Leica ST4040 - Leica Biosystems
Contents
1. 06 2009 Appendix 1 Staining Protocols for Leica ST4040 Linear Stainer Station no Elastica van Gieson Xylene Xylene Xylene 100 Alcohol 96 Alcohol 75 Alcohol Resorcinol G Resorcinol G Running water oO N OO FP UO N e co 10 Running water 11 Distilled water 12 Weigert iron haemalum 13 Weigert iron haemalum 14 25 HCI in 96 alcohol 15 Running water 16 Running water 17 Distilled water 18 van Gieson 19 van Gieson 20 96 Alcohol 21 96 Alcohol 22 100 Alcohol 23 100 Alcohol 24 100 Alcohol 25 Xylene 26 Xylene 27 Xylene Immersion time 1 Minute Draining time 5 Seconds Agitation ON Leica ST4040 Linear Stainer H amp E Xylene Xylene Xylene Xylene 100 Alcohol 100 Alcohol 96 Alcohol 75 Alcohol Running water Distilled water Haemalum Haemalum Running water Running water 25 HCI in water Running water 75 Alcohol Eosine Eosine 96 Alcohol 96 Alcohol 100 Alcohol 100 Alcohol 100 Alcohol Xylene Xylene Xylene 49 Appendix 2 Ordering Information 50 Lid cpl w handle Slide rack carrier Leica Slide rack carrier Medite Slide rack carrier Sakura Slide rack cpl Sakura slide rack CV5000 adapter stationary CV5000 adapter removable Storage container Activated carbon filter Suction tube 2m Suction tube 4m Tap water station assy Reagent station Optional load station right Optional load station left Optional unload station right2. Instruction Manual Leica ST4040 V1 4 Rev A English 06 2009 Always keep this manual near the instrument Read carefully prior to operating the instrument Leica ST4040 Linear Stainer etca MICROSYSTEMS IMPORTANT NOTE The information numerical data notes and val ue judgments contained in this manual repre sent the current state of scientific knowledge and state of the art technology as we under stand it following thorough Investigation in this field We are under no obligation to update the present manual periodically and on an ongoing basis according to the latest technical develop ments nor to provide our customers with addi tional copies updates etc of this manual For erroneous statements drawings technical illustrations etc contained in this manual we exclude liability as far as permissible according to the national legal system applicable in each individual case In particular no liability what soever is accepted for any financial loss or con sequential damage caused by or related to compliance with statements or other informa tion in this manual Statements drawings illustrations and other in formation as regards contents or technical de tails of the present manual are not to be consid ered as warranted characteristics of our prod Issued by Leica Biosystems Nussloch GmbH Heidelberger Str 17 19 D 69226 Nussloch Germany Phone 49 6224 143 0 Fax 49 6224 143 268 I
3. bers in drawings or to the drawing it Fig 5 seif Instrument type All information given in this instruction manual applies only to the instrument type indicated on the title page A name plate indicating the instrument serial number is attached to the back of the instru ment Required information for all enquiries For any enquiries please specify e Instrument type e Serial number Instruction Manual V1 4 06 2009 1 Important Information General This instruction manual includes important in structions and information related to the operat ing safety and maintenance of the instrument The instruction manual is an important part of the product It must be read carefully before first using the instrument and must always be kept with the instrument It will be necessary to add appropriate instruc tions to this instruction manual if imposed by existing national regulations or by laws on acci dent prevention and environmental protection in the country of the operating authority Read this instruction manual carefully before attempting to use or operate the instrument Please pay particular attention to the safety instructions in chapter 2 Please do read this information even if you are familiar with the operation and use A of Leica products Leica ST4040 Linear Stainer 1 2 Designated use The Leica ST4040 is an automated linear stainer for the preparation of histologica
4. see Chapter 6 8 3 commonly used with household appli ances 3 6 6 Leveling the instrument When installed in its final posi tion on the laboratory bench the instrument must be exactly level see also chap ter 3 1 Site requirements lf necessary the Instrument can be slightly readjusted via the adjustable instrument feet For that purpose screw the instrument feet in or out as needed until the instrument is positioned horizontally Instruction Manual V1 4 06 2009 3 6 7 Inserting the activated carbon filter If the instrument is not connected to a fume hood via exhaust air hose an activated carbon filter must be used To insert the activated carbon filter open the hinged front panel press the two locking pins which are located on the top right and left on the inside of the front panel a a F gt P be Remove the the activated carbon filter from the package Write the current date on the filter to be re minded to replace the filter in time Leica ST4040 Linear Stainer 3 Installation Insert the activated carbon filter Close the front panel While applying light pressure to the front panel press the two locking pins to lock the panel into place Plugging the power chord into the wall outlet Prior to plugging the power chord into
5. size10 station upwards using both your hands Leica ST4040 Linear Stainer 23 4 Installation of the Optional Load Unload Station s ON gt OFF e Lower the conveyor frame now connected to one single piece pushing the ON OFF but ton e Fit the connecting plates attached to the front and rear half of the conveyor frame of the optional load station over the insides of the front and rear half of conveyor frame of the basic instrument e Use the 4 hexagon socket screws which you have kept see step 1 to attach the lid of the optional load station to the corresponding hinge left hinge of basic instrument frames together with two hexagon socket the right side of the basic instrument screws hexagon key size 2 5 follow the same steps carried out lat erally reversed e Bolt the two front halves of the conveyor i To install an optional load station to e Bolt the two rear halves of the conveyor frames together with two hexagon socket screws hexagon key size 2 5 24 Instruction Manual V1 4 06 2009 4 Installation of the Optional Load Unload Station 4 4 2 Installing the optional unload station on the right of the basic instrument e Remove the 4 hexagon socket screws from the right lid hexagon key size 4 e Remove the lid Keep the screws will be needed later e Remove the 2 black screws from the right side panel
6. 2 different staining protocols though not run simultaneously can be run one directly after another without any delay whereas in a sin gle load model stations would have to be rearranged refilled etc prior to being able to run the second protocol Leica ST4040 Linear Stainer 31 6 Operation 6 4 Control panel functions IMMERSION TIME button Display select im mersion time DISPLAY SCREEN Display immersion and draining times buttons Change displayed parameters 32 DRAINING TIME button Display select draining time Agitation dips ON OFF PROGRAM 1 button Select program program no 1 PROGRAM 2 button Select program program no 2 AGITATION button ON OFF button switch on instrument gt stand by mode MAINS SWITCH connects disconnects instrument to from mains 0 OFF ON START STOP button Start stop staining programs Instruction Manual V 1 4 06 2009 6 Operation 6 5 Switching on mains and selecting direction of travel and alarm volume Switch on mains 0 OFF l ON The current software version Is displayed in 4 digits for 10 seconds e Only during this phase can the direction of travel and the alarm vol ume be selected and or changed lf direction of travel and or alarm volume have already been selected and you do not want to make any changes to those parameters eit
