Eppendorf Centrifuge 5424 - biovendis
Contents
1. Place the centrifuge on a solid flat non resonant lab bench Check beforehand whether the lab bench is specified for the weight of the centrifuge The surrounding area must be well ventilated and protected against direct sunlight To ensure that ventilation of the device is not impaired a minimum fundamental clearance of at least 30 cm to the side and at least 15 cm to the back wall must be maintained During centrifugation according to the recommendations set out in EN 61010 2 020 a safety clearance of 30 cm must be maintained around the centrifuge within which there are no objects which may be destroyed and so cause further damage Please ascertain that the power supply and the power frequency are compatible with the information given on the device ID plate 1 Introduction Now connect the centrifuge to the power supply and switch it on at the main power Switch see inside cover page The centrifuge is now ready for operation and the display is active Before starting for the first time check whether the rotor and the rotor lid are tightened in accordance with specification To tighten the rotor place the rotor key supplied on the rotor nut and turn it clockwise until the rotor nut is firmly tight ened up The rotor lid is then tightened up uonoanpoaju 29 Safety precautions and applicational limitations 30 2 Safety precautions and applicational limitations For your personal safety please be sure to comp2. Report prepared for Eppendorf AG Hamburg Germany Issue Date 10 March 2008 Test Summary Rotor FA 45 24 11 5424 700 101 00 50 x autoclaved at 121 C for 20 minutes was containment tested in the Eppendorf 5424 centrifuge using Annex AA of IEC 1010 2 20 The rotor was shown to contain a spill within the rotor Report Written By Report Authorised By Menu structure Men struktur M1 M2 BACK SOFT BACK see Section 3 11 ON SOFT siehe Kapitel 3 11 OFF ALARM BACK see Section 3 14 ON d siehe Kapitel 3 14 OFF x ATSET BACK see Section 3 12 ON v siehe Kapitel 3 12 OFF 2 SHORT BACK see Section 3 8 MAX siehe Kapitel 3 8 SHORT SLEEP BACK see Section 3 16 ON siehe Kapitel 3 16 OFF LOCK BACK see Section 3 13 ON o siehe Kapitel 3 13 OFF o VOL BACK see Section 3 15 VOL1 siehe Kapitel 3 15 VOL2 VOL3 VOL4 VOL5 132 Eppendorf Offices AUSTRALIA NEW ZEALAND Eppendorf South Pacific Pty Ltd Tel 61 2 98 89 50 00 Fax 61 2 98 89 51 11 E Mail Info eppendorf com au Internet www eppendorf com au AUSTRIA Eppendorf Austria Tel 43 1 2901756 0 Fax 43 1 2901756 20 E Mail office eppendorf at Internet www eppendorf at BRAZIL Eppendorf do Brasil Ltda Tel 55 11 3095 9344 Fax 55 11 3095 9340 E Mail eppendorf eppendorf com br Internet www eppendorf com br CANADA Eppendorf Canada Ltd Tel 1 905 826 5525 Fax 1 905 826 5424 E Mai
3. Centrifuge 5424 eppendorf Table of contents Centrifuge 5424 Inhaltsverzeichnis 1 0 5 Instruction Manual accio dd qd i d EX RII Apr SRL CI eI PEDE UT 27 Notice abr g e EE 49 lstruzioni in breve 20 58 Instr cciones breves 5 67 Beknoptehandleiditig Sie ain iia ete ec 11111 9 9 76 Kort vejledning uer ata th Yu el dh e q end 85 Kort handlednilag s Ki Mea 1 1 1 Een 3 1 90 94 PIKAOHJE ar d 3 isn ee 103 Manual abreviado 3 31 111 EUVOTTIKEG OONVIEG ae ern nee tem 1 459 120 No part of this publication may be reproduced without the prior permission of the copyright owner Copyright 2005 Eppendorf AG Hamburg Figure 1 Abbildung 1 Centrifuge 5424 Figure 1 Abbildung 1 2 4 3 open K short menu enter start stop Arrow button speed rpm rcf time N So H menu enter menu enter button menu enter Taste Arrow button Arrow button Pfeiltaste rpm rcf rpm rcf button rpm rcf Taste time Time arrow buttons Zeitwahl Pfeiltasten speed Speed arrow buttons Geschwindigkeits Pfeiltasten open Lid release button Deckelentriegelungs Taste short Short run button Kurzzeitlauf Taste start stop Start stop button Start Stop Taste 1 Power plug Netzstecker 2 Power switch Netzschalter 3 Rotor nut Rotormutter 4 Rotor Rotor E Emergency lid release Notentriegelung Figure 2 Abbildung 2 Centrifuge 5424 Figur
4. During braking the timer flashes and shows the spin time which has elapsed When the rotor has come to a standstill a signal tone is heard and the centrifuge lid opens automatically The display then shows the symbol 3 Operation 3 10 rcf display and calculation rpm rcf Pressing the button toggles the display from rpm 1 min to rcf and vice versa Please note that the g force rcf shown when toggling the display is standardized to rotor FA 45 24 11 FA 45 24 11 Special without adapter At the maximum speed in each case you can achieve the following maximum g force rcf in the various rotors with the different adapters Rotor adaptor s FA 45 24 11 without adapter 8 4 20 238 21 130 0 2 ml 6 3 15 179 15 848 0 4ml 8 4 20 238 21 130 0 5 ml 73 17 588 18 363 0 6 ml 84 20 238 21 130 F 45 18 11 Kit without adapter 72 17 347 18 111 0 2 ml 5 1 12 288 12 829 0 4ml 72 17 347 18 111 0 5 ml 6 1 14 697 15 345 0 6 ml 72 17 347 18 111 F 45 32 5 PCR 7 4 17 829 18 615 To calculate the g force rcf for a specific adapter you can apply the following formula accord ing to DIN 58 970 rcf 1 118 10 n tax n speed in rpm fmax Max centrifugal radius in cm Example in rotor FA 45 24 11 the 0 5 ml adapter has a maximum radius of 7 3 cm At 7 000 rpm a maximum g force rcf of 4 000 x g is achieved 37 0 40 Operation 38 3 Operation 3
5. lke amp Mayr GmbH Norderstedt Germany RBS neutral Registered trade mark of Carl Roth GmbH Co KG Karlsruhe Germany Teepol 610S Registered trade mark of Sigma Aldrich Corp St Louis USA Le um M7 scht o ae un ka Le um e E L Vx ur c C ce C e C ce L ce C ce Te CE ce C GK e CE ES ce E ce Se ce weg CE C eS ice ec cC C t t ce Ko UT rr p n c ce ei ce vi ce Ce c Te Go S ce e ce C e E ce Ce Go ce C C C C EL et Ee ce C tE Se C ve C e C e C e C ce AAA x c ce m lt et 6 al ce ART EG Konformit tserkl rung CEE E E ce E ce EE et c ce EC Conformity Declaration a ce C di ri qe ce Das bezeichnete Produkt entspricht den einschl gigengrundlegenden Anforderungen der c e LE s ce ce C aufgef hrtenEG Richtlinien und Normen Bei einer nicht mit uns abgestimmten nderung des t C ce c t ce Produktes oder einer nicht bestimmungsgem Sen Anwendung verliert diese Erklrungihre G Itigkeit c ct i y L e c The product named below fulfills the relevant fundamental requirements of ce e ce c C ce C the EC directives and standards listed In the case of unauthorized modifications to the product ce e CE ce ce ce or an unintended use this declaration becomes invalid ce p c C u C ce pr 3 oi ce 6 CE An C Te C D ce ce c c Gei ce S Produktbezeichnung Product name AN c E C qe c i e Sch Centr
