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Agilent 1200 Series Vacuum Degasser

1. 6 1200 Series VD User Manual Introduction 1 How the Electronics Work The vacuum degasser has two different normal operation modes and a continuous mode In operation mode 1 the vacuum degasser works around a defined set point 115 Torr Due to environmental conditions it is possible that the vacuum degasser cannot reach the pre defined set point Under this condition the operation mode 2 becomes active and the vacuum pump is activated in defined time intervals vacuum level 115 to 190 Torr In case of a malfunction of the vacuum degasser vacuum level above 190 Torr the instrument is turned into the error mode Pressure Ambient Error Limit Operation Mode 1 time Figure 2 Operation Modes of the Vacuum Degasser The main function of the vacuum degasser control assembly is to control the vacuum pump and to check the vacuum in the vacuum container The power section of the control assembly comprises a switching power supply that generates 24 V from line voltage The 24 V is used to drive the vacuum pump and the solenoid valve The electronic control circuit uses 12 V which is generated from the 24 V The pressure sensor is connected to the vacuum chamber and checks for the correct vacuum in the system 1200 Series VD User Manual 7 1 Introduction The amplifier and comparator circuit determines the working range of the vacuum that has to be built up When the vacuum degasser is turned on and the vacuu
2. 32 131 of Ambient Non operating Temperature 40 70 C 4 158 F Humidity lt 95 at 25 40 C 77 104 F Non condensing Operating Altitude Up to 2000 m 6500 ft Non operating Altitude Up to 4600 m 14950 ft For storing the instrument Safety Standards IEC CSA UL Installation Category II Pollution Degree 2 1 This temperature range represents the technical specifications for this instrument The mentioned tem peratures may not be suitable for all applications and all types of solvents Unspecified Conditions Operating the instrumentation under conditions other than their intended use might result in a potential safety hazard or might damage the instrumentation gt Never operate your instrumentation under conditions other than specified by the vendor 1200 Series VD User Manual 15 2 16 Site Requirements and Specifications The Agilent 1200 Series vacuum degasser has been tested for evaporation of solvents into the atmosphere by an independent institute with approved methods The tests were performed with Methanol BIA Nr 7810 and Acetonitrile NIOSH Nr 1606 Evaporation of these solvents into the atmosphere when operating the degasser was below the limits of detection 1200 Series VD User Manual Site Requirements and Specifications 2 Performance Specifications Table2 Performance Specifications Agilent 1200 Series Vacuum Degasser Type Specification Maximum flow rate 10 ml min
3. Yellow Power on High NC 7 Red Ready High NC 8 Green Stop Low NC 9 Black Start request Low 1200 Series VD User Manual Identifying Cables 8 Agilent 1100 1200 to 3392 3 Integrators Connector01046 60206 Pin 3392 3 Pin Agilent SignalName Active 1100 1200 TTL 3 1 White Digital ground NC 2 Brown Prepare run Low 11 3 Gray Start Low NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6 Yellow Power on High 9 7 Red Ready High 1 8 Green Stop Low NC 9 Black Start request Low Agilent 1100 1200 to 3394 Integrators Connector01046 60210 Pin 3394 Pin Agilent Signal Name Active 1100 1200 TTL 9 1 White Digital ground NC 2 Brown Prepare run Low 80 15 s 3 3 Gray Start Low NC 4 Blue Shut down Low e o NC 5 Pink Not connected 16 zi NC 6 Yellow Power on High O 5 14 7 Red Ready High 6 8 Green Stop Low 1 9 Black Start request Low 13 15 Not connected 1200 Series VD User Manual 69 8 Identifying Cables START and STOP are connected via diodes to pin 3 of the 3394 connector Agilent 1100 1200 to 3396A Integrators Connector03394 60600 Pin 3394 NC ca NC o O O s NC O oO s s O s O e NC 5 14 1 NC 13 15 Pin Agilent 1100 1200 1 White 2 Brown w Gray Blue Pink Yellow Red Green Black Sig
4. 12 1200 Series VD User Manual Site Requirements and Specifications 2 Power Cords Different power cords are offered as options with the module The female end of each of the power cords is identical It plugs into the power input socket at the rear of the module The male end of each of the power cords is different and designed to match the wall socket of a particular country or region Electric Shock The absence of ground connection and the use of an unspecified power cord can lead to electric shock or short circuit gt Never operate your instrumentation from a power outlet that has no ground connection gt Never use a power cord other than the Agilent Technologies power cord designed for your region Use of unsupplied cables The use of cables which haven t been supplied by Agilent Technologies can lead to damage of the electronic components or personal injury gt Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations CAUTION Unaccessable power plug In case of emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable 1200 Series VD User Manual 13 2 Site Requirements and Specifications Ben
5. left and right CAUTION Wrong assembly In case you insert the left or right side in the opposite position you may not be able to remove the side from the top part gt Take care not to mix up left and right side The cover kit contains all parts but it is not assembled 1 Place the top part on the bench and insert the left and 2 Replace the cover right side into the top part Front Next Steps 3 Replace the vacuum degasser in the stack and reconnect the cables and capillaries 4 Turn ON the vacuum degasser 1200 Series VD User Manual 57 6 Maintenance Checking and Changing the Power Fuses When Tools required Parts required 58 Ifthe vacuum degasser appears dead Test meter if available Part number Description 1 2110 0458 Fuse 500 mA 1 Switch OFF the power switch at the front of the instrument 2 Remove the power cable from the power connector at the rear of the instrument 3 Press down the clip of the fuse holder and pull out of the power socket Fuse holder Figure 12 Fuse Holder and Clip 4 Remove the fuses from the fuse holders Ensure the fuse wires inside the fuses are not broken If a test meter is available check the resistance of each fuse A good fuse shows a low resistance approximately 0 Ohm 6 Ifa fuse is defective wire broken or high resistance insert a new fuse 7 Reinsert the fuse holders and the power cable 8 Switch ON the power
6. per channel Number of channels 4 Internal volume per channel Typically 12 ml per channel Materials in contact with solvent PTFE PEEK pH range 1 14 Analog output AUX For pressure monitoring range 0 3 V The G1322 Vacuum Degasser has been tested for evaporation of solvents into the atmosphere by an independent institute with approved methods The tests were performed with Methanol BIA Nr 7810 and Acetonitrile NIOSH Nr 1606 Evaporation of these solvents into the atmosphere when operating the degasser was below the limits of detection 1200 Series VD User Manual 17 2 Site Requirements and Specifications Performance Specifications 18 1200 Series VD User Manual 1200 Series VD User Manual 3 Installing the Vacuum Degasser Unpacking the Vacuum Degasser 20 Damaged Packaging 20 Delivery Checklist 20 Accessory Kit Contents 21 Optimizing the Stack Configuration 22 Installing the Vacuum Degasser 24 Flow Connections to the Vacuum Degasser 27 Operational Hints for the Vacuum Degasser 31 Priming the Degasser 31 Priming with a Syringe 32 Priming with the Pump 33 Transporting the Vacuum Degasser 34 ee Agilent Technologies 3 Installing the Vacuum Degasser Unpacking the Vacuum Degasser 20 Damaged Packaging Upon receipt of your vacuum degasser inspect the shipping containers for any signs of damage If the containers or cushioning material are damaged save them until the contents have been checked for complet
