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1. SRB Experts in Thermostatics CO incubators INCO 108 INCO 108 med INCO 153 INCO 153 med INCO 246 INCO 246 med Manufacturer and customer service MEMMERT GmbH Co KG Postfach 17 20 D 91107 Schwabach AuBere Rittersbacherstr 38 D 91126 Schwabach Germany Fon 49 0 09122 925 0 Fax 49 0 09122 14585 E Mail sales memmert com Internet www memmert com Customer service Fon 49 0 09122 925 128 and 49 0 09122 925 126 E Mail service memmert com For service enquiries please always specify the appliance number on the nameplate see page 15 2014 Memmert GmbH Co KG Edition 11 2014 Subject to modifications memmert About this manual About this manual Purpose and target group This manual describes the setup function operation and maintenance of CO incubators of the types INCO and INCOmed with a chamber volume of 108 153 and 246 litres In this manual the term INCO is used for INCO as well as INCOmed for the purpose of simplification Distinctions are explicitly pointed out This manual is intended for use by the trained staff of the operator in charge of operating and or maintaining the incubator If you are asked to work on the incubator you should read this manual carefully before starting work on the unit Familiarise yourself with the safety regulations Only perform the work that is described in this manual If there is something you don t understand or certain information
2. 5 CO connection to main gas bottle 6 CO connection to reserve gas bottle only for models with CO or premium modules 7 N connection quick release connector only if equipped with O module Design and Function memmert 2 6 1 Electrical connection Observe the country specific regulations when making connections e g in Germany DIN VDE 0100 with residual current device This appliance is intended for operation on an electrical power system with a system imped ance Z__ at the point of transfer service line of a maximum of 0 292 ohm The operator must ensure that the incubator is operated only on an electrical power system that meets these requirements If necessary you can ask your local energy supply company what the system impedance is 2 6 2 Connection of external appliances Only appliances may be connected externally depending on the model USB RS 232 RS 485 Ethernet printer whose interfaces comply with the requirements for safety extra low voltage e g PC 2 6 3 Gas connection The oven can be connected with the supplied compressed air hose via a pressure regulator with gas bottle monitor DIN 8546 to a CO compressed gas bottle or directly to a central CO gas supply For models with the CO or premium modules two gas bottles with quick release connectors can be connected If equipped with the O module an N gas bottle can be connected instead of a second CO gas bottle Fig 4 The pre pressure must no
3. gt coe ts CO calibration at 15 CO content only for models with CO or premium modules For each selected balance point a positive or negative compensation correction value can be set rn M LL IL 15 pe Bag Loe 5 DH e 4 Fig 20 CO calibration example Setting 5 Setthe desired CO balance point in the SETUP see page 44 and set accompanying compensation correction value to 0 0 rh 6 With a reference instrument measure the deviation in the stationary state in the selected CO balance point 7 Set the compensation correction value in the SETUP If the measured reference CO content is too low the compensation correction value must be set with a negative sign 8 Perform a control measurement with the reference instrument 9 The procedure can be performed for the CO balance points 5 10 and 15 15 only for models with CO or premium modules 53 Advanced functions Example CO deviation in chamber load at 10 should be corrected 1 Set the CO balance point in the SETUP to Ce D and set the accompanying compensation correction value to DU 2 With a calibrated reference instrument and at a set CO content of 13 5 an actual CO content of 11 5 is measured 3 Set the compensation correction value in the SETUP for Cie iD to 15 4 The reference instrument should display 10 after the calibration procedure With Ge 5 and Coe 15 further calibrations can be progra
4. The current CO actual value appears on the display and the controller begins to move to the set CO setpoint Depending on the bottle used fumigation is dis J played by the int or N2 icon 6 Adjust the O setpoint Mo We Fr ER Su only for models with O module fon a Turn the push turn control to the left until the O display off i H h flashes Hold down the set key and set the desired O setpoint of 3 0 with the push turn control Release the set key The appliance flashes briefly showing the O setpoint Then the current O actual value appears on the display and the controller begins to move to the set O value The incubator is now running in permanent operation with the set values 5 10 3 Week time switch In this operating mode the appliance switches on and off automatically at the times programmed C1 During the OFF phase of the week time switch the appliance is in standby mode The heating and cooling functions along with the CO and humidity supply are switched off here and the controller display shows the time dimmed The sequence of the week time switch repeats itself each week In total a maximum of 9 time blocks can be programmed consisting of the switching on and switching off times 31 Operation and control memmert Weekday Adjustment range Monday to Sunday Day groups Adjustment range Working days Mo Fr Weekend Sat Sun No switch on time A
5. 1 The log memory of the controller is not modified or deleted by the reading out Printing out log memory see also Chapter Printer on page 43 For a printout the GLP header is also printed automatically and contains the following details Date of printout Period of log gt Consecutive page numbers Serial numbers and appliance name Ifthe printer is not ready e g ink cartridge or paper tray empty no log data will be 1 lost Multiple printouts can also be made since the log memory is not deleted after print Ing 58 memmerf Maintenance and Servicing 8 Maintenance and Servicing 8 1 Cleaning Warning Danger of injury Before any cleaning work pull out the mains plug Regular cleaning of the easy to clean chamber interior prevents build up of material remains which over time could impair the appearance and functionality of the stainless steel chamber To clean the interior the fan cover can be removed by pulling it slightly forwards after the two fixing screws have been screwed out Fig 36 The protective grid of the O sensor can if the model is equipped with a O module be taken off after the fixing screws have been removed Fig 37 The metal surfaces of the chamber can be cleaned with nor mal stainless steel cleaning agents Make sure that no rusty A A Y d objects come into contact with the working chamber or with Fie 36 To take off the the stainless steel housing Rust deposit
6. If requested on the corresponding order the oven can be fitted in the factory with a RS 485 interface instead of a RS 232 C interface This enables the networking of several ovens up to 16 with one computer via a shared two wire cable Fig 34 The chamber must be given a unique device address in the SETUP submenu menu item ADDRESS see page 44 via which the computer communicates with the oven A maximum of 16 devices can be addressed on the RS 485 bus The default setting is ADDRESS D Using this the appropriate incubator RS 485 can be selected and programmed from the PC On leer The computer needs to be equipped with either an RS 485 interface Cy aoe or with an RS 232 RS 485 converter Fig 34 Connecting the wires is done individually with a shielded cable depending on the installa tion site The maximum total cable length is 150 m 220 ohm terminating resistor must be connected to the last device Fig 33 RS 485 Pin allocation interface notoc notoc A notoc notoc notoc not oc not oc cupied cupied cupied cupied cupied cupied cupied 56 memmert Advanced functions Interface converter memmerk memmerk memmert Fig 34 Connecting incubators via the RS485 bus interface 7 7 3 USB interface If several chambers are to be connected to a computer via USB interface an appropriate interface on the computer and a separate cable are required for each chamber The maximum cable length is
7. Premium modules 23 Putting into Operation For incubators with O module Attach the supplied CO pressure hose to the CO gas bottle pressure reducer and to the CO2 In connection on the rear of the incubator with a hose clamp Fig 13 see also page 13 Attach push on the supplied N pres sure hose to the N gas bottle pressure reducer and to the N2 In connection on the rear of the incubator with a hose clamp 4 3 Oxygen cal bration only for models with O module Perform an O calibration before putting into operation see page 54 24 memmert Fig 13 Gas connection for incubators with O mod ule memmert Operation and control 5 Operation and control 5 1 Operating personnel The incubator may only be operated by persons who are of legal age and have received in structions for the incubator Personnel who are to be trained instructed or who are undergo ing general training may only be active on the incubator under the continuous supervision of an experienced person 5 2 Opening the door To open the door turn handle to the right Fig 14 To close turn door handle to the left When the door is opened the CO supply is automati u cally interrupted If the heated outer door is left open for any length of time condensation may form on the glass door 5 3 Loading the incubator Warning When loading the chamber with an Fig 14 Opening and unsuitable
8. Put the covers back on Fig 8 Base Fig 9 Assembly of the foot alignment provisions when two incubators are placed on top of one another 21 Putting into Operation memmert 4 Putting into Operation 4 1 Checks 4 1 1 Checking the temperature sensor Especially strong vibrations during transport could result in the temperature sensors being moved in their holders in the working chamber Check the temperature sensor for its correct positioning and if necessary adjust its position in the holder Fig 10 4 1 2 Check the door and adjust if necessary See page 60 4 2 Connecting 4 2 1 Power supply Caution 1 Observe the country specific regula tions when making connections e g in Germany DIN VDE 0100 with residual current device RCD Observe the connection and power ratings see nameplate The incubator is intended for operation on an electrical power system with a system imped ance Z at the point of transfer service line of a maximum of 0 292 ohms The operator must ensure that the incubator is operated only on an electrical power system that meets these requirements If necessary you can ask your local energy supply company what the system impedance is Connect power cable see Fig 4 on page 13 Fig 10 Temperature sensor 4 2 2 External devices only for models with communication or premium modules Only appliances whose interfaces com