6. symbol During a short run all other buttons and knobs are blocked Specified speed is set via the menu menu enter activates the menu select the item SHORT and confirm with menu enter in the second menu level select the item MAX in order to centrifuge at maximum speed g force To perform short run centrifugation at the currently set speed g force select the item SET with the arrow button menu enter confirms the set speed and you automatically return to the item BACK in the first menu level Press the key again to exit the menu If the specified speed is not to be changed after all it is possible to exit the second menu level by selecting the BACK menu item and then confirming with the menu enter button 3 9 Continuous operation Switch on the centrifuge with the power switch if necessary and open the lid using the open button The specified values of the last run are displayed Load the rotor symmetrically fit the rotor lid and close the centrifuge lid time the continuous function is set using the time selection knob arrow buttons to above 09 59 h or below 30 sec The timer shows oo to indicate continuous run ning Time is counted upwards in 30 second increments speed adjusts the speed in increments of 50 rpm or the g force rcf in increments of 50 xg start stop starts centrifugation The symbol M flashes while the rotor is running start stop if the button is pressed again centrifugation will stop
7. 120 V 100 V Set of fuses Rotor code Order no International 5424 706 002 5424 707 009 5424 704 000 5424 708 005 5425 715 005 5425 717 008 5425 716 001 5416 301 001 5703 350 102 5424 852 122 5424 852 130 Order no North America 022653083 022653105 022653121 022653148 022636260 022636243 022636227 022634305 022639609 950004266 950004240 uoneuuojutr BULIDPAO All Eppendorf rotors are designated according to a simple logical system which describes the technical specifications as a uniform series of numbers and letters e g Fixed angle Angle of Tube tube adapter rotor adapter bore Adapter Swing bucket rotor bore I I I I I F A 45 30 11 A 4 81 I I I Aerosol tight Max no tubes Max no tubes version adapters adapters Ordering information 48 7 Ordering information Important Use only the original accessories we recommend The functioning and safety of centrifuges may be impaired if you use spares or disposables other than those we recommend Any warranty and liability for losses thus caused shall be excluded Bacillol AF Registered trade mark of Bode Chemie GmbH amp Co Hamburg Germany Extran neutral Registered trade mark of Merck KgaA Darmstadt Germany Meliseptol Registered trade mark of B Braun Melsungen AG Melsungen Germany Microtainer Registered trade mark of Becton Dickinson Franklin Lakes USA Perform Registered trade mark of Sch
8. accessories in addition to loss of the sample Tube lids must be sealed down tight before centrifuging The lids of tubes which are not sealed may rip off during centrifugation and damage the rotor lid and the centrifuge When using organic solvents e g phenol chloroform the durability of plastic tubes may be impaired with the result that tubes may break during centrifugation When closing the centrifuge lid do not place your fingers between the lid and the centri fuge otherwise they may be trapped The transparent rotor covers made from polypropylene have a maximum useful life of 3 years The date of manufacture is impressed on them in the form of a watch D Rotors and rotor covers which have been damaged chemically or mechanically or which have passed their maximum useful life must no longer be used Declaration concerning the ATEX directive 94 9 EC The present design and ambient conditions inside Eppendorf centrifuges mean that they are not suitable for use in a potentially explosive atmosphere The centrifuges must therefore only be used in a safe environment such as the open environment of a ventilated laboratory or a fully extracted fume hood The use of substances which may contribute to a potentially explosive atmosphere is not permitted The final decision on the risks in connection with the use of such substances is the responsibility of the user of the centrifuge 31 N 0 jeu
9. 11 Setting startup and braking ramp When setting the startup and braking ramp you can select between two levels Selection is made using the menu menu enter activates the menu select the item SOFT and confirm with menu enter in the second menu level select the item ON for the rotor to start up and brake more slowly and the item OFF for it to start up and brake more quickly menu enter confirms the amended startup and braking ramp and you automatically return to the item BACK in the first menu level Press the key again to exit the menu If the startup and braking ramp is not to be changed after all it is possible to exit the second menu level by selecting the BACK menu item and then confirming with the menu enter button If the slower startup and braking ramp is switched on the symbol appears in the display 3 12 Run start For centrifugation time can be counted either immediately from the start or not until attainment of the preset speed or g force rcf This is specified via the menu menu enter activates the menu select the item ATSET and confirm with the second menu level select the item ON for time to the counted only once the set speed g force rcf has been reached Select the item OFF if time is to be counted from the start of centrifugation menu enter confirms the selected run start and you automatically return to the item BACK in the first men
