Home

3 - Frank`s Hospital Workshop

1. 25s ams S ptional Urine c Dec peer ad e gt Strip Reader External Printer npa NS A 2x5 Storage device Ext keyboard BB PS 2 Keyboard PS 2 Keyboard E Liquid Crystal Display m Pwe a Speaker e 1x4 2 Ly y i i x Control amp Measurement Board COMB E RBC BC chamber at chamber im a High Voltage B Board HVB a Amplifier Board eo o on board CCFL generator ceux Y AL j HGB Head f zig 7 EN cup Wo LEGEND n 2x5 10 pin Double Header Ribbon Cable Mn Tl LI A ee 2x10 m TTE q WL 1x4X 4 pin Single Header Ribbon Cable options X S shielded X P power X 2 2 wires only rev 1 09 96 Diatron MI PLC 10 4 Abacus tubing schematics ADS ARS NT e Y V JA Y rev 1 09 cAbacus Service Manual 97 10 5 Recommended kit of tools e PC standard keyboard PS 2 e Floppy drive with cables A154 J727 J7505 e Screwdrivers O Cross Slot Screwdrivers Philips S Slot Screwdrivers Hexagon Screwdrivers 3 5 2 5 2 0 1 5 mm sizes e Pocket digital multimeter e Diagonal Cutter plier e Nipper 10 6 Electronic schematics Electronic schematics can be found in the following Appendices in PDF Bowdmme Filename rev 1 09
2. BYTE length 1 path Measures Measure field_WBCH alias WM2 gt lt Field name GRAlo type BYTE length 1 path Measures Measure field_WBCH alias WM3 gt Field name Param type float length 23 path Measures Measure alias field_P incr 1 extID Param gt Field name Flag type BYTE length 23 path Measures Measure alias field_P alias2 _f incr 1 extID Flag gt Field name Warning type DWORD length 1 path Measures Measure alias field_WRN gt Field name Lyse type float length 1 path Measures Measure alias field_LYSE gt Field name Lyse_2 type float length 1 path Measures Measure alias field_LYSE2 gt Field name Options type WORD lengthz 1 path Measures Measure alias field_OPT extID Options gt Field name PrVMinW type WORD length 1 path Measures Measure alias PrVMinW gt Field name PrVMaxW type WORD length 1 path Measures Measure alias PrVMaxW gt Field name PrVMinR type WORD length 1 path Measures Measure alias PrVMinR gt Field name PrVMaxR type WORD length 1 path Measures Measure alias PrVMaxR gt Field name PrVMinW2 type WORD length 1 path Measures Measure alias PrVMinW2 gt Field name PrVMaxW2 type WORD length 1 path Measures Measure alias PrVMaxW2 gt Field name Age type BYTE length 1 path Measures Measure alias field_AGE gt lt Field name ClogReport type char length
3. Keep the ribbon cable far from the chamber interference with CPU fan or other digital logic traces CPU fan or other digital signal radiates to chamber or to the shielded amplifier cable Try keeping the ribbon cables far from the chamber and shielded cable 7 3 4 3 C Bad components or connections bad soldering salt residuals or component failure on amplifier especially if some reagent could get in the amplifier section Cleaning of PCB electrode socket or replacement of amplifier is required Check for the correct soldering of reference cable and its connector circuit board bad soldering or component failure Check the shielded cable connections as well Sometimes inside out connection hot electrode goes outside as a shield is the problem both ends of amplifier signal cable must be reversed analog signal ribbon cable it picks up noise Check the ribbon cable between the circuit board and the amplifier Maybe it is pinched under some screws or components This may cause trouble and even noise 7 3 4 4 D Pneumatic failures liquid paths that conduct noise into the chamber liquid remains under the chamber in drain tube during measurement the conducting liquid remains inside the drain tube making noise to appear there Check chamber draining path for clogging or salt crystals Check the pump operation Since draining of the chamber goes under pressure control maybe a bad pressure sensor or connection can cause troub
4. Patient alias Name gt lt Field name BirthDate type DateTime length 1 path Patient alias Birth gt Field name Sex type BYTE length 1 path Patient alias Sex extID Sex gt lt Field name Doctor type char length 16 path Measures Measure alias Doctor gt Field name LimitsLow type float length 23 path Measures Measure alias field_LimitLow incr 1 gt lt Field name LimitsHigh type float length 23 path Measures Measure alias field_LimitHigh incr 1 gt Field name PatTypeName type char length 20 path Measures Measure alias PatTypeName gt Field name VetMode type WORD length 1 path Measures Measure alias field VET source PatTypeName gt Field name HistoRBC type BYTE length 256 path Measures Measure field_RBCH alias D extID Histo gt Field name HistoPLT type BYTE length 256 path Measures Measure field_PLTH alias D extID Histo gt Field name HistoWBC type BYTE length 256 path Measures Measure field_WBCH alias D extID Histo gt lt Field name PLTlo type BYTE length 1 path Measures Measure field_PLTH alias PM1 gt Field name PLThi type BYTE length 1 path Measures Measure field_PLTH alias PM2 gt Field name RBClo type BYTE length 1 path Measures Measure field_RBCH alias RM1 gt Field name WBClo type BYTE length 1 path Measures Measure field_WBCH alias WM1 gt Field name LYMhi type
5. E7PROM board connecting CPU board and PPB 7 Motors with common opto board of needle moving motors H V 8 Main dilutor block with opto board for diluent lyse 9 Micro dilutor block with opto board for sampling 10 Valve boards set of 5 and max 7 11 Peristaltic Pump 12 Pressure Sensor 13 Digital Reagent Sensor Board 14 Graphic LCD Display Module with High Voltage Board 15 LCD and Keyboard controller and Keyboard Panel 16 Internal Printer Pneumatic and Power Board PPB Fluidic System Eo b cei ae y oi Re el aa msc HGB I F DEEP Ext Computer Ext Keyboard ES Power Board Interface S L1 5V 12V Pneum PC Motor controller driver 4425 a Motor controller driver 6 E Opto board of microdilutor Valyes icr T ira eee A rear Serial No CC Config Data DC DC converter 12V 12V analog power A D converter AMD Elan LCD VF Keyboard I F 12Vdc Ext Printer A ee A LZ kume m ER CR s ER ER CR 0 CR ER ER um CPU Board COMB START button Status LEDs Abacus Electronic Functional Block Diagram rev 1 09 10 Diatron MI PLC 2 1 1 Counting chambers with electrodes and measuring apertures Impedance method is used for determination of volume and number of cells In this method a known volume of dilution is drawn through a small aperture Constant current is passed through the aperture from one side to the other When
6. Each value is stored on 1 byte Actual Probe Voltage of given measurement can be calculated as PrVxxxx 50V 1024 Note 12 Age field interpretation Age unit Age gt 128 MONTH YEAR if Age unit MONTH Age of patient Age 128 this in MONTHS else Age of patient Age this in YEARS Note depending on database settings either BirthDate or Age gets filled LimitsLow LimitsHigh and Param match in their structure they contain values for parameters in the order below indices go from 0 to MaxParams 1 WBC RBC HGB HCT MCV MCH MCHC PLT PCT MPV PDWsd PDWcv PDW RDWsd RDWcv RDW LYM MID GRA LYMp MIDp GRAp RBCt WBCt WBCt2 Units can be found on page 4 note 3 Flags has the same order but contains values 0 5 each referring to the corresponding parameter Flags field value interpretation can be found on page 4 note 4 rev 1 09 _CAbacus Service Manual O O 10 3 Abacus cabling diagram Reagent Sensor Board Needle Moving Unit X Y e 9 2x5 59 Peristaltic Pump Valve Board 6 12 y lt gt p ap O Pressure Sensor Valve Board 1 5 Mi 141111 gt v p lt gt H y 1x4 3 f 1x4 3 did Micro Dilutor Pneumfatic amp Power Board PPB m sui DET n B Valve 0 a LE J Se a ax7 2 Amo Main td Lo OA e ei IRER Hr Prin 5V regulator 2 aM eic ne 1X4P fixed to chassis
7. HT O LFP PM1 lt HT gt 12 lt LF gt PM2 lt HT gt 204 lt LF gt RM1 lt HT gt 51 lt LF gt WM1 lt HT gt 23 lt LF gt WM2 lt HT gt 57 lt LF gt WM3 lt HT gt 92 lt LF gt PARN lt HT gt 22 lt LF gt POI lt HT gt 6 6 lt HT gt 0 lt LF gt P02 lt HT gt 4 29 lt HT gt 0 lt LF gt POSSHT gt L167 lt HAT gt O LE gt asa P21I lt HT gt 8 2 lt HT gt 0 lt bF gt P22 lt 8T gt Di 3o lt aAT gt 0 lt LE gt AGE lt HT gt 24 lt LF gt lt ETX gt 7C lt EOT gt A parameter called AGE is included in the header info This represents the age of the given patient if selected in service menu and defined at sample info screen The interpretation of this value is If the age value is greater than 128 then it represents months in the following way MONTHS AGE 128 months If itis less than 128 then it represents years This parameter has been removed in version 2 23 rev 1 09 cAbacus Service Manual 10 2 8 Serial Protocol 3 0 91 This version utilizes a new approach to record transmitting It sends the INIT package where instrument and PC should establish the communication After connection is established Abacus will send an X package with the XML file to give a description to the binary data sent in the F FULL package From then on F packages are sent and receiver software must interpret the binary data using the descriptors in the XML file Structures and data for interpreting F record without using an XML parser to get
8. Interrupt IRQ7 gt Watchdog Settings Mode Disabled e I O Chip Select I O Base Disabled Halt on Errors No e SECURITY A Fixed disk boot sector Normal Virus check reminder Disabled System backup reminder Disabled e BOOT Quick Boot mode Enabled gt Boot Device Priority CD ROM Drive Hard Drive DV XX Slave device FlashDisk SDTE XX Bootable Add in Cards Removable Devices Legacy Floppy Drives Network Boot rev 1 09 60 Diatron MI PLC 6 4 Checking the BIOS setup It is suggested to check it if the instrument hangs after switching on or software does not start or cannot be upgraded from floppy disk e Connect an external keyboard US layout to the instrument e Press F2 and keep pressed and switch the instrument on e Release F2 when the instrument beeps e Switch the instrument to MDA emulation mode by pressing i on the keypad e Check the key points listed above or from the enclosed Main Board Manual if you find differences set these settings e Go to the Exit menu upper right quarter of the display and select Exit Saving Changes Confirm this by selecting Yes in the pop up window e The instrument will restart with the new correct BIOS setup configurations 6 5 BlOS Description The DIMM PC 520 I is equipped with a JUMPtec Embedded BIOS which is located in a Flash EPROM onboard This device has an 8bit wide access Faster access is provided by the shadow RAM
9. Measurement cycle 2 4 Standby 2 5 Cleaning 2 6 Hard cleaning 2 Shutdown In the detailed process description figures the active tube is filled with black or gray color while an arrow 5 shows the direction of the flow Moving mechanic parts have another arrow indicating direction of movement In the section of the basic processes only relevant valves are mentioned in this section ON or OFF while all the other valves are in either ON or OFF state depending on the status of the instrument and other parallel running process Abacus employs a software waste full checking feature Software integrates volume of the reagents used rev 1 09 30 Diatron MI PLC 4 1 Basic processes 4 1 1 Sampling process The aspirating needle aspirates 25 ul 50 ul in prediluted mode of blood sample The Micro dilutor syringe makes the aspirating while the M5 Micro dilutor motor moves down ve Hor Gyr gt M Stepper Motor 2 way Valve 1K42 3 way Valve NE TP 1 3 Off Closed Off DILUENT LYSE CLEANER WASTE Dl iia 2 3 On Open On There is another sampling process for the second RBC dilution when 35 ul of primary dilution is aspirated from the MIX by the aspirating needle Both of the samples are separated from the diluent with a small air bubble and there is another air bubble between the sample and the end of the needle rev 1 09 cAbacus Service Manual 31 4 1 2 Needle washing process The instruments clean the
10. NAAA TA AAA D 6 SERVICE OPERATION 000 ia 58 6 1 OPENING THE INSTRUMENT as 58 6 2 MDA MONOCHROME DISPLAY ADAPTER EMULATION MODE uses sers secs secs cencecenneesenecenseceenssuns 58 6 3 KEY BIOS SETTINGS FOR CORRECT OPERATION ao 58 6 4 CHECKING THE BIOS ST Re de 60 6 5 BIOS DESCRIPTIONS Sn de adr do ed n ru EP een 60 6 6 DOS FUNCTIONS ON THE INSTRUMENT eoe doe ene ais 62 Ta SROUBLEBSHOOTING 55 2 2 2 VOI tane VY E v 00s da Rincer Vini ds 63 7 1 ERRORE E ene ree ee FD E E A A E E 64 Lal SOP LW AT C SVSICHE CLIONS alicatado 64 712 PREMA E CITOS eA beso Eds sve MU en Ne ik Na Ne iae dd 64 La MOGSUTOCLIONS A aa 65 7 1 4 PIENSE CUT ON Reise ittis veh desde di dessen tud est Me ten drones tite nue 66 dole LA ELO tette A TAAN IERE EN ndash een ad ed ect oe 67 T2 WARNING MESSAGES Garena aa aaa ed S 68 7 21 Pneuma C WAT OS SEA AAA RN 68 7232 Database Telarig WaT NOS etn ati aria 69 FPES Warnings relating to QC Measure aida 69 7 24 CAMDEN TE DUNE Wanin OS oes sass nie ANNE sn on team td 69 7 29 Hardware relating MONS een Re be Ne ose adu e has pep Ont ter dns tetes dui 70 726 Other WLIO S ai steele Soccer ever ino RON HU UE Ree ER ee AAA veo qeu dots dude desta a sedis 70 dads POSSIBLE CAUSES OF NOMS Benji nee uote oro o tation aor oro es ass gai Fe CONTAMINA TERESA enr nee ti se 71 7 92 BAd Cant CTOUNGIN RE TE ER ii 71 loded External electrical NOSO LR E A utes arts EE a 12 7 3 4 INTErRA ETE AT 282 ERROR ERO E OLI 72 Sa M
11. TIME lt HT gt 114500 lt LF gt PID lt HT gt 2 lt LF gt CHN lt HT gt 256 lt LF gt 9 lt HT gt 16 lt HT gt 26 lt HT gt 1 lt HT gt 0 lt ETX gt F2 lt EOT gt Receiver acknowledges RBC transmission and requests for WBC package lt ACK gt WC Abacus sends WBC package lt SOH gt DW lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt PID lt HT gt 2 lt LF gt CHN lt HT gt 256 lt LF gt 0 lt HT gt 15 lt HT gt 84 lt HT gt 5 lt HT gt 3 lt ETX gt 69 lt EOT gt Receiver acknowledges WBC transmission and closes the link lt ACK gt lt SPACE gt D It is possible to request for PLT histogram but it is not shown in this example rev 1 09 10 2 6 Serial Protocol 1 7 Receiver sends that it is ready to receive now lt ENQ gt Abacus sends acknowledge lt ACK gt Abacus sends INIT next time to initiate link there is data to send SOORLTAICSIXOCADSCUSTSHTOZ 227sHT 200II00SeHI l39521 2 501490 BOT Heceiver sends acknowledge lt ACK gt lt SPACE gt A Abacus sends DATA package lt SOH gt BD lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt SID lt HT gt 2 lt LF gt PID lt HT gt 26 lt LF gt NAME lt HT gt JOE SMITH lt LF gt MODE lt HT gt 0 lt LF gt WRN lt HT gt 0 lt LF gt PM1 lt HT gt 12 lt LF gt PM2 lt HT gt 204 lt LF gt RM1 lt HT gt 51 lt LF gt WM1 lt HT gt 23 lt LF gt WM2 lt HT gt 57 lt LF gt WM3 lt HT gt 92 lt LF gt PARN lt HT gt 22 l