7. 3 3 Installing the instrument e To lift grip the instrument by the transport handles AN Two people are needed to lift carry the instrument since it weighs 73 kg see Technical data chapter 5 1 e Install the instrument on the designated bench e Unscrew the transport handles e Remove the plastic protective cover from the instrument e Check all delivered parts against the packing list to verify whether the delivery is complete see chapter 3 4 Standard delivery basic instrument and general accessories e For all further installation steps see chap ters 3 5 Electrical connection and 3 6 In Stalling the accessories 13 3 Installation 3 4 Standard delivery basic instrument and general accessories 3 4 1 Standard delivery 14 Basic instrument ST4040 single load model Basic instrument with three piece lid 27 Reagent stations plastic 4 tap water stations complete assy with inlet valve 1 Drain hose for waste water 2 m long 1 Tap water inlet hose 2 50 m long complete with 3 4 connection for water tap and replacement gasket 2 Lids for reagent stations 1 Cover for second row 1 Instruction manual woe cece cece csesesssesenseseesensenesseeeeees 14 0474 80001 1 Tool set including e 1 Single ended open jaw wrench size 27 e 1 Single ended open jaw wrench size 13 e 1 Screw driver 5 5 x 200 mm e 1 Screw driver 3 x 50 mm 1 Water tap ad
8. 6 5 Connecting the inlet hose to the tap uu ccc cccsccscscsssscsesesecsesesesecsssesesassesesessesesesaseesesassssesassesssassssesasassesesasaesesasassesesassesusesaeeees 18 So Over Leveling Me USE UIN MIN nce acces cannes cheese a ves ce eects sa nce case atcea ed aesicsacacaea oiesceiet guint vaeue gs tascam eco caees 18 3 6 7 Inserting the activated carbon filter rcv cccnacccerecs sascapnnssselycasincocwdiasccmeansnencaaadnssies ssianwssdeanenusseaewanbanseaaucsetnctdeantanssednuatoanaeasieacsemcadetacads 19 3 7 Plugging the power chord into E E P EAE E EPEN E P E E TE A E E E E E E A A the wall outlet 19 4 installation of the Optional Load Unload Station 0 00 0 cssssssssssssssesesesessseeseesanecseseessenecanacaeaesesesenesaeacaeaesesesenesanasaeaessesenesas 20 A EOE EN S aara E EE R cies eenee eee 20 4 2 Unpacking the optional load unload station ca cg cscce cece voeesserecesue tees ctece cece ste raceecocscoeeensestgeseocuccesesaasvoonemosesucssaeenaeoueeeton 20 4 2 1 Repacking the optional load unload SOMO EAI TE EEA E A EEE E E 20 4 3 Standard delivery and accessories optional load unload station oo ec ccccecscscsesscseseecscscsesscsesesecsesesecsssesesesssseeesessenees 21 4 3 1 Standard delivery optional load station 0 ccc ccccsssscscsesscscscsesscsesesecscscsessssesesesassesesassssesesassesesassssesesassesesassesesasaesececaesececaesees 21 Accessories ophonal load SLALOM esssS EE acca camieewehauctee 21 4 3 2 Standard deli
9. hexagon key size 3 Leica ST4040 Linear Stainer Remove the side panel from the side wall and detach the ground wire from the side wall Detach the ground wire and pull it out of the instrument will not be needed any longer Press the Start Stop button to move the conveyor frame to the upper position As soon as the conveyor frame has reached the upper end position switch the instrument off ON OFF button 25 4 Installation of the Optional Load Unload Station 26 Place the optional unload station on the right side of the instrument Move the optional unload station right up to the instrument inserting the two pins locat ed on the left and right at the bottom of the optional load station into the corresponding openings in the basic instrument Tighten the screw on the right wall of the op tional unload station ring wrench size10 Plate 1 which connects optional unload station and basic instument is fastened with two Allan screws In order to establish safe protective grounding it is essential to fasten plate 1 securely Pull the conveyor frame of the optional un load station upwards using both your hands Fit the connecting plates attached to the front and rear half of the conveyor frame of the optional unload station over the insides of the front and rear half
10. of the conveyor frame of the basic instrument Instruction Manual V1 4 06 2009 4 Installation of the Optional Load Unload Station e Bolt the two front halves of the conveyor Use the 4 hexagon socket screws which you frames together with two hexagon socket have kept see step 1 to attach the lid of the screws hexagon key size 2 5 optional unload station to the corresponding hinge right hinge on basic instrument e Bolt the two rear halves of the conveyor frames together with two hexagon socket screws hexagon key size 2 5 e Insert the plug of the unload sensor cable into the unload sensor port on the rear of the basic Instrument To install an optional unload station on the left side of the basic instrument follow the same steps carried out lat erally reversed e Lower the conveyor frame now connected to one single piece pushing the ON OFF button Leica ST4040 Linear Stainer 27 4 Installation of the Optional Load Unload Station 4 5 Changing the guide ramps for slide rack carriers To operate the instrument with Medite Hacker or Sakura slide racks the standard guide ramps no 1 for Leica slide racks must be exchanged for guide ramps no 2 see Standard deliv ery optional unload station chapter 4 3 2 e Place grooved ramp no 2 onto the rear pin and use the hexag
11. of the loading zone e Insert the slide rack carriers with the attached slide racks into the center of the stations Use the notches in the conveyor frame as orientation help e When operating the instrument with optional load unload sta tion insert the slide rack carriers into the reagent stations of the option al load station e When staining in two rows insert the slide racks into the stations of the loading zone as shown e When staining in two rows with optional load unload station at tached to the basic instrument insert the slide rack carriers into the reagent stations of the optional load station e To stain large slides individually use the adapter for large slides Leica ST4040 Linear Stainer 37 6 Operation 6 8 3 Starting the selected program e With the lid closed open the ball valve and if closed as well open the water tap If not only the ball valve but also the water tap has been closed gt e g when setting up the instrument the tap water flow rate has to be adjusted when starting a program see also chapter 3 6 e For that purpose press the Start Stop button e The water supply valves are opened e The processing mechanism starts e Via the ball valve slowly open or close further select the appropriate tap water flow rate look at the tap water station see which quantity of flow looks appropriate e The water has to rise inside the tap wate
