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The Advanced Micro-Osmometer Model 3300

1. Electronic circuits inside The main circuitry is contained on two printed circuit boards in a moth erboard daughterboard configuration More in depth technical details are available in the unit service manual sold separately Processor Board The processor board is the smaller of the two printed circuit boards and contains the Intel 80C186EB central processor two flash EPROM s RAM Realtime clock watchdog circuit and glue logic Instrument Operation Application Board The application board contains the circuits for con trolling and interfacing with the other subsystems such as the keypad display cooling assembly RS 232 printer and barcode ports etc The application board also contains two 20 bit A D converters for reading the sample and block probe thermistors Sampler Figure 1 The sampler is a specialized pipette which contains and positions a very specific volume of sample for each test The sampler consists of the pipette body and disposable plastic sample tip that must be replaced after each test Operating cradle Figure 1 The operating cradle is a sliding mechanism that guides and introduces the sampler to the freezing chamber Cooling chamber amp measurement and control circuits A high reliability thermoelectric module controls cooling chamber tem perature while a thermistor sample probe measures the dynamic tem perature of a sample Test results based on the freezing point of the sample are automa
2. Recalibration This message indicates that you need to re Needed calibrate your instrument Re calibrate close ly following the instructions in Chapter 3 If the error message repeats after successful re calibration obtain Hot Line Service Reset Probe Config Most likely this message indicates that you should reset your block and sample probe bin numbers as described in Chapter 4 If this does not solve the problem contact Advanced Instruments for Hot Line Service Unless you are running a sample of distilled Result 0 Bad water or some other fluid with an osmolality Calibration of zero you should not see this message If you were expecting a sample to test above zero then you may need to re calibrate your instrument Advanced Osmometer Model 3300 User s Guide 40 ROM Serial Number Error Try restarting your instrument If this does not solve your problem contact Advanced Instruments for Hot Line Service Sample Freeze Error This message can be displayed for a number of reasons Check your technique and the condition of your sampler If neither of these is the source of the problem you should check your sample and block probe numbers If this does not solve your problem contact Advanced Instruments for Hot Line Service Sample Pre freeze Sample Probe Failure A sample pre freeze message usually appears as a result of an unclean freezin
3. e Do not operate this product with the covers removed or unsecured xi Advanced Osmometer Model 3300 User s Guide Notes xii E Foreword Principles of Freezing Point Osmometry When a solute is dissolved in a pure solvent the following changes in the solution s properties occur e the freezing point is depressed e boiling point is raised e osmotic pressure is increased and e vapor pressure is lowered These are the so called colligative or concentrative proper ties of the solution which within reasonable limits change in direct proportion to the solute concentration in other words the number of particles in solution Of the colligative properties measurement of the freezing point allows the concentration of an aqueous solution to be easily determined with great precision The freezing point of pure H O is precisely 0 010 C One mole of a non dissociating solute such as glucose where the solute does not dissociate into ionic species but remains intact when dissolved in 1 kilogram kg of water will depress the freezing point by 1 858 C This change is known as the freezing point depression constant for water The freezing point depression also depends upon the degree of dis xiii Advanced Osmometer Model 3300 User s Guide Xiv sociation of the solute Ifthe solute is ionic the freezing point is depressed by 1 858 C for each ionic species For example if one mole of sod
4. Step 2 Unpack your instrument 1 Step 3 Obtain additional items 2 Step 4 Power up your instrument 2 Step 5 Set your date and time 3 Step 6 Check your initial calibration 3 Step 7 Proceed to instrument operation 4 Chapter 2 Instrument Operation 5 Hazardous material cautions 5 Function of major components 5 Sample handling 10 Standards amp controls 10 Sample test procedure 11 Sample test errors 12 Recall results 13 Repeatability tips 13 Statistics 14 Changing operating settings 15 iii Advanced Osmometer Model 3300 User s Guide iv Using a barcode scanner with the Model 3300 Using a printer with your the Model 3300 Using the Model 3300 s RS 232 port Instrument software update Chapter 3 Calibration Calibration procedure Calibration notes Chapter 4 Troubleshooting amp Service Service amp maintainance cautions Obtaining service Troubleshooting checks Internal diagnostics Fuse replacement Chamber cleaning Sample plunger replacement and calibration Appendices Appendix A Troubleshooting Table Appendix B Product Specifications Appendix C Regulatory Notices Appendix D Warranty and warranty duties Appendix E Supplemental RS 232 information Appendix F Service log Index 17 18 18 19 21 21 22 25 25 27 28 28 33 34 34 37 43 45 47 51 53 55 Mi Parts amp Accessories To order parts and accessories contact the Advanced Instruments Cust
5. compatible computer program for use in plot ting freezing curves In Standard mode ony the final result and some status mes sages will be presented to the serial port At Osmometer Ready the two multifunction soft keys are labeled CALIB and DIAG respectively Press the soft key labeled DIAG to enter the diagnostic test menu While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX soft key to cycle through your choices until you see 12 Data Capture Press START to enter the selection menu Select the soft key for the mode you want to use Press the two soft keys simultaneously to lock in your selection Press the two soft keys again simultaneously to return to Osmometer Ready Language To reset the display language take the following steps l At Osmometer Ready the two multifunction soft keys are labeled CALIB and DIAG respectively Press the soft key labeled DIAG to enter the diagnostic test menu While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX keypad to cycle through your choices until you see 14 Select Language 17 Advanced Osmometer Model 3300 User s Guide 3 Press START to enter the Select Language mode Your instrument will display the currently set language 4 Press the left soft key to switch betwee
6. dissociate C molal concentration of the solution e Molarity Moles of solute per liter of solution e Osmolarity Osmols of solute particles per liter of solution Although molarity and osmolarity may be common units of xvi Foreword measurement in other branches of chemistry they are not used in osmometry because the ratio of solute to solution is not linear Molality and osmolality are linear independent of the effect of temperature and volume displaced by solute A calculated con version between units of molality and molarity is complex and generally unnecessary when the terms are properly understood Freezing Point Melting Point The temperature at which the liquid and solid phases of a substance will remain together in equilibrium Freezing Point Depression When a solute is added to a solvent the freezing point of the solvent is lowered In aqueous solutions one mOsm of solute per kilogram of water depresses the freezing point by 1 858 millidegrees Celsius m C Supercooling The tendency of a substance to remain in the liquid state when cooled below its freezing point Crystallization Temperature Aqueous solutions can be induced to freeze i e crystallize most reliably when supercooled When super cooled agitating the solution freeze pulse induces crystal formation The crystallization temperature is the temperature at which crystalliza tion is induced During crystallization the heat of fusion rais
7. power has been restored even if power restoration is immediate Step 5 Set your date and time If you wish you may now change your date and time settings by using the instructions found on page 13 of this user s guide Step 6 Check your initial calibration Your instrument has been carefully calibrated by the manufacturer but to verify that this calibration is accurate within your operating environ ment run tests on the 290 mOsm kg reference solution and or Protinol biological controls before testing samples The number and type of tests that you run should be determined by your own laboratory s standard protocol Use the operating technique described in Chapter 2 when run ning these controls If you determine that the initial calibration is incorrect re calibrate as described in Chapter 3 NOTE If your Model 3300 has just been moved from a different loca tion it should be allowed to warm up for 20 to 30 minutes before you run calibration verification tests Advanced Osmometer Model 3300 User s Guide Step 7 Proceed to instrument operation If you have followed the steps outlined in this chapter your instrument is ready for use To learn how to operate your instrument read the next chapter Instrument Operation We strongly recommend that you read the entire second chapter before attempting to operate your osmometer FA Instrument Operation A A In order to run your instrument properly i