10. 11 Special with rotor lid aluminum aerosol tight coated angle 45 24 places max diameter 11 mm designed for 1 5 2 0 ml micro test tubes Spare lid aluminum for rotor FA 45 24 11 Special aerosol tight coated Orderno International 5424 000 614 5424 000 215 5424 000 410 5424 000 010 5424 000 622 5424 000 223 5424 000 428 5424 000 029 5424 702 007 5424 703 003 5424 700 004 5424 701 000 Order no North America 022620436 022620452 022620428 022620461 022620487 022620498 022620401 022620444 022653008 022653024 022653041 022653067 7 Ordering information Fixed angle rotor F 45 18 11 Kit with rotor lid aluminum angle 45 18 places max diameter 11 mm designed for 1 5 2 0 ml micro test tubes Spare lid polypropylene for rotor F 45 18 11 Kit Fixed angle rotor F 45 32 5 PCR with rotor lid aluminum angle 45 32 places max diameter 5 mm designed for 0 2 ml PCR tubes Spare lid aluminum for rotor F 45 32 5 PCR Accessories Adapter for 0 2 ml PCR tubes for FA 45 24 11 FA 45 24 11 Special and F 45 24 11 Kit per 6 pcs Adapter for 0 4 ml micro test tubes for FA 45 18 11 FA 45 18 11 Special and F 45 24 11 Kit per 6 pcs Adapter for 0 5 ml micro test tubes and 0 6 ml Microtainers for FA 45 18 11 FA 45 18 11 Special and F 45 24 11 Kit per 6 pcs Rotor key Captain Eppi rotor key holder 1 pc 2 x 3 15 AT 230 V 2 x 6 3 AT
11. 2 2 No 1010 2 0204 97 Part 2 Part Requirements for Laboratory Centrifuges Additional Information ELECTRICAL RATING Voltage 120 V ac Frequency 50 60 Hz Current 3 8 A Power 220 W Only those products bearing the UL Listing Mark for the US and Canada should be considered as being covered by UL s Listing and Follow Up Service meeting the appropriate requirements for US and Canada Ihe UL Listing Mark for the US and Canada generally includ n circle symbol with C and US identifiers Ou i word LISTED a cont umber ma alphanumeric assigned by UL and the product category name product identifier indicated in the appropriate UL Directory Look for the UL Listing Mark on the product gt Cyn uy Le Issued by Walter Hofmair Reviewed by x Manfred Waller Walter Hofmair Senior Project Engineer Manfred M ller Senior Project Engineer UL Intemational Germany GmbH UL International Germany GmbH Any information and documentation provided to you involving UL Mark services are provided on behalf of Underwriters Laboratories Ine For questions in Germany you may call 49 0 61 02 369 0 Centre of Emergency Preparedness and Response Health Protection Agency Porton Down Health t Salisbury Wiltshire SP4 0JG T gt United Kingdom Certificate of Containment Testing Containment Testing of Rotor FA 45 24 11 5424 700 101 00 50 x autoclaved at 121 C for 20 minutes in Eppendorf Centrifuge 5424 Report No 73 08 A
12. Nordic Aps Tel 45 70 22 29 70 Ss Fax 45 45 76 73 70 E Mail nordic eppendorf dk Internet Internet www eppendorf dk www eppendorf com worldwide SOUTH amp SOUTHEAST ASIA Eppendorf Asia Pacific Sdn Bhd Tel 60 3 8023 2769 Fax 60 3 8023 3720 E Mail asiapacifichq eppendorf com my Internet www eppendorf com my eppendorf In touch with life Your local distributor www eppendorf com worldwide Eppendorf AG 22331 Hamburg Germany Tel 49 40 538 01 0 Fax 49 40 538 01 556 E Mail eppendorf eppendorf com Eppendorf North America Inc One Cantiague Road PO Box 1019 Westbury N Y 11590 0207 USA Tel 1 516 334 7500 Toll free phone 1 800 645 3050 Fax 1 516 334 7506 E Mail info eppendorf com Application Support Europe International Tel 49 1803 666 789 E Mail support eppendorf com North America Tel 1 800 645 3050 ext 2258 E Mail support_na eppendorf com Asia Pacific Tel 60 3 8023 6869 E Mail support_asiapacific eppendorf com eppendorf is a registered trademark B 5424 900 011 04 0408 Printed in Germany
13. al hazardous to health or aggressive material contaminate the device the owner is responsible for appropriate cleaning and decontamination Before cleaning unplug the power plug with the lid open unscrew the rotor using the rotor key supplied and clean it separately Use only neutral agents for cleaning e g Extran neutral RBS neutral Teepol 610 S Bacillol AF Meliseptol und Perform are recommended for cleaning and disinfecting the outer surface of the centrifuge and the rotor chamber Do not allow any liquid to get into the gap at the motor shaft outlet For this reason the rotor chamber should be cleaned only with a damp cloth The outer surface of the centrifuge and the rotor chamber have been tested for resistance to the cleaning agents and disinfectants mentioned However this does not guarantee that the device is disinfected following application of one of the methods mentioned You should also consult your laboratory safety officer with regard to a suitable method of cleaning and disinfecting Before any cleaning or disinfecting method other than that recommended by the manufacturer is used please check with Eppendorf that the intended method will not damage the device or its accessories In order to ensure long term reliable work with your centrifuge please note that aggressive chemicals may damage the rotor and the chamber Check your device once a month for corrosion and damage The rubber seals in the rotor chamber sh
14. d will not damage the rotors or their accessories In order to ensure long term reliable work with your centrifuge please note that aggressive chemicals may dam age the rotor 4 Maintenance and cleaning All the rotors rotor lids and adapters can be autoclaved 121 C 20 min The lids of aerosol tight rotors FA 45 24 11 and FA 45 24 11 Special should be replaced after no more than 50 autoclaving operations On the aerosol tight rotors the rotor lids should be replaced if the sealing rings on the lid thread and in the lid groove become worn Regular care of the sealing rings is required to protect the rotors Check that the seals are undamaged before use Aerosol tight rotors may not be stored with lids done up tightly 4 3 Glass breakage When centrifuging glass tubes be aware that as speed rcf increases so does the risk of glass breaking Please observe manufacturers information about maximum loading of centrifuge tubes In case of glass breakage carefully remove all splinters and all ground glass from the rotor the adapters and the rotor chamber You may need to replace adapters in order to prevent further damage Otherwise fine glass splinters will scratch the surface of the rotors reducing their resistance to chemicals Air vortices will result in very fine black abraded metal in the rotor chamber in addi tion to damaging the rotor chamber rotor and adapters this material will also cause samples to become contaminat