9. control guarantees that setpoint humidity is quickly reached without the use of water trays In the heating up phase the humidity control is initially deactivated Approx 5 minutes after the setpoint temperature is reached the humidification and dehumidification control starts working The setpoint can be set from 40 to 97 rh The humidity setpoint can also be ad justed during the transient state For humidification water is let into the chamber via a dosing pump To avoid the formation of germs the steam is first heated to approx 140 C Dehumid ifcation takes place through the supply of dry air via a sterile filter If no humidity is required in the chamber the humidity control can be deactivated by U setting it to GFF 36 memmert 5 12 Ending operation 1 Pe Ze Switch off appliance To do this press the main switch on the operating panel so that it clicks into place in the appliance see Fig 22 Close the valve s of the gas bottle s Open the door see page 25 Remove the chamber load For appliances with the basic model Remove and empty the water trays Fill water trays and insert them only when the appliance is next used For incubators with a humidity module empty the water tanks if the incubator is not used for several days Fig 22 Operation and control Switching off incubator 37 Warning messages and malfunctions 6 6 1 Warning messages memmert Warning messages and mal
10. damage to electrical parts For this reason sufficient measures should be taken to prevent large clouds of dust or aggressive vapours from developing 2 12 Accessories included For incubators with basic fittings Two INCO 108 or three INCO 153 and 246 sliding shelves P Gas pressure hose gt Water tray one for INCO 108 and 153 incubators two for INCO 246 incubators Sterilisation chipcard Additionally for models with humidity module gt Water supply tank and connection hose Additionally for models with CO or premium module P Second gas pressure hose with quick release connector Additionally for models with O module P Second gas pressure hose with quick release connector memmert Delivery Transport and Setting Up 3 Delivery Transport and Setting Up 3 1 Safety regulations Warning You may injure your hands or feet when transporting and installing the incubator You should wear protective gloves and work shoes you try to lift it on your own If possible only transport the incubator with a fork lift truck or manual pallet jack The incubator may only be moved using a means of transport by persons who have the required qualification for this e g fork lift truck licence This incubator may not be transported Warning EI Because of the weight of the incubator you could injure yourself if with a crane If the incubator has to be carried at least two people are required for models 108 and 153 an
11. is missing ask your superior or get in touch with the manufacturer Do not do anything without authorisation Contents The INCO incubator is available with different fittings as a basic model and with six different additional modules The technical fittings and functional range of the basic model and the individual additional modules are described from page 11 If specific equipment features or functions are available only with one of the additional mod ules this is pointed out in the relevant sections in this manual Due to individual fittings depictions in this manual may be different from the actual appear ance Other documents that you must read for service and repair work see page 59 a separate service manual Storage and Forwarding This instruction manual belongs with the incubator and should always be stored so that those who work on the incubator have access to it It is the responsibility of the operator to ensure that persons who work on or who will work on the incubator are informed as to the wherea bouts of this instruction manual We recommend that it is always stored in a protected loca tion close to the incubator Make sure that the instruction manual is not damaged by heat or damp If the incubator is sold on or transported and then set up again at a different location this instruction manual must go with it Content memmert Content 1 Safety regulations 6 1 1 Terms and icons used ueuueesennnne
12. load poisonous or explosive closing the door vapours or gases may be produced This could cause the chamber to explode and people could be badly in jured or poisoned The chamber may only be loaded with materials test objects which do not form any toxic or explosive vapours when heated up and which cannot ignite If there is any doubt as to the composition of materials they must not be loaded into the incuba tor Caution U Check the chamber load for chemical compatibility with the materials of the incubator see page 13 since considerable damage could otherwise occur to the chamber load the appliance or the surroundings The incubators are not explosion proof they do not comply with workplace health amp safety regulation VBG 24 and are therefore not suitable for drying vaporising and branding paints or similar materials the solvents of which could form an explosive mixture when combined with air Potentially explosive gas air mixtures must not be produced either in the interior of the chamber or in the direct vicinity of the appliance Heavy dust production or aggressive vapours in the chamber or in the vicinity of the appli ance could lead to sedimentation in the chamber interior and as a consequence could result in short circuits or damage to electrical parts For this reason sufficient measures should be taken to prevent large clouds of dust or aggressive vapours from developing The chamber must not be loa
13. only if equipped with O module Design and Function The CO content is determined through an NDIR measur ing system controlled constantly by a microprocessor and displayed digitally in percent gt Adjustment range 0 to 10 for models with CO or premium modules 0 to 20 gt Adjustment precision 0 1 Variation time max 0 1 rh Variation spatial max 0 3 The O content is determined with a long lasting mainte nance free zirconium dioxide sensor constantly controlled by a microprocessor and displayed digitally in percent gt Adjustment range 1 to 20 gt Adjustment precision 0 1 Variation time max 0 1 Variation spatial max 0 3 Fig 6 Dimensions of INCO incubators Design and Function memmert 2 11 Ambient conditions The incubator may only be used in enclosed rooms and under the following environmental conditions Ambient temperature 5 C to 35 C Humidity max 80 not condensing Degree of pollution 2 Altitude of installation max 3 000 m above sea level gt The incubator may not be used in areas where there is a risk of explosions The ambient air must not contain any explosive dusts gases vapours or gas air mixtures The incubator is not explosion proof gt Heavy dust production or aggressive vapours in the vicinity of the appliance could lead to sedimentation in the chamber interior and as a consequence could result in short circuits or
14. or CO limits are crossed when door is open and if gas bottle is empty see page 38 Calibration of temperature humidity CO and O possible on the device without a sepa rate PC see from page 50 L A A A Y A AA 2 3 2 Optionally available additional modules Comfort module Two gas connections with quick release connectors see page 13 gt Automatic switch over of gas bottles Hygiene module gt Electro polished seamless laser welded chamber Communication module gt Logging option of temperature CO2 and relative humidity via computer laptop Internal log memory with 1024 kB as ring memory for all setpoint and actual values errors and settings in real time and with date logging approx 3 months at 1 minute storage interval see page 58 Parallel printer port PCL3 compatible for printing out log data see page 13 and page 43 gt optionally USB Ethernet RS 232 or RS 485 interface see from page 55 CO _ module Extended adjustment range from 0 to 20 3 point calibration 5 10 15 CO O module gt Control of oxygen concentration through the introduction of nitrogen N adjustment range 1 to 20 O not in combination with Comfort or Premium module Premium module Includes comfort hygiene communication and CO modules Humidity module gt Active microprocessor humidification and dehumidification control 40 97 rh memmert Design and Function 2 4 Material For the
15. outer housing MEMMERT uses stainless steel W St No 1 4016 and for the interior stainless steel W St No 1 4301 which stands out through its high stability optimal hygienic properties and corrosion resistance towards many but not all chemical compounds caution for example with chlorine compounds The chamber load for the appliance must be carefully checked with respect to chemical compatibility with the materials mentioned A material resistance table can be requested from the manufacturer 2 5 Electrical equipment gt Operating voltage See nameplate page 15 50 60 Hz gt Current consumption See nameplate page 15 Protection class 1 i e operating insulation with safety earth terminal in accordance with EN 61010 P Protection type IP 20 acc to EN 60 529 P Interference suppressed acc to EN 55011 class B Appliance fuse Fusible link 250 V 15 A quick blow gt The temperature controller is protected with a miniature fuse 100 mA 200 mA at 115 V gt For models with a humidity model the CO controller is protected with a 6 3 A miniature fuse 2 6 Connections Fig 4 Connections on rear of appliance 1 Water connection only for model with humidity module 2 Mains lead 3 USB connection only for models with communication or premium modules alternatively Ethernet RS 232 or RS 485 connection details from page 55 4 Printer connection only for models with communication or premium modules see page 43
16. supply Undo gas bottle connections and close valves of gas bottles Gas bottles may be stored in closed rooms if these are sufficiently well ventilated For appliances with basic fittings Remove empty and clean the water tray s For appliances with the humidity module Undo hose connection of water supply tank empty water tank 9 2 Disposal This product is subject to the Directive 2002 96 EC on Waste Electrical Electronic Equipment WEEE of the European Parliament and of the EU Council of Ministers This appliance has been brought to the market after 13th August 2005 in countries which have already integreated this directive into their national laws It may not be disposed of in normal household waste To dispose please contact your dealer or manufacturer Any appliances that are infected infectious or contami nated with materials that are a hazard to health are excluded from being taken back Please observe the other regulations in this context Before disposing of the appliance please render the door locking mechanism unusable for example to prevent playing children from being locked inside the appliance Note for Germany The appliance may not be left at public or communal recycling or collection points 61 Index Index A Accessories 18 Accidents 9 Acoustic signal 44 Additional modules 12 Adjusting door 60 Advanced functions 43 Ambient conditions 18 Appliance error 39 ASF 48 Automatic temperature mon