8. reason or if an alter nate cord is used the cord must be approved for use in the local country The power cord must be approved for the product s listed operating voltage and be rated at least 20 greater than the ampere ratings marked on the product s electrical ratings label The cord end that connects to the product must have an IEC 60320 connector Plug the product into an approved grounded electrical outlet Do not disable the power cord s grounding plug If an extension cord or power strip is used make sure that the cord or strip is rated for the product and that the total ampere ratings of all products plugged into the extension cord or strip do not exceed 80 of the cord s or strip s rating limit Route power cords so that they will not be walked on tripped on or pinched by items placed upon or against them Pay particular atten tion to the plug electrical outlet and the point where the cord exits the product Do not pull on cords and cables When unplugging cords or cables grasp the corresponding connector Safe Use e Do not install or use this product in any area subject to extreme short term temperature variations or locations that exceed the speci fied operating environment temperatures e Never use this product in a wet area e To avoid injury or fire hazard do not operate this product in an explosive atmosphere e Do not install or use the product on an unstable non level work sur face
9. the instrument display shows Calibration Not Complete after six samples at either calibration level the calibration procedure has failed and you should carefully repeat the procedure If you experience Calibration Not Complete more than once you have a repeatability problem and should consult Chapter 4 for troubleshooting and service information Calibration notes The Model 3300 will retain its previous calibration data until it com pletes a re calibration and the display reads Calibration Complete If the instrument has calibration information in memory and you have not changed any probes the first test result displayed should be close to the nominal value of the standard loaded If the instrument has no calibration information in memory or a probe has been changed the test result displayed may be far from the nominal value of the standard loaded If the displayed values repeat consistently the calibration will automatically adjust when the calibration test sequence is complete Calibration You may terminate the calibration procedure at any time by raising the operating cradle and pressing CANCEL The Model 3300 will display Test Canceled beep once then display Calib Canceled and beep again The existing calibration will not be affected If you accidentally press a function soft key during a series of regu lar tests the same procedure will terminate the calibrate mode with out affecting the cal
10. times in both a clockwise and counterclockwise direc tion Withdraw chamber cleaner and insert the opposite end Rotate the chamber cleaner in the same manner and leave it in the sample port until your next test 11 Remove the used sample tip from the sampler by pressing firmly enough on the sampler plunger to dislodge the tip Discard the used sample tip 12 Blot the Teflon plunger tip with a soft no lint non ionic paper tis sue Be careful not to dislodge the tip 13 To run additional tests repeat this procedure beginning with step 1 Sample test errors Occasionally a test will not run to completion and your instrument will display an error message Refer to the Troubleshooting Table appendix at the end of this user s guide for an explanation of a particular mes sage Instrument Operation Repeatability Tips Treat all samples as well as standards and reference solutions uni formly before the test Microsamples are more susceptible to contamination and evapora tion than larger samples Avoid leaving sample containers open Cold samples are susceptible to condensation warmer samples are susceptible to evaporation Use only the Advanced Model 3300 sampling system Each sys tem comes with specific operating instructions and re order infor mation If an occasional sample produces irregular results discard obvious ly discrepant readings as long as the instrument has been producing accurate readings repeatedly Re
11. to Osmometer Ready again press the two function key pads simultaneously Changing operating settings Date and time To reset the date and time take the following steps l At Osmometer Ready the two multifunction soft keys are labeled CALIB and DIAG respectively Press the soft key labeled DIAG to enter the diagnostic test menu While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX soft key to cycle through your choices until you see 13 Set Date Time Press START to enter the Set Date Time mode Your instrument will display the current date and time Press the left soft key to select the date or the right soft key to select the time If you choose to set the date a Select between DD MM YYYY and MM DD YYYY formats Your instrument will display the current date in the format you selected and a blinking cursor element b Use the left soft key to increment the number at the cursor and the right soft key to change between digits in the date until the correct date is displayed 15 Advanced Osmometer Model 3300 User s Guide 16 c Press both soft key buttons to save the new date setting and return to the diagnostic test menu If you choose to set the time a Select between AM PM and 24 hour formats After you select a display format your instrument will display the current time in the selected format b U
12. to the factory Advanced Instruments cannot accept collect shipments without prior factory approval Please insure the shipment or accept the damage risk 27 Advanced Osmometer Model 3300 User s Guide 28 Troubleshooting checks Check operational requirements If you are experiencing difficulties with your instrument first carefully review the operational requirements listed in the product specifications and the recommended setup and operating procedures Check fuses You will find the power switch and fuse holder beside the power cord connector on the back panel of the instrument Switch the power switch to the off position and disconnect the power cord Use a small flat bladed screwdriver or similar tool to pry out the fuse holder Visually check for a blown fuse If there is any doubt test the fuses with a continuity checker or ohmmeter or simply replace them with new fuses Check error messages The software of your instrument is designed in such a way that any incomplete task will be associated with an error message many self explanatory that will help you discover the source of your problem You can find all error messages and descriptions of what they mean in Appendix B Internal diagnostics The diagnostic menu allows any of a series of tests to be run to check one functional subsystem of the instrument or to perform some neces sary adjustment or set up At Osmometer Ready the function keypads are respectiv
13. 2 Information The DB 9 RS 232 port on your instrument conforms to the DTE EIA 232 standard and can reliably communicate over shielded cable up to 10 meters in length depending on the baud rate you use Almost every item of information displayed by your instrument is also transmitted over the RS 232 port including test results all error messages and most display data from the diagnostic menu Data is transmitted asynchronously as start bit 8 data bits and 1 stop bit with no parity Each message transmitted from the communication port is terminated by the sequence Carriage Return OD Hex Line Feed OA Hex Note that your instrument is only capable of outputting information At this time there is no protocol for talking to the instru ment Sample RS 232 Setup As a typical example of a communications program setup the following instructions will describe the process necessary for using your instru ment in conjunction with Hyperterminal for Windows 95 and a null modem cable available from Advanced Instruments as shown in the diagram below You can use this procedure to be sure that your instru ment and cable are operating correctly 1 Create a Hyperterminal connection using the Hyperterminal pro gram 2 While working within that connection choose the Properties selec tion from the File menu 51 Advanced Osmometer Model 3300 User s Guide 3 Select the Configure button 4 Choose the following settings f
14. D Warranty amp Warranty Duties LIMITED MODEL NUMBER WARRANTY SERIAL NUMBER CONDITIONS INSTALLATION DATE Advanced Instruments warrants that for 12 months from WARRANTIES REPRESENTATIONS AND CONDI date of shipment each new product sold or manufactured is TIONS OF ANY KIND EXPRESSED OR IMPLIED IN free from defects in material and workmanship under FACT OR BY LAW IN NO EVENT SHALL AD normal installation use and service except glass plastic and VANCED INSTRUMENTS BE LIABLE UNDER THIS parts warranted by their makers WARRANTY OR OTHERWISE IN ANY MATTER FOR Advanced Instruments agrees that such defects will be ANY REMOTE OR CONSEQUENTIAL DAMAGES RE remedied or new parts furnished in exchange for any SULTING FROM THE BREACH OF THIS WARRANTY defective part delivered by the owner transportation OR CONDITION IMPLIED BY LAW OR OTHERWISE charges prepaid to the factory for our examination If in OR FOR ANY DAMAGE TO THE INSTRUMENT OR our judgment the part contains any such defect it will be ANY PART THEREOF CAUSED BY IMPROPER replaced charging only for transportation INSTALLATION UNAUTHORIZED REPAIR OR AL This warranty does not extend to any instrument sub TERATION NEGLIGENCE ABUSE OR MISUSE IN jected to negligence accident abuse misuse or other im CLUDING USE OF IMPROPER GLASSWARE OR proper operation or which has been tampered with altered REAGENTS ACCIDENT OR ACT OF GOD or repaired by anyone other than Ad
15. Service Results not repeatable too scattered Most often poor repeatability comes as a result of poor technique or not following procedure Be sure all sample tips are clean as well as the sampler itself If the sampler plunger wire has not been changed in 500 tests change it as described in Chapter 4 If the sampler is not properly calibrated re calibrate it using the procedure described in Chapter 4 Block Probe Failure This message indicates a problem with your block probe Contact Advanced Instruments for Hot Line Service 37 Advanced Osmometer Model 3300 User s Guide 38 Block Probe Open This message indicates a problem with your block probe Check the block probe bin number and change if necessary If this does not solve your problem contact Advanced Instruments for Hot Line Service Calibration out of Range Repeat Calib This message indicates that your calibration could not be accepted because the results were not consistent enough Retry the cali bration paying particular attention to tech nique Cooling System Error Event Record Lost This message indicates that your cooling system is not functioning properly Contact Advanced Instruments for Hot Line Service This message indicates that the event record stored in memory has been corrupted Try restarting your instrument If this does not solve your problem Contact Advanced Instruments