12. outlet to the waste pipe To protect the user from hazardous solvent fumes make sure to operate the instrument either with the activated carbon filter or the exhaust air hose Instruction Manual V1 4 06 2009 2 Safety 2 1 2 Operating the instrument The instrument may only be operated by skilled personnel It may only be operated according to its designated use and in ac cordance with the instructions given in this manual While working with reagents filling emp ty ing the reagent stations working on the in strument while the lid s is are open appro priate protective gear lab coat gloves safe ty goggles must be worn Make sure to operate the instrument el ther with the activated carbon filter or with the exhaust air hose gt Chapter 3 6 Installing the accessories Even when the instrument is operated according to its designated use hazardous solvent fumes develop which are damaging to the operator s health and do also pose a risk of fire Risk of fire when working with an open flame Bunsen burner immediately next to the instrument solvent fumes Therefore keep a safety distance of 1 meter If Alarm off is selected not recommend ed the instrument must be observed con stantly to make sure all slide racks are re moved immediately when reaching the last station Leica ST4040 Linear Stainer If not only the water tap but also the ball valve has been closed gt
13. socket screws from the left lid hexagon key size 4 e Remove the lid Keep the screws will be needed later ST e Remove the 2 black screws from the left side panel hexagon key size 3 22 Remove the side panel from the side wall and detach the ground wire from the side wall Detach the ground wire and pull it out of the instrument will not be needed any longer Press the Start Stop button to move the conveyor frame to the upper position As soon as the conveyor frame has reached the upper end position switch the instrument off ON OFF button Instruction Manual V1 4 06 2009 4 Installation of the Optional Load Unload Station e Plate 1 which connects optional load station and basic instument is fastened with two Al lan screws In order to establish safe protective grounding it is essential to fasten plate 1 securely e Place the optional load station on the left side of the basic instrument e Move the optional load station right up to the ca instrument inserting the two pins located aS x on the left and right at the bottom of the op Vag E E A E tional load station into the corresponding E E openings in the basic instrument e Tighten the screw on the left wall of the op Pull the conveyor frame of the optional load tional load station ring wrench
14. the wall outlet check whether the mains switch is in OFF 0 position Connect the power chord to the wall outlet 19 4 Installation of the Optional Load Unload Station 4 1 Site requirements The installation site has to meet the following requirements e Stable laboratory bench exactly level at least 1 60 m wide and 60 cm deep for the ba sic instrument plus an additional 0 30 m of width for each of the optional stations e Other than that the same site requirements as listed for the basic instrument apply see Chapter 3 1 4 2 Unpacking the optional load unload station The unpacking instructions for all Leica ins truments are located in a transparent pro tective envelope on the outside of the instrument transportation crates 20 4 2 1 Repacking the optional load unload station We recommend keeping the original crates and packing material in store in case the load un load stations need to be repacked in the future The drawing below shows the design of the original crate and packing material The num bers indicate the sequence of disassembling and reassembling the crate Instruction Manual V1 4 06 2009 4 Installation of the Optional Load Unload Station 4 3 Standard delivery and accessories optional load unload station 4 3 1 Standard delivery optional load station e 1 Optional load station e 5 Reagent stations e 1 lid for reagent s
15. xylene or acetone For cleaning the instrument do not use any one of the following alcohol detergents containing alcohol window cleaner abra sive cleaning powders solvents containing xylene or acetone To clean the lids control panel and housing use mild household detergents see safety instruction above for non appropriate ingre dients When handling cleaning detergents follow the instructions of the manufacturer and make sure all applicable laboratory regula tlons are complied with When cleaning the instrument no liquid may enter in contact with any of the electrical connections or get into the interior of the in strument Wash the tap water and reagent stations in the dishwasher at a temperature of max 65 C Use a standard detergent for laboratory dishwashers At any rate avoid washing the stations at higher temperatures e g in industrial dis washers which run at a temperature of 85 C as the stations may become de formed Instruction Manual V1 4 06 2009 3 Installation 3 1 Site requirements The installation site has to meet the following requirements Stable laboratory bench exactly level at least 1 60 m wide and 60 cm deep Tap water supply at a distance of max 2 m and drain at a distance of max 1 50 m from the corresponding inlet and outlet at the back of the instrument Please bear in mind The connections are located at the extreme left on the back of th
16. 5 6 Operation a BEE I Prog 2 7 ae 6 8 Staining Draining time can be set from 0 seconds to 60 seconds Press the agitation button to activate or deactivate Agitation e LED illuminated Agitation activated e LED not illuminated Agitation deactivated Briefly press the same program button that was selected when you Startled programming here Prog 1 until the LED in the button lights up e The programmed parameters are stored To select and store parameters for program 2 proceed in identical fashion 6 8 1 Selecting a program O Prog Prog 2 Prog Frog 2 36 Select the desired program by pressing button Prog 1 or Prog 2 e Program selection Is only possible in stand by mode e LED in the program button lights up gt Program here Prog 1 is selected Instruction Manual V 1 4 06 2009 6 Operation 6 8 2 Preparing and inserting the slide racks e Insert the slides into the slide rack s e Attach the slide rack carrier to the slide rack s In addition to using Leica slide racks the Leica ST4040 can also be run with Medite Hacker and or Sakura slide racks For that pur pose the matching slide rack carriers for those brands have to be ordered see chapter 3 4 2 General accessories e Qpen the loading zone lid e Insert the first slide racks into the first stations
17. OFF button its e The fan goes on e The LED in the program button of the program used last lights up here Prog 1 and the programmed parameters are displayed Fi Z e Exception Prior to switching off the mains a program had not yet been fini shed or a power failure occurred while a program was in progress e n those cases the instrument does not switch to the stand by mode but continues the previously interrupted program 34 Instruction Manual V 1 4 06 2009 6 Operation 6 7 Programming i Programming must be done while in stand by mode The instrument e e Prog Prog 2 I x e Prog i a Leica ST4040 Linear Stainer can store 2 programs buttons Prog 1 and Prog 2 Select a program Prog 1 or Prog 2 Press the desired button here Prog 1 and hold for approx 5 seconds until the LED in the button starts flashing Press the Immersion time button The LED in the button lights up Use the buttons to select the desired value Immersion time can be set from 0 seconds up to 99 minutes 59 sec onds To set the time the button can be pressed and released going up or down step by step or it can be pressed and held lf the button is pressed and held the display scrolls faster Press the Draining time button The LED in the button lights up Use the buttons to select the desired value 3