7. 00 Series VD User Manual ready condition 48 rear of the vacuum degasser 25 remote cable 66 68 RS 232 cable 67 S safety class 76 safety general information 76 76 symbols 79 screwdriver pozidriv 1 54 screwdriver pozidriv 1 59 shipping container 20 site requirements 12 snap fastener 28 solvent cabinet 54 solvent filters checking 39 cleaning 39 prevent blocking 39 solvent outlet tubes 27 solvent bottle 27 solvent cabinet 27 27 solvent information 41 sound emission 82 specification performance specifications physical 15 stack configuration 22 status indicator 47 status light pipe 63 status light pipe 59 syringe adapter 21 32 34 syringe 21 27 T tool 28 mounting tool 28 top cover removing and refitting 54 transportation 34 V vacuum pump 6 voltage range 15 voltage selector 12 volume per channel 17 wW wall socket 13 waste tube 21 weight 15 15 when to use a degasser 43 i 85 www agilent com In This Book This manual contains user information about the Agilent 1200 Series vacuum degasser The manual describes the following e introduction e site requirements and specifications e installing the vacuum degasser e using the vacuum degasser e troubleshooting and diagnostics e maintenance e parts and materials for maintenance e identifying cables e appendix Agilent Technologies 2006 2007 2008 Printed in Germany 11 08 G1322 90011 fe Agilent Tec
8. Agilent 1200 Series Vacuum Degasser User Manual SEE Agilent Technologies Notices Agilent Technologies Inc 2006 2007 2008 No part of this manual may be reproduced in any form or by any means including elec tronic storage and retrieval or translation into a foreign language without prior agree ment and written consent from Agilent Technologies Inc as governed by United States and international copyright laws Manual Part Number 61322 90011 Edition 11 08 Printed in Germany Agilent Technologies Hewlett Packard Strasse 8 76337 Waldbronn Research Use Only Not for use in Diagnostic Procedures Warranty The material contained in this docu ment is provided as is and is sub ject to being changed without notice in future editions Further to the max imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms i
9. LC or Signal Distribution Module Connector01046 60202 Pin HP 1090 Pin Agilent SignalName Active 1100 1200 TTL 1 1 White Digital ground NC 2 Brown Prepare run Low 4 3 Gray Start Low I A E 7 4 Blue Shut down Low si 8 5 Pink Not connected l NC 6 Yellow Power on High 3 7 Red Ready High 6 8 Green Stop Low NC 9 Black Start request Low Agilent 1100 1200 to General Purpose Connector01046 60201 Pin Universal Pin Agilent Signal Name Active 1100 1200 TTL 1 White Digital ground A Q 1 oo 2 Brown Prepare run Low o0 or a KEY 3 Gray Start Low oo BE DE 4 Blue Shut down Low oo ae 5 Pink Not connected Oo 55 6 Yellow Power on High 5 O 15 7 Red Ready High 8 Green Stop Low 9 Black Start request Low 72 1200 Series VD User Manual Identifying Cables Auxiliary Cable One end of this cable provides a modular plug to be connected to the Agilent 1100 Series vacuum degasser The other end is for general purpose Agilent 1100 Series Degasser to general purposes Connector G1322 81600 Color Pin Agilent Signal Name 1100 a White 1 Ground n Brown 2 Pressure signal Green 3 Yellow 4 Grey 5 DC 5VIN Pink 6 Vent 1200 Series VD User Manual 73 8 Identifying Cables Cable Overview 74 1200 Series VD User Manual 1200 Series VD User Manual 9 Appendix General Safety Information 76 The Was
10. Waste outlet Serial number Figure 6 Front of the Vacuum Degasser 1200 Series VD User Manual 25 3 26 Installing the Vacuum Degasser 5 Press in the power switch to turn on the vacuum degasser The power switch stays pressed in and a green indicator lamp in the power switch is ON when the vacuum degasser is turned ON When the line power switch stands out and the green light is OF the vacuum degasser is turned OFF 1200 Series VD User Manual Installing the Vacuum Degasser 3 Flow Connections to the Vacuum Degasser Parts required Description Vacuum degasser 1 Solvent cabinet including solvent bottles filled with solvent and bottle head assemblies 1 1 Solvent outlet tubes 1 Syringe with adapter Preparations Install the vacuum degasser WARNING Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary or tube fittings solvents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Place solvent cabinet with the bottle s on top of the vacuum degasser 1200 Series VD User Manual 27 3 Installing the Vacuum Degasser 2 Remove the front cover by pressing the snap fasteners on both sides Figure 7 Removing the Front Cove
11. ch Space The vacuum degasser dimensions and weight see Table 1 on page 15 allow to place the vacuum degasser on almost any desk or laboratory bench It needs an additional 2 5 cm 1 0 inches of space at either side and approximately 8 cm 3 1 inches at the rear for the circulation of air and electric connections If the bench should carry a complete Agilent 1200 Series system make sure that the bench is designed to carry the weight of all the modules Environment Your vacuum degasser will work within specifications at ambient temperatures and relative humidity as described in Table 1 on page 15 CAUTION Condensation within the vacuum degasser Condensation will damage the system electronics gt Do not store ship or use your vacuum degasser under conditions where temperature fluctuations could cause condensation within the vacuum degasser gt If your vacuum degasser was shipped in cold weather leave it in its box and allow it to warm slowly to room temperature to avoid condensation 14 1200 Series VD User Manual Site Requirements and Specifications 2 Physical Specifications Table 1 Physical Specifications Type Specification Comments Weight 7 kg 15 4 Ibs Dimensions width x depth x height 345 x 435 x 80 mm 13 5 x 17 x 3 1 inches Line Voltage 100 120 or 220 240 VAC 10 Wide ranging capability Line Frequency 50 or 60 Hz 5 Power consumption 30 W Maximum Ambient Operating Temperature 0 55 C
12. condition exists when the vacuum degasser detects an internal defect that does not allow the vacuum to be built up in a certain time frame approximately 8 minutes Internal Leak or Electronic Failure If the error LED is on there is either an internal leak in the vacuum system or an electronic failure In case of an internal leak it is possible that solvent may enter the vacuum chamber and solvent may leak into the waste drain gt To prevent any damage of the vacuum degasser switch off the vacuum degasser and remove the solvent bottles from the solvent cabinet to stop any gravity caused flow of solvent into the vacuum chamber 48 1200 Series VD User Manual 1200 Series VD User Manual aisienare Introduction to Maintenance and Repair 50 Simple Repairs Maintenance 50 Warnings and Cautions 50 Using the ESD Strap 52 Cleaning the Instrument 53 Removing and Refitting the Top Cover 54 Assembling the Main Cover 57 Checking and Changing the Power Fuses 58 Exchanging the Status Light Pipe 59 Agilent Technologies 49 6 Maintenance Introduction to Maintenance and Repair Simple Repairs Maintenance The vacuum degasser is designed for easy repair The most frequent repairs such as exchanging power fuses and status light pipes can be performed by the user but require opening the main cover of the vacuum degasser These repairs are described in this chapter Warnings and Cautions The following procedure