15. display and calculatiori 212r ee ea 37 Setting startup and braking ramp en Permanent setting of parameters Activating and deactivating the loudspeaker sh uuau aman seen neser evente eee 39 Vol m COMO iii 80 40 1 erret 40 Opening the centrifuge in case of power failure 41 2 1 1 41 Maintenance and 42 zu A DR IE 7 1 0 Glass breakage T RetutningideviGes 3 1150 43 E 44 Technical Qalancsncicsincicsm sscis nd cc ia dhie nauuna r dassie sesi b n sd s veb sone r 45 Ordering information kan ann anon en oren se tenor e none enes te rra 46 27 0 JO 1 Introduction 28 1 Introduction The Centrifuge 5424 is a non refrigerated bench top centrifuge It is intended for sample preparation within the sphere of clinical diagnostics and in the routine training and research laboratory in hospitals science and industry The devices may only be operated by trained specialist staff The vari
16. e 2 Abbildung 2 3 open 8 NO short ee Or rpm rcf y i Le O c EN time X en menu enter menu enter button menu enter Taste Arrow button Arrow button Pfeiltaste rpm rcf rpm rcf button rpm rcf Taste time Time knob Zeitwahl Drehknopf speed Speed selector knob Geschwindigkeits Drehknopf open Lid release button Deckelentriegelungs Taste short Short run button Kurzzeitlauf Taste start stop Start stop button Start Stop Taste 1 Power plug Netzstecker 2 Power switch Netzschalter 3 Rotor nut Rotormutter 4 Rotor Rotor E Emergency lid release Notentriegelung Table of contents N No st 3 2 3 3 3 4 3 5 3 6 3 7 3 8 3 9 3 10 3 11 3 12 3 13 3 14 3 15 3 16 3 17 3 18 4 1 4 2 4 3 4 4 Introductton m 28 Delivery package H 28 ioeie iare aae CN e e 1 dt 28 Safety precautions and applicational limitations eere 30 E a ci dd 33 Functional and operator control elemente nn 33 eire 2 2 33 3 3 7 6 Fitting and removing the rotors Br Loading the rotors si aisen een BM TT Centrifugation with timer setting core never ece ce serre reete nennen ennt one center esesese Adjusting centrifuging parameters during centrifugation kaun uaaaaaaaaaanaaaane seen veseve eee 35 Short spin centrifugation 2 RS A is i EEA RES ES SAET KA SERE SA SEP n ret
17. e lid again and start Lid cannot be unlocked Switch device off and back on press the open button if error recurs switch off device activate emergency lid release if error recurs gt Service Lid cannot be unlocked Wait for centrifuge to come to a during a run standstill repeat run if error recurs gt Service INT Power interruption Check power plug during a run NO RPM Error in speed Leave device switched on until the measuring system error message disappears 10 s or 6 min repeat run if error recurs gt Service Err 6 Drive error Repeat run if error recurs gt Service Err 7 Overspeed or major Check rotor properly tightened control deviation repeat run Err 8 Rotor loose drive error Tighten up rotor repeat run if error recurs gt Service Err 11 Power interruption Check power plug repeat run during arun Err 9 10 12 17 Electronics error Repeat run if error recurs gt Service 6 Technical data Mains power connection Power output Max speed Max relative centrifugal force rcf Max load Max kinetic energy Permissible density of material to be centrifuged Ambient temperature Relative humidity Setup height Dimensions Weight excluding rotor Startup time 230 V Deceleration time 230 V Startup time 120 V Deceleration time 120 V Startup time 100 V Deceleration time 100 V Fuses Noise level Overvoltage category Degree of contamination Technical specificatio
18. ed Check the rotor bores regularly for residues or damage 4 4 Returning devices When returning centrifuges ensure that these devices are fully decontaminated and do not present any kind of health risk to our service staff For further information and a blank of the decontamination confirmation please visit www eppendorf com Do also consult your laboratory safety officer about a suitable deconta mination method Please fill in the decontamination confirmation and enclose it with the device if it is to be returned to Eppendorf 43 Bulueap pue Troubleshooting guide 44 5 Troubleshooting guide If the suggested rectification measures repeatedly fail contact Eppendorf Error Display Cause Remedy No display No power Power failure Check power connection Check power fuses of device and laboratory Lid cannot be opened Power failure Rotor still running See above activate emergency lid release Wait for rotor to stop Centrifuge will not start Lid not closed Press lid shut Centrifuge shakes when starting up Rotor unevenly loaded Stop centrifugation and load evenly Centrifuge brakes during a Button was released Do not release button during a short short run although the briefly more than 2x run short button is still drive protection depressed function LID ERROR Lid cannot be locked Clos
19. ening the centrifuge in case of power failure N If the lid release does not function following a power failure the emergency lid release can be operated by hand To do so first unplug the power plug Before activating the emergency release also ensure that the rotor has come to a standstill Check by looking through the window in the centrifuge lid It can take up to 6 minutes for the rotor to come to a standstill The emergency release is located on the right hand side of the centrifuge see E in Figure 1 2 first inside cover page After a visual check that the rotor has come to a standstill first remove the plastic cap The rotor key supplied then has to be inserted in the nut underneath the cap and turned anticlockwise This releases the lid and allows it to be opened The rotor key must then be removed immediately and the plastic cap put back on 3 18 Device fuses The fuse box is located under the power plug To replace the fuses unplug the power plug and pull the fuse box out towards the rear The two fuses can then be replaced see Ordering information 41 0 3 40 Maintenance and cleaning 42 4 Maintenance and cleaning 41 Device The outer surfaces of the centrifuge and the rotor chamber should be cleaned regularly with a neutral agent e g Extran neutral RBS neutral or Teepol 610 S This is for hygiene pur poses and to prevent adhering impurities causing corrosion If materi