18. Optional unload station left Lid for reagent stations opt load station Lid for unload container Unload container Tap water inlet hose Drain hose 4m Adapter for large slides 50 x 75 mm Disposable plastic film for control panel Power cord Australia Power cord EU Power cord USA CAN J Power cord UK Slow blowing fuse 6 3x32 T8 0A Slow blowing fuse 6 3x32 T0 6A Slow blowing fuse T1 6A 14 0474 32255 14 0474 32305 14 0474 32258 14 0474 32296 14 0474 32789 14 0474 33463 14 0474 32793 14 0474 32794 14 0474 32261 14 0474 32273 14 0422 31974 14 0422 31975 14 0474 32256 14 0474 32271 14 0474 32241 14 0474 32242 14 0474 32243 14 0474 32244 14 0474 33092 14 0474 33093 14 0474 32363 14 0474 32325 14 0474 33147 14 0456 27069 14 0474 33176 14 0411 32565 14 0411 13558 14 0411 13559 14 0411 27822 14 6943 08001 14 6943 00601 14 6943 01601 Instruction Manual V 1 4 06 2009
19. apter for 1 2 water taps 3 Disposable plastic films solvent resistant for control panel 1 Set of power chords e Euro e UK e USA Basic instrument T4040 double load model for double row staining i To use a single load model for double row staining order the appro priate number may vary depends on individual staining applica tions of the accessories listed below see chapter 3 4 2 General accessories for details Reagent stations Tap water stations Lids for reagent stations Slide racks Slide rack carriers Instruction Manual V 1 4 06 2009 3 Installation 3 4 2 General accessories Leica ST4040 Linear Stainer range of Leica ST4040 accessories Reagent stations plastic Tap water stations complete assy with inlet valve Leica slide racks metal Sakura slide racks plastic Adapter for large slides Drain hose 4m long Tap water inlet hose 2 50 m long complete with 3 4 connection for water tap Lids for reagent stations Slide rack carriers for Leica slide racks Slide rack carriers for Medite Hacker slide racks Slide rack carriers for Sakura slide racks Storage container for slide rack carriers fits on instrument housing Activated carbon filter Exhaust air hose 2 m long Exhaust air hose 4 m long Disposable plastic films solvent resistant for control panel set of 10 Optional load station left Optional load station right Optional unload station left Opti
20. ature of max 65 C Use a standard detergent for laboratory dishwashers At any rate avoid washing the stations at higher temperatures e g in industrial diswashers which run at a temperature of 85 C as the stations may become deformed e To clean the painted instrument surfaces and the lids use a mild household detergent see safety instructions on previous page for non appropriate ingredients e From time to time check the drain hose for accumulated dirt especially algae clean if necessary Leica ST4040 Linear Stainer 45 8 Cleaning and Maintenance 8 2 Maintenance 46 Only authorized Leica technical service engineers may open the in strument for maintenance and repair work Exception Changing the activated carbon filter this is the only maintenance task to be carried out by the user For your own safety never try to carry out any repairs yourself Any unauthorized repair whether carried out by the user or any third party not authorized by Leica will void the guarantee see also Chap ter 9 1 Warranty The Leica S14040 linear stainer is virtually maintenance free in operation However to ensure a smooth operation of the instrument over many years we recommend the following e Have the instrument inspected once per year by a qualified service en gineer authorized by Leica e Enter into a service contract at the end of the warranty period For more in
21. col Do not exceed the maximum volume or remain under the minimum volume Close the lid entirely Instruction Manual V 1 4 06 2009 6 Operation 6 3 Staining in two rows 6 3 1 Doubling the specimen throughput e For this type of application the sequences of reagent tap water sta tlons in rows 1 and 2 must be Identical e According to the station sequence selected for row 1 see Chapter 6 2 insert an identical sequence in row 2 e Both rows are run with the same program 6 3 2 Carrying out two different but matching staining protocols simultaneously e Precondition the program parameters of both protocols must be the same i e both rows are run with the same program e The two staining protocols are coordinated through the number of re agent stations and or the concentration or dilution of the reagents for details see Appendix1 Staining protocols Important note to chapters 6 3 1 and 6 3 2 e When staining in two rows standard slide rack carriers as for single row staining are used e Slide rack carriers located side by side in rows 1 and 2 are inserted laterally reversed into the same notch of the conveyor frame see left 6 3 3 Carrying out two different staining protocols two subsequent single loads e The two rows are never carried out simultaneously i e row 1 Is run with program 1 row 2 with program 2 or vice versa In 6 3 3 the advantage of staining in two rows Is that
22. e Instrument Fume hood at a distance of max 3 50 m from the instrument if the instrument is to be op erated with the exhaust air hose otherwise the instrument must be operated with the activated carbon filter Vibration free floor Sufficient open space 70 cm above the lab oratory bench to ensure trouble free open ing of the lids Stable ambient temperature of 10 C to 35 aC Relative air humidity of maximum 80 non condensing No other instruments which cause vibra tions installed nearby Leica ST4040 Linear Stainer Do not operate the instrument in rooms where risk of explosion exists Do not expose the instrument to direct sunlight windows Do not install the instrument above a heater 11 3 Installation 3 2 Unpacking the instrument The unpacking instructions for all Leica ins truments are located in a transparent pro tective envelope on the outside of the instru ment transportation crates 12 3 2 1 Repacking the instrument We recommend keeping the original crate and packing material in store in case the ins trument needs to be repacked in the future The drawing below shows the design of the original crate and packing material The num bers indicate the sequence of disassembling and reassembling the crate Instruction Manual V1 4 06 2009 3 Installation Leica ST4040 Linear Stainer
23. e g when setting up the instrument the tap water flow rate has to be adjusted when starting a program see also chapters 3 6 and 6 8 3 The flow rate must not be too fast to en sure that the specimens remain firmly at tached to the slide surface If a staining program is to be interrupted for an extended period of time do not leave any slide racks in the tap water sta tions in order to prevent them from drying out As soon as the alarm Is triggered immediate ly remove the completed slide rack from the last station or from the unload container of the optional unload station Otherwise im mersion times for the remaining slide racks will be prolonged In case of emergency switch off mains and unplug the power chord 2 Safety 2 1 3 Cleaning and maintenance 10 Only technical service engineers authorized by Leica may access the internal compo nents of the instrument for service and re pair Exception Changing the activated carbon fil ter is the only maintenance task to be car ried out by the user Prior to cleaning the instrument always switch off mains and unplug the power chord Dispose of used reagents according to the laboratory regulations in force in your coun try Spilled solvents reagents have to be wiped away immediately In case of long term ex posure the lid surfaces are only conditional ly resistant to solvents The painted surfaces and the control panel are not resistant to