13. connecting tube 21 control circuit 6 cover 62 D dead volume 22 delay volume 22 delivery checklist 20 dimensions 15 15 E electronics how does itwork 7 electrostatic discharge ESD 51 environment 12 14 error condition 48 exchanging status light pipe 59 external contact cable 6 7 F flow connections 27 27 flowrate 17 frequency range 15 front ofthe vacuum degasser 25 fuses 12 G GPIB cable 25 GPIB cable 67 H highest injection precision 36 highest retention time reproducibility 36 humidity 15 information on sound emission 82 installation 11 19 24 27 interface cable 24 internalleak 48 internet 83 introduction to the degasser 6 L laboratory bench 14 LAN cable 67 line frequency 15 line voltage 15 M main cover assembling 57 materials in contact with solvent 17 1200 Series VD User Manual Index maximum flow rate 17 maximum sensitivity 36 mounting tool 21 mounting tool 28 28 non operating altitude 15 number of channels 17 0 operating altitude 15 operational hints 31 P parts accessory kit 64 cover 62 damaged 20 light pipes 63 missing 20 performance specifications 17 pHrange 17 power cable 54 power consumption 15 15 power light pipe 63 power supply lamp 47 power supply 12 power switch 24 power input socket 13 pressure sensor readings 25 pressure sensor 6 priming withapump 31 33 with a syringe 31 principle of operation 6 radio interference 81 12
14. d Electronic Equipment WEEE Directive 2002 96 EC 80 Radio Interference 81 Sound Emission 82 Agilent Technologies on Internet 83 4 1200 Series VD User Manual 1200 Series VD User Manual 1 Introduction Introduction to the Vacuum Degasser 6 How the Electronics Work 7 BE Agilent Technologies 1 Introduction Introduction to the Vacuum Degasser The Agilent 1200 Series vacuum degasser model G1322A comprises a 4 channel vacuum container including 4 tubular plastic membranes and a vacuum pump When the vacuum degasser is switched on the control circuit turns on the vacuum pump which generates a partial vacuum in the vacuum container The pressure is meassured by a pressure sensor The vacuum degasser maintains the partial vacuum by turning on and off the vacuum pump depending on the signal from the pressure sensor The LC pump draws the solvents from their bottles through the special tubular plastic membranes of the vacuum container As the solvents pass through the vacuum tubes any dissolved gas in the solvents permeates through the membranes into the vacuum container The solvents will be almost completely degassed when leaving the outlets of the vacuum degasser Sensor ee i cum circui Pump u J r L 5 _ OO ID 4 Tubular OY plastic O membranes CY N j Solvent as MARI Y Vacuum container LI Figure 1 Overview only one of the four solvent channels is shown
15. dling electronic boards and components 1 Unwrap the first two folds of the band and wrap the exposed adhesive side firmly around your wrist 2 Unroll the rest of the band and peel the liner from the copper foil at the opposite end 3 Attach the copper foil to a convenient and exposed electrical ground Figure 11 Using the ESD Strap 1200 Series VD User Manual Maintenance 6 Cleaning the Instrument Liquid dripping into the electronic compartment of your module Liquid in the module electronics can cause shock hazard and damage the module gt Do not use an exessively damp cloth during cleaning gt Drain all solvent lines before opening any fittings The vacuum degasser case should be kept clean Cleaning should be done with a soft cloth slightly dampened with water or a solution of water and a mild detergent Do not use an excessively damp cloth that liquid can drip into the vacuum degasser 1200 Series VD User Manual 53 6 Maintenance Removing and Refitting the Top Cover Tools required Screwdriver Pozidriv 1 Preparations Switch OFF the vacuum degasser at the main power switch Disconnect the power cable and remote cable Disconnect all solvent tubes from the ports of the vacuum degasser Remove solvent cabinet from the vacuum degasser Remove vacuum degasser from the stack WARNING Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary
16. e instrument has been disconnected from its source of supply Dangerous voltages capable of causing serious personal injury are present in this instrument Use extreme caution when handling testing and adjusting 1200 Series VD User Manual 71 9 Appendix When working with solvents please observe appropriate safety procedures e g goggles safety gloves and protective clothing as described in the material handling and safety data sheet by the solvent vendor especially when toxic or hazardous solvents are used 78 1200 Series VD User Manual Appendix 9 Safety Symbols Table9 Safety Symbols Symbol Description The apparatus is marked with this symbol when the user should refer to the instruction manual in order to protect risk of harm to the operator and to protect the apparatus against damage Indicates dangerous voltages Indicates a protected ground terminal Indicates eye damage may result from directly viewing the light produced by the deuterium lamp used in this product The apparatus is marked with this symbol when hot surfaces are available and the user should not touch it when heated up A WARNING alerts you to situations that could cause physical injury or death gt Do not proceed beyond a warning until you have fully understood and met the indicated conditions CAUTION i eae A CAUTION alerts you to situations that could cause loss of data or damage of equipment gt Do no
17. e appropriate actions 24 Place the vacuum degasser on the bench Ensure the power switch on the front of the vacuum degasser is OFF switch stands out Connect the power cable to the power connector at the rear of the vacuum degasser Connect the interface cable to the vacuum degasser The interface cable remote cable is a one way connection to send a not ready signal from the degasser to the other modules to shut down the whole system after an error condition of the degasser 1200 Series VD User Manual Installing the Vacuum Degasser 3 Installing the Vacuum Degasser NOTE In an Agilent 1200 Series system the individual modules are connected through a CAN cable The Agilent 1200 Series vacuum degasser is an exception The vacuum degasser can be connected via the APG remote connector to the other modules of the stack The AUX output allows to monitor the vacuum pressure in the degasser chamber An Agilent 1200 Series control module can be connected to the CAN bus at any of the modules in the system except for the degasser The Agilent ChemStation can be connected to the system through one GPIB or LAN cable at any of the modules except for the degasser preferably at the detector For more information about connecting the control module or Agilent ChemStation refer to the respective user manual Power socket Fuse holder Figure 5 Rear of the Vacuum Degasser Status indicator Power switch
18. e tubings to the pump inlet before continueing to prime with the pump Priming with the Pump When the pumping system has been turned off for a certain time for example overnight oxygen will rediffuse into the solvent channels between the vacuum degasser and the pump Solvents containing volatile ingredients will slightly lose these if left in the degasser without flow for a prolonged period of time Therefore priming of the vacuum degasser and the pumping system is required before starting an application 1 Open the purge valve of your pump and set flow rate to 5 ml min 2 Flush the vacuum degasser and all tubes with at least 30 ml of solvent 3 Set flow to required value of your application and close the purge valve 4 Pump for approximately 10 minutes before starting your application 5 Repeat step 1 on page 33 through step 4 on page 33 with other channels where needed 1200 Series VD User Manual 33 3 Installing the Vacuum Degasser Transporting the Vacuum Degasser WARNING Solvents leaking out Solvents remaining in the solvent channels may leak out during transport This can possibly cause personal damage gt Drain any remaining solvents from the degassing channels before transporting the micro vacuum degasser 34 Disconnect the solvent tubes from solvent inlets from front panel Disconnect one of the solvent tubes from your pump Connect syringe adapter to solvent tube of this solvent channel Push syri