12. Valve block Construction right side Valve block j 3d 1 i 4 27 pom Needle moving ca AMD mechanics AS p lame Aa ee Reagent o ra M y sensor gEJ f e Puffer reservior a ME O gt i Microdilutor Measuring chambers Pump Valve block Dilutor block rev 1 09 26 Diatron MI PLC Construction left side Perrin Pneumatic and S Power Board NEN I PPB a ID Eprom Board IDEPROM Control and Speaker Measurement Board COMB rev 1 09 e ee A 3 ADJUSTMENT Mechanical and hardware adjustments are described in this section Software settings are included in Section 5 2 3 1 Mechanical settings There are two important mechanical settings in the system e Opto wheel setting Vertical motor e Sampling needle setting The manufacturer adjusts the analyzer during production However in case of repairs in the mechanical system these adjustments should be checked The omission of these settings can cause malfunction or damages to the instrument 3 1 1 Opto wheel setting This setting is necessary for the vertical motor movements because this adjustment sets the opto end switches of the H amp V moving unit The top of this block is called HV head and it is shown in the figure below Set the distance to 1 2 mm between the moving Carriage and the stable part of the head Loose A screws to allow free movement of the timing belt Adjust the opto wh
13. and the support and between cogged wheels Checking and Lubricating Dilutor Piston Tips The cogged end of PTFE dilutor pistons should be cleaned and lubricated by neutral silicon grease A599 Apply just a thin layer and move it along the perimeter of the piston so that some of the material goes into the gaps between the sealing rings Repeat this step for lyse and dilutor pistons as well Check the condition of the micro piston sealing and replace if necessary Cleaning and Lubricating Needle Moving Mechanics The H amp V moving mechanics sliding bars should be cleaned from dust Lubricating of the sliding bars must be made using A598 Photolube oil containing PTFE Grease or pure lubricating oil is not suitable Checking and Replacing Washing Head Check the state of the washing head and replace if necessary After replacing washing head do not forget to perform correct adjustment of sampling needle height see Section 4 1 2 Checking and Replacing Peristaltic Pump Tube Replace peristaltic pump tube if needed You can check it by opening the lock and removing the tube for inspection Check for leakage of the tubing Reassemble the head rev 1 09 8 2 7 8 2 8 Warning Be careful DO NOT twist the tube while reposition it into the head because it will cause malfunction in a very short period of time Checking condition of lyse tubing Lyse reagent get the tubing material hard at connections air can be aspirated to
14. discriminator is in a wrong place in the PLT or RBC histogram then repeat the measurement for a correct PLT result RBC peak warning Perform cleaning and repeat the measurement clogging gt If itis a general problem change the RBC aperture RBC PLT The same action as in case of the M warning flag coincidence is too high Linearity error RBC PLT data The same action as in case of the D warning flag package errors RBC PLT time error The same action as in case of the D warning flag RBC PLT clogging The same action as in case of the C warning flag Table 10 Summary of warning flags related to RBC PLT rev 1 09 82 Diatron MI PLC 10 2 Serial Communication Protocol This document describes protocols 1 0 1 7 2 20 2 23 and 3 0 Only protocol version 3 0 contains sending Limits for parameters 10 2 1 General Description Abacus software is able to make serial connection link to a receiver device computer by connecting to the serial port of the instrument If Serial Communication Speed Utilities Settings Customize General Settings Baud Rate setting is set to a valid value other than Offline Abacus will try to initiate a communication sequence Abacus initiates serial communication the receiver must respond to this request The hardware protocol is 8 data bit 1 stop bit no parity If the other side is not responding within 1 second Abacus will repeat the transmission twice b
15. feature default For a detailed description of the BIOS Setup please refer to the section below The Setup Guide With the PhoenixBIOS Setup program it is possible to modify BIOS settings and control the special features of the computer The setup program uses a number of menus for making changes and turning the special features on or off General Information To start the PhoenixBlOS setup utility press F2 during the string Press lt F2 gt to enter setup is displayed during boot up The Main Menu will be displayed The Menu Bar The Menu Bar at the top of the window lists all the different menus Use the left right arrows to make a selection The Legend Bar Use the keys listed in the legend bar on the bottom to make your selection or exit the current menu The list below describes the legend keys and their alternates rev 1 09 cAbacus Service Manual 61 Key Function F1 or lt Alt H gt General help window lt Esc gt Exit this menu left or right Arrow key Select a different menu up or down Arrow key Move cursor up and down lt Tap gt or lt Shift Tap gt Cycle cursor up and down lt Home gt or lt End gt Move cursor to top or bottom of current window lt PgUp gt or lt PgDn gt Move cursor to next or previous page lt F5 gt or lt gt Select the previous value for the current field lt F6 gt or lt gt or lt Space gt Select the next value for the current field lt F9 gt Load default config
16. instrument software startup time can be increased Native keyboard Setting it to Yes will utilize the external PC keyboard with the language specific layout Calendar mode You can select between the Gregorian and the Jalaali Persian calendar All dates will be converted accordingly Operation Settings Patient data There are two options Birthdate and Age According to this setting both in the sample information dialog and in the database the instrument will prompt for the age or the date of birth If age is specified it can be given in years or months 5 2 6 3 Edit Service Code You can change here the default 6494 Enter ceroice enna service code to a maximum 12 character A seis alphanumeric code lt IS recommended not to change the code if it was changed it is Lonfirm HEW service codes hardly recommended to save the new code rev 1 09 5 2 7 Service Testing Menu Service Testing menu provides tools for checking hardware valve test 2 Motor test 3 Display test 4 Stress 5 Cleaning 5 cycles b Amplifier test 5 2 8 Valve Test Menu Valve test In the menu on the left you can see the valve numbers and a number under each eee alar vave P means peristatie pump Aaa Selected valve number is shown in inverse O is Off state 1 is On state Select valve lt LEFT gt RIGHT Set valve on UP off DOWN 088 8 mBar Use cursor keys for selecting and setting valve state or press
17. is a Service menu for servicing and operation checking purposes The entry point is in the User s Service screen where Service Information is displayed Enter the code to access Service menu 6484 5 2 2 Main Service Menu The Main Service menu provides access to submenus and service utilities IM Edit service contact 2 Device information 3 Calibration 4 Settings o Testing b Miscellaneous 5 2 3 Edit service contact Service information Here you can edit the Information card fields by cursor keys or by an external keyboard Press the OK button if a field is completed This information will appear in the User Service menu Service information Contact person ox e In the Device Information menu the model name Abacus the serial number the software version and 123456 compilation date appear ABP 1 6 ULTRAY 3 53 06 09 2006 rev 1 09 52 Diatron MI PLC 5 2 5 Service Calibration The analyzer provides a menu for Service calibration purposes In result calculations the service calibration factors are used as the user calibration factors so they are multiplied for each parameter RBCpisp Factrec user FaCtrec serv RBCneasured If the user factor is near the bound 0 80 1 20 by setting the corresponding service factor the user factor can be adjusted to 1 00 Example Fact rec user 1 19 and Fact rec serv 0 96 and Fact rec user 1 00 and Fact rec serv 1 14 gives the sam
18. to perform weekly washing head with a maintenance 5907 damp cloth Make sure it If you want to change the is clean then you proceed maintenance day contact Service Personnel rev 1 09 cAbacus Service Manual 74 7 3 Possible Causes of Noise Generally high count of any particle even if you think it should be low or near zero can be caused by NOISE i e something interferes with measurement The most important thing in these cases to identify the source of NOISE otherwise you cannot protect the system against it NOISE can come from has several sources and the different NOISE sources are added Sometimes we have to fight one of them but sometimes more Only one of them is enough to make problem 7 3 1 Contaminated reagent The most probable cause real particles are in the reagent and therefore the PLT blank is continuously high e g always 30 40 You can easily sort out this case by replacing diluent by opening a new tank PLT blank must go down is several blank measurements below 10 How can a good reagent become bad by time Ifthe reagent tube was contaminated and some bacteria begin to grow inside once you put an infected reagent tube into a new tank by time it can become infected as well i e the background PLT blank becomes high Wash the reagent tube which is in connection with the reagent with 196 of bleach solution then rinse with clean distilled water or diluent It can avoid the b
19. wo wan wow J 2 VALVES Valve connections N eeereeeeeeteee mi iu Pee PE M lll DETTE TT TETE i tec 4 NU p 12V_IN 2 Power input Smet PRINTER SORT EU 12 ie Internal Dur printer de connection JA ty Ve I PCB_CONNID ASE MA a board connection 5V voltage FLOPPY CD regulator Power to on chassis Main Dilutor connection PPB card contains the main power regulator circuits valve and motor driver circuits and other connections for the fluidic and pneumatic system s parts Power system generates 5V Digital power 8V Printer power and 12V Motor and valve power from the single 12V DC input signal Motor driver part consists of six separated PIC micro controllers with power drivers Horizontal Vertical and Sample rotor motors have one combined ribbon cable connection Main Dilutor with two motors and Micro dilutor have separated connectors Valve driver section is based on the valve driver PIC micro controller and three 8 bit powered output shift registers with built in protection diodes and there are two common ribbon cable connections for the 4 valve boards The peristaltic pump has a separated Darlington driver circuit for more reliable operation All the 7 6 for motors 1 for pneumatic microcontroller have 2 LEDs a yellow one and a green one The yellow one indicates motor moving or holding and active valve or pump moving it means current
20. 1000 Data file s is corrupt Stored data file is New stored data file will be new will be created all corrupt or missing created automatically by User stored measure data will Disk or software confirmation be lost error 1004 Error d on indexing file Fatal system or The same as in case of error YS program error 1000 7 1 2 Pneumatic errors Fatal pressure error Cannot make the Check the pump and the tubes 1100 Power off the system measuring vacuum fittings and valves around the ouffer reservoir Waste is full Waste container is Empty waste container Empty waste container 1 The system assumes that you will do it before going on Check Diluent container Diluent reagent con Replace the Diluent reagent tainer become empty container with a filled one or one of the diluent If this error still remains with a fluid sensor is too filled diluent reagent container sensitive or there are calibrate sensors and check the a lot of bubbles in the tubings and the fittings diluent tubes Check Lyse container Lyse reagent Replace the Lyse reagent containner become container with a filled one empty or the lyse If this error still remains with a fluid sensor is too filled lyse reagent container sensitive or there are calibrate sensors and check the lot of bubbles in the tubings and the fittings lyse tubes rev 1 09 cAbacus Service Manual 65 decreasing during and valves the
21. 29 path Measures Measure alias ClogReport gt lt Record gt lt Misc gt Field path Measures Measure alias Type value Haematology gt Field path Measures Measure alias field_PLTH value gt Field path Measures Measure field_ PLTH alias CHN value 256 gt Field path Measures Measure alias field_RBCH value gt Field path Measures Measure field_RBCH alias CHN value 256 gt Field path Measures Measure alias field_ WBCH value gt Field path Measures Measure field_ WBCH alias CHN value 256 gt Field path alias Version value 2 22 gt lt Field path Measures Measure alias Version value 2 22 gt Field path Measures Measure alias MeasureMode value 3 gt lt Misc gt lt MaxParams value 23 gt lt DB_VESRION value 2 22 gt lt DB_Struct_DAT gt Explanation of a Field of Record tag Record tag should be used to interpret F package lt Starting tag of field descriptor Field name RecNo Name of field can be used as reference in PC application tpe WORD Type descriptor of field length 1 How many bytes should be used for storing length T Technical tags gt Closing of field tag lt Field name LimitsLow type float length 23 path Measures Measure alias field_LimitLow incr 1 gt rev 1 09 cAbacus Service Manual 87 This field is an array contains 23 entries as indicated Interpretation of these en
22. 6 10 5 RECOMMENDED KIT OF TOOLS una lcs 97 OO LE CIRONIC SCHEMATICS ao 97 rev 1 09 Diatron MI PLC rev 1 09 cAbacus Service Manual 7 1 INTRODUCTION Although Abacus is a member of Diatron s Abacus junior instrument it has special and different characteristics compared to other junior family members We issue a dedicated Service Manual for this instrument information herein applies for Abacus To be well up in the instruments please read this manual carefully to have the knowledge for servicing the instruments perfectly and avoid extra costs and wasting precious time This Abacus Service Manual contains the functional descriptions of the analyzer operation of the fluidic systems adjustments and settings and very important information for the Service Personnel about the service operations and possible problems 1 1 Name and serial number Name Abacus Hematology Analyzer Serial No Every instrument has its own serial number which is printed on the rear panel label and it can be read out from Device Information or from the self test submenu This identity number is write protected by DIATRON 1 2 Intended use Abacus hematology analyzer is a fully automated cell counter for in vitro diagnostic use The compact instrument was developed for small clinics point of cares and vet offices Abacus can process 60 samples per hour and is intended to determine the following 18 hematology parameters from a 25ul wh