17. switch example 1 Setting the week time switch operating mode Hold the SET key down for approx 3 seconds the current C1 operating mode then begins to flash Select the week time switch operating mode with the push turn control while the SET key is held down Release the SET key the control is now in the week time switch operating mode 2 Switch on Mo Fr at 09 30 Mel We Fr Turning the push turn control to the left select the MO FR GM icons group working days Hold down the SET key and set the desired switch on time with the push turn switch to 3 30 3 Switch off Mo Fr at 19 00 Select M FR OFF group working days with the push turn control Hold down the SET key and set the desired switch off time with the push turn switch to 9 00 33 Operation and control memmert 4 Switch on Sa at 10 00 With the push turn control select SAT GM Hold down the SET key and set the desired switch on time with the push turn switch to ON 5 Switch off Sa at 14 00 With the push turn control select SAT GFF Hold down the SET key and set the desired switch off time with the push turn switch to 4400 5 10 5 Operation with PC laptop optional If equipped with the communication or premium module the incubator can optionally be used controlled and programmed with a PC laptop It has corresponding communication interfaces for this purpose see page 13 and page 55 Operation is described in a s
18. to IN1 see also Fig 4 on page 13 35 Operation and control memmert To be on the safe side a freshly filled CO gas bottle should always be used So if the gas in J bottle 1 is used up you connect the opened bottle to INT and the newly filled bottle as a reserve bottle to IN2 The hose connection system used by Memmert shuts off automatically if a connection hose is pulled off You should still always close the stop valve on the gas bottle if a bottle is empty or not connected lights up when gas bottle 1 is active If gas bottle 1 is empty there is an automatic switch over to the reserve bottle lights up after the switch over to gas bottle 2 reserve bottle Switching over to the reserve bottle is marked by a short re peated acoustic signal about 3 seconds long the default after switching on is gas bottle 1 In the following cases a switch over is made from reserve bottle 2 back to the main supply bottle 1 if the reserve bottle is empty P each time after the appliance is switched on after every change of the CO setpoint Humidity limit control A humidity limit control prevents the formation of condensation water in the chamber and at the same time ensures that the setpoint humidity is quickly reached with short recovery times The maximum achievable humidity can be adjusted in the standard model from 88 to 97 rh Active humidity control only for models with humidity module The active humidity
19. unit may only be performed by qualified electricians Warning When loading the chamber with an unsuitable load poisonous or explosive vapours or gases may be produced This could cause the chamber to explode and people could be badly injured or poisoned The chamber may only be loaded with materials test objects which do not form any poisonous or explosive vapours when heated up see also Chapter 2 7 Intended use on page 14 Warning Danger of suffocation In high concentrations CO and N can have a suffocating effect In normal operation the incubator gives off small amounts of CO and if equipped with the O module N to its environment You should therefore ensure that the room in which it is installed is properly ventilated If a gas bottle is not con nected or is empty always close the stop valve or pressure reducer on the bottle Warning High concentrations of CO can cause cold burns or frostbite Avoid contact with CO gas to the eyes and skin Warning CO gas bottles may burst or explode at high temperature Do not use naked flames in the vicinity of the gas bottles Store gas bottles at lower than 50 C in a well ventilated location Prevent water from penetrating as well as backflow into the gas bottles It is essential that you read the safety notes and regulations of the gas suppliers CO and N are not dangerous substances in terms of the German Ordinance on Hazardous Substances GefStoffV You
20. 4 Protection type 9 Connection performance values 5 CE conformity 10 Factory number Design and Function menmenl 2 10 Technical data Model See Fig 6 on page 17 Chamber width A mm Chamber height B mm 480 640 640 Chamber depth C mm 400 500 600 Appliance width D mm 710 630 790 Appliance height E varies 778 920 938 due to adjustable feet mm Appliance depth F includ 590 690 790 ing door handle mm Chamber volume litres 108 153 246 Weight kg 70 90 110 Performance W 1000 1500 2000 Max number of sliding 4 6 2 x 6 6 shelves half size full size Max load per sliding shelf 15 15 15 kg Max load per appliance kg 40 40 60 Temperature Temperature recording by means of Pt100 in a 4 wire circuit Adjustment range Normal mode 20 C to 50 C Sterilisation mode 160 C 4 hours via STERICard Adjustment precision 0 1 C Control range from 8 C above room temperature to 50 C Variation time max 0 1 C at 37 C Variation spatial max 0 3 C at 37 C Humidity The relative humidity in the chamber is measured by a capac itive humidity sensor and displayed digitally in percent The measurement precision of the humidity sensor is 1 rh gt Adjustment range 88 to 97 rh for models with humidity module 40 to 97 rh gt Adjustment precision 1 rh Display range 10 to 98 rh Variation time max 1 rh memmert Model See Fig 6 on page 17 O
21. 5 0 Fax 49 0 9122 145 85 E Mail service memmert com www memmert com 05 11 2014 INCO INCO med englisch 023532
22. 5 m 7 7 4 Ethernet interface LAN 1 192 168 1 233 LAN 2 192 168 1 215 LAN 3 192 168 1 241 Fig 35 Connecting one or more incubators to a network using an Ethernet interface schematic diagram 37 Advanced functions memmert For identification purposes each appliance connected must have its own unique IP address Each chamber is delivered by default with the IP address 192 168 100 100 The program XTADMIN which can be found on the CD ROM provided can be used to change the IP ad dress Setting the IP address is described in a separate manual 7 8 Log memory only for models with communication or premium modules The controller continually logs all relevant measured values settings and error messages at 1 minute intervals The internal log memory is listed as a ring memory i e the oldest log data are always over written automatically with new data The logging function cannot be switched off but is always active The measured data are stored in the controller safe from manipulation Each dataset is stored with a unique times tamp The internal log memory has a size of 1024 kB This corresponds to a storage capacity for about three months in permanent operation If the power supply is interrupted the time of the power cut and the return of voltage are stored in the controller Reading in the log memory to the PC via USB interface For documentation purposes log data can be read out via an interface
23. Intended use of the INCOmed For INCOmed incubators which are subject to the 93 42 EEC guideline Council Directive on the approximation of the laws of the Member States relating to medical devices the intended use is defined as follows The CO2 incubator INCO med is intended for the creation and maintenance of constant envi ronmental conditions for application in the field of in vitro fertilisation IVF especially for the incubation of oocytes spermatozoa and zygotes in special culture dishes for IVF application as well as for gene expression and the biosynthesis of RNA and proteins 2 8 EC Declaration of Conformity You can download the EC declaration of conformity of the appliance online English http www memmert com en service downloads ce statement German http www memmert com de service downloads eg konformitaetserklaerung 2 9 Designation nameplate The nameplate Fig 5 provides information about the appliance model manufacturer and technical data It is attached to the front of the appliance on the right beneath the door see page 10 RB Typ INC 108 F Nr 0109 0088 10 230 V LL A 50 60 Hz 1000 v 8 s DIN12880 Kl 3 1 Nenntemp 50 C g 8 e Schutzart DIN EN 60529 IP 20 memmert GmbH Co KG D 91126 Schwabach FRG Tel 49 0 91 22 92 50 Made in Germany Fig 5 Nameplate 1 Type designation 6 Address of manufacturer 2 Operating voltage 7 Disposal note 3 Applied standard 8 Temperature range
24. chamber load but only for sterilising the inte rior of the appliance The incubator is not a steriliser with respect to the Law on Medical Devices Make sure that the chamber is empty before the sterilisation procedure is started Sliding shelves and removed water trays may also be sterilised To activate the STERICard insert it into the chip card reader see page 10 on the operating panel of the appliance and select the Start icon with the push turn control while the SET key is held down As soon as the sterilisation procedure has been enabled the automatic sterilisation procedure begins Ramp 1 Heating up to 160 C Ramp 2 Hold time 4 hours after setpoint temperature has been reached Ramp 3 Cooling down to 70 C Ramp 4 Wait time of 10 minutes J the automatic sterilisation programme cannot be changed After the sterilisation has been completed STERILISATION Ob is shown in the controller display For documentation purposes the sterilisation procedure is logged on the STERICard and can be read out with a computer laptop 49 Advanced functions memmert 7 5 User ID card optionally available as an accessory The device number of the incubator anda 7 N unique user number are stored in encrypted form on the user ID card Fig 27 The user ID card therefore works only in the appliance with the corre sponding serial number gt To use it insert the card into the chip Name card reader
25. ck temperature limiter The appliance is only operational again after it has cooled down and the error has been eliminated Open the door for one minute and start calibration again If the message appears gain Contact the customer service Contact the customer service 41 Warning messages and malfunctions memmert 6 3 Power failure In case of a power failure the incubator operates as follows In normal and week time switch operating modes After the power supply has been restored operation is continued with the parameters set The time and the duration of the power failure is documented in the log memory For remote operation If there is a power failure in remote operation the appliance starts in the normal operating mode for safety reasons and all setpoint values are changed to safe default values see table The programme can only be continued from the computer The time and duration of the power failure are documented in the log memory Parameters Default value Temperature eu C CO 0 rh OFF O only for models with O module GFF 42 memmerk Advanced functions 7 Advanced functions 7 1 Printer only for models with communication or premium modules Incubators with a communication or premium module are equipped with a parallel printer port as used in computers Standard PCL3 compatible inkjet printers with a parallel port e g HP DeskJet 5550 or HP DeskJet 9xx can be connected to the printer interface on
