depending on flow rate When the nano pump is first turned on the user is prompted to initialize the pump The initialization routine occurring for both pump heads first determines the precise movement limits for both pistons These limits are then stored in the pump controller memory Then both pistons are set to their default initial positions When pumping begins the active inlet valve is opened and piston 1 begins its intake stroke sucking solvent into chamber 1 At the same time piston 2 begins its delivery stroke pumping high pressure the existing solvent in chamber 2 out of the pump head The pressure produced by piston 2 also closes the outlet ball valve preventing any chamber 2 solvent from back streaming into chamber 1 After a predefined piston 1 stroke length the servo motor i
159. s Information Danger of explosion if battery is incorrectly replaced Replace only with the same or equivalent type recommended by the equipment manufacturer Lithium batteries may not be disposed off into the domestic waste Transportation of discharged Lithium batteries through carriers regulated by IATA ICAO ADR RID IM DG is not allowed Discharged Lithium batteries shall be disposed off locally according to national waste disposal regulations for batteries Danish Information Lithiumbatteri Eksplosionsfare ved fejlagtic handtering Udskiftning ma kun ske med batteri af samme fabrikat og type Lever det brugte batteri tilbage til leverandoren Lithiumbatteri Eksplosionsfare Ved udskiftning benyttes kun batteri som anbefalt av apparatfabrikanten Brukt batteri returneres appararleverandoren Bij dit apparaat zijn batterijen geleverd Wanneer deze leeg zijn moet u ze niet weggooien maar inleveren als KCA 1100 Series Nano Pump Service Manual 265 A Safety Information Radio Interference Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with equipment unscreened cables and or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still met within the premises 266 1100 Se
160. s back to your GPIB or RS 232 configuration settings Stay Resident Settings 226 Firmware update procedures may require this mode in case of firmware loading errors 1100 Series Nano Pump Service M anual Introduction to the Nano Pump 5 Switches 1 and 2 do not force storage of this set of parameters in non volatile memory Returning switches 1 and 2 to other positions other than being both up will allow for normal operation If you use the following switch settings and power the instrument up again the instrument firmware stays in the resident part that is it is not operable as a detector It only uses basic functions of the operating system for example for communication Table 61 Stay Resident Settings Mode Select 1 2 3 4 b 6 7 8 TEST BOOT 11 1 0 0 1 0 0 0 To return to normal operation set switches back to your GPIB or RS 232C configuration settings 1100 Series Nano Pump Service M anual 227 5 Introduction to the Nano Pump The Main Power Supply Assembly 228 The main power supply comprises a closed assembly no onsite repair possibility The power supply provides all DC voltages used in the module except for the voltages supplied by the lamp power supply to the deuterium and tungsten lamps in the detectors The line voltage can vary in a range from 100 240 volts AC 10 and needs no manual setting MPS 12V supply gt 36V 2 5 A
161. s red continue with the section Entering 136 1100 Series Nano Pump Service Manual Repairing the Pump 3 the Type Command on page 137 other whise continue with the section Entering the Serial Number on page 139 Entering the Type Command After the installation of a new mainboard the TYPE binary of the module is normally automatically detected The specific TYPE tells the pump how to configure itself during turn on In some cases especially when a defective mainboard is replaced with an older version of mainboard the automatic TYPE detection does not work When the TYPE of the exchange main board does not match the pump a pump configuration error will occur during pump turn on This will cause the pump status indicator to be red In this case the TYPE of the module has to be entered using the procedure described in the following sections You must change the TYPE of the replacement main board to agree with the nano pump G2226A The TYPE can be entered using either the control module or the ChemStation see Entering the Type Command using the Control Module on page 137 or Entering the Type Command using the ChemStation on page 138 Entering the Type Command using the Control Module 1 Connect the control module to the pump 2 From Views press F5 select the System screen then press Tests F3 3 Using the up down arrows make sure that the nano pump is highlighted and press Enter The Tests screen for the