23. AINTENANCE zoeae ae eeaeee a Eaa aea Tap ea De ON Aa Fer cree epe eade ne sieste nd en deu E Spa sintio 74 8 1 WEEKEY MAINTENANCE BY USER sm ne nan rm int pb 74 8 1 1 Cleamnge the Wastin e edd aa 74 8 2 PERIODIC MAINTENANCE BY SERVICE nine 74 8 2 1 Check Selles and Device STAG TES AAA AAA AA 74 8 2 2 Cleaning and Greasing Dilutor BLOCK 0 74 8 2 3 Checking and Lubricaung Dilutor Piston TIOS datant ondes 74 8 2 4 Cleaning and Lubricating Needle Moving Mechanics eese nennen 74 8 2 5 Checking and Replacing Washing Lead dai denses 74 8 2 6 Checking and Replacing Peristaltic Pump Tube cesses eene 74 8 2 7 CHECKING condition OF ANS CUDASS A A NE EN 75 6 2 6 bleaching of TAUSTE ds ed oth I E auem 75 ds SPARE PARIS 76 cAbacus Service Manual 5 10 APPENDICES RS Re 80 WOA WARNING BACS nn Anse cn et aie dt DA Een atin ne lai ee a d tics ara vnd Pr m 80 02 SERIAL COMMUNICATION PROTOCOL siii did 82 10 2 1 GCI GDS CON edens E A AE NS 82 10 2 2 Format or Packages Sent sonet itn nero utet ne ner te 83 10 2 3 Format of Acknowledge of the Receiver esse 83 10 24 Detailed Description Of Packages e s eee rm nn 83 10 2 5 Serial UU IBN RT 10 2 6 Seria PFOLOCOD T I e aaa diosas 59 10 2 7 Serial Protocol 2 20 2 29 assortie rater tir ae teen tue 90 10 2 6 SrA TOO COLS DA A E A E EE 9 0 3 ABACUSECABLINGDIAGRAM tiara avia 95 LOA ABACUSETUBING SCHEMATICS ii AA A RA Aa de eaves 9
24. C 2 1 13 Keypad The analyzer has a 29 button foil keypad including numerical keypad 0 9 cursor moving OK and Del buttons and 6 6 function buttons above and under the LCD display as itis shown in the picture below Function buttons Status LED Cursor buttons 2 1 14 External Power Supply The analyzer works with an external power supply The next figure shows the power supply unit generating 12VDC 115V or 230V AC inlet 12V DC outlet The power supply modules have an auto range input which makes possible to use them with 230V or 115V mains outlet and it has the CE and UL safety certification The input socket of the power supply is a standard 3 terminal plug with power cable connection the output is a special lockable socket as it is shown in the picture rev 1 09 2 2 Main mechanic and fluidic parts of the Analyzer Abacus Hematology Analyzers consist of the following mechanic and fluidic parts 1 Washing head H amp V moving unit Micro Dilutor Dilutor Chambers Cell counter probes Puffer reservoir 9 CON 0 Eh W D Sampling needle Pump 10 Valves 11 Tubing cAbacus Fluidic Schematics Version 1 3 2006 10 31 h amp cm ve yr m un stepper Motor IN 3 way Valve x 2 way Valve 1 3 Off Closed Off DILUENT LYSE CLEANER WASTE vias m ird ce oui rev 1 09 20 Diatron MI PLC 2 2 1 Sampling needle Sampling needle is assembled in t
25. Ctime sec P22 WBCtime sec The parameter value is always 4 characters wide spaces from left added if necessary It can be 9999 if the value could not be displayed in 4 digits or if the value could not be calculated because of an error The meaning of the parameter flags Fiag Displayed Meaning su Value high more than upper limit Value low less than lower limit Value is unreliable 3 Valueisunreliable 4 E Value not given because of error Value is 5 Value cannot be calculated There is no value RBC WBC and PLT package The format of the histogram packages are the same they differ in type descriptor only The package contains the sample data to identify the package and the histogram SNO lt HT gt 152 lt LF gt internal identifier DATE lt HT gt 19980715 lt LF gt date of measurement YYYYMMDD TIME lt HT gt 114500 lt LF gt time of measurement HHMMSS PID lt HT gt 2 lt LF gt patient identifier 0 9999 CHN lt HT gt 256 lt LF gt number of histogram channels 256 9 lt HT gt value of first histogram channel 1 lt HT gt value of 255 histogram channel O value of the last histogram channel rev 1 09 86 Diatron MI PLC XML record descriptor package Each time a communication is initialized Abacus will send an XML file in ASCII mode A receiver application should be aware of this by saving this XML file for later use This package contains the description of the
26. FULL record format FULL record format means transmission of a record stored in Abacus as is in binary format Data sent in an F package should be interpreted according to the description in the XML file below Would an F package arrive without an X package received earlier receiver application can ask for the XML descriptor by sending a request for an X package See WBC RBC PLT histogram package request XML file sent by Abacus DB Struct DAT Header Field name Head type char lengih 9 gt Field namez Modified type Date Time length 1 gt Field namez Length type DWORD length 1 gt Field name Order type BYTE length 1 gt Field name Dummy1 type BYTE length 1 gt Field name Dummy2 type WORD length 1 gt Field name Capacity type WORD length 1 gt Field name RecLen type WORD length 1 gt Field name Dummy3 type BYTE length 7 gt lt Header gt lt Record gt Field name RecNo type WORD length 1 path Measures Measure alias RecNo gt lt Field name TimeStamp type DateTime length 1 path Measures Measure alias Date gt Field name OperatorlD type WORD length 1 path Measures Measure alias OplD gt Field name SamplelD type char length 8 path Measures Measure alias SID gt Field name PatientlD type char length 20 path Patient alias PID gt Field name Name type char length 32 path
27. Interpretation of the binary information should happen using field definitions of the XML file received in an X package SOH MESSAGE ID gt F lt STX gt lt SIZE1 gt lt SIZE2 gt lt PAYLOAD actual binary record SIZE1 256 SIZE2 BYTES lt ETX gt lt CHKSUM1 gt lt CHKSUM2 gt lt EOT gt rev 1 09 88 Diatron MI PLC 10 2 5 Serial Protocol 1 0 Receiver sends that it is ready to receive now lt ENQ gt Abacus sends acknowledge lt ACK gt Abacus sends INIT to initiate link there is data to send SOQITAISSTX2ADSCUSTSETLO HT EZOOITODS HT TI2502TS8BTX230SBOT Receiver sends acknowledge lt ACK gt lt SPACE gt A Abacus sends DATA package lt SOH gt BD lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt PID lt HT gt 2 lt LF gt NAME lt HT gt JOE SMITH lt LF gt MODE lt HT gt O lt LF gt WRN lt HT gt O lt LF gt PM1 lt HT gt 12 lt LF gt PM2 lt HT gt 204 lt LF gt RM1 lt HT gt 51 lt LF gt WM1 lt HT gt 23 lt LF gt WM2 lt HT gt 57 lt LF gt WM3 lt HT gt 92 lt LF gt PARN lt HT gt 22 lt LF gt PO1 lt HT gt 6 6 lt HT gt 0 lt LF gt P02 lt HT gt 4 29 lt HT gt 0 lt LF gt POSSHT 16 lt HT gt 0 lt LF gt 12 BPAZLUSHIO 8 2 lt HT gt 0SLE gt P22 lt HT gt 5 3 lt HT gt 0 lt LF gt SETXCICSBOTS Receiver acknowledges DATA transmission and requests for RBC package lt ACK gt RB Abacus sends RBC package lt SOH gt CR lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt
28. OK to toggle state Toggle valves lt UK gt 5 2 9 Motor Test Menu Pressing numeric key on keyboard the Use number keys to activate option regarding motors start operation repeatedly PUTATE until pressing 1 or another key All motors 1 Mone EMEN stop at initial positions b Micro rev 1 09 56 Diatron MI PLC 5 2 10 Display and Keyboard Test Display and keyboard test is provided to check keypad and LCD panel Press a key to test and the LCD will show the Last pressed key code of the pressed key and will be invert the colors at each touch of a key for testing that every LCD dot is functioning norme 5 2 11 Stress Mode In Stress mode the instrument performs measuring cycles without sample blank measurements continuously This can be used for burn in tests or to check pneumatic system after changing any main fluidic parts You can have information about stability cleanliness HGB operation and counting time stability Results of the last 10 PLT and HGB blank is displayed as well You can detect any kind of noise or bubbles in the system if the PLT is not stable low or HGB has big variation To exit from this mode press the START button at the end of a normal cycle until the Stress operation is finished rev 1 09 5 2 12 Miscellaneous Settings Miscellaneous In this menu you can directly perform software upgrade Software upgrade this will restart the Clear device statistics instru
29. P140 AP250 AP251 AP202 9210 J214 14P ribbon cable microdilutor 26P ribbon cable dilutor 3P ribbon cable start button 10P ribbon cable HUR Amplifier board complete Dilutor block complete Lyse syringe with piston Lyse syringe Bearing for dilutor Dilutor panel mounted rev 1 09 19 80 Diatron MI PLC 10 APPENDICES 10 1 Warning flags In the followings we summarize the warning flags and give an explanation of their possible cause and a few hints to overcome the problem Uppercase letters refer to WBC or HGB problems Flag Meaning Recommended user action W WBC three part gt Repeat the measurement Possibly lyse problem warning or WBC gt Check the discriminators in the WBC histogram If the three part diff discriminators are in the proper place the populations can unsuccessful be separated by eye then the results can be accepted dass gt Possibly lyse problem but in some pathological samples too high lymphocytes it can be happened EHAE Brk peeso pero ouentpeoen o o or no HGB blank Possibly lyse or diluent problem T oo bark Porc pon ore o o O or no WBC blank gt Possibly lyse or diluent problem L WBC RBC limit Check the 1 RBC LYM discriminator If it is in the minimum warning point or close to it accept the results Otherwise repeat the measurement gt f the retried action makes very similar results and the discriminator is in a wrong plac
30. PPB rev 1 09 16 Diatron MI PLC 2 1 10 Pressure Sensor This is an MPX5100AP calibrated pressure sensor which can measure the required air pressure and vacuum The Pressure Sensor is connected directly to the PPB card Connection to Puffer reservoir Connection to PPB The pressure sensor can operate from 5V only It is a calibrated sensor with 0 1 1 Bar input range Do not apply more than 1 5 Bar to it because it can ruin the pressure sensor 2 1 11 Digital Reagent Sensor Board This board contains four liquid detector opto detectors optos and a reference opto for automatic temperature and stray light compensation The reference opto is located in the middle and it has the same temperature and backlight conditions as the sensing ones Heference detector Control LEDs Reagent detectors The Reagent Sensor Board is connected to the PPB card and the valve driver micro controller makes the sensing and compensating operations Instrument makes automatic initialization called calibration of reagent sensors during priming phase of fluidics rev 1 09 Abacus Service Manual 17 2 1 12 LCD Display Module with High Voltage Board Ea ei SERERE m LCD amp Keypad LCD module controller Start key connection and OS status LED om o es LCD backlight lamp a Keypad connector m Display assembly contains the 240x128 dots graphics LCD di
31. T gt 23 lt LF gt WBC upper marker WM2 lt HT gt 57 lt LF gt WBC LYM MID marker WM3 lt HT gt 92 lt LF gt WBC MID GRA marker PARN lt HT gt 22 lt LF gt number of parameters to send PO1 lt HT gt 6 6 lt HT gt 0 lt LF gt first parameter ID value and flag PO2 lt HT gt 4 29 lt HT gt 0 lt LF gt second parameter ID value and flag P22 HT 8 2 lt HT gt 0 lt LF gt last parameter ID value and flag Remarks Position and meaning of the warning flags Letter 0x00001 RBC PLT clogging WES 0x00008 RBC PLT Coincidence is too high Linearity error 0x00040 NN RBC Blank is high or no RBC blank uL 0x00080 EE PLT blank is high or no PLT blank 8 0x00100 C Q WBC clogging 11 0x00800 M N WBC coincidence is too high Linearity error 0x04000 EN WBC Blank is high or no WBC blank 0x08000 HGB Blank is high or no HGB blank 0x10000 No WBC three part The detailed description can be found in the User s Manual rev 1 09 The markers are given in histogram channel between 0 and 255 where 0 means that the marker could not be found The parameter IDs in Abacus are P01 WBC 10 1 P02 RBC 10 I PO3 HGB g l P04 HCT 96 PO5 MCV fl PO6 MCH pg PO7 MCHC g l PO8 PLT 10 D PO9 PCT 96 P10 MPV fl P11 PDWsd fl P12 PDWcv 96 P13 RDWsd fl P14 RDWcv 96 P15 LYM 10 l P16 MON 10 I P17 GRA 10 l P18 LYM 96 P19 MON P20 GRA P21 RB
32. WBC chamber draining is performed via V3 On and V4 On valves by the pump while the V5 must be off The WBC chamber draining is always executed without using the puffer reservoir So other tasks like RBC chamber draining can be performed parallel with this operation 4 4 v12 LE 42 RBC MIX Ver MD X vo Hor Se X X va gt V4 2 X 1 g PS 8 Pump A M Stepper Motor 4 2 3 way Valve 2 way Valve 1 3 Off EF Closed Off DILUENT LYSE CLEANER WASTE Te WT 23 0n Open On rev 1 09 38 Diatron MI PLC 4 1 9 RBC chamber draining process The RBC chamber is drained by the vacuum in the puffer reservoir through V1 On valve V2 must be off This vacuum is made and maintenated by the peristaltic pump During the draining the pump can perform either other task like WBC chamber draining or needle wash or can help the draining of the RBC chamber Stepper Motor IN Az 3 way Valve 2 way Valve g 4x XP Closed Off DILUENT LYSE CLEANER WASTE Liquid Detsctor 2 3 On Open On rev 1 09 Se 4 1 10 Cleaner priming process The cleaner solution is primed by vacuum in the following way V7 On Cleaner Detector V6 On back side of the RBC aperture back side of the WBC aperture V2 On V3 On V4 On and the peristaltic pump The V1 and V5 valves are off The flow of the cleaner is controlled by the vacuum generated in the puffer reservoir before the start o
33. a cell passes through the aperture it causes a change in resistance which generates a voltage pulse The amplitude of the voltage pulse is proportional to the ratio of cell volume per aperture volume This is used to determine the volume of cells The number of cells can be obtained by counting the pulses In the instrument there are two Cellcounter Probes WBC probe with 100 um aperture and RBC probe with 80 um aperture Both have a ground electrode assembly and U shaped metal fixing as it is shown in the next figure Assembled Cell counter Probe Measuring tubes Les Red rings mark measuring tubes One RBC 80 um Es LE em Two WBC 100 um ues WE C O ring U shaped metal fixing Measuring tube Reference electrode The aperture is made of ruby and it is moulded into the measuring tube 2 1 2 HGB Head Hemoglobin head is placed on the two sides of the WBC chamber It contains light source LED at 540 nm wavelength and Photo Detector TSL235 The Photo Detector converts the light to frequency The HGB concentration is a logarithmic function of this frequency measured by the FPGA circuit of the COMB card Connection to the amplifier TSL235 rev 1 09 AAA CC The analyzer performs enhanced Hemoglobin measurement technology for HGB measurement The output of HGB head is frequency TSL235 detector is light to frequency converter This signal is counted by a digital counter