24. eesseastereome 6 G2 MSHI Nee NS E E E A nal esas peace eeu aeeyesgnenateeneatanioerenes 7 2 S a E aadeoencea vaste uiuuuncenuuaaurtensneie oaemenaccadmade 8 2I AMET Mruchin cece ceccepesea tna ons cust oe caanesuce E RE 8 2 1 1 Transport and installation tien ccuteltacercrbay seperate a patatentesttcGheeabngebaeecuet ences cbs steelers ac acdsee le oer eateaeeceenmntenecedeen erie amends 8 Nee CATE Wea AVG TVS INU ELI VENN E eaen Ee E EE ae tecnyon desu EE EEEa 9 Alo Gleaming and mantenant tec rea S EEES 10 3 Msa a ON e EA E E E T a E E S 11 31 STS ELUM NS sssri a area ae ea come E aeo EEES katarda mere eee teen eee eae eee eed ees 11 32 Unpacking gt NE Ns UNE ea a E E bain ead eee cunsi onde setae Sel oeeecast a seaueuaade ueoeeseaietceeee 12 3 2 1 Repacking the instrument cccc cs cscsssscscscscsscscsesecscscsesssecsesecesscsessvavsesesassssesasassesesassesesassssesesassesesasassesesassesesasaesesasaesasesaeaeseeacsens 12 3 3 Installing the instrument ccc ccecscessesesscsssesececscsesesacsesssavsesesesessesasassssesassssevasassesesasasesasassesesassesesasassesesasaesesassesesasaesusecaeseseeacsees 13 3 4 Standard delivery basic instrument and general accessories oo ccsscsescscsssecscscsesscscsesecscsesesscsesesesassesessssesesesaesesececseseecaesees 14 Ae Samaa VEN cna en cere eer mer ttne eee trRrnn Serer erence A crn ere eae ae enna error 14 Basic Instruments 4040 single load model sii ec cs ceocsesaecnccnceessashvces
25. ent will not resume processing i e should you decide to remove only some of the completed slide racks from the optional unload sta tion at any rate make sure to clear the red ramps Outside the instrument the unload container with the corresponding lid see picture left can be used for intermediate storage of slide rack carriers prior to cover slipping see accessory list in chapter 4 3 2 40 Instruction Manual V 1 4 06 2009 6 Operation 6 11 Finishing work m e Once the last slide rack is removed press Start Stop STOP e Close the ball valve Do not change the setting of the water tap so that the adjusted tap water flow rate remains unaltered e fnecessary add or exchange reagents e Cover the stations with the station lids e Close the lids e Leave the fan on L e e Do not switch off the instrument via the ON OFF button or via the mains switch Leica ST4040 Linear Stainer 41 7 Trouble Shooting Problem No tap water supply Slide racks do not advance and or are not lowered into re agent tap water stations Waste water does not drain away 42 Possible cause Water tap closed Ball valve closed Magnetic valve or magnetic valve drive defective In house plumbing problem pipes clogged furred Slide racks slide rack car riers bent out of shape Slide rack carriers slide racks jammed Slide racks a
26. formation please contact your local Leica technical service center e Exchange the activated carbon filter regularly and dispose of it ac cording to the applicable laboratory rules in your country Instruction Manual V 1 4 06 2009 9 Warranty and Service Warranty Leica Biosystems Nussloch GmbH guarantees that the contractual prod uct delivered has been subjected to a comprehensive quality control pro cedure based on the Leica in house testing standards and that the prod uct is faultless and complies with all technical specifications and or agreed characteristics warranted The scope of the warranty is based on the content of the concluded agreement The warranty terms of your Leica sales organization or the or ganization from which you have purchased the contractual product shall apply exclusively Service information lf you require technical service or replacement parts please contact your Leica sales representative or dealer who sold the product Please provide the following information e Model name and serial number of the instrument e Location of the instrument and name of the person to contact e Reason for the service call e Date of delivery Decommissioning and disposal The instrument or parts of the instrument must be disposed of in compli ance with the local laws Leica ST4040 Linear Stainer 47 10 EC Declaration of Conformity CE feica MICROSYSTEMS EC Declaration of Confor
27. he tap water stations 3 6 2 Installing the drain hose e Connect the drain hose Upon installing the drain hose make sure there is a gradient from the drain outlet to the waste pipe A 3 6 3 Installing the exhaust air hose E 3j 2 Dee P a e i i e Connect the exhaust air hose optional i The instrument may be operated ei ther with the exhaust air hose or with the activated carbon filter 17 3 Installation 3 6 4 Placing the instrument in its permanent position e Place the instrument in its permanent posi tion on the laboratory bench e Connect the exhaust air hose to the fume hood or lead outside e Insert drain hose into drain 3 6 5 Connecting the inlet hose to the tap e Connecting to 3 4 water tap Connect the ball valve to the water tap and attach the tap water inlet hose to the ball valve e Connecting to 1 2 water tap Fit the adapter in between water tap and ball valve 18 When setting up the instrument an ad justment of the tap water flow rate has For safety reasons we recommend us ing a so called Aquastop device as Ball valve closed Do not yet open neither water tap nor ball valve see above to be done This adjustment cannot be done until after the tap water stations have been inserted For detailed in structions on the flow rate adjustment
28. her gt go on to Chapter 6 6 6 5 1 Selecting direction of travel START STOP C JG Be Leica ST4040 Linear Stainer Press Start Stop and hold Depending on the desired direction of travel press the or button Pressing the button Right half of display screen indication of seconds lights up gt Direction of travel to the right staining starts from the left Pressing the button Left half of display screen indication of minutes lights up gt Direction of travel to the left staining starts from the right 33 6 Operation 6 5 2 Selecting alarm volume e Press the Immersion time button and hold e Press or respectively e Each time when pressing a button the volume changes audibly e Alarm OFF gt Alarm low gt Alarm loud If Alarm OFF is selected not recommended the instrument must be observed constantly to make sure all slide racks are removed immedi ately when reaching the last station e Proceed to gt 6 6 1 Standby mode 6 6 Switching on mains without selecting direction of travel alarm volume i e Switch on mains 0 OFF I ON e The current software version Is displayed for 10 seconds g kd e fno further buttons are pressed during this phase after 10 sec OFF onds the LED of the ON OFF button lights up 6 6 1 Stand by mode oiri e Press ON
29. l and cytologi cal routine stainings It is designed for use in pathology laboratories and only for performing the following tasks e Staining of thin sections of tissue specimens or of cytological samples attached to micro scope slides Any use of the instrument other than its de signated use is considered improper 2 Safety 2 1 Safety instructions This instrument has been built and tested in ac cordance with the following safety regulations on electrical measuring control regulating and laboratory devices In order to maintain this condition and to ensure safe operation the operator must observe the instructions and warnings contained in this in struction manual For current information about applicable stan dards please refer to the CE declaration of con formity on our Internet site www histo solutions com 2 1 1 Transport and installation Do not operate the instrument in rooms where risk of explosion exists Do not expose the instrument to direct sun light windows Do not install the instrument above a heater Install the instrument on an even laboratory bench which must be absolutely level Two people are needed to lift carry the in strument Before connecting the instrument to mains make sure the correct voltage setting matching the nominal voltage at the site of installation has been selected Upon installing the drain hose make sure there is a gradient from the drain