162. s stopped and the active inlet valve is closed The pistons now reverse directions Piston 1 begins its delivery stroke high pressure and piston 2 begins its intake stroke Piston 2 is moving at only half the speed of piston 1 The outlet ball valve is forced open by the pressure generated by piston 1 Piston 1 begins to deliver the volume previously sucked into chamber 1 Because of the 2 1 speed ratio of the pistons half of the solvent flow from chamber 1 is forced out of the pump head continuing into the pump hydraulic path The other half of the flow from chamber 1 simultaneously refills chamber 2 When piston 1 has completed its delivery stroke the pistons reverse direction and the cycle is repeated 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 To mixing chamber Inlet From valve solvent bottle Seal Piston Ball screw drive Gear koma M otor with encod Figure 39 Operating Principle of the pump head Table 47 Nano Pump Details Materials in contact with mobile phase Pump head SST gold sapphire ceramic Active Inlet Valve SST gold sapphire ruby ceramic PTFE Outlet Valve SST gold sapphire ruby tantalum Adapter SST gold EM PV SST ruby sapphire PEEK Flow Sensor SST Damping Unit Gold SST Capillaries Fused Silica 1100 Series Nano Pump Service Manual 199 5 200 Introduction to the Nano Pump For pump specifications
163. s to ensure proper functionality and compliance with safety or EMC regulations 218 1100 Series Nano Pump Service Manual Analog Signal Output Introduction to the Nano Pump 5 The analog signal output can be distributed to a recording device For details refer to the description of the module s main board GPIB Interface A chemstation cannot be connected directly to the well plate sampler by GPIB The GPIB connector is used to connect the module with a computer The address and control switches next to the GPIB connector determine the GPIB address of your module The switches are preset to a default address and recognized by the operating software from Agilent Technologies Table51 Default Addresses Autosampler 28 Pump 22 FLD 23 VWD 24 Agilent 8453A 25 DAD MWD 26 Column Compartment 27 RID Autosampler HP 1050 Pump HP 1050 VWD HP 1050 DAD HP 1050 29 18 10 17 CAN Interface The CAN is an intermodule communication interface It is a 2 wire serial bus system supporting high speed data communication and real time requirement 1100 Series Nano Pump Service M anual 219 5 220 Introduction to the Nano Pump Remote Interface The APG remote connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down prepare and so on Remote control allows easy connection between single instruments or s
164. se the test to fail Itis absolutely necessary that the pump is tight We recommend to run the Micro Mode Pressure Test to verify the tightness of the pump Running the Test 1 Prepare the restriction capillary G2226 67300 quartz capillary SST fitting front and back PEEK sleeve ferrule Slide the PEEK sleeve over the fused silica capillary Slide the SST fitting over the PEEK sleeve Slide the front and back ferrule over the PEEK sleeve uF W N Carefully press the capillary into an adjustment union and tight the fitting moderately with the 4 mm wrench 8710 1534 and the torque adapter G1315 45003 to fix the ferrule To reach right values for the flow sensor accuracy calibration the restriction capillary G2226 67300 must not be damaged or blocked 6 Select the ChemStation s Diagnosis screen from the nano pump tests selection box select Flow Sensor Accuracy Calibration Test 7 Start the test and follow the online instructions 1100 Series Nano Pump Service M anual 87 2 Troubleshooting and Test Functions Nano Pump Flow Sensor Accuracy Calibration fale Start Explain Close ee 8 The test results are evaluated automatically Test results In the ChemStation the measured values are evaluated automatically A table with the measured pressure drop over the inlet filter the measured flow and pressure offset and the correction factors for all steps