16. The Advanced Micro Osmometer Model 3300 User s Guide Gl ADVANCED INSTRUMENTS inc Two Technology Way 781 320 9000 Norwood Massachusetts 02062 USA 800 225 4034 Fax 781 320 8181 www aicompanies com 3305 Rev9 012303 Advanced Osmometer Model 3300 User s Guide ii Copyright This user s guide is copyrighted by Advanced Instruments Inc with all rights reserved Under copyright laws this guide may not be reproduced in any form in whole or part without the prior written consent of Advanced Instruments 2002 Advanced Instruments Inc Trademarks claimed in this document are the property of Advanced Instruments Inc Advanced Instruments has reviewed this guide thoroughly All material contained within is believed reliable but the accuracy and completeness are not guaranteed or warranted and are not intended to be representations or warranties concerning the product described Hot Line Service If you have any questions or problems regarding the proper operation of your instrument please contact our Hot Line Service department e 800 225 4034 toll free within the USA and Canada e US 781 320 9000 elsewhere e 781 320 0811 fax Mi Table of Contents Parts amp Accessories v Calibrators amp Standards vii Safe Use ix Symbol conventions ix General cautions x Foreword Theory and Technique xiii Chapter 1 Installation amp Setup 1 Step 1 Find a location for your instrument 1
17. ady to use a Gets i Calibration For verification that the wire Set Screw is calibrated correctly use Access the following procedure 1 Place a new sample tip on i the sampler tL A T 2 Unscrew the calibration gauge and key 3MO 828 Sampler Plunger 4 Insert the key end of the n E i calibration gauge into the Tip 4 sample tip 5 Visually inspect the posi tion of the end of the sampler plunger tip and the end of the calibration Pompe key There should be no gap between the two Key 6 If necessary reset the sampler plunger wire as i t Banke above Figure 5 Sample plunger replacemen and calibration 35 Advanced Osmometer Model 3300 User s Guide Notes 36 Appendix A Troubleshooting Table Abrupt loss of power A If you lose power to your instrument we recommend that you check that your outlet is providing the correct amount of power Check that your cord is firmly plugged into both the instrument and the outlet Visually inspect the fuses and change as necessary No response when sampler is lowered into operating position If there is no response when you lower the operating cradle into operating position there could be a problem with the internal switch that initiates the test Try turning the instrument on and then off If the problem persists please consult an Advanced Instruments Model 3300 Service Manual or contact Advanced Instruments for Hot Line
18. e Solenoid Driver This message indicates a problem with your Failure solenoid impactor Try restarting your instrument If this does not solve your prob lem contact Advanced Instruments for Hot Line Service Standards Reversed This message will appear during the cali Please Repeat bration procedure if the instrument detects that the low and high calibration standards may have been mixed up and were entered in the wrong order Retry the calibration being sure that the standards are correct System Error Reqs Each of these error messages indicate a differ System Error Prim ent system error Try restarting your instru System Error Comm ment If this does not solve your problem con System Error Unkn tact Advanced Instruments for Hot Line System Error Trap Service 41 Advanced Osmometer Model 3300 User s Guide 42 T E Driver Failure This error message indicates a problem with your thermoelectrics Try restarting your instrument If this does not solve your prob lem contact Advanced Instruments for Hot Line Service Test Time out Error This message indicates that your instrument was unable to complete the test in the allot ted time Be sure that your operator tech nique is sound and retry the test If the problem persists check your block probe number If you need more assistance call for Hot Line Service Appendi
19. ely labeled CALIB and DIAG with the supervisor operator keyswitch in the Supervisor position 1 If necessary press both soft keys simultaneously to display DIAG 2 When DIAG is displayed press the function button under DIAG When you press the DIAG soft key 1 Recall Results and the choice of START or INDEX is displayed 3 While any diagnostic menu item is being displayed use either the Troubleshooting amp Service START soft key to start the displayed test the INDEX soft key to display the next menu item or both soft keys simultaneously to exit from the menu 4 If you choose to press the START soft key execution of the cho sen function may require the use of either of the soft keys for further selections as designated below To end any function and return to the menu press both soft keys and release them simultaneously 5 You may then use INDEX to index to another item on the menu or press both soft keys and release them simultaneously to exit from the diagnostic menu to Osmometer Ready Any instructions necessary for the menu function started are provided on the display A description of each menu item follows A D Test This set of tests may be used to test the block probe the sample cooling assembly or the sample probe On entry the A D tests display the target sample cooling block tempera ture the channel being read and the current channel reading or duty cycle The dis
20. ents agree to the following responsibilities which constitute contractual warranties and conditions between the seller and the user for the maximum benefit and useful ness of the instrument AI1YW REV O ADVANCED INSTRUMENTS WARRANTS THAT IT 1 Has produced equipment equal to or exceeding that of any competitive product in the same price range in standards of design material and workmanship 47 Advanced Osmometer Model 3300 User s Guide Knows of no defects in design or mate rials which may cause bodily injury Will endeavor to advise the user of changes or improvements in the instru ment as they are developed so that the user may take steps to improve the safety and performance of his equip ment throughout its useful life Will replace or repair equipment according to the guarantee on the attached warranty Will cooperate closely in common defense of any accident involving this equipment or third party suit against the user or operating personnel if advised immediately by the user of the occurrence of any accident THE USER WARRANTS THAT 1 The instrument will be used reason ably The instrument will be regularly main tained according to this manual including a log of all service tests and repairs performed on the equipment and records will be kept of all requests for repair made to Advanced Instruments where such repairs were beyond the ability of local service per sonnel T
21. es the tem perature of the sample to an ice water freezing point plateau Heat of Fusion The heat released when the mobile molecules of a liq uid are frozen into rigid ice crystals Freezing Point Plateau The constant temperature maintained during the time that ice and liquid exist in isothermal equilibrium after crystal lization occurs Advanced Osmometer Model 3300 User s Guide Notes xviii Ed Installation amp Setup In order to set up your instrument properly it is important that AN you read and follow the steps in this section Please follow these steps carefully and be sure to read Chapter 2 Operating Your Osmometer before attempting to run tests on your instrument Step 1 Find a location for your instrument When choosing a location for your new osmometer be sure to meet the following criteria e Adequate space The dimensions of the instrument are 13 x 10 5 x 15 5 inches 26 7 x 33 x 39 4 cm Be sure to keep your workplace free of debris especially near the front of the instrument where proper ventilation is needed e Electric outlet availability Your instrument will need to AN operate within five feet of a properly grounded three prong electrical outlet capable of continuously supplying 1 ampere at 200 250V to 1 25 amperes at 100 130V If the instrument is not grounded properly its operation may be impaired and a safety hazard may exist Therefore be sure to test the outlet and rec
22. for voltages between 100VAC and 250VAC but appropriately rated fuses must be installed Use 5 x 20mm 250V time delay Type T fuses For 100 130V operation use 1 25 Amp fuses for 200 250V operation use 1 Amp fuses Re install the fuse holder into the back of the instrument Re connect the power cord and switch the power switch to the on position The instrument should start up as normal Chamber cleaning The cooling chamber and probe are easy to keep clean and dry by faith fully following the operating instructions for cleaning the freezing chamber after each test If traces of standards or biological samples are left in the sample chamber however the task will be more difficult and damp cleaning will probably be required Two indicators that damp cleaning may be required are The instrument has been in use but no clean dry chamber cleaner is found in the sample port and successive results on aliquots of the same sample indicate chamber contamination the first aliquot read ing very high and subsequent readings progressively lower FUSE HOLDER Figure 4 Fuse Replacement 33 Advanced Osmometer Model 3300 User s Guide 34 Sample Pre freeze errors begin to occur quite frequently When indicated the cooling chamber may be damp cleaned as follows 1 Dampen do not saturate the end of a chamber cleaner with 70 iso propanol solution 2 Firmly insert the dampened chamber cleaner all the way in