34. acteria to grow inside l tank is open and cap is not installed or closed external dust can make reagent dirty 7 3 2 Bad earth grounding In this case external ground referenced noise can get into the system by ground coupling If system ground is not good enough ground terminal can become a noise source as well i e external signals will be coupled into the system instead of protecting it If no earth ground is available you can use a screw at the rear panel to connect a ground potential to the case so that noise immunity can be increased Measure voltage on ground terminal to make sure earth grounding is correct AC voltage lower than 1V is accepted in this case At some places as a bad practice electricians like to connect earth ground terminal to neutral wire Depending on the resistance of the neutral back wire where it is really earthed several volts can appear and this way any inductive noise will be coupled into the instrument It is better to create a real earth grounding and connecting it to the rear screw rev 1 09 72 Diatron MI PLC 7 3 3 External electrical noise If another instrument is near the analyzer can radiate electromagnetic signals in the 1 kHz 100 kHz frequency region it can be picked up by the system especially if they are very close to each other or the grounding is not quite perfect You can easily identify this noise source by relocating the instrument noise high PLT blan
35. al keyboard to be connected Hold the i button and press and release the button on the foil keyboard of the analyzer to activate the MDA mode which uses the LCD like a primary computer monitor 80 characters by 25 rows The LCD is smaller than the standard monitor area therefore just about a quarter of the total screen is displayed 40 characters by 16 rows Quarter 1 on LCD Quarter 2 on LCD to change the actual quarter displayed use the arrows cursor keys on the keypad tofind the cursor press the i button to go back to the normal graphic displaying mode press i again Quarter 3 on LCD Quarter 4 on LCD Definition of displayed LCD quarters in MDA emulation mode 6 3 Key BIOS settings for correct operation The required settings are enclosed in this section rev 1 09 In MDA mode continuous changing of screen may appear To avoid this press left and up arrow on the keypad in any order e MAIN Legacy Diskette A 1 44 1 25 MB Primary Master FlashDisk SDTE XX e ADVANCED PNP OS Installed No Reset Configuration Data No gt l O device configuration Local Bus IDE Adapter Disabled Floppy disk controller Enabled in case of Floppy drive Base I O address Primary Serial Port A Enabled Base 1 0 address 3F8 Interrupt IRQ4 Serial Port B Enabled Base 1 0 address 2F8 Interrupt IRQ3 Parallel Port Enabled Mode EPP Base 1 0 address 378
36. al information about the instrument See menu on the left 31 83 26616 Success ul Press FODH for details self test results fi OF Electr voltage current TEET 49 3 V aba un zo mv A pls Brec 19999 pls 1616 mv 45 mu 965 mBar 224 mbar 2e mbarlbzec 11 8 Y Q2 2 V With the new COMB card the PCPNIF firmware version is empty and the MPU firmware version holds the COMB version number The second and third panel contains tested parameters as follow HGB light LED is on Measuring Electrode voltage current and offset Amplifier Noise test during a 5 second period Amplifier transfer by generating 20000 test pulses incl gain related peak value noise related deviation Atmospheric pressure stands for outer pressure Vacuum reports pump operation vacuum made by the pump in a 10 second period of time Drift represents pressure loss of vacuum measured in a 10 second period of time Power 12V shows the amplifier voltage value Power Batt reports the voltage value of the battery If battery fails system time will stop Core Temp shows CPU temperature of the Control and Measurement Board COMB Overall test result is displayed which can be Successful in case of every test result is OK or Errors if HIGH LOW or ERROR At the end of a result line status message is displayed which means that the actual test result is at the normal range OK higher HIGH lower LOW than the pre d
37. amben Dilutior M3 moves down Needle up rev 1 09 cAbacus Service Manual Microdilutor syncronization Fill up the MIX chamber with 2 5ml of diluent Fill the MIX chamber with 2 5ml of diluent Dilutior M3 moves down Perform wake up process rev 1 09 43 44 Chamber process HGB blank delay Measuring HGB blank RBC chamber draining Diatron MI PLC Dilutor M4 moves down and primes diluent Fill up the MIX chamber with 1 ml of diluent Dilutor M4 moves down and primes diluent Fill up the RBC chamber with 1 ml of diluent WBC chamber draining Lysing rev 1 09 cAbacus Service Manual 45 Dilutor M4 moves down and primes diluent Aperture priming Generating measuring vacuum WBC counting RBC counting HGB measurement RBC chamber draining Pull out 0 2 ml of Lyse WBC chamber draining Fill up the RBC Fill up the RBC chamber with 0 8 ml chamber with 2 5 ml of diluent of diluent rev 1 09 46 Diatron MI PLC MIX WBC Yes No Cleaner Priming WBC chamber Fill up the MIX draining chamber with 2 5 ml of diluent and cleaner Fill up the RBC chamber with diluent Aperture Priming MIX gt WBC High voltage cleaning pulses backflush Dilutor M4 moves down and primes diluent RBC chamber draining WBC chamber Fill up the MIX draining chamber with 2 5 ml of diluent and cleaner Dilutor M4 moves down and
38. cAbacus T Hematology Analyzer oervice Manual Release 1 09 DIATRON Messtechnik Ges m B H A 1141 Wien Ameisgasse 49 51 2 AUSTRIA Tel 431 914 85 00 911 38 48 Fax 914 85 07 15 Web www diatron com DIATRON MI Ltd H 1038 Budapest Papirgy r u 58 59 HUNGARY Tel 361 436 98 00 Fax 361 436 98 00 Web www diatron com E mail support diatron com pistron 2 Diatron MI PLC Revision history pala 11 2006 Team 109 JA Updating tubing schematic May 2007 rev 1 09 TABLE OF CONTENTS L INTRODUCTION ER SE A A ae aa a aaa 7 1 1 NAME AND SERIAL NUMBER o ot 7 1 2 INTENDED USE NE C 7 1 3 INTEGRATED SOFTWARE tattoo 8 FUNCTIONAL DESCRIP TION siii ici areniscas conri 9 2 1 MAIN ELECTRONIC PARTS OF THE ANALY ZER cui aa 9 2 1 1 Counting chambers with electrodes and measuring apertures ss 10 2 12 HOB TAO AG Me ET ES 10 2 1 9 Cell counter Amp Ner DOUA a ase eve tuis ee t St MEM qubd M EU 11 2 1 4 Control and Measurement Board COMB with DIMM PC core eenen 12 21 9 DIME Md uin Pa dd de MM EE 13 2 1 6 Configuration and ID E PROM board IDEPROM serres 13 241 7 Pneumatic and Power Board PPDA etes eat sas ns 14 2 1 6 Opto bourds TOF SIODDOIF INCL OVS EL dt tare ua core t s hs D evite aes 15 2 1 9 ZA a e Pe nt 15 ZAAO SPESIES CUS OF AE N des ch deese mE M RE EMPIRE MEM M EPI MA UE 16 Ziti DudalReagent Sensor BOAO e E ERO EN TRA EE RE RE EIS NE e E EE EE 16 2 1 12 LCD Di
39. data based on Record tag of XML file these structures are locked to HMII SW 2 68m typedef struct DateTime unsigned unsigned unsigned unsigned unsigned DateTime define MaxParams 23 struct TMeasData WORD DateTime WORD char char char DateTime BYTE char float float char WORD BYTE BYTE BYTE BYTE BYTE BYTE BYTE BYTE BYTE float BYTE DWORD float float WORD WORD WORD WORD WORD WORD WORD BYTE char minute 6 0 59 64 hour lt a 0 23 32 day XE E duod 32 gt month 4 LT MD 16 year Tos 0 4095 4096 RecNo 2 TimeStamp Va 4 OperatorID di 2 SampleID 8 8 PatientID 20 20 Name 32 32 BirthDate 4 Sex 12 1 Doctor 16 16 LimitsLow MaxParams 23 4 92 LimitsHigh MaxParams 23 4 92 PatTypeName 20 EA 20 VetMode 2 HistoRBC 256 ES 256 HistoPLT 256 ES 256 HistoWBC 256 LJ 256 PET los fs a PLThi Jel 1 RBClo re dl WBClo LT 1 LYMhi 17 i GRAlo e 1 Param MaxParams 23 KA 92 Flag MaxParams 23 Se 1 23 Warning ih 4 Lyse zy 4 Lyse 2 ES 4 Options EN 2 PrVMinW 17 2 PrVMaxW iy 2 PrVMinR Ly 2 PrVMaxR EJ 2 PrVMinW2 if 2 PrVMaxW2 2 Age A 1 ClogReport 29 Ly 29 11 Total 1240 rev 1 09 bytes bytes bytes bytes bytes bytes bytes bytes bytes bytes bytes bytes bytes bytes bytes byte byte byte byte byte bytes bytes bytes bytes bytes byt
40. ded as Sex 0 Not specified Sex 1 Male Sex 2 Female Note 5 Float numbers are stored on 4 bytes according to the IEEE 745 standard Note 6 Vetmode stores the internal database identifier for the record It is of no importance here Note 7 Histogram representation Each histogram is stored in an array of 256 bytes Each byte represents the histogram value at the given point as a value from 0 to 255 PLTlo lowest PLT volume Actual volume PLTlo x 50 fl 256 PLThi highest PLT volume Actual volume PLThi x 50 fl 256 RBClo lowest RBC volume Actual volume RBClo x 200 fl 256 WBClo lowest WBC volume Actual volume WBClo x 400 fl 256 L YMhi highest LYM volume Actual volume LYMhi x 400 fl 256 GRAlo lowest GRA volume Actual volume GRAlo x 400 fl 256 rev 1 09 94 Diatron MI PLC Full scale and resolution of histograms PLT 50 fl 1 fl x 50 256 RBC 200 fl 1 fl x 200 256 WBC 400 fl 1 fl x 400 256 Note 8 Flags for individual parameters See page 4 remark 4 Note 9 Warning flags see page 3 remark 1 Note 10 Options flag Options field interpretation Option mask Displayed Meaning 0x0001 PREDIL sample was run in pre diluted mode 0x0002 RBCEXT sample was run in RBC extended mode not used in HMII code 0x0004 WBCONLY sample was run in WBC only mode RBC related values filled with 0 0 Note 11 Probe voltages max and min values for separate measurements
41. drained WBC draining MIX 5WBC WBC draining During the last WBC chamber draining the MIX is filled with 5 0 ml diluent Then this volume is moved into the WBC chamber rev 1 09 en lt e Ji 4 2 3 Measurement cycle Before the start of the measurement cycle the instrument is in ready state status LED is green In this state the sampling needle is in the sampling position out and down The Mix chamber is empty the WBC chamber contains 5 0 ml of diluent the wake up process or previous measurement put it into the chamber and the blank HGB measured with this liquid used The RBC chamber contains the last rinsing amount of diluent of the previous measurement cycle or the standby volume The following two flowcharts introduce the entire measurement cycle One of these flowcharts describes the fluidic transfers related the needle the other one the chamber related activities The flowcharts has synchronization points where one of them waiting W for the others signal S Needle process Dilutor M3 moves down Sampling primes diluent Needle washnig needle positioning over the MIX chamber Dilutor M3 moves down primes diluent Needle moves down into the MIX chamber rev 1 09 42 Diatron MI PLC First dilution MIX chamber Dilutior M3 moves down RBC sampling Needle short washing Dilutior M3 Needle positioning moves down over the RBC chamber Second dilution RBC ch
42. e result for RBC Apply user calibration factors function is used to combine user and service calibration factors The software will multiply the existing factors and move them to the Service level to set user factors to 1 00 5 2 6 Settings iB PrieumaticszHeasurement options 2 Operating options 3 Edit Service Code 5 2 6 1 Pheumatic Measurement options Service Person can set the parameters of the automatic functions in this dialog menu Standby time after how many minutes the Standby time 181 68 minis instrument goes to standby mode default 15 Reblank time 8 12 hours min Rinse time B 3 hours Autoclean cycle 9 54 Reblank time after how many minutes the Disable 3 part diff instrument makes a blank measurement if it was in standby mode default 1 hour If it is set to 0 then no ReBlank is performed Disable markers Rinse time If this time is set then the instrument will make an automatic extended washing procedure with more diluent of the chamber s to get wash dirt out of the chambers dust If it is set to O then no Rinse is performed Autoclean cycle after how many measurements the instrument makes an autocleaning Disable 3 part diff for using quick lyse without 3 part differential the errors and the bad 3 part parameters can be excluded from the results Disable markers setting this option to Yes will omit the vertical markers from printed reports rev 1 09 Stretch hi
43. e simultaneously 0 8 ml of lysing reagent is added through V9 On while the Lyse syringe moves upwards This process makes the 1 192 dilution rate and good mixing with lyse M Stepper Motor iN 42 3 way Valve 2 way Valve s 1 3 Off XF Closed Off DILUENT LYSE CLEANER WASTE Li F e WT 232 0n Open On rev 1 09 AAA 4 1 6 Counting process The regulated vacuum it is generated by the pump in the puffer reservoir aspirates the diluted sample WBC and RBC from the chamber through V2 On valve The instrument counts the cells for 5 seconds in both counting phases at first WBC and after it the RBC Micro de us FR V12 ue X vo Hor lt gt V4 Stepper Motor IN Az 3 way Valve 2 way Valve a 1 3 Off XF Closed Off y s DILUENT LYSE CLEANER WASTE LDX Liquid Detector 2 3 On Open On rev 1 09 36 Diatron MI PLC 4 1 7 Aperture priming process In this process the aperture branch and backsides of the apertures are filled with diluent and After generating vacuum in the puffer reservoir the pump continues the drainig of the aperture branch V2 and V3 On V1 and V5 Off meanwhile the diluent syringe of M4 dilutor unit pushes diluent through V10 On V6 On and V8 On Stepper Motor 2 3 way Valve 2 way Valve a 1 3 Off Xx Closed Off DILUENT LYSE CLEANER WASTE T LA Ones Of rev 1 09 cAbacus Service Manual 37 4 1 8 WBC chamber draining process The
44. e then the MID and GRA results are OK but the WBC and LYM results can be higher because of the RBCs Too many RBC cut Repeat the measurement Possibly lyse problem from WBC f the WBC measuring time is too high more than 8 sec it could be aperture clogging In that case perform cleaning and repeat the measurement M WBC coincidence is gt The results are out of the linearity range Make a dilution too high Linearity with an external dilutor with a pre defined dilution range Do error not forget to correct the results with the defined factor ll WBC data package Perform cleaning redo measurement aperture clogging EN errors f it is a general problem please call your Service Personnel WBC time error The same action as in case of the D warning flag C WBC clogging Aperture clogging The same action as in case of the D warning flag Table 9 Summary of warning flags related to WBC HGB rev 1 09 cAbacus Service Manual 81 Warning flags in lowercase refer to RBC or PLT problems Flag Meaning Recommended user action PLT blank is high Repeat the blank measurement and accept it or no PLT blank gt Diluent problem Replace diluent open a new tank RBC blank is high Repeat the blank measurement and accept it or no RBC blank Diluent problem Replace diluent open a new tank RBC PLT limit gt The RBC PLT valley is too high It is rather a diagnostic warning flag If the