26. connection on the rear of the appli ance with two hose clamps Fig 11 see also page 13 Warning Danger of suffocation In high concentrations CO and N can have a suffocating effect In normal operation the incubator gives off small amounts of CO and if equipped with the O module N to its environment You should therefore ensure that the room in which it is installed is properly ventilated Warning High concentrations of CO can cause cold burns or frostbite Avoid contact with CO gas to the eyes and skin Warning CO gas bottles may burst or explode at high temperature Do not use naked flames in the vicinity of the gas bottles Store gas bottles at lower than 50 C in a well ventilated location Prevent water from penetrating as well as backflow into the gas bottles It is essential that you read the safety notes and regulations of the gas suppliers Fig 11 Gas connection for incubators with basic For incubators with CO or premium fittings module Two gas bottles can be connected by simply pushing the supplied pressure hoses onto the CO In1 and CO In2 quick release CO Int connections on the rear of the appliance CO In2 Fig 12 see also page 13 Connect the main gas bottle to In1 a re serve gas bottle can be connected to In2 Attach the pressure hose to the gas bottles pressure regulator with hose clamps Fig 12 Gas connection for incubators with CO or
27. d Warning is always used whenever you or somebody else could be injured if you do not observe the accompanying safety regulation Caution is used for information that is important for avoiding damage 1 1 2 Icons used Prohibited sign forbidding an action 9 Do not tilt i Do not lift appliance appliance Explosive Danger of frost atmosphere bite cold burns Gas Warning icons warning of a danger Danger of electrical shock Warning of gas bottles heat hot surfaces Wear gloves ON Wear safety shoes Two or more persons required Po Regulation signs stipulate an action Disconnect the mains plug Observe information in separate manual SO Other icons Important or 1 useful addition al information First Aid Rinse eyes out Information on first aid memmert Safety regulations 1 2 Product safety and dangers Incubators of the INCO type are technically well developed manufactured using high quality materials and tested for many hours in the factory They contain the latest technology and comply with recognised technical safety regulations But there are still dangers involved even when the appliance is used as intended These dangers are described below Warning After removing covers live parts may be exposed You may receive an electric shock if you touch these parts Disconnect the mains plug before removing any covers Any work inside the
28. d at least four people for model 246 Warning The incubator could fall over and seriously injure you Never tilt the incubator and transport it only in an upright position 3 2 Transport The incubator can be transported in three ways with a fork lift truck move the forks of the truck entirely under the incubator on the manual pallet jack gt by carrying to do this at least two people are required for models 108 and 153 and four people for model 246 Read the weight information detailed on page 16 3 3 Delivery The incubator is delivered in cardboard packaging on a cardboard pallet 3 3 1 Unpacking 1 Remove cardboard packaging or cut open carefully along an edge 2 Lift up incubator from pallet and put down on the appliance feet 3 3 2 Checking for completeness and transport damage Check the delivery note to ensure that the delivery is complete Check the inside and outside of the incubator for damage If you notice deviations from the delivery note damage or irregularities do not put the incu bator into operation but inform the haulage company and the manufacturer 3 3 3 Disposing of packaging material Dispose of the packaging material cardboard in accordance with the effective legal disposal regulations for cardboard packaging in your country Delivery Transport and Setting Up memmert 3 3 4 Storage after delivery If the incubator is initially to be stored after delivery Read the storage con
29. d humidity balance point in the SETUP see page 44 and set accompanying compensation correction value to 30 C 2 With a reference instrument measure the deviation in the stationary state in the selected humidity balance point 3 Setting the compensation correction value in the SETUP If the measured reference humid ity is too low the compensation correction value must be set with a negative sign 4 Perform a control measurement with the reference instrument The procedure can be performed with humidity balance points of 20 rh and 90 rh Example Humidity deviation at 90 should be corrected 1 Set humidity balance point in the SETUP to RH OU and set the accompanying compensation correction value to DU rh 2 With a calibrated reference instrument an actual humidity of 88 rh is measured at nor mal operation with a set setpoint humidity of 35 GH 3 Set the compensation correction value in the SETUP for RH OO TC 2 0 SRH 52 memmert Advanced functions 4 The reference instrument should display 90 0 rh after the calibration procedure With RH 26 a further comparison can be programmed at 20 relative humidity Hall compensation correction values are set to 3 3 rh the factory calibration settings 1 are restored 7 6 3 CO calibration Customer side calibration of appliance on controller by means of three CO points gt 005 CO calibration at 5 CO content gt mg CO calibration at 10 CO content
30. ded too tightly so that proper air circulation in the working chamber is guaranteed Do not place any of the chamber load on the floor touching the side walls or right below the ceiling heating ribs of the working chamber To guarantee an opti mal air circulation push in the sliding shelves so that the gaps between the door sliding shelf and rear wall of the chamber are roughly the same size 25 Operation and control memmert 5 4 Inserting water tray s for appliances with basic fittings Fill the water tray with distilled water and push into the lowest slot Fig 15 Number Filling Amount of level in of water water cm for in Itr for trays each tray each tray approx approx 108 1 145 to 2 54 Tto1s5 153 1 1 5 to 2 5 1 to 15 246 2 1 5 to 2 5 1 to 1 5 Fig 15 Inserting water tray s 5 5 Connect gas supply 1 Check that the gas bottle s is properly connected see also page 13 2 Open valve s 5 6 Switch on appliance The incubator is switched on and off by pressing the main switch push turn control on the front of the appliance Switching on press the main switch so that it comes out of the appliance Fig 16 Switching off press the main switch so that it retracts back into the appliance Fig 17 Fig 16 Switching on incubator Fig 17 Switching off incubator 26 memmert Operation and control 5 7 Basic operation The desired parameters are entered on the operating panel of
31. ditions from page 61 3 4 Setup The incubator can be placed either on the ground or on a table work surface When doing this ensure that the appliance is positioned exactly horizontally The installation site must be level and able to reliably carry the weight of the incubator see page 16 Do not place the ap pliance on an inflammable surface A power connection must be available at the installation site in accordance with the connec tion data on the nameplate see page 15 The distance between the wall and the rear of the chamber must be at least 15 cm The clear ance from the ceiling must not be less than 20 cm and the side clearance from the wall not less than 8 cm Fig 7 Sufficient air circulation in the vicinity of the chamber must be guaran teed at all times Fig 7 Minimum clearance from walls and ceiling 20 memmert Delivery Transport and Setting Up 3 4 1 Base accessory The incubator can be placed on a base Fig 8 3 4 2 Stacking frame accessory Two appliances of the same model size can be placed on top of one another To do this foot alignment provisions must be attached to the lower oven Fig 9 1 Remove the housing cover from the lower oven Insert drilling template supplied with the foot alignments into the overturned lid Mark drilling points and drill with a 4 2 mm diameter drill bit Screw the foot alignment provisions to the top of the lid with the screws and nuts sup plied
32. e ASF is automatically disabled temporarily in the example The setpoint is changed from 37 C auf 30 C section D until it has reached the tolerance range of the new temperature setpoint section E 40 C 40 C Ee E E 7 e E E LLL LL MALL Me Ll 34 C 34 C 33 C IMEE EELEE OUUU li li lt le 2 Xe t ANSErTeH AUTO AN te AUTO Etat AUTO ASF alarm Fig 25 Schematic diagram of how the ASF temperature monitoring functions Switching on the automatic temperature monitor Select the AUTO icon with the push turn control Hold down the SET key and set to DT with the push turn control Switching off the automatic temperature monitor Select the AUTO icon with the push turn control Hold down the SET key and set to GFF with the push turn control The tolerance band for the ASF can be set in the SETUP in the Le OS 5 C see page 44 48 memmert Advanced functions 7 4 Sterilisation chipcard For decontamination the incubator is equipped with a STERICard as standard Fig 26 The STERICard starts an automatic and predetermined sterilisa tion sequence Warning The incubator becomes Sterilisation Parameter gt gt gt gt gt very hot inside during 160 C 4 00 h Sterilisation the sterilisation proce Unit ID Process Control Ke Card dure Do no open the door memmert Bi Caution re sterilisation programme is not Fig 26 STERICard intended for sterilising the
33. ennnesennnnennnnnnnnnnnonnnennnnnnonnnennnnnnnnnnennnnnnnnnnensnnnnnnnnensnnnnnnnnnn 6 1 2 Product safety and dangers nnsnnennnnnannnnnnnennnnernnnnenrnnnnnnnnnnnrnnnnrrnnnnrnnnnennnnnennnnneennneeennnne 7 1 3 Requirements of the operating Dersonnel nennen 8 1 4 Responsibility of the owper 8 1 5 Changes and Reie e EE 8 1 6 Behaviour in case of malfunctions and irregularities nan0nnenonnnennennennnnnnnnnnnnnennneeennnne 8 1 7 What to do in case of acchdents 9 1 8 Switching off incubator in an ermerdency 9 2 Design and Function 10 SN NR RT ee EE 10 2 2 TINCT EE 11 23 Optional sr 11 EE u E E 13 2 5 Electricalequipment se u sa ae 13 26 EOAMECHON Seat ee 13 2 7 Intended use 14 2 8 EC Declaration of Containern een 15 2 9 Designation nameplate au 15 2 10 T ns Aa ee E nna ene ee ee 16 2 11 le Uh COMGIUONS sessa ee 18 2 12 Accessories mduded 18 3 Delivery Transport and Setting Up 19 al Me 1216721 EE ee e noe EEE ee 19 Ce en 19 33 DEINER ee cpa sae ceva gnc ee Aare sco lee no ee 19 2A 1 E 20 4 Putting into Operation 22 Al SCHE een ee ee 22 42 CONNECUNG ei ee ee 22 4 3 Oxygen callbraton 24 5 Operation and control 25 5 1 Operating pers nnel uun usie een 25 5 2 Opening TAG door unse ea 25 5 3 Loading the mcubator E 25 5 4 Inserting water tray S ccccccsssssecccecssssseeceecssssseeceeessseeeeeesesssasseeeeensseseeseeeessssseeeeeeseaees 26 5 9 COMMECT GaS EEN 26 56 SWIECHON Applia