23. fore contacting Advanced Instruments for Hot Line Service be sure to read through this user s guide for instructions on routine adjustments instrument care and troubleshooting If this information does not solve your problem call the appropriate number below e 800 225 4034 toll free within the USA and Canada e US 781 320 9000 elsewhere e 781 320 0811 fax If you purchased your instrument outside of the U S or Canada please contact your Advanced Instruments authorized dealer for service or repair When contacting our service personnel please have the model and seri al numbers from the label on the back of your instrument your user s guide or service manual and the symptoms of your problem ready You should use a telephone as close to your instrument as possible to facili tate making recommended diagnostic checks If you need to order parts or service a purchase order from your purchasing agent will be neces sary After Hot Line diagnosis your service technician may assist you in making minor repairs over the phone dispatch a local service represen tative or have you ship your instrument for factory repair If you need to return an instrument for repair or replacement e Notify our service department to obtain an RMA e Be sure to telephone Advanced Instruments before shipping to avoid any delays e Carefully pack and send everything except your instrument s supply items e Be sure to prepay for any shipment
24. g chamber Clean your chamber as described in Chapter 4 and be sure that the chamber is cleaned after each test If this does not solve your problem you should check the probe bin number and obtain service if nec essary Switch the instrument off then on Check the sample probe by running the A D tests If the error message does not persist and other error messages are not displayed ignore this message Otherwise contact Advanced Instruments for Hot Line Service Sample Probe Open Switch the instrument off then on Check the sample probe by running the A D tests If the error message does not persist and other error messages are not displayed ignore this message Otherwise contact Advanced Instruments for Hot Line Service Troubleshooting Table Set Probe Bin s Your instrument will not function unless there are bin numbers entered for both the block and sample probes To set these num bers see instructions in Chapter 4 Sample Did Not If you heard an impact while the sample Freeze was attempting to run the fault most likely lies with the sample preparation or the block probe Run another test paying close attention to technique If your problem is not resolved check and correct if necessary the block and probe bin numbers If you heard no impact the problem most likely lies with the solenoid impactor Retry the test and if necessary contact Advanced Instruments for Hot Line Servic
25. h the freezing cycle and shows the action of the instrument at each stage of the cycle FAST COOL SAMPLE FREEZING PLATEAU READOUT POINT TEMPERATURE FREEZE TIME THERMODYNAMICS OF SAMPLE Figure 1 Standard Freezing Curve XV Advanced Osmometer Model 3300 User s Guide Definitions Solution A homogeneous mixture of solute and solvent in which the solvent is usually the major component and the solute is the minor one Concentration The ratio of solute to a given amount of solvent molal or ratio of solute to solution molar The amount of solute is usually expressed in terms of moles i e gram molecular weights One mole 6 028 x 10 molecules Avogadro s number One mole of glucose 180 2 g and one mole of sodium chlo ride 58 4 g each contain Avogadro s number of molecules Common units of concentration are e Molality Moles of solute per kilogram of pure solvent e Osmolality Osmols of solute particles per kilogram of pure sol vent As noted above most ionic solutes do not completely dis sociate Osmolality is a unit of concentration that takes into account the dissociative effect Osmolality is usually expressed in mOsm kg H O One milliosmol mOsm is 10 osmols Osmolality is defined as osmol kg H O Osmolality nC where osmotic coefficient which accounts for the degree of mole cular dissociation n number of particles into which a molecule can
26. h precision thermometers to sense the sample temperature to control the degree of supercooling and freeze induction and to measure the freezing point of the sample They can routinely determine differences of 1 mOsm kg H O Foreword Freezing Point Thermodynamics The quickest and most precise way to measure the freezing point of a solution is to supercool it several degrees below its freezing point It is unstable in this state and a mechanical agitation induces crystallization The heat of fusion suddenly liberated causes the sample temperature to rise toward a plateau temperature where a liquid solid equilibrium occurs The equilibrium temperature is by definition the freezing point of the solution Managing the plateau temperature for precise measure ment is the basis for several patents issued to Augustus Fiske The time over which liquid solid equilibrium develops and is main tained is a function of the speed with which the heat of fusion is liber ated vs the speed it is transferred away or absorbed by the surrounding environment This ratio can be slowed and the equilibrium time stretched to give a distinct plateau height measurable to 0 001 C Sensitive thermistor probes monitor the sample temperature and control the thermoelectric cooling element Microprocessor control and auto mated operation minimize imprecision due to operator technique Figure illustrates the temperature of a sample as it progresses throug
27. he instrument will not be altered without written approval from Advanced Instruments Advanced Instruments will be notified immediately if any injury occurs in any association with the instrument and will be allowed prompt and thorough exami nation of the instrument in question 5 Advanced Instruments will be held harmless in cases of injury arising see definitions below a Beyond the useful life of the equipment b From unreasonable use c When Advanced Instruments is not immediately notified of said injury d From interpretation of results DEFINITIONS 1 Useful life is a The same as the depreciation life in the Internal Revenue Service guide lines whether or not the user actual ly depreciates the instrument but not to exceed 10 years from date of delivery to the user b Only during the time the equipment has been maintained on a regular basis as prescribed by Advanced Instruments If the user is in an area which has no local service Advanced Instruments may require a local service person understood to mean the person actually per forming the hands on service of the equipment to attend and pass a reasonable maintenance and repair course c Only during the time when the user has not altered the equipment in any way without written approval from Advanced Instruments 48 Warranty amp Warranty Duties d Only during the time when the user has not loaned leased or
28. he plunger to load a 20 uL sample 3 Visually inspect the sample If there are any large voids or bubbles in the sample expel the sample and load a bubble free sample 4 Blot the sides of the loaded sample cell with a soft no lint non ionic paper tissue to remove any clinging droplets Then blot the end of the cell tip to remove any fluid protruding beyond the tip Be careful not to wick out any of the sample The meniscus remaining may be MENISCUS BEVEL SAMPLE CELL TIP TEFLON PLUNGER A a _W Before Blotting Acceptable Figure 3 Sample Cell Tips and Sample Levels 11 Advanced Osmometer Model 3300 User s Guide 12 slightly concave but the sample must be slightly longer than it is wide See figure 3 5 Remove the chamber cleaner from the sample port and discard 6 Holding the sampler by the barrel and letting the filled sample tip follow the guide groove into the sample port rest the sampler within the operating cradle and beneath the cradle top 7 To start the test push the entire operating cradle down until it reach es a positive stop Your instrument will run the test for approximate ly one minute and display the result in the format Osmolality xxx mOsm 8 Record the results and raise the operating cradle to a positive stop 9 Remove the sampler from the operating cradle 10 Insert a clean dry chamber cleaner into the sample port and rotate it four or five
29. iance with the requirements of UL 3101 1 Ist Edition Electrical Equipment for Laboratory Use Part 1 General Requirements The US in the lower right of the ETL mark demonstrates this listing This product has been listed by ETL testing laborato ries as being in compliance with the requirements of CAN CSA C22 2 No 1010 1 92 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use General Requirements Including Amendment Two The C in the lower left of the ETL mark demonstrates this listing This product meets the intent of Directive 89 336 EEC for Electromagnetic Compatibility Compliance was demonstrated using the following standards as listed in the Official Journal of the European Communities Consult the Declaration of Conformance certificate shipped with the product for the latest update EN 61326 1998 Group 1 Class B Electrical Equipment for Measurement Control and Laboratory Use This product meets the intent of Directive 73 23 EEC the Low Voltage Directive Compliance was demon strated using the following standards as listed in the Official Journal of the European Communities Consult the Declaration of Conformance certificate shipped with the product if required for the latest update EN 61010 1 1993 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use General Requirements Including Amendment Two 1995 Appendix
30. ibration and normal testing may be continued 23 Advanced Osmometer Model 3300 User s Guide Notes 24 EJ Troubleshooting amp Service This chapter contains very basic information to help you solve problems that might arise with your osmometer Please read all instructions very carefully and if a solution cannot be found in this guide contact Advanced Instruments for Hot Line Service Service amp maintenance cautions Do not perform any service or maintenance yourself except as detailed in the User s Guide Unplug the power cord prior to opening or removing cov ers or else you may be exposed to electric shock exces sive temperatures or mechanical hazards Performing service or maintenance not detailed in the User s Guide with or without a Service Manual should only be done by a qualified service technician Never restrict airflow into or out of the product Occasion ally check the air vents for blockage Wipe the exterior of the product with a soft damp cloth as needed Using cleaning products other than those specified may discolor or damage the finish If the product requires service for any of the following rea sons unplug the product from the electrical outlet and refer service to a qualified service technician The power cord extension cord power strip or power input module is damaged 25 Advanced Osmometer Model 3300 User s Guide 26 Liquid has been spilled into