30. lug the power chord Dispose of used reagents according to the laboratory regulations in force in your country Spilled solvents reagents have to be wiped away immediately In case of long term exposure the lid surfaces are only conditionally resistant to solvents The painted surfaces and the control panel are not resis tant to xy lene or acetone For cleaning the instrument do not use any one of the following al cohol detergents containing alcohol window cleaner abrasive cleaning powders solvents containing xylene or acetone To clean the lids control panel and housing use mild household de tergents see safety instruction above for non appropriate ingredi ents When handling cleaning detergents follow the instructions of the manufacturer and make sure all applicable laboratory regulations are complied with When cleaning the instrument no liquid may enter in contact with any of the electrical connections or get into the interior of the instru ment 44 Instruction Manual V 1 4 06 2009 8 Cleaning and Maintenance 8 1 Cleaning e Clean the reagent and tap water stations as well as the trough which holds the reagent and tape water stations regularly e For that purpose remove the reagent and tap water stations from the trough e The reagent and tap water stations can be washed in a laboratory dishwasher AN Wash the tap water and reagent stations in the dishwasher at a tem per
31. mity We herewith declare in exclusive responsibility that the Leica S1T4040 Linear stainer was developed designed and manufactured to conform with the e Directive 2006 95 EC of the European Parliament and of the Council Low Voltage e Directive 2004 108 EC of the European Parliament and of the Council electromagnetic compatibility e Directive 98 79 EC of the European Parliament and of the Council in vitro diagnostic medical devices The following harmonized standards were applied e EN 61010 1 2001 Safety requirements for electrical equipment for measurement control and laboratory use Part 1 General requirements e EN 61326 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements e DIN EN 61010 2 101 2002 Safety requirement for electrical equipment for measurement control and laboratory use Part 2 101 Particular requirements for in vitro diagnostic IVD e EN 14971 2007 Medical devices Application of risk management to medical devices e EN 591 2001 Instruction for use for in vitro diagnostic instruments for professional use In addition the following in house standards were applied e DIN EN ISO 9001 2000 Quality management systems Requirements Leica Biosystems Nussloch GmbH i Heidelberger Str 17 19 ne A tide ih tei MA EE D 69222 Nussloch Anne De Greef Saftt May 15 2008 President Biosystems Devision 48 Instruction Manual V 1 4
32. nd or slide rack carriers significantly bent out of shape or weld ing spots broken Toothed belt torn Motor or motor drive defec tive No or only insufficient gra dient of drain hose Drain of trough in basic in strument clogged up What to do Open water tap Open ball valve Call Technical Service Call in house technical ser vice plumber If bent only slightly read just carefully Reinsert correctly Parts not usable any more gt discard Call Technical Service Call Technical Service Install drain hose so there is a sufficient gradient from drain outlet on instrument to drain pipe Remove station and clean drain with fine bottle brush or similar Instruction Manual V1 4 06 2009 7 Trouble Shooting Problem Waste water does not drain away Fan does not work Controls do not work buttons do not respond no display indication Leica ST4040 Linear Stainer Possible cause Drain hose clogged up algae Fan or fan drive defective PCB defective Contacts loose Control panel drive defec tive What to do Clean drain hose see chapter 8 Cleaning Main tenance If necessary re place drain hose by new one Call Technical Service Call Technical Service Call Technical Service Call Technical Service 43 8 Cleaning and Maintenance 8 1 Cleaning Prior to cleaning the instrument always switch off mains and unp
33. ng gt Continue to follow instructions on this page e Insert the small screwdriver 3 x 50 mm into the notch at the lower end of the voltage selector shell Carefully release the lock using the screwdriver as a le ver Remove shell 2 with fuses 3 Take the fuses out of the shell Pull the voltage selector out of the shell and reinsert it such that the desired setting Is visible in the small window 1 of the shell Reinsert the shell together with voltage se lector and fuses into the corresponding opening at the back of the instrument and apply light pressure until it locks Double check if the correct setting now shows in window 1 Instruction Manual V1 4 06 2009 3 Installation 3 5 2 Connecting the power chord to the instrument a J e Remove the adhesive tape from the mains inlet e Select the appropriate power chord the in strument is delivered with several country specific power chords and connect it to the mains inlet at the rear of the instrument e Do not yet plug the power chord into the wall outlet e The unload sensor port remains empty un less the Instrument Is operated with optional unload station see Chapter 4 Leica ST4040 Linear Stainer 3 6 Installing the accessories 3 6 1 Installing the water inlet hose e Install the inlet hose which supplies water to t
34. nternet http www microsystems com Leica ST4040 Linear Stainer ucts These are determined only by the contract provisions agreed between ourselves and our customers Leica reserves the right to change technical specifications as well as manufacturing pro cesses without prior notice Only in this way Is it possible to continuously improve the technolo gy and manufacturing techniques used in our products This document is protected under copyright laws Any copyrights of this document are re tained by Leica Biosystems Nussloch GmbH Any reproduction of text and illustrations or of any parts thereof by means of print photocopy microfiche web cam or other methods includ ing any electronic systems and media re quires express prior permission in writing by Le ica Biosystems Nussloch GmbH For the instrument serial number and year of manufacture please refer to the name plate at the back of the instrument Leica Biosystems Nussloch GmbH Year of Manufacture asss Federal Republic of Germany Country of Origin Table of Contents IMPORTANT NOTE sce ziccasrnnts ccs nacuctstutce xe tesvspawassced cv cue svsnea svete oe nenshvaea ccs esatucaa ahteee Ea aa EANN EASA ANa aA aN ENN KATEN 3 1 Important DUNN OANA RENO assesses E eeeeeeee eee 6 VA Symbols used in this manual and 5c ctcect etd vosecnsteectnenacaedtaccedetnctitstaxassececnsssnscedebiecdelstetatenssodesessxenecatansecduiectdshaeesensecttenadaddanci