34. eparate manual It is delivered with the incubator for BA the relevant models 5 11 During operation Regularly check the water level If necessary add distilled water Warning messages during operation See Page 38 CO mode In the heating up phase the CO controller is initially deactivated The CO intake is inter rupted during this period About 5 minutes after the setpoint temperature has been reached the CO control begins measuring and CO gas is let in to the chamber via a sterile filter the valve on the gas bottle must be open To ensure a homogenous distribution of the CO gas in the interior the gas is piped in above the chamber fan The setpoint can be adjusted in 0 1 steps from 0 to 10 for models with CO or premium modules 0 to 20 After a sterilisation procedure and in cycles every 24 hours an automatic zero balance U adjustment is carried out This automatic zero balance adjustment is completed after a few minutes Displays in CO mode AT UP is shown during the heating up phase of the CO sensor In the CO display CO is shown Ht DOOR IS displayed during the djustment 34 memmert Operation and control After the setpoint temperature has been reached the CO concentration is displayed in depending on the setting N7_ indicates that gas bottle 1 is active Is displayed if the CO concentration exceeds the defined setpoint by at least 1 for more than 3 minutes If the concentratio
35. erating mode settings 28 Operating panel 27 Operating personnel 8 25 Operation 25 Operation with PC laptop 34 Operator 25 Oxygen calibration 45 54 P Packaging material 19 Parameters 27 PC 34 Performance 16 Power failure 42 Power supply 22 Printer 43 Printing out log memory 58 Product safety 7 Pt100 temperature sensor 45 Push turn control 26 Putting into Operation 22 Realtime clock 45 Regular maintenance 59 Ring memory 58 RS 232 55 RS 485 56 memmert 5 Safety regulations 6 Service 60 Servicing 59 60 Setting parameters 27 Settings example normal mode 30 Settings example week time switch 33 Setup 19 20 43 Stacking frame 21 Standard accessories 18 Storage after delivery 20 Storage capac ty 58 Switching off 26 Switching on 26 System impedance 22 T Technical data 16 Temperature calibration 50 51 Temperature comparison 50 Temperature deviation 51 Temperature limiter TB 45 Temperature monitor 48 Temperature monitoring 45 Temperature monitoring TWW 46 Temperature sensor 45 Terms used 6 Tolerance band ASF 44 Transport 19 Transport damage 19 TWW 46 Index U Unpacking 19 USB interface 57 User ID card 50 Ww Warning messages 49 Water connection 22 Water tray s 26 Weekday 44 Week time switch 28 31 Weight 16 What to do in case of ac cidents 9 Y Year 44 63 Memmert GmbH Co KG Postfach 1720 D 91107 Schwabach Tel 49 0 9122 92
36. fter it has cooled down and the error has been elimi nated 7 3 2 Electronic temperature monitoring TWW Temperature monitoring can be adjusted independently of the operating modes The manually set monitoring temperature and the overtemperature control is monitored by an adjustable over undertemperature controller TWW protection class 3 3 acc to DIN 12880 If the manually set monitoring temperature is exceeded the TWW takes over tempera ture control and begins to regulate the monitoring temperature Fig 21 The alarm icon flashes as a warning Emergency operation NANANA Setting MAX Set temperature Controller error Fig 24 Schematic diagram of how the TWW temperature monitoring functions The monitoring temperature must always be set sufficiently high above the maximum H working temperature If the acoustic alarm is switched on in the SETUP the TWW alarm is additionally signalled by an intermittent tone If the SET key is pressed the horn can be temporarily switched off until the next alarm event occurs 46 meEmmE nk Advanced functions Setting Overtemperature limit up to max 10 C above nominal temperature for details of the nominal temperature see nameplate Setting 1 Select the icon with the push turn control 2 Hold down the SET key and set the desired temperature limit with the push turn control e g 385 C Undertemperature limit Adjustment range 10 C below the minimum te
37. functions An intermittent tone is also set off by the warning messages This can be temporarily J switched off by pressing the SET key appliance is opened for longer than 3 minutes Remedy Close the door Error in the temperature control system see also Chapter Temperature monitoring on page 45 38 HME temperature limiter triggered if the temperature limiter responds HDHAHT Overtempera ture alarm limit max ex ceeded if overtemperature protection responds LO ALARIT undertem perature alarm limit min crossed if undertempera ture protection responds H r ALAR TI temperature outside tolerance band if automatic monitoring function responds if the water supply is defective Remedy Switch off the appliance and leave to cool down If the error occurs again after switch ing the appliance back on Switch off the appliance and Contact the customer service Remedy Check the setting of the MAX temperature monitor ing see Chapter Temperature monitoring on page 45 Remedy Check the setting of the MIN temperature monitor ing see Chapter Temperature monitoring on page 45 Remedy Check the setting of the ASF temperature monitoring see Chapter Automatic temperature monitor ASF on page 48 Remedy Check that the water supply hose is properly con nected Fill the water supply tank with distilled water if it is empty memmert Warning messages and mal
38. functions If humidity exceeds the preset setpoint for longer than 30 minutes if the CO supply is defec tive If the CO concentra tion exceeds the defined setpoint by at least 1 for more than 3 minutes if the N supply is defective Remedy Open door for 30 sec and wait to see if the controller steadily adjusts to the setpoint If the error occurs again Contact the customer service Remedy Set CO setpoint value to 0 check stop valve of gas bottle s and ensure that they are properly connected if gas bottle s empty change gas bot tles set CO setpoint to desired value Remedy Open door for 30 sec and wait to see if the controller steadily adjusts to the setpoint If the error occurs again Contact the customer service Remedy check if the N bottle is correctly connected and if the valve is open If that does not solve the problem you should connect a new gas bottle see page 23 6 2 System appliance errors Warning After removing covers live parts may be exposed You may receive an electric shock if you touch these parts Malfunctions requiring in tervention inside the appliance may only be rectified by electricians You must read the separate service manual for the INCO incubator for this Do not try and rectify the error yourself but contact an authorised customer service point for MEMMERT appliances or Contact the customer services department directly of the company MEMMERT see pa
39. ge 2 In case of enquiries please always specify the model and appliance number on the nameplate see page 15 29 Warning messages and malfunctions Nothing shows on the display although the incubator is switched on Appliance cannot be operated No CO display shown in the CO module A icon flashes and RH ETPTY and RH EMPTY Error display E in display 40 Power supply interrupted Appliance fuse or miniature fuse or controller faulty Appliance error Mainboard faulty Incubator locked with user ID card Push turn control faulty Switched mode power supply SP 200 faulty Temperature fuse TWW ASF has re sponded Water supply tank empty Humidity setpoint exceeded CO setpoint is ex ceeded gt Autozero pump faulty CO controller faulty Appliance system error memmert Check power supply and fuse safety switch Contact the customer service and read the service manual Unlock incubator with user ID card see page 50 Contact the customer service and read the service manual Contact the customer service and read the service manual P Increase temperature difference between monitoring and working temperature see page 45 gt Replace Pt100 temperature sensor of monitoring controller if necessary see Service manual Set humidity setpoint to OFF fill up distilled water then reset humidity setpoint back to desired value Open do
40. he compensation correction value in the SETUP If the measured reference tempera ture is too low the compensation correction value must be set with a negative sign 4 Perform a control measurement with the reference instrument 5 Repeat the procedure for the other two balance points if necessary Example The temperature deviation in the chamber load at 35 C should be corrected 1 Set the compensation temperature Ahe f THE SETUP TO 350 C and the accompanying compensation correction value to 55 C 2 With a calibrated reference instrument and at a set setpoint temperature of 35 C in nor mal operation an actual temperature of 34 6 C is measured 3 Set compensation correction value for Ad in the SETUP to 34 C P The reference instrument after the calibration procedure should now display 35 C With Aii another compensation temperature below CHL can be programmed in the same way and with Au3 one lying above this If all compensation correction values are set to DD C the factory calibration settings are 1 restored Si 51 Advanced functions memmert 7 6 2 Humidity calibration The incubator can be calibrated for the individual customer by means of two balance points at 20 and at 90 relative humidity For each selected balance point a positive or negative compensation correction value can be set between 5 and 5 Fig 29 Humidity calibration example Setting 1 Set the desire
41. iagnosis system to quickly locate errors see page 39 All round heater with larger surface area with additional thermal conduction layer see Fig 3 Capacitive humidity sensor Humidity control fresh air via sterile filter ensures that setpoint humidity is quickly reached and guarantees short recovery times while avoiding condensation formation Homogenous atmosphere and temperature distribution through encapsulated turbu lence free ventilation system STERICard for fully automatic sterilisation process control for hot air sterilisation of appli ance including sensors and fan rotor see page 49 vv VY iY Design and Function memmert Digitalised electronic CO control with automatic zero position NDIR measuring system with self diagnosis system and acoustic error display air pressure compensation P Language settings see page 44 gt Alphanumeric text display Integrated week time switch with grup function e g each working day see page 31 Retracting push turn control for simple operation of appliance see page 26 Two separate Pt100 temperature sensors DIN Class A in a 4 wire circuit for control and monitoring Digital monitoring control for overtemperature undertemperature and automatic setpoint following ASF see page 48 Mechanical temperature limiter TB protection class 1 see page 45 Monitoring relay to switch off heater in case of error Optical alarm display Acoustic signal messages if temperature
42. incubator is equipped with the communication or premium module its log data can be read out with a computer laptop For this purpose the incubator has corresponding commu nication interfaces on the rear of the appliance see page 13 To be able to use the RS 232 RS 485 And USB interfaces the incubator must be assigned a unique device address in the SETUP menu item ADDRESS see page 44 via which the computer can communicate with the oven The default setting is ADDRESS D Using this address the ap propriate incubator can be selected from the computer 7 7 1 Communication Interface RS 232 C in accordance with DIN 12900 1 The computer can be connected with a shielded interface RS 232 cable to the 9 pin interface on the rear of the appliance see Fig 31 and page 13 The shielding must be connected to the plug casing 9 pin serial port If the serial port is not used put on the cover included If several ovens are to be connected to a computer via RS 232 C interfaces an appropriate interface on the computer and a separate cable are required for each oven Fig 32 The maximum cable length is 15 m Fig 31 RS 232 C Pin allocation interface notoc RxD TxD notoc GND notoc notoc notoc not oc cupied cupied cupied cupied cupied cupied DD Advanced functions memmert memmert memmert memmert Fig 32 Connecting incubators via the RS 232 C communication interface 7 1 2 Bus interface RS 485