31. in are determined and displayed in place of the sample osmolality Use the following pro cedure to run the test 1 On entry the probe bin test will display Probe Bin Test YES Ready CANCEL 2 Press YES to start the test When YES is pressed the display changes to Insert 50 mOsm Sample CANCEL 3 To continue load a sample from a freshly opened ampule of Advanced 50 mOsm standard and run the test using the sample test procedure detailed in Chapter 2 4 Cooling Sample is displayed as in a normal test then the sample probe resistance in ohms is dynamically displayed as the sample is cooled At the end of each test the final display will be the test result in ohms and the sample probe bin e g 5801 02 Ohms Bin 6 5 Record your results in the Service Log before running another test or canceling to the diagnostic menu 6 Raise the operating cradle to complete the test 7 When the operating cradle is raised YES Ready CANCEL Troubleshooting amp Service provides the opportunity to either run another 50 mOsm probe test or finish cancel the procedure Run two more bin tests and average the results of the three to determine your bin number 8 Press both soft keys and release them simultaneously or press the CANCEL soft key to exit to the menu Barcode Test This test performs a continuous check of the barcode port 1 Press the START key to enter the test 2 A
32. ion and coefficient of variance If any test errors are stored in the range you select they will be filtered out of the calculations To use this option follow this procedure l At Osmometer Ready the two multifunction soft keys are labeled CALIB and DIAG respectively Press the soft key labeled DIAG to enter the diagnostic test menu While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX soft key to cycle through your choices until you see 2 Statistics Press START to enter the statistics mode Your instrument will dis play the number of tests to be considered when computing statistics Press the right INDEX soft key to index to the number of results that you wish to consider NOTE If you request calculations for more samples than are stored in the recall buffer you will receive incorrect results Press the left START soft key to display and print if using exter nal printer or serial port the average of the selected number of last results Instrument Operation Press the left START soft key again to display and print the stan dard deviation of the selected number of last results Press the left START soft key a third time to display and print the coefficient of variance of the selected number of last results To return to the diagnostic menu press the two multifunction soft keys simultaneously To return
33. ium chloride were to completely dissociate into two ionic species Na and Cl in 1 kg of water the freezing point would be depressed by 3 716 C However dissociation is never complete Interference between solute molecules reduces dissociation by a factor called the osmotic coefficient In a simple solution such as glucose or sodium chloride in water the freezing point can be measured and the unit concentration easily deter mined from an equation or a reference table However the equation is unique for each solute In a more complex solution all ionized and non dissociated species contribute to the freezing point depression and the concentration of each solute cannot be easily determined Each of the colligative properties has a similar problem and though each of the colligative properties changes in direct proportion to the solute concentration each requires a different mode amp unit of measure ment Osmolality is a common unit of concentration measurement that can be used to relate all the colligative properties to each other and to other concentration units Because of its universality most osmometry applications regularly use osmolality expressed as mOsm kg H O as the common unit of concentration rather than applying further conver sion factors Instrumentation Advanced Osmometers are devices for the determination of the concen tration of solutions by means of freezing point measurement Advanced Osmometers utilize hig
34. ly designed to support unidirectional com munication with an external device At this time there is no pro tocol for bidirectional communication 19 Advanced Osmometer Model 3300 User s Guide 20 For a sample RS 232 Port Setup please see the Supplemental RS 232 Information Appendix at the end of this user s guide Instrument software update Your instrument software contained in two flash EPROMs on the main processor board may be updated via the RS 232 port Such software updates are made available as new features and other improvements are added during the life cycle of the instrument For information on the availability of software updates contact Advanced Instruments Inc or an authorized distributor See Chapter 4 page 23 For directions on performing the RS 232 serial port software upgrade consult the docu mentation supplied with the upgrade package Ej Calibration This chapter describes the procedure for calibrating your instrument If you have questions or problems regarding the calibration procedure please consult Chapter 4 Troubleshooting amp Service Calibration procedure Calibration of the Model 3300 is a simple procedure that requires no adjustment of the instrument by the user Simply run the menu driven calibration program requiring the testing of standards at each of two calibration points If the repeata bility is acceptable on these calibration points the instrument automatically performs in
35. n languages 5 To lock in the selected language and return to the diagnostic menu press the two soft keys simultaneously 6 Press the two soft keys again simultaneously to return to Osmometer Ready Using a barcode scanner Part 330016 with the Model 3300 The barcode option provides a means of entering an identification code with an internally decoding barcode scanner connected to the barcode port To interface with the Model 3300 the barcode scanner must be programmed as follows referring to the scanner users guide 10 msec Intercharacter Delay Disable Line Feed Suffix No Parity 1200 BAUD Rate 8 Data Bits When the barcode scanner part 330016 is connected to the Model 3300 the instrument operator may scan an identifying barcode before introducing a sample NOTE The barcode configuration may work with many different scanners but not all are recommended and supported by Advanced Instruments For information on which scanners are supported by Advanced Instruments please contact Advanced Instruments customer service department NOTE To avoid possible damage to your instrument or the barcode scanner turn off the instrument power before connecting to l f the barcode port 18 Instrument Operation If you introduce a sample without a prior barcode scan the test results will display without further identification If you scan a barcode the Model 3300 will display Successful Scan Cancel on the firs
36. o not attempt to perform electrical work if you are not fully qualified This manual is not a substitute for electrical training Symbol conventions AN A A Atas F The exclamation point within an equilateral triangle is intended to alert the user to the presence of important oper ating and maintenance servicing instructions in the litera ture accompanying this product The lightning flash with arrowhead symbol within an equi lateral triangle is intended to alert the user to the presence of uninsulated dangerous voltage within the product s enclosure that may be of sufficient magnitude to constitute risk of electric shock to persons The static symbol within an equilateral triangle is intended to alert the user to the presence of internal components that could be damaged by static electricity This static symbol is intended to alert the user to the pres ence of a specific component that could be damaged by static electricity ix Advanced Osmometer Model 3300 User s Guide Nv This symbol indicates the presence of alternating current AC E This symbol indicates the presence of a fuse D This symbol indicates the presence of protective earth ground General cautions This product should be operated only with the type of power source indicated on the product s electrical ratings label Refer to the instal lation instructions included with the product If the power cord provided is replaced for any
37. omer Service Department e 800 225 4034 toll free within the USA and Canada e US 781 320 9000 elsewhere e 781 320 3669 fax Thermal Printer with Interface Cable Operation Manual 330555 Thermal Paper Roll and Printer Power Supply Note Contact customer service to order the appropriate power supply Note 330990 upgrade kit comes with a 3 meter serial cable 330053 vi Notes fi Calibrators amp Standards To order calibrators and standards contact the Advanced Instruments Customer Service Department 800 225 4034 toll free within the contiguous 48 states e US 781 320 9000 elsewhere e 781 320 3669 fax DESCRIPTION PART NO Clinitrol Reference Solution 10 2 mL ampules 3MA029 Protinol 3 Level Protein Control Kit 9 3 mL bottles 3 of 3MA028 each level 5 Value Osmolality Linearity Set 10 2 mL ampules 2 of 3LA028 each value 50 mOsm kg Calibration Standard 10 2 mL ampules 3MA005 850 mOsm kg Calibration Standard 10 2 mL ampules 3MA085 vii Notes viii Mi Safe Use To reduce the risk of bodily injury electric shock fire and damage to your instrument please read and observe the pre cautions in this User s Guide Ifthe product is used in a manner not in accordance with the equipment design operating instructions or manufac turer s recommendations the operation of the product may be impaired to the extent that a safety hazard is created D