45. eel to home position i e home hole d Screw B must be in home sensor and LED corresponding to Screw A home opto sensor goes on Fasten A screws Opto wheel End opto End hole Home opto e Home hole Check the end position as well move the needle down Adjustment is successful if end LED goes on before moving part reaches end of mechanical range Once this adjustment is necessary never miss sampling needle setting described in the next section rev 1 09 28 Diatron MI PLC 3 1 2 Sampling needle setting This adjustment sets the sampling needle to the operational position In Service menu in Miscellaneous submenu of AJ AJvet and in Service menu of AJB select Needle setting The software moves the needle back and up and turns on horizontal and vertical motors AJ AJvet to keep needle in place AJB holds only the vertical motor during needle setting Mis Sampling needle Bottom plane of washing head Teflon stuffing ring Tubes to from the washing head Check the setting of the needle If end of the needle is at the bottom of the washing head needle is set correctly If not open screws B see above and adjust the needle to the bottom of the washing head Fasten B screws Set the end of the tip to the washing head s bottom plane while the carriage is held by motors Needle setting menu Fix the B screws Be careful with the bent upper end o
46. ent was unsuccessful The reblank time has expired rev 1 09 Hemove the reagent tubing and leave waste connected After pressing the analyzer drains itself Connect cleaning tube kit with at least 100 ml distilled water and press V After this the analyzer will be rinsed with the distilled water Hemove the reagent tubing and leave waste connected After pressing the analyzer drains itself Power off the instrument Try cleaning perform blank measurement If the problem still persists use the hard cleaning function Check and empty the waste container Press w If you press you will exit from measurement If you press the blank measurement will start If you press you will exit from measurement If you press the blank measurement will start 7 2 2 Database relating warnings u data record s is There are u data Press L selected records present in the database which match the selection No such type of data There are no u Press and check the data records present selection in the database which match the selection Connect an USB storage This message Connect storage media into the device with data appears at View USB port and press L_w external function data record s will be The analyzer will Insert USB storage media and saved on an USB save9tu number of press v storage device daa recorde Insert a storage device into the USB port If you press the n
47. es bytes bytes bytes bytes bytes bytes byte bytes bytes 5 bits 5 5 DIES 4 pits 12 bits 6 bits Sr wer wr 92 Diatron MI PLC Data map of TmeasData inside an F package Field name explanation note SO be RecNo Internal Record ID 1 2 WORD TimeStamp Timestamp of sample 2 4 DateTime OperatorlD Operator ID Important in multiuser mode only 1 2 WORD SamplelD Sample ID 3 8 char PatientlD Patient ID 3 20 char Name Patient name 3 32 char BirthDate Birth date of patient 2 4 DateTime Sex Gender of patient 4 1 BYTE Doctor Doctor s name 3 16 char LimitsLow Array of Low limits for parameters 23 values 5 23 x 4 bytes float array LimitsHigh Array of High limits for parameters 23 values 5 23 x 4 bytes float array PatTypeName Species name 3 20 char VetMode Internal ID of species 6 2 WORD HistoRBC Array of RBC histogram 256 values 7 256 BYTE HistoPLT Array of PLT histogram 256 values 7 256 BYTE HistoWBC Array of WBC histogram 256 values 7 256 BYTE PLTIO Lower threshold of PLT graph 7 1 BYTE PLThi Upper threshold of PLT graph 7 1 BYTE RBClo Lower threshold of RBC graph 7 1 BYTE WBClo Lower threshold of WBC graph 7 1 BYTE LYMhi Upper threshold of LYMs on WBC graph 7 1 BYTE GRAlo Lower threshold of GRAs on WBC graph 7 1 BYTE Param Array of measured values 23 elements 5 23x4bytes float array Flag Array of flags of measured values 23 elements 8 23 x 1 bytes BYTE array Warning Binary representation of measurement fla
48. etermined limits or the result is an error ERROR rev 1 09 50 Diatron MI PLC 5 1 2 Normal range of Self Test parameters Parameter Unit Lower bound Upper bound wee dark com 0 ww via oise mv 0 o Nosts pisse 0 5 oW manoseo 0 1 cower 76 id 5 1 3 Troubleshooting Guide for Self test Parameter Mark Possible reason Remedy HGB dark HIGH Instrument door open Close instrument door m HGB head not connected or RENE HGB light HGB LED out of order nee HGB LED too bright LED resistor on amplifier board LOW Fault on High Voltage Check measuring voltage 50V on High voltage and Amplifier boards LOW Check current generator and test Amplifier test connected to main board coming from the Amplifier HIGH Instrument not grounded Check mains ground lead Peak of pulses LOW Check current generator and test or HIGH Fault on Amplifier board Jenerator FET on Amplifier board HIGH Instrument not grounded Check mains ground lead HIGH Instrument not grounded Check mains ground lead LOW Pressure sensor cable or Check pressure sensor cable Outer pressure or HIGH connector problem connections and controller board O pressure connector Peristaltic pump failure Check peristaltic pump HIGH Leakage in pneumatics Check tubing in pneumatics rev 1 09 cAbacus Service Manual 57 5 2 Service Menu 5 2 1 Entering to Service Menu There
49. ew will be lost for accepting values will be accepted and all of Are you sure new QC target the measurements on the values selected QC level will be deleted If you press old values remain 7 2 4 Calibration relating warnings Some or al of the During automatic Check the target values and the calibration factors are out calibration at least control blood and repeat the one of the factors are calibration If the problem still out of the range 0 8 persists contact the Service or 1 2 Personnel of range rev 1 09 70 Diatron MI PLC 7 2 5 Hardware relating warnings 5400 12V too low The 12V is not Contact the Service Personnel sufficient the measurements are unreliable 5401 12V too low The 12V is not Contact the Service Personnel sufficient the measurements are unreliable Battery voltage too low The battery has been Check Date and Time settings discharged This problem has no effect on measurement Contact Service Personnel 7 2 6 Other warnings Insert software update Software upgrade After pressing the system media will restart The system will restart 5904 This function will delete Delete If you press all records will all selected records be deleted If you press it will be not deleted 5906 Now it is safe to turn the Shut down process Power off the analyzer instrument off has been finished Now it is time to wipe the Maintenance day You have
50. f the priming After the priming the remaining cleaner between the cleaner detector and V6 approx 1 ml can be dosed into the mix chamber M Stepper Motor iN 142 3 way Valve 2 way Valve 1 3 Off XF Closed Off a DILUENT LYSE CLEANER WASTE EET rs d ain Open On rev 1 09 40 Diatron MI PLC 4 2 Main functions of fluidic system 4 2 1 Initialization Fluidic initialization process performs the following steps e Positioning all mechanical components stepper motors by scanning moving range with end switches Checking the atmospheric pressure Checking of pump and pressure sensor by generating measuring vacuum Priming of reagents and calibrating reagent sensors Fill the tubing with the reagents Cleaning of tubing amp measuring chamber Cleaning of aperture with high pressure back flush cleaner reagent amp high voltage burning 4 2 2 Wake up If the instrument is in standby state the wake up process is taken place in order to make the instrument ready to measure During this process the needle is go out and down to the sampling position and the M1 and M2 stepper motor hold it until the start button pressed or the instrument go into standby While the needle is moving the microdilutor M5 is aspirate some air into the needle this bubble is going to separate the dilent and the blood After this procedure the status LED is turned blue and measurement can be started Simultaneously the MIX and the WBC chamber is
51. f the sampling needle because if badly aligned during movement it can hit other mechanical components causing mechanical jam and therefore damages or error 3 2 Hardware settings 3 2 1 Amplifier offset setting Amplifier offset should be between 5mV Run self test to determine whether offset is within this range If it is out of range it should be re set by the following way Lys x 1 Locate the opening for offset setting potentiometer on the measuring block see enclosed picture In Service menu select Offset adjustment menu Adjust the potentiometer to reach O mV Opening for offset adjustment on measuring block rev 1 09 cAbacus Service Manual 29 4 OPERATION OF THE FLUIDIC SYSTEM This section describes the main steps of Abacus fluidic functions The instrument s Fluidic ochematics are shown in section 2 2 of this manual The following figures show total measurement flow diagram and detailed descriptions of basic processes for understanding the fluidic system work The following steps are introduced in this section 1 Basic processes 1 1 Sampling process 1 2 Needle washing process 1 3 Diluting process 1 4 Fluid transfer from MIX chamber to WBC chamber 1 5 Lysing process 1 6 Counting process 1 7 Aperture priming process 1 8 WBC chamber draining process 1 9 RBC chamber draining process 1 10 Cleaner priming process 2 Main functions of the fluidic system 2 1 Initialization 2 2 Wake up 2 3
52. flows into motors valves or pump rev 1 09 cAbacus Service Manual 15 The green one has 3 states e dark after initialization phase error state e blinking communication in progress normal state e on just lighting OK normal state 2 1 8 Opto boards for stepper motors There are five stepper motors in the system Horizontal and Vertical motors which make the movements of the sampling needle the main Dilutor motors 2 which move the syringes and the micro Dilutor motor which drives the sampling phase The stepper motor opto boards make the connections between the motor driver ICs and motors and have opto switches for the motor s home and end positions The actual status of the stepper motor s optos is indicated by two LEDs on each stepper motor opto boards Dilutor and Micro dilutor have its own separated opto board located directly in the units Horizontal and Vertical motors have a common Opto board called XYopto Board Connections for Hoirizontal amp Vertical motors eeece eg eee 6 06000044400 Opto switches amp LEDs for Horizontal motor Opto switches amp LEDs for Vertical motor The other side of the board contains a ribbon cable connection to the COMB 2 1 9 Valve boards There are two kinds of valve boards Valve board 0 5 and Valve board 6 12 The valve boards are connected to controller and driver chips are located on the PPB lt ___ Valves t Valve Board Connection to
53. flush The RBC chamber rinsed with 5 0 ml of diluent and the remaining cleaner is pushed out with diluent from the aperture branch and back side off the apertures At least the volumes of the standby state are dosed into the chambers 4 2 6 Hard cleaning Before the hard cleaning a wakeup procedure is executed After pressing the start button the M3 dilutor aspirates 0 2 ml from the hard cleaning solution Half of this amount is dosed into the MIX chamber diluted with 5 0ml diluent coming from the other dilutor unit M4 through V10 V8 V6 and the remaining cleaning solution is pushed into the RBC chamber with 5 0 ml diluent see diluting process Then the M4 dilutor unit pulls and pushes the diluted cleaner solution via the aperture combined with high voltage burns After this the chambers are drained and rinsed with diluent 4 2 7 Shutdown The fluidic shutdown performs the following steps e Drains chambers e Perform a cleaning cycle e Priming chamber with diluent to avoid drying out of aperture MIX 2 0 ml RBC 5 0 ml WBOC 5 0 ml e Sampling needle is positioned above MIX chamber needle up e All of the syringes are positioned down rev 1 09 5 CHECKING THE PROPER OPERATION There is a built in Self test and Service menu in the analyzer 5 1 Self test 5 1 1 Self test Screens Self test can be used to check the operation of 17 05 2006 Abacus 150065 SW ABP LB Fiz the instrument The first panel shows gener
54. gs 9 8 DWORD Lyse Volume of lyse reagent used for sample ml 5 4 float Lyse 2 Volume of lyse reagent used for sample ml 5 4 float Options Options set for sample 10 2 WORD PrVMinW Probe voltage min for WBC 11 2 WORD PrVMaxW Probe voltage max for WBC 11 2 WORD PrVMinR Probe voltage min for RBC 11 2 WORD PrVMaxR Probe voltage max for RBC 11 2 WORD PrVMinW2 Probe voltage min for WBC2 11 2 WORD PrVMaxW2 Probe voltage max for WBC2 11 2 WORD Age Age of patient as filled in PatlD dialog 12 1 BYTE ClogReport Clogging information of sample 3 20 char rev 1 09 Note 1 WORD data format is stored back to front E g Decimal value 13553 Hexadecimal value 34 F1 Stored as F134 Note 2 DateTime type is a packed binary format It contains YEAR MONTH DAY hour minute in the following format YYYYYYYYYYYY MMMM DDDDD hhhhh mmmmmm e g 11 27 2005 13 37 is stored as 2005 in binary on 12 bits is 0111 1101 0101 11 in binary on 4 bits is 1011 27 in binary on 4 bits is 1 1011 13 in binary on 5 bits is 0 1101 37 in binary on 6 bits is 10 0101 Concatenating these we get 0111 1101 0101 1011 1101 1011 0110 0101 In hexadecimal these become 7D 5B DB 65 and gets stored back to front It is stored as 65 DB 5B 7D Note 3 Character strings are stored in character arrays space not used within a string is always filled with O s otring to store George Gets stored on 20 chracters as G e or g e 00000000000000 Note 4 Sex is deco