165. seals plungers and internal components for signs of wear contamination or damage Exchange components as required V Exchange the pump drive assembly 1100 Series Nano Pump Service Manual 59 2 60 Troubleshooting and Test Functions Index Adjustment Index Adjustment Pump channel A B Index Adjustment Pump channel B The encoder index position in the nano pump is out of adjustment During initialization the first plunger is moved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the time to reach the index position is too long the error message is generated Probable Causes e Irregular or sticking drive movement e Defective pump drive assembly Suggested Actions V Remove the pump head and examine the seals plungers and internal components for signs of wear contamination or damage Exchange components as required V Exchange the pump drive assembly 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Index Missing Index Missing Pump channel A B Index Missing Pump channel B The encoder index position in the nano pump was not found during initialization During initialization the first plunger is moved to the mechanical stop After reaching the mechanical stop the plunger reverses direction until the encoder index position is reached If the index position is not recognized within a d
166. sor checks the presence of the active inlet valve connector every 2 seconds If the connector is not detected by the processor the error message is generated Probable Causes e Disconnected or defective cable e Disconnected or defective connection cable front panel to CSPM board e Defective active inlet valve Suggested Actions V Ensure the pins of the active inlet valve connector are not damaged Ensure the connector is seated securely V Ensure the connection cable is seated correctly Exchange the cable if defective V Exchange the active inlet valve 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions Electro M agnetic Proportional Valve EM PV Missing EMPV Missing The EMPV in the micro pump is missing or defective Probable Causes e Disconnected or defective cable e Defective solenoid Suggested Actions V Ensure the connection cable is seated correctly V Exchange the solenoid of the EMPV 1100 Series Nano Pump Service Manual 2 53 2 Troubleshooting and Test Functions Flow sensor missing Probable Causes Flow sensor disconnected e Defective flow sensor Suggested Actions V Ensure the sensor is seated correctly V Exchange the flow sensor 54 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Unsupported Flow Sensor Probable Causes e A 20 ul min or a 100 ul min flow sensor is used with the nano pump Suggested Actions
167. ssing 51 fan failed 36 ignition without cover 37 index adjustment 60 index limit 59 index missing 61 initialization failed 63 inlet valve fuse 47 inlet valve missing 52 leak 31 leak sensor open 32 leak sensor short 33 missing pressure reading 44 motor drive power 50 pressure above upper limit 40 pressure below lower limit 41 pump head missing 58 restart without cover 38 selection valve failed 42 selection valve fuse 46 servo restart failed 53 shut down 28 stroke length 62 synchronization lost 29 temperature limit exceeded 49 temperature out of range 48 timeout 27 ESD Strap 106 exchanging active inlet valve 108 109 damper 141 271 Index fan 143 high pressure pump main board HPM board 134 interface board 129 internal parts 105 130 leak sensor 149 outlet ball valve 108 112 outlet ball valve sieve 108 112 pistons 108 125 power supply 147 pump drive 145 pump seals 108 122 purge valve 108 114 115 purge valve frit 108 114 115 solvent selection valve 118 Status light pipe 152 wash seals 108 126 external contacts 202 F fan 143 fan drive 208 fan failed 36 features GLP 259 instrument layout 204 safety and maintenance 259 firmw are description 213 main system 213 resident system 213 updates 214 irmware update with control module 251 first injection 17 flow connection diagram 16 flow range 258 flow sensor calibration 84 flow sensor pa
168. ssure limit Probable Causes e Upper pressure limit set too low e Blockage in the flowpath after the damper e Defective damper e Defective CSM board Suggested Actions V Ensure the upper pressure limit is set to a value suitable for the analysis V Check for blockage in the flowpath V Exchange the damper V Exchange the CSM board 40 1100 Series Nano Pump Service Manual Troubleshooting and Test Functions 2 Pressure Below Lower Limit The system pressure has fallen below the lower pressure limit Probable Causes Lower pressure limit set too high Air bubbles in the mobile phase Leak Defective damper Defective CSM board Suggested Actions V Ensure the lower pressure limit is set to a value suitable for the analysis V Ensure solvents are degassed Purge the nano pump V Ensure solvent inlet filters are not blocked V Inspect the pump head capillaries and fittings for signs of a leak V Purge the nano pump Run a pressure test to determine whether the seals or other pump components are defective V Exchange the damper V Exchange the CSM board 1100 Series Nano Pump Service Manual 41 2 42 Troubleshooting and Test Functions Pressure Signal Missing The pressure signal from the damper is missing The pressure signal from the damper must be within a specific voltage range If the pressure signal is missing the processor detects a voltage of approximately 120mV across the damper con