43. irection of the arrow after the two screws 2 at the top and bottom of the door have been undone Afterwards tighten the screws down again Adjusting the door 1 Undo the maggot screw 5 with a jolting motion as it is fixed with locking paint 2 Adjust the door by turning the eccentric 3 with a screwdriver 3 Apply locking paint to the maggot screw and re tighten it The locking plate Fig 39 can also be adjusted in the direction of the arrows after undoing the screw 6 1 Undo screw 6 2 Move locking plate 7 in direction of arrow 3 Tighten the screws again 8 4 Repairs and Service Warning After removing covers live parts may be exposed You may receive an electric shock qualified electricians incubator 60 memmert Fig 38 Adjusting door Upper section of the door hinge Mounting bolts Eccentric tappet Eccentric socket Headless screw MRWNSA P Fig 39 Adjusting locking plate 6 Screw 7 Locking plate if you touch these parts Disconnect the mains plug before remov D ing any covers Any work inside the unit may only be performed by Repairs and service work are described in a separate service manual for the INCO memmert Storage and Disposal 9 Storage and Disposal 9 1 Storage The incubator may only be used under the following conditions Dry and in an enclosed dust free room gt Frost free Disconnected from the power supply and gas
44. isplayed and the incubator 8 begins to heat up to the set temperature Settings for other parameters are made in the same way 27 Operation and control memmert The control returns automatically to the main menu if the push turn key or set key is not operated for approx 30 seconds Setting the temperature Quick adjustment 1 Hold down the SET key and set the desired temperature setpoint with the push turn control 2 Release the SET key The appliance flashes briefly showing the temperature setpoint Then the current temperature appears on the display and the controller begins to move to the set temperature 5 9 Operating modes INCO incubators can be operated in three ways Fig 19 gt Normal mode The incubator runs in permanent operation at the temperature humidity and CO values set on the operating panel Operation in this mode is described from page 29 gt Week time switch The incubator runs at the set values only at certain times Operation in this mode is described from page 31 Interface mode with PC laptop for models with communication or premium modules see from page 55 In addition basic appliance settings can be made SETUP see page 43 and printouts can be made if the appliance is equipped with the communication or premium module PRINT see page 43 Normal Week time Printer Basic appliance operation switch see Page 43 settings see Page 29 see Page 31 see Page 43 Fig 19 Operati
45. itor 48 Base 21 Basic device settings 43 Basic equipment 11 C Calibration 44 50 Chamber load 25 Changes 8 Checks 22 Chipcard 49 Cleaning 59 Clock time 44 CO2 calibration 53 CO2 setpoint 31 Communication address 44 Communication interfaces 55 Compensation temperature 51 Conformity 15 Connecting 22 Connection of external appli ances 14 Connections 13 Conversions 8 Corrective values 44 51 53 Customer service 2 D Date 44 Declaration of conformity 15 Decommissioning 61 Delivery 19 22 Design 10 Dimensions 17 Disposal 61 62 Door 25 Door seals 59 EC Declaration of Conform ity 15 Electrical connection 14 Electrical equipment 13 Electronic temperature moni toring 46 Emergency 9 Ending operation 37 ENDSOUND 44 Errors 39 Ethernet interface 57 Explosion protection 14 External devices 22 Extras 11 F Function 11 G Gas connection 14 Gas supply 26 Hazards 7 How to set the IP address 58 Humidity calibration 52 53 Icons used 6 Intended use 14 Interface cable 43 Interface mode 28 Interfaces 55 Irregularities 8 L Language 44 Laptop 34 Length of lead 57 Loading 25 Log memory 42 43 memmert Main switch 26 Maintenance 59 Malfunctions 8 38 Manufacturer 2 Material 13 Memory 58 Messages 38 Minimum clearances 20 Monitoring temperature 45 Nameplate 15 Normal mode 28 29 O O2 17 O2 module 12 O2 setpoint 31 Operating modes 28 Op
46. key the following parameters can be selected and altered one after another as described in Chapter Basic operation on page 27 Clock time in 24 hr format Conversion to summer time is not automatic but must be done manually Date The controller contains a calendar which automatically accounts for the different lengths of months and for leap years Weekday Year Adjustment range from 2005 TG e i00 Acoustic at programme end ey Orr OF unt Acoustic Signal for alarm e g over undertemperature D oom cm rrr II 20 Setting UFF or D Communication address Adjustment range 0 to 15 see Chapter Communication interfaces on page 55 Tolerance band ASF Adjustment range 0 5 bis 5 C see page 48 Language Setting BERTAN ENGLISH FRANCAS ESPANOL HD TALIA Corrective values CH 1 3 RH20 RH90 customer side calibra tion of temperature and humidity see Chapter Calibration on page 50 AA memmert Advanced functions Oxygen calibration VH D only for models with O module see page 54 Leave Setup Save all settings and leave the SETUP mode The realtime clock which is set in the SETUP contains the date and clock time It is used for logging purposes in accordance with GLP Date and clock time are specified on the log printout On graphical printouts the time axis is labeled with the realtime The clock is battery buffered and independent of the mains connection The integra
47. mmed at 5 and 15 15 only for models with CO or premium modules If all compensation correction values are set to DD the factory CO calibration settings are restored 7 6 4 Oxygen calibration only for models with O2 module In the O calibration the oxygen proportion in the incubator at normal atmosphere is deter mined 1 Switch off the O module if active To do this turn the oo w Fer CR En push turn control to the left until the O display flashes on Hold down the set key and turn the push turn control Bo until D OFF is displayed Release the set key 2 Set CO setpoint value to 0 see page 31 3 Open the oven door and inner glass door in case of four part door remove glass panes Keep the doors open for at least one minute so that a normal atmos phere can form in the chamber Close the doors again 4 In the setup select the item O calibration with the push turn control CHL Ge GFF Hold down the set key and set to CAL Ge Di with the push turn control Release the set key and leave the setup via EXIT Now the oxygen content in the incubator is measured for about one minute In the display CAL Ge ACTIVE can be seen Do not switch off the oven while this is taking place 5 When the calibration is finished the oxygen content determined is shown in the display 54 memmert Advanced functions 7 7 Communication interfaces only for models with communication or premium modules If the
48. mperature of the appliance up to 10 C above nominal temperature of the appli ance for details of the nominal temperature see nameplate The lower alarm limit value cannot be set higher than the top one If no undertemperature limit is required set the lowest temperature 47 Advanced functions memmert 7 3 3 Automatic temperature monitor ASF ASF is a monitoring device that automatically follows the set temperature setpoint within an adjustable tolerance band Fig 25 The ASF is activated if switched on automatically if the actual temperature value reaches 50 of the set tolerance band of the setpoint in the example 37 C 1 5 C reached for the first time section A The activation of the ASF is shown by the brightly lit icon When the temperature moves outside the set tolerance band around the setpoint in the example in Fig 25 37 C 3 C e g if the door is opened during operation section B of illustration the alarm is set off This is shown by the flashing and icons If the acoustic alarm is switched on in the SETUP the ASF alarm is additionally signalled by an intermittent tone If the SET key is pressed the horn can be temporarily switched off until the next alarm event occurs The ASF alarm goes off automatically as soon as 50 of the set tolerance band of the set point in the example 37 C 1 5 C are reached again section C If the temperature setpoint is altered th
49. n is higher the CO display and the A icon flash In this case you should open the door for 30 sec and wait to see if the controller steadily adjusts to the setpoint If the error occurs again contact Customer Service This monitoring function only starts to work once the CO setpoint has been reached LUc EMRPT is displayed if gas bottle 1 and or 2 is are empty In this case you should connect new gas bottles see page 26 The pressure in the gas bottles is a constant approx 57 bar at 20 C ambient temperature It is not possible to determine how full the bottle is through the pressure since the pressure only drops immediately before the bottle is com pletely empty The CO supply is automatically interrupted when the outer doors are opened DOOR DPEN is indicated in the text display is displayed if the N supply is interrupted In this case check if the N bottle is correctly connected and if the valve is open If that does not solve the problem you should con nect a new gas bottle see page 23 Automatic switch over of CO gas bottles only for models with comfort or premium modules The automatic switch over of gas bottles guarantees an uninterrupted supply with CO gas when two independent supply systems are connected gt Gas bottle 1 is always the main supply bottle gt Gas bottle 2 is always the reserve bottle Operation Is only possible with one bottle In this case it must be connected
50. nce eu 26 5 7 Basic operation E 27 5 8 Setting Darameters E 27 33 Operandi MOE ee ee eee ee ee eee ee 28 5 10 Setting the operating mode 28 511 During operatii EE 34 5 12 Ending operation nennen 37 6 Warning messages and malfunctions 38 6 1 Warning messages sessessesessseeeeeerrrrtsssreerrtttnnnststertrtttnnnnsnenrttttnnnnaaenrrttnEnnnnneereee eenn 38 D System appliance errors cccccccsssscccecesssseeeeessceseeeeecscseaeeeeeesscnsaeeeeeseceaaeeeeesssenseeeeesssenaes 39 6 3 PRON CET 42 memmert Content 7 Advanced functions 43 Sex ND Ge 43 7 2 Basic appliance settings Setup 43 7 3 Temperature Monitoring ccccccccceccssssenseeeeceeeeeessscenneeeeeeeeeeessssesnaeseeeeeeeeeesssessnaeseeeeeess 45 7 4 Sterilisation COND CONG ses 49 7 5 User ID card optionally available as an acCCessop 50 ERUN 50 72 2 Comm nicaton len LE 55 Po LOTOO ee ee ee 58 8 Maintenance and Servicing 59 81 Tt le WE 59 82 EEGEN 59 83 BAUM Le ee ee ee ee ee eee R 60 84 Repairsand SONIC Cocos ccc ee een 60 9 Storage and Disposal 61 Ga Ee e EE 61 92 EE 61 Index 62 Safety regulations memmert 1 Safety regulations 1 1 Terms and icons used In this manual certain common terms and icons are used to warn you of dangers or to give you notes that are important in avoiding injury or damage Observe and follow these notes and regulations to avoid accidents and damage These terms and icons are explained below 1 1 1 Terms use