19. eness and the vacuum degasser has been mechanically and electrically checked If the shipping container or cushioning material is damaged notify the carrier and save the shipping material for the carriers inspection Delivery Checklist Ensure all parts and materials have been delivered with the vacuum degasser The delivery checklist is shown below To aid in parts identification please see Parts and Materials for Maintenance on page 61 Please report missing or damaged parts to your local Agilent Technologies Sales and Service Office Table3 Vacuum Degasser Delivery Checklist Description Quantity Vacuum Degasser 1 Power Cable 1 Remote Cable 1 Auxiliary Cable As ordered Service Manual 1 Accessory Kit Table 4 on 1 page 21 1200 Series VD User Manual Accessory Kit Contents Installing the Vacuum Degasser Table4 Accessory Kit Contents G1322 68705 Description Part Number Quantity Syringe 5062 8534 1 Syringe Adapter 9301 1337 1 Mounting Tool 0100 1710 1 Waste Tube 5062 2463 1 Connecting Tubes labeled A to D G1322 67300 4 1 Reorder Number pack of 10 2 Reorder Number 5 m 1200 Series VD User Manual 21 3 Installing the Vacuum Degasser mizing the Sta ck Configuration Optimizing the Stack Configuration If your vacuum degasser is part of a system you can ensure optimum performance by limiting the configuration of the system stack to the following configuration This configuration
20. er Manual 63 7 Parts and Materials for Maintenance Accessory Kit 64 Table8 Accessory Kit G1322 68705 Item Description Part Number 1 Syringe 5062 8534 2 Syringe adapter 9301 1337 3 Mounting Tool 0100 1710 4 Solvent tubing kit 4 tubes degasser to pump G1322 67300 5 Waste tube 5062 2463 1 Reorder number pack of 10 Reorder number 5m 1200 Series VD User Manual 1200 Series VD User Manual ei Cables Cable Overview 66 Remote Cables 68 Auxiliary Cable 73 Agilent Technologies 65 8 Identifying Cables Cable Overview Never use cables other than the ones supplied by Agilent Technologies to ensure proper functionality and compliance with safety or EMC regulations Type Description Part Number Analog cables 3390 2 3 integrators 01040 60101 3394 6 integrators 35900 60750 Agilent 35900A A D converter 35900 60750 General purpose spade lugs 01046 60105 Remote cables 3390 integrator 01046 60203 3392 3 integrators 01046 60206 3394 integrator 01046 60210 3396A Series I integrator 03394 60600 3396 Series Il 3395A integrator see details in section Remote Cables on page 68 3396 Series Ill 3395B integrator 03396 61010 HP 1050 modules HP 1046A FLD 5061 3378 HP 1046A FLD 5061 3378 Agilent 35900A A D converter 5061 3378 HP 1040 diode array detector 01046 60202 HP 1090 liquid chromatographs 01046 60202 Signal distribution module 01046 60202 BCD cables 3396 integrator 03396 60560 Ge
21. erational conditions large amount of dissolved gas in mobile phase high flow rates the 115 Torr trigger level for operation mode 1 cannot be reached The vacuum degasser automatically turns into operation mode 2 Normal operation mode 2 is a fixed timing mode Every 2 minutes the degasser is turned ON for 30 seconds This assures a pressure level in the range from 115 to 190 Torr The pressure signal measured with the auxiliary cable is in the range between 600 to 800 mV If the vacuum level of normal operation mode two cannot be achieved within 8 minutes the instrument turns into error mode 1200 Series VD User Manual Using the Vacuum Degasser 4 Continuous Mode The continuous mode is activated either by switching SW1 on the main board to 1 removal of cover is required see Removing and Refitting the Top Cover on page 54 for identifying SW1 see Service Manual Repairs Overview of Internal Parts or by connecting pin 1 white cable and pin 3 green cable of the auxiliary cable to each other When turning ON the vacuum degasser the vacuum pump will run continuously This will establish a vacuum level that is below the trigger level 600 mV 115 Torr of the normal operation mode 1 If the vacuum level of normal operation mode 2 cannot be achieved within 8 minutes the instrument turns into error mode When set to continuous mode the life time of the vacuum pump will be significantly reduced When to use Continuous Mode In c
22. hnologies
23. ia a ground socket Any interruption of the protective earth grounding will cause a potential shock hazard that could result in serious personal injury Whenever it is likely that the protection has been impaired the instrument must be made inoperative and be secured against any intended operation Make sure that only fuses with the required rated current and of the specified type normal blow time delay and so on are used for replacement The use of repaired fuses and the short circuiting of fuse holders must be avoided Some adjustments described in the manual are made with power supplied to the instrument and protective covers removed Energy available at many points may if contacted result in personal injury Any adjustment maintenance and repair of the opened instrument under voltage should be avoided whenever possible When inevitable this has to be carried out by a skilled person who is aware of the hazard involved Do not attempt internal service or adjustment unless another person capable of rendering first aid and resuscitation is present Do not replace components with power cable connected Do not operate the instrument in the presence of flammable gases or fumes Operation of any electrical instrument in such an environment constitutes a definite safety hazard Do not install substitute parts or make any unauthorized modification to the instrument Capacitors inside the instrument may still be charged even though th
24. l Depending on the vacuum sensor batch variations this will result in different voltage readings Regardless of the readings of the various stages no adjustment should be performed on the degasser electronics 1200 Series VD User Manual 41 4 42 Using the Vacuum Degasser See service manual troubleshooting and diagnostics for setting up test meter connections to the degasser for reading the pressure sensor output Normal Operation Mode 1 When the degasser is turned ON the vacuum pump runs and is connected to ambient through the solenoid valve The solenoid valve activates about 15 seconds after turning ON the degasser you can hear it click The vacuum pump then begins to pull a vacuum on the vacuum chamber The voltage measurements begin to decrease rapidly The yellow status LED turns off when the vacuum level reaches 190 Torr DC voltage readings around 800mV The normal operation mode vacuum level 115 Torr is achieved when the DC voltage measures approximately 590 to 600 mV After achieving the normal operation mode vacuum level the solenoid valve turns off The vacuum pump continues to run for a few seconds then it turns OFF When the DC voltage measurement rises back to approximately 600 to 610 mV the turn on process begins again If the vacuum level of normal operation mode one cannot be achieved within 8 minutes the instrument turns into normal operation mode 2 Normal Operation Mode 2 Timing Mode Under certain op
25. ly understood and met A WARNING notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indi cated conditions are fully under stood and met 1200 Series VD User Manual Contents Contents Introduction 5 Introduction to the Vacuum Degasser 6 How the Electronics Work 7 Site Requirements and Specifications 11 Site Requirements 12 Physical Specifications 15 Performance Specifications 17 Installing the Vacuum Degasser 19 Unpacking the Vacuum Degasser 20 Optimizing the Stack Configuration 22 Installing the Vacuum Degasser 24 Flow Connections to the Vacuum Degasser 27 Operational Hints for the Vacuum Degasser 31 Transporting the Vacuum Degasser 34 Using the Vacuum Degasser 35 When to Use a Vacuum Degasser 36 Solvent Information 38 Prevent Blocking of Solvent Filters 39 Operation Modes ofthe Vacuum Degasser 41 Troubleshooting and Diagnostics 45 Overview of the Degasser s Indicators 46 Status Indicators 47 Maintenance 49 Introduction to Maintenance and Repair 50 1200 Series VD User Manual Contents 7 Parts and Materials for Maintenance 61 Cover Parts 62 Power and Status Light Pipes 63 Accessory Kit 64 8 Identifying Cables 65 Cable Overview 66 9 Appendix 75 General Safety Information 76 The Waste Electrical an