20. ifuge 5424 ee c C 4 Cc e Bee 2 046 C o Drs ce Ka einschlie lich cluding accessories i ei Y 1 etc e c x ce yr t i kri e Te c c Laboratory Centrifuge 6 pr C i oi ct e e Se C Ce f LS t C ce Ee ce Te e C ct A er C inien Normen Relevant EC directives ste p ce x eS ce So e 0 1 EN 61010 2 20 a C e Eu Si SoS 200410856 ag C C cc 98 37 EG EN 292 2 EN Ct CS e e a ce Te D ce ce erre KE S c E E CE Projektmanagement Project Management C a 6 0015 033 509 02 ci V LE v e C ek C Ce C T t VE PELE C KE Se Certificate of Compliance Certificate Number 150705 E215059 Page 1 of 1 Report Reference E215059 June 21st 2005 Issue Date 2005 July 15 Underwriters Laboratories Inc Issued to E P P EN DO RF AG BARKHAUSENWEG I 22339 HAMBURG GERMANY This is to certify that Centrifuge representative samples of Model 5424 Have been investigated by Underwriters Laboratories Inc in accordance with the Standard s indicated on this Certificate jw Cofoty UL 61010A 1 Electrical Equipment for Laboratory Use Part 1 Standard s for Safety _ 1 610104 2 020 Part 2 Particular Requirements for Laboratory Centrifuges CSA C22 2 No 1010 1 92 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements CSA C22 2 No 1010 2 020 92 CSA C2
21. l canada eppendorf com Internet www eppendorfna com CHINA Eppendorf China Ltd Tel 86 21 68760880 Fax 86 21 50815371 E Mail market info eppendorf cn Internet www eppendorf cn FRANCE EPPENDORF FRANCE S A R L Tel 33 1 30 15 67 40 Fax 33 1 30 15 67 45 E Mail eppendorf eppendorf fr Internet www eppendort fr GERMANY SPAIN Eppendorf Vertrieb Deutschland GmbH Tel 49 2232 418 0 Fax 49 2232 418 155 E Mail vertrieb eppendorf de Internet www eppendorf de Eppendorf Iberica S L U Tel 34 91 651 76 94 Fax 34 91 651 81 44 E Mail iberica eppendorf es Internet www eppendorf es SWITZERLAND INDIA Vaudaux Eppendorf AG Tel 41 61 482 1414 Fax 41 61 482 1419 E Mail vaudaux vaudaux ch Internet www eppendorf ch Eppendorf India Limited Tel 91 44 42 11 13 14 Fax 91 44 42 18 74 05 E Mail info eppendorf co in Internet www eppendorf co in UNITED KINGDOM ITALY Eppendorf UK Limited Tel 44 1223 200 440 Fax 44 1223 200 441 E Mail sales eppendorf co uk Internet www eppendorf co uk Eppendorf s r l Tel 390 2 55 404 1 Fax 390 2 58 013 438 E Mail eppendorf eppendorrf it Internet www eppendorf it USA JAPAN Eppendorf North America Tel 1 516 334 7500 Fax 1 516 334 7506 E Mail info eppendorf com Internet www eppendorfna com Eppendorf Co Ltd Tel 81 3 5825 2363 Fax 81 3 5825 2365 E Mail info eppendorf jp Internet www eppendorf jp NORDIC Eppendorf
22. lar attention should be paid to the fact that tube lids are closed in accordance vvith specification before centrifugation Aerosol tight centrifugation can only be carried out with the rotor lid in place Spin Columns must always be centrifuged with the rotor lid The rotor lid is not required during standard operation With the rotor F 45 18 11 Kit up to 18 Spin Columns can be centrifuged at a maximum of 14 680 rpm 120 V 15 000 rpm or 17 347 x g 120 V 18 111 x g This rotor is characterized by a particularly high edge so that all standard Spin Columns fit under the rotor lid But it is also possible to load the rotor with 1 5 2 0 ml micro test tubes and using the appropriate adapters with 0 2 ml PCR tubes 0 4 ml micro test tubes 0 5 ml micro test tubes and 0 6 ml Microtain ers The maximum load adapter tube and contents per bore for this rotor is 3 75 g Rotor F 45 18 11 Kit may only be centrifuged with the rotor lid tightened in accordance with specification 3 Operation With rotor F 45 32 5 PCR up to 32 PCR tubes 0 2 ml or four 5x or 8x rows of 0 2 ml PCR tubes can be centrifuged at maximum 14 680 rpm 120 V 15 000 rpm or 17 829 x g 120 V 18 615 x g Maximum load tubes and contents per 8x row with this rotor is 3 5 g Rotor F 45 32 5 PCR may only be centrifuged with the rotor lid tightened in accordance with specification When loading rotors make sure that the micro test tubes are inserted in the rotor bores oppo
23. ly with the following regulations unconditionally The centrifuge 5424 must only be used for the specified applications see Section 1 Introduction It must not be operated in explosive atmospheres Explosive radioactive or highly reactive substances must not be centrifuged When being moved from the cool room to a normal lab environment the centrifuge must either run for half an hour in the cool room first to warm up or it must warm up for at least 3 hours in the lab before being connected to the power supply system in order to prevent damage from condensation The centrifuge must not be moved or knocked while in operation Centrifuges which have not been properly installed or repaired may not be operated Repairs may only be carried out by Service personnel authorized by Eppendorf Use only original Eppendorf spare parts and rotors When handling toxic liquids or pathogenic microorganisms of risk group II see World Health Organization Laboratory Biosafety Manual comply with the relevant national regulations Bioseals are a component of biosafety systems which are not capable in isolation of ensuring that people and the environment are protected when pathogenic microorganisms are being handled When working with pathogenic microorganisms of a higher risk group more than one aerosol tight bioseal must be provided for If the named liquids are spilled in the rotor or rotor chamber the centrifuge must be thoroughly and professionall