51. ng modes 5 10 Setting the operating mode 1 Hold SET key down for approx three seconds the selected operating mode then begins to flash 2 Select the desired operating mode normal mode week time switch pro gramming mode printer or basic appliance settings setup by turning control with SET key held down e 3 Release the SET key and the selected operating mode is saved 28 memmert Operation and control 5 10 1 Normal mode The appliance runs in this operating mode in permanent operation The desired setpoints for operating the chamber can be selected The settings have an immediate effect on the func tions of the appliance 1 Load incubator see page 25 2 Switch on appliance To do this press the push turn control on the operating panel so that it comes out of the appliance see Fig 16 on page 26 3 Select the normal operating mode CE with the push turn control 4 As described above set the individual parameters with the push turn control and the set key Temperature setpoint Adjustment range 20 C to 50 C Temperature monitoring Adjustment range MIN MAX AUTO see also page 45 Humidity setpoint Adjustment range di A 1 rh For incubators with basic Oo fittings 88 to 91 rh For models with humidity module 5 to 91 rh High levels of air humidity in the interior can only be achieved without condensation if the interior is thoroughly heated Please set the humidity onl
52. ng that the maintenance plan is adhered to and that maintenance and repair work is properly carried out see page 59 ensures for example through corresponding instructions and inspections that the incuba tor and its surroundings are kept clean and tidy is responsible for ensuring that personal protective clothing is worn by operating person nel e g work clothes safety shoes protective gloves vv VY iY 1 5 Changes and conversions No independent conversions or alterations may be made to the incubator No parts may be added or inserted which have not been approved by the manufacturer Independent conversions or alterations result in the EC declaration of conformity see page 15 losing its validity and the incubator may no longer be operated The manufaturer is not liable for any damage danger or injuries that result from independent conversions or alterations or from non observation of the regulations in this manual 1 6 Behaviour in case of malfunctions and irregularities The incubator may only be used when in a flawless condition If you as the operator notice irregularities malfunctions or damage immediately put the incubator out of service see Chapter 1 8 and inform your superiors y You can find information on eliminating malfunctions from page 38 memmert Safety regulations 1 7 What to do in case of accidents 1 Keep calm Act resolutely and with consideration Pay attention to your own safety 2 Switch
53. ntrol 12 Ventilator inlet opening 4 Connections on the rear of the oven 13 Heating ribs see page 13 14 Adjustable feet 5 Heating ribs see page 11 15 Ba beneath door see page 15 6 Pt100 temperature sensors 16 Door knob see page 25 7 Humidity sensor 17 Door 8 Glass door 18 Chip card reader 9 Chamber seal memmert Design and Function 2 2 Function The air in the incubator is heated up by an all round heater with a large surface Fig 3 No 1 The CO and or N gas is introduced into the working chamber via a sterile filter Because CO has a much higher specific weight than air the gas is let into the working chamber above the interior fan 2 The turbulence free interior ventilation 3 ensures a uniform distribution of the gases creating a homogenous atmosphere In the basic version humidification takes place by means of water trays 4 To avoid uncon trolled condensation dosed fresh air is piped into the interior If the appliance is equipped with a humidity module humidification takes place via a hot air generator which allows water to evaporate at a set rate The sterile hot air is let into the interior above the fan and is mixed with the air current 5 af y 4 E gt GE ES aan e E Fig 3 How INCO incubators work 2 3 Optional extras 2 3 1 Basic equipment Electronic fuzzy supported PID process controller with pulse width modulation and per manent performance adjustments and time saving self d
54. off the incubator and close the valves on the gas bottle 3 Call a doctor 4 Initiate first aid measures If available Call a trained first aid helper In case of contact with CO to the eyes and skin Rinse eyes out with water for at least 15 minutes With cold burns rinse with water for at least 15 minutes Cover over in a sterile manner Call a doctor Inhaling CO or N High concentrations can cause suffocation Symptoms may include a loss of mobility and consciousness The victim is not aware of suffocating Low concentrations of CO can cause accelerated breathing and headaches Anyone affected should breathe fresh air using a breathing device independent of recircu lating air Keep the person warm and calm Call a doctor In case of respiratory arrest use artificial respiration If gas is escaping Leave the room immediately warn others and ventilate the room If you re enter the room use an autonomous breathing device independent of ambient air if it has not been estab lished that the atmosphere is harmless 1 8 Switching off incubator in an emergency Push main switch on front side of appliance Fig 1 Close the valves on the gas bottle Fig 1 Switch off incubator by pressing the main switch Design and Function memmer F 2 Design and Function 2 1 Design Fig 2 Design of INCO incubators 1 Controller control panel see page 27 10 Sliding shelf 2 Set key 11 Door seal 3 Push turn co
55. or for 30 sec and wait to see if subsequently the controller steadily ad justs to the setpoint If the error occurs again Contact the customer service Open door for 30 sec and wait to see if subsequently the controller steadily ad justs to the setpoint If the error occurs again Contact the customer service Contact the customer service and read the service manual Contact the customer service and read the service manual memmert Warning messages and malfunctions Error message Curr is displayed for only 10 sec after switching on Chamber fan without function Heating icon w not on AN icon is permanently lit up CAL 0 ERROR Checksum error error when saving setpoint values Miniature fuse or mains adapter faulty gt Ambient tempera ture too high gt Temperature in appliance higher than set setpoint temperature Temperature fuse has responded Fault in oxygen calibra tion Oxygen sensor faulty The error can be rectified just by the controller after a setpoint parameter has again been saved If the error should continue occurring or cannot be rectified Contact the customer service and replace controller see service manual Contact the customer service and read the service manual Setp the appliance up in a cooler room minimum setpoint temperature am bient temp 8 C Wait until appliance has cooled down Switch off appliance and leave to cool down gt Che
56. ply with the requirements for safety extra low voltge e g PC laptop printer may be connected to the connections on the rear of the incubator see Fig 4 on page 13 Which devices may be connected depends on the chosen model module variant Communication interfaces described in detail from page 55 4 2 3 Water connection only for models with humidity module For steam creation use only demineralised water aqua dem in accordance with VDE 0510 DIN EN 50272 regulations must be strictly adhered to production conductivity gt 5 lt 10 uS cm Battery water in accordance with VDE 0510 is available in larger chemist s shops super markets hardware stores and in the wholesale trade The standard VDE 0510 DIN EN 50272 has to be explicitly specified on the label Otherwise possible calcification in the steam generators steam piping and peristaltic pumps could affect the operability of the device The water used must have a pH value between 5 and 7 and be chlorine free 22 memmert 1 Putting into Operation Fill up the supplied water supply tank canister with distilled water and place behind next to the incubator Attach the supplied hose the quick release connections to the canister and the water sup ply H O on the rear of the appliance see Fig 4 on page 13 4 2 4 Gas connection For incubators with basic fittings Attach the supplied pressure hose to the gas bottle pressure regulator and to the CO
57. ppliance not switched on on this day Switch on time on Adjustment range DEDO TO 23 59 HOURS Switch off time off One minute beyond the switch on time up to H H if if h By turning further to the right parameters temperature humidity setpoints etc can be selected as in the normal operating mode If no settings temperature setpoint etc are made for the ON phase the controller takes J over the values from the normal operating mode For reasons of safety you should always check that only one switch one time is programmed in the desired time blocks and days By turning further to the right parameters temperature setpoint etc can be selected as in the normal operating mode If the controller is in standby mode or the week time switch is in the ON phase the tempera ture setpoint can be directly accessed by briefly pressing the SET key By turning the control to the right you are returned to temperature monitoring humidity and CO setting By turning to the left you come back to the settings for the individual time blocks 32 memmert Operation and control 5 10 4 Settings example week time switch From Mo Fr workdays group the appliance should switch on at 9 30 and switch off at 19 00 In addition it should work on Saturday from 10 00 to 14 00 Fig 21 ie JE 2 ST x I te c gt fon oD 9 30h 19 00h 9 30h 19 00h 9 30h 19 00h Fig 21 Operation with week time
58. s lead to an infection Si e Eeer e of the stainless steel screws If rust spots should appear on the surface of the working BB due to impurities immediately clean and polish the affected area Do not clean the operating panel the plastic input module and other plastic parts of the chamber with caustic or solvent based cleaning agents 8 2 Regular maintenance Monthly For models with O module Perform oxygen calibration see page 54 Every three months gt Check that door seals fit tightly adjust door if necessary see page 60 gt In permanent mode Grease the moving parts of the doors hinges and lock with thin silicone grease and check that the hinge screws are not loose Annually gt Grease the moving parts of the doors hinges and lock with thin silicone grease and check that the hinge screws are not loose gt Check that door seals fit tightly adjust door if necessary see page 60 Fig 37 O sensor 59 Maintenance and Servicing 8 3 Adjusting door A well closing door is indispensable for incubators On Memmert appliances the tight closing of the door is optimally guaranteed by a chamber seal and a door seal see also page 10 In permanent operation it is possible that the flexible seal material will begin to sag To ensure that the door closes exactly despite this an adjustment may be necessary Fig 38 The top section 1 of the door hinge can be moved slightly in the d