169. ssure test If the test fails again insert the blank nut at the outlet of the previous module in the stack and repeat the pressure test Exclude each module one by one to determine which module is leaky Potential Causes of Pressure Test Failure After isolating and fixing the cause of the leak repeat the pressure test to confirm the system is tight Potential Cause Pump Corrective Action Loose or leaky fitting Untight EM PV Damaged pump seals or plungers High flow sensor offset Tighten the fitting or exchange the capillary Run the EM PV test Run the leak test to confirm the leak Run the flow sensor accuracy calibration and correct the flow sensor offset Potential Cause Autosampler Corrective Action Loose or leaky fitting Needle seat Rotor seal injection valve Damaged metering seal or plunger Tighten or exchange the fitting or capillary Exchange the needle seat Exchange the rotor seal Exchange the metering seal Check the plunger for scratches Exchange the plunger if required 1100 Series Nano Pump Service Manual 67 2 68 Troubleshooting and Test Functions Normal M ode Pressure Test Pressure bar Step 1 Step 2 Description The pressure test is a quick built in test designed to demonstrate the pressure tightness of the system The test should be used when problems with leaks are suspected or after maintenance of flow path components e g pump se
170. st the restriction capillary the capillary going to the mixer the connector P16 P11 P20 at the main board see Figure 22 on page 135 7 Unclip the mixing chamber from its holder 8 Loosen the screws of the Z panel fold it forward and remove the damper 9 Re install the Z panel and the new damper Reconnect the capillaries and connectors P16 P11 P20 see Figure 22 on page 135 1100 Series Nano Pump Service Manual 141 3 142 Repairing the Pump 10 Re install the mixing chamber in its holder 11 Re install the flow sensor Reconnect the capillaries and the cable 12 Re install the EMPV Reconnect the capillaries the waste tube and the cable 13 Reconnect both active inlet valve cables 14 Replace the top foam section optional interface board front cover and top cover see Replacing the Top Cover and Foam on page 154 1100 Series Nano Pump Service Manual Repairing the Pump 3 Exchanging the Fan Frequency Fan not running Tools required Screwdriver Pozidriv 1 Wrench 1 4 inch Parts required Fan PN 3160 1017 1 Remove the pump from the stack remove the front cover top cover and top foam section see Removing the Top Cover and Foam on page 131 2 Remove the EMPV For this disconnect first the capillary going to the flow sensor the capillary coming from the filter the waste tube the connector 3 Remove the flow sensor For this disconnect first the capillary coming from the EMPV the
171. stic distributed over the two temperature sensors is evaluated From the temperature characteristic flow rate accuracy is determined The flow sensor measurement is calibrated for specific mobile phases which are user selectable 1100 Series Nano Pump Service Manual 201 5 Introduction to the Nano Pump Electrical Connections e The GPIB connector is used to connect the nano pump with a computer The address and control switch module next to the GPIB connector determines the GPIB address of your nano pump The switches are preset to a default address see Table 51 on page 219 and is recognized once after power on e The CAN bus is a serial bus with high speed data transfer The two connectors for the CAN bus are used for internal Agilent 1100 Series module data transfer and synchronization e One analog output provides a signal for integrators or data handling systems e The REMOTE connector may be used in combination with other analytical instruments from Agilent Technologies if you want to use features such as common shut down prepare and so on e The RS 232 connector may be used to control the nano pump from a computer via RS 232 connection using appropriate software This connector needs to be activated by the configuration switch module next to the GPIB connector The software needs the appropriate drivers to support this communication See your software documentation for further information e The power input socket accepts