24. ns subject to change 230 V 50 60 Hz 120 V 50 60 Hz 100 V 50 60 Hz 250 W 100 to 14 680 rpm 230 V 100 V 100 to 15 000 rpm 120 V 20 238 x g 230 V 100 V 21 130 x g 120 V 24 x 2 0 ml micro test tubes 7500 Nm 1 2 g ml 2 40 C 10 75 96 max 2000 m above NSL Width 236 mm Depth 320 mm Height 227 mm 13 4 kg 15s 16s 15s 18s 20s 16s 3 15 AT 230 V 6 3 AT 120 V 100 V 56 dB A T 2 45 0 1 Ordering information 46 7 Ordering information Centrifuge 5424 with knobs without rotor 230 V 50 60 Hz Centrifuge 5424 with keypad without rotor 230 V 50 60 Hz Centrifuge 5424 with knobs with rotor FA 45 24 11 incl rotor lid 230 V 50 60 Hz Centrifuge 5424 with keypad with rotor FA 45 24 11 incl rotor lid 230 V 50 60 Hz Centrifuge 5424 with knobs without rotor 120 V 50 60 Hz Centrifuge 5424 with keypad without rotor 120 V 50 60 Hz Centrifuge 5424 with knobs with rotor FA 45 24 11 incl rotor lid 120 V 50 60 Hz Centrifuge 5424 with keypad with rotor FA 45 24 11 incl rotor lid 120 V 50 60 Hz Fixed angle rotors and rotor lids Fixed angle rotor FA 45 24 11 with rotor lid aluminum aerosol tight angle 45 24 places max diameter 11 mm designed for 1 5 2 0 ml micro test tubes Spare lid aluminum for rotor FA 45 24 11 aerosol tight Fixed angle rotor FA 45 24
25. oneordde pue 0 4 1 6 Safety precautions and applicational limitations 32 2 Safety precautions and applicational limitations Transfer If the device is passed on to someone else please include the instruction manual Disposal In case the product is to be disposed of the relevant legal regulations are to be observed Information on the disposal of electrical and electronic devices in the European Community The disposal of electrical devices is regulated within the European Community by national regulations based on EU Directive 2002 96 EC on waste electrical and electronic equipment WEEE According to these regulations any devices supplied after 13 08 05 in the business to business sphere to which this product is assigned may no longer be disposed of in municipal or domestic waste They are marked with the following symbol to indicate this As disposal regulations within the EU may vary from country to country please contact your supplier if necessary 3 Operation 3 1 Functional and operator control elements See the frontal view Figure 1 2 on the first inside cover page of this manual menu enter Menu enter button see Section 3 8 Arrow button see Section 3 8 rpm rcf rpm rcf button see Section 3 10 time Time knob arrow buttons see Sections 3 6 3 7 and 3 9 speed Speed selector knob arrow see Sections 3 6 and 3 7 buttons open Lid release button see Section 3 6
26. ould be rinsed off thoroughly with water and lubricated with glycerin or talc after every clean to prevent them becoming brittle 4 2 Rotors Rotors need cleaning regularly to prevent residues of the material being centrifuged from changing their properties Check the rotors for residues and corrosion at least once a month This applies to the rotor bores in particular Please look after your rotor regularly this will protect it and increase its service life For thorough cleaning the rotor is unscrewed using the rotor key supplied and cleaned using a neutral agent e g Extran neutral RBS neutral Teepol 610 S Bacillol AF Meliseptol und Perform are recommended for cleaning and disinfecting the rotor and the rotor bores The rotor bores are also brushed out with a bottle brush The rotor and bores are then rinsed out thoroughly and placed on a cloth with the bores facing downwards to dry The rotor is then put back in and the rotor nut tightened up The rotors have been tested for resistance to the cleaning agents and disinfectants mentioned However this does not guarantee that the device is disinfected following application of one of the methods mentioned You should also consult your laboratory safety officer with regard to a suitable method of cleaning and disinfecting However before any cleaning or disinfecting method other than that recommended by the manufacturer is used please check with Eppen dorf that the intended metho
27. ous fixed angle rotors and adapters enable a large number of tubes like micro test tubes PCR strips Microtainers and Spin Columns to be used In the context of in vitro diagnostics the Centrifuge 5424 is designed among other things for chromosome analysis of human cells by the FISH technique Kievits et al 1990 Before using the centrifuge 5424 for the first time please read the manual The latest version of the manual and the safety instructions in your language can be found on the Internet at www eppendorf com You will see this symbol on your centrifuge and at a number of points throughout this manual The texts it highlights are relevant to safety Use the centrifuge only when you have read these safety instructions 1 1 Delivery package 1 Centrifuge 5424 1 Rotor FA 45 24 11 incl rotor lid only for order no 5424 000 410 5424 000 010 5424 000 428 5424 000 029 022620428 022620461 022620401 and 022620444 1 power cable 1 manual 1 rotor key 1 Captain Eppi rotor key holder 1 set of fuses 1 2 Installing the device AN To disconnect the power supply of the centrifuge from the power supply in the event of a fault an emergency switch must be provided away from the centrifuge preferably outside the room in which the centrifuge is located or next to the exit from this room Remove the transport safety device and keep it together with the centrifuge pack aging for possible use if the device is subsequently moved