38. ontrols contact Advanced Instruments or an authorized representative Clinitrol 290 Reference Solution Part No 3MA029 We recommend that you run samples of Clinitrol 290 Reference Solution daily to check your instrument operation and confirm your cal ibration You should also run the Clinitrol 290 Reference Solution when you receive erratic results Doing so will allow you to verify proper operation or recognize and diagnose problems promptly Protinol Part No 3MA028 Advanced Protinol protein based controls at 240 280 and 320 mOsm 5 mOsm provide control values that closely bracket those of most serum samples These controls are formulated to mimic the freezing characteristics of actual serum samples and they can be used to verify Instrument Operation the precision and reliability of your osmometer results Protinol should be used once every shift Advanced Five Level Linearity Set Part No 3LA028 The Advanced Linearity Set contains five controls 100 500 900 1500 and 2000 mOsm Use this set to verify or establish the reportable range of patient test results Sample test procedure Use the following procedure to run sample tests 1 Snap a sample tip into place on the sampler The sample cell must be straight and firmly seated Be careful not to crack the sample tip 2 Depress the sampler s plunger and insert the sample tip at least 4 inch 6 mm below the surface of the fluid to be tested Gently release t
39. or the Configure menu and click on the OK button Bits per second See user s guide Data bits 8 Parity None Stop bits 1 Flow control None Select the Settings menu Chose TTY for the Emulation option Select ASCII setup Set the Sending option to Echo Typed Characters Locally 5 6 7 8 9 You should at this point see all instrument output in the Hyperterminal window of your computer screen Set the Receiving option to Wrap Lines that Exceed Terminal Width Null Modem Cable Shield connects to metalized connector hous ing at both ends 52 Appendix F Service Log Model Model 3300 Serial Number Software Revision Sample Probe Number Block Probe Number Problem Symptom 53 Advanced Osmometer Model 3300 User s Guide Date Problem Symptom Action 54 E Index B barcode port barcode scanner barcode test beeper test C calibration calibration gauge calibration setscrew calibration standard chamber cleaning Clinitrol 290 Reference Solution concentrative properties controls crystallization temperature D data capture date diagnostic test menu diagnostics display print test E error messages errors event record 9 18 31 31 21 34 34 29 21 33 10 33 10 xvii 17 15 13 28 31 28 37 12 32 F freezing point xiii XV freezing point depression
40. ord the results before operating your instrument Step 2 Unpack your instrument To unpack your osmometer take the following steps a Carefully unpack your osmometer accessories and supplies and inspect them for shipping damage Use the enclosed packing list to verify that all items have been received Advanced Osmometer Model 3300 User s Guide b Save your osmometer s shipping boxes and packaging material in case future transport of the instrument becomes necessary c If any item on the packing list appears to be missing from your ship ment please search carefully through and under all packing materi als If the item is not found notify your receiving department imme diately Advanced Instruments can only be responsible for items reported missing within 10 days of a shipment s arrival d If you receive any damaged items save the cartons and packing material those items came in for inspection by the insurer The carri er dealer and Advanced Instruments must be notified within 24 hours in order for your warranty and insurance to apply Have the transportation company inspect items fill out a Report of Concealed Damage and file your claim Then notify Advanced Instruments immediately for repair or replacement e Fill out the postage paid U S A Only warranty card enclosed Mark the appropriate boxes if you wish to receive additional informa tion Customers outside of the United States may fax the
41. peat the sample in question For repeat runs use additional samples from the same source Keep the cooling chamber clean between tests Never inject any thing into the cooling chamber Recall results The recall results feature of the Model 3300 allows you display or print the results of your last 30 tests If you need to see any or all of these results take the following steps 1 At Osmometer Ready the two multifunction soft keys are labeled CALIB and DIAG respectively Press the soft key labeled DIAG to enter the diagnostic test menu 2 While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX keypad to cycle through your choices until you see 1 Recall Results 13 Advanced Osmometer Model 3300 User s Guide 14 Press START to enter the recall results mode Your instrument will display the data of the most recent test result 4 Press the right soft key to see the result of the next to last test result 5 Continue in this manner until you have recalled the necessary results 6 To return to the diagnostic menu press the two soft keys simultane ously 7 To return to Osmometer Ready again press the two function key pads simultaneously Statistics The statistics option allows you to recall the stored results from a selected last number of tests stored in the recall buffer and calculate their average standard deviat
42. play is in the form off Blk NNNN NN ohm where off indicates that a target temperature has not yet been selected Blk indicates that the block probe channel is being tested NNNN NN is a numeric readout of the probe and ohms indicates the units of the read out These readings are updated continuously Pressing the left function soft key sequentially changes the target cool ing block temperature from off to 1 C to 0 C to 1 C to 2 C to 4 C to 8 C to 12 C to off etc Pressing the right function soft key sequentially changes the channel and readout units from probe resistance in ohms to probe temperature in tenths of a degree Celsius to cooling block duty cycle NN on to probe resistance in ohms etc 29 Advanced Osmometer Model 3300 User s Guide 30 CAUTION The temperature values displayed are based on the block AN probe resistance which the instrument is configured for Thus if the block probe bin has been incorrectly set both the displayed temperatures and resistances will be incorrect as well as the actual temperature of the cooling block Press both soft keys and release them simultaneously to exit to the menu Probe Bin Test This test is used to determine the resistance of the sample probe at a specific temperature Itis essentially the same as any other sample test except that the sample probe resistance and correct b
43. printer with the Model 3300 in this chapter RS 232 port Figure 2 The RS 232 port allows you to output your instrument s data messages to an external device such as a computer and to upgrade your instrument software For more detailed information dealing with the RS 232 port please read the sections titled Using the Model 3300 s RS 232 Port and Instrument Software Update found later in this chapter Instrument Operation Barcode port Figure 2 A D type 15 pin barcode port is provided in the back of the 3300 for connecting and providing power to such a device For proper operation the barcode port requires a 1200 bps RS232 level signal providing asynchronous serial data containing start bit 8 data bits 1 stop bit and no parity Direction to reader from reader common Table 2 Barcode Port Connections A suitable RS232 barcode scanner is available from Advanced Instruments Inc To interface with the 3300 the barcode scanner must be programmed as follows referring to the scanner users guide 10 msec Intercharacter Delay Disable Line Feed Suffix No Parity 1200 BAUD Rate 8 Data Bits Supervisor operator keyswitch Figure 2 The supervisor operator keyswitch allows certain instrument functions to be locked The Supervisor keyswitch position is required for re calibration and for access to the following diagnostic menu items Set Probe Bin s Set Serial Rate Serial Comm Set and Select Lang
44. resold the equipment to any third party 2 Reasonable use is use a According to the instructions sup plied by Advanced Instruments assuming English reading person nel or supervision If neither the supervisor nor the operator reads English the user agrees to obtain accurate translations of the instru ment labels instructions user s guides and or manuals provided Under direct on the job supervi sion of the supervisor or other pro fessional in charge In which there are no known defects or uncorrected repairs Only for the purpose stated in the instructions provided with the instrument In which the equipment has been maintained according to the instructions provided 3 Immediate notification is a Recognition that time is of the essence when any accident mal practice or product liability arises which involves Advanced Instruments equipment Notification to Advanced Instruments immediately the same day if possible in the event of injury to any person in circum stances involving Advanced Instruments equipment in which Advanced Instruments might be named as a defendant in any form of litigation Allowing Advanced Instruments or its representatives immediate full and thorough examination of Advanced Instruments equipment and all records pertaining to such equipment 49 Advanced Osmometer Model 3300 User s Guide Notes 50 Appendix E Supplemental RS 23
45. se the right keypad to toggle between hours and minutes and the left keypad to change the number of hours or minutes dis played c Press both soft key buttons to save the new time setting and return to the diagnostic test menu Set Probe Bin s To reset the block and sample probe bin numbers take the following steps l At Osmometer Ready the two multifunction soft keys are labeled CALIB and DIAG respectively Press the soft key labeled DIAG to enter the diagnostic test menu While you are within the diagnostic test menu the soft key labels will change to START and INDEX Use the INDEX soft key to cycle through your choices until you see 3 Set Probe Bin s Press START to enter the selection menu Press the right INDEX soft key to advance the probe bin or press the left NEXT soft key to advance to the next probe bin Be sure to record the new bin settings in the Service Log at the back of this user s guide Press NEXT soft key to advance to the save options Select YES to save the new setting or NO if no change was made Instrument Operation Data Capture To change the RS 232 output between standard mode and capture mode take the following steps DH vn A W NOTE With Capture selected freezing curve output data will be presented to the serial port in real time This data is updated every 50 msec after crossing 0 C and may be captured with any RS 232