172. t temperatures and relative humidity as described in Table 1 Do not store ship or use your nano pump under conditions where temperature fluctuations could cause condensation within the nano pump Condensation will damage the system electronics If your nano pump was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation Table1 Physical Specifications Type Specification Comments Weight 17 kg 39 Ibs Dimensions 180 x 345 x 435 mm height x weight x depth 7 x 13 5 x 17 inches Line voltage 100 120 or 220 240 VAC 10 Wide ranging capability 1100 Series Nano Pump Service Manual 3 1 Installing the Pump Table1 Physical Specifications continued Line frequency 50 or 60 Hz 5 Power consumption apparent power 220 VA Maximum Power consumption active power 75W Maximum Ambient operating temperature 4 55 C 41 131 F Ambient non operating temperature 40 70 C 4 158 F Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6500 ft Non operating altitude Up to 4600 m 14950 ft For storing the nano pump Safety standards IEC CSA UL Installation Category Il Pollution Degree 2 4 1100 Series Nano Pump Service Manual Installing the Pump 1 Unpacking the Nano Pump Damaged Packaging Upon receipt of your nano pump inspect the shipping containers for any signs of damage If the con
173. t the waste tube Beware of leaking solvents due to hydrostatic pressure Disconnect the EMPV connector P9 on the main board see Figure 22 on page 135 Using a hex key unscrew the complete EMPV and remove it Screw a new one in place Reconnect the capillaries going to the flow sensor coming from the filter Run the EMPV test see EMPV Test on page 84 1100 Series Nano Pump Service Manual 117 3 Repairing the Pump Exchanging the Solvent Selection Valve 118 Frequency If internally leaking crossfoot between the ports or if one of the channels is blocked Tools required Screwdriver Pozidriv 1 Parts required Solvent selection valve PN gives half of a complete solvent selection block G1312 60000 1 Disconnect the solvent tubes and the active inlet valve connection tubes from the solvent selection valves Place solvent tubes into the solvent cabinet to prevent leaks due to hydrostatic flow Solvent tubes Solvent selection valve Connecting tubes Figure 20 Exchanging the solvent selection valve 1100 Series Nano Pump Service Manual Repairing the Pump 3 2 Using a Pozidriv screwdriver 1 loosen the holding screws of the valves 3 Pull the valve module out of its connector 4 Hold the two plastic bodies of the valves and pull the two solvent selection valves apart 5 Exchange the defective solvent selection valve Press the exchanged valve mew half together with the properly wo
174. tainers or cushioning material are damaged save them until the contents have been checked for completeness and the nano pump has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carrier s inspection If there are signs of damage to the nano pump please do not attempt to install the nano pump Delivery Checklist Ensure all parts and materials have been delivered with the nano pump For this compare the shipment content with the checklist included in the instrument box Please report missing or damaged parts to your local Agilent Technologies sales and service office Accessory Kit Each shipment contents an accessory kit with the necessary tools to install the pump and to have an operating system Table 2 Nano Pump Accessory Kit Content G2226 68705 Description Part Number Insert tool 01018 23702 SST Solvent inlet filter x4 01018 60025 Waste tube 2 m 0890 1760 SST replacement frit 0 5 um 5022 2185 Wrench open end 7 16 1 2 inch x 2 8710 0806 1100 Series Nano Pump Service Manual 5 1 Installing the Pump Table 2 Nano Pump Accessory Kit Content G2226 68705 continued Description Part Number Wrench open end 1 4 5 16 inch x1 8710 0510 Wrench open end 14 mm x 1 8710 1924 Wrench open end 4 mn x 1 8710 1534 Hex key 2 5 mm 15 cm long straight handle x 1 8710 2412 Hex key 3 0 mm 12
175. te Figure 44 Firmware Update M echanism 214 1100 Series Nano Pump Service Manual Introduction to the Nano Pump 5 Optional Interface Boards The Agilent 1100 Series modules have one optional board slot that allows addition of an interface board to the modules Table 48 Optional Interface Boards Description Part Number BCD Board G1351 68701 Fuse 250 mA four are on the board 2110 0004 LAN Board see next page for details BCD Board The BCD board provides a BCD output for the bottle number of the Agilent 1100 Series well plate sampler and four external contacts The external contact closure contacts are relay contacts The maximum settings are 30 V AC DC 250 mA fused There is a general purpose cable available to connect the BCD output see BCD Cables on page 184 and the external outputs see External Contact Cable on page 189 to external devices 1100 Series Nano Pump Service Manual 215 5 Introduction to the Nano Pump Pos P2 Al Vial 601 ooo A Pos P2 B1 Vial 625 W Pos P2 P24 Vial 985 Pos P1 A1 Vial 201 O Pos P1 B1 Vial 213 W Pos P1 H12 Vial 297 O00 Plate 2