35. on key size 1 5 to fasten the two grub screws in the ramp clockwise rotation through about 180 e Place plain ramp no 2 onto the front pin and fasten the front ramp clockwise rotation of both grub screws through about 180 e Use the hexagon key size 1 5 to unscrew hexagon key size 1 5 both ramps no 1 loosening the two corre sponding grub screws in each ramp coun terclockwise rotation through about 180 i Store ramps no 1 for future use Remove both ramps 28 Instruction Manual V1 4 06 2009 5 1 Technical Data 5 Instrument Properties General Admissions VDE UL cUL C Tick Label Nominal supply voltage available 100VAC 10 120 VAC 10 230 VAC 10 240 V AC 10 Nominal frequency 50 60 Hz Maximum power draw 150 VA Protective class Mains fuses Circuit breaker manufactured by ETA 2A model 3120 F421 P711 W01D 2A Primary fuses manufactured by Schurter model Fst Secondary fuses manufactured by Schurter model Fst Pollution degree Overvoltage installation category Working temperature range 10 C to 35 C Relative air humidity Sound intensity level according to IEC 1010 UL 3101 EN 61010 Dimensions and weight Basic instrument W x H x D Basic instrument with optional load and unload station W x H x D Optional load unload station W x H x D Working height 318 mm Weight Basic instrument with accesso
36. onal unload station right Leica CV5000 adapter for slide racks e CV5000 slide rack adapter removable slides onto Leica slide racks for coverslipping in Leica CV5000 up to Leica CV5000 s nos lt CV026096 e CV5000 stationary slide rack adapter for permanent installation in Leica CV5000 for using Leica ST4040 slide racks in CV5000 starting from Leica CV5000 s nos gt C V026096 To order accessories please contact your Leica local sales organi zation for the latest literature current order numbers on the full For accessories for optional load unload station please refer to chapters 4 3 1 and or 4 3 2 respectively 15 3 Installation 3 5 Electrical connections 3 5 1 Adjusting the voltage selector A 16 The voltage selector is preset to match the nominal voltage of the country of delivery Nevertheless it is absolutely neces sary that you check the setting of the voltage selector prior to connecting the instrument to mains to make sure the setting is correct Connecting the instrument to mains with the voltage selector at a wrong setting can cause severe damage to the instrument Check the setting showing in window 1 Does it correspond to the nominal voltage in your laboratory If the setting is correct gt Go to next page chapter 3 5 2 lf the setting is not correct the voltage se lector must be changed to the correct setti
37. r station and the water flow has to be strong enough to wash surplus staining matter from the specimens Ball valve open However the flow rate must not be too fast to ensure that the speci mens remain firmly attached to the slide surface water tap fully open If there is no significant water pressure variation in your lab and as long as the number of tap water stations remains unchanged the set ting of the ball valve should not be altered To start end your daily routine simply open close the water tap i The ball valve is used to adjust the optimum water flow rate with the 38 Instruction Manual V 1 4 06 2009 6 Operation 6 8 4 Interrupting a program ae e fnecessary a staining program can be interrupted pressing the Start Stop button i The Leica ST4040 has a water saving function e Ifa program is interrupted Start Stop button the water supply to the tap water stations is automatically inter rupted e The remaining water in the tap water stations slowly drains away through a small opening in the bottom of the station e As soon as Start Stop is pressed again the program continues and the water supply to the tap water stations Is re stored If a staining program is to be interrupted for an extended period of time do not leave any slide racks in the tap water stations in order to prevent them from drying out e e Press Start Stop again
38. ries optional load station optional unload station Leica ST4040 Linear Stainer 2x I8A Motor F1 T 600 mA Electronic F2 11 6A 2 max 80 non condensing lt 70 dB 1 435 x 444 x 436 mm 1 969 x 444 x 436 mm 267 x 444 x 392 mm 73 kg 16 kg 14 kg 29 6 Operation 6 1 Setting up the instrument i To set up the instrument follow all instructions of chapter 6 step by step 6 2 Preparing the instrument for staining inserting and filling the stations 30 i The Leica ST4040 is equipped with a three piece lid thus e n Select a staining protocol For examples see Appendix 1 Staining protocols According to the selected staining protocol determine the order of re agent and tap water stations Open all three lids abling the operator to open just one of the two small lids on the right or left of the instrument for loading unloading slide racks This sys tem keeps the exposure of laboratory personnel to hazardous fumes to a minimum Remove the cover screws from those positions where you have decid ed to install tap water stations tap water stations can be installed from positions 3 through 20 Insert the tap water stations yellow see left and the reagent stations white Make sure that all stations are inserted correctly the stations must not be jammed overlapping rims Add reagents according to selected staining proto
39. s 34 ee A E E A E ee A A A A E ee 34 66I Stand 0y MOUE scinn E E o E 34 A E A E O 34 oL AONAN e A A 39 o o E a eccentrics ee eee 36 ae E BIN et PG NI E E E cts te ees a feted sei encased es ete E E E E dee setnnser E 36 SEEE AE EE E E maonomonosie1bi E E E A twee 36 6 8 2 Preparing and inserting the slide racks oo ccscscsssssscscscsesscscsesecscscsessssesesesesscsessvsssesesassesesassesesassssesesassesesasassesesasstsesasseseasaesaes 37 6 8 3 Starting the selected program eae Mennernee tt enn ene ner eee te Meee eee ne eerr nee ere ee Ree eee ene ree ee ne eee 38 Fs ae ej speek E A A ET Ga taser ann eaaIAPs 38 09 Removingihe Sde TACKS gen ener oO mT an E eer ee ee eee 39 6 8 4 Interrupting a program css carsch octet sarees tz emenecce cancer ccen ara annn ates ccaeescedtecesaesteraticedsacesasesacycassrpaiecs sein ara anapaateeeunesatesashastaectaniensttnctecestes 39 Foren EE E E D A E EE E peutic A AT N EEA E E A 39 691 Removing the lasitSlde Tat kK sionais E eee ee E ee ee tee 40 Ste EA T EO OE AE EP O TOE O OEE A O E EA A E E A A E E 40 6 10 Removing the slide racks in instruments equipped with optional unload station oo ccc ccceecsceseeecsssesecsssesecesseseeessesesees 40 ee ee ne ee te ee ee T ee ee eee 40 eM FMEA WING o E E E 41 A A va pigtac pa RIAG SANSA Meares eaaeaaaAda ea diate Aaaanaoraats 41 Js rouble Shooting scssi aaa n esate Aaaa S Eana aa EE aasia Aa Era ese raae ei 42 8 Cleaning and Maintenance scescsciececocetconaucysecincaua
40. serting and filling the stations 0 0 ccc csesscsesesscseseseeecsssesecsssesececseseececsesececsesees 30 69 Staining IN OWO TOWS recess cette eee cote ate ected E E E EE 31 6 3 1 Doubling the specimen throughput deekeeenwememnntenet entre ener woes estate ce came nenn io Nrent netstat or Mrteee omer yen rc mae ot re een cee mr 31 6 3 2 Carrying out two different but matching staining protocols simultaneously ou ec cecescsesetecscseeescsesesecsssesetesseseeecsesesees 31 6 3 3 Carrying out two different staining protocols two subsequent single loads ccccescccssssssseseecscssssseseseescsessesstsesesneneaeaeeees 31 oe S18 01d oy ore tLe AVL A HONS aaeeeaeeee eee een eee erent Mee nee eee Teese ae nn ree een ee ee ee een nee ene 32 6 5 Switching on mains and selecting direction of travel and alarm VOIUME s sesnsnsnsnssnsnninsnsirsnsnsinsrsnsnnsnsnnennrnnnnnnnnnrnrersnrerennn 33 A A A E E T RIAN eyes ad aeerE RAEI laN dea aaToante san 33 6 5 1 Selecting direction of travel scenccespnsoseasssiecescnscecnsneieec case basconniiescosntinadeaceessapencaanasunnesasenetad senda ssestesntcusansnsdsememuadeaahassgpmanensaasnan ekettnes 33 se AE EEEE E E ETN E E erence EAE EE AE E N TA E 33 0o72 Seca alarm VOlUME seca EE AEE EE AE 34 pete coos pbc TO A TE EEEE OET TAEA EE SETTE EE E TATEN I EEA E A 34 6 6 Switching on mains without selecting direction of travel alarm volume cccccccccsscsecsesessescsesccecsesesecessesecessesesecessesecacsesee