55. he H amp V moving unit and it makes the sample aspirations Correct setting of sampling needle is necessary and very important see Chapter Adjustments 2 2 2 Washing head Washing head is located at the bottom of the H amp V moving unit and it is for cleaning the outer surface of the sampling needle This washing process is made with diluent reagent and the fluid is drained by the pump The arrows on the picture show the direction of diluent flow during sampling needle washing 22 T Clean diluent Pump to waste gt Clean or replace washing head yearly or after 10 000 measurements rev 1 09 cAbacus Service Manual 24 2 2 3 H amp V moving unit This unit contains slides to move the sample sampling needle in Horizontal and Vertical directions two stepper motors XYR opto board opto wheel washing head and the sampling needle It moves the needle to the desired position from sampling position to washing head and to the measuring chamber Sampling needle holder Sampling needle XYR opto board Vertical opto wheel Vertical motor Horizontal motor Both stepper motors have optical end switch sensors for detecting these positions These are required for correct initialization and error detection All sensors have status LEDs to show actual conditions The Vertical motor works with a special opto wheel for detecting home amp end positions See the Adjustment section of t
56. he rear panel 3 screws on the upper side and 2 screws on the vertical side After this pull the cover backwards a few centimeters to release sliding locks and then you can turn it upwards to take it off completely Care for internal printer flat cable disconnect it from the printer connector In this way you will be able to reach the electronics 6 2 MDA Monochrome Display Adapter emulation mode MDA emulation mode was developed to help the manufacturer and Service Personnel in the checking phase of the instruments This mode is available during the total operation but especially useful for checking the BIOS setup and OS functions This mode should be handled the same as the service menu the user does not have to know these options At power on the controller PIC display module displays the greeting screen After 6 seconds it starts to scan the keypad and behaves like an MDA adapter to the PC although the displayed screen is still holding the greeting screen If you switch to MDA emulation mode the system boot events can be displayed and the CMOS SETUP program can be started if necessary If the main program will not start in 90 seconds the PIC will automatically switch to MDA displaying mode to give information about the reason of hang up This can be some CMOS failure if the motherboard settings are lost for some reason and the system is waiting for user confirmation F1 on external keyboard or running setup both require extern
57. his manual to place this wheel to the proper position Greasing of the horizontal vertical guiding rods should be done regularly using Photolube A598 a PTFE based thin lubricant It is recommended to check and repeat greasing of guiding rods every year or after 10000 measurements rev 1 09 22 Diatron MI PLC 2 2 4 Main Dilutor There are two stepper motors a common motor opto board four syringes and piston rods with gear transmission Maintenance should be provided to the piston tips by applying neutral silicon grease to the cogged end of the Macro and Lyse pistons between the syringe and the tip itself This will ensure optimum sealing and longer lifetime of piston tips Greasing of the cogged transmission parts cogwheel and cogged bar should be done regularly using machine grease It is recommended to check and repeat greasing of piston tips and transmission gear every year or after 10000 measurements Lyse syringe and Diluent syringes piston pic Control LEDs rev 1 09 AA A 2 2 5 Micro Dilutor Micro dilutor is taking the precise sample 25 or 50yl into the sampling needle It includes a stepper motor a motor opto board and the micro syringe Connection to Valve Connection to sampling needle Connection to PPB Motor opto board Flexible clutch Stepper motor 2 2 6 Puffer reservoir The glass puffer reservoir is directly connected to the pressure sensor During measureme
58. in the FPGA circuit micro controller This counter counts up while the LED is on and counts down while the LED is off the LED and the counter directions are switched with a 100 Hz signal This method provides real time backlight correction which makes the HGB measurement more precise in changing backlight environment situation as well There are two kinds of HGB measurements e Sample measurement before RBC counting e Diluent measurement in WBC washing phase The HGB result is calculated from these measurements by HGB log CNT gituent light CNT sample light In spite of the fact that Abacus is less sensitive to incident light changes it is recommended to keep side door closed during measurements 2 1 3 Cell counter Amplifier Board Amplifier board includes its own voltage regulator the connection interfaces to HGB head to high voltage board and to COMB card In this board there is the current generator circuit which works from 50 V measuring voltage generated by High Voltage Board and the probe voltage DC is amplified with a voltage follower output ELV Nominal measuring current is 870 pA Amplifier board includes two input connectors for the chambers measuring electrodes There are two reed relays on the input side IC10 can select between the two channels RBC WBC with RSW signal IC11 connects high voltage to the selected probe with HSW signal Test circuit makes possible to generate test pulses with TEST a
59. k disappears In this case you have to identify the possible noise source switch mode power supplies computer monitors since they are not shielded centrifuges due to high switching noise of rotor contacts etc the power of the electromagnetic source because if high power is present maybe relocation does not solve your problems sometimes the electric power supply makes the coupling so UPS solves the problem Another source of coupling in external noise can be the reagent tanks and tubes Especially radio transmitters can cause problems of radiating so that even the reagents diluent guides in the noise A metal pack for the diluent tank then a good earth grounding of this metal box allows this coupling to disappear forever 7 3 4 Internal noise sources The most annoying but real cause is some sort of internal noise The reason for this phenomenon is that inside electrode hot point of the measuring circuit must be well insulated from surrounding electronics otherwise inside noise sources can take their effect 7 3 4 1 Bad chamber insulation bad shielding of the chamber floating shield couples signals to the chamber and does not prevent against them Check grounding of shield remove it and clean the surface between the shield and the metal base bad reference electrode connection floating ground reference Repair is required bad sealing of aperture Replacement of measuring tube is required broken mea
60. kages sent by Abacus are placed between a header and a tail There are 5 main types of them INIT DATA and histogram packages XML descriptor and FULL record The histogram package can be RBC WBC and PLT Format is the same only the type descriptor differs INIT package Abacus sends the general identifiers by this package during initiation of a communication DEVICE lt HT gt VERSION lt HT gt DATE lt HT gt TIME lt HT gt DB VERSION DEVICE device identifier Abacus VERSION version of the software e g 1 3 DATE date in YYYYMMDD format TIME time in HHMMSS format VERSION Database version in Mmm Major minorminor format rev 1 09 84 Diatron MI PLC DATA package This package contains sample and patient data the measured parameters and the markers of histograms One data element consists of two items name and value which are separated by a HT character and closed by lt LF gt SNO lt HT gt 152 lt LF gt internal identifier DATE lt HT gt 19980715 lt LF gt date of measurement YYYYMMDD TIME lt HT gt 114500 lt LF gt time of measurement HHMMSS PID lt HT gt 2 lt LF gt patient identifier 0 9999 NAME lt HT gt JOE SMITH lt LF gt patient name max 32 characters MODE lt HT gt O lt LF gt patient type WRN lt HT gt 0 lt LF gt warning bits 32 bit hexadecimal PM1 lt HT gt 12 lt LF gt PLT lower marker PM2 lt HT gt 204 lt LF gt PLT upper marker RM1 lt HT gt 51 lt LF gt RBC lower marker WM1 lt H
61. le Clean the draining path Do not use alcohol but bleach Replace chamber if necessary liquid remains in the washing inlet at top of the chamber during measurement the conducting liquid remains inside the chamber wash tube making noise to appear The software is not compatible with the mechanics or related valve is bad partly clogged or the tubing is clogged loose lyse path guides in noise during counting if the a liquid in the draining tube is touching lyse reagent in T fitting noise can appear Check the lyse path and the lyse valve as well rev 1 09 74 8 1 8 1 1 8 2 8 2 1 8 2 2 8 2 3 8 2 4 8 2 5 8 2 6 Diatron MI PLC 8 MAINTENANCE Weekly Maintenance by User User should carry out on the first workday before starting up the analyzer Cleaning the washing head User should clean the lower surface of the sampling needle washing head using a soft cloth immersed in warm tap water to remove salt build up Periodic Maintenance by Service The instruments should be checked and maintenance must be carried out in every 6 months or after 10 000 measurement cycles Check Self test and Device statistics Run the built in Self test and check the overall test result Check the device statistics to find common problems Cleaning and Greasing Dilutor Block The dilutor block driving wheels and gear bar should be cleaned from dirt and must be greased with A597 grease between the gear bar
62. measurement or absolutely lost 1106 Pneumatical error Fatal error in If frequently happens this error occurred mechanical check the proper operation of the Device s subsystem motor modules home end opto Vacuum error The measuring vacu Leakage in the pneumatic system um has been check the pump fittings tubings 1105 dramatically pressure sensor puffer reservoir Error code s switches ribbon cables and Status x moving of motors Retry 1107 Check Cleaner container Cleaner reagent Replace the Cleaner reagent container became container with a filled one empty or the clean lIf this error still remains with a fluid sensor is too filled cleaner reagent container sensitive or there are calibrate sensors and check the lot of bubbles in the tubings and the fittings cleaner tubes 1113 Unrecoverable Fatal error in Check the proper operation of the pneumatical error mechanical motor modules home end opto occurred n subsystem Possibly switches ribbon cables and the Device s n mechanical jam motor moving Error code s n Status x n Please turn off the instrument and turn it on again 7 1 3 Measure errors 1200 Fatal Measinit error Fatal electronic error Call service Power off the system 1201 Fatal HGB error HGB channel did not Call service Power off the system give a ready signal rev 1 09 66 Diatron MI PLC 7 1 4 Printing errors 1400 Printer error d Pri
63. ment 3 Clear databases Clear Device Statistics Device statistics 4 Offset adjustment number of measurements aperture clogging and other errors can be cleared S Needle setting Clear Database The whole Database measurement results including histograms can be cleared Offset adjustment You can adjust the offset on the amplifier board Needle setting By entering this menu the needle will go to the position you can adjust it You can Log in as supervisor if the Multi user mode was selected in the User s Settings submenu Number 4 if present shows the availability of this function In supervisor mode you have the ability to change any user passwords and you have full access over user settings Always log out after this supervisor log in 5 2 13 Multi user Rescue Code It the supervisor password has been forgotten there is a rescue code for service purposes to access the supervisor level The Service user data are as follows User ID 0 zero Name Service Password 729456 This forms a capital A on the keypad rev 1 09 58 Diatron MI PLC 6 SERVICE OPERATION 6 1 Opening the instrument On the right side of the instruments there is a side door which allows reaching of the fluidic system and the mechanical parts Other parts of the analyzer electronic parts etc can be reached by opening the top left cover e How to take off the top left cover First unscrew the 5 cover fixing screws located on t
64. nd PLS signals through FETs for checking the proper operation of the amplifier channel Connection to the HVB Reed relays Connection to COMB AMP and DIGIO rev 1 09 12 Diatron MI PLC Amplifier board includes a 3 stage main amplifier channel which gains to the input signal to the 0 5 V range this is the input range of the A D converter which is placed on the COMB card There is an offset potentiometer P1 in the third amplifier stage manufacturer sets the correct offset voltage Adjust the offset voltage only in case it is out of the 5mV range DHON signal from the COMB card switches on the LED in the HGB head via a transistor Q3 but the Photo Detector in the HGB head is working continuously The other side of the amplifier board contains special connectors for the chambers and the HGB head 2 1 4 Control and Measurement Board COMB with DIMM PC core The compact COMB incorporates a single PC and its environmental functions as well as the specific measurement processing functions in one board PS2 COM 1 ad USB B USB USB n TT hn Li ve gt S il ste SR LPR NUTS Speaker Siem ut C MA ey l MAA AA Ml IDEPROM connection HVB connection Display connection lt Floppy connection IDE connection Amplifier connection DIGIO PC system of the COMB board is based on the DIMM PC module which is a credit card size PC with AMD Elan SC520 133 MH
65. nt there is no pump activity so the puffer reservoir maintains measuring vacuum stable The instrument measures atmospheric pressure and adjusts measuring vacuum according to it 2 2 7 Pump Pump generates regulated vacuum and drains the fluidic system It is connected to the PPB and it has its own driver circuit Darlington If the tube of the peristaltic pump becomes worn it can be broken causing Pressure error It is recommended to check the state of the tube and replace it every 2 years or after 20 000 measurements Always replace the peristaltic pump tube to the same PharMed type with the same length For servicing the tube of the pump open the peristaltic pump from its top see picture and remove the tube together with the white plastic side wall see picture J 883ld TU In case of damaged tubes it can be replaced by a new one by opening the two metal locks located at the two ends of the tube see picture rev 1 09 24 Diatron MI PLC 2 3 Assembled Analyzer 2 3 1 Abacus Front Panel Built in thermal mme M printer optional 240x128 dots Graphic LCD Foil keypad Function keys START button Rear panel Power switch 12V DC inlet Warning labels USB ports Serial Port USB slave port PS 2 external Reagent inlets keyboard port rev 1 09 cAbacus Service Manual 25 Construction front Valve block Pressure sensor Electronic block H amp V unit Speaker