176. ted Actions V Replace the top foam V Exchange the CSM board V Ensure that the rear of the module is not exposed to strong sunlight 1100 Series Nano Pump Service Manual 37 2 38 Troubleshooting and Test Functions Restart Without Cover The nano pump was restarted with the top cover and foam open The sensor on the CSM board detects when the top foam is in place If the nano pump is restarted with the foam removed the nano pump switches off within 30 s and the error message is generated Probable Causes e The nano pump started with the top cover and foam removed e Rear of the module is exposed to strong direct sunlight Suggested Actions V Replace the top cover and foam V Ensure that the rear of the module is not exposed to strong sunlight 1100 Series Nano Pump Service M anual Troubleshooting and Test Functions 2 Zero Solvent Counter Pump firmware version A 02 32 and higher allow to set solvent bottle fillings at the ChemStation revision 5 xx and higher If the volume level in the bottle falls below the specified value the error message appears when the feature is configured accordingly Probable Causes e Volume in bottle below specified volume Incorrect setting of limit Suggested Actions V Refill bottles and reset solvent counters 1100 Series Nano Pump Service Manual 39 2 Troubleshooting and Test Functions Pressure Above Upper Limit The system pressure has exceeded the upper pre
177. thers which can contain peroxides for example THF dioxane di isopropylether such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Solutions of organic acids acetic acid formic acid and so on in organic solvents For example a 1 solution of acetic acid in methanol will attack steel Solutions containing strong complexing agents for example EDTA ethylene diamine tetra acetic acid Mixtures of carbon tetrachloride with 2 propanol or THF Avoid the use of alkaline solutions pH gt 8 5 which can attack the fuse silica from the capillaries 1100 Series Nano Pump Service M anual Safety Information A Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1100 series modules for download 1100 Series Nano Pump Service Manual 269 A Safety Information 270 1100 Series Nano Pump Service Manual Index A active inlet valve 108 109 active inlet valve drive 207 address switch 223 Agilent on internet 269 air flow 144 ambient non operating temperature 4 ambient operating temperature 4 analog output 202 258 analog signal output 219 APG remote connector 14 APG remote interface 220 ASIC application specific integrated circuit 207 assembling the ma
178. to the analysis view Select a module you want to move with the help of the selection keys Use function keys F7 F8 Remove Add to move the highlighted module Changes must be activated with Done F6 Here e g the autosampler and the thermostatted column compartment parameters are shown on the display as well The number of parameters for each module is reduced as additional modules are added to the display A maximum of 4 modules can be shown simultaneously If more modules are connected to the system you can choose up to 4 of them in Setup view i 25 23 C From the Analysis screen use the F7 key On Off to proceed to the turn on screen Press F8 On once to turn on the pump If more than one module is available select F8 Pump or turn on the entire system by selecting the System On button and pressing Enter 1100 Series Nano Pump Service Manual Settings Control Module Screens forthe Nano Pump 6 NEAM y SRDE cmu 0 008K Paad K Turn Pump On Off E to Standby mode only Pump Off use action keys E F3 to turn ontott individual modules System Off With the F1 key Settings you open a pull down menu where you can select the capillary pump module with the help of the selection or alphanumeric keys Analysis Line E Loc DEI Wia HOD Idie Ready Thu 13 11 B 1 5 1x ACTUAL 376 2 00 a Ready ZE cous 10 Column Flow wh Channel aa Onort
179. ument are furnished under a license and may be used or copied only in accor dance with the terms of such license Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating pro cedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated conditions are fully understood and met A WARNING notice denotes a haz ard It calls attention to an operat ing procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met Warning Symbols Used on the Instrument The apparatus is marked with this symbol when the user should refer to the instruction manual in order to prevent risk of harm to the operator and to protect the apparatus against damage 1100 Series Nano Pump Service M anual In This Guide Installing the Pump How to install the nano pump Troubleshooting and Test Functions The nano pump s built in troubleshooting and test functions Repairing the Pump Instructions on simple routine repair procedures as well as more extensive repairs requiring exchange of internal parts Parts and Materials Detailed illustrations and lists for identification of parts and materials Intro