26. m in the chamber is not within working range above error limit of 190 Torr the amplifier and comparator circuit sends a signal to the vacuum pump driver and the timers of the vacuum pump timer 1 and the solenoid valve timer 2 The vacuum pump is turned on immediately while the solenoid valve closing is delayed by 15 seconds This time delay allows the vacuum pump to start without load before it is connected to the vacuum chamber The status indicator turns yellow when the pump is activated The status lamp is turned off as soon as the vacuum is below the error limit When the vacuum in the chamber reaches its operation mode 1 approximately 115 Torr the amplifier and comparator circuit turns off the solenoid valve and the vacuum pump is turned off by a timer with a delay of 15 seconds As soon as the pressure sensor detects that the limit of the operation mode 1 has been exceeded e g when dissolved gas from the solvent diffused into the vacuum chamber the vacuum pump is started again as described before The pressure signal is available at the auxiliary output It allows to monitor the vacuum system The upper limit of operation mode 1 is 600 mV Values below 600 mV on the pressure output indicate sufficient vacuum in the chamber If the 600 mV are exceeded the vacuum pump will be started to keep the vacuum with in its working limit The amplifier and comparator circuit also activates the timer 3 when the vacuum in the vacuum chamber
27. n the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accor dance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or subcon tract Software is delivered and licensed as Commercial computer software as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer soft ware as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or dis closure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of the U S Government will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June 1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices CAUTION A CAUTION notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly per formed or adhered to could result in damage to the product or loss of important data Do not proceed beyond a CAUTION notice until the indicated condi tions are ful
28. nal Name Digital ground Prepare run Start Shut down Not connected Power on Ready Stop Start request Not connected Active TTL Low Low Low High High Low Low Agilent 1100 1200 to 3396 Series Il 3395A Integrators Use the cable part number 03394 60600 and cut pin 5 on the integrator side Otherwise the integrator prints START not ready 70 1200 Series VD User Manual Identifying Cables 8 Agilent 1100 1200 to 3396 Series Ill 3395B Integrators Connector03396 61010 Pin 33XX Pin Agilent SignalName Active 1100 1200 TTL 9 1 White Digital ground NC 2 Brown Prepare run Low x 3 3 Gray Start Low 2 z NC 4 Blue Shut down Low e o NC 5 Pink Not connected NC 6 Yellow Power on High 14 7 Red Ready High 4 8 Green Stop Low NC 9 Black Start request Low 13 15 Not connected Agilent 1100 1200 to HP 1050 HP 1046A or Agilent 35900 A D Converters Connector5061 3378 Pin HP Pin Agilent SignalName Active 1050 1100 1200 TTL 1 White 1 White Digital ground 2 Brown 2 Brown Prepare run Low 3 Gray 3 Gray Start Low 4 Blue 4 Blue Shut down Low 5 Pink 5 Pink Not connected 6 Yellow 6 Yellow Power on High 7 Red 7 Red Ready High 8 Green 8 Green Stop Low 9 Black 9 Black Start request Low 1200 Series VD User Manual 71 8 Identifying Cables Agilent 1100 1200 to HP 1090
29. neral purpose spade Lugs G1351 81600 Auxiliary Agilent 1100 Series vacuum degasser G1322 61600 66 1200 Series VD User Manual Identifying Cables 8 Type Description Part Number CAN cables Agilent 1100 1200 module to module 0 5m Ig 5181 1516 Agilent 1100 1200 module to module 1m Ig 5181 1519 External Agilent 1100 1200 Series interface board to general G1103 61611 contacts purpose GPIB cable Agilent 1100 1200 module to ChemStation 1 m 10833A Agilent 1100 1200 module to ChemStation 2 m 10833B RS 232 cable Agilent 1100 1200 module to a computer 34398A This kit contains a 9 pin female to 9 pin female Null Modem printer cable and one adapter LAN cable Twisted pair cross over LAN cable shielded 3m long for 5023 0203 point to point connection Twisted pair cross over LAN cable shielded 7m long for 5023 0202 point to point connection 1200 Series VD User Manual 67 68 Identifying Cables Remote Cables One end of these cables provides a Agilent Technologies APG Analytical Products Group remote connector to be connected to Agilent 1100 1200 Series modules The other end depends on the instrument to be connected to Agilent 1100 1200 to 3390 Integrators Connector 01046 60203 Pin 3390 Pin Agilent SignalName Active 1100 1200 TTL 2 1 White Digital ground NC 2 Brown Prepare run Low 7 3 Gray Start Low 2 NC 4 Blue Shut down Low NC 5 Pink Not connected NC 6
30. nge adapter onto syringe ao A Q N Pull syringe plunger to draw solvent out of vacuum degasser and tubing Continue to draw solvent into syringe until the solvent channel is completely empty 6 Repeat step 2 on page 34 through step 5 on page 34 for the remaining solvent channels 1200 Series VD User Manual 1200 Series VD User Manual 4 Using the Vacuum Degasser When to Use a Vacuum Degasser 36 Solvent Information 38 Prevent Blocking of Solvent Filters 39 Operation Modes of the Vacuum Degasser 41 Normal Operation Mode 1 42 Normal Operation Mode 2 Timing Mode 42 Continuous Mode 43 Error Mode 43 RE Agilent Technologies 35 4 Using the Vacuum Degasser When to Use a Vacuum Degasser Unspecified Conditions Operating the instrumentation under conditions other than their intended use might result in a potential safety hazard or might damage the instrumentation gt Never operate your instrumentation under conditions other than specified by the vendor 36 Pumps that mix the solvent on the low pressure side like the Agilent 1200 Series quaternary pump do need degassing and must be equipped with a vacuum degasser or alternative degassing systems for example helium Isocratic pumps and high pressure mixing pumps do not always require degassing However for the following conditions the vacuum degasser is also recommended for an isocratic or a high pressure mixing pump e if your detector is used with maxim
31. nts through filters or membranes that remove algae Exchange solvents every two days or refilter e If the application permits add 0 0001 0 001 M sodium azide to the solvent e Place a layer of argon on top of your solvent e Avoid exposure of the solvent bottles to direct sunlight Checking the Solvent Filters The solvent filters are on the low pressure side of the pumping system A blocked filter therefore does not affect the pressure readings of the pump The pressure readings cannot be used to indetify blocked filters If the solvent cabinet is placed on top of the vacuum degasser the filter condition can be checked in the following way Remove the tubing at the inlet port of the vacuum degasser If the filter is in good condition the solvent will freely drip out of the solvent tube due to hydrostatic pressure If the solvent filter is partly blocked no solvent or only very little solvent will drip out of the solvent tube Cleaning the Solvent Filters e Remove the blocked solvent filter from the bottle head assembly and place it in a beaker with concentrated nitric acid 35 for one hour e Thoroughly flush the filter with bidistilled water remove all nitric acid e Replace the filter 1200 Series VD User Manual 39 4 Using the Vacuum Degasser Prevent Blocking of Solvent Filters Never use the system without solvent filter installed NOTE y 40 1200 Series VD User Manual Using the Vacuum Degasser 4 Operation M