66. nter error Check the connections between the instrument and the printer and check the printer setup in the Settings Printer settings sub menu 1401 Printer is out of paper Printer is out of paper Feed the printer with paper Check and error d and there are some the connections between the other printer errors instrument and the printer and check the printer setup in the Settings Printer settings sub menu 1402 Printer is out of paper Printer is out of Feed the printer with paper paper 1403 Printer was not setup The selected printer Modify the printer setup in the correctly type does not match Settings Printer settings sub menu to the printer large over the margin Settings Printer settings sub menu large over the margin Settings Printer settings sub menu small over the margin Settings Printer settings sub menu small over the margin Settings Printer settings sub menu large oettings Printer settings sub menu large Settings Printer settings sub menu Spacing is too large The distance Modify the vertical spacing setup in between two results the Settings Printer settings is too high submenu Error d in printer Printer software error Check the connections between the ES 1410 initialization instrument and the printer and check the printer setup in the Settings Printer settings sub menu Try to make a software upgrade 1411 rev 1 09 7 1 5 Other errors You have to add a
67. of a package identifier and a package type descriptor The tail includes the checksum Typical format lt SOH gt MID CMD lt STX gt MESSAGE lt ETX gt CHKSUM lt EOT gt MID Message ID one capital letter between A and Z CMD Command one capital letter package type descriptor Por INIT D for DATA R for RBC W for WBC P for PLT for XML descriptor e F for full record transmission MESSAGE the message consists of ASCII characters between 32 128 CHKSUM two hexadecimal digits of a one byte checksum which contains the lower byte of the sum of characters between the lt SOH gt and lt ETX gt incl those too 10 2 3 Format of Acknowledge of the Receiver The receiver must acknowledge transmission by the following message lt ACK gt CMD MID CMD the type of the next package to send this makes possible to a request for histograms If no more packages are required SPACE should be sent MID the identifier of the package that acknowledged by this message If receiving of the message was not successful lt NAK gt should be sent and Abacus will repeat the last package The receiver has approx 1 second to reply Otherwise Abacus will repeat the last transmission twice automatically But if still no response after 3 trials Abacus will not start to communicate any more even if there is data to send see General Description 10 2 4 Detailed Description of Packages The pac
68. ole blood sample e WBC LYM MIDA GRAF LYM MID GRA three part WBC differential e HGB RBC HCT MCV RDW MCH MCHC e PLT MPV PCT PDW rev 1 09 8 Diatron MI PLC 1 3 Integrated software The integrated software controls the instrument operations displays stores recalls data and allows the user to perform QC and calibration procedures and modify the user settings The software version number can be read out from the Device Information or from the Self test submenu Software is absolutely Plug and Play it can read out and detect the type and the serial number of the instrument therefore it will run the correct program for the hardware without any user or service help Every Abacus software version is upgradeable using an USB drive by the latest program developed by DIATRON and it can be downloaded from WWW diatron com rev 1 09 Abacus Service Manual 9 2 FUNCTIONAL DESCRIPTION 2 1 Main electronic parts of the analyzer Abacus contains the following electronic parts 1 Counting chambers with electrodes and measuring apertures 2 HGB Measuring Head 3 Cell Counter Amplifier Board behind the chambers 4 CPU Board with DIMM PC and measurement processing unit COMB Board 5 Pneumatic and Power Board PPB with motor controllers valve amp pneumatic controller pump driver and power supply for internal printer 8V and digital circuitry 45V 6 Safe configuration
69. onnector Put the MS DOS boot floppy into the floppy disk drive After turning on go to the BIOS setup press F2 repeatedly on the keyboard and switch to MDA mode by pressing i Change the Boot Device Priority in the BOOT menu The Removable Devices must be the first in the list Select Removable Devices and move it up by pressing on the keyboard Save the actual settings to CMOS the instrument will restart Exit Saving Changes The instrument will boot from the floppy and the software will be terminated owitch to MDA mode by pressing i Do not forget to restart the instrument and set back Boot Priority list after servicing Hard Drive first rev 1 09 7 TROUBLESHOOTING The analyzer checks the operations of several mechanic fluidic and electronic parts during measurement The system shows the type of the error on the LCD display if any kind of malfunction is detected The electronic parts have a very little chance to fail only the connections and cables could disconnect which can cause the malfunction of the electronic system The mechanic and fluidic system have a bit more chance to go wrong because it has moving parts This section allows to know what to do when a troubleshooting message appears on the Screen rev 1 09 64 Diatron MI PLC 7 1 Error codes s filename concerned d error type concerned u error specific string 7 1 1 Software system errors YS program error 1000 program error
70. primes diluent Fill up the RBC chamber with 4 5 ml of diluent rev 1 09 cAbacus Service Manual MIX gt WBC Autoclean Yes NO down and primes High voltage diluent cleaning pulses Aperture Priming Dilutor M4 moves down and primes Dilutor M4 moves diluent 4 2 4 Standby 47 Standby process is executed when the instrument is in ready state and the standby time see Service Settings ellapsed In the beginning of the standby process the needle goes up and is washed and the chambers are drained After this the 3 0 ml diluent is put into MIX via needle and the RBC chamber via V8 and the diluent from the MIX is transferred into the WBC chamber and at last MIX chamber is filled with 1 ml of diluent and the back side of the apertures are primed The diluent in the chambers prevent the apertures and chambers from dirt drying out and salt build up rev 1 09 48 Diatron MI PLC 4 2 5 Cleaning Cleaning function drains all of the chambers and fills the RBC chamber and the MIX chamber with 2 5 ml of diluent After moving content of the MIX chamber into the WBC chamber the aperture branch is primed with cleaner see 1 x The remaining cleaner between the cleaner detector and the V6 valve is pushed into the MIX chamber with diluent After this the WBC chamber is drained and the cleaner with diluent transferred here from the MIX chamber Then the apertures are cleaned with high voltage burn and with back
71. r with 3 ml of diluent 1 0 ml is waiting in the MIX chamber which comes from the Macro syringe through V11 On and Micro dilutor while the M3 Dilutor motor moves upwards This process makes the 1 160 first dilution rate in the MIX chamber i JJ ej nn L T St Mot IN Az 3 way Valve 2 way Valve D di Xm pta e XF Closed Off DILUENT LYSE CLEANER WASTE LDx Liquid Detector 2 3 On Open On The second sampling process aspirates 35 ul of primary dilution and then it is added with 4 0 ml of diluent 1 0 ml 3 0 ml into the RBC chamber This process makes 1 18285 dilution rate in the RBC chamber rev 1 09 or CM 4 1 4 Fluid transfer from MIX chamber to WBC chamber In Abacus during this step the liquid remaining in the mixing chamber flows via the VO On into the WBC chamber because the vacuum which is in the puffer reservoir and maintenated by the pump aspirates the fluid through V5 On and V3 On while V2 and V1 are Off Simultaneously the peristaltic pump supports this procedure via V4 On Micro us LES MIX e De vo Hor EXE qv Pump a gt Stepper Motor iN 142 3 way Valve 2 way Valve a 1 3 Off nn Closed Off DILUENT LYSE CLEANER WASTE Lisci Elli WT 23 0n Open On rev 1 09 34 Diatron MI PLC 4 1 5 Lysing process During this step the primary dilution remaining in the mixing chamber flows into the WBC chamber like a simple MIX WBC transfer but in this cas
72. sampling needle with diluent in the washing head after sampling It is important to clean the outer surface of the sampling needle to avoid inaccurate sampling The Macro syringe doses and the pump drains the diluent from the washing head while the sampling needle moves upwards so that the total length of it is washed and cleaned This process is called total sampling needle washing and it is mainly used after taking primary sample from sample tube Micro 6 ra E RBC MIX e i X X v gt M2 Stepper Motor iN 42 3 way Valve 2 way Valve T Closed Off 1 3 Off DILUENT LYSE CLEANER WASTE iii ME sub Open On Another process which is washing only a smaller part of the sampling needle is the same but the needle does not move in the total length Some procedures perform this kind of sampling needle washing The M3 Macro syringe pushes the diluent through V11 Off V12 On The Pump aspirates the diluent from the washing head through V4 On while the M2 Vertical motor moves the sampling needle up rev 1 09 32 Diatron MI PLC 4 1 3 Diluting processes Before the dilutions the MIX and the RBC chambers are filled up with 1 ml of diluent This method prevents the chamber from dirt and makes the diluting process faster The sampling process has aspirated 25ul of sample or 35ul of primary dilution which is in the sampling needle In the first diluting step the sample is dispensed into the MIX chambe
73. splay Module with High Voltage Board eese enne eene 17 ze A cec mL M EU M M M M a idu ac 18 2 l4 EXCITA Power SUD eet tuto dvds A dva Istuc Na 18 ds MAIN MECHANIC AND FLUIDIC PARTS OF THE ANALYZER oocccccccccccncnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnos 19 B2 ds SEMPRON bed cire A ENT Me de 20 22 2 WARE OA Se X 20 22 HANV MOVU E UNU EN NN 21 2 2 4 NEG DOT UT ON e a E ES I aM EM E ML I DM 22 22 MECO DINO E eee 29 2 2 6 DUCK TOS CPV OITA ene MC UE LM LE DE UC DI a LU 23 2 2 4 POIDS RS RER E 23 2 95 ASSEMBLED ANALYZER einst ERR M Mui ME E i ue 24 2 3 4 POTS A A 24 ADJUSTMENT ota aa e Aaa EN RE ule ER EE PS ET 27 3 1 MECHANICAL SETTINGS NEP MTS 27 3 1 1 OMO We SOINS al dl 27 3 142 SAMPLE CONE CUT PASE e bale Oy asa atm it epus ues Eno a dila ime dd 28 2 52 HARDWARE SETTINGS aan 28 SUN M AMENER OSEO TERRE EU et abate en tunes 28 OPERATION OF THE FLUIDIC SYSTEM osscccioniiocecicnnnnicacinasociccacnnicacicca senora caco aoo ne aaa paea ciencia aeai 29 4 BASIC PROCES SES M 30 4 1 1 SAMPUNE PVOCESS ats ht caen avenant dal min een d uda AGS 30 4 177 Needle WASHING DIOCESS ee AS a Mac e E LUN MI a EO LE 31 4 1 3 Diui PEO CES E itt te Re vault eU MU else 32 4 1 4 Fluid transfer from MIX chamber to WBC chamber eese nnn 33 4 1 5 TEV SUVS DOCS me ta ha
74. splay and the high voltage board LCD has a high voltage backlight lamp high voltage board generates the required voltage There is a special temperature compensation circuit in the display module which makes possible to use the LCD module in wide temperature ranges with the adjusted contrast High Voltage Board HVB generates LCD backlight voltage 300V aperture cleaning voltage 150V and measuring voltage 50V The high voltage board is connected to the system through the amplifier board and the COMB card This unit contains INVC191 inverter which is a high voltage high frequency circuit producing suitable voltage for CCFL cold cathode fluorescent lamp of the LCD The CFSW digital signal from the COMB card controls HVB logical LOW turns inverter on The MVON digital signal from the COMB card switches the measuring voltage 50 V on off by O1 opto switch Warning Be careful with servicing this board in active state because the high voltage 300V at LCD lamp connector can cause damages or electric shock Start key is a micro switch connected to the COMB card through the Display ribbon cable The status LED indicates the actual status of the analyzer and it has two colors red and blue See Users Manual The LED has three pins and the actual color depends on the controlled pins Start key and status LED are controlled by COMB Connection to COMB and amplifier Connection to LCD lamp rev 1 09 18 Diatron MI PL
75. stogram setting this option to Yes Stretch histogram will modify the WBC histogram range from O 300 fl instead of 0 400 fl this is recommended for Veterinary mode Reagent cade Extend Frobevoltages YES Maintenance Dau Wednesday Reagent code This code can be used to Laboratory Header Lines modify the offset of the HGB function Enter a Reagent Pack value between 0 and 30 default is 9 Extended Probe Voltages This option is ON by default It makes the instrument accept the physically highest probe voltages to allow operation at lower operating temperature range Maintenance day You can select from week days According to this setting the instrument will ask the user to do weekly maintenance during shut down Laboratory header lines you can choose how many lines to contain the header of the blood result Reagent pack Using packed reagent in Reagent status menu system displays the reagent pack installation day and the Reagent open stability period User can change reagents only together In case of No reagents can be changed separately the date of expiry is ignored Pheum Meas Settings Waste container capacity it essential to set Reagent open stability days the correct volume of the waste container for proper usage of the software waste full alert code 5006 Set this value two liters less than the total volume of waste container Waste container capacity 6 68 l Diluent Container Capacit