180. uration Rear View 1100 Series Nano Pump Service Manual 9 1 Installing the Pump Detector ossos o Control M odule coe a N eS 3 4 z arg i wo 2 oo Da iS f T MH A Column 7 Compartment F TS Oy J Ce Solvent Cabinet a SS Autosampler Degasser E C i Dy na i Ea Pump ALS Thermostat ump l ow Co f Gy ES Figure3 Recommended stack configuration front view 10 1100 Series Nano Pump Service M anual Installing the Pump 1 Analog signal GPIB or LAN to to recorder gt LC ChemStation
181. ven if the power switch on the front panel is turned off Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified Power Cords Different power cords are offered as options with the nano pump The female end of each of the power cords is identical It plugs into the power input socket at the rear of the nano pump The male end of each of the power cords is different and designed to match the wall socket of a particular country or region Never operate your instrumentation from a power outlet that has no ground connection Never use a power cord other than the power cord designed for your region 1100 Series Nano Pump Service Manual Installing the Pump 1 Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Bench Space The nano pump dimensions and weight see Table 1 allow to place the nano pump on almost any laboratory bench It needs an additional 2 5 cm 1 0 inches of space on either side and approximately 8 cm 3 1 inches in the rear for the circulation of air and electric connections If the bench should carry a complete Agilent 1100 Series system make sure that the bench is designed to carry the weight of all the modules The pump should be operated in a horizontal position Environment Your nano pump will work within specifications at ambien
182. wer switch at the front of the pump is off 12 1100 Series Nano Pump Service Manual Installing the Nano Pump Installing the Pump 1 Place the pump on top of the micro sampler Make sure that the two modules are interlocked correctly 1 2 Connect the power cable to the power socket at the rear of the pump Do not connect the power cable to power until you have finished the hardware installation of all modules in the stack Configuration switch Slot for interface board H RS232 SS Remote E Analog output OED CED Ojo CAN Bus Une Q oe es Bo A viem C 2 E gt C ae gt C ai CERE ajsja 7 amp e g 2 OD OD A ee m eT Es gt 5s SS eer tee oe eT Er c e l MTT woe n 2 E M ere HATTA TTA NT i a gt a pa ak gt Power Plug Security Lever Figure 5 Rear of Nano Pump 3 Connect the free end of the Can bus cable from the micro sampler to one of the Can bus connectors at the rear of the pump 1100 Series Nano Pump Service Manual 13 1 Installing the Pump In an Agilent 1100 Series system the individual modules are connected through CAN cables The Agilent 1100 Series vacuum
183. ystems to ensure coordinated analysis with simple coupling requirements The subminiature D connector is used The module provides one remote connector which is inputs outputs wired or technique To provide maximum safety within a distributed analysis system one line is dedicated to SHUT DOWN the system s critical parts in case any module detects a serious problem To detect whether all participating modules are switched on or properly powered one line is defined to summarize the POWER ON state of all connected modules Control of analysis is maintained by signal readiness READY for next analysis followed by START of run and optional STOP of run triggered on the respective lines In addition PREPARE and START REQUEST may be issued The signal level is defined as e standard TTL levels 0 V is logic true 5 V is false e fan out is 10 e input load is 2 2 kOhm against 5 V and e outputs are open collector type inputs outputs wired or technique Table 52 Remote Signal Distribution Pin Signal Description 1 DGND Digital ground 2 PREPARE L Request to prepare for analysis for example calibration detector lamp on Receiver is any module performing pre analysis activities 3 START L Request to start run timetable Receiver is any module performing run time controlled activities 4 SHUT DOWN L System has serious problem for example leak stops pump Receiver is any module capable to reduce safety risk

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