59. see page 10 on the appli access ance operating panel ID en car If the user ID card has been inserted the L menu item D LECK also appears in the E MEMMEr J SETUP menu If the setting is set to D l the appliance is locked against all altera Fig 27 User ID Card tions once the chip card is removed Locking with the user ID card is displayed with the lit up icon Gg on the operating J Pane Every login process with the user ID card is logged in the internal Flash memory of the control ler 7 6 Calibration 7 6 1 Temperature calibration The incubator can be calibrated customer specifically using three calibration temperatures of your choice CAL Temperature calibration at low temperature to 30 C HO Temperature calibration at medium temperature from 30 C to 38 C gt CALI Temperature calibration at high temperature over 38 C For each selected balance point Fig 28 a positive or negative compensation correction value can be set between 2 9 C and 2 9 C There must be a difference of at least 10 C between each of the individual balance points a u 2 Fig 28 Temperature calibration example 50 memmerk Advanced functions Setting 1 Set compensation temperature in the SETUP see page 44 and set accompanying com pensation correction value to 45 C 2 With a reference instrument measure the deviation in the stationary state in the selected compensation temperature 3 Set t
60. should nevertheless familiarise yourself with the applicable safety regulations prior to handling such gas bottles Safety regulations memmert 1 3 Requirements of the operating personnel The incubator may only be operated and maintained by persons who are of legal age and who have received instructions for the incubator Personnel who are to be trained instructed or who are undergoing general training may only be active on the incubator under the con tinuous supervision of an experienced person The incubator may only be transported by persons fork lift truck manual pallet jack who are trained for this work and who know the corresponding safety regulations Repairs may only be performed by qualified electricians In this case the regulations in the separate service manual must be observed 1 4 Responsibility of the owner The owner of the incubator is responsible for the flawless condition of the incubator and for the incubator being oper ated in accordance with its intended use see page 14 is responsible for ensuring that persons who are to operate or service the incubator are qualified to do this have received instructions about the incubator and are familiar with this operating manual must know about the applicable regulations requirements and work protection regula tions and train staff acordingly is responsible for ensuring that unauthorised persons have no access to the incubator is responsible for ensuri
61. t exceed 1 2 bar A value between 0 8 and 1 bar is considered to be an ideal value 2 6 4 Water connection For models with a humidity module the incubator can be connected with the supplied hose to the also supplied water supply tank 2 7 Intended use INCO incubators may only be used for incubating cell cultures or similar Any other use is improper and may result in hazards and damage The incubators are not explosion proof they do not comply with workplace health amp safety regulation VBG 24 The chambers may only be loaded with materials and substances which cannot produce any toxic or explosive vapours at temperature ranges up to 50 C and which themselves cannot explode burst or ignite The incubators may not be used for drying vaporising and branding paints or similar materi als the solvents of which could form an explosive mixture when combined with air If there is any doubt as to the composition of materials they must not be loaded into the incubator Po tentially explosive gas air mixtures must not be produced either in the interior of the chamber or in the direct vicinity of the appliance The incubator may not be used for sterilisation purposes It is not a steriliser with respect to the Law on Medical Devices Only gas bottles with a pressure regulator may be connected to the gas connections of the oven Introducing other gases or materials than CO or N is not permitted memmert Design and Function 2 7 1
62. ted Lithium battery of the type CR 2032 has a lifetime of approx 10 years 7 3 Temperature monitoring The monitoring temperature is measured via a separate Pt100 temperature sensor in the chamber interior The monitoring unit is used to protect the chamber load and as a protection for the appliance and surroundings The appliance has a double overtemperature fuse mechanical electronic in accordance with DIN 12880 Fig 23 Temperature monitoring display 1 Visual alarm icon Lit up TB alarm Flashing TWW alarm ASF alarm Undertemperature limit Automatic temperature monitor ASF see page 48 Overtemperature protection TWW TWB see page 46 Response temperature Acoustic alarm icon Ohn BR GN 7 3 1 Mechanical temperature monitoring temperature limiter TB The incubator is equipped with a mechanical temperature limiter TB protection class 1 in ac cordance with DIN 12880 If the electronic monitoring unit should fail duiring operation and the factory preset maxi mum temperature is exceeded by approx 20 C the temperature limiter as the final protec tive measure switches off the heating permanently As a warning the icon AN lights up 45 Advanced functions memmert Error rectification after the TB has been triggered 1 Switch off appliance and leave to cool down 2 Contact the customer service and have the error rectified e g replace temperature sen sor The appliance is only operational again a
63. the controller on the front of the appliance Fig 18 Basic settings as well as those for time and printing can also be made here In addition programmed and current parameters are displayed as well as warning mes sages Fig 18 Operating panel 1 Time display 9 nur water supply tank empty 2 Display appliance locked with user ID 10 Humidity display card see page 50 11 Gas bottle 1 active 3 Display appliance is heating up 12 Gas bottle 2 active 4 Sterilisation mode see page 49 13 CO display 5 Temperature display 14 Display appliance is humidifying 6 Alarm display 15 Operating mode SEN see page 28 7 Monitoring temperature display 16 Text display O display O display only if see page 45 equipped with O module 8 Horn All operating functions are selected by turning the push turn control to the left or right and adjusted by turning this with the SET key held down 5 8 Setting parameters In general all setting actions on the operating panel described on the following pages are made in the same way 1 You select the desired parameter with the push turn control menu item e g temperature then all other parameters go dark and the selected one flashes y 2 With the SET key held down set the desired value e g 315 C with the Wi push turn control o _ 3 Release the SET key and the set value is saved The display briefly shows the set value flashing The current temperature is d
64. the rear of the appliance see page Make sure that a shielded interface cable is used The shielding must be connected to the plug casing The controller has an internal log memory see page 58 The log data can be printed out in this mode via the connected printer If a colour printer is connected the various graphs are printed out in colour For a printout the GLP header is also printed automatically containing the following details Date of printout Period of log gt Consecutive page numbers P Serial numbers and appliance name Running the printing function Select the PRINT operating mode with the push turn control as described on page 28 By an turning the push turn control and holding down the SET key the following parameters can be selected and altered one after another as described in Chapter Basic operation on page 27 Querying the date of the first print page Querying the date of the last print page Starting graphical printout Leaving the print menu and returning to the main menu 7 2 Basic appliance settings Setup Setting options may vary depending on the appliance model In this operating mode the basic settings for the appliance can be made The time date day and year are set here along with the settings for the horn the address allocation the monitoring units and the calibration 43 Advanced functions memmert By turning the push turn control and holding down the SET
65. urn control to the right until the monitor ing temperature and the MIN or MAX icon flashes Hold down the SET key and with the push turn control set the overtemperature limit to 38 5 C and the undertem perature limit to 355 C Turn the push turn control to the right until the monitoring temperature and the AUTO icons flash Hold down the SET key and set to Of with the push turn control The tolerance band is set in the SETUP menu see page 44 30 memmert Operation and control 4 Setting the humidity setpoint High levels of air humidity in the interior can only be achieved without condensation if the interior is thoroughly heated Please set the humidity only when the temperature in the interior has been stable for at least 30 minutes Turn the push turn control to the right until the humid ity display flashes Hold down the SET key and set the desired humidity setpoint of 96 0 rh with the push turn control After releasing the SET key the humidity setpoint briefly flashes The current humidity value appears on the display and the controller begins to move to the set value The humidification process is indicated by the 9 icon only for models with humidity module 5 Setting the CO setpoint Turn the push turn control to the right until the CO dis play flashes Hold down the SET key and set the desired CO setpoint of 5 0 with the push turn control The appliance flashes briefly showing the CO setpoint
66. y when the temperature in the interior has been stable for at least 30 minutes CO setpoint ee range D bis 15 or models with CO or premium modules 0 to 20 8 to 20 O setpoint Melo we J Th E Fr Wa Ws only for models with O mod on EE ule m AJuh Adjustment range f EL to d d f 1 29 Operation and control memmert 5 10 2 Settings example normal mode With a 5 CO content a 3 oxygen content and a humidity of 96 the appliance should heat up to 37 C The monitoring function should respond at 38 5 C Fig 20 wech CH 2 0 gt oO 3S E Bes Kn gt 60 D E gt T N oO Fig 20 Example for normal mode 1 Setting the normal operating mode Hold SET key down for approx 3 seconds the current operating mode then begins to flash Select the CL operating mode with the push turn con trol while the SET key is held down After you let go of the SET key the control is in the normal operating mode OD me 2 Setting the temperature setpoint Hold down the SET key and set the desired temperature setpoint of 31 5 C with the push turn control Release the SET key the appliance will briefly flash show ing the temperature setpoint Then the current tempera ture appears on the display and the controller begins to move to the set temperature of 37 0 C gt Heating up is indicated by the icon 3 Setting the monitoring temperature Turn the push t

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