76. suring chamber starts to conduct through the gaps ground path Replacement of chamber is required contaminated draining tube starts to conduct due to protein or lipid build up It is very easy to identify this case After replacing the drain tube of the measuring chamber mainly WBC WBC histogram peak or PLT becomes low soon Normally a good cleaner is required to dissolve lipid or protein build up Sometimes the cleaner is not strong enough to keep this tube clean enough Periodic washing using 1 hand warm bleach solution helps rev 1 09 cAbacus Service Manual 13 7 3 4 2 Bad insulation of electronic signal paths In these cases check for any capacitive coupling of electronic signals to the chamber interference with HGB head high frequency signal is coupled to the chamber HGB head metal parts must be grounded The ground comes externally it must be in place otherwise HGB head does not shield but couples in noise interference with internal high voltage inverter high frequency signal is coupled to the chamber Repair is required avoid near contact of HVB cable to chamber or shielded amplifier cable interference with internal start button polling signal to start button may cause noise Guide start button wires as far from chamber as possible You may try mix them up on the start micro switch if applicable interference with display cable high frequency LCD signal is coupled to the chamber by the ribbon cable
77. t LF gt PO1 lt HT gt 6 6 lt HT gt 0 lt LF gt P02 lt HT gt 4 29 lt HT gt 0 lt LF gt POSCHTO DOZJISHISUSHBO sge PATIENTS 3 2H T gt 0 lt 0F gt 2B22 lt H1 gt 43H 1 gt 0 lt LE gt lt ETX gt 7C lt EOT gt Receiver acknowledges DATA transmission and requests for RBC package lt ACK gt RB Abacus sends RBC package lt SOH gt CR lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt SID lt HT gt 2 lt LF gt PID lt HT gt 26 lt LF gt CHN lt HT gt 256 lt LF gt 9 lt HT gt 16 lt HT gt 26 lt HT gt 1 lt HT gt O lt ETX gt F2 lt EOT gt Receiver acknowledges RBC transmission and requests for WBC package lt ACK gt WC Abacus sends WBC package lt SOH gt DW lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt 19980715 lt LF gt TIME lt HT gt 114500 lt LF gt SID lt HT gt 2 lt LF gt PID lt HT gt 26 lt LF gt CHN lt HT gt 256 lt LF gt 0 lt HT gt 15 lt HT gt 84 lt HT gt 5 lt HT gt 3 lt ETX gt 69 lt EOT gt Receiver acknowledges WBC transmission and closes the link lt ACK gt lt SPACE gt D It is possible to request for PLT histogram but it is not shown in this example rev 1 09 90 Diatron MI PLC 10 2 7 Serial Protocol 2 20 2 23 This version utilizes a bit upgraded header information lt SOH gt BD lt STX gt SNO lt HT gt 152 lt LF gt DATE lt HT gt L9980 71 15 lt LF gt TIME lt AT gt 114500 lt LE gt SID lt HT gt 2 lt LF gt PID lt HT gt 26 lt LF gt NAME lt HT gt JOE SMITH LF MODE HT O LF WRN
78. the system which decreases volume of lyse reagent Check the hardness of tubings at rear panel lyse connection by hand If you feel the Tygon tubing hard compared by other tubing replace them Bleaching of Fluidic System It is recommended to run a bleaching procedure to remove stains from the fluidic system Connect 2 596 hand warm clean bleach solution to all reagent inputs and perform priming on all reagent inputs Leave it in the tubing for not more than 2 3 minutes Remove the bleach prime on air Connect distilled water 100 ml and perform priming all reagents again Connect reagents and run priming again rev 1 09 76 Diatron MI PLC 9 SPARE PARTS coe CONES APX104 Front panel Abacus excl keyboard rev 1 09 cAbacus Service Manual MIA J5P900 Microdilutor unit complete rev 1 09 N 7 78 Diatron MI PLC com NA A552 Colour lock nut inside A553 Colour coding ring outside A564 Fixing for pump pair A565 Connector for pump pair J7502 84P ribbon cable XY block 7503 hoP ribbon cable printer AP70 Ground cable 15 cm AP702 Ground cable 25 cm AP708 Ground cable 62 cm AP750 OP ribbon cable reagent sensor AP7502 20 14P ribbon cable LCD AP7503 POP ribbon cable VALVE AP7504 0P ribbon cable HVB board rev 1 09 cAbacus Service Manual 34P ribbon cable amplifier board AP7505 AP7506 AP7507 AP7508 AP7509 AP132 A
79. tleast Multi user problem Follow the instruction or change one user to use multi to single user mode in the User user mode settings submenu s sensor calibration Reagent container Replace the reagent container failed became empty or with a filled one The system turned off the there are lot of If this is still a problem with a S sensor bubbles in reagent filled reagent container turn on tubes the sensor and try recalibrating and check the tubings and the fittings rev 1 09 68 7 2 Warning messages 7 2 1 Pneumatic warnings Diatron MI PLC Remove reagent tubing at rear reagent inputs Diluent Lyse and Cleaner 9001 9002 9003 9004 9005 9006 9008 9009 Connect min 100 ml distilled water to reagent inputs using cleaning tube kit Remove cleaning tube kit Keep reagent inputs free You can power off the system Apertures are partially clogged Try cleaning Please waste container empty There are no accepted blank values Measure blank The blank expired results are Measure blank First message of preparing for shipment Second message of preparing for shipment Third message of preparing for shipment The preparing for shipment function is finished During the measurement one or both of the apertures were clogged C for WBC c for RBC aperture The calculated waste volume has reached its maximum level The last blank measurem
80. tries is not listed in the XML file You will find them below define PAR WBC 0 define PAR RBC 1 define PAR HGB 2 always transmitted in g l unit define PAR HCT 3 define PAR MCV 4 define PAR MCH 5 7 7 always transmitted in g l unit define PAR MCHC 6 define PAR PLT 7 define PAR PCT 8 define PAR MPV 9 define PAR PDWsd 10 define PAR PDWcv 11 note that it matches with PAR PDW define PAR PDW 11 note that it matches with PAR PDWcv define PAR RDWsd 12 define PAR RDWcv 13 note that it matches with PAR RDW define PAR RDW 13 note that it matches with PAR RDWcv define PAR LYM 14 define PAR MON 15 define PAR GRA 16 define PAR LYMp 17 define PAR MONp 18 define PAR GRAp 19 define PAR RBCt 20 define PAR WBCt 21 define PAR WBCt2 22 Use the above identifiers to interpret entries in the array PARAM and FLAG interpretation should happen accordingly This package starts with the X package identifier contains 2 size descriptor bytes SIZE1 and SIZE2 and the XML file in ASCII format Use this as a descriptor of the structure sent in F package lt SOH gt lt MESSAGE_ID gt X lt STX gt lt SIZE1 gt lt SIZE2 gt lt PAYLOAD XML file in ASCII format SIZE1 256 SIZE2 BYTES gt lt ETX gt lt CHKSUM1 gt lt CHKSUM2 gt lt EOT gt FULL record package This package starts with the F package identifier contains 2 size descriptor bytes SIZE1 and SIZE2 and a record in binary format
81. tron Embedded Modules GmbH 2 25 26m 5 EREEE mam i Flash BIOS IT ERE Dugun Hard Disk FlashDisk uiii M N FlashDisk controller 32 Mbytes RAM LUTTEFEETETTTFETETTTTTI UHR HEEL RNCS 4 Clock generator On board SMPS Super I O AMD Elan SC520 CPU Edge connector 2 1 6 Configuration and ID E PROM board IDEPROM This board is the interconnection between COMB and PPB cards Pneumatic l C bus power lines and internal printer signals are connected through this card The board also contains a 24FC256 serial EPROM which stores the FPGA s configuration data and identity information of the instrument Serial Number OEM model etc EPROM 00000000000000 Keeping the hardware identity information write protected IDEPROM allows running the correct software rev 1 09 14 Diatron MI PLC 2 1 7 Pneumatic and Power Board PPB PPB card contains the main power regulator circuits valve and motor driver circuits and other connections for the fluidic and pneumatic system s parts PUMP MDIL X Y SR Peristaltic pump Micro dilutor Horizontal Vertical amp connection connection Sample rotor connection PRESSURE ud Pressure sensor E uasa N L am No Q connection NC z aeiee REAGENT SENSOR A Reagent sensor CA MED connection d DIATRON 1 Hilos Ml LEC ESOSESOCOC OL 2 e bbiliiiiiiicR gt lI LM IA a 4 elp L p 2 y
82. uration values for this menu lt F10 gt Save and Exit lt Enter gt Execute command or select submenu lt Alt R gt Refresh screen To select an item simply use the arrow key to move the cursor to the field you want Then use the plus and minus keys to select a value for that field The Save Value commands in the Exit Menu save the values currently displayed in all the menus To display a sub menu use the arrow keys to move the cursor to the sub menu you want Then press lt Enter gt pointer 4 marks all sub menus The Field Help Window The help window on the right side of each menu displays the help text for the currently selected field It is updates as the cursor is moved to each field The General Help Window Pressing lt F1 gt or lt Alt F1 gt on any menu brings up the General Help Window that describes the legend keys and their alternates Press lt Esc gt to exit the General Help Window The Main Menu You can make the following selections on the Main Menu itself Use the sub menus for other selections rev 1 09 62 Diatron MI PLC 6 6 DOS functions on the instrument To run the computer part of the instrument like a normal PC the Service Personnel should have a floppy drive with cables see chapter 9 5 and an MS DOS 6 22 operating system boot floppy disk Take off upper cover of instrument Connect an external keyboard Connect the floppy drive to PPB board Floppy CD power connector COMB board FLOPPY c
83. ut if still no response the other side will be supposed to be not ready to receive data and thus Abacus will not try to communicate any more From this state it can wake up by receiving an lt ENQ gt ASCII code 5 character from the other side and Abacus will immediately respond with an lt ACK gt ASCII code 6 From this point Abacus will send data if it is ready for transmission The communication is based on packages There are 7 package types INIT package Device identification software version current date and time DATA package Sample and patient information measured parameters and markers RBC package Sample information RBC histogram WBC package Sample information WBC histogram PLT package Sample information PLT histogram XML file with FULL record structure information FULL record transmission The communication sequence is always started with an INIT package If the link is successful Abacus will send DATA package and the receiver can request RBC WBC and PLT packages at acknowledge If serial communication protocol is set to version 3 0 Abacus will send XML and FULL packages Special characters used in the communication lt STX gt lt ETX gt lt EOT gt lt ENQ gt lt ACK gt HT a rev 1 09 10 2 2 Format of Packages Sent The packages sent by Abacus are always between a lt SOH gt and lt EOT gt character and they consist of header data and tail The header consists
84. v d vedi E A ei ode ten 34 4 1 6 BT RA o a nn nsc dE RR 35 4 1 7 APTE PTIPUIN E DIOC SS ricas 36 4 1 8 WBC chamber draining aaa 37 4 1 9 RBC chamber aranin o DFOCOSS s ets on E E o AA EE M Wade teda NUS 3 4 1 10 Cleaner DEUIn DIOCOSV x itt attt vou A tt t Nm ee 39 q 2 MAIN FUNCTIONS OF FEUIDIC SYSTEM nt ad 40 4 2 1 TUT ON allo ete nivis A A Sn e M MR 40 4 2 2 Wat UP nM Sd AC Si MI 40 4 2 3 AAA og abet ear ner etes cope tec ua 41 4 2 4 A ec de ea pada E sO meg sae A ES 47 4 2 9 IT e RR TRO HORN 46 4 2 6 IE ERA ETOO 46 4 2 7 CON o Po e O E 48 4 Diatron MI PLC 5 CHECKING THE PROPER OPERATION isa sen sso bre anti papa coneccion ti faeettedetenetedese 49 5 1 SELE TES o oia 49 5 I I SCIT TEST OCC St dite d ette bes vet ies 49 241 2 Normal range Of Self Test Paramet eiS tin tt t Oe dd 50 5 1 3 Troubleshooting Guide Jor Self te unit Eve T te ea dT 50 342 SERVICE NENU en eheu et en te 51 5 2 1 ENTEROS EICO MEU das 51 5 2 2 MAN SEV EM dida 51 52 3 EE SAV Ce CAN dci 51 5 2 4 DEVICE O TOTO AAA ANA SAS 51 3 259 SV CE CANDIA TON A Rd 92 5 2 6 SY AL T RR TT TP TRE 52 7 Service Testi o Menara den e OU oe Ata ge 55 5 2 6 VAWE EST MORE detis A AR en ner A eA bett amo at dofus 55 5 2 9 MOTOR TES CAMERA ias 55 W240 IIISpPlayv and Keyboard Tesis tU n EUIS de veste UE cM estu tU e up EU USE 56 5 2 11 SEF OSS VOC PERPE 56 22125 Miscellaneous Seano S sheet de ne M A cidre 57 Od Mulli ser RESCUE C OUO AA
85. y ml Lyse Container Capacity ml Cleaner Container Capacity mls Rinse Container Capacity ml Lyse container capacity Enter here volume of lyse container Cleaner container capacity Enter here volume of cleaner container Rinse container capacity For future development Enter here 0 It is possible but not recommended ignoring the reagent volume check entering 0 as volume of containers Pneum MeasSettings Clogging detections bubble volumes for Clogging detection development do not touch them Bubble volume before Mls sample Bubble volume after Mls sample Bubble volume before REC sample Bubble volume after REC sample rev 1 09 54 Diatron MI PLC 5 2 6 2 Operation Settings LCD light off delay LCD backlight switches LCD light off delay mini off after 4 hours by pressing key switches back Still the light is off the LED is flashing yellow Disable multiuser made Instrument startup modes Database Database Capacitu records 1815 Disable multi user mode by this setting the Hative keyboard YES multi user mode can be disabled Calendar Mode Gregorian Instrument startup procedure You can select to start the instrument with Database screen without any pneumatic initialization or with Measurement screen with pneumatic initialization Database capacity You can choose here the capacity of database at installation Changing later all measured data will be lost Using larger database the
86. z micro controller DIMM PC itself contains 16 or 32Mbyte RAM and same size of FlashDisk that acts like a hard disk DIMM PC module is easily replaceable as it has an open socket it has also a screw for safe fixing COMB card contains single ICs and some drivers protection circuits for the interfaces such as COM1 PS2 USB IDE and Speaker Measurement processing is based on a FPGA circuit After power on the FPGA holds the DIMM PC in wait state with IOCHRDY signal until the PIC configures the FPGA circuit from the IDEPROM status LED is red during configuration After that the FPGA controls the entire pneumatic system through the Pneumatic 1 C bus the Keyboard and Display module with video RAM for MDA Monochrome Display Adapter emulation and Start button amp status LED FPGA circuit also performs measurement data acquisition by using the 10 bit A D chip FPGA makes digital data processing and stores the results in the internal FIFO memory Cell parameters are sent to the DIMM PC by single DMA cycles rev 1 09 cAbacus Service Manual 13 2 1 5 DIMM PC Module The MBA board incorporates a credit card sized PC named DIMM PC The processor on the DIMM PC is a 133MHz Pentium class core with 32Mbytes on board RAM and 32Mbytes on board FlashDisk This is the HDD hard disk drive of the analyzer so instrument software with all user settings calibration database etc is stored on the DIMM PC e DIMMPCOQ is the Trade Mark of Kon

3 - Frank`s Hospital Workshop

Contents

Download Pdf Manuals

Related Search

Related Contents