32. odes of the Vacuum Degasser The vacuum degasser allows various operation modes Operation mode 1 and 2 are the normal operation modes of the degasser e In operation mode 1 the vacuum degasser works at 115 Torr e In operation mode 2 the vacuum degasser works in the range between 115 to 190 Torr The continuous mode can be selected for highest degassing efficiency of the degasser In this mode the vacuum level is below 115 Torr e The error mode is activated in case the degasser cannot achieve a vacuum level of 190 Torr 1 Torr 1 33x10 bar Table5 Operation Modes of the Vacuum Degasser Operation Mode 1 Operation Mode 2 Continuous Mode Error Mode Pressure Range Torr 115 115 190 Below 115 Above 190 DC Voltage Readings Lower Limit 590 600 600 800 Below 600 Above 800 mV rough values Upper Limit 600 610 Operation mode Pump switched onand Pump switched on Pump permantly 8 Minutes of off at upper and lower every 2 minutes for 30 running continuous pumping limit during operation seconds before shutdown Status Indicator OFF below 800 mV OFF below 800 mV OFF below 800 mV YELLOW YELLOW above 800 YELLOW above 800 YELLOW above 800 mV mV mV Failure Actions Switch to Operation Switch to Error Mode Switch to Error Mode Shut down module Mode 2 switch status indicator to RED The voltage readings given for the various modes are approximate values All values are set in the factory according to the vacuum leve
33. ontinuous mode the vacuum pump runs continuously thus generating the highest degassing efficiency of the degasser This mode is only recommended for very sensitive applications e g RI detection Error Mode The error level for the vacuum degasser is 190 Torr approximately 800 mV This level cannot be achieved when there is a failure in the degasser for example leaks etc When the error level is exceeded the yellow status indicator lamp is turned on and the vacuum pump runs continuously If the degasser cannot reach one of the normal operation modes within 8 minutes the status indicator turns red and the vacuum pump is turned OFF 1200 Series VD User Manual 43 4 Using the Vacuum Degasser Operation Modes of the Vacuum Degasser 44 1200 Series VD User Manual 1200 Series VD User Manual E and Diagnostics Overview of the Degasser s Indicators 46 Status Indicators 47 Power Supply Lamp 47 Instrument Status Indicator 48 Agilent Technologies 45 5 Troubleshooting and Diagnostics Overview of the Degasser s Indicators Status Indicators The vacuum degasser is provided with two status indicators which indicate the operational state ready busy and error states of the vacuum degasser The status indicators provide a quick visual check of the operation of the vacuum degasser see Overview of the Degasser s Indicators on page 46 Hardware Symptoms A red status lamp at the vacuum degasser indicates a
34. optimizes the system flow path ensuring minimum delay volume from point of solvent mixing to head of column and dead volume from point of injection to detector outlet Solvent Cabinet Degasser Quaternary Pump Autosampler Column Compartment Detector Instant Pilot Figure 3 Recommended Stack Configuration Front View 22 1200 Series VD User Manual Installing the Vacuum Degasser Optimizing the Stack Configuration Remote cable Pressure output to recorder CAN Bus cable to handheld controller AC power CAN Bus cable for inter module communication LAN to Analog signal LC ChemStation to recorder AC power Figure 4 Recommended Stack Configuration Rear View 1200 Series VD User Manual 23 3 3 Installing the Vacuum Degasser Installing the Vacuum Degasser Parts required Preparations Description Vacuum degasser Power cable Interface cable as ordered see Cable Overview on page 66 Locate bench space Provide power connections Unpack the vacuum degasser module CAUTION Defective on arrival problems If there are signs of damage please do not attempt to install the module Inspection by Agilent is required to evaluate if the instrument is in good condition or damaged gt Notify your Agilent sales and service office about the damage gt An Agilent service representative will inspect the instrument at your site and initiat
35. or tube fittings solvents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 1 Remove the front panel 2 Unclip the clips on the top cover In Tre A A EN Ode Clip m S N I Wa z D A J 54 1200 Series VD User Manual Maintenance 6 Introduction to Maintenance and Repair 3 Lift the cover away 4 Unscrew the screws on the top plate and remove the plate by lifting its back and then sliding the plate to the front Screws 5 Place the metal cover onto the housing Lower the front of the plate first then slide panel to the back Assure the four metal tabs of the panel slide underneath the Z plane Fix the two holding screws Metal Tabs Screws 6 Replace the top cover Ensure the clips are seated correctly 1200 Series VD User Manual 55 6 Maintenance troduction to Maintenance and F 7 Reinstall the front cover 8 Reinstall the vacuum degasser in your system stack and connect the cables and capillaries and turn on the vacuum degasser 56 1200 Series VD User Manual Maintenance 6 Assembling the Main Cover When e If cover is broken Parts required Part number Description 1 5065 9989 Cover kit includes base top
36. problem with the vacuum system or with the electronic control The vacuum degasser generates an error output on the remote lines 46 1200 Series VD User Manual Troubleshooting and Diagnostics 5 Status Indicators Two status lamps are located on the front of the vacuum degasser The left lamp indicates the power supply status the right lamp indicates the vacuum degasser status Status indicator Power supply lamp Figure 10 Location of Status Lamps Power Supply Lamp The power supply lamp is integrated into the main power switch When the lamp is illuminated green the power is ON 1200 Series VD User Manual 47 5 Troubleshooting and Diagnostics Instrument Status Indicator CAUTION The instrument status indicator indicates one of three possible instrument conditions When the lamp is OFF the vacuum degasser is in ready condition only if the power supply lamp is ON otherwise the instrument is switched OFF or there is a defect in the power supply A ready condition exists when there is sufficient vacuum in the vacuum degasser A busy condition is indicated when the lamp is yellow A busy condition exists when the vacuum pump of the vacuum degasser is working to generate or maintain vacuum in the chambers This is the case when the vacuum degasser is turned ON for the first time or the pressure rises above its limit during normal operation An error condition is indicated when the lamp is red An error
37. r 3 If the vacuum degasser is not used with an Agilent 1200 Series pump connect the waste tube from the accessory kit to the waste outlet and place into your waste system 4 Put the bottle head assemblies into solvent bottles containing your mobile phase 5 Connect the solvent tubes from the bottle head assemblies to the inlet connectors A to D typically the left connection of the channel of the vacuum degasser Use the mounting tool shown in Figure 8 on page 28 to fix the tube screw Fix the tubes in the clips of the vacuum degasser a D Figure 8 Mounting Tool 28 1200 Series VD User Manual Installing the Vacuum Degasser 3 6 Connect the outlet tubes to the output ports typically right connection of the channel of the vacuum degasser 7 Prime the degasser before first use see Priming the Degasser on page 31 Atmospheric gases can diffuse through the tubing and dissolve in the mobile phase solvents For best chromatographic results keep the length of tubing between the vacuum degasser and your pump as short as possible 1200 Series VD User Manual 29 3 Installing the Vacuum Degasser Flow Connections to the Vacuum Degasser Bottle Head Assembly Solvent cabinet Vacuum Degasser Inlet tube Outlet tube Tube clip Purge valve Pump head Inlet adapter Waste tubing Outlet capillary to sampler MCGV Figure 9 Flow Connections to the Vacuum Degasser with Quarternary Pump 30 1200 Serie