28. rease the volume of this sound further use the arrow button to select the other items VOL2 to VOLS For every change a signal at the new volume is heard becoming louder each time menu enter confirms the selected volume of the loudspeaker and you automatically return to the item BACK in the first menu level Press the key again to exit the menu If the volume is not to be changed after all it is possible to exit the second menu level by select ing the BACK menu item and then confirming with the menu enter button 3 16 Standby mode If the centrifuge has not been used for 15 min it switches to standby mode The EP logo then appears in the display When a button or knob is used or the centrifuge lid is closed the centri fuge is reactivated and ready for operation Standby mode can be switched on and off via the menu menu enter activates the menu select the item SLEEP and confirm with In the second menu level select the item ON to switch Standby mode Standby mode can be switched off via the OFF item menu enter confirms that Standby mode has been switched on off and you automatically return to the item BACK in the first menu level Press the key again to exit the menu If standby mode is not to be changed after all it is possible to exit the second menu level by selecting the BACK menu item and then confirming with the menu enter button 3 Operation 3 17 Op
29. rom corrosion by the most common laboratory chemicals though this protection is not unlim ited Avoid damage from the use of aggressive chemicals such as strong and weak alkalis strong acids solutions of mercury copper and other heavy metal ions chlorinated hydro carbons concentrated salt solutions and phenol If the rotor is contaminated by aggressive substances clean it immediately with a neutral agent e g Extran neutral RBS neutral or Teepol 610 S This applies to the rotor bores in particular Rotors labeled coated are particularly resistant to chemicals However even on these rotors this does not replace regular cleaning as described in Section 4 Maintenance and cleaning Protect the rotors from mechanical damage Even minor scratches or cracks can result in serious internal material damage The material being centrifuged may not exceed a density of 1 2 g ml at maximum speed lf the rotor is run for a lengthy period or more often with short centrifugation runs the sam ple tubes will become hot Keep within the load limits specified for the tubes by the tube manufacturer Tubes may only be centrifuged at the preselected g force rcf if they are approved for this application by the manufacturer Before centrifugation all the tubes should be subjected to a visual inspection for material damage Damaged tubes must not be centrifuged as if tubes break there may be further damage to the centrifuge and its
30. short Short run button see Section 3 8 start stop Start stop button see Section 3 6 1 Power plug see Section 3 6 2 Power switch see Section 3 6 3 Rotor nut see Section 3 3 4 Rotor see Sections 3 3 to 3 5 E Emergency lid release see Section 3 17 3 2 Menu structure In the menu you can adjust the startup and braking ramp activate and deactivate the loudspeaker adjust run start vary the speed of short run centrifugation switch standby mode on and off control volume and set permanent centrifugation parameters menu enter activates the menu and confirms a selected menu item or adjusted setting the arrow button selects a menu item The menu consists of two levels M1 and M2 In the first menu level you can select between the various menu items The symbols for the functions light up In addition the symbol for each menu item selected flashes in the activated setting In the second menu level the setting for the selected function can be amended The relevant symbol always appears when the settings are selected The last inside cover page shows the menu structure with reference to the relevant sections of this manual 0 40 Operation 34 3 Operation 3 3 Fitting and removing the rotors Fit the rotor onto the motor shaft and tighten the rotor nut firmly by turning it clockwise using the appropriate rotor key provided Before each start check that the rotor is firmly tigh
31. site one another in pairs To ensure that the rotor is symmetrically loaded opposing tubes must contain the same filling volume 3 6 Centrifugation with timer setting Switch on the centrifuge with the power switch if necessary and open the lid using the open button The specified values of the last run are displayed Load the rotor symmetrically fit the rotor lid and close the centrifuge lid time adjusts the run time up to 10 min in 0 5 min increments then in 1 min increments up to 09 59 h speed adjusts the speed in increments of 50 rpm or the g force rcf in increments of 50 x 9 start stop starts centrifugation The symbol Bl flashes while the rotor is running During centrifugation the remaining run time is displayed in minutes The last minute is counted down in seconds In addition the speed of the rotor relevant g force rcf is displayed The timer setting speed and rpm rcf display can be adjusted during centrifugation The menu the open and the short buttons are blocked during centrifugation start stop if the button is pressed again centrifugation is stopped before expiry of the set run time After expiry of the set run time the centrifuge will otherwise stop automatically During braking the timer flashes and shows the spin time which has elapsed When the rotor has come to a standstill a signal tone is heard and the centrifuge lid opens automatically The display then shows the symbol 3 7 Adjusting centrif
32. splay shows the symbol of a o If the set parameters can be adjusted this is symbolized by ag If the parameters have been permanently set and these are to be changed using the knobs arrovv buttons SAFE appears in the display 3 14 Activating and deactivating the loudspeaker The loudspeaker is activated and deactivated via the menu menu enter activates the menu select the item ALARM and confirm with menu enter In the second menu level select the item ON to activate the loudspeaker The loudspeaker can be deactivated via the OFF item menu enter confirms that the loudspeaker has been activated deactivated and you automati cally return to the item BACK in the first menu level Press the key again to exit the menu If the setting for the loudspeaker is not to be changed after all it is possible to exit the second menu level by selecting the BACK menu item and then confirming with the menu enter button When the loudspeaker is activated the symbol d appears in the display 39 0 40 Operation 40 3 Operation 3 15 Volume control The volume of the loudspeaker is controlled in the menu The loudspeaker needs to be acti vated for you to hear the change in volume see Section 3 14 Activating and deactivating the loudspeaker menu enter activates the menu select the item VOL and confirm with menu enter In the second menu level select the item VOL1 A bleep is heard To inc