46. stic and parts warranted by their makers Appendix C Regulatory Notices e This product has been designed and manufactured in accordance with U S Canadian and European regulatory requirements as outlined below Modifications made to this product that are not expressly approved in writ ing by the manufacturer will void the user s authority to operate this product previously issued factory approvals and the user s rights under the warranty e The distributor or dealer may have applied additional local national or international approvals to this product Consult the distributor or dealer for more information and documentation e Connections to this product must be made with shielded cables Use of non shielded cables may violate RFI EMI limits Symbol Conventions This symbol indicates conformity to relevant European directives This symbol indicates the product was tested to conform to relevant i Canadian and U S safety standards by Intertek Testing Services NA i eTe Inc The ETL mark is approved in the United States as a Nationally Recognized Testing Lab NRTL by OSHA and in Canada by the Standards Council of Canada 45 Advanced Osmometer Model 3300 User s Guide Regulatory approval type U S Safety Canadian Safety EC Declaration of Conformity EMC EC Declaration of Conformity Low Voltage Description This product has been listed by ETL testing laborato ries as being in compl
47. t YES Ready CANCEL you may press YES once the bar code scanner is connected or press CANCEL to return to the main menu After you press YES you may begin scanning barcodes If your code will not scan check the manufacturers user s guide to verify correct setup of the scanner for your symbologies 3 Press CANCEL or both keys to exit to the test menu Display Print Test This menu item is a simple check of the display serial port and printer if connected On entry a single line of characters will be displayed and sent to the serial and parallel ports The characters displayed have been chosen to exercise every dot in the character matrix This makes it possible to distinguish any dots that no longer work on the printer if connected as well as on the display Press both soft keys and release them simultaneously to exit to the menu Beeper Test This menu item exercises the beeper On entry this test displays ON Beeper OFF Press the ON soft key to cause the beeper to beep repeatedly press the OFF soft key to stop the beeper Press both soft keys and release them simultaneously to exit to the menu 31 Advanced Osmometer Model 3300 User s Guide 32 Solenoid Test This test is used to exercise the freeze pulse solenoid On entry into this test press the IMPACT soft key for a single impact or the BUZZ soft key for a set of repeated impacts If either or both of these do not occur when
48. t is important that you read and adhere to the instructions in this section For information on calibration see Chapter 3 Calibration Hazardous material cautions e WARNING The specimens used in this product may be hazardous to your health and to the health of others Follow national and local recognized safety standards and or protocols for handling and disposing of such speci mens e Ifa hazardous material is spilled on or inside the equip ment the user is responsible for taking the appropriate decontamination steps as outlined by the user s national safety council or equivalent agency for hazardous materi als and those policies and procedures established within the user s place of business e To avoid injury or fire hazard do not operate this product in an explosive atmosphere Function of major components The major components of the Model 3300 Micro Osmometer are an operating cradle a sample port to pre cisely position each sample for the osmolality test a high precision thermistor probe measurement and control cir cuitry and a message display panel A sample handling Advanced Osmometer Model 3300 User s Guide Display Operating Panel oo Cradle Multifunction Soft keys Sampler Chamb er Cooling Cleaners Chamber Sy inside Sample Tips Figure 1 Model 3300 Components and Controls pipette is also used in the testing process The function of many of these parts is described below
49. t line and the decoded barcode on the second line During that display the instrument operator may introduce a sample for test and identification with the decoded barcode or may cancel and either re scan or introduce a sample without barcode identification Using a printer with the Model 3300 The 25 pin output printer port on the back of the Model 3300 allows the use of most standard printers For information on ordering a compatible printer contact the Customer Service Department at Advanced Instruments See page v for ordering information The use of a printer cable and or printer power cable other than supplied by Advanced Instruments may result in excessive electromagnetic noise and unintended interference with other devices Using the Model 3300 s RS 232 port The RS 232 port allows you to output to an external device such as a computer and to update your instrument software Almost every item of information displayed by your instrument is also transmitted over the RS 232 port including test results all error messages and most display data from the diagnostic menu The default serial data rate for communications is 9600 bps bits per second though you may alternatively select 1200 2400 4800 and 19200 bps The DB 9 RS 232 port on your instrument conforms to the DTE EIA 232 standard and can reliably communicate over shielded cable up to 10 meters in length depending on the baud rate you use Note Your instrument is on
50. ternal calibration The calibration procedure is as follows Note that maintaining and loading accurate uncontaminated standard samples is extremely important 1 At Osmometer Ready press the left soft key labeled CALIB to initiate the calibration process The calibration program will prompt you to run a sample of the 50 mOsm calibration standard 2 Run the 50 mOsm calibration standard as you would an actual sample see Chapter 2 Operation When the instrument completes each test and reports the results not necessarily the exact standard value raise the operating cradle remove the sampler and clean the chamber as rec ommended in Chapter 2 21 Advanced Osmometer Model 3300 User s Guide 22 3 The calibration program will prompt you for a second 50 mOsm cal ibration standard Run the second 50 mOsm calibration standard When the test finishes your instrument will display a result and prompt you for an 850 mOsm calibration standard Run your first 850 mOsm calibration standard as described above The program will report a result and request another 850 mOsm cali bration standard Continue in this manner until the instrument dis play reads Calibration complete The program can require any where between three and six 850 standard samples depending on the repeatability of the results Verify the calibration by running a Clinitrol 290 reference solution before running unknown samples If
51. the appropriate soft key has been pressed contact Advanced Instruments for Hot Line Service Press both soft keys and release them simultaneously to exit to the menu Key Test This menu item allows individually testing the switches in the soft keys On entry this test displays PRESS over each soft key When each soft key is pressed the beeper should sound and an asterisk should appear above the pressed soft key to indicate proper operation Event Record On entry this menu item enables downloading the internally stored event record to both the serial and parallel ports When ready press YES to download Press CANCEL or both soft keys and release them simultaneously to exit to the menu Toggle the INDEX soft key to select another menu item or press both soft keys and release them simultaneously to exit from the diagnostic menu As necessary on exit from the diagnostic menu the power up tests will automatically begin and should finish with the display Osmometer Ready Fuse replacement If you determine that your instrument is not functioning because of blown fuses you will need to replace the fuses using the following pro cedure 1 Switch the power switch to the off position and disconnect the power cord Troubleshooting amp Service Use a small flat bladed screwdriver or similar tool to pry out the fuse holder Double check the values marked on the fuses The Model 3300 will automatically adjust
52. the interior of the product A foreign object has fallen into the product The product has been dropped or damaged by a falling object There are noticeable signs of overheating or a burning odor The product does not operate normally when you follow the oper ating procedures The main supply fuse s or any internal fuse s continually fail A discharge of static electricity from contact with the human body or other conductor may damage system boards or static sensitive devices Never perform internal maintenance without following rec ommended static protection procedures The product is equipped with operator accessible fuses Ifa fuse blows it may be due to a power surge or failure of a component Replace the fuse only once If the fuse blows a second time it is probably caused by failure of a component part If this occurs refer service to qualified service personnel Always replace the fuse with one of the same rating voltage and type Never replace the fuse with one of a higher current rating When servicing the product use only factory specified parts WARNING When returning this product for service or shipping this product to a second location remove all hazardous AN specimens and decontaminate the product before pack aging for shipment If the product cannot be deconta minated consult with your shipping agent on appropri ate packaging and marking Troubleshooting amp Service Obtaining service Be