38. r with water If you are changing to a solvent that is immiscible with the solvent currently in the tubing continue as follows Replace the current solvent with iso propanol if current solvent is organic or with water if current solvent is an inorganic buffer or contains salt Disconnect solvent outlet tube of the channel that is supposed to be primed from your pump 4 Connect syringe adapter to solvent outlet tube 5 Push syringe adapter onto syringe Pull syringe plunger to draw at least 30 ml of solvent through degasser and tubing 7 Replace the priming solvent with the new solvent of your choice 8 Pull syringe plunger to draw at least 30 ml of solvent through degasser and tubing Disconnect syringe adapter from solvent tube 10 Connect solvent tube to your pump 11 Repeat step 3 on page 32 to step 10 on page 32 for the other solvent channels 1200 Series VD User Manual Installing the Vacuum Degasser 3 When priming the vacuum degasser with a syringe the solvent is drawn through the degasser tubes very quickly The solvent at the degasser outlet will therefore not be fully degassed Pump for approximately 10 minutes with your selected flow rate before starting any application This will allow the vacuum degasser to properly degas the solvent in the degasser tubes The pump should never be used for priming empty tubings never let the pump run dry Use the syringe to draw enough solvent for completely filling th
39. s VD User Manual Installing the Vacuum Degasser 3 Operational Hints for the Vacuum Degasser Priming the Degasser The vacuum degasser can be primed either by drawing solvent through the degasser with a syringe or by pumping with the connected pump Priming the vacuum degasser with a syringe is recommended when e vacuum degasser is used for the first time or vacuum tubes are empty or e changing to solvents that are immiscible with the solvent currently in the vacuum tubes Priming the vacuum degasser by using the pump at high flow rate 3 5 ml min is recommended when e pumping system was turned off for a length of time for example overnight and if volatile solvent mixtures are used or e if solvents have been changed 1200 Series VD User Manual 31 3 Installing the Vacuum Degasser Priming with a Syringe Before using a new degasser or new tubings for the first time Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary or tube fittings solvents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 32 Prime all tubings with at least 30 ml of iso propanol no matter whether the channels will be used with organic mobile phase o
40. s is below operation mode 1 The timer 3 is reset when operation mode 1 is reached within a maximum of 8 minutes If operation mode 1 is not reached and the time 8 minutes of timer 3 elapsed the timer mode operation mode 2 is activated In this mode the vacuum pump is automatically turned on every 2 minutes for a time frame of 30 seconds Timers 1 and 2 are activated as described earlier The error monitor continuously checks the error limit of the degasser 190 Torr If the error limit is exceeded for example leak in chamber the error timer is activated and the yellow status indicator lamp is turned on The vacuum pump is turned on continuously If the vacuum pump cannot reach either of its two operation modes within 8 minutes error timer limit the error driver is activated The error driver will deactivate the vacuum pump and solenoid valve The status lamp turns red and the error output on the remote connector will be activated 1200 Series VD User Manual Introduction 1 The error output at the remote connector provides a contact closure potential free open collector maximum load 35 V DC 50 mA as long as the error condition is active The error condition is set closed when the status light shows the error condition red The continuous mode overwrites all other operation modes of the degasser When activated switch SW1 on the electronic board or via the auxiliary cable the vacuum pump is forced into continuous mode and is
41. s require opening the main cover of the vacuum degasser gt To prevent personal injury remove the power cable from the vacuum degasser before opening the cover gt Do not connect the power cable to the vacuum degasser while the covers are removed WARNING Toxic and hazardous solvents The handling of solvents and reagents can hold health risks gt When opening capillary or tube fittings solvents may leak out gt Please observe appropriate safety procedures for example goggles safety gloves and protective clothing as described in the material handling and safety data sheet supplied by the solvent vendor especially when toxic or hazardous solvents are used 50 1200 Series VD User Manual Maintenance 6 CAUTION Electronic boards and components are sensitive to electrostatic discharge ESD ESD can damage electronic boards and components gt In order to prevent damage always use ESD protection when handling electronic boards and components CAUTION The sheet metal plates of the degasser are very thin Although they have been deburred they are still quite sharp You may cut your hands or fingers gt Never slide your fingers along the edges of the enclosure 1200 Series VD User Manual 51 6 52 Maintenance Using the ESD Strap Electronic boards are sensitive to electronic discharge ESD In order to prevent damage always use an ESD strap supplied in the standard accessory kit when han
42. steel corrosive solvents Solutions of alkali halides and their respective acids for example lithium iodide potassium chloride and so on High concentrations of inorganic acids like sulfuric acid especially at higher temperatures replace if your chromatography method allows by phosphoric acid or phosphate buffer which are less corrosive against stainless steel Halogenated solvents or mixtures which form radicals and or acids for example 2CHCls Oo 2COCl 2HC1 This reaction in which stainless steel probably acts as a catalyst occurs quickly with dried chloroform if the drying process removes the stabilizing alcohol Chromatographic grade ethers which can contain peroxides for example THF dioxane di isopropylether Such ethers should be filtered through dry aluminium oxide which adsorbs the peroxides Mixtures of carbon tetrachloride with 2 propanol or THF dissolve stainless steel 1200 Series VD User Manual Using the Vacuum Degasser 4 Prevent Blocking of Solvent Filters Contaminated solvents or algae growth in the solvent bottle will reduce the lifetime of the solvent filter and will influence the performance of the pump This is especially true for aqueous solvents or phosphate buffers pH 4 to 7 The following suggestions will prolong lifetime of the solvent filter and will maintain the performance of the pump e Use sterile if possible amber solvent bottles to slow down algae growth e Filter solve