41. tations of optional load station e 1 Tool set including e 1 Hexagon key size 2 5 e 1 Hexagon key size 3 e 1 Hexagon key size 4 e 1 Ring wrench size 10 Accessories optional load station e Reagent stations e Lid for reagent stations of optional load station 4 3 2 Standard delivery optional unload station e 1 Optional unload station e 1 Unload container for optional unload station e 1Lid for unload container of optional unload station e 1 Connecting cable for unload sensor e 1 Pair of guide ramps no 2 for slide racks slide rack carriers by Medite Hacker and or Sakura e 1 Tool set including e 1 Hexagon key size 1 5 e 1 Hexagon key size 2 5 e 1 Hexagon key size 3 e 1 Hexagon key size 4 e 1 Ring wrench size 10 Accessories optional unload station e Unload container for optional unload station e Lid for unload container of optional unload station For double loading staining in two rows with optional load and or unload station attached to the basic instrument order the necessary accessories for the optional load and or unload station as needed i Can also be used for intermediate storage of slide racks prior to coverslipping see chapter 6 10 for details Leica ST4040 Linear Stainer 21 4 Installation of the Optional Load Unload Station 4 4 Installation 4 4 1 Installing the optional load station on the left side of the basic instrument e Remove the 4 hexagon
42. to continue the staining program START STOP 6 9 Removing the slide racks As soon as the alarm Is triggered immediately remove the completed slide rack from the last station or from the unload container of the op tional unload station Otherwise immersion times for the remaining slide racks will be prolonged e The Leica ST4040 is equipped with 2 unload sensors 1 sensor for each direction of travel located next to the outer container at each end of one row Leica ST4040 Linear Stainer 39 6 Operation e When the alarm is triggered immediately open the unloading zone lid and take out the slide rack e As soon as the slide rack has been removed the program continues e Close the unloading zone lid 6 9 1 Removing the last slide rack e First remove the slide rack e Then with the conveyor frame in the lowest position press Start Stop e This ends the program and shuts off the water valves 6 10 Removing the slide racks in instruments equipped with optional unload station e To remove the slide racks proceed as described above alarm Is trig gered remove the racks e Instruments equipped with optional unload station allow considerably more time before the slide racks have to be removed The unload station can hold 6 slide racks i e immediate rack removal is necessary only with every 6th rack As long as a slide rack carrier is located on the red ramps the instru m
43. usasrsnaeseasiecsasdatece sonssacau sensu haeaXadieusdses cu naguadeaweeeeseete 14 Basic instrument ST4040 double load model for double row Staining cccccsescsessssscsscseeescsscsescesesssessscsssetsesessnsesees 14 3 4 2 General accessories cccccscscsssssscscsesecscscsessscsesssssecsesssessesesesassesssassesesassssevasassesesassesesassesesasassesesassssesasassesesassesesasaesesesaeaesecaeseseeacsees 15 30 ENG UN hI SG MIM N a A E S E E E A AN 16 3 5 1 Adjusting the voltage selector scccscisecstccdstonncsertesSeedeasateaicitersestessecennesecaeuantsadsacsaisteaphitucbeascatedsacetepaeie lt ticiteemunneceensoemscegeaseeecisteren 16 3 5 2 Connecting the power chord to the ou ccscscsecscsescsccsescsesscscsesesassesesesassesesessesssessesesavassesasassesssasaesesesacsesasassesesasassesesassuesasseenes 17 JG Ursus aT Mags aCe SSO sic u EE ee ee ee eee re 17 3 6 1 Installing the water inlet hose en teceaceitcasectcucicerseue tears jasdecjesee okt oa locaton sausseere acted cabinbs00 soon uewtesatyettetne aac Goud adigortesensenerestatos cease Sstapeyonaees 17 JGA ITVS eM ihe drail MO tesa nceeey tarts cesta ce dca sucae R E 17 3 6 3 Installing the exhaust air OS GY ssasatsnese m sceceeasesce teaacs oanstosesecmebconcacdcecscasconvananstinveetuansasdeaekacsaueustsansusiaadietaeietectaneaee cetuauacnsspeedsanesesaxtags 17 Ae i ENET ee a ne ee em A A E A A ne eee T OANE ern ee Placing the instrument in its Gs ieee Rt on a a permanent position 3
44. very optional unload station oc cccscscsssscscscsesscsesesecscscsesscsesesessssesesassssesesassesesassesesesaesesescassesesasaesececsesecesaceees 21 4 Instruction Manual V 1 4 06 2009 Table of Contents Accessories optional unload station oo cece cscsecscscsescsesesecscscsessssesesesassesesassesesessesesesessssesesessesesaesesesasessesasaesasacacsesesacsesasaeass 21 aa SS AM tN rca tect va eaten iene A seceeeeceneaeeua 22 4 4 1 Installing the optional load Station occ cccsscscscsssscscscsecscscsesscscsesesecscsessvsssesesassesesassssesesassssesasassesesassesesaseesesasaesesacaesesesacaees 22 442 astaling the opionalunload SLALOM sesocsescteasecceeasiurecatucscs testdemaacesteatsusecaua te acaedeave vesaseaveciaieecasey aspdevcteanctace ile ssuccanaveeenviebeeges on the right of the basic instrument cccsccescsssesssecsesssecsesesesecsesevessesecesscsesasassesesassesesasassesesassssesassssesesaesasasacsesesasaesasasaesesaeaesesasass 25 45 Changing the guide ramps for slide rack carriers yi ssicccnenscecacesevessssssddaviel accessiateeceeedeasi dl Aa vieeeeansseieddielinl asec hemes 28 b5 nStument Properts siian ennea Pe rere ee cree ener ees cern eee re ee 29 MMII GN Ue tts deta te yon E S A doe eat ET E A E A E ETE ecole ts 29 6 0 01 100 eee reel artis A E co ears e ener freee ener ee aeneenre eee ne ere ener renee 30 61 PCT MUN EUV INS Me EM ce tart sta eecataen cece a E E 30 6 2 Preparing the instrument for staining in
45. yasgeuwencabeciacasctussesetctubsscusatevewievacsiesuncsstevusvaiuasvededecasasseuiutessinaesesoideuenssautatessasuenausates 44 BA GENG sigs lesen SSA atte st ne Sl set Ss eo enn enene aeons 44 B2 Meinten n qe S a eel cetera eerily pemciorialaceacas i 2 Warany and Sern ite crore E 47 1 Important Information The instruction manual for the Leica ST4040 includes chapters on the following subjects Chapter 1 Chapter 2 Chapter 3 Chapter 4 Chapter 5 Chapter 6 Chapter 7 Chapter 8 Chapter 9 Chapter 10 Appendix 1 Structure of this manual e Table of contents e mportant information on this manual Safety e Read this chapter before operating the instrument Installation e Unpacking and installation e Standard delivery accessory range Installation Optional load unload station e Unpacking and installation e Standard delivery accessory range Instrument features e Technical data Operation e Controls e Setup and daily use Trouble Shooting e Operating errors e Trouble shooting Cleaning and Maintenance Warranty and Service EC Declaration of Conformity Staining protocols Leica ST4040 1 1 Symbols used in this manual and their meaning Warnings appear in a grey box and are marked by a warning N triangle Notes i e important user information appear in a grey box and are marked by an in formation i symbol 5 Figures in brackets refer to item num
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