53. tically sent to the instrument s display Display panel Figure 1 The display panel displays e Test status and results e Status messages when the instrument is turned on or when functions are selected e Pertinent error messages when fault conditions occur Messages longer than 24 characters wrap to the second line Multifunction soft keys Figure 1 Other than starting osmolality tests which are initiated by the operating cradle all operator communication to the Model 3300 is accomplished by means of the two multifunction soft keys Advanced Osmometer Model 3300 User s Guide BAR CODE i 5 250V FUSES ig er Ses SUPERVISOR DISCONNECT POWER BEFORE REPLACING FUSES PRINTER POWER PRINTER i g KEYSWITCH DOAbIMvO 15VDC EARTH GND Figure 2 Model 3300 Back Panel Power module Figure 2 The power module on the rear of the instrument contains the following components e Power switch The rocker style power switch controls the power to the instrument e Power cord connector The power cord connector accommodates a power cord suitable for the power available e Fuse holder amp fuses The fuse holder contains the instrument s necessary fuses For instruc tions on replacing fuses see Chapter 4 Printer port Figure 2 The printer port on the rear panel allows you to use a printer in conjunc tion with this instrument For instructions on using a printer with this instrument see Using a
54. to obtain Hot Line Service Fan Driver Failure Key Input Timeout Lift Operating Cradle Try restarting your instrument If this does not solve your problem contact Advanced Instruments for Hot Line Service This message is caused either by a button being pressed continuously or a keypad malfunction Restart your instrument and if the problem persists contact Advanced Instruments for Hot Line Service Raise the operating cradle to a positive stop Restart your instrument if necessary If this does not solve your problem contact Advanced Instruments for Hot Line Service Troubleshooting Table Lift Operating This message along with audible beeps Cradle indicates the test sample was left in the sam ple chamber for an extended period Lift cra dle remove sample and clean chamber The final result will appear only for 10 seconds once you lift the cradle Clean Sample Chamber No Plateau Your instrument was unable to detect a Detected freezing plateau and was therefore unable to give a result Check your technique and try again If the message persists obtain Hot Line Service Parameter RAM This informative message tells you that a Failed or New new software version has been installed Software Version since you last powered the instrument or that the information stored in parameter RAM has been corrupted The instrument will reload factory defaults
55. to the sam ple port rotate it four or five times clockwise and counter clock wise and withdraw 3 Repeat with a dry chamber cleaner Insert and leave a clean dry chamber cleaner in the sample port until the next sample is to be tested Sample plunger replacement and verification To ensure proper instrument operation you should replace the plunger tip of the sampler every 500 tests or every package of sample cells To replace the plunger tip use the following procedure 1 Unscrew the calibration gauge and key 2 Rotate the sampler shaft until the calibration setscrew appears beneath the access hole in the side of the sampler body 3 Place the key end of the calibration gauge in the access hole and turn counter clockwise to loosen the setscrew 4 Carefully remove the old sampler plunger wire 5 Place a sample tip on the sampler to help you place new wire cor rectly 6 Slip the sampler plunger wire into the sample cell so the Teflon plunger tip protrudes about 1 16 or 1 6 mm from the end of the sample cell 7 Using the key end of the calibration gauge push the plunger into the sampler as far as it will go Troubleshooting amp Service 8 Tighten the calibration Calibration setscrew with the calibra Gage amp Key tion gauge 9 Screw the calibration gauge and key back into the top of the sampler Shaft Shaft Bushing Fingergrip Your 20 uL sampler is now calibrated and re
56. uage If a restricted function is attempted while Supervisor Operator keyswitch is in the operator position a Supervisor Key Needed message is dis played and may be cleared by pressing both function buttons simultane ously before selecting any other function To utilize the supervisor operator keyswitch you must use the key sup plied with your instrument Advanced Osmometer Model 3300 User s Guide 10 Sample handling The sampling system kit supplied with your Model 3300 contains a spe cial sampler and a starter supply of disposable sample cells and cham ber cleaners Each kit contains re order information Be sure to replace the sampler plunger with each new test kit 500 sam ples For instructions on this procedure see Sample plunger replace ment and verification in Chapter 4 AN The Model 3300 measures the osmolality of a sample inside the sample cell while on the pipette This feature requires sample cells specially designed to match both the sampler and the Model 3300 Do not use any other sample handling system with your Model 3300 osmometer and do not use the Model 3300 sampling system with any other laboratory procedure Standards amp controls Advanced Instruments offers and recommends the use of specific stan dards and controls with your Model 3300 osmometer Each type may be used to assess a different aspect of your instrument s performance For information on obtaining these standards and c
57. vanced Instruments or Attention is called to the user s responsibility regarding its authorized service representatives Nor does this reasonable use useful life of the instrument and immediate warranty extend to any consequential damages caused by notification of accident outlined in the warranty duties sec defects or improper use or secondary damage resulting tion of the user s guide and or service manual from prior problems No person is authorized to assume for us any other lia The above provisions do not extend the original war bility in connection with the sale or service of this equip ranty period of any instrument or part thereof which has ment been replaced or repaired thereunder Advanced Instruments reserves the right at any time Remedies made under the terms of this warranty in no without notice to make changes in price color material case include any obligation or responsibility for transporta specifications and models without incurring liability to tion charges or arrangements modify products previously produced and to add or discon All results calibrations effectiveness medical diagno tinue models and accessories ses and interpretations are the responsibility of the user This warranty gives you specific legal rights and you THIS WARRANTY IS IN LIEU OF ALL OTHER may also have other rights which vary from state to state By accepting and operating this instru ment the user and Advanced Instrum
58. warranty card to 781 320 8181 Step 3 Obtain additional items Soft no lint non ionic paper tissues are needed for wiping the sample cells prior to testing Please be sure that you have an adequate supply on hand before attempting to run tests on your instrument Step 4 Power up your instrument Turn your instrument on by pushing the rocker style power switch on the instrument s back panel into the on position Each time your instrument is turned on it automatically runs a one minute setup and self diagnostic program During these diagnostics your display and printer if in use display critical instrument information such as software version and block and sample probe bin numbers The first time you power up your osmometer record the software revision and Installation amp Setup block and sample probe bin numbers in the Service Log at the end of this user s guide Maintaining a record of this information will facilitate any necessary service When the self diagnostics have been completed and the instrument is ready for testing the display reads Osmometer Ready NOTE To avoid any misunderstanding that might arise due to an unfamiliar display language all displayed messages may be changed from English to several other languages For lan guage selection instructions see Chapter 2 CAUTION Ifa power interruption occurs turn the instrument off at once Leave it turned off for at least 5 seconds after
59. x B Product Specifications Electrical Voltage Frequency Fuses 2 Power Memory backup Sample Volume Sample Capacity Readout Units Range Linearity Resolution Repeatability Communications Storage Temperature Operating Temp ambient Drift 100 to 130 VAC or 200 to 250 VAC 50 or 60 Hz 250V time delay Type T 1 25 Amp 100 130 VAC 1 Amp 200 250 VAC 100 Watts Integral lithium cell two years minimum 20 pL Single sample 2 line digital display mOsm kg H O 0 to 2000 mOsm kg H O Within 1 of straight line between calibration points of 50 and 850 mOsm Within 1 5 above 850 mOsm 1 mOsm kg H O 2 mOsm kg H O 1 S D between 0 and 400 mOsm kg H20 0 5 1 S D between 400 and 2000 mOsm kg H O DTE EIA 232 V 24 RS 232 parallel printer and optional barcode scanner 40 to 160 F or 40 to 70 C 64 to 95 F or 18 to 35 C Less than 1 digit per month plus 1 digit for every 9 F or 5 C ambient temperature change Advanced Osmometer Model 3300 User s Guide 44 Operating Humidity Startup Time Test Time Dimensions Width Depth Height Weight Net Shipping Warranty Certification 5 to 80 E H non condensing 30 seconds from power on 60 seconds per sample inches centimeters 10 5 27 13 33 15 5 39 pounds kilograms 16 7 3 25 11 4 One year limited warranty on workmanship and all parts except glass pla
60. xiii freezing point plateau fuse replacement fuses H heat of fusion Hyperterminal installation K key test L language linearity set M melting point molality molarity multifunction soft keys xvii 32 8 28 XV XVii 51 32 17 11 xvii xvi xvi 55 Advanced Osmometer Model 3300 User s Guide 56 O operating cradle 7 operational requirements 28 osmolality osmolarity osmometry P pipette See sampler plunger tip power switch printer printer port probe bin test Protinol R recall results repeatability RS 232 port S sample freeze error sample plunger sample pre freeze sample preparation sample probe sample test procedure sampler select language service service log set date time XIV XVi xvi xiii Xiv 35 v 19 30 10 13 13 8 19 51 40 34 40 10 11 17 25 53 15 set probe bin s settings solenoid test specifications standards statistics supercooling supervisor operator keyswitch T test time out error time setting troubleshooting U unpacking W warranty 16 15 32 37 10 14 XIV xvii 9 42 15 25 37 47 Chapter Name HH Advanced Osmometer Model 3300 User s Guide Chapter Name HH

The Advanced Micro-Osmometer Model 3300

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