43. switch 1200 Series VD User Manual Maintenance 6 Exchanging the Status Light Pipe When If part is broken Tools required Screwdriver Pozidriv 1 Parts required Part number Description 1 5041 8384 Status light pipe Preparations Remove the front cover and top cover see Removing and Refitting the Top Cover on page 54 1 The status light pipe is clipped into the top cover 2 Replace the top cover see Removing and Refitting the Top Cover on page 54 1200 Series VD User Manual 59 6 Maintenance Introduction to Maintenance and Repair 60 1200 Series VD User Manual 1200 Series VD User Manual 7 Parts and Materials for Maintenance Cover Parts 62 Power and Status Light Pipes 63 Accessory Kit 64 RE Agilent Technologies 61 7 Parts and Materials for Maintenance Cover Parts 62 Table6 Cover Parts Item Description Part Number 1 Set of all plastic covers top side base 5065 9989 2 Front cover 5065 9990 3 Logo plate Agilent 1200 5042 8901 4 Tube clip 5041 8387 Figure 13 Cover Parts 1200 Series VD User Manual Parts and Materials for Maintenance 7 Power and Status Light Pipes Table 7 Power and Status Light Pipes Item Description Part Number 1 Power switch coupler 5041 8383 2 Light pipe power switch 5041 8382 3 Power switch button 5041 8381 4 Light pipe status lamp 5041 8384 Figure 14_ Power and Status Light Pipes 1200 Series VD Us
44. t proceed beyond a caution until you have fully understood and met the indicated conditions 1200 Series VD User Manual 79 9 Appendix The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC Abstract The Waste Electrical and Electronic Equipment WEEE Directive 2002 96 EC adopted by EU Commission on 13 February 2003 is introducing producer responsibility on all Electric and Electronic appliances from 13 August 2005 s This product complies with the WEEE Directive 2002 96 EC marking requirements The affixed label indicates that you must not discard this electrical electronic product in domestic household waste Product Category With reference to the equipment types in the WEEE Directive Annex I this product is classed as a Monitoring and Control instrumentation product Do not dispose off in domestic household waste To return unwanted products contact your local Agilent office or see www agilent com for more information 80 1200 Series VD User Manual Appendix 9 Radio Interference Cables supplied by Agilent Technoligies are screened to provide opitimized protection against radio interference All cables are in compliance with safety or EMC regulations Test and Measurement If test and measurement equipment is operated with unscreened cables or used for measurements on open set ups the user has to assure that under operating conditions the radio interference limits are still me
45. t within the premises 1200 Series VD User Manual 81 9 Appendix Sound Emission Manufacturer s Declaration This statement is provided to comply with the requirements of the German Sound Emission Directive of 18 January 1991 This product has a sound pressure emission at the operator position lt 70 dB Sound Pressure Lp lt 70 dB A At Operator Position e Normal Operation e According to ISO 7779 1988 EN 27779 1991 Type Test 82 1200 Series VD User Manual Appendix 9 Agilent Technologies on Internet For the latest information on products and services visit our worldwide web site on the Internet at http www agilent com Select Products Chemical Analysis It will provide also the latest firmware of the Agilent 1200 Series modules for download 1200 Series VD User Manual 83 Index Index A accessorykit 21 64 Agilent on internet 83 algae growth 39 ambient non operating temperature 15 ambient operating temperature 15 analog output 17 17 analog cable 66 APG remote connector 25 AUX output 25 auxiliary cable 66 73 B BCD cable 66 bench space 14 bottle head assembly 27 bottle 27 busy condition 48 C cable analog 66 auxiliary 20 66 73 BCD 66 CAN 25 external contacts 67 GPIB 25 67 interface 24 LAN 67 power 20 54 84 remote 20 66 68 RS 232 67 cables overview 66 CAN cable 25 changing solvents 32 ChemStation 25 cleaning the instrument 53 condensation 14
46. te Electrical and Electronic Equipment WEEE Directive 2002 96 EC 80 Radio Interference 81 Sound Emission 82 Agilent Technologies on Internet 83 RE Agilent Technologies 75 9 Appendix General Safety Information General Safety Information The following general safety precautions must be observed during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument Agilent Technologies assumes no liability for the customer s failure to comply with these requirements WARNING Ensure the proper usage of the equipment The protection provided by the equipment may be impaired gt The operator of this instrument is advised to use the equipment in a manner as specified in this manual General This is a Safety Class I instrument provided with terminal for protective earthing and has been manufactured and tested according to international safety standards 76 1200 Series VD User Manual Appendix 9 Operation Before applying power comply with the installation section Additionally the following must be observed Do not remove instrument covers when operating Before the instrument is switched on all protective earth terminals extension cords auto transformers and devices connected to it must be connected to a protective earth v
47. turned on as long as the degasser is switched ON 1200 Series VD User Manual 9 1 Introduction How the Electronics Work 10 1200 Series VD User Manual 1200 Series VD User Manual 2 Site Requirements and Specifications Site Requirements 12 Power Considerations 12 Power Cords 13 Bench Space 14 Environment 14 Physical Specifications 15 Performance Specifications 17 BE Agilent Technologies 2 Site Requirements and Specifications Site Requirements A suitable environment is important to ensure optimum performance of the instrument Power Considerations The vacuum degasser power supply has wide ranging capability see Table 1 on page 15 It accepts any line voltage in the range mentioned below Consequently there is no voltage selector in the rear of the vacuum degasser There are two externally accessible fuses that protect the power supply Incorrect line voltage at the instrument Shock hazard or damage of your instrumentation can result if the devices are connected to a line voltage higher than specified gt Connect your vacuum degasser to the specified line voltage CAUTION Provide access to the power plug In case of an emergency it must be possible to disconnect the instrument from the power line at any time gt Make sure the power connector of the instrument can be easily reached and unplugged gt Provide sufficient space behind the power socket of the instrument to unplug the cable
48. um sensitivity in the low UV wavelength range e if your application requires optimum injection precision or e if your application requires highest retention time reproducibility mandatory at flow rates below 0 5 ml min e if your sample or detection is sensitive to dissolved oxygen in the mobile phase degradation Generally a degasser should be used when negative effects due to dissolved gas in the mobile phase exceed the limits that are acceptable for the user Negative effects that can be caused by dissolved gas are e Unstable flow due to unstable pumping conditions This may result in a high ripple unstable pressure at constant flow and with constant mobile phase composition or high standard deviations of peak retention times and peak areas especially at low flow rates e Baseline noise on detectors that are sensitive to changes in the refractive index e g RI detector or UV detector in the low UV range both at maximum sensitivity e Sample degradation 1200 Series VD User Manual Using the Vacuum Degasser 4 When to Use Vacuum Dega e Fluorescence Quenching due to dissolved oxygen Baseline drift in electrochemical detectors due to dissolved oxygen especially in reduction mode 1200 Series VD User Manual 37 4 Using the Vacuum Degasser Solvent Information 38 Always filter solvents through 0 4 um filters small particles can permanently block the capillaries Avoid the use of the following

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