33. tened To release the rotor turn the rotor nut anticlockwise using the rotor key Under no circumstances centrifuge with rotors which already have obvious traces of corrosion or mechanical damage see Section 2 Safety precautions and applicational limitations 3 4 Loading the rotors N The rotors must only be loaded symmetrically The adapters must be loaded only with the specified tubes Minimize differences in weight between the filled sample tubes taring with a scale is recommended This will reduce wear on the drive and cut running noise The maximum load per bore is indicated on each rotor 3 5 Rotors A maximum of 24 x 1 5 2 0 ml micro test tubes can be centrifuged in rotors FA 45 24 11 and FA 45 24 11 Special With the appropriate adapters it is also possible to load it with 0 2 ml PCR tubes 0 4 ml micro test tubes 0 5 ml micro test tubes and 0 6 ml Microtainers It is also possible to load the rotor with 24 Spin Columns However the rotor F 45 18 11 Kit is recom mended for centrifuging Spin Columns The maximum speed of rotors FA 45 24 11 and FA 45 24 11 Special is 14 680 rpm 120 V 15 000 rpm and the maximum g force rcf 20 238 x g 120 V 21 130 x g The maximum load adapter tube and contents per bore for the rotors is 3 75 g AN Rotors FA 45 24 11 and FA 45 24 11 Special can be operated both vvith and vvithout a rotor lid VVithout a rotor lid the rotors are not aerosol tight and are slightly noisier Particu
34. u level Press the key again to exit the menu If the setting for run start is not to be changed after all it is possible to exit the second menu level by selecting the BACK menu item and then confirming with the menu enter button The selected setting is shown in the display If time is counted from the start the symbol appears If time is only counted from when the specified speed g force rcf is reached this is symbolized by Y 3 Operation 3 13 Permanent setting of parameters The selected centrifugation parameters can be permanently set via the menu so that neither time or rotation speed g force rcf can be altered using the relevant knobs arrow buttons menu enter activates the menu select the item LOCK and confirm with In the second menu level select the item ON if centrifugation parameters are to be made permanent To enable centrifugation parameters to be set freely select the item OFF using the arrow button menu enter confirms the permanent setting of parameters and you automatically return to the item BACK in the first menu level Press the key again to exit the menu If the permanent setting of parameters is not to be changed after all it is possible to exit the second menu level by selecting the BACK menu item and then confirming with the menu enter button The selected setting is shown in the display Once the centrifugation parameters are locked the di
35. uging parameters during centrifugation The timer setting and rotation speed can be changed during centrifugation using the two knobs or the arrow buttons If these parameters are adjusted the display begins flashing The new centrifuging parameters are accepted after a short time The time which has elapsed up to this point is offset against the new actual value In order that centrifugation cannot be stopped by changing the timer setting the shortest time which can be set is the time which has already elapsed plus 2 minutes Parameters cannot be adjusted during the braking process 35 0 40 Operation 36 3 Operation 3 8 Short spin centrifugation Switch on the centrifuge with the power switch if necessary and open the lid using the open button The specified values of the last run are displayed Load the rotor symmetrically fit the rotor lid and close the centrifuge lid short starts a short run at the specified speed The short button must be kept depressed throughout the entire short run The symbol ll flashes while the rotor is running Time is counted upwards in seconds Centrifugation is stopped by releasing the short button during the braking process centrifugation can be re started twice by pressing the short button again The timer flashes and shows the spin time which has elapsed during braking When the rotor has come to a standstill the centrifuge lid opens automatically The display then shows the
36. y cleaned Before using any cleaning or decontamination method other than that set out in Section 4 Maintenance and cleaning please consult Eppendorf to ensure the intended method will not damage the device Rotors and rotor lids must always be properly secured The centrifuge may only be operated with the rotor and rotor lid firmly tightened To do this before centrifugation place the rotor key supplied for tightening up the rotor on the rotor nut and turn it clockwise until the rotor nut is firmly tightened up The rotor lid is then screwed on tightly see Section 3 5 Rotors with regard to exceptions for rotors FA 45 24 11 and FA 45 24 11 Special for centrifuging micro test tubes with closed tube lids If unusual noises occur when the centrifuge starts the rotor or the rotor lid are not properly secure In this case stop centrifugation immediately using start stop The rotor may only be loaded symmetrically Opposing vessels should be of the same type and be filled equally On each rotor you will find information about maximum load adapter tube and contents per bore this limit may not be exceeded Rotors showing clear signs of corrosion or mechanical damage must not be used Check the accessories regularly 2 Safety precautions and applicational limitations Rotors are high grade components which have to withstand extreme stresses and strains Aluminum rotors are surface treated to provide them with a high level